CN104793003A - Blood group serology detection method and automatic detection system for realizing method - Google Patents
Blood group serology detection method and automatic detection system for realizing method Download PDFInfo
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- CN104793003A CN104793003A CN201510151965.5A CN201510151965A CN104793003A CN 104793003 A CN104793003 A CN 104793003A CN 201510151965 A CN201510151965 A CN 201510151965A CN 104793003 A CN104793003 A CN 104793003A
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Abstract
The invention provides an automatic blood group serology detection method and an automatic detection system for realizing the method. The method provided by the invention is convenient and effective, controllably improves the suspension effect of micro liquid and satisfies the operation requirements of blood immune reaction, so that results are more reliable and accurate; an adopted test tube luminous flux detection method is the obtained most widely-used method, can quickly judge experimental results and is high in accuracy rate, high in detection rate of weak agglutination and better in sensitivity. The detection system provided by the invention can quickly judge the experimental results through close cooperation of all parts and is high in accuracy rate, high in detection rate of weak agglutination and better in sensitivity; particularly, a bead adding device obtains a good effect and is high in degree of automation, accurate and quick in bead adding action, so that the vibration intensity of an existing oscillating mechanism is greatly reduced, the suspension effect of the micro liquid is improved, and the operation requirements of the blood immune reaction is satisfied.
Description
Technical field
The invention belongs to and relate to a kind of blood group serology detection method and detection system thereof.
Background technology
The detection method of blood group serology is roughly divided into two classes, and a class is manual method, and a class is self-reacting device method.The principle majority detected is all according to antigen-antibody generation agglutinating reaction, and manual observation or instrument detect state of aggregation thus judgment experiment result.The method of artificial employing comprises test tube method and slide method, and the accuracy of observation relies on the order of accuarcy of experience accumulation and processing procedure, for weak agglutinating reaction, also needs to use other instrument (as microscope) to judge.The detection method of instrument roughly has two kinds, one uses micro-column gel card as carrier, in gel microtrabeculae medium, red cell antigens is combined with corresponding antibodies, through the red blood cell suspension of low-speed centrifugal aggegation in gel top, the red blood cell do not combined with antibody is then sunken to bottom gel, then provides reaction result by image recognition.Another is dull and stereotyped pattern-recognition method, and flat type also has U-shaped or V-arrangement, inagglutinable cell can tumble in be formed centrally cell button, the cell of aggegation then can be paved with the end step of circular hole, then by identifying the image on flat board, thus judges state of aggregation.
The complex operation when shortcoming of manual method is to test in enormous quantities, efficiency is lower, and experimental result needs artificial registration and can not realize robotization, and can not ensure not exist owing to neglecting the human error caused; When manual outstanding cell again, if dynamics is grasped bad, easily destroy weak state of aggregation, thus can not correct result be drawn.The advantage of gel cards is highly sensitive, but price is higher, if bacteriological infection appears in sample, easily occurs false positive results.The speed of dull and stereotyped image method process is very fast, but is not suitable for processing the experiment needing to carry out cell washing, often needs special cell washer device to coordinate.
And, when carrying out blood immunology test by hand, need glass test tube sample and reagent being added standard series to hang vibration again, then observation experiment result.When using self-reacting device to carry out detecting, because reaction tube cross-sectional area is less, liquid fluidity is poor.In order to be mixed by reactant and hang again, need the dynamics of larger vibration.
Simple dependence vibration dynamics solves the method that suspending liquid hangs again, there is following shortcoming:
If 1 vibration dynamics is less than normal, then, under antigen-antibody negative reaction state, the material be attached to bottom centrifuge tube not easily suspends;
If 2 vibration dynamics are bigger than normal, then the positive reaction state of antigen-antibody is easily destroyed, especially weak agglutinating reaction;
3, vibrations are comparatively large, to the assembly reliability of parts and coherence request high.
Therefore, people need to find one more suitably method solve these problems.
Summary of the invention
Technical matters to be solved by this invention is to provide one can judgment experiment result fast, and accuracy rate is high, high to the recall rate of weak aggegation, has blood group serology detection method and the detection system thereof of good sensitivity.
In order to improve the vibration of liquid in microcentrifugal tube outstanding effect again, the present inventor expects the method adding steel ball little bottom the pipe compared with centrifuge tube or other shapes of materials object in centrifuge tube, be specially because centrifuge tube is in oscillatory process, the oscillating body that steel ball or other shapes of materials object etc. help micro liquid to suspend moves bottom centrifuge tube, the surface tension of liquid can be destroyed, drive liquid flow, thus the cell that agglutinating reaction does not occur of centrifuge tube inside is suspended, do not destroy the cell state that agglutinating reaction occurs simultaneously, make result more reliable, accurately, thus, itself and other technologies scheme of the present invention are merged, form technical scheme of the present invention.
The technical solution used in the present invention is:
A kind of blood group serology automated detection method, is characterized in that, comprise following steps:
Step 1), in centrifuge tube, add sample needed for experiment and reagent, put into hydro-extractor after mixing and carry out centrifugal;
Step 2), centrifugal after centrifuge tube is adopted device for detecting luminous flux, carry out first time luminous flux detection, detection whether have haemolysis and write down numerical value;
Step 3), in centrifuge tube, add compared with centrifuge tube pipe bottom the little oscillating body helping micro liquid to suspend, select suitable oscillation frequency, amplitude to carry out vibration to centrifuge tube according to corresponding test type multiple outstanding, carry out luminous flux second time after hanging again and detect and write down numerical value; Because centrifuge tube is in oscillatory process, the oscillating body that steel ball or other shapes of materials object etc. help micro liquid to suspend moves bottom centrifuge tube, the surface tension of liquid can be destroyed, drive liquid flow, thus the cell that agglutinating reaction does not occur of centrifuge tube inside is suspended, do not destroy the cell state that agglutinating reaction occurs simultaneously, significantly reduce the vibrations dynamics of original oscillating mechanism, easily and effectively, the suspension effect of micro liquid is controllably improved, meet the operation requirements of Hemaimmune reaction, make result more reliable, accurate;
The difference of step 4), per sample kind, selected after the corresponding time, then carried out third time and detect and write down numerical value;
Step 5), to suspend in the solution based on inagglutinable cell, stronger absorption is had to the light (as blue light and green glow) through specific wavelength wherein, namely luminous flux is less, and photelectric receiver (photodiode, photoelectric cell etc.) the consequent photocurrent being positioned at solution opposite side can be more weak; If there occurs agglutinating reaction, so most cells drops on bottom centrifuge tube, solution presents transparence, luminous flux is larger, the photocurrent that correspondence detects is larger, for more weak agglutinating reaction (as 1+, 2+), due to several cell aggregations together, can than cell settlement fast of not aggegation (negative reaction), the light flux variations amount that it is detected within a certain period of time and the visibly different principle of variable quantity of negative reaction, set up the luminous flux mathematical model of fractional order reaction (negative, 1+, 2+, 3+, 4+); More subfractionation, also can be divided into the luminous flux mathematical model of (negative, suspicious, 1+, 2+, 3+, 4+).
Step 6), according to above-mentioned steps 2) three numerical value i.e. three photocurrent numerical value of ~ step 4) record and the situation of change of three times, the luminous flux mathematical model based on step 5) determines experiment classification results.
As preferably, centrifuge tube used in described method comprises centrifuge tube body, one end of described centrifuge tube body is longer than the other end, described centrifuge tube body comprises the hole of the inverted rectangular pyramid of six platoons, it is a ball curved surface at the bottom of the hole in described hole, between at the bottom of adjacent two holes, tube wall is inwardly provided with groove, and described groove has five.This centrifuge tube, it comprises the hole of the inverted rectangular pyramid of platoon, therefore when doing centrifugal motion, can not rotate, avoid the generation of accident, because one end of centrifuge tube body is longer than the other end, left-right asymmetry, therefore the position of centrifuge tube can not misplace by testing crew.
As preferably, described step 2) in add the oscillating body helping micro liquid to suspend be steel ball or other shapes of materials object, the diameter of steel ball or other shapes of materials object is at 1.5 ~ 2.5mm, also can select according to centrifuge tube inner chamber area, more optimizedly, select the oscillating body helping micro liquid to suspend to be steel ball, diameter of the steel ball is 2mm.
As preferably, described step 2) device for detecting luminous flux used is included in centrifuge tube in ~ step 4) both sides arrange luminotron and photelectric receiver respectively.
As preferably, the time in described step 4) is set as 1.5min ~ 2.5min, more preferably, is set as 2min.
Another object of the present invention is to provide the automated detection system realizing the method.The technical scheme taked is:
A kind of blood group serology automated detection system, it is characterized in that, described system comprises base, described base is provided with cross bearer, sample rotating disk, reagent rotating disk, peristaltic pump, centrifuge tube shelf, hatch frame, hydro-extractor, camera, vibration evenly mixing device and add pearl device, described cross bearer is provided with bearing and mechanical arm, and bearing and mechanical arm can do left and right directions rectilinear motion on crossbeam, described bearing is provided with suction needle, suction needle can do above-below direction rectilinear motion along bearing, described sample rotating disk and reagent rotating disk are positioned at the below of suction needle, reagent rotating disk and sample rotating disk can individually rotate, service sink in the middle of rotating disk, described centrifuge tube shelf and hatch frame and can do fore-and-aft direction rectilinear motion, described centrifuge tube shelf and hatch frame and be provided with slotted eye for placing centrifuge tube, described vibration evenly mixing device is positioned under cross bearer, add the front of pearl device, described vibration evenly mixing device is provided with oscillator tank, for placing centrifuge tube in described oscillator tank, described oscillator tank is also provided with device for detecting luminous flux, described device for detecting luminous flux comprises luminotron and the photelectric receiver of the both sides being arranged on centrifuge tube, camera is positioned at the rear of vibration evenly mixing device, stays shelves to check for taking response diagram picture.
As preferably, the described pearl device that adds comprises feeding chamber, the in-built oscillating body helping micro liquid and suspend of described feeding chamber, there are several through holes that can pass for oscillating body feeding chamber front portion, one piece of movable push is provided with below feeding chamber, described movable push engages with the motor gear on motor, can seesaw, described motor is arranged on below movable push, in movable push, also correspondence is provided with several through holes that can hold oscillating body, one piece of fixed head is also provided with under movable push, described movable push, feeding chamber and motor are all arranged on fixed head, the front portion also corresponding through hole being provided with several and can passing for oscillating body of described fixed head, described fixed head is arranged on guide rail, can be seesawed by the belt drive be arranged on guide rail, also Optocoupler detection device is housed in the front portion of described fixed head.
As preferably, the described oscillating body helping micro liquid to suspend is steel ball or other shapes of materials object, the diameter of steel ball or other shapes of materials object is at 1.5 ~ 2.5mm, also can select according to centrifuge tube inner chamber area, more optimizedly, select the oscillating body helping micro liquid to suspend to be steel ball, diameter of the steel ball is 2mm.
As preferably, the hole size on described feeding chamber, movable push and fixed head all matches with oscillating body diameter.
As preferably, described Optocoupler detection device is an optocoupler testing circuit.
Detection method of the present invention is according to classical test tube ratio juris, the sample needed for experiment and reagent is added in centrifuge tube (double do reaction cup), hydro-extractor is put into after mixing, by mechanical arm, centrifuge tube is put into special oscillator tank after centrifugation, carry out vibration multiple outstanding, hunting speed is by Electric Machine Control.For improving again outstanding effect, provide a kind of method adding the oscillating body that steel ball (or other shapes of materials object) etc. can help micro liquid to suspend in centrifuge tube, the diameter of steel ball (or other shapes of materials object) is at 2mm, also can select according to centrifuge tube inner chamber area, because centrifuge tube is in oscillatory process, steel ball (or other shapes of materials object) moves bottom centrifuge tube, the surface tension of liquid can be destroyed, drive liquid flow, thus the cell that agglutinating reaction does not occur of centrifuge tube inside is suspended, do not destroy the cell state that agglutinating reaction occurs simultaneously.
The method detecting luminous flux is adopted to judge the state of aggregation reacted, luminotron and photelectric receiver is placed respectively in the both sides of centrifuge tube, because inagglutinable cell suspends in the solution, have stronger absorption to the light (as blue light and green glow) of the specific wavelength sent, the photocurrent that photelectric receiver (photodiode, photoelectric cell etc.) produces is more weak; If there occurs agglutinating reaction, so most cells drops on bottom centrifuge tube, solution presents transparence, luminous flux is larger, the photocurrent that correspondence detects is comparatively large, for more weak agglutinating reaction (as 1+, 2+), due to several cell aggregations together, can than cell settlement fast of not aggegation (negative reaction), the light flux variations amount detected within a certain period of time is obviously different from the variable quantity of negative reaction.Utilize this characteristic, set up the luminous flux mathematical model of fractional order reaction (negative, suspicious 1+, 2+, 3+, 4+).
Owing to have employed technique scheme, the present invention has following technique effect:
By practical test, method of the present invention easily and effectively, controllably improves the suspension effect of micro liquid, meets the operation requirements of Hemaimmune reaction, makes result more reliable, accurate; Adopting test tube luminous flux detection method, is obtain the most widely used method, can judgment experiment result fast, and accuracy rate is high, high to the recall rate of weak aggegation, has good sensitivity.Detection system of the present invention is by the close fit of each several part, can judgment experiment result fast, accuracy rate is high, high to the recall rate of weak aggegation, has good sensitivity, especially add pearl device and achieve good result, automaticity is high, adds pearl action accurately rapid, significantly reduces the vibrations dynamics of original oscillating mechanism, improve the suspension effect of micro liquid, meet the operation requirements of Hemaimmune reaction.
Accompanying drawing explanation
Examples of the present invention will be described by way of reference to the accompanying drawings, wherein:
Fig. 1 is the structural representation of detection system of the present invention;
Fig. 2 is the structural representation adding pearl device in embodiment;
Fig. 3 is steel ball shifting principle schematic diagram in embodiment;
Fig. 4 is the structural representation after adding steel ball in embodiment in centrifuge tube;
Fig. 5 is the vertical view after adding steel ball in embodiment in centrifuge tube;
Fig. 6 is the rule schema that in embodiment, luminous flux mathematical model judges classification results;
In figure: base 1, cross bearer 2, sample rotating disk 3, reagent rotating disk 4, peristaltic pump 5, centrifuge tube shelf 6, hatch frame 7, hydro-extractor 8, camera 9, vibration evenly mixing device 10, add pearl device 11, bearing 12, mechanical arm 13, suction needle 14, service sink 15, centrifuge tube 16, oscillator tank 17, feeding chamber 11.1, movable push 11.2, motor 11.3, fixed head 11.4, motor gear 11.5, guide rail 11.6, belt 11.7, Optocoupler detection device 11.8, steel ball 11.9, centrifuge tube body 16.1, hole 16.2,16.2.1, groove 16.3 at the bottom of hole.
Embodiment
Arbitrary feature disclosed in this instructions, unless specifically stated otherwise, all can be replaced by other equivalences or the alternative features with similar object.That is, unless specifically stated otherwise, each feature is an example in a series of equivalence or similar characteristics.
As shown in Figure 1, a kind of blood group serology automated detection system, it is characterized in that, described system comprises base 1, described base is provided with cross bearer 2, sample rotating disk 3, reagent rotating disk 4, peristaltic pump 5, centrifuge tube shelf 6, hatch frame 7, hydro-extractor 8, camera 9, vibration evenly mixing device 10 and add pearl device 11, described cross bearer 2 is provided with bearing 12 and mechanical arm 13, and bearing 12 and mechanical arm 13 can do left and right directions rectilinear motion on cross bearer 2, described bearing 12 is provided with suction needle 14, suction needle 14 can do above-below direction rectilinear motion along bearing 12, described sample rotating disk 3 and reagent rotating disk 4 are all positioned at the below of suction needle 14, and reagent rotating disk 4 and sample rotating disk 3 can individually rotate, service sink 15 in the middle of two rotating disks, described centrifuge tube shelf 6 and hatch frame 7 and can do fore-and-aft direction rectilinear motion, described centrifuge tube shelf 6 and hatch frame 7 and be provided with slotted eye for placing centrifuge tube 16, described vibration evenly mixing device 10 is positioned under cross bearer 2, add the front of pearl device 11, described vibration evenly mixing device 10 is provided with oscillator tank 17, for placing centrifuge tube in described oscillator tank 10, described oscillator tank 10 is also provided with device for detecting luminous flux, described device for detecting luminous flux comprises luminotron and the photelectric receiver of the both sides being arranged on centrifuge tube, camera 9 is positioned at the rear of vibration evenly mixing device 10, stays shelves to check for taking response diagram picture.
As shown in Figure 2, the described pearl device 11 that adds comprises feeding chamber 11.1, described feeding chamber 11.1 is built with the oscillating body 11.9 helping micro liquid to suspend being specially steel ball, there are several through holes that can pass for steel ball feeding chamber 11.1 front portion, feeding chamber 11.1 is provided with one piece of movable push 11.2 below, described movable push 11.2 engages with the motor gear 11.5 on motor 11.3, can seesaw, described motor 11.3 is arranged on below movable push 11.2, in movable push 11.2, also correspondence is provided with several through holes that can hold steel ball, one piece of fixed head 11.4 is also provided with under movable push 11.2, described movable push 11.2, feeding chamber 11.1 and motor 11.3 are all arranged on fixed head 11.4, the front portion also corresponding through hole being provided with several and can passing for steel ball of described fixed head 11.4, described fixed head 11.4 is arranged on guide rail 11.6, driven can be seesawed by the belt 11.7 be arranged on guide rail 11.6, in the front portion of described fixed head 11.4, Optocoupler detection device 11.8 is also housed.Described pearl is steel ball.The diameter of described steel ball is 1.5 ~ 2.5mm.Hole size on described feeding chamber 11.1, movable push 11.2 and fixed head 11.4 all matches with diameter of the steel ball.Described Optocoupler detection device 11.8 is an optocoupler testing circuit.
As shown in Figure 3, pearl schematic diagram is added for what add pearl device 11, the steel ball 11.9 of the oscillating body suspended as helping micro liquid is first in feeding chamber 11.1, along with movable push 11.2 is goed ahead under the promotion of motor gear 11.5, the through hole of movable push 11.2 and the through hole of feeding chamber 11.1 front end fall into timing steel ball 11.9 in the hole of movable push 11.2, movable push 11.2 moves on, with the through hole on fixed head 11.4 to timing, steel ball 11.9 falls into the through hole of fixed head 11.4, and directly falls in the centrifuge tube 16 that is placed on below.
As shown in Figure 4 and Figure 5, for adding the structural representation after as the steel ball 11.9 of the oscillating body helping micro liquid to suspend in centrifuge tube 16 used in described system and method, described centrifuge tube 16 comprises centrifuge tube body 16.1, one end of described centrifuge tube body 16.1 is longer than the other end, described centrifuge tube body 16.1 comprises the hole 16.2 of the inverted rectangular pyramid of six platoons, 16.2.1 at the bottom of the hole in described hole 16.2 is a ball curved surface, at the bottom of adjacent two holes, between 16.2.1, tube wall is inwardly provided with groove 16.3, and described groove 16.3 has five.This centrifuge tube, it comprises the hole of the inverted rectangular pyramid of platoon, therefore when doing centrifugal motion, can not rotate, avoid the generation of accident, because one end of centrifuge tube body is longer than the other end, left-right asymmetry, therefore the position of centrifuge tube can not misplace by testing crew.After adding steel ball wherein, easily and effectively, controllably improve the suspension effect of micro liquid, significantly reduce the vibrations dynamics of original oscillating mechanism, improve the suspension effect of micro liquid, meet the operation requirements of Hemaimmune reaction.
Principle of work: this detection system, suction needle 14 makes upper and lower rectilinear motion to complete suction sample, application of sample, proportioning, the work such as dilution, centrifuge tube 16 can pick up and mention by mechanical arm 13, to be movedly again centrifuge tube 16 put down to desired location and unclamp, reagent rotating disk 4 is for putting various reagent, for putting various sample on sample rotating disk 3, service sink 15 in the middle of rotating disk can clean suction needle 14, peristaltic pump 5 is in order to complete the cleaning of suction needle 14, the work such as the washing of cell and the discharge of waste liquid, centrifuge tube shelf 6 is booked special centrifuge tube 16 with hatching on frame 7, mixing of sample and reagent can be completed at centrifuge tube shelf 6 place, the work such as the proportioning of solution and mixing, hatch frame 7 place can carry out 37 DEG C hatch experiment, hydro-extractor 8 is undertaken centrifugal by the sample solution being mixed with reagent in centrifuge tube 16, then by mechanical arm 13, centrifuge tube 16 is grabbed vibration evenly mixing device 10 place to shake, hang and complete detection again, adding pearl device 11 is for concussion, steel ball 11.9 is added in the centrifuge tube 16 that sample solution is housed before hanging again.
During concrete enforcement, adopt blood group Virus monitory system as above, for positive definite form, get 5 kinds of samples,
Step 1), in centrifuge tube 16, add sample needed for experiment and reagent, put into hydro-extractor 8 after mixing and carry out centrifugal;
Step 2), centrifugal after centrifuge tube 16 is adopted device for detecting luminous flux, carry out first time luminous flux detection, detection whether have haemolysis and write down numerical value;
Step 3), in centrifuge tube 16, add the steel ball 11.9 that diameter is 2mm, select suitable oscillation frequency, amplitude to carry out vibration to centrifuge tube 16 according to corresponding test type multiple outstanding, multiple outstanding after carry out luminous flux second time and detect and write down numerical value;
The difference of step 4), per sample kind, selects after 2min, then carries out third time and detect and write down numerical value;
Step 5), to suspend in the solution based on inagglutinable cell, stronger absorption is had to the light (as blue light and green glow) through specific wavelength wherein, namely luminous flux is less, and photelectric receiver (photodiode, photoelectric cell etc.) the consequent photocurrent being positioned at solution opposite side can be more weak, if there occurs agglutinating reaction, so most cells drops on bottom centrifuge tube, solution presents transparence, luminous flux is larger, the photocurrent that correspondence detects is larger, for more weak agglutinating reaction (as 1+, 2+), due to several cell aggregations together, can than cell settlement fast of not aggegation (negative reaction), (i.e. the cell of weak aggegation goes down along with time lengthening understands sedimentation the visibly different principle of variable quantity of the light flux variations amount detect within a certain period of time it and negative reaction, solution can become relative transparent a bit, then luminous flux can become large a little), set up fractional order reaction (negative, suspicious, 1+, 2+, 3+, luminous flux mathematical model 4+),
Step 6), according to above-mentioned steps 2) three numerical value i.e. three photocurrent numerical value of ~ step 4) record and the situation of change of three times, namely the decision rule of contradistinction system setting determines experiment classification results based on the luminous flux mathematical model of step 5).The rule citing judged is as Fig. 6.
As can be seen from Figure 6, for the sample solution that luminous flux in 3 luminous flux detection is always very large, be decided to be (4+) sample solution that agglutinating reaction occurs, if reason is that strong agglutinating reaction occurs, the cell meeting sedimentation of aggegation is rapid, and solution is transparent rapidly;
For the 1st time time, luminous flux is very large, in the 2nd time and the 3rd time, luminous flux is also larger, and the solution that the luminous flux result of the 3rd time is larger than the 2nd time, for this solution, be decided to be the sample solution of (3+) of the stronger agglutinating reaction of generation, if reason is that stronger agglutinating reaction occurs, cell major part that stronger aggegation occurs when detecting for the 2nd time is by relatively rapidly sedimentation, and do not have the cell of aggegation floating in the solution, solution has relatively large transparency, along with the prolongation of time, when detecting for the 3rd time, the cell of the stronger aggegation of remaining generation is by further sedimentation, make solution transparent further, luminous flux becomes large,
For the 1st time time, luminous flux is very large, in the 2nd time and the 3rd time, luminous flux the 2nd with the change procedure of result to measure for the 3rd time and sample solution similar of the stronger agglutinating reaction of generation (3+), but overall luminous flux is than smaller solution, be decided to be the sample solution of the more weak agglutinating reaction of generation (1+, 2+), reason is with (3+) sample solution;
For the 1st time time, luminous flux is very large, the sample solution that when measuring in the 2nd time and the 3rd time, luminous flux result is all very little and comparatively stable, be decided to be the sample solution that negative (-) namely agglutinating reaction does not occur, reason is exactly that the solution that causes suspending always in the solution of inagglutinable cell is opaque.
By will the rule of the above-mentioned judgement of Comparative result recorded be tested, can judgment experiment result fast, accuracy rate is high, high to the recall rate of weak aggegation, has good sensitivity, proves this blood group serology automated detection method scientific and effective thus.
By practical test, method of the present invention easily and effectively, controllably improves the suspension effect of micro liquid, meets the operation requirements of Hemaimmune reaction; Adopting test tube luminous flux detection method, is obtain the most widely used method, can judgment experiment result fast, and accuracy rate is high, high to the recall rate of weak aggegation, has good sensitivity.Detection system of the present invention is by the close fit of each several part, can judgment experiment result fast, accuracy rate is high, high to the recall rate of weak aggegation, has good sensitivity, especially add pearl device and achieve good result, automaticity is high, adds pearl action accurately rapid, significantly reduces the vibrations dynamics of original oscillating mechanism, improve the suspension effect of micro liquid, meet the operation requirements of Hemaimmune reaction.
The present invention is not limited to aforesaid embodiment.The present invention expands to any new feature of disclosing in this manual or any combination newly, and the step of the arbitrary new method disclosed or process or any combination newly.
Claims (10)
1. a blood group serology automated detection method, is characterized in that, comprises following steps:
Step 1), in centrifuge tube, add sample needed for experiment and reagent, put into hydro-extractor after mixing and carry out centrifugal;
Step 2), centrifugal after centrifuge tube is adopted device for detecting luminous flux, carry out first time luminous flux detection, detection whether have haemolysis and write down numerical value;
Step 3), in centrifuge tube, add compared with centrifuge tube pipe bottom the little oscillating body helping micro liquid to suspend, select suitable oscillation frequency, amplitude to carry out vibration to centrifuge tube according to corresponding test type multiple outstanding, carry out luminous flux second time after hanging again and detect and write down numerical value;
The difference of step 4), per sample kind, selected after the corresponding time, then carried out third time and detect and write down numerical value;
Step 5), suspend in the solution based on inagglutinable cell, have stronger absorption to the light through specific wavelength wherein, namely luminous flux is less, and the consequent photocurrent of photelectric receiver being positioned at solution opposite side is more weak; If there occurs agglutinating reaction, so most cells drops on bottom centrifuge tube, solution presents transparence, luminous flux is comparatively large, and the photocurrent that correspondence detects is comparatively large, for more weak agglutinating reaction, due to several cell aggregations together, can fast than the cell settlement under not agglutinating reaction i.e. negative reaction state, the light flux variations amount detect within a certain period of time it and the visibly different principle of variable quantity of negative reaction, set up the luminous flux mathematical model of fractional order reaction;
Step 6), according to above-mentioned steps 2) three numerical value i.e. three photocurrent numerical value of ~ step 4) record and the situation of change of three times, the luminous flux mathematical model based on step 5) determines experiment classification results.
2. blood group serology automated detection method according to claim 1, it is characterized in that, centrifuge tube used in described method comprises centrifuge tube body (16.1), one end of described centrifuge tube body (16.1) is longer than the other end, described centrifuge tube body (16.1) comprises the hole (16.2) of the inverted rectangular pyramid of six platoons, (16.2.2) at the bottom of the hole in described hole (16.2) is a ball curved surface, at the bottom of adjacent two holes, between (16.2.1), tube wall is inwardly provided with groove (16.3), and described groove (16.3) has five.
3. blood group serology automated detection method according to claim 1, is characterized in that, described in help micro liquid to suspend oscillating body be steel ball, the diameter of described steel ball is 1.5 ~ 2.5mm.
4. blood group serology automated detection method according to claim 1, is characterized in that, described step 2) device for detecting luminous flux that uses in ~ step 4) comprises the both sides luminotron and photelectric receiver that are arranged on centrifuge tube.
5. blood group serology automated detection method according to claim 1, is characterized in that, the time in described step 4) is set as 1.5-2.5min.
6. blood group serology automated detection method according to claim 1, is characterized in that, in described step 5), never aggegation is divided into feminine gender, these 5 grades of 1+, 2+, 3+, 4+ to weak aggegation to the level of reaction of aggegation.
7. one kind for realizing the blood group serology automated detection system of method described in claim 1 ~ 6 any one, it is characterized in that, described system comprises base (1), described base is provided with cross bearer (2), sample rotating disk (3), reagent rotating disk (4), peristaltic pump (5), centrifuge tube shelf (6), hatch frame (7), hydro-extractor (8), camera (9), vibration evenly mixing device (10) and add pearl device (11), described cross bearer (2) is provided with bearing (12) and mechanical arm (13), and bearing (12) and mechanical arm (13) can do left and right directions rectilinear motion on cross bearer (2), described bearing (12) is provided with suction needle (14), suction needle (14) can do above-below direction rectilinear motion along bearing (12), described sample rotating disk (3) and reagent rotating disk (4) are all positioned at the below of suction needle (14), and reagent rotating disk (4) and sample rotating disk (3) can individually rotate, service sink (15) in the middle of two rotating disks, described centrifuge tube shelf (6) and hatch frame (7) fore-and-aft direction rectilinear motion can be done, described centrifuge tube shelf (6) and hatch frame (7) and be provided with slotted eye for placing centrifuge tube (16), described vibration evenly mixing device (10) is positioned under cross bearer (2), add the front of pearl device (11), described vibration evenly mixing device (10) is provided with oscillator tank (17), for placing centrifuge tube (16) in described oscillator tank (17), (17) are also provided with device for detecting luminous flux to described oscillator tank, described device for detecting luminous flux comprises the luminotron and photelectric receiver that are arranged on centrifuge tube (16) both sides, camera (9) is positioned at the rear of vibration evenly mixing device (10).
8. blood group serology automated detection system according to claim 7, it is characterized in that, the described pearl device (11) that adds comprises feeding chamber (11.1), described feeding chamber (11.1) is built with the oscillating body (11.9) helping micro liquid to suspend little bottom the pipe of comparatively centrifuge tube (16), the through hole that feeding chamber (11.1) front portion has several can supply oscillating body (11.9) to pass, feeding chamber (11.1) is provided with one piece of movable push (11.2) below, described movable push (11.2) engages with the motor gear (11.5) on motor (11.3), can seesaw, described motor (11.3) is arranged on below movable push (11.2), in movable push (11.2), also correspondence is provided with several through holes that can hold oscillating body (11.9), one piece of fixed head (11.4) is also provided with under movable push (11.2), described movable push (11.2), feeding chamber (11.1) and motor (11.3) are all arranged on fixed head (11.4), the front portion of described fixed head (11.4) also correspondence is provided with several through holes that oscillating body (11.9) can be supplied to pass, described fixed head (11.4) is arranged on guide rail (11.6), driven can be seesawed by the belt (11.7) be arranged on guide rail (11.6), in the front portion of described fixed head (11.4), Optocoupler detection device (11.8) is also housed.
9. blood group serology automated detection system according to claim 8, is characterized in that, described in help micro liquid to suspend oscillating body (11.9) be steel ball, the diameter of described steel ball is 1.5 ~ 2.5mm.
10. blood group serology automated detection system according to claim 8, is characterized in that, the hole size on described feeding chamber (11.1), movable push (11.2) and fixed head (11.4) all matches with the diameter of oscillating body.
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