CN103765221B - Blood group determination system - Google Patents
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- CN103765221B CN103765221B CN201180071227.XA CN201180071227A CN103765221B CN 103765221 B CN103765221 B CN 103765221B CN 201180071227 A CN201180071227 A CN 201180071227A CN 103765221 B CN103765221 B CN 103765221B
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N35/00009—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor provided with a sample supporting tape, e.g. with absorbent zones
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Abstract
The invention discloses a kind of blood group determination system, described system comprises: the consumed cylinder comprising polyester film, and wherein said polyester film has hole, in order to allow independent red blood cell through stopping aggegation blood compound simultaneously; Liquid adding portion, it is suitable for blood sample and reagent being added successively to described can consumption on cylinder; And being arranged on the described mechanical part that can consume above cylinder, it is suitable for the movement handling described liquid adding portion.The invention provides a kind of single system for blood group test.
Description
Technical field
The present invention relates to blood group test, and or rather, relate to a kind of system for testing rapidly blood group.
Background technology
Many blood relevant industries, such as, donate blood, transfuse blood, the raw material inspection of blood derived product etc., all require to test associated blood.Specifically, need to know whether there is relevant blood group to the heredity antigenic substance on red blood cell (RBC) surface.Hemagglutination, the RBC condensation namely when antibody exists, is typically used as biological phenomenon during blood group diagnosis.
Traditionally, blood group determination is accomplished manually.Blood sample/sample can mix with antibody by test operator on microslide, stirs the mixture, and waits for and cultivating, then examine under a microscope aggegation effect.But, aggegation be distinguished and UA RBC is very thorny, especially not be subject to trainer for what detect by an unaided eye.Therefore, propose multiple method to help aggegation effect is more easily found out by the mankind.The reliability of test result will depend on technology and the experience of test operator.In addition, loaded down with trivial details process makes it cannot meet the demand of fast large turnout, especially under the environment of blood bank and large hospital.
5th, 512, No. 432 U.S. patents disclose a kind of method distinguishing aggegation and non-aggegation RBC by means of inert gel easily.The blood sample of unknown blood group is placed in the reaction vessel of the potpourri being full of inert gel and antibody.Then centrifugally operated is carried out to this reaction vessel.If with the antigen that matches in RBC contained in blood sample, the RBC compound that so Ag-Ab produces after combining will be suspended on the upper surface of this inert gel.Otherwise this compound will be distributed in the centre or thereunder of inert gel.The defect of this method is two aspects: incubation time is long and centrifugally operated is too complicated.
5th, 759, No. 774 U.S. patents disclose the another kind of method using full-automatic microwell plate.Because the micropore on microwell plate has custom-designed shape, therefore, the blood of aggegation and UA blood can have different visual pattern after cultivation and mechanical vibration/centrifugally operated.Due to its standardized test process and high-throughput thereof, blood bank tends to the blood group determination method using robotization microwell plate as its standard.In order to meet high-throughput requirement, the matrix devising micropore in a microwell plate guarantees to carry out parallel testing.Therefore, the complexity of microwell plate and whole proving installation and cost all very high.In addition, the microwell plate of cleaning and recovery will reduce the overall efficiency in laboratory.
Summary of the invention
Need to provide a kind of good mode to carry out blood group test.One aspect of the present invention provides a kind of blood group determination system, and described system comprises: the consumed cylinder comprising polyester film, and wherein said polyester film has hole, in order to allow independent red blood cell through the blood compound stopping aggegation simultaneously; Liquid adding portion, it is suitable for blood sample and reagent being added successively to described can consumption on cylinder; And being arranged on the described mechanical part that can consume above cylinder, it is suitable for the movement handling described liquid adding portion.
Can find out, the invention provides a kind of simple system for blood group test.
Accompanying drawing explanation
Figure 1 shows that an embodiment of blood group determination system;
Figure 2 shows that the structure of the consumed cylinder according to one embodiment of the present invention;
Fig. 3 (a) to Fig. 3 (i) is depicted as the different possible signal combination stayed and can consume on cylinder;
Figure 4 shows that the example arrangement of the liquid suction tube for avoiding cross pollution;
Figure 5 shows that another embodiment of blood group determination system;
Figure 6 shows that the example arrangement of empty consumed cylinder;
Fig. 7 (a) to Fig. 7 (g) is depicted as different may the marking of staying during reverse blood group determination and can consume on cylinder.
In above-mentioned accompanying drawing, have and can consume cylinder 11, arrow 111, mechanical part 12, arrow 121, camera 13, blood sample interpolation module 14, liquid suction tube 141, pump 142, cleaning liquid interpolation module 15, liquid suction tube 151, pump 152, protect against biological hazards dustbin 16; Anti-A sub-segments 21, anti-B sub-segments 22, anti-D sub-segments 23, blank sub-segments 24, thieving paper 25; Capillary blood sampling transfer pipet 41, holder 42, pump 43, cylinder 44 can be consumed; Cylinder 51 can be consumed, mechanical part 52, camera 53, blood sample add module 54, liquid suction tube 541, pump 542, cleaning liquid add module 55, liquid suction tube 551, pump 552, protect against biological hazards dustbin 56, RBCC add module 57, liquid suction tube 571 and 573, pump 572 and 574; Polyester film 61, thieving paper 62; First sub-segments 701, second sub-segments 702, blank sub-segments 703.
Embodiment
The present invention provides a kind of blood group determination system in an exemplary embodiment, and described system comprises: the consumed cylinder comprising polyester film, and wherein said polyester film has hole, in order to allow independent red blood cell through stopping aggegation blood compound simultaneously; Liquid adding portion, it is suitable for blood sample and reagent being added successively to described can consumption on cylinder; And being arranged on the described mechanical part that can consume above cylinder, it is suitable for the movement handling described liquid adding portion.
Aggegation is the condensation between particulate.When aggegation related substances (as antibody or other aggegation related substanceses) exists, described aggegation related substances combines with multiple particulate, and adds them, thus forms the particle complex larger than independent particulate.Therefore, observe aggegation and can be used as qualification specific antigen and and then the method for qualification blood group.Because congealing reaction generation rate is very fast and be easy to produce, therefore, aggegation is a very important diagnostic techniques.The blood group proving installation proposed in one embodiment of the present invention or system are all based on RBC aggegation when there is aggegation related substances (as antibody or antigen).
Replace in gel and microwell plate, the blood group determination system proposed uses the custom-designed cylinder (such as, paper tape) that consumes to make aggegation result optics visible.That is, blood group can with the naked eye read from consuming cylinder.In addition, blood group determination system image capture module (such as, camera or charge-coupled device (CCD) (CCD)) catches image, and automatically analyzes this image, to determine the blood group dropping in the blood sample that can consume on cylinder.Specifically, blood group determination system can comprise with at least one in lower part: (1) can consume cylinder; (2) rolling portion, it can carry and described can consume cylinder and make it mobile; (3) multiple liquid suction tube, its respectively draw blood sample and reagent they are added to can consume on cylinder; (4) mechanical part, it handles the movement of liquid suction tube; (5) image capture module, it claps and gets the image that can consume cylinder after interpolation blood sample/sample and reagent; (6) image analysis module, its to analyze by image collection module clap the image got and generate final report.Liquid suction tube can be the hollow needle be made up of metal or plastics etc., and it comprises relevant pipe; Or can be the capillary pipet be made up of glass etc.In an exemplary application, liquid suction tube is disposable.
In one embodiment, can consume cylinder and comprise thieving paper and scribble the hollow polyester film/paper of antibody, described polyester film/paper bag draws together multiple section.Can be divided into each sub-segments can consume each section for single sample on cylinder, these sub-segments directly do not contact each other, to carry out different blood group tests.
Mechanical part guides liquid suction tube can consume on the polyester film of cylinder to collect blood sample and to be dropped in by this blood sample.The different antibodies be coated in the different sub-segments of polyester film is dissolved in blood sample, to treat next to react.Polyester film carries antibody and the reaction of RBC on the surface between antigen.If the antigen on the RBC of the antibody on polyester film and blood sample belongs to same blood group, so aggegation will be there is immediately between RBC.The average pore size of polyester film is about 50 microns, and wherein minimum-value aperture is 10 ~ 20 microns.In addition, the diameter of aggegation RBC compound is greater than 100 microns usually, and the size of single RBC is 10 microns or less.Therefore, when soaking polyester film with cleaning liquid, independent RBC successfully can move the hole by polyester film, but aggegation RBC compound then cannot pass through.In actual applications, the average pore size of polyester film can drop in the scope of 10 microns to 100 microns.
After in the sub-segments that blood sample is dripped to the polyester film that can consume cylinder, cleaning liquid is added to the same position/sub-segments place that can consume on cylinder by another liquid suction tube.After cleaning liquid contacts with thieving paper, the potpourri of thieving paper just absorbing blood sample and cleaning liquid, thus provide the single-way moving of liquid mixture is controlled.In other embodiments, polyester film can be surrounded by thieving paper or part encirclement completely.If there occurs aggegation, so RBC compound will be retained in polyester film, to indicate visual signal (such as, danger signal), this is because RBC compound is excessive, so that can not along with liquid mixture stream is together through the hole in polyester film.If there is not aggegation, so the movement along with liquid mixture is washed by RBC, thus makes polyester film not to leave any mark or only leave very shallow red-label (if any).Therefore, the known Antibody types be coated on polyester film, can judge according to the residual signal on polyester film, thus learns whether RBC exists specific antigen on the surface.This signal is finally got the image of polyester film by camera by bat and captures.Image analysis module is automatically analyzed image, and determines the blood group of blood sample.
Cross pollution between different sample, system uses disposable hollow pin or capillary pipet to collect blood sample and dripped to and can consume on cylinder, and is automatically thrown aside in the protect against biological hazards dustbin of specifying by hollow needle/capillary pipet after a procedure.
Specifically, Figure 1 shows that an embodiment of blood group determination system.It comprises with at least one in lower part: can consume cylinder 11; Mechanical part 12, it is for handling liquid suction tube 141 and 151 along arrow 121 direction or along the reciprocal movement of arrow 121; Camera 13; Blood sample adds module 14, it comprises liquid suction tube (such as, hollow needle or capillary pipet) 141 and pump 142, described liquid suction tube is for adding blood sample, and described pump is connected to make liquid suction tube 141 add blood sample with described liquid suction tube 141; And cleaning liquid adds module 15, it comprises liquid suction tube 151 and pump 152, and described liquid suction tube is for adding cleaning liquid, and described pump is connected to make liquid suction tube 151 add cleaning liquid with described liquid suction tube 151; Protect against biological hazards dustbin 16, it is for storing the hollow needle or capillary pipet used.In actual applications, mechanical part 12 and camera 13 can install (not shown in figure 1) on the support structure, and these two parts can be positioned at can consume cylinder 11 test zone above.In addition, blood group determination system comprises rolling portion, moves along arrow 111 direction or along the opposite direction of arrow 111 for making to consume cylinder 11; And the image analysis module to be connected with camera 13, these are in FIG and not shown.In this way, this blood group determination system can meet the demand of fast large turnout, especially under the environment of blood bank and large hospital.
Figure 2 shows that the structure of the consumed cylinder 11 according to Fig. 1 of one embodiment of the present invention.Cylinder 11 can be consumed and comprise two parts be made up of the different materials of two types: polyester film and thieving paper.Polyester film part scribbles different antibodies, and is attached to thieving paper part 25.The test zone of polyester film is divided into multiple section, and wherein each section has four sub-segments 21,22,23 and 24.Sub-segments 21,22 and 23 scribbles anti-A, anti-B and anti-D respectively.Sub-segments 24 is the blank sub-segments not scribbling antibody above, and it is used as reference.The layout of sub-segments 21-24 is not limited to shown in Fig. 2.Such as, sub-segments 21-24 can be respectively and not be coated with antibody, scribbles anti-A, anti-B and anti-D, or is respectively and scribbles anti-B, anti-D, anti-A, and is not coated with antibody.In addition, also there is less division part/gap and pass different sub-segments to avoid liquid flow.
After being dripped to by cleaning liquid on polyester film 21-24 and this cleaning liquid and thieving paper 25 comes in contact, thieving paper 25 absorbs the liquid mixture of cleaning liquid and blood sample.Therefore, which ensure that the single-way moving of liquid mixture in polyester film.
Should point out, adding blood sample to polyester film and adding after cleaning liquid subsequently, if there occurs RBC aggegation in blood sample, so polyester film will leave danger signal.The blood sample of different blood group will indicate unlike signal or unlike signal combination.
Fig. 3 (a) to Fig. 3 (i) is depicted as and stays the described different possible signal combination that can consume on cylinder 11.Pattern can be caught with camera 13 and for generating blood group determination result.Details row in Table 1, wherein+expression polyester film leave red color visible signal ,-represent that polyester film does not exist danger signal or only leaves very light signal.
| Anti-A | Anti-B | Anti-D | Contrast | Blood group | |
| Fig. 3 (a) | + | - | - | - | A,RH- |
| Fig. 3 (b) | - | + | - | - | B,RH- |
| Fig. 3 (c) | + | + | - | - | AB,RH- |
| Fig. 3 (d) | - | - | - | - | O,RH- |
| Fig. 3 (e) | + | - | + | - | A,RH+ |
| Fig. 3 (f) | - | + | + | - | B,RH+ |
| Fig. 3 (g) | + | + | + | - | AB,RH+ |
| Fig. 3 (h) | - | - | + | - | O,RH+ |
| Fig. 3 (i) | + or- | + or- | + or- | + | Test invalidation |
Table 1
Figure 4 shows that the example arrangement of the liquid suction tube for avoiding cross pollution.Specifically, liquid suction tube can be the capillary blood sampling transfer pipet for adding blood sample.First, operator collects blood sample with capillary blood sampling transfer pipet 41.After in the holder 42 capillary blood sampling transfer pipet 41 being loaded into mechanical part 12, capillary blood sampling transfer pipet 41 is moved to assigned address can consume above cylinder 44 by mechanical part 12, such as, can consume on a sub-segments of cylinder 44.Subsequently, the blood sample of a certain capacity is released by pump 43 from capillary blood sampling transfer pipet 41, can be consumed on cylinder 44 to drip to.After a test, capillary blood sampling transfer pipet 41 is dropped in dustbin by holder 42, and loads another capillary pipet and come to test next time.Holder 42 by such as clamping capillary blood sampling transfer pipet 41 or coupling with this capillary blood sampling transfer pipet 41 bayonet type, can fix capillary blood sampling transfer pipet 41.
In another embodiment, the reverse blood group determination of blood group determination system support provided.In this embodiment, with the addition of other liquid suction tube to system.These liquid suction tube are for dripping to can consume cylinder by red blood cell tester (RBCC).Compared with the consumed cylinder shown in Fig. 1 11, the polyester film of the consumed cylinder in Fig. 5 51 is blank and does not scribble antibody.First, the RBCC of different blood group adds in the different sub-segments of the polyester film that can consume cylinder 51 by system.The sub-segments being added different blood group RBCC can be regarded as the section of the blood group for the identification of single blood sample, although may not have special symbol to indicate this section can consume on cylinder 51.Subsequently, the same blood sample for testing is dripped to each sub-segments of different sub-segments by system.Reaction between polyester film carrying blood sample and RBCC.If the serum of blood sample contains associated antibodies, so the RBC aggegation of blood sample can occur in RBCC.Next, cleaning liquid adds to and can consume on cylinder by system.Similarly, aggegation blood compound will rest in the sub-segments of polyester film, thus indicates danger signal, and UA independent RBC will be washed.In addition, can by small refrigerator together with described system combination to preserve reagent, comprise RBCC, cleaning liquid etc.
Specifically, Figure 5 shows that another embodiment of blood group determination system, it supports reverse blood group determination.System shown in Fig. 5 comprises with at least one in lower part: blank/empty consumed cylinder 51; Mechanical part 52; Camera 53; Blood sample adds module 54, and it comprises liquid suction tube 541 and pump 542, and described liquid suction tube can consume cylinder 51 for collecting blood sample and being added to by this blood sample, and described pump is used for this blood sample to release liquid suction tube 541; Cleaning liquid adds module 55, and it comprises liquid suction tube 551 for adding cleaning liquid and pump 552; RBCC adds module 57, and it comprises liquid suction tube 571,573 for adding corresponding RBCC and pump 572,574; And protect against biological hazards dustbin 56, it is for reclaiming used liquid suction pipe, and described liquid suction tube can be such as hollow needle or capillary pipet.
Figure 6 shows that the example arrangement of empty consumed cylinder 51.Cylinder 51 can be consumed and comprise polyester film 61 for testing, and the thieving paper 62 directly contacted with this polyester film 61.Polyester film 61 is divided into multiple parallel sub-segments by less division part/gap, thus avoids liquid flow to pass these sub-segments.
Fig. 7 (a) to Fig. 7 (g) is depicted as different may the marking on the consumed cylinder 51 staying blank during reverse blood group determination, and wherein RBCC, blood sample and cleaning liquid are successively dripped to and can consume on cylinder 51.Specifically, the consumed cylinder shown in Fig. 7 (a) 51, wherein the first sub-segments 701 is added with RBCC-A, and the second sub-segments 702 is added with RBCC-B.Consumed cylinder shown in Fig. 7 (b) 51, wherein after interpolation RBCC, first sub-segments 701, second sub-segments 702 and blank sub-segments 703 add blood sample, and wherein said first sub-segments 701, second sub-segments 702 and blank sub-segments 703 form the section for testing blood sample jointly.Fig. 7 (c) to Fig. 7 (g) lists adding five kinds in the reverse blood group determination process after cleaning liquid different signal combination subsequently to these three sub-segments 701,702,703.Pattern is caught by camera 53 and blood group for determining blood sample.Details row in table 2, wherein+expression polyester film leave red color visible signal ,-represent that polyester film does not exist danger signal or only leaves very light signal.
| RBCC-A | RBCC-B | Contrast (Ctrl) | Blood group | |
| Fig. 7 (c) | + | + | - | O |
| Fig. 7 (d) | + | - | - | B |
| Fig. 7 (e) | - | + | - | A |
| Fig. 7 (f) | - | - | - | AB |
| Fig. 7 (g) | + or- | + or- | + | Test invalidation |
Table 2
Can find out, the invention discloses a kind of blood group determination system, what it use use aggegation related substances (such as, antibody or RBCC) coating/immersion has cost-benefit polyester film to replace gel column and microwell plate.In this way, invention removes cultivation and centrifugal process, thus reduce system complexity.In addition, standardization effort flow process greatly reduces mistake, thus ensure that the reliability of result.Specifically, the invention provides a kind of blood group determination system for detecting rapidly blood group, wherein said system automatically can test blood group, its mode is cooperating by liquid adding portion and mechanical part, thus the agglutinating reaction between antibody and antigen (if any) is occurred rapidly can consume on cylinder.More particularly, the present invention relates to a kind of system by testing blood group to the unlike signal analysis be associated with aggegation in various degree, wherein aggegation is that the described cylinder that consumes is custom-designed disposable material because the antigen on RBC surface is with to be coated in coexisting of the antibody that can consume on cylinder caused.In this way, present invention reduces the cost of blood group test.In addition, by saving cultivation and centrifugal process, it makes this process standardization, increases turnout and simplify system architecture.In addition, the invention provides the automatic analysis to aggegation result of the image utilizing camera to take.
Generally speaking, the present invention has at least one in following advantage.
1. standardisation process decreases mistake.
2. simple system architecture brings lower device manufacturing cost and maintenance cost.
The polyester film carrying that can consume cylinder is reacted and retains aggegation RBC compound (if having formation), thus shows visual signal, to pick out the blood group of tested blood sample.Do not need to accelerate its aggegation and its procedure for displaying with vibration in incubation.
3. test speed is fast.
The single-way moving of cleaning liquid in the porous structure of polyester film contributes to the contact between antibody and RBC surface antigen.Therefore, incubation time is for shorter antigen-antibody reaction.
4. can consume the low cost of manufacture of cylinder.
5. be coated in can consume on cylinder and be easy to preserve for the dry antibody of blood group determination.
6. more flexible.
Cylinder can be consumed can scribble antibody or not be coated with antibody.So, system can carry and the difference given expression to based on aggegation is tested.
7. during reverse blood group determination without the need to RBC and serum are separated.
8. because liquid suction tube can be disposable, therefore without the need to cleaning.
All features disclosed in this instructions (comprising any appended claims, summary and accompanying drawing), and/or any array configuration that can be combined in steps of disclosed method or process, except the array configuration that at least some in these features and/or step is repelled mutually.
Unless otherwise stated, each feature disclosed in this instructions (comprising any appended claims, summary and accompanying drawing) can be replaced with serving alternative features that is identical, equivalent or similar object.Therefore, unless otherwise stated, disclosed each feature is only the equivalence of a universal serial or an example of similar features.
Claims (12)
1. a blood group determination system, it comprises:
Comprise the consumed cylinder (11,44,51) of polyester film, wherein, described polyester film has hole, in order to allow independent red blood cell through stopping aggegation blood compound simultaneously; Described polyester film comprises multiple section, and each in described multiple section is for the identification of the blood group of single blood sample; Each in described multiple section comprises multiple sub-segments further, and described multiple sub-segments has gap each other and passes described sub-segments in order to avoid liquid flow, and in described multiple sub-segments at least two scribble dissimilar antibody;
Liquid adding portion, it is suitable for blood sample and reagent to add to successively and describedly can consumes on cylinder (11,44,51); And
Be arranged on the described mechanical part (12,52) that can consume above cylinder, it is suitable for the movement handling described liquid adding portion.
2. system according to claim 1, wherein said section comprises four sub-segments;
Three in described sub-segments scribble respectively anti-A, anti-B and anti-D (21,22,23) and last sub-segments be not coated with antibody (24).
3. system according to claim 1, wherein said liquid adding portion comprises:
Blood sample adds module (14), it comprises first liquid suction pipe (141,41) and pump (142,43), described first liquid suction pipe is for adding a sub-segments of described polyester film to by described blood sample, described pump is connected with described first liquid suction pipe and releases described first liquid suction pipe for by described blood sample;
Cleaning liquid adds module (15), it comprises second liquid suction pipe (151,41) and pump (152,43), described second liquid suction pipe is for adding the described sub-segments of described polyester film to by cleaning liquid, described pump is connected with described second liquid suction pipe and releases described second liquid suction pipe for by described cleaning liquid.
4. a blood group determination system, it comprises:
Comprise the consumed cylinder (11,44,51) of polyester film, wherein, described polyester film has hole, in order to allow independent red blood cell through stopping aggegation blood compound simultaneously; Described polyester film comprises multiple section, and each in described multiple section is for the identification of the blood group of single blood sample; Each in described multiple section comprises multiple sub-segments further, and described multiple sub-segments has gap each other and passes described sub-segments in order to avoid liquid flow, and described multiple sub-segments is not coated with antibody;
Liquid adding portion, it is suitable for blood sample and reagent to add to successively and describedly can consumes on cylinder (11,44,51); And
Be arranged on the described mechanical part (12,52) that can consume above cylinder, it is suitable for the movement handling described liquid adding portion.
5. system according to claim 4, wherein said liquid adding portion comprises:
Red blood cell tester adds module (57), it comprises multiple first liquid suction pipe (571,573,41) and pump (572,574,43), described multiple first liquid suction pipe is for adding the different sub-segments of described polyester film to by the red blood cell tester of different blood group, described pump is connected with corresponding first liquid suction pipe and releases described corresponding first liquid suction pipe for by described red blood cell tester;
Blood sample adds module (54), it comprises second liquid suction pipe (541,41) and pump (542,43), described second liquid suction pipe is for adding each in the described different sub-segments described polyester film with described red blood cell tester to by described blood sample, described pump is connected with described second liquid suction pipe and releases described second liquid suction pipe for by described blood sample; And
Cleaning liquid adds module (55), it comprises the 3rd liquid suction tube (551,41) and pump (552,43), described 3rd liquid suction tube is for adding each of described different sub-segments to by cleaning liquid, described pump is connected for described cleaning liquid being released described 3rd liquid suction tube with described 3rd liquid suction tube.
6. the system according to claim 3 or 5, wherein said liquid suction tube is hollow needle or capillary pipet.
7. the system according to claim 3 or 5, wherein said mechanical part (12,52) comprises multiple holder (42), described liquid suction tube for loading liquid suction tube, and is dropped in dustbin (16,56) by described multiple holder after a procedure.
8. the system according to any one of claim 1 to 5, it comprises further:
Be arranged on the described image capture module (13,53) that can consume cylinder (11,44,51) top, it is suitable for after adding described blood sample and reagent, take the described image that can consume cylinder.
9. system according to claim 8, wherein said mechanical part (12,52) and described image capture module (13,53) are installed in supporting construction.
10. system according to claim 8, it comprises further: image analysis module, its to be suitable for described image capture module (13,53) clap the described image got and analyze and generate blood group determination result.
11. systems according to any one of claim 1 to 5, the wherein said cylinder (11,44,51) that consumes comprises the thieving paper (25,62) contacted with described polyester film further, and described thieving paper is used for the potpourri of described blood sample and described reagent to sponge from described polyester film.
12. systems according to any one of claim 1 to 5, it comprises further: rolling portion, and it is suitable for can consuming cylinder (11,44,51) described in carrying and moving it.
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| PCT/CN2011/074728 WO2012159275A1 (en) | 2011-05-26 | 2011-05-26 | Blood typing system |
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| CN103765221A CN103765221A (en) | 2014-04-30 |
| CN103765221B true CN103765221B (en) | 2015-12-09 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN201180071227.XA Expired - Fee Related CN103765221B (en) | 2011-05-26 | 2011-05-26 | Blood group determination system |
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| WO (1) | WO2012159275A1 (en) |
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| EP3171178A1 (en) * | 2015-11-23 | 2017-05-24 | F. Hoffmann-La Roche AG | System and method for preparing a biological sample for analysis |
| CN106290922B (en) * | 2016-09-20 | 2018-04-10 | 刘大基 | ABO is positive and negative and RhD antigens bloodtypes identify combiner and authentication method |
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| CN101782588B (en) * | 2010-01-27 | 2012-10-03 | 苏州生物医学工程技术研究所 | Double-needle sample sucking mechanism applicable to full-automatic blood type analytical instrument |
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- 2011-05-26 WO PCT/CN2011/074728 patent/WO2012159275A1/en active Application Filing
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| US3624223A (en) * | 1964-12-17 | 1971-11-30 | Technicon Instr | Blood type indicator |
| GB2250342A (en) * | 1990-11-27 | 1992-06-03 | Pall Corp | Blood typing |
| CN1894584A (en) * | 2003-07-09 | 2007-01-10 | 麦迪奥诊断产品有限公司 | Device and method for simultaneously carrying out blood group determination, serum cross-check and antibody detection test |
| CN101603967A (en) * | 2008-06-10 | 2009-12-16 | 英科新创(厦门)科技有限公司 | The method and the kit of fast detecting people ABO/Rh/MN blood group |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106814199A (en) * | 2017-03-27 | 2017-06-09 | 武汉优视科技有限公司 | A kind of automatic checkout system of Western blotting |
| CN106814199B (en) * | 2017-03-27 | 2019-01-29 | 武汉优视科技有限公司 | A kind of automatic checkout system of immunoblotting |
Also Published As
| Publication number | Publication date |
|---|---|
| CN103765221A (en) | 2014-04-30 |
| WO2012159275A1 (en) | 2012-11-29 |
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