CN104789546B - 一种脱乙酰基酶突变体及其应用 - Google Patents
一种脱乙酰基酶突变体及其应用 Download PDFInfo
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- CN104789546B CN104789546B CN201510057199.6A CN201510057199A CN104789546B CN 104789546 B CN104789546 B CN 104789546B CN 201510057199 A CN201510057199 A CN 201510057199A CN 104789546 B CN104789546 B CN 104789546B
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- acetylglucosamine
- escherichia coli
- enzyme
- udp
- ala
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/78—Hydrolases (3) acting on carbon to nitrogen bonds other than peptide bonds (3.5)
- C12N9/80—Hydrolases (3) acting on carbon to nitrogen bonds other than peptide bonds (3.5) acting on amide bonds in linear amides (3.5.1)
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/26—Preparation of nitrogen-containing carbohydrates
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y305/00—Hydrolases acting on carbon-nitrogen bonds, other than peptide bonds (3.5)
- C12Y305/01—Hydrolases acting on carbon-nitrogen bonds, other than peptide bonds (3.5) in linear amides (3.5.1)
- C12Y305/01108—UDP-3-O-acyl-N-acetylglucosamine deacetylase (3.5.1.108)
Abstract
Description
反应时间 | D-氨基葡萄糖(g/L) | 转化率(%) |
1 | 29.6 g/L | 43.0% |
2 | 62.8 g/L | 91.3% |
3 | 69.8 g/L | 101.5% |
2 | 68.2 g/L | 99.1% |
3 | 69.1 g/L | 100.4% |
Claims (9)
Priority Applications (1)
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CN201510057199.6A CN104789546B (zh) | 2015-02-04 | 2015-02-04 | 一种脱乙酰基酶突变体及其应用 |
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CN201510057199.6A CN104789546B (zh) | 2015-02-04 | 2015-02-04 | 一种脱乙酰基酶突变体及其应用 |
Publications (2)
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CN104789546A CN104789546A (zh) | 2015-07-22 |
CN104789546B true CN104789546B (zh) | 2016-08-17 |
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CN201510057199.6A Expired - Fee Related CN104789546B (zh) | 2015-02-04 | 2015-02-04 | 一种脱乙酰基酶突变体及其应用 |
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Families Citing this family (3)
Publication number | Priority date | Publication date | Assignee | Title |
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CN114014944B (zh) * | 2021-11-05 | 2022-08-26 | 南开大学 | 一种天然合成的低酰基三赞胶及其生产方法和鉴定方法 |
CN114672522B (zh) * | 2022-04-08 | 2023-12-26 | 山东大学 | 一种双酶催化n-乙酰-d-葡萄糖酸内酯生产2-酮基-3-脱氧-d-葡萄糖酸的方法 |
CN114836361B (zh) * | 2022-04-11 | 2023-09-12 | 扬州日兴生物科技股份有限公司 | 一种高产氨基葡萄糖的解淀粉芽孢杆菌工程菌株及其应用 |
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CN104789546A (zh) | 2015-07-22 |
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Effective date of registration: 20190823 Address after: 210018 No. 11 Netjie City, Xuanwu District, Nanjing City, Jiangsu Province Patentee after: Sun Jingfang Address before: 243100 Xinqiao Industrial Concentration Zone, Dangtu County, Ma'anshan City, Anhui Province Patentee before: Square bio tech ltd, Anhui |
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Effective date of registration: 20200511 Address after: 236000 No.82 zhoupeng Road, Fuyang Circular Economy Park, Yingquan District, Fuyang City, Anhui Province Patentee after: Anhui Fufang high tech Biotechnology Co., Ltd Address before: 210018 No. 11 Netjie City, Xuanwu District, Nanjing City, Jiangsu Province Patentee before: Sun Jingfang |
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