CN104771463B - New application of the european bird cherry extract in alcoholic liver injury protection medicine and health products are prepared - Google Patents
New application of the european bird cherry extract in alcoholic liver injury protection medicine and health products are prepared Download PDFInfo
- Publication number
- CN104771463B CN104771463B CN201510165354.6A CN201510165354A CN104771463B CN 104771463 B CN104771463 B CN 104771463B CN 201510165354 A CN201510165354 A CN 201510165354A CN 104771463 B CN104771463 B CN 104771463B
- Authority
- CN
- China
- Prior art keywords
- bird cherry
- european bird
- extract
- health products
- european
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Landscapes
- Medicines Containing Plant Substances (AREA)
- Medicinal Preparation (AREA)
Abstract
The invention belongs to pharmaceutical technology field, essentially discloses new application of the european bird cherry extract in alcoholic liver injury protection medicine and health products are prepared.Experimental researches prove that：The european bird cherry extract has significant protective effect to alcohol induced liver injury mouse, can effectively reduce ALT in mice serum, AST and TG level, raises GSH levels in hepatic tissue, it is horizontal to reduce lipid peroxidation product MDA.Therefore, it is possible to use the extract prepares liver-protecting medicine, health products, food additives or compound preparation etc..Its medicine or health products can be existing any formulations, such as tablet, capsule, powder-injection, injection, pill, soft capsule, granule and patch etc..
Description
Technical field
The present invention relates to new application of the european bird cherry extract in liver injury protection medicine and health products are prepared, belong to Chinese medicine and carry
Take the pharmaceutical technology field of the new activity of thing.
Background technology
Chemical damage is a kind of common liver disease, mainly caused by the series damage caused by oxidative stress, such as weight
Depending on inadequate, hepatitis, liver fibrosis, hepatonecrosis, or even hepatic sclerosis and liver cancer are easily evolved into, Medical bound pair is in chemically liver
Damage no preferable therapeutic strategy.However, a variety of active ingredients found from Chinese medicine has obvious prevention and treatment liver
The effect of damage.Therefore, find and find with prevention and treatment chemical damage preparation with certain from natural drug
Theory significance and realistic meaning.
European bird cherry platymiscium is various in China's species, is perennial arbor, is planted extensively in China, because cold-resistant shade tolerant is in northeast
Area growth is intensive.Mainly include mountain peach european bird cherry（Padus maackii）, star hair european bird cherry（Padus buergeriana）, european bird cherry
（Padus racemosa）, brown hair european bird cherry（Padus brunnescens）And purple leaf european bird cherry（Padus virginiana）Deng.
It is a kind of important to view and admire species in China because the platymiscium is overall beautiful.Wherein mountain peach european bird cherry, european bird cherry and purple leaf european bird cherry are rose
Common vetch section cherry, it is distributed more widely in northeast.
Research shows：The leaf of european bird cherry platymiscium can be used for making product（Bird cherry leaf tea and preparation method thereof, patent,
ZL. 200610010198.7）, flower containing substantial amounts of benzaldehyde because that can do anti-corrosion materials（Zhao Qiuyan, european bird cherryPrunus padusLinn. volatile oil chemical composition analysis,《Plant research》, 2003）, in addition, the abundant flavones ingredient that european bird cherry contains
（He Yingxia, the optimal extraction technology of flavones in purple leaf european bird cherry blade《Northern gardening》, 2013）And anthocyanin type components（It is pungent
More, european bird cherry belongs to three kinds of fruit anthocyanin antioxidant activity researchs, Northeast Forestry University's Master's thesis, 2013）.Above-mentioned document report
Road anti-oxidant and to HepG2 the cytotoxic activity of european bird cherry（Cold plum, the antioxidation activity of purple leaf european bird cherry fruit anthocyanin, food
Product science, 2013；Xin Yue, european bird cherry belong to three kinds of fruit anthocyanin and the inhibited proliferation of HepG2 cells are compared, food industry
Science and technology, 2013）.
Preparation method progress patent of the applicant to european bird cherry extract discloses a kind of " preparation side of european bird cherry extract
Method and the application in reducing blood lipid medicine and health products "（CN：104367659A）, the present invention is on the basis of published patent
And the new bioactivity of the new discovery extract, belong to and report that european bird cherry extract causes chemical damage to have alcohol first
Protective effect, and can be used in preparing the application in medicine and health products.In short, present invention novelty proposes european bird cherry extraction
Application of the thing in alcoholic liver injury protection medicine and health products are prepared, it is found that the extract is expected to exploitation and is used for wine into one kind
The medicine or health product raw material of smart liver injury protection.
The content of the invention
The present invention provides medicine or health products of a kind of european bird cherry extract in terms of chemical damage is prevented and treated
And the application of food additives.
According to a kind of disclosed " preparation method of european bird cherry extract and in reducing blood lipid medicine and health products of inventor
In application "（CN：104367659A）Patent application, the preparation method of european bird cherry extract have following feature：European bird cherry extract
Obtain as follows：Dry european bird cherry sample, crushed 20 mesh sieves, through appropriate solvent and extracting method, be extracted
Liquid, is concentrated under reduced pressure into dry；No precipitation, upper macroporous adsorbent resin column chromatography, successively with water and more than 70% ethanol are suspended to water
Eluted, alcohol elution fraction is collected, up to european bird cherry extract.
Foregoing european bird cherry, selected from mountain peach european bird cherry（Padus maackii）, european bird cherry（Padus racemosa）Or purple leaf european bird cherry
（Padus virginiana）Any type；Extract part is selected from its fruit, any position such as cauline leaf or flower, its preferred fructo
It is real.Extracting method is selected from any type of soak extraction, refluxing extraction, ultrasonic extraction, adverse current extraction, supercritical extract etc.；Carry
Solvent is taken to be selected from water, methanol, ethanol any type and different proportion mixture；Macroporous absorption for the processing of sample subsequent purification
Column chromatography, including it is pharmaceutically acceptable, it can be used for separated a variety of usual resins, selected from D101, NKA12 or ADS etc.
Any type.
Foregoing european bird cherry extract, mainly comprising antioxidant contents such as flavones, anthocyanin.
Foregoing european bird cherry extract, has Hepatocyte protection, can be used for answering in medicine, health products and food additives
With.
When the present invention is used for such use, it is administered orally or parenterally safe, in the case of oral, its
Can by it is any it is conventional in the form of be administered, such as tablet, capsule, powder-injection, injection, pill, soft capsule, granule and patch
Deng.
The present invention prepares the european bird cherry extract with Hepatocyte protection and can be mixed with pharmaceutical carrier or food carriers, here
The solid or the excipient of liquid used is well known in the art, and is given some instances below, and powder is powder for oral administration
Agent, its excipient have lactose, starch, dextrin, calcium carbonate, synthesis or puritan filler aluminium, magnesia, magnesium stearate, bicarbonate
Sodium, dry yeast etc.；The excipient of solution has water, glycerine, 1,2-PD, simple syrup, ethanol, ethylene glycol, polyethylene glycol,
D-sorbite etc.；The excipient of ointment can use fatty oil, agnolin, vaseline, glycerine, beeswax, haze tallow, liquid stone
The water-repelling agent or hydrophilizing agent that wax etc. is combined into.
The present invention can be further illustrated by following experimental example.
Protective effect of the experimental example european bird cherries extract to alcohol-induced acute hepatic injury mice
1 material and method
1.1 instrument
BP211D assay balances（Shanghai Shu Xin finite instruments company）；HC-2517 supercentrifuges（Anhui Zhong Kezhongjia sections
Learn Instrument Ltd.）；High-speed homogenizer（Weifang San Shui examines equipment Co., Ltd）；Continuous spectrum scan-type microplate reader
SpectraMaxPlus384（Molecular biosciences instrument company of the U.S.）；The full-automatic biological tissue embedding machines of YD-6D, paraffin slicing machine
（Yi Di Medical Equipment Plants of Jinhua, Zhejiang Province city）.
Material and reagent
European bird cherry extract, from purple leaf european bird cherry（Padus virginiana）Fruit, preparation method is with reference to disclosing
Patent " a kind of preparation method of european bird cherry extract and the application in reducing blood lipid medicine and health products "（CN：104367659A）
Ethanol, Beijing Chemical Plant；Alanine aminotransferase（ALT）, aspartate amino transferase（AST）, the third two
Aldehyde（MDA）, superoxide dismutase（SOD）Bioengineering Research Institute is built up in assay kit, Nanjing；Protein testing cassete, north
The safe clinical reagent Co., Ltd of Jing Beiization；Triglyceride reagent box is purchased from safe clinical reagent Co., Ltd of Beijing North China.
Experimental animal
SPF grades of ICR mouse, male, weight 20-22g, purchased from this experimental animal technology Co., Ltd of Changchun hundred million,
Quality certification number：SCXK（It is lucky）2011-0004, adaptability are tested after raising 1 week.
Adaptability feed 1 week after male ICR mouse, be randomly divided into 6 groups, i.e. blank control group, model group, silymarin
Positive controls（150 mg·kg-1）, european bird cherry extract low dose group（100mg·kg-1）, middle dose group（200mg·kg-1）
With high dose group（400mg·kg-1）.Positive controls and administration group use gastric infusion, continuous 7 days, blank control group and mould
Type group gives isometric physiological saline, and after 1 h of last dose, in addition to blank group, remaining each group disposably gives volume fraction
For 50% alcohol, by 4.8gkg-1(12mL/kgbw) inducing acute alcoholic liver injury, fasting for solids but not liquids.6 it is small when after eye
Socket of the eye veniplex is taken a blood sample, separation serum, and after 12h, cervical dislocation puts to death mouse, quickly removes each group liver and spleen, weighs, and calculates liver and refers to
Number and spleen index (internal organs weight/weight × 100%).
Experimental result
The influence of 2.1 pairs of mouse weights and organ index
As it can be seen from table 1 after being administered 7 days, each group the weight of animals increased, but each administration group and blank control group
Compared to weight without significant difference (P＞ 0.05), show that european bird cherry extract does not influence mouse weight.Research shows：Disposably
Heavy dose of alcohol impact can cause the significantly raised of mice organs index, mainly include liver index and spleen index.This experiment is tied
Fruit shows：Compared with blank group, model group mouse liver index and spleen index dramatically increase (P＜ 0.05), and with administration 7 days after,
Two administration groups of european bird cherry extract and silymarin administration group can degree reduction liver index and spleen index (P＜
0.05), as a result such as following table.
Influence of the 1 european bird cherry extract of table to mouse weight and Immune Organs Index（,n=8）
" * " model group is compared with blank control group, and " # " administration group is compared with model group, significant difference（P<0.05）.
The influence of ALT, AST and TG level in 2.2 pairs of mice serums
From table 2 it can be seen that compared with blank control group, the level of ALT, AST and TG of model group substantially increase, and say
Bright acute alcohol induced liver injury model creation success（P<0.05）；Compared with model group, various dose european bird cherry extract is given
（200 and 400mg/kg）Each group mice serum in ALT, AST and TG content substantially reduce, there is statistical significance（P<
0.05）, and ALT and AST is on close level the level of positive controls in high dose group.The result shows that：European bird cherry extract energy
The rise of enough different degrees of suppression acute alcohol hepatic injury mouse ALT, AST and TG levels, as a result such as following table.
Influence of the 2 european bird cherry extract of table to mice serum ALT, AST and TG content（,n=8）
" * " model group is compared with blank control group, and " # " administration group is compared with model group, significant difference（P<0.05）.
The influence of MDA and GSH levels in 2.3 pairs of murine liver tissues
From table 3 it can be seen that compared with blank control group, the MDA levels of model group are significantly raised, and GSH contents are shown
Write and decline, illustrate that disposable heavy dose of alcohol impact can cause oxidativestress damage, show acute alcohol induced liver injury mould
Type is successfully established（P<0.05）；Compared with model group, the MDA water of two dosage group mouse of european bird cherry extract 200 and 400mg/kg
It is flat gradually to be reduced with the increase of dosage, and have significant difference（P<0.05）；And the content of GSH is with giving european bird cherry
The increase of extract doses has been lifted and has been in certain dose relationship（P<0.05）.The result shows that：European bird cherry extract can
The oxidativestress damage of acute hepatic injury mice generation is effectively improved, as a result such as following table.
Influence of the 3 european bird cherry extract of table to mouse liver MDA and GSH（,n=8）
" * " model group is compared with blank control group, and " # " administration group is compared with model group, significant difference（P<0.05）.
2.4 pairs of murine liver tissue pathology section examinations
Pathology of hepar is observed as shown in the figure, normal mouse liver histocyte marshalling, nuclear structures and
Phenomena such as cell boundaries are clear, cell rope aligned orderly, fat-free degeneration necrosis, no inflammation cellular infiltration；The visible liver of model group
Iuntercellular boundary obscures, and central vein expands, and occurs inflammatory cell infiltration and a large amount of denaturation vacuoles, part of hepatocytes core into the cell
Phenomena such as atrophy or disappearance；Compared with model group, milk thistle positive controls hepatic injury has a significant mitigation, liver cell structure and
Liver rope is arranged substantially close to normal, no inflammatory cell infiltration and lipid vacuole；After european bird cherry extract for treating is given 7 days, mouse
In liver cell, it can clearly be seen that swelling and cracking degree mitigate, inflammatory cell infiltration and denaturation vacuole are reduced, but still visible
Part apoptotic cell and denaturation vacuole, figure omit
Embodiment prepared by embodiment medicine and health products
Prepare the embodiment one of medicament
The preparation of capsule
European bird cherry extract 200g, appropriate medical starch, both fully mix, encapsulated, and 1000 capsules, every weight is made
0.25g, every 200mg of extract containing european bird cherry.It is oral, 4 tablets each time, three times a day.
Prepare the embodiment two of medicament
The preparation european bird cherry extract 200g of tablet, appropriate medical starch, both fully mix, and adhesive system is done with ethanol
Wet granular, it is dry, 120 mesh sieve whole grains are crossed, encapsulated, every 200 mg are 1-2 oral every time, 2 times a day.
Prepare the embodiment three of medicament
Pill prepares polyethylene glycol4000300g, melts in water-bath, adds european bird cherry extraction raw material 200g, stirs
Mix uniformly, be poured into insulating tube, regulating thermostatic device, makes liquid be instilled at 80-90 DEG C in cooled atoleine（Temperature
±4℃）, after dripping off, pill is poured on filter paper and blots paraffin oil, add a small amount of talcum powder, mixed, obtain european bird cherry extract drop
1000, ball.It is oral, one time 4, three times a day, one after each meal.
In addition to the above embodiment, other equivalent technical solutions should also be as within its protection domain the present invention,
This is no longer described one by one.
Claims (3)
1. new application of the european bird cherry extract in treatment alcoholic liver injury protection medicine and health products are prepared, european bird cherry extract lead to
Cross following steps acquisition：Dry european bird cherry sample, crushed 20 mesh sieves, through appropriate solvent and extracting method, obtain extracting solution,
It is concentrated under reduced pressure into dry；Be suspended to no precipitation with water, upper macroporous adsorbent resin column chromatography, successively with water and more than 70% ethanol into
Row elution, collects alcohol elution fraction, and extract mainly includes flavones, anthocyanin, and the european bird cherry is selected from mountain peach european bird cherryPadus maacki, european bird cherryPadus racemosaOr purple leaf european bird cherryPadus virginiana, liver cell is swollen after giving european bird cherry extract
Swollen and cracking degree mitigates, and inflammatory cell infiltration and denaturation vacuole are reduced.
2. purposes according to claim 1, it is characterised in that the hepatic injury refers to alcohol-induced acute liver damage
Mouse model.
3. purposes according to claim 1, it is characterised in that the medicine or health products are tablets, capsule, powder-injection,
Injection, pill, soft capsule, granule and patch.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510165354.6A CN104771463B (en) | 2015-04-09 | 2015-04-09 | New application of the european bird cherry extract in alcoholic liver injury protection medicine and health products are prepared |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510165354.6A CN104771463B (en) | 2015-04-09 | 2015-04-09 | New application of the european bird cherry extract in alcoholic liver injury protection medicine and health products are prepared |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN104771463A CN104771463A (en) | 2015-07-15 |
| CN104771463B true CN104771463B (en) | 2018-04-27 |
Family
ID=53613448
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201510165354.6A Active CN104771463B (en) | 2015-04-09 | 2015-04-09 | New application of the european bird cherry extract in alcoholic liver injury protection medicine and health products are prepared |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN104771463B (en) |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108815258B (en) * | 2018-08-28 | 2021-10-08 | 西安交通大学 | Amur elsholtzia extract and preparation method and application thereof |
-
2015
- 2015-04-09 CN CN201510165354.6A patent/CN104771463B/en active Active
Non-Patent Citations (2)
| Title |
|---|
| 山桃稠李果实花色苷对HepG2 细胞抗氧化系统的影响;刘荣等，;《食品工业科技》;20131231;第34卷(第15期);第66-69页 * |
| 稠李属果实色素梨花性质抗氧化抗疲劳作用研究;任健;《万方数据库》;20120428;第1、5-10、12-13、41-50页 * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN104771463A (en) | 2015-07-15 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| KR101640258B1 (en) | Anti-obesity composition comprising red grape extracts, green tea extracts, soybean extracts, and L-carnitine | |
| KR102189416B1 (en) | Composition for preventing obesity and reducing body fat comprising complex extracts of Inula japonica | |
| CN109200136A (en) | A kind of Chinese medicine composition and its application for Antialcoholic liver-protecting | |
| CN114028533A (en) | Composition with functions of dispelling effects of alcohol and protecting liver and preparation method and application thereof | |
| KR101794006B1 (en) | Anti inflammatory comprising plant extract | |
| CN104771463B (en) | New application of the european bird cherry extract in alcoholic liver injury protection medicine and health products are prepared | |
| KR101282708B1 (en) | Composition for Anti-Obesity or Reducing Body Fat, Containg MeJA-Treated Buckwheat Sprout | |
| CN115444922B (en) | Anti-fatigue medicinal and edible composition and application thereof | |
| CN104288164B (en) | Pharmaceutical composition for preventing acute alcoholic liver injury | |
| CN113164541A (en) | Composition for preventing, treating or improving male climacteric syndrome containing Sambucus nigra extract as active ingredient | |
| KR20160011846A (en) | Anti-obesity composition containing bitter melon mixture hot water extract and manufacturing method thereof | |
| CN105749072B (en) | Chinese medicinal composition for preventing and treating hyperuricemia and hyperlipemia and preparation method thereof | |
| CN108785476A (en) | The preparation method and application of solid composite containing A Sayi fruits and Herba Cistanches | |
| CN103784492B (en) | Common pratia herb extract and its application with antitumor action | |
| CN113350390A (en) | Total sesquiterpene lactone of saussurea involucrata, preparation method and pharmaceutical application thereof | |
| CN110882319A (en) | Application of thoroughfare bitter orange, thoroughfare bitter orange extract and products containing thoroughfare bitter orange extract in preventing and/or treating metabolic liver diseases | |
| CN106474322A (en) | Black garlic polyphenol oral liquid containing Eurycoma longifolia extract product and preparation method thereof | |
| Shah et al. | A systemic phytopharmacological review of multipotential medicinal plant milk thistle | |
| KR101181347B1 (en) | Composition for the prevention and treatment of lipid-related cardiovascular disease or obesity containing the extracts of Dictamnus dasycarpus as active ingredient | |
| CN111632082A (en) | American ginseng and hovenia dulcis thunb composition and application thereof in preparing medicine for preventing and treating alcoholic liver injury | |
| CN104473992B (en) | A kind of Radix Platycodonis extract and the new application in medicine and health products are prepared | |
| CN112494626B (en) | Tablet with protective effect on chemical liver injury and preparation method thereof | |
| CN110840950A (en) | Application of Russian tea and/or Russian tea extract in preparation of medicines for preventing and treating non-alcoholic liver disease and/or non-alcoholic liver injury | |
| CN106975014B (en) | Preparation method and application of ginseng fruit anthocyanin | |
| CN103623109A (en) | Traditional Chinese medicine composition for treating ischemic cerebrovascular disease and preparation method thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| EXSB | Decision made by sipo to initiate substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| CB03 | Change of inventor or designer information |
Inventor after: Li Wei Inventor after: Zhang Yang Inventor after: Ye Qiang Inventor after: Zhang Jing Inventor after: Lin Hongmei Inventor after: Wang Zi Inventor after: Mao Xinxin Inventor after: Li Huiping Inventor after: Wang Yingping Inventor before: Li Wei Inventor before: Zhang Jing Inventor before: Lin Hongmei Inventor before: Wang Zi Inventor before: Mao Xinxin Inventor before: Li Huiping Inventor before: Wang Yingping |
|
| CB03 | Change of inventor or designer information | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |