CN104769130A - 一种确定rna完整性的方法 - Google Patents

一种确定rna完整性的方法 Download PDF

Info

Publication number
CN104769130A
CN104769130A CN201380029890.2A CN201380029890A CN104769130A CN 104769130 A CN104769130 A CN 104769130A CN 201380029890 A CN201380029890 A CN 201380029890A CN 104769130 A CN104769130 A CN 104769130A
Authority
CN
China
Prior art keywords
rna
sample
integrity
rna molecule
entity
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CN201380029890.2A
Other languages
English (en)
Chinese (zh)
Inventor
亚历山大·艾伦·莫利
迈克尔·朱利安·布里斯科
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Monoquant Pty Ltd
Original Assignee
Monoquant Pty Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Monoquant Pty Ltd filed Critical Monoquant Pty Ltd
Publication of CN104769130A publication Critical patent/CN104769130A/zh
Pending legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G16—INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
    • G16B—BIOINFORMATICS, i.e. INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR GENETIC OR PROTEIN-RELATED DATA PROCESSING IN COMPUTATIONAL MOLECULAR BIOLOGY
    • G16B25/00—ICT specially adapted for hybridisation; ICT specially adapted for gene or protein expression
    • G16B25/10—Gene or protein expression profiling; Expression-ratio estimation or normalisation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6851—Quantitative amplification
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6809—Methods for determination or identification of nucleic acids involving differential detection
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6883—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material
    • C12Q1/6886—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material for cancer
    • G—PHYSICS
    • G16—INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
    • G16B—BIOINFORMATICS, i.e. INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR GENETIC OR PROTEIN-RELATED DATA PROCESSING IN COMPUTATIONAL MOLECULAR BIOLOGY
    • G16B25/00—ICT specially adapted for hybridisation; ICT specially adapted for gene or protein expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2537/00—Reactions characterised by the reaction format or use of a specific feature
    • C12Q2537/10—Reactions characterised by the reaction format or use of a specific feature the purpose or use of
    • C12Q2537/165—Mathematical modelling, e.g. logarithm, ratio
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/158—Expression markers
    • G—PHYSICS
    • G16—INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
    • G16B—BIOINFORMATICS, i.e. INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR GENETIC OR PROTEIN-RELATED DATA PROCESSING IN COMPUTATIONAL MOLECULAR BIOLOGY
    • G16B99/00—Subject matter not provided for in other groups of this subclass

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Physics & Mathematics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biophysics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Evolutionary Biology (AREA)
  • Theoretical Computer Science (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Spectroscopy & Molecular Physics (AREA)
  • Medical Informatics (AREA)
  • Pathology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Oncology (AREA)
  • Hospice & Palliative Care (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
CN201380029890.2A 2012-04-23 2013-04-23 一种确定rna完整性的方法 Pending CN104769130A (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201261637253P 2012-04-23 2012-04-23
US61/637,253 2012-04-23
PCT/AU2013/000422 WO2013159145A1 (en) 2012-04-23 2013-04-23 A method of determining rna integrity

Publications (1)

Publication Number Publication Date
CN104769130A true CN104769130A (zh) 2015-07-08

Family

ID=49482030

Family Applications (1)

Application Number Title Priority Date Filing Date
CN201380029890.2A Pending CN104769130A (zh) 2012-04-23 2013-04-23 一种确定rna完整性的方法

Country Status (6)

Country Link
US (1) US9411929B2 (https=)
EP (1) EP2841602B1 (https=)
JP (1) JP6262203B2 (https=)
KR (1) KR102068272B1 (https=)
CN (1) CN104769130A (https=)
WO (1) WO2013159145A1 (https=)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104769130A (zh) 2012-04-23 2015-07-08 莫诺夸特有限公司 一种确定rna完整性的方法
US9422602B2 (en) * 2012-08-15 2016-08-23 Bio-Rad Laboratories, Inc. Methods and compositions for determining nucleic acid degradation
BR112018017118A2 (pt) 2016-02-22 2019-03-06 Toray Industries método e programa(s) de avaliação da qualidade de mirna, meio de gravação e chip para avaliação e análise da qualidade de mirna
CN112041933B (zh) * 2018-03-14 2025-05-13 皇家飞利浦有限公司 使用局部独特特征来解释rna测序数据的转录本表达水平的系统和方法
US20210301340A1 (en) 2018-07-31 2021-09-30 Toray Industries, Inc. Method of evaluating quality of body fluid specimen
JP2022532170A (ja) * 2019-05-10 2022-07-13 インテグレーテッド ナノ－テクノロジーズ，インコーポレイティド Ｒｎａ転写物の分析システム及び方法

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1997002361A1 (en) 1995-07-03 1997-01-23 Akzo Nobel N.V. A method for determining the integrity of nucleic acid
ATE331044T1 (de) 2003-03-04 2006-07-15 Pamgene Bv Verfahren zur integrierten integritätsbewertung und analyse von nukleinsäuren
US20060063170A1 (en) 2004-03-18 2006-03-23 Arcturus Bioscience, Inc. Determination of RNA quality
US9150909B2 (en) 2008-08-29 2015-10-06 Bio-Rad Laboratories, Inc. Determination of the integrity of RNA
CN104769130A (zh) 2012-04-23 2015-07-08 莫诺夸特有限公司 一种确定rna完整性的方法

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
ALEXANDRE HO-PUN-CHEUNG等: "Reverse transcription-quantitative polymerase chain reaction:description of a RIN-based algorithm for accurate data normalization", 《BMC MOLECULAR BIOLOGY》 *
MICHAEL J. BRISCO等: "Incorporation of measurement of DNA integrity into qPCR assays", 《BIOTECHNIQUES》 *
XIN GONG等: "Quantification of RNA damage by reverse transcription polymerase chain reactions", 《ANALYTICAL BIOCHEMISTRY》 *

Also Published As

Publication number Publication date
EP2841602B1 (en) 2017-06-21
KR20150014937A (ko) 2015-02-09
KR102068272B1 (ko) 2020-01-20
US20130309680A1 (en) 2013-11-21
JP2015519045A (ja) 2015-07-09
EP2841602A1 (en) 2015-03-04
WO2013159145A1 (en) 2013-10-31
US9411929B2 (en) 2016-08-09
EP2841602A4 (en) 2015-12-09
JP6262203B2 (ja) 2018-01-17

Similar Documents

Publication Publication Date Title
Tam et al. Robust global microRNA expression profiling using next-generation sequencing technologies
Uyar et al. Cumulus and granulosa cell markers of oocyte and embryo quality
Chatterjee et al. A cross comparison of technologies for the detection of microRNAs in clinical FFPE samples of hepatoblastoma patients
Jung et al. Robust microRNA stability in degraded RNA preparations from human tissue and cell samples
JP6666852B2 (ja) 前立腺がん再発の予後に関する遺伝子発現パネル
US9495515B1 (en) Algorithms for disease diagnostics
Kakimoto et al. MicroRNA stability in postmortem FFPE tissues: quantitative analysis using autoptic samples from acute myocardial infarction patients
JP2016178921A (ja) 遺伝子発現を用いた前立腺癌の予後を定量化する方法
CN104769130A (zh) 一种确定rna完整性的方法
CN108474033B (zh) 来源于体液的miRNA的品质的评价方法
CN108323184A (zh) 验证生物标志物测量
Roth Quantifying gene expression
AU2018344761A1 (en) A method of diagnosis, staging and monitoring of melanoma using microrna gene expression
CN109576350B (zh) 一种dna与rna同时定量的试剂盒、方法及质控方法
Jackson et al. qPCR-based mitochondrial DNA quantification: influence of template DNA fragmentation on accuracy
Talebi et al. Fusion transcript discovery using RNA sequencing in formalin-fixed paraffin-embedded specimen
JPWO2020027098A1 (ja) 体液検体の品質評価方法
Goulter et al. Evaluation of low density array technology for quantitative parallel measurement of multiple genes in human tissue
WO2015179777A2 (en) Gene expression profiles associated with sub-clinical kidney transplant rejection
US10253369B2 (en) Predicting likelihood of response to combination therapy
Vandesompele et al. Real-time PCR: current technology and applications
Vanhauwaert et al. RT-qPCR gene expression analysis in zebrafish: Preanalytical precautions and use of expressed repetitive elements for normalization
Pagliarulo et al. Sensitivity and reproducibility of standardized-competitive RT-PCR for transcript quantification and its comparison with real time RT-PCR
US20060024705A1 (en) Molecular analysis of hair follicles for disease
CN114990215A (zh) microRNA生物标志物在肺癌诊断或预测预后中的应用

Legal Events

Date Code Title Description
C06 Publication
PB01 Publication
EXSB Decision made by sipo to initiate substantive examination
SE01 Entry into force of request for substantive examination
WD01 Invention patent application deemed withdrawn after publication
WD01 Invention patent application deemed withdrawn after publication

Application publication date: 20150708