CN104764721A - Water body fluorescence material measurement apparatus - Google Patents
Water body fluorescence material measurement apparatus Download PDFInfo
- Publication number
- CN104764721A CN104764721A CN201410012214.0A CN201410012214A CN104764721A CN 104764721 A CN104764721 A CN 104764721A CN 201410012214 A CN201410012214 A CN 201410012214A CN 104764721 A CN104764721 A CN 104764721A
- Authority
- CN
- China
- Prior art keywords
- fluorescence
- water body
- water
- light
- interference filter
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Landscapes
- Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
Abstract
The invention relates to a water body fluorescence material measurement apparatus, which mainly comprises an LED lamp for exciting fluorescence, a long wave passing-through interference filter for separating the excitation light and the fluorescence, a photodiode for measuring the fluorescence intensity, and a control treatment unit. According to the present invention, the excitation light is subjected to reflection through the interference filter and then irradiates on a sample, and the fluorescence emitted by the sample absorbing the excitation light can penetrate through the interference filter and then is received by a detector. The whole set of the apparatus has characteristics of compact structure, high fluorescence receiving efficiency, and precise measurement, and is suitable for on-site detection.
Description
Technical field
This patent relate to a kind of device measured for water body fluorescent material.
Technical background
China mainland coastline length is 1.8 ten thousand kilometers, 1.4 ten thousand kilometers, shore line, island, there is the administered marine territory of 3,000,000 square kilometres, also has Changjiang River and Huanghe River two great river simultaneously, water resources total amount is at the forefront in the world, but the per-capita quantity of the resources is far below world average level, China is water resources shortage state in this.In order to Appropriate application water resources, need to detect water quality, as oxygen level, salinity, chlorophyll, pollutant etc.Usually there is a lot of methods to carry out water quality detection, have chemical method, physical method, biological method etc.Wherein spectroscopic applications is more extensive.Spectral measurement is simple and quick, non-contact measurement, and measurement result direct digitization stores, transmission.Spectrometer can be placed on aircraft or satellite, can carry out Quick Measurement, long term monitoring to water field of big area.
Spectrometer for environment measurement has a variety of, such as imaging spectrometer, fluorescence spectrophotometer etc.Here mainly fluorescence spectrophotometer is said.Different material has different level structures, and can transit on high level after by the optical excitation of applicable wavelength, general high level is unstable, toward ground state transition, can send fluorescence simultaneously.The level structure of different material is different, and the spectrum of the fluorescence sent is also different, so can determine composition and the content of material by measuring fluorescence spectrum.
Utilize fluorescence principle can measure certain content of material in water, normally do like this, this material of light source irradiation of this material fluorescence effectively can be excited with one, fluorescence is collected in vertical direction with a convergent lens, and filter exciting light with optical filter, go out the power of fluorescence with sensor measurement, then obtain the concentration of this material in water by computer digital animation.
Summary of the invention
This patent is used for the measurement of fluorescent material in water.Have in device one with the chamber of ft connection, water here face can circulate arbitrarily, can measure the situation of real-time water quality like this, and the light in the external world can not enter in chamber.Transparent panel 6 in the side in chamber, Main Function be that the optical system of water and device inside is separated, exciting light and fluorescence can pass through simultaneously.
The opposite side of dividing plate is optical system, has a LED 1 as excitation source, chooses the light of applicable wavelength as exciting light according to the needs of measured material.The light that LED sends is radiated at the 45 ° of long-pass of placing that tilt and crosses on interference filter 2, the cutoff wavelength of optical filter is greater than the wavelength of exciting light, so exciting light can be completely reflected on transparent panel 6, be irradiated to inside water and go, a convergent lens 3 is had between transparent panel 6 and optical filter 2, it converges to the light that LED sends inside water, water is under the irradiation of exciting light, impurity inside water is stimulated generation fluorescence, part fluorescence gets back to lens through transparent panel, be irradiated on optical filter 2 after convergence, the cutoff wavelength of long-pass filtration mating plate 2 is less than the wavelength of fluorescence, so fluorescence can be irradiated on photodiode 5 through filter plate completely, the photocurrent produced is input to data collecting card, computer disposal is inputted after digitizing.
The filter plate used in this device is interfere type filter plate, and it reflects completely to the light below cutoff wavelength, the light that cutoff wavelength is greater than to wavelength completely through, its transmission curve is as Fig. 2 and Fig. 3.Wherein Fig. 2 is that exciting light is perpendicular to transmission curve during optical filter incidence, the transmission curve of cutoff wavelength when about 600nm, Fig. 3 are exciting light oblique incidence, cutoff wavelength meeting blue shift, general is about 580nm, and the inclined degree of band edge is mild a little compared with during vertical incidence.As detected the chlorophyll a in water, LED sends the exciting light of 470nm, and owing to being in the cut-off region of optical filter, the incident light of 470nm will be reflected on sample.Launch the fluorescence of 680nm after the absorption of sample exciting light of 470nm, fluorescence is in the transmission bands of optical filter, and what almost can can't harm is detected device through optical filter and receives, so the fluorescence that photodiode 5 receives can be stronger, improves signal to noise ratio (S/N ratio).
Accompanying drawing explanation
Fig. 1 is the structural representation of this device, and wherein 1 is exciting light LED, and 2 cross interference filter for long-pass, and 3 is convergent lens, and 4 is water body to be measured, and 5 is photodiode, and 6 is transparent panel, and 7 is controlled processing unit.Fig. 2 is that exciting light is perpendicular to transmission curve during optical filter incidence, transmission curve when Fig. 3 is exciting light oblique incidence, blue shift can be there is compared with when can find out the cutoff wavelength of optical filter during oblique incidence and vertical incidence, but because the excitation wavelength in practical application and wavelength of fluorescence are all away from cutoff wavelength, so a little movement of cutoff wavelength can not exert an adverse impact to practical application.
Embodiment
Device schematic diagram as shown in Figure 1.In actual applications, with the chamber of external communication in water body inflow device to be measured, chamber is semi-hermetic, water can circulate, and consider the Chlorophyll-a Content in detection water, the exciting light of the 470nm that LED sends is reflected by optical filter, converge through lens, be irradiated on water body to be measured through transparent baffle, in water, test substance sends the fluorescence of 680nm after absorbing incident light, and fluorescence is detected device through transparent baffle and optical filter and receives.
In order to the light reducing environment enters reasons for its use noise after system, this device takes following methods: produce exciting light by periodic signal driving LED, detect the light having exciting light and receive without photodiode during exciting light, computing machine, by eliminating ground unrest after algorithm process, obtains the concentration of measurement of species.
The invention has the advantages that: use one piece of filter plate and lens to complete light path, utilize the character of optical filter exciting light and flashlight to be separated cleverly, the use of element reduces, thus makes apparatus structure compacter, stable; Interference filter in device to the light reflectivity of specific wavelength and transmitance very high, thus the fluorescence signal that photoelectricity part receives is stronger, makes to measure more accurate.
Claims (2)
1. a water body fluorescent material measurement mechanism, is characterized in that: the LED of a fluorescence excitation; A long-pass crosses interference filter; The lens of a converges activation light and fluorescence; The photodiode of a measurement fluorescence intensity.
2. a kind of water body fluorescent material measurement mechanism according to claim 1, is characterized in that: optical filter tilts 45 ° and places, and the light sent by LED reflexes on sample completely, and the luminous energy that sample sends is completely through this optical filter.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201410012214.0A CN104764721A (en) | 2014-01-07 | 2014-01-07 | Water body fluorescence material measurement apparatus |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201410012214.0A CN104764721A (en) | 2014-01-07 | 2014-01-07 | Water body fluorescence material measurement apparatus |
Publications (1)
Publication Number | Publication Date |
---|---|
CN104764721A true CN104764721A (en) | 2015-07-08 |
Family
ID=53646686
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CN201410012214.0A Pending CN104764721A (en) | 2014-01-07 | 2014-01-07 | Water body fluorescence material measurement apparatus |
Country Status (1)
Country | Link |
---|---|
CN (1) | CN104764721A (en) |
Citations (7)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN1727878A (en) * | 2004-07-28 | 2006-02-01 | 中国科学院大连化学物理研究所 | A kind of detector of fluorescence induced by light-emitting diode in high brightness |
CN1916604A (en) * | 2006-09-01 | 2007-02-21 | 中国科学院安徽光学精密机械研究所 | Method and device for classified detecting density of phytoplankton under water in site |
CN201535776U (en) * | 2009-02-09 | 2010-07-28 | 马义才 | Quantitative detection system based on test strip marked with constantly illuminating material |
CN102095712A (en) * | 2010-12-21 | 2011-06-15 | 无锡荣兴科技有限公司 | Photoelectric sensor for detecting blue-green algae |
CN102279174A (en) * | 2011-07-15 | 2011-12-14 | 中国科学院苏州纳米技术与纳米仿生研究所 | Alga identification and measurement sensor and method |
CN103091298A (en) * | 2013-02-01 | 2013-05-08 | 厦门大学 | Real-time fluorescent quantitative PCR detection system |
CN103175815A (en) * | 2013-03-06 | 2013-06-26 | 浙江大学 | Multi-wavelength LED-induced fluorescence tea quality nondestructive testing method and device |
-
2014
- 2014-01-07 CN CN201410012214.0A patent/CN104764721A/en active Pending
Patent Citations (7)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN1727878A (en) * | 2004-07-28 | 2006-02-01 | 中国科学院大连化学物理研究所 | A kind of detector of fluorescence induced by light-emitting diode in high brightness |
CN1916604A (en) * | 2006-09-01 | 2007-02-21 | 中国科学院安徽光学精密机械研究所 | Method and device for classified detecting density of phytoplankton under water in site |
CN201535776U (en) * | 2009-02-09 | 2010-07-28 | 马义才 | Quantitative detection system based on test strip marked with constantly illuminating material |
CN102095712A (en) * | 2010-12-21 | 2011-06-15 | 无锡荣兴科技有限公司 | Photoelectric sensor for detecting blue-green algae |
CN102279174A (en) * | 2011-07-15 | 2011-12-14 | 中国科学院苏州纳米技术与纳米仿生研究所 | Alga identification and measurement sensor and method |
CN103091298A (en) * | 2013-02-01 | 2013-05-08 | 厦门大学 | Real-time fluorescent quantitative PCR detection system |
CN103175815A (en) * | 2013-03-06 | 2013-06-26 | 浙江大学 | Multi-wavelength LED-induced fluorescence tea quality nondestructive testing method and device |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
CN101706434B (en) | Fluorophototmeter capable of detecting light absorption or light transmission | |
CN105021588B (en) | A kind of single light source CARS gas-detecting devices and method | |
CN101074921A (en) | Multifunctional light-absorbing, scattering and transmitting spectrograph based on surface plasma wave | |
CN106769882B (en) | Oil spilling monitor and monitoring method thereof | |
CN107462566B (en) | Raman spectrometer for detecting specific narrow wavenumber range | |
CN102798621A (en) | Multi-piece reflection type ultraviolet induced biological fluorescence detection system | |
CN103424387A (en) | Fluorescence spectrum detection device for pesticide residue | |
CN108627495B (en) | Fixed wavelength Raman scattering rapid acquisition and imaging device | |
CN106092895A (en) | A kind of water body chlorophyll concentration in situ detection device and detection method thereof | |
CN104597011B (en) | Excitation light source drift correction device and XRF | |
CN206074449U (en) | A kind of spot shaping optical system for fluorescence analyser | |
CN203443886U (en) | Three-color fluorescence detector based on white-light LED (Light-Emitting Diode) | |
CN105092836B (en) | Up-conversion luminescence immuno-chromatography detection device and detection method | |
CN108226113A (en) | Effective UV excites fluorescence measuring device | |
CN104792754A (en) | Detection device and method adopting lased-induced liquid fluorescence | |
CN110887814B (en) | Underwater turbidity detection method based on spectral analysis | |
CN106970058A (en) | The minimal feeding instrument and detection method in a kind of pair of fluorescent emission face | |
CN210571971U (en) | Portable laser Raman fluorometer system for monitoring water environment pollution and water environment pollution monitoring device | |
CN106525804A (en) | Optical structure of fluorescence immunochromatography instrument | |
CN204374087U (en) | A kind of Raman spectrum test macro based on liquid core waveguide | |
CN104764721A (en) | Water body fluorescence material measurement apparatus | |
US9121800B2 (en) | Internally reflective chamber for fluorescent radiation collection and concentration, and method for using the same | |
CN206684048U (en) | A kind of minimal feeding instrument in double fluorescent emission faces | |
CN108152252B (en) | Integral femtosecond time resolution fluorescence life measuring spectrometer | |
RU2010136979A (en) | SYSTEM AND METHOD OF DETECTION OF LIGHT |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
C06 | Publication | ||
PB01 | Publication | ||
EXSB | Decision made by sipo to initiate substantive examination | ||
SE01 | Entry into force of request for substantive examination | ||
WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20150708 |
|
WD01 | Invention patent application deemed withdrawn after publication |