CN104757118A - Peanut milk suitable for elderly to drink and preparation method thereof - Google Patents

Peanut milk suitable for elderly to drink and preparation method thereof Download PDF

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Publication number
CN104757118A
CN104757118A CN201510092598.6A CN201510092598A CN104757118A CN 104757118 A CN104757118 A CN 104757118A CN 201510092598 A CN201510092598 A CN 201510092598A CN 104757118 A CN104757118 A CN 104757118A
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China
Prior art keywords
peanut
milk
protein
prebiotics
preparation
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CN201510092598.6A
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Chinese (zh)
Inventor
战玉东
张继怀
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Shandong Gold Bean Or Pea (groundnut Products) Co Ltd
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Shandong Gold Bean Or Pea (groundnut Products) Co Ltd
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Priority to CN201510092598.6A priority Critical patent/CN104757118A/en
Publication of CN104757118A publication Critical patent/CN104757118A/en
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Abstract

The invention discloses a peanut milk suitable for the elderly to drink and a preparation method thereof. The peanut milk comprises limited enzymolysis phosphorylation modified peanut protein, limited enzymolysis milk and prebiotics, wherein the prebiotics consists of peanut non-starch polysaccharides, peanut flavonoids, peanut proanthocyanidins and peanut yellow pigment and tea polysaccharide. The preparation method of the peanut milk includes: conducting microwave-assisted compound protease limited enzymolysis on phosphorylation modified peanut protein and milk; adding the prebiotics into enzymolysis peanut protein and milk, performing high-pressure homogenization, and carrying out instantaneous ultra high temperature sterilization, cooling and aseptic canning. According to the invention, peanut protein and milk are subjected to limited enzymolysis, macromolecular protein turns into small molecular protein and protein peptide, the peanut milk has high protein content and is easy to digest, and is suitable for the characteristics of decreased digestive function of the elderly and high requirements for protein quality, quantity and nutritional value. The added prebiotics has the efficacy of resisting oxidation, clearing free radicals, lowering blood lipid and blood sugar, thus being able to prevent and treat senile diseases.

Description

The peanut milk that a kind of applicable the elderly drinks and preparation method
Technical field
The present invention relates to peanut milk and preparation method that a kind of applicable the elderly drinks, belong to peanut emulsion drink processing technique field.
Background technology
Peanut is one of main oil crops of China, is a kind of industrial crops of edible, oil expression dual-purpose, in world's agricultural production and trade, occupies critical role.Peanut protein is the vegetable protein that a kind of nutritive value is higher.About have 10% albumin in peanut protein, all the other 90% are basic protein.Grouts after peanut oil expression contain albumen 45-50%, adopt alkali lixiviate isoelectric precipitation technology can extract from peanut dregs purity of protein 90% peanut protein isolate.But the functional character of peanut protein isolate is poor, the needs of modern food industry development can not be met.Phosphorylation is the comparatively effective method of modifying of one, the phosphorylation of protein is the active group utilizing protein side chain selectively, introduce a large amount of phosphate groups, add the electronegativity of protein, improve the electrostatic repulsion between protein molecule, make it more easily to disperse in food system, mutual repulsion, thus improves solubility, coagulation stability, reduce isoelectric point, this Application and Development for peanut protein is very favourable.Therefore, the present invention utilizes phosphorylation modified peanut protein as peanut milk raw material, because the dissolubility, emulsibility etc. of phosphorylation modified peanut protein is all higher than peanut protein isolate, it well can dissolve each other with milk, simplify the processing process of peanut milk, the fat content of phosphorylation modified peanut protein is less than 1%, and duration of storage not easily produces lamination, improves the quality of peanut milk.
The elderly along with the age increasing, many elderly there will be some digestive problems, and gastrointestinal function goes down, and require higher to the quality and quantity of protein, and milk drink can provide protein compared with high-quality for the elderly.But common milk drink protein content is 2%, and milk protein molecule is comparatively large, if the elderly has lactose intolerance, just nondigestible.In addition, the high fat of blood of the elderly, hyperglycaemia, hypertensive " three high diseases " illness rate are higher, and except controlling with medicine, health care preventive and therapeutic action is at ordinary times also very important.Therefore, the present invention is based on and should provide high-quality, digestible milk protein beverage to the elderly, also to take into account prevention and therapy effect of the elderly's " three high diseases " simultaneously, adopt compound protease limited enzymatic hydrolysis phosphorylation modified peanut protein and milk protein, obtain protein content is high, protein molecular is little peanut, milk protein drink, in this protein drink, add prebiotics component again, play the effect of prevention and therapy " three high diseases ".
Summary of the invention
The object of the invention is to go down for solving the elderly's gastrointestinal function, lactose intolerance person increase, drink ordinary milk or vegetable protein milk beverage is nondigestible, simultaneously, the high fat of blood of the elderly, hyperglycaemia, hypertensive " three high diseases " patient lean on merely the difficult problems such as chemoprophylaxis and treatment easily lead to complications, the peanut milk providing a kind of applicable the elderly to drink and preparation method.Peanut milk of the present invention adopts limited enzymatic hydrolysis phosphorylation peanut protein and milk protein to be primary raw material, phosphorylation modified peanut protein and milk protein are after compound protease limited enzymatic hydrolysis, high molecular weight protein molecule becomes small molecular protein molecule or protein peptides, albumen not only solubility improves, and very easily digest and assimilate, further, protein peptides has anti-oxidant, scavenging free radicals, reducing blood lipid and hypoglycemic effect.The prebiotics added in peanut milk contains peanut SNSP, peanut flavonoids, peanut OPC, peanut uranidin and tea polysaccharide, these five kinds of materials are the native functional activity compositions extracted from peanut meal, peanut shell, peanut red coat, coarse tea leaf etc., and they have anti-oxidant, scavenging free radicals, reducing blood lipid and hypoglycemic functional activity.
For achieving the above object, the present invention by the following technical solutions:
The peanut milk that a kind of applicable the elderly drinks and preparation method, peanut milk component comprises phosphorylation modified peanut protein, milk and prebiotics, and wherein prebiotics is made up of peanut SNSP, peanut flavonoids, peanut OPC, peanut uranidin and tea polysaccharide.The preparation method of peanut milk comprises the following steps: (1) compound protease and phosphorylation modified peanut protein add in milk, under certain microwave power and temperature, microwave radiation technology enzymolysis peanut protein and milk certain hour, obtain limited enzymatic hydrolysis phosphorylation modified peanut protein and milk protein; (2) in the prebiotics peanut protein that adds enzymolysis and milk, high-pressure homogeneous, moment ultra high temperature sterilization, cooling, aseptic canning.
Preferably, the peanut milk that a kind of applicable the elderly drinks and preparation method, the protein content of described phosphorylation modified peanut protein is 90%-95%, and its volume ratio of adding quality and milk is 10:100-20:100(g/ml).
Preferably, the peanut milk that a kind of applicable the elderly drinks and preparation method, described prebiotics is by peanut SNSP, peanut flavonoids, peanut OPC, peanut uranidin and tea polysaccharide composition, the purity of its each component is all 80-90%, each weight percentages of components is: peanut SNSP 30%-40%, peanut flavonoids 15%-25%, peanut OPC 10%-20%, peanut uranidin 15%-25% and tea polysaccharide 20%-30%, prebiotics microcapsules are prepared into by atomizing freeze drying method, the volume ratio that prebiotics microcapsules add quality and milk is 0.1:100-0.5:100(g/ml).
Preferably, the peanut milk that a kind of applicable the elderly drinks and preparation method, compound protease described in step (1) is according to alkali protease (Alcalase, nominal vigor 2.4AU/g): flavor protease (Flavourzyme, nominal vigor 500LPAU/g): neutral proteinase (Neutral proteinase, nominal vigor 0.8AU/g) ratio of=2.5:3.2:2.7 is mixed to get compound protease, the addition of compound protease is 0.5-1.0ml/1000ml milk, natural ph, microwave radiation technology compound protease limited enzymatic hydrolysis technological parameter is microwave power 800-1000w, temperature 45-50 DEG C, time 3-5min.
Preferably, the peanut milk that a kind of applicable the elderly drinks and preparation method, the high-pressure homogeneous technological parameter described in step (2) is homogenizing temperature 45-50 DEG C, homogenization pressure 31-39MPa, and 135 DEG C of moment ultra high temperature sterilization 5s, are cooled to 40 DEG C, aseptic canning immediately.
The invention has the beneficial effects as follows: using the primary raw material of phosphorylation modified peanut protein as peanut milk, improve the protein content of peanut milk, for the elderly provides the peanut milk drink of daily desirable proteins quantity; By compound protease enzymolysis phosphorylation modified peanut protein and milk protein, make high molecular weight protein become small molecular protein or protein peptides, be easy to digest and assimilate, for the elderly provides the peanut milk drink of high protein quality; The native functional activity compositions such as peanut SNSP, peanut flavonoids, peanut OPC, peanut uranidin and tea polysaccharide are added in peanut milk, can prevention and therapy high fat of blood, hyperglycaemia, hypertension " three high diseases ", for the elderly provides the peanut milk be of high nutritive value drink.
Detailed description of the invention
embodiment 1
Get the phosphorylation modified peanut protein 117g that protein content is 92.7%, by according to alkali protease (Alcalase, nominal vigor 2.4AU/g): flavor protease (Flavourzyme, nominal vigor 500LPAU/g): neutral proteinase (Neutral proteinase, nominal vigor 0.8AU/g)=2.5:3.2:2.7 ratio composition compound protease 0.85ml, join in 1000ml milk, in microwave catalysis/synthesizer, microwave power 900w, enzymolysis 3.5min under temperature 47 DEG C of conditions, obtain limited enzymatic hydrolysis phosphorylation modified peanut protein and milk protein mixed liquor, according to weight percentage, getting peanut SNSP 30%(purity is 85.6%), peanut flavonoids 15%(purity is 82.4%), peanut OPC 10%(purity is 86.7%), peanut uranidin 15%(purity is 81.3%) and tea polysaccharide 30%(purity be 84.8%) mixing, prebiotics microcapsules are prepared into by atomizing freeze drying method, getting prebiotics microcapsules 0.25g joins in limited enzymatic hydrolysis phosphorylation modified peanut protein obtained above and milk protein mixed liquor, with temperature 47 DEG C in homogenizer, pressure 32MPa homogeneous, carry out 135 DEG C of moment ultra high temperature sterilization 5s afterwards, be cooled to 40 DEG C immediately, aseptic canning obtains the peanut milk that applicable the elderly drinks.The protein peptides percentage composition that this peanut milk middle-molecular-weihydroxyethyl is less than 10kDa is 23.4%; Antioxidation activity in vitro comprises: to the IC of DPPH free radical scavenging activity, Scavenging action to hydroxyl free radical, ultra-oxygen anion free radical clearance rate, iron ion chelation percent, chelating copper ions rate, lipid peroxidation inhibiting rate, Fe3+ reduction power and molybdenum reducing power 50value is respectively 8.56mg/L, 0.32mg/L, 7.15mg/L, 3.21mg/L, 1.48mg/L, 9.47mg/L, 6.62mg/L and 5.17mg/L; Function of blood sugar reduction activity comprises: the external IC to alpha-glucosaccharase enzyme inhibition activity 50value is 31.25mg/L, and after alloxan hyperglycemia model mouse peritoneal injection 200mg/kg body weight peanut milk 21d, blood sugar declines 87.5%, and hepatic glycogen rolls up.
embodiment 2
Get the phosphorylation modified peanut protein 200g that protein content is 93.6%, by according to alkali protease (Alcalase, nominal vigor 2.4AU/g): flavor protease (Flavourzyme, nominal vigor 500LPAU/g): neutral proteinase (Neutral proteinase, nominal vigor 0.8AU/g)=2.5:3.2:2.7 ratio composition compound protease 0.77ml, join in 1000ml milk, in microwave catalysis/synthesizer, microwave power 800w, enzymolysis 3min under temperature 45 C condition, obtain limited enzymatic hydrolysis phosphorylation modified peanut protein and milk protein mixed liquor, according to weight percentage, getting peanut SNSP 33%(purity is 90%), peanut flavonoids 15%(purity is 80%), peanut OPC 12%(purity is 90%), peanut uranidin 20%(purity is 90%) and tea polysaccharide 20%(purity be 90%) mixing, prebiotics microcapsules are prepared into by atomizing freeze drying method, getting prebiotics microcapsules 0. 5g joins in limited enzymatic hydrolysis phosphorylation modified peanut protein obtained above and milk protein mixed liquor, with temperature 45 C in homogenizer, pressure 34MPa homogeneous, carry out 135 DEG C of moment ultra high temperature sterilization 5s afterwards, be cooled to 40 DEG C immediately, aseptic canning obtains the peanut milk that applicable the elderly drinks.The protein peptides percentage composition that this peanut milk middle-molecular-weihydroxyethyl is less than 10kDa is 29.3%; Antioxidation activity in vitro comprises: to the IC of DPPH free radical scavenging activity, Scavenging action to hydroxyl free radical, ultra-oxygen anion free radical clearance rate, iron ion chelation percent, chelating copper ions rate, lipid peroxidation inhibiting rate, Fe3+ reduction power and molybdenum reducing power 50value is respectively 7.31mg/L, 0.85mg/L, 6.36mg/L, 4.55mg/L, 2.57mg/L, 6.31mg/L, 2.32mg/L and 6.89mg/L; Function of blood sugar reduction activity comprises: the external IC to alpha-glucosaccharase enzyme inhibition activity 50value is 25.15mg/L, and after alloxan hyperglycemia model mouse peritoneal injection 200mg/kg body weight peanut milk 21d, blood sugar declines 88.9%, and hepatic glycogen rolls up.
embodiment 3
Get the phosphorylation modified peanut protein 158g that protein content is 95%, by according to alkali protease (Alcalase, nominal vigor 2.4AU/g): flavor protease (Flavourzyme, nominal vigor 500LPAU/g): neutral proteinase (Neutral proteinase, nominal vigor 0.8AU/g)=2.5:3.2:2.7 ratio composition compound protease 1.0ml, join in 1000ml milk, in microwave catalysis/synthesizer, microwave power 1000w, enzymolysis 5min under temperature 50 C condition, obtain limited enzymatic hydrolysis phosphorylation modified peanut protein and milk protein mixed liquor, according to weight percentage, getting peanut SNSP 40%(purity is 80%), peanut flavonoids 15%(purity is 90%), peanut OPC 10%(purity is 81.5%), peanut uranidin 15%(purity is 86.8%) and tea polysaccharide 20%(purity be 87.8%) mixing, prebiotics microcapsules are prepared into by atomizing freeze drying method, getting prebiotics microcapsules 0.1g joins in limited enzymatic hydrolysis phosphorylation modified peanut protein obtained above and milk protein mixed liquor, with temperature 50 C in homogenizer, pressure 39MPa homogeneous, carry out 135 DEG C of moment ultra high temperature sterilization 5s afterwards, be cooled to 40 DEG C immediately, aseptic canning obtains the peanut milk that applicable the elderly drinks.The protein peptides percentage composition that this peanut milk middle-molecular-weihydroxyethyl is less than 10kDa is 25.4%; Antioxidation activity in vitro comprises: to the IC of DPPH free radical scavenging activity, Scavenging action to hydroxyl free radical, ultra-oxygen anion free radical clearance rate, iron ion chelation percent, chelating copper ions rate, lipid peroxidation inhibiting rate, Fe3+ reduction power and molybdenum reducing power 50value is respectively 6.77mg/L, 0.12mg/L, 4.21mg/L, 3.79mg/L, 1.86mg/L, 4.25mg/L, 4.46mg/L and 4.18mg/L; Function of blood sugar reduction activity comprises: the external IC to alpha-glucosaccharase enzyme inhibition activity 50value is 33.09mg/L, and after alloxan hyperglycemia model mouse peritoneal injection 200mg/kg body weight peanut milk 21d, blood sugar declines 90.3%, and hepatic glycogen rolls up.
embodiment 4
Get the phosphorylation modified peanut protein 100g that protein content is 90%, by according to alkali protease (Alcalase, nominal vigor 2.4AU/g): flavor protease (Flavourzyme, nominal vigor 500LPAU/g): neutral proteinase (Neutral proteinase, nominal vigor 0.8AU/g)=2.5:3.2:2.7 ratio composition compound protease 0. 5ml, join in 1000ml milk, in microwave catalysis/synthesizer, microwave power 1000w, enzymolysis 4min under temperature 49 DEG C of conditions, obtain limited enzymatic hydrolysis phosphorylation modified peanut protein and milk protein mixed liquor, according to weight percentage, getting peanut SNSP 30%(purity is 82.5%), peanut flavonoids 15%(purity is 86.3%), peanut OPC 10%(purity is 80%), peanut uranidin 15%(purity is 80%) and tea polysaccharide 30%(purity be 80%) mixing, prebiotics microcapsules are prepared into by atomizing freeze drying method, getting prebiotics microcapsules 0.38g joins in limited enzymatic hydrolysis phosphorylation modified peanut protein obtained above and milk protein mixed liquor, with temperature 49 DEG C in homogenizer, pressure 31MPa homogeneous, carry out 135 DEG C of moment ultra high temperature sterilization 5s afterwards, be cooled to 40 DEG C immediately, aseptic canning obtains the peanut milk that applicable the elderly drinks.The protein peptides percentage composition that this peanut milk middle-molecular-weihydroxyethyl is less than 10kDa is 27.6%; Antioxidation activity in vitro comprises: to the IC of DPPH free radical scavenging activity, Scavenging action to hydroxyl free radical, ultra-oxygen anion free radical clearance rate, iron ion chelation percent, chelating copper ions rate, lipid peroxidation inhibiting rate, Fe3+ reduction power and molybdenum reducing power 50value is respectively 9.03mg/L, 0.69mg/L, 8.33mg/L, 2.19mg/L, 2.01mg/L, 8.16mg/L, 7.11mg/L and 3.57mg/L; Function of blood sugar reduction activity comprises: the external IC to alpha-glucosaccharase enzyme inhibition activity 50value is 24.51mg/L, and after alloxan hyperglycemia model mouse peritoneal injection 200mg/kg body weight peanut milk 21d, blood sugar declines 89.5%, and hepatic glycogen rolls up.
The above, be only the specific embodiment of the present invention, is not limited thereto, and is anyly familiar with those skilled in the art in the technical scope that the present invention discloses, and can expect change easily or replace, all should be encompassed within protection scope of the present invention.

Claims (5)

1. the peanut milk drunk of an applicable the elderly and preparation method; peanut milk component comprises phosphorylation modified peanut protein, milk and prebiotics, and wherein prebiotics is made up of peanut SNSP, peanut flavonoids, peanut OPC, peanut uranidin and tea polysaccharide; The preparation method of peanut milk comprises the following steps: (1) adopts microwave radiation technology compound protease limited enzymatic hydrolysis phosphorylation modified peanut protein and milk; (2) prebiotics adds in enzymolysis peanut protein and milk, high-pressure homogeneous, instantaneously ultra high temperature sterilization, cooling, aseptic canning.
2. the peanut milk drunk of a kind of applicable the elderly according to claim 1 and preparation method, it is characterized in that, the protein content of phosphorylation modified peanut protein is 90%-95%, and its volume ratio of adding quality and milk is 10:100-20:100(g/ml).
3. the peanut milk drunk of a kind of applicable the elderly according to claim 1 and preparation method, it is characterized in that, described prebiotics is by peanut SNSP, peanut flavonoids, peanut OPC, peanut uranidin and tea polysaccharide composition, the purity of its each component is all 80-90%, each weight percentages of components is: peanut SNSP 30%-40%, peanut flavonoids 15%-25%, peanut OPC 10%-20%, peanut uranidin 15%-25% and tea polysaccharide 20%-30%, prebiotics microcapsules are prepared into by atomizing freeze drying method, the volume ratio that prebiotics microcapsules add quality and milk is 0.1:100-0.5:100(g/ml).
4. the peanut milk drunk of a kind of applicable the elderly according to claim 1 and preparation method, it is characterized in that, compound protease described in step (1) is according to alkali protease (Alcalase, nominal vigor 2.4AU/g): flavor protease (Flavourzyme, nominal vigor 500LPAU/g): neutral proteinase (Neutral proteinase, nominal vigor 0.8AU/g) ratio of=2.5:3.2:2.7 is mixed to get compound protease, the addition of compound protease is 0.5-1.0ml/1000ml milk, natural ph, microwave radiation technology compound protease limited enzymatic hydrolysis technological parameter is microwave power 800-1000w, temperature 45-50 DEG C, time 3-5min.
5. the peanut milk drunk of a kind of applicable the elderly according to claim 1 and preparation method, it is characterized in that, high-pressure homogeneous technological parameter described in step (2) is homogenizing temperature 45-50 DEG C, homogenization pressure 31-39MPa, 135 DEG C of moment ultra high temperature sterilization 5s, be cooled to 40 DEG C immediately, aseptic canning.
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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9615596B2 (en) 2016-03-14 2017-04-11 Kraft Foods Group Brands Llc Protein products and methods for making the same

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CN1058883A (en) * 1991-09-02 1992-02-26 吉林省农业科学院大豆研究所 The production technology of peanut-made cooling drink
CN1076345A (en) * 1992-03-17 1993-09-22 烟台市栖霞植物油脂总厂 The manufacture method of animal/vegetable protein solid beverage
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CN102217700A (en) * 2011-05-27 2011-10-19 山东省花生研究所 Method for preparing peanut protein
CN102550676A (en) * 2010-12-16 2012-07-11 苏州工业园区尚融科技有限公司 Production method of low-sugar probiotic peanut milk
CN103988904A (en) * 2014-06-16 2014-08-20 山东禹王生态食业有限公司 Inactive double-protein lactobacillus beverage and preparation method thereof
CN104068124A (en) * 2013-03-26 2014-10-01 沭阳县苏合农产品销售专业合作联社 Preparation method for peanut milk beverage

Patent Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1058883A (en) * 1991-09-02 1992-02-26 吉林省农业科学院大豆研究所 The production technology of peanut-made cooling drink
CN1076345A (en) * 1992-03-17 1993-09-22 烟台市栖霞植物油脂总厂 The manufacture method of animal/vegetable protein solid beverage
CN1526290A (en) * 2003-03-06 2004-09-08 湖南亚华种业股份有限公司 High protein peptide milk powder and its production process
CN102550676A (en) * 2010-12-16 2012-07-11 苏州工业园区尚融科技有限公司 Production method of low-sugar probiotic peanut milk
CN102217700A (en) * 2011-05-27 2011-10-19 山东省花生研究所 Method for preparing peanut protein
CN104068124A (en) * 2013-03-26 2014-10-01 沭阳县苏合农产品销售专业合作联社 Preparation method for peanut milk beverage
CN103988904A (en) * 2014-06-16 2014-08-20 山东禹王生态食业有限公司 Inactive double-protein lactobacillus beverage and preparation method thereof

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9615596B2 (en) 2016-03-14 2017-04-11 Kraft Foods Group Brands Llc Protein products and methods for making the same
US11533927B2 (en) 2016-03-14 2022-12-27 Kraft Foods Group Brands Llc Protein products and methods for making the same

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Application publication date: 20150708