CN104743761B - Method for alleviating density of float sludge of silt port by using heterotrophic bacteria microorganisms - Google Patents
Method for alleviating density of float sludge of silt port by using heterotrophic bacteria microorganisms Download PDFInfo
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- CN104743761B CN104743761B CN201510107964.0A CN201510107964A CN104743761B CN 104743761 B CN104743761 B CN 104743761B CN 201510107964 A CN201510107964 A CN 201510107964A CN 104743761 B CN104743761 B CN 104743761B
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- mud scum
- muddy bottoms
- closely knit
- heterotroph
- extracellular polymeric
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F11/00—Treatment of sludge; Devices therefor
- C02F11/02—Biological treatment
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2103/00—Nature of the water, waste water, sewage or sludge to be treated
- C02F2103/007—Contaminated open waterways, rivers, lakes or ponds
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- Hydrology & Water Resources (AREA)
- Engineering & Computer Science (AREA)
- Environmental & Geological Engineering (AREA)
- Water Supply & Treatment (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Purification Treatments By Anaerobic Or Anaerobic And Aerobic Bacteria Or Animals (AREA)
Abstract
The invention discloses a method for alleviating density of float sludge of a silt port by using heterotrophic bacteria microorganisms. The method comprises the following steps: I) screening heterotrophic bacteria which can generate extracellular polymeric substances and alleviate float sludge of the silt port from float sludge of the silt port; and II) generating the extracellular polymeric substances by heterotrophic bacteria and carrying out interaction on extracellular polymeric substances and the float sludge of the silt port so as to alleviate the dense speed of the float sludge of the silt port. By selecting the heterotrophic bacteria microorganisms of the float sludge of the silt port, interaction on extracellular polymeric substances (EPS) and the float sludge of the silt port is carried out to alleviate the dense speed of the float sludge of the silt port, so that the application period of navigational depth is prolonged, the maintaining and dredging period can be prolonged, the operational cost of port enterprises is lowered, and the economical benefit is improved.
Description
Technical field
The present invention relates to a kind of slow down the closely knit method of Muddy Bottoms harbour mud scum, it is more particularly to a kind of to utilize the micro- life of heterotroph
Thing slows down the closely knit method of Muddy Bottoms harbour mud scum.
Background technology
For Muddy Bottoms harbour, the alluvial of silt can be navigated by water to yard craft and be brought very big impact, and periodic cleaning harbour
Alluvial not only wastes a large amount of human and material resources, fund, has an effect on environment.And mud scum has certain mobility, can be little by severe
Use as the depth of water in the mud scum of seaworthiness severe and do not affect ship operation performance, so as to extend Interval of Maintenance Dredging, be harbour
Enterprise saves substantial amounts of maintenance dredging expense.But mud scum can be As time goes on and constantly closely knit, causes the severe value of mud scum
Gradually increase, can not use as the depth of water when more than seaworthiness severe value, be the application cycle for extending seaworthy water depth, improve suitable
The value of the boat depth of water, it is considered to delay the closely knit of mud scum using microorganism.There have been Muddy Bottoms harbour in foreign countries using inorganic
Chemoautotrophic bacteria is delaying the closely knit speed of mud scum, and then increases the application time of seaworthy water depth, generates huge economic effect
Benefit, but the growth of inorganic chemoautotrophic bacteria need to depend on the growth of its own, growth is extremely slow, yields poorly, results in the need for
Can just tell on up to the time of several years.Therefore seed selection more suitably microorganism is needed, particularly grows heterotroph faster,
The closely knit of mud scum is slowed down by the extracellular polymeric (EPS) of heterotroph generation, is extended seaworthy water depth and is utilized the cycle, reduce harbour
The number of times of desilting, saves fund, improves the economic benefit of port enterprise.
It is domestic that microorganism is used in into the research in terms of waste water control, by retrieval, find it is related to the present invention as
Under several open source literatures, but they be all using microorganism accelerate water in material sinking speed, this with the present invention delay float
The closely knit difference for having essence of mud.
One) on June 2nd, 1993, Application No.：92112087.7 patent application document disclose a kind of sediment settlement
Agent and production method, this application are related to a kind of sewage-treating agent containing silt and production method.Its principal character：In terms of composition it
Except with addition to polyacrylamide, initiator and deionized water, also using amphoteric starch, NaOH and phosphoric acid.In process aspect,
Synthesize polyacrylamide with redox system initiator, amphoteric starch is with polyacrylamide by hydrolyzing grafting, then phosphorylation.
The invention advantage is：In the case of same sedimentation, the present invention is only the half of existing polyacrylamide sedimentation agent consumption, and is settled
Speed wants fast 0.25-1 times.The invention is the sinking speed for accelerating the pollutant such as silt in sewage using microorganism, with the present invention
The closely knit speed of mud scum that slows down have essential distinction.
Two) on October 13rd, 2002, the patent document CN1170483C of Patent No. CN98802182.X discloses one
The method for improving sinkability of fish food pellets is planted, a kind of method of the settleability for improving fish food particle is the invention provides, including
Sugar aqueous solution is coated on fish food particle surface.Liquid glucose can with the concentration of 1-10%, vacuum or normal pressure under, plus be with or without day
So coat in the case of grease.The most handy liquid glucose natural containing sucrose and beet molasses is used as preferred sugar aqueous solution.This
Bright is to accelerate the sinking speed that water fish eats particle using microorganism, has essential area with the closely knit speed of mud scum that slows down of the present invention
Not.
Three) on April 4th, 2012, Application No.：201110350564.4 patent application document disclose a kind of improvement
Composite bacteria agent of activated sludge settling property and preparation method thereof, the disclosure of the invention is a kind of to improve activated sludge settling property
Composite bacteria agent, this flocculation microbial inoculum flocculation ability are strong, nuisanceless.Good collaboration effect can be produced with the original microorganism of activated sludge
Should, improve the settling property of activated sludge.The invention be using microorganism shorten activated sludge hydraulic detention time, Ye Jijia
Fast sinking speed, has essential distinction with the closely knit speed of mud scum that slows down of the present invention.
The content of the invention
The present invention provides a kind of simply and easily micro- using heterotroph for solving technical problem present in known technology
Biology slows down the closely knit method of Muddy Bottoms harbour mud scum.
The present invention is adopted the technical scheme that by solving technical problem present in known technology：One kind utilizes heterotroph
Microorganism slows down the closely knit method of Muddy Bottoms harbour mud scum, comprises the following steps：
One) from the mud scum at Muddy Bottoms harbour, screening can extracellular polymer and can be to the closely knit generation of Muddy Bottoms harbour mud scum
Slow down the heterotroph of effect；
Two) heterotrophism bacteria microorganism is made to produce extracellular polymeric and make extracellular polymeric with Muddy Bottoms harbour mud scum phase interaction
With to slow down the closely knit speed of Muddy Bottoms harbour mud scum.
The step 2), heterotroph is cultivated in the lab：Heterotrophism mother bacterial liquid is prepared first, makes heterotroph female at which
Extracellular polymeric is produced in liquid, obtains the zymotic fluid rich in extracellular polymeric, then zymotic fluid is mixed with Muddy Bottoms harbour mud scum
Close, make extracellular polymeric interact with Muddy Bottoms harbour mud scum, to slow down the closely knit speed of Muddy Bottoms harbour mud scum.
The step 2), heterotroph microbial inoculant to be cultivated in the mud scum of Muddy Bottoms harbour, heterotroph is in development growth
During constantly secret out of extracellular polymeric, extracellular polymeric is interacted with Muddy Bottoms harbour mud scum, floats Muddy Bottoms harbour
Mud slows down closely knit speed.
The heterotroph is Burkholderia.
The present invention has the advantages and positive effects that：From the micro- life of heterotroph for coming from Muddy Bottoms harbour mud scum itself
Thing so as to which the extracellular polymeric (EPS) of generation is interacted with Muddy Bottoms harbour mud scum, slows down the close of Muddy Bottoms harbour mud scum
Real speed, so as to extend the application cycle of seaworthy water depth, can extend Interval of Maintenance Dredging, save the operation of port enterprise into
This, increases economic efficiency.And the material that the present invention is selected is microorganism and its product of naturally occurring in environment, will not be to ring
Border has a negative impact, simple to operation；Extracellular gathering can be produced by providing suitable growing environment control Institute of Micro-biology
The amount of compound, subjective controllability is strong, instant effect.
Description of the drawings
Fig. 1 is Muddy Bottoms harbour original state mud scum sample rate and the Muddy Bottoms harbour mud scum density after the application present invention with close
Changing trend diagram between in real time.
Specific embodiment
For the content of the invention, feature and effect of the present invention can be further appreciated that, following examples are hereby enumerated, and coordinates accompanying drawing
Describe in detail as follows：
Embodiment 1：
A kind of utilization heterotrophism bacteria microorganism slows down the closely knit method of Muddy Bottoms harbour mud scum, comprises the following steps：
One) from the mud scum at Muddy Bottoms harbour, screening can extracellular polymer and can be to the closely knit generation of Muddy Bottoms harbour mud scum
Slow down the heterotroph of effect；
The present embodiment, can extracellular polymer and can be to mud scum with bacterium colony with the mud scum fetched from Muddy Bottoms harbour as material
It is closely knit produce to slow down act as index and screen from mud scum sample obtaining Burkholderia.
Two) heterotroph is made to produce extracellular polymeric and make extracellular polymeric interact with Muddy Bottoms harbour mud scum, to subtract
The closely knit speed of slow Muddy Bottoms harbour mud scum.
In the present embodiment, above-mentioned steps specific practice two) is：Heterotroph is cultivated in the lab：Prepare first
Heterotrophism mother bacterial liquid, makes heterotroph produce extracellular polymeric in its mother liquor, obtains the Burkholderia rich in extracellular polymeric and ferments
Then Burkholderia zymotic fluid is mixed by liquid with Muddy Bottoms harbour mud scum, makes extracellular polymeric mutual with Muddy Bottoms harbour mud scum
Effect, to slow down the closely knit speed of Muddy Bottoms harbour mud scum.Specifically Examination on experimental operation is：Will be enriched in the primary of extracellular polymeric
Kirschner fermented liquid is hybridly prepared into density for 1050kg/m with Muddy Bottoms harbour mud scum3Aggregate sample, measure EPS in aggregate sample
Content is 5.64g/L, and aggregate sample is put into a diameter of 0.15m, highly closely knit for standing in the closely knit pipe of 1m, record muddy water boundary
Face changes over trend, and draws mud scum density and change over relation curve, verifies the effect for delaying mud scum closely knit, as a result
Such as Fig. 1.With Muddy Bottoms harbour seaworthiness severe value as 12250N/m3As a example by, density value is converted into for 1250kg/m3, the present embodiment float
Mud is still not up to 1250kg/m through 270 hours (11 days) closely knit its density3, and using the original without Burkholderia zymotic fluid
Shape mud scum sample controls group is closely knit to seaworthiness severe to only need about 14 hours, it can be seen that within the whole sedimentation time, microorganism
Secreted extracellular polymeric substantially slow down the closely knit speed of mud scum, so as to extend the application cycle of seaworthy water depth.
Embodiment 2：
A kind of utilization heterotrophism bacteria microorganism slows down the closely knit method of Muddy Bottoms harbour mud scum, comprises the following steps：
One) from the mud scum at Muddy Bottoms harbour, screening can extracellular polymer and can be to the closely knit generation of Muddy Bottoms harbour mud scum
Slow down the heterotroph of effect；
The present embodiment, can extracellular polymer and can be to mud scum with bacterium colony with the mud scum fetched from Muddy Bottoms harbour as material
It is closely knit produce to slow down act as index and screen from mud scum sample obtaining Burkholderia.
Two) heterotroph is made to produce extracellular polymeric and make extracellular polymeric interact with Muddy Bottoms harbour mud scum, to subtract
The closely knit speed of slow Muddy Bottoms harbour mud scum.
In the present embodiment, above-mentioned steps specific practice two) is：Heterotroph is inoculated in the mud scum of Muddy Bottoms harbour and is trained
Support, heterotroph constantly secrets out of extracellular polymeric during development growth, and extracellular polymeric is mutual with Muddy Bottoms harbour mud scum
Effect, makes Muddy Bottoms harbour mud scum slow down closely knit speed.Specifically Examination on experimental operation is：Burkholderia mother liquor is prepared first,
Then Burkholderia mother liquor is added to into density for 1050kg/m3Mud scum in, and periodically add necessary nutrient solution and make micro- life
Thing development growth, Burkholderia fall and constantly secret out of extracellular polymeric in growth course, reach when extracellular polymeric concentration is measured
During to 2.34g/L, take mud scum sample and be put into a diameter of 0.15m, highly closely knit for standing in the closely knit pipe of 1m, record muddy water boundary
Face changes over trend, and draws silt density and change over relation curve, verifies the effect for delaying mud scum closely knit, as a result
Such as Fig. 1.With Muddy Bottoms harbour seaworthiness severe value as 12250N/m3As a example by, density value is converted into for 1250kg/m3, the present embodiment float
Mud it is closely knit to density be 1250kg/m341 hours (1.7 days) are taken around, and control group is only needed about 14 hours, it can be seen that
In the whole closely knit time, the extracellular polymeric of Institute of Micro-biology's secretion substantially slow down the closely knit speed of mud scum, suitable so as to extend
The application cycle of the boat depth of water.
Although being described to the preferred embodiments of the present invention above in conjunction with accompanying drawing, the invention is not limited in
The specific embodiment stated, above-mentioned specific embodiment are only schematic, be not it is restricted, this area it is common
Technical staff in the case of without departing from present inventive concept and scope of the claimed protection, may be used also under the enlightenment of the present invention
To make many forms, these are belonged within protection scope of the present invention.
Claims (3)
1. a kind of utilization heterotrophism bacteria microorganism slows down the closely knit method of Muddy Bottoms harbour mud scum, it is characterised in that including following step
Suddenly：
One) from the mud scum at Muddy Bottoms harbour screening can extracellular polymer and closely knit to Muddy Bottoms harbour mud scum producing slow down
The heterotroph of effect, the heterotroph are Burkholderia；
Two) heterotroph is made to produce extracellular polymeric and make extracellular polymeric interact with Muddy Bottoms harbour mud scum, to slow down silt
The closely knit speed of shale harbour mud scum.
2. utilization heterotrophism bacteria microorganism according to claim 1 slows down the closely knit method of Muddy Bottoms harbour mud scum, its feature
It is, the step 2), heterotroph is cultivated in the lab：Heterotrophism mother bacterial liquid is prepared first, makes heterotroph in its mother liquor
Extracellular polymeric is produced, the zymotic fluid rich in extracellular polymeric is obtained, then zymotic fluid is mixed with Muddy Bottoms harbour mud scum, is made
Extracellular polymeric is interacted with Muddy Bottoms harbour mud scum, to slow down the closely knit speed of Muddy Bottoms harbour mud scum.
3. utilization heterotrophism bacteria microorganism according to claim 1 slows down the closely knit method of Muddy Bottoms harbour mud scum, its feature
Be, the step 2), by heterotroph be inoculated in the mud scum of Muddy Bottoms harbour cultivate, heterotroph during development growth not
Disconnected to secret out of extracellular polymeric, extracellular polymeric is interacted with Muddy Bottoms harbour mud scum, makes Muddy Bottoms harbour mud scum slow down close
Real speed.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201510107964.0A CN104743761B (en) | 2015-03-12 | 2015-03-12 | Method for alleviating density of float sludge of silt port by using heterotrophic bacteria microorganisms |
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| CN201510107964.0A CN104743761B (en) | 2015-03-12 | 2015-03-12 | Method for alleviating density of float sludge of silt port by using heterotrophic bacteria microorganisms |
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| CN104743761B true CN104743761B (en) | 2017-03-22 |
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Families Citing this family (4)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN105668807A (en) * | 2016-03-01 | 2016-06-15 | 云南圣清环保科技有限公司 | Method adopting microorganisms for treating coal chemical industrial wastewater |
| CN106746415A (en) * | 2017-01-10 | 2017-05-31 | 天津科技大学 | A kind of simple and easy method for delaying Muddy Bottoms harbour mud scum closely knit |
| CN106746425A (en) * | 2017-01-12 | 2017-05-31 | 交通运输部天津水运工程科学研究所 | A kind of method that utilization methylotrophic bacteria extends seaworthy water depth use time |
| CN109207395B (en) * | 2018-09-11 | 2021-02-02 | 天津科技大学 | Methylophagus strain and application thereof |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE102005045407A1 (en) * | 2005-09-23 | 2007-03-29 | Wurpts, Rewert, Dipl.-Ing. | In situ conditioning by remedial dredging method for maintenance of navigable depths in air-locks, outer ports, sea lanes and rivers, comprises ventilation of silt/mud present at bed of the water bodies by contacting with atmospheric oxygen |
| CN102250776A (en) * | 2011-06-14 | 2011-11-23 | 南京农业大学 | Acid-resistance heterotrophic bacterium strain Z3 used for bioleaching treatment of sludge and livestock and poultry manure |
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2015
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Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE102005045407A1 (en) * | 2005-09-23 | 2007-03-29 | Wurpts, Rewert, Dipl.-Ing. | In situ conditioning by remedial dredging method for maintenance of navigable depths in air-locks, outer ports, sea lanes and rivers, comprises ventilation of silt/mud present at bed of the water bodies by contacting with atmospheric oxygen |
| CN102250776A (en) * | 2011-06-14 | 2011-11-23 | 南京农业大学 | Acid-resistance heterotrophic bacterium strain Z3 used for bioleaching treatment of sludge and livestock and poultry manure |
Non-Patent Citations (1)
| Title |
|---|
| 异养菌与自养菌对好氧颗粒污泥稳定性的影响;李志华等;《土木建筑与环境工程》;20101031;第32卷(第5期);第77页左栏第1段，第79页左栏第1段 * |
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