CN104805155B - With laminari-oligo saccharide and its preparation method and the application for promoting sea cucumber growth and degeneration-resistant effect - Google Patents
With laminari-oligo saccharide and its preparation method and the application for promoting sea cucumber growth and degeneration-resistant effect Download PDFInfo
- Publication number
- CN104805155B CN104805155B CN201510259443.7A CN201510259443A CN104805155B CN 104805155 B CN104805155 B CN 104805155B CN 201510259443 A CN201510259443 A CN 201510259443A CN 104805155 B CN104805155 B CN 104805155B
- Authority
- CN
- China
- Prior art keywords
- laminari
- sea cucumber
- oligo saccharide
- preparation
- sea
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Landscapes
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
- Fodder In General (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
The invention provides a kind of laminari-oligo saccharide and its preparation method and application for having and promoting sea cucumber growth and degeneration-resistant effect, it is related to microorganism field.It is 2846 through pretreatment of raw material, the laminari-oligo saccharide mean molecule quantity that prepared by crude enzyme liquid, laminari-oligo saccharide preparation process is obtained；Average degree of polymerization is 7；Feed addictive is used as by the use of the laminari-oligo saccharide, sea cucumber can be promoted to grow and improve sea cucumber anti-adversity ability, increase its resistance and immunity function, remarkably promote the growth rate of sea cucumber, make sea cucumber increased weight 48 76%, and take and add the sea cucumber growth conditions of the oligosaccharides bait preferably, pierce strong, body is mellow and full, and the laminari-oligo saccharide is preferable sea cucumber bait additive.
Description
Technical field
The present invention relates to microorganism field, and in particular to a kind of to have the laminari-oligo saccharide for promoting sea cucumber growth and degeneration-resistant effect
And its preparation method and application.
Background technology
Sea-tangle is a kind of kelp, contains three kinds of main laminarins, respectively seaweed gel, sea-tangle carbohydrate gum and sea-tangle
Starch, early in 1881, Englishman isolated seaweed gel from sea-tangle；Kylin isolates sea-tangle carbohydrate gum within 1913, at about
Phase isolates laminarin.Laminari-oligo saccharide is, by laminarin is degraded, to be degraded to obtain oligosaccharides with polysaccharide, not only dissolubility
It is good, and show more preferable physiologically active.
Sea cucumber contains multiple nutritional components, and its acid mucopolysaccharide, arginine and chondroitin content are abundant, delaying sanility,
Anticancer and raising body immunity.Growth with the whole world to the demand of sea cucumber, causes sea cucumber overfishing so that sea cucumber
Aquaculture is developed rapidly, and the rise of aquaculture causes the demand increase to feed, has been reported display, microalgae is fed to sea cucumber
Or tangleweed, it can accelerate the growth of sea cucumber, add the seawood meal of different proportion, can also make uptake rate of the sea cucumber to feed, life
Long performance and apparent digestibility etc. play certain active promoting function.During propagating artificially, the disease of sea cucumber is also therewith
Produce, holothruian cultures bottleneck is occurred, bacterium, fungi and parasite moment threaten the health of sea cucumber.Therefore need badly on the market
A kind of preferable sea cucumber bait additive for the degeneration-resistant effect that sea cucumber can be promoted to grow and sea cucumber can be improved.
The content of the invention
To solve the above problems, first purpose of the present invention, which is to provide one kind, has the growth of promotion sea cucumber and degeneration-resistant effect
Laminari-oligo saccharide preparation method and itself.It is a further object to provide added using above-mentioned laminari-oligo saccharide in feed
Plus the application in agent.
Technical scheme is as follows：It is a kind of that there is the growth of promotion sea cucumber and the preparation side of the laminari-oligo saccharide of degeneration-resistant effect
Method, is realized by following steps：
(1) pretreatment of raw material：Sea-tangle surface silt is rinsed well, is cut into small pieces, is immersed in water；
(2) prepared by crude enzyme liquid：By laminari-oligo saccharide degradation bacteria (Achromobacter sp：H0-5) with 1% (v/v) inoculation
Amount is inoculated on fermentation medium, fermentation condition：20-25 DEG C of temperature, dissolved oxygen amount is 60-80%, speed of agitator 150r/min；
Fermentation medium is (w/v) in described step (2)：Sodium alginate 0.5%, peptone 0.3%, sodium chloride
0.5%, MgSO4·7H2O 0.4%, KH2PO40.8%, CaCl20.2%, pH are 7.2.
Described laminari-oligo saccharide degradation bacteria (Achromobacter sp：H0-5) preserving number is：CGMCC No:10179.
Described laminari-oligo saccharide degradation bacteria strains H0-5 is isolated from Daliang City of LiaoNing, China province marine site black stone reef surrounding waters
Surface seawater and the seaweed sample that swims, are identified as achromobacter (Achromobacter), are named as Achromobacter
sp:H0-5, described laminari-oligo saccharide degradation bacteria submits preservation on December 15th, 2014, and specific preservation information is as follows：
Depositary institution's title：China Committee for Culture Collection of Microorganisms's common micro-organisms center (CGMCC)；
Depositary institution address：Yard 1, BeiChen xi Road, Chaoyang District, Beijing City 3, Institute of Microorganism, Academia Sinica；
Preservation date：On December 15th, 2014；
Deposit number：CGMCC No.10179.
Described laminari-oligo saccharide degradation bacteria H0-5 physiology characteristics are：Gram-Negative bacillus, single bacterium colony is rounded, surface
Smooth, water profit, neat in edge, in milk yellow, haloing is produced on surrounding plate.
(3) prepared by laminari-oligo saccharide：Sea-tangle after being handled in step (1) is soluble in water, add thick enzyme by 4% enzyme amount that connects
Liquid, adds the sodium chloride of water volume 0.5%, is degraded in being shaken on 20-25 DEG C of shaking table, rotating speed 150r/min, and degradation time is
24-48h, after enzyme digestion reaction terminates, suction filtration, spray drying obtain alga oligosaccharides powder.
Laminari-oligo saccharide average relative molecular mass prepared by the present invention is 2846；Average degree of polymerization is 7.
The application in feed addictive using laminari-oligo saccharide is also claimed in the present invention：
(1) sea cucumber is fed using obtained laminari-oligo saccharide as additive, it is possible to increase the degeneration-resistant effect of sea cucumber.
(2) sea cucumber is fed using obtained laminari-oligo saccharide as additive, it is possible to increase the growth rate of sea cucumber.
The beneficial effects of the invention are as follows：The method of the present invention has high efficiency, and product is easy to control, facility is simple, power consumption is low
The advantages of, the laminari-oligo saccharide obtained by the inventive method is used as feed addictive, and sea cucumber can be promoted to grow and improve sea cucumber
Anti-adversity ability, increases its resistance and immunity function, remarkably promotes the growth rate of sea cucumber, makes sea cucumber increased weight 48-76%, and
And take and add the sea cucumber growth conditions of the oligosaccharides bait preferably, pierce strong, body is mellow and full, present invention gained laminari-oligo saccharide is preferable
Sea cucumber bait additive.
Brief description of the drawings
The laminari-oligo saccharide thin layer chromatography analysis result that Fig. 1 is prepared for the present invention；
Fig. 2 improves the influence of the degeneration-resistant effect of sea cucumber for laminari-oligo saccharide prepared by the present invention；
Influences of the Fig. 3 for the laminari-oligo saccharide of the invention prepared to sea cucumber body weight.
Embodiment
Technical scheme is further described with specific embodiment below, but the present invention is not in any form
It is limited to embodiment content.Experimental method described in embodiment is conventional method unless otherwise specified；Unless otherwise specified,
The reagent and biomaterial, are commercially obtained.
Laminari-oligo saccharide degradation bacteria (the Achromobacter sp of embodiment 1：H0-5 seed selection)
1st, bacteria selection：
The enrichment of appropriate marine alga, rotten sea-tangle and silt is taken from Chinese Daliang City of Liaoning Province marine site black stone reef surrounding waters
Nutrient solution 1mL, gradient dilution is carried out with sterile physiological saline, then by 10-2~10-6Dilution respectively take 0.2mL to be applied to
It is incubated in 30 DEG C on Selective agar medium, there are depression or the single bacterium colony of haloing to carry out condition optimizing around picking, screening is obtained
Obtain the bacterial strain for the sea-tangle that can degrade.Selective agar medium formula (w/v) is as follows：Sodium alginate 2%, CaCl20.1%th, NaCl
1.5%th, KH2PO40.05%th, MgSO4·7H2O 0.05%, FeSO4·7H2O 0.001%, agar 1%, pH is 7.2.
The bacterial strain is gram-Negative bacillus, and single bacterium colony is rounded, and surface is smooth, water profit, neat in edge, in milk yellow,
Haloing is produced on surrounding plate.The measurement result of bacterial strain physiological and biochemical index is as shown in table 1.According to the data of table 1 and 16S
RDNA sequence datas, achromobacter (Achromobacter) is accredited as by the bacterial strain.
The bacterial strain HO-5 Physiology and biochemistry qualification results of table 1
2. the preparation of laminari-oligo saccharide
(1) pretreatment of raw material
Sea-tangle surface silt is rinsed well, 1 cubic centimetre of fritter is cut into, is immersed in water；
(2) prepared by crude enzyme liquid
The strain H0-5 of low salt concn after isolating and purifying is inoculated with liquid fermentation medium into 25 DEG C to cultivate 24 hours,
Dissolved oxygen amount is 70%, speed of agitator 150r/min, is passed on 1% inoculum concentration, continuous passage 7 times, treats that bacterial strain state is steady
After fixed, zymotic fluid is centrifuged at 4 DEG C, rotating speed is 5000r/min, centrifuge 30min, gained supernatant is crude enzyme liquid.Fermentation
Liquid is crude enzyme liquid, and the enzyme activity that bacterial strain is produced reaches 51.498U/mL.
Liquid fermentation medium used is (w/v)：Sodium alginate 0.5%, peptone 0.3%, NaCl 0.5%,
MgSO4·7H2O 0.4%, KH2PO40.8%, CaCl20.2%, pH are 7.2.
(3) prepared by laminari-oligo saccharide
Sea-tangle 40g after being handled in step (1) is dissolved in 300ml running water, crude enzyme liquid is added by 4% enzyme amount that connects,
The sodium chloride of water volume 0.5% is added, in 20 DEG C of shaking tables, 150r/min rotation concussion 24-48h, with degradation time
The trend for first raising and reducing afterwards is presented in extension, laminari-oligo saccharide, and total reducing sugar is substantially the same with reduced sugar variation tendency, and in 36h, sugar contains
Measure highest.After enzyme digestion reaction terminates, suction filtration separates sea-tangle and oligosaccharides, spray drying obtains laminari-oligo saccharide powder；
It is as shown in Figure 1 through TLC identification result：Left-hand point 1 is dextrose standard sample, and right-hand point 2 is laminari-oligo saccharide,
Oligosaccharide molecular amount distribution situation can be drawn from Fig. 1, and visible four of laminari-oligo saccharide of degrading clearly oligosaccharides congruent point, molecular weight is big
It is small, it is separated clearly, no conditions of streaking；Total reducing sugar and content of reducing sugar show that oligosaccharides average degree of polymerization is 7, average mark after measured
Son amount is 2846.
The sea cucumber administering transgenic of embodiment 2
1. by laminari-oligo saccharide as additive, coordinate in basal feed.Five groups of experiment point, oligosaccharides addition is respectively 0,
0.5%, 2.5%, 5%, 10%.Sea cucumber body weight is fed once between 2.60 ± 0.70g, daily, and feeding amount is sea cucumber body weight
3-5%.Water is changed to sea cucumber once, cleaning residual bait and excrement daily.Real-time monitored water temperature and room temperature, observe sea cucumber state, take the photograph
Food and defecation situation.
2. laminari-oligo saccharide it is degeneration-resistant to sea cucumber and growth influence
When testing 10d, because sea cucumber is like a fish out of water and the change of feeding manner, cause sea cucumber Body weight loss, oligosaccharides
Shown in group and the degeneration-resistant growing state result figure 2 of control group sea cucumber, degeneration-resistant growth rate refers to when sea cucumber grows 10 days and original body weight
The ratio of difference.It was found that laminari-oligo saccharide can improve the degeneration-resistant effect of sea cucumber, than not adding the sea cucumber of laminari-oligo saccharide in adverse circumstances
Lower growth 20-25%, after testing 8 weeks, weighs each group sea cucumber body weight, draws the average value of each group sea cucumber body weight, it is found that sea-tangle is few
Sugar can be obviously promoted the growth of sea cucumber, and growth rate reaches 48% to 76%, and growth rate increases and raised with oligosaccharides addition,
And the growth rate of control group sea cucumber is only 7.3%, as a result such as Fig. 3.
In summary, growth of the laminari-oligo saccharide to sea cucumber, which serves, remarkably promotes：Sea-tangle is prepared by enzymic degradation sea-tangle
Oligosaccharides；Basal feed is coordinated to feed sea cucumber with different oligosaccharides additions, oligosaccharides addition group sea cucumber average weight is dramatically increased,
Growth rate raises resistance with increasing for oligosaccharides addition preferably, and activities of antioxidant enzymes is improved, and correlation factor activity is immunized and rises
It is high.
Annex：
Sequence table
SEQ ID No.1 (laminari-oligo saccharide degradation bacteria strains H0-5 16S rRNA nucleotide sequences)
ATGCTTACACATGCAAGTCGAACGGCAGCACGGACTTCGGTCTGGTGGCGAGTGGCGAACGGGTGAGTAATGTATCG
GAACGTGCCTAGTAGCGGGGGATAACTACGCGAAAGCGTAGCTAAATACCGCATACCCCCTACCCCGGAAAGCAGGG
GATCGCAAGACCTTGCACTATTAGAGCGGCCGATATCGGATTAGCTAGTTGGTGGGGTAANGGCTCACCAAGGCGAC
GATCCGTAGCTGGTTTGAGAGGACGACCAGCCACACTGGGACTGAGACACGGCCCAGACTCCTACCCCAGGCAGCAG
TGGGGATATTTGGACAATGGGGGAACCTGATCCAGCCATCCCGCGTGTGCGATGAAGGCCTTCGGGTTGTAAAGCAC
TAATGGCAGGAAAGAAACGTCATGGGCTAATACCCCGTGAAACTGACGGTACCTGCAGAATAAGCACCGGCTAACTA
CGTGCCAGCAGCCGCGGTAATACGTAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGTGCGCAGGCGGTT
CGGAAAGAAAGATGTGAAATCCCAGAGCTTAACTTTGGAACTGCATTTTTAACTACCGAGCTAGAGTGTGTCAGAGG
GAGGTGGAATTCCGCGTGTAGCAGTGAAATGCGTAGATATGCGGAGGAACACCGATGGCGAAGGCAGCCTCCTGGGA
TAACACTGANNNTCATGCACGAAAGCGTGGGGAGCAAACAGGATTAGATACCCTGGTAGTCCACGCCCTAAACGATG
TCAACAAGCTGTTGGGGCCTTCGGGGTTGGTAGCGCANTAACGCGTGAAGTTGACCGCCTGGGGAGTAC
CCTCGCAAGATTAAAACTCAAAGGAATTGACGGGGACCCGCACAAGCGGTGGATGATGTGGATTAATTCGATGCAAC
GCGAAAAACCTTACCTTGGCTTGACATGTCTGGAATTCCGAAGAGATTTGGAAGTGCTCGCAAGAGAACCGGAACAC
AGGTGCTGCATGGCTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCTTGTCATT
AGTTGCTACGAAAGGGCACTCTAATGAGACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGACGTCAAGTCCTCAT
GGCCCTTATGGGTAGGGCTTCACACGTCATACAATGGTCGGGACAGAGGGTCGCCAACCCGCGAGGGGGAGCCAATC
CCAGAAACCCGATCGTAGTCCGGATCGCAGTCTGCAACTCGACTGCGTGAAGTCGGAATCGCTAGTAATCGCGGATC
AGCATGTCGCGGTGAATACGTTCCCGGGTCTTGTACACACCGCCCGTCACACCATG
Claims (5)
1. a kind of have the growth of promotion sea cucumber and the preparation method of the laminari-oligo saccharide of degeneration-resistant effect, it is characterised in that by following
Step is realized：
(1) pretreatment of raw material：Sea-tangle surface silt is rinsed well, is cut into small pieces, is immersed in water；
(2) prepared by crude enzyme liquid：It is CGMCC No by preserving number:10179 laminari-oligo saccharide degradation bacteria Achromobacter sp：
H0-5 is inoculated on fermentation medium with 1% (v/v) inoculum concentration, fermentation condition：20-25 DEG C of temperature, dissolved oxygen amount is 60-
80%, speed of agitator 150r/min；
(3) prepared by laminari-oligo saccharide：Sea-tangle after being handled in step (1) is soluble in water, crude enzyme liquid is added by 4% enzyme amount that connects,
The sodium chloride of water volume 0.5% is added, in vibrating degraded on 20-25 DEG C of shaking table, rotating speed 150r/min, degradation time is 24-
48h, after enzyme digestion reaction terminates, suction filtration, spray drying obtain alga oligosaccharides powder.
2. the preparation method of laminari-oligo saccharide according to claim 1, it is characterised in that fermentation training in described step (2)
It is (w/v) to support base：Sodium alginate 0.5%, peptone 0.3%, sodium chloride 0.5%, MgSO4·7H2O 0.4%, KH2PO4
0.8%, CaCl20.2%, pH are 7.2.
3. a kind of laminari-oligo saccharide that preparation method as claimed in claim 1 is obtained, it is characterised in that the laminari-oligo saccharide average mark
Son amount is 2846；Average degree of polymerization is 7.
4. a kind of application of laminari-oligo saccharide obtained using preparation method as claimed in claim 1 in feed addictive, its
It is characterised by, feeds sea cucumber using obtained laminari-oligo saccharide as additive, it is possible to increase the degeneration-resistant effect of sea cucumber.
5. a kind of application of laminari-oligo saccharide obtained using preparation method as claimed in claim 1 in feed addictive, its
It is characterised by, feeds sea cucumber using obtained laminari-oligo saccharide as additive, it is possible to increase the growth rate of sea cucumber.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510259443.7A CN104805155B (en) | 2015-05-20 | 2015-05-20 | With laminari-oligo saccharide and its preparation method and the application for promoting sea cucumber growth and degeneration-resistant effect |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510259443.7A CN104805155B (en) | 2015-05-20 | 2015-05-20 | With laminari-oligo saccharide and its preparation method and the application for promoting sea cucumber growth and degeneration-resistant effect |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN104805155A CN104805155A (en) | 2015-07-29 |
| CN104805155B true CN104805155B (en) | 2017-10-03 |
Family
ID=53690352
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201510259443.7A Active CN104805155B (en) | 2015-05-20 | 2015-05-20 | With laminari-oligo saccharide and its preparation method and the application for promoting sea cucumber growth and degeneration-resistant effect |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN104805155B (en) |
Families Citing this family (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106417924B (en) * | 2016-09-05 | 2019-08-16 | 河北省海洋与水产科学研究院 | Add the preparation method of the holothurian feed of scallop cylinder mould attachment |
| CN107736622A (en) * | 2017-10-09 | 2018-02-27 | 浙江海洋大学 | Laminari-oligo saccharide and its preparation method and application |
| CN111543549A (en) * | 2020-06-02 | 2020-08-18 | 烟台绿叶动物保健品有限公司 | Preparation method and application of traditional Chinese medicine microecological preparation |
| CN111876453A (en) * | 2020-08-10 | 2020-11-03 | 威海康博尔生物药业有限公司 | Preparation method of seaweed oligosaccharide |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103540540A (en) * | 2013-03-01 | 2014-01-29 | 浙江大学 | Kelp degrading bacteria and method for preparing kelp juice utilizing same |
| CN103834610A (en) * | 2014-01-28 | 2014-06-04 | 大连大学 | Preparation method of unicellular sea tangle powder and sea tangle oligosaccharides |
| CN103881952A (en) * | 2014-04-03 | 2014-06-25 | 大连理工大学 | Bacillus amyloliquefaciens and an application thereof as well as method for preparing kelp feed |
-
2015
- 2015-05-20 CN CN201510259443.7A patent/CN104805155B/en active Active
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103540540A (en) * | 2013-03-01 | 2014-01-29 | 浙江大学 | Kelp degrading bacteria and method for preparing kelp juice utilizing same |
| CN103834610A (en) * | 2014-01-28 | 2014-06-04 | 大连大学 | Preparation method of unicellular sea tangle powder and sea tangle oligosaccharides |
| CN103881952A (en) * | 2014-04-03 | 2014-06-25 | 大连理工大学 | Bacillus amyloliquefaciens and an application thereof as well as method for preparing kelp feed |
Non-Patent Citations (2)
| Title |
|---|
| Isolation of a novel alginate-degrading bacterium, Ochrobactrum sp. and production of alginate-degrading enzyme;Zhou Mao-hong et al;《Journal of life sciences》;20071231;第1卷(第1期);第14-17页 * |
| 海藻酸钠寡糖生物活性的研究进展;张玉娟等;《中国酿造》;20141231;第33卷(第1期);第5-8页 * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN104805155A (en) | 2015-07-29 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN101611767B (en) | Method for producing microbial fermentation bait for sea cucumbers | |
| CN101691550B (en) | Microbial inoculum for improving water body and structure of biological intestinal colony, and preparation method and application thereof | |
| CN105018544B (en) | With the carrageenan oligosaccharide and its preparation method and application for promoting sea cucumber growth and degeneration-resistant effect | |
| CN104805155B (en) | With laminari-oligo saccharide and its preparation method and the application for promoting sea cucumber growth and degeneration-resistant effect | |
| CN108865953A (en) | One plant of wide spectrum inhibits bacillus and its composite bacteria preparation of aquatic products vibrio pathogen | |
| CN102127514B (en) | Strong-stability moderate-temperature neutral alpha-amylase high-producing bacterium and zymologic property thereof | |
| CN107828698B (en) | Compound bacterium preparation and preparation method and application thereof | |
| CN106318880A (en) | Bacillus amyloliquefaciens and its bacterial depressant and use | |
| CN104911169B (en) | A kind of method for training cordyceps mycelium and fibrinolysin processed | |
| CN106434373A (en) | High-density fermentation medium formula of sparassis crispa and pharmaceutical grade glucan preparation method of high-density fermentation medium formula | |
| CN110106090B (en) | Neurospora crassa strain and application thereof | |
| CN108929859A (en) | One type bacterial strain of bacillus HB172198 and its application | |
| CN102845225A (en) | Hypsizygus marmoreus liquid strain fermenting technique | |
| CN101020895B (en) | Plant lactobacillus fermenting process for preparing ensilage with rich conjugated linoleic acid | |
| CN109456914A (en) | One kind low temperature resistant lactic acid bacteria used for aquiculture and its application | |
| CN106701645A (en) | Bacillus amyloliquefaciens B7 with immune growth promoting effect and application method of bacillus amyloliquefaciens B7 | |
| CN110305819A (en) | One plant of feather efficient degrading bacterial strain and its application | |
| CN104710206A (en) | Preparation method for volvariella volvacea liquid strain | |
| CN110093387A (en) | A method of chitosan oligosaccharide is prepared using chitin in bacillus and Paecilomyces varioti Synergistic degradation shrimp and crab shells | |
| CN106434475A (en) | Streptomyces polysaccharide degradation bacterium as well as culture method and application thereof | |
| CN1460423A (en) | Production method of straw and stalk microbial fermented feed | |
| CN109679873A (en) | A kind of serratia marcescens metabolite and the application as aquatic immune reinforcing agent | |
| CN1045043C (en) | Auricularia auricula strains and cultivation method thereof | |
| Uchida et al. | Distribution and characterization of bacteria capable of decomposing brown algae fronds in waters associated with Laminaria vegetation | |
| CN101838612B (en) | New geotrichum and application thereof in degradation of single-cell seaweed polysaccharide |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| EXSB | Decision made by sipo to initiate substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |