CN106417924B - Add the preparation method of the holothurian feed of scallop cylinder mould attachment - Google Patents
Add the preparation method of the holothurian feed of scallop cylinder mould attachment Download PDFInfo
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- CN106417924B CN106417924B CN201610802001.7A CN201610802001A CN106417924B CN 106417924 B CN106417924 B CN 106417924B CN 201610802001 A CN201610802001 A CN 201610802001A CN 106417924 B CN106417924 B CN 106417924B
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- cylinder mould
- mould attachment
- scallop cylinder
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- 241000237509 Patinopecten sp. Species 0.000 title claims abstract description 111
- 235000020637 scallop Nutrition 0.000 title claims abstract description 111
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- 239000000843 powder Substances 0.000 claims description 96
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- 238000003756 stirring Methods 0.000 claims description 23
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- 239000008103 glucose Substances 0.000 claims description 11
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- 108010080698 Peptones Proteins 0.000 claims description 8
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims description 8
- 230000003750 conditioning effect Effects 0.000 claims description 8
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- ZPWVASYFFYYZEW-UHFFFAOYSA-L dipotassium hydrogen phosphate Chemical compound [K+].[K+].OP([O-])([O-])=O ZPWVASYFFYYZEW-UHFFFAOYSA-L 0.000 claims description 5
- 229910052943 magnesium sulfate Inorganic materials 0.000 claims description 5
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- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 claims description 4
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- 235000013312 flour Nutrition 0.000 claims description 4
- 239000004615 ingredient Substances 0.000 claims description 4
- 150000003272 mannan oligosaccharides Chemical class 0.000 claims description 4
- 229910000402 monopotassium phosphate Inorganic materials 0.000 claims description 4
- 235000019796 monopotassium phosphate Nutrition 0.000 claims description 4
- PJNZPQUBCPKICU-UHFFFAOYSA-N phosphoric acid;potassium Chemical compound [K].OP(O)(O)=O PJNZPQUBCPKICU-UHFFFAOYSA-N 0.000 claims description 4
- 239000011780 sodium chloride Substances 0.000 claims description 4
- 238000009940 knitting Methods 0.000 claims description 3
- 210000002268 wool Anatomy 0.000 claims description 3
- 241000193830 Bacillus <bacterium> Species 0.000 claims description 2
- 241000726221 Gemma Species 0.000 claims description 2
- 239000000654 additive Substances 0.000 claims description 2
- 239000000203 mixture Substances 0.000 claims description 2
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 claims 1
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- 244000061456 Solanum tuberosum Species 0.000 description 5
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- 230000000694 effects Effects 0.000 description 4
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- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 description 4
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- 241000965254 Apostichopus japonicus Species 0.000 description 1
- 241001474374 Blennius Species 0.000 description 1
- 240000005717 Dioscorea alata Species 0.000 description 1
- VMHLLURERBWHNL-UHFFFAOYSA-M Sodium acetate Chemical compound [Na+].CC([O-])=O VMHLLURERBWHNL-UHFFFAOYSA-M 0.000 description 1
- 241001314440 Triphora trianthophoros Species 0.000 description 1
- 229930013930 alkaloid Natural products 0.000 description 1
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- 229910052500 inorganic mineral Inorganic materials 0.000 description 1
- CJWQYWQDLBZGPD-UHFFFAOYSA-N isoflavone Natural products C1=C(OC)C(OC)=CC(OC)=C1C1=COC2=C(C=CC(C)(C)O3)C3=C(OC)C=C2C1=O CJWQYWQDLBZGPD-UHFFFAOYSA-N 0.000 description 1
- 150000002515 isoflavone derivatives Chemical class 0.000 description 1
- 235000008696 isoflavones Nutrition 0.000 description 1
- 229940099596 manganese sulfate Drugs 0.000 description 1
- 239000011702 manganese sulphate Substances 0.000 description 1
- 235000007079 manganese sulphate Nutrition 0.000 description 1
- SQQMAOCOWKFBNP-UHFFFAOYSA-L manganese(II) sulfate Chemical compound [Mn+2].[O-]S([O-])(=O)=O SQQMAOCOWKFBNP-UHFFFAOYSA-L 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 235000013372 meat Nutrition 0.000 description 1
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- 229920001542 oligosaccharide Polymers 0.000 description 1
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- 238000006213 oxygenation reaction Methods 0.000 description 1
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- 239000001632 sodium acetate Substances 0.000 description 1
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- 235000014347 soups Nutrition 0.000 description 1
- 239000004575 stone Substances 0.000 description 1
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- YWYZEGXAUVWDED-UHFFFAOYSA-N triammonium citrate Chemical compound [NH4+].[NH4+].[NH4+].[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O YWYZEGXAUVWDED-UHFFFAOYSA-N 0.000 description 1
- 239000001393 triammonium citrate Substances 0.000 description 1
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Classifications
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
- Y02A40/818—Alternative feeds for fish, e.g. in aquacultures
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
- Y02P60/87—Re-use of by-products of food processing for fodder production
Landscapes
- Fodder In General (AREA)
Abstract
The present invention provides a kind of preparation methods of holothurian feed for adding scallop cylinder mould attachment, including pretreatment of raw material, biological quenched, curing five steps of quenched, second batch and granulating and forming, finally obtain the feed granules of finished product.Scallop cylinder mould attachment is added in holothurian feed by the preparation method of the holothurian feed of addition scallop cylinder mould attachment provided by the invention, it takes full advantage of the organic substances such as a variety of algae and shell clast in scallop cylinder mould attachment and provides nutrition for sea cucumber, not only the problems such as having realized waste utilization, saved resource, but also can effectively having solved occupied space caused by the random discarding of scallop cylinder mould attachment, pollution environment.Macromolecular substances in feed are degraded to small-molecule substance by the quenched fermentation by microorganism of biology, make feed be easier to be digested absorption, utilization rate is higher, and mitigates the stomach burden of sea cucumber；It cures the quenched water stability for making feed to greatly promote, to improve efficiency of feed utilization.
Description
Technical field
The invention belongs to holothurian feed field, in particular to a kind of preparation for the holothurian feed for adding scallop cylinder mould attachment
Method.
Background technique
With the fast development of holothruian cultures industry, holothurian feed develops from single algae powder to full man-made feeds, and sea cucumber is matched
The quality of bait material grows closely related with sea cucumber.China possesses scallop culture industry most huge in the world, and annual output is more than
10000000 tons.Behind of this number is a large amount of cultivation rubbish, that is, scallop cage attachment, if be not used with
Meaning abandons, and has not only polluted environment but also waste of resource.Research finds algae rich in scallop cylinder mould attachment, shell clast etc.
Nutriment, and scallop cylinder mould attachment is easy to get, is cheap, if it is possible to use it as holothurian feed, it will be one
Determine to reduce aquaculture cost in degree, while scallop cylinder mould attachment can also be solved and arbitrarily stack bring a series of problems.
Untreated natural feed is unfavorable for digesting and assimilating, directly since complicated component, macromolecular substances content are more
Feeding may result in sea cucumber and disease of digestive system occurs, seriously affect the weight gain and quality of sea cucumber.Authorization Notice No.
The patent of invention of CN103039727B discloses a kind of stichopus japonicus fermented feed of alternative seaweed, using Trichoderma, rhizopus, wine
Five kinds of brewer yeast, bacillus subtilis and lactic acid bacteria conventional bacterial classifications are used in combination, and carry out fermentation process to holothurian feed, make to raise
Material is easier to digest and assimilate.Bafillus natto and Leuconostoc mesenteroides are all to be widely used and apply in feed in food in recent years
Few microorganism fungus kind may generate unexpected if carrying out fermentation process to holothurian feed with these new-type strains
Effect.In addition, since sea cucumber is that the bait ingested in water, and once launched can not all be eaten up sometimes, if feed dissipates
Mistake rate is higher, and just more because losing caused by dissolution in water, the utilization rate for not only resulting in feed is low, can also cause water quality deterioration,
Influence the health status of sea cucumber.The rate of scattering and disappearing of feed in water, i.e. feed water stability, it is quenched by curing to a certain extent
It influences, the factors such as temperature, time of modulation play an important role to the stability of feed.Therefore, optimum feed tune is explored
Matter condition becomes those skilled in the art's urgent problem.
Summary of the invention
In order to solve the above-mentioned technical problems, the present invention provides a kind of systems of holothurian feed for adding scallop cylinder mould attachment
Preparation Method.
Specific technical solution of the present invention is as follows:
One aspect of the present invention provides a kind of preparation method of holothurian feed for adding scallop cylinder mould attachment, including as follows
Step:
(1) moss pre-processes: moss being cleaned with clear water, is placed in baking oven and is dried to water content less than 12%, with crushing
Machine crushes, and crosses 60 meshes, obtains moss powder；
(2) Zostera marina pre-processes: Zostera marina being cleaned with clear water, is placed in baking oven and is dried to water content less than 12%, use
Pulverizer crushes, and crosses 60 meshes, obtains Zostera marina powder；
(3) scallop cylinder mould attachment pre-processes: scallop cylinder mould attachment being cleaned with clear water, is placed in baking oven and dries, is used
Pulverizer crushes, and obtains scallop cylinder mould attachment powder；
The scallop cylinder mould attachment includes algae and shell clast, the parts by weight of the algae and the shell clast
Than for 1~2:1, the algae includes at least one of Enteromorpha, sea lettuce, helictite algae, boat-shaped algae, plumage algae or knitting wool algae；
(4) prepared by basal feed: by Zostera marina powder 20 made from 10~20 parts of moss powder made from step (1), step (2)
~40 parts, 45~65 parts of scallop cylinder mould attachment mixing made from step (3), stir evenly, and basal feed is made；
(5) biology is quenched: being inoculated with the microorganism fungus kind of 1~3% mass in Xiang Suoshu basal feed, is trained at 30 DEG C
It supports, obtains fermented feed；
(6) granulating and forming: the fermented feed is placed in 50 DEG C of drying in oven to moisture less than 12%, is crushed with feed
Machine carries out break process, obtains the finished product holothurian feed that partial size is 0.5mm.
Further, the preparation process of the basal feed further includes the kudzu-vine root powder and 3 added parts by weight and be 7~15 parts
~8 parts of okra powder.
Kudzu-vine root powder and okra powder can supplement sea cucumber isoflavones needed for growth and development, alkaloid and multivitamin
And minerals, sea cucumber growth can be promoted, improve trepang vigor.
Further, the quenched method of biology is as follows in the step (5): by the microorganism through overactivation and expansion culture
Strain is inoculated into the basal feed by 2% mass ratio, and sterile purified water is added in 50% ratio, and stirring is equal
It is even, it is placed in 24~48h of ferment at constant temperature in 30 DEG C of baking oven, obtains fermented feed.
Macromolecular substances in feed are degraded to easy to digest and absorb small point by the quenched fermentation by microorganism of biology
Sub- substance makes feed be easier to be digested absorption, and utilization rate is higher, and mitigates the stomach burden of sea cucumber
Further, the microorganism fungus kind is rhizopus, Trichoderma, saccharomyces cerevisiae, bacillus subtilis, natto gemma
The mixture of bacillus and Leuconostoc mesenteroides.
Further, it is 1:1:1:1:1:1 that the microorganism fungus kind, which is weight fraction ratio,.
Further, the quenched method of biology is as follows in the step (5): by the microorganism through overactivation and expansion culture
Strain is inoculated into the basal feed by 2% mass ratio, and sterile purified water is added in 50% ratio, and stirring is equal
It is even, culture medium is added, 24~48h of ferment at constant temperature in 30 DEG C of baking oven is placed in, obtains fermented feed；The culture medium includes such as
The ingredient of lower parts by weight: 10 parts of glucose, 10 parts of beef extract, 6 parts of peptone, 10 parts of malt extract, 10 parts of corn flour, chlorination
20 parts of sodium, 0.2 part of magnesium sulfate, 0.5 part of potassium dihydrogen phosphate, 0.3 part of dipotassium hydrogen phosphate.
It further, further include curing quenched step between the step (5) and the step (6), the curing is quenched
Method particularly includes: using efficient quality controling machine the fermented feed cure it is quenched, refining temperature be 95~105 DEG C, adjust
The matter time is 35~60s, obtains cured feed.
Cure it is quenched feed softening, starch gelatinization can be made by humid heat treatment, be conducive to feed and shape, and improve the water of feed
Middle stability, when application, will not dissolve at once, improve efficiency of feed utilization, reduce waste.
It further, further include addition additives step, the addition side of the auxiliary agent between the step (5) and step (6)
Method is as follows: adding bone tankage into the auxiliary agent by 4~8 times of quality of the auxiliary agent, adds and account for the bone tankage matter
The maize germ oil of amount 1.5%, is added to after mixing in the cured feed.
Auxiliary agent is added into feed using bone tankage as carrier, can be increased the particle of auxiliary agent, be improved auxiliary agent stability,
Disperse auxiliary agent more evenly in feed
Further, the auxiliary agent includes the ingredient of following parts by weight: 2.5 parts of mannan-oligosaccharides, galactooligosacchariwith 2.5
Part, dense plain 1 part and 0.5 part of saccharicter-penin of mandarin orange.
Oligosaccharide is added into feed, and the immunity and resistance of sea cucumber can be improved, improve the quality of sea cucumber.
Another aspect of the present invention provides the holothurian feed that scallop cylinder mould attachment is added according to made from the above method.
The beneficial effects of the present invention are: the preparation side of the holothurian feed of addition scallop cylinder mould attachment provided by the invention
Method takes full advantage of the organic substances such as a variety of algae and shell clast in scallop cylinder mould attachment and provides nutrition for sea cucumber,
On the one hand it realizes waste utilization, saved resource, another convenience can also effectively solve the random discarding of scallop cylinder mould attachment
Caused by occupied space, pollution environment the problems such as, while cost is relatively low, is easily obtained, and is killing three birds with one stone.The present invention adopts simultaneously
Feed is handled with the method that biology is quenched and curing is quenched, the quenched fermentation by microorganism of biology will be big in feed
Molecular substance is degraded to small-molecule substance easy to digest and absorb, makes feed be easier to be digested absorption, utilization rate is higher, and mitigates
The stomach of sea cucumber is born；Cure it is quenched feed softening, starch gelatinization can be made by humid heat treatment, be conducive to feed and shape, and improve
The water stability of feed, when application, will not dissolve at once, improve efficiency of feed utilization, reduce waste, while can kill feed
In pathogenic bacteria, make feed using safer.This method is easy to operate, cost is relatively low, and product obtained works well,
It is suitble to promote the use of.
Specific embodiment
The preparation of culture medium
A. potato dextrose medium: taking potato 200g, and 500mL water is added, boils 30min, filters, filtrate is taken to boil
Boiling, and 20g agar is added, it stirs to being completely dissolved, adds 20g glucose, be settled to 1L, 120 DEG C of high-temp steam sterilizings
20min。
The preparation of B.LB solid medium: beef extract 5g, peptone 10g, sodium chloride 10g is taken to be boiled with deionized water dissolving
15g agar is boiled and be added, is stirred to being completely dissolved, 1L is settled to, adjusts pH to 7.0~7.2,121 DEG C of high-temp steam sterilizings
20min。
The preparation of C.LB fluid nutrient medium: taking beef extract 5g, peptone 10g, sodium chloride 10g, with deionized water dissolving, and
It is settled to 1L, adjusts pH to 7.0~7.2,121 DEG C of high-temp steam sterilizing 20min.
The preparation of D.MRS broth bouillon: peptone 10g, beef extract 5g, yeast powder 4g, glucose 20g, tween are taken
801mL, dipotassium hydrogen phosphate 2g, sodium acetate 5g, Triammonium citrate 2g, magnesium sulfate 0.2g and manganese sulfate 0.05g, add deionized water
Agar powder 15g is boiled and is added in dissolution, is stirred to being completely dissolved, and 1L is settled to, and adjusts pH to 6.2, and 121 DEG C of high-temperature steams go out
Bacterium 20min.
The preparation of E.YPD fluid nutrient medium: taking yeast powder 10g, peptone 20g, glucose 20g, and it is water-soluble that deionization is added
Solution, is settled to 1L, 121 DEG C of high-temp steam sterilizing 20min.
The activation of strain
A. the activation of rhizopus: from the spore for the test tube slant picking rhizopus for being stored in 4 DEG C of refrigerators, it is inoculated into potato
On glucose test tube slant culture medium, the constant temperature incubation 48h at 28 DEG C, picking new life mycelium is transferred to potato glucose
On test tube slant culture medium, 48h is cultivated again at 28 DEG C.
B. the activation of Trichoderma: from the spore for the test tube slant picking Trichoderma for being stored in 4 DEG C of refrigerators, it is inoculated into potato
On glucose test tube slant culture medium, the constant temperature incubation 48h at 28 DEG C, picking new life mycelium is transferred to potato glucose
On test tube slant culture medium, 48h is cultivated again at 28 DEG C.
C. the activation of saccharomyces cerevisiae: from the test tube slant picking saccharomyces cerevisiae for being stored in 4 DEG C of refrigerators, it is inoculated into YPD liquid
In culture medium, constant temperature incubation for 24 hours, then is transferred in YPD fluid nutrient medium at 37 DEG C, is cultivated again for 24 hours at 37 DEG C
D. it the activation of bacillus subtilis: from the test tube slant picking bacillus subtilis for being stored in 4 DEG C of refrigerators, is inoculated into
On the test tube slant LB culture medium, constant temperature incubation for 24 hours, then is transferred on the test tube slant LB culture medium at 37 DEG C, at 37 DEG C again
Secondary culture is for 24 hours.
E. it the activation of bafillus natto: from the test tube slant picking bafillus natto for being stored in 4 DEG C of refrigerators, is inoculated into
On the test tube slant LB culture medium, constant temperature incubation for 24 hours, then is transferred on the test tube slant LB culture medium at 37 DEG C, at 37 DEG C again
Secondary culture is for 24 hours.
F. it the activation of Leuconostoc mesenteroides: from the test tube slant picking Leuconostoc mesenteroides for being stored in 4 DEG C of refrigerators, is inoculated into
On MRS broth bouillon, constant temperature incubation for 24 hours, then is transferred on MRS broth bouillon at 37 DEG C, is cultivated again at 37 DEG C
24h。
The expansion culture of strain
A. the expansion culture of rhizopus: the rhizopus mycelium through overactivation is inoculated on dregs of beans bran mass,
72h is cultivated at 28 DEG C.
B. the expansion culture of Trichoderma: the Trichoderma mycelium through overactivation is inoculated on dregs of beans bran mass,
72h is cultivated at 28 DEG C.
C. the expansion culture of saccharomyces cerevisiae: the saccharomyces cerevisiae through overactivation is inoculated into 50mL YPD fluid nutrient medium,
It is cultivated for 24 hours at 28 DEG C.
D. the expansion culture of bacillus subtilis: the LB liquid that the bacillus subtilis through overactivation is inoculated into 50mL is trained
It supports in base, constant temperature incubation is for 24 hours at 37 DEG C.
E. the expansion culture of bafillus natto: the LB liquid that the bafillus natto through overactivation is inoculated into 50mL is trained
It supports in base, constant temperature incubation is for 24 hours at 37 DEG C.
F. the Leuconostoc mesenteroides through overactivation the expansion culture of Leuconostoc mesenteroides: is inoculated into the MRS meat soup of 50mL
In culture medium, constant temperature incubation is for 24 hours at 37 DEG C.
Embodiment 1
A kind of preparation method for the holothurian feed adding scallop cylinder mould attachment, operating procedure are as follows:
(1) moss pre-processes: moss being cleaned with clear water, being placed in baking oven and being dried to water content is 10%, uses pulverizer
It crushes, and crosses 60 meshes, obtain moss powder；
(2) Zostera marina pre-processes: Zostera marina being cleaned with clear water, being placed in baking oven and being dried to water content is 10%, uses powder
Broken machine crushes, and crosses 60 meshes, obtains Zostera marina powder；
(3) scallop cylinder mould attachment pre-processes: scallop cylinder mould attachment being cleaned with clear water, is placed in baking oven and dries, is used
Pulverizer crushes, and obtains scallop cylinder mould attachment powder；
The scallop cylinder mould attachment includes algae and shell clast, the parts by weight of the algae and the shell clast
Than for 1:1, the algae includes Enteromorpha, sea lettuce, helictite algae and the boat-shaped algae that weight fraction ratio is 5:3:1:1,；
(4) basal feed prepare: by Zostera marina powder 200g made from moss powder 100g made from step (1), step (2),
The mixing of scallop cylinder mould attachment 450g made from step (3), stirs evenly, and basal feed is made；
(5) biology is quenched: being inoculated with the microorganism fungus kind of 7.5g in Xiang Suoshu basal feed, is cultivated, obtained at 30 DEG C
To fermented feed；
(6) granulating and forming: it is 10% that the fermented feed, which is placed in 50 DEG C of drying in oven to moisture, uses feed grinder
Break process is carried out, the finished product holothurian feed that partial size is 0.5mm is obtained.
Embodiment 2
A kind of preparation method for the holothurian feed adding scallop cylinder mould attachment, operating procedure are as follows:
(1) moss pre-processes: moss being cleaned with clear water, being placed in baking oven and being dried to water content is 10%, uses pulverizer
It crushes, and crosses 60 meshes, obtain moss powder；
(2) Zostera marina pre-processes: Zostera marina being cleaned with clear water, being placed in baking oven and being dried to water content is 10%, uses powder
Broken machine crushes, and crosses 60 meshes, obtains Zostera marina powder；
(3) scallop cylinder mould attachment pre-processes: scallop cylinder mould attachment being cleaned with clear water, is placed in baking oven and dries, is used
Pulverizer crushes, and obtains scallop cylinder mould attachment powder；
The scallop cylinder mould attachment includes algae and shell clast, the parts by weight of the algae and the shell clast
Than for 2:1, the algae includes Enteromorpha, sea lettuce, plumage algae and the knitting wool algae that weight fraction ratio is 5:3:1:1；
(4) basal feed prepare: by Zostera marina powder 400g made from moss powder 300g made from step (1), step (2),
The mixing of scallop cylinder mould attachment 650g made from step (3), stirs evenly, and basal feed is made；
(5) biology is quenched: being inoculated with the microorganism fungus kind of 40.5g in Xiang Suoshu basal feed, is cultivated, obtained at 30 DEG C
To fermented feed；
(6) granulating and forming: it is 10% that the fermented feed, which is placed in 50 DEG C of drying in oven to moisture, uses feed grinder
Break process is carried out, the finished product holothurian feed that partial size is 0.5mm is obtained.
Embodiment 3
A kind of preparation method for the holothurian feed adding scallop cylinder mould attachment, operating procedure are as follows:
(1) moss pre-processes: moss being cleaned with clear water, being placed in baking oven and being dried to water content is 10%, uses pulverizer
It crushes, and crosses 60 meshes, obtain moss powder；
(2) Zostera marina pre-processes: Zostera marina being cleaned with clear water, being placed in baking oven and being dried to water content is 10%, uses powder
Broken machine crushes, and crosses 60 meshes, obtains Zostera marina powder；
(3) scallop cylinder mould attachment pre-processes: scallop cylinder mould attachment being cleaned with clear water, is placed in baking oven and dries, is used
Pulverizer crushes, and obtains scallop cylinder mould attachment powder；
The scallop cylinder mould attachment includes algae and shell clast, the parts by weight of the algae and the shell clast
Than for 1:1, the algae includes Enteromorpha, sea lettuce, helictite algae and boat-shaped algae；
(4) basal feed prepare: by Zostera marina powder 300g made from moss powder 150g made from step (1), step (2),
The mixing of scallop cylinder mould attachment 550g made from step (3), it is another that 100g kudzu-vine root powder and 50g okra powder is added, it stirs evenly,
Basal feed is made；
(5) biology is quenched: being inoculated with the microorganism fungus kind of 20g in Xiang Suoshu basal feed, is cultivated, obtained at 30 DEG C
Fermented feed；
(6) granulating and forming: it is 10% that the fermented feed, which is placed in 50 DEG C of drying in oven to moisture, uses feed grinder
Break process is carried out, the finished product holothurian feed that partial size is 0.5mm is obtained.
Embodiment 4
A kind of preparation method for the holothurian feed adding scallop cylinder mould attachment, operating procedure are as follows:
(1) moss pre-processes: moss being cleaned with clear water, being placed in baking oven and being dried to water content is 10%, uses pulverizer
It crushes, and crosses 60 meshes, obtain moss powder；
(2) Zostera marina pre-processes: Zostera marina being cleaned with clear water, being placed in baking oven and being dried to water content is 10%, uses powder
Broken machine crushes, and crosses 60 meshes, obtains Zostera marina powder；
(3) scallop cylinder mould attachment pre-processes: scallop cylinder mould attachment being cleaned with clear water, is placed in baking oven and dries, is used
Pulverizer crushes, and obtains scallop cylinder mould attachment powder；
The scallop cylinder mould attachment includes algae and shell clast, the parts by weight of the algae and the shell clast
Than for 1:1, the algae includes Enteromorpha, sea lettuce, helictite algae and the boat-shaped algae that weight fraction ratio is 5:3:1:1；
(4) prepared by basal feed: by Zostera marina powder made from 100g parts of moss powder made from step (1), step (2)
The mixing of scallop cylinder mould attachment 450g made from 200g, step (3), stirs evenly, and basal feed is made；
(5) biology is quenched: the microorganism fungus kind through overactivation and expansion culture being inoculated into the basal feed, and is added
Enter 375ml sterile purified water, stir evenly, is placed in 24~48h of ferment at constant temperature in 30 DEG C of baking oven, obtains fermented feed；It is described
Microorganism fungus kind is rhizopus, Trichoderma, saccharomyces cerevisiae, bacillus subtilis, bafillus natto and Leuconostoc mesenteroides
Each 3.5g；
(6) granulating and forming: it is 10% that the fermented feed, which is placed in 50 DEG C of drying in oven to moisture, uses feed grinder
Break process is carried out, the finished product holothurian feed that partial size is 0.5mm is obtained.
Embodiment 5
A kind of preparation method for the holothurian feed adding scallop cylinder mould attachment, operating procedure are as follows:
(1) moss pre-processes: moss being cleaned with clear water, being placed in baking oven and being dried to water content is 10%, uses pulverizer
It crushes, and crosses 60 meshes, obtain moss powder；
(2) Zostera marina pre-processes: Zostera marina being cleaned with clear water, being placed in baking oven and being dried to water content is 10%, uses powder
Broken machine crushes, and crosses 60 meshes, obtains Zostera marina powder；
(3) scallop cylinder mould attachment pre-processes: scallop cylinder mould attachment being cleaned with clear water, is placed in baking oven and dries, is used
Pulverizer crushes, and obtains scallop cylinder mould attachment powder；
The scallop cylinder mould attachment includes algae and shell clast, the parts by weight of the algae and the shell clast
Than for 1:1, the algae includes Enteromorpha, sea lettuce, helictite algae and the boat-shaped algae that weight fraction ratio is 5:3:1:1；
(4) prepared by basal feed: by Zostera marina powder made from 100g parts of moss powder made from step (1), step (2)
The mixing of scallop cylinder mould attachment 450g made from 200g, step (3), it is another that 100g kudzu-vine root powder and 50g okra powder, stirring is added
Uniformly, basal feed is made；
(5) biology is quenched: the microorganism fungus kind through overactivation and expansion culture being inoculated into the basal feed, and is added
Enter 450ml sterile purified water, stir evenly, add culture medium, is placed in 24~48h of ferment at constant temperature in 30 DEG C of baking oven, obtains
Fermented feed；
The culture medium includes glucose 10g, beef extract 10g, peptone 6g, malt extract 10g, corn flour 10g, chlorination
Sodium 20g, magnesium sulfate 0.2g, potassium dihydrogen phosphate 0.5g, dipotassium hydrogen phosphate 0.3g；The microorganism fungus kind be rhizopus, Trichoderma,
Saccharomyces cerevisiae, bacillus subtilis, bafillus natto and each 3.5g of Leuconostoc mesenteroides；
(6) granulating and forming: it is 10% that the fermented feed, which is placed in 50 DEG C of drying in oven to moisture, uses feed grinder
Break process is carried out, the finished product holothurian feed that partial size is 0.5mm is obtained.
Embodiment 6
A kind of preparation method for the holothurian feed adding scallop cylinder mould attachment, operating procedure are as follows:
(1) moss pre-processes: moss being cleaned with clear water, being placed in baking oven and being dried to water content is 10%, uses pulverizer
It crushes, and crosses 60 meshes, obtain moss powder；
(2) Zostera marina pre-processes: Zostera marina being cleaned with clear water, being placed in baking oven and being dried to water content is 10%, uses powder
Broken machine crushes, and crosses 60 meshes, obtains Zostera marina powder；
(3) scallop cylinder mould attachment pre-processes: scallop cylinder mould attachment being cleaned with clear water, is placed in baking oven and dries, is used
Pulverizer crushes, and obtains scallop cylinder mould attachment powder；
The scallop cylinder mould attachment includes algae and shell clast, the parts by weight of the algae and the shell clast
Than for 1:1, the algae includes Enteromorpha, sea lettuce, helictite algae and the boat-shaped algae that weight fraction ratio is 5:3:1:1；
(4) prepared by basal feed: by Zostera marina powder made from 100g parts of moss powder made from step (1), step (2)
The mixing of scallop cylinder mould attachment 450g made from 200g, step (3), stirs evenly, and basal feed is made；
(5) biology is quenched: being inoculated with the microorganism fungus kind of 20g in Xiang Suoshu basal feed, is cultivated, obtained at 30 DEG C
Fermented feed；
(6) cure it is quenched: using efficient quality controling machine the fermented feed cure it is quenched, refining temperature be 95 DEG C,
Conditioning period is 35s, obtains cured feed；
(7) granulating and forming: it is 10% that the cured feed, which is placed in 50 DEG C of drying in oven to moisture, uses feed grinder
Break process is carried out, the finished product holothurian feed that partial size is 0.5mm is obtained.
Embodiment 7
A kind of preparation method for the holothurian feed adding scallop cylinder mould attachment, operating procedure are as follows:
(1) moss pre-processes: moss being cleaned with clear water, being placed in baking oven and being dried to water content is 10%, uses pulverizer
It crushes, and crosses 60 meshes, obtain moss powder；
(2) Zostera marina pre-processes: Zostera marina being cleaned with clear water, being placed in baking oven and being dried to water content is 10%, uses powder
Broken machine crushes, and crosses 60 meshes, obtains Zostera marina powder；
(3) scallop cylinder mould attachment pre-processes: scallop cylinder mould attachment being cleaned with clear water, is placed in baking oven and dries, is used
Pulverizer crushes, and obtains scallop cylinder mould attachment powder；
The scallop cylinder mould attachment includes algae and shell clast, the parts by weight of the algae and the shell clast
Than for 1:1, the algae includes Enteromorpha, sea lettuce, helictite algae and the boat-shaped algae that weight fraction ratio is 5:3:1:1；
(4) prepared by basal feed: by Zostera marina powder made from 100g parts of moss powder made from step (1), step (2)
The mixing of scallop cylinder mould attachment 450g made from 200g, step (3), it is another that 100g kudzu-vine root powder and 50g okra powder, stirring is added
Uniformly, basal feed is made；
(5) biology is quenched: the microorganism fungus kind through overactivation and expansion culture being inoculated into the basal feed, and is added
Enter 450ml sterile purified water, stir evenly, is placed in 24~48h of ferment at constant temperature in 30 DEG C of baking oven, obtains fermented feed；It is described
Microorganism fungus kind is rhizopus, Trichoderma, saccharomyces cerevisiae, bacillus subtilis, bafillus natto and Leuconostoc mesenteroides
Each 3.5g；
(6) cure it is quenched: using efficient quality controling machine the fermented feed cure it is quenched, refining temperature be 105 DEG C,
Conditioning period is 60s, obtains cured feed；
(7) granulating and forming: it is 10% that the cured feed, which is placed in 50 DEG C of drying in oven to moisture, uses feed grinder
Break process is carried out, the finished product holothurian feed that partial size is 0.5mm is obtained.
Embodiment 8
A kind of preparation method for the holothurian feed adding scallop cylinder mould attachment, operating procedure are as follows:
(1) moss pre-processes: moss being cleaned with clear water, being placed in baking oven and being dried to water content is 10%, uses pulverizer
It crushes, and crosses 60 meshes, obtain moss powder；
(2) Zostera marina pre-processes: Zostera marina being cleaned with clear water, being placed in baking oven and being dried to water content is 10%, uses powder
Broken machine crushes, and crosses 60 meshes, obtains Zostera marina powder；
(3) scallop cylinder mould attachment pre-processes: scallop cylinder mould attachment being cleaned with clear water, is placed in baking oven and dries, is used
Pulverizer crushes, and obtains scallop cylinder mould attachment powder；
The scallop cylinder mould attachment includes algae and shell clast, the parts by weight of the algae and the shell clast
Than for 1:1, the algae includes Enteromorpha, sea lettuce, helictite algae and the boat-shaped algae that weight fraction ratio is 5:3:1:1；
(4) basal feed prepare: by Zostera marina powder 300g made from moss powder 150g made from step (1), step (2),
The mixing of scallop cylinder mould attachment 550g made from step (3), stirs evenly, and basal feed is made；
(5) biology is quenched: being inoculated with the microorganism fungus kind of 20g in Xiang Suoshu basal feed, is cultivated, obtained at 30 DEG C
Fermented feed；
(6) it adds auxiliary agent: weighing 7.5g auxiliary agent, 20g bone tankage is added into auxiliary agent, adds 0.3g maize germ
Oil is added to after mixing in the fermented feed；
(7) granulating and forming: it is 10% that the fermented feed, which is placed in 50 DEG C of drying in oven to moisture, uses feed grinder
Break process is carried out, the finished product holothurian feed that partial size is 0.5mm is obtained.
Embodiment 9
A kind of preparation method for the holothurian feed adding scallop cylinder mould attachment, operating procedure are as follows:
(1) moss pre-processes: moss being cleaned with clear water, being placed in baking oven and being dried to water content is 10%, uses pulverizer
It crushes, and crosses 60 meshes, obtain moss powder；
(2) Zostera marina pre-processes: Zostera marina being cleaned with clear water, being placed in baking oven and being dried to water content is 10%, uses powder
Broken machine crushes, and crosses 60 meshes, obtains Zostera marina powder；
(3) scallop cylinder mould attachment pre-processes: scallop cylinder mould attachment being cleaned with clear water, is placed in baking oven and dries, is used
Pulverizer crushes, and obtains scallop cylinder mould attachment powder；
The scallop cylinder mould attachment includes algae and shell clast, the parts by weight of the algae and the shell clast
Than for 1:1, the algae includes Enteromorpha, sea lettuce, helictite algae and the boat-shaped algae that weight fraction ratio is 5:3:1:1；
(4) basal feed prepare: by Zostera marina powder 300g made from moss powder 150g made from step (1), step (2),
The mixing of scallop cylinder mould attachment 550g made from step (3), stirs evenly, and basal feed is made；
(5) biology is quenched: the microorganism fungus kind through overactivation and expansion culture being inoculated into the basal feed, and is added
Enter 500ml sterile purified water, stir evenly, is placed in 24~48h of ferment at constant temperature in 30 DEG C of baking oven, obtains fermented feed；It is described
Microorganism fungus kind is rhizopus, Trichoderma, saccharomyces cerevisiae, bacillus subtilis, bafillus natto and Leuconostoc mesenteroides
Each 3.5g；
(6) it adds auxiliary agent: weighing 7.5g auxiliary agent, 20g bone tankage is added into auxiliary agent, adds 0.3g maize germ
Oil is added to after mixing in the feed；The auxiliary agent includes mannan-oligosaccharides 3g, galactooligosacchariwith 3g, the dense element 1g of mandarin orange
And saccharicter-penin 0.5g.
(7) granulating and forming: it is 10% that the fermented feed, which is placed in 50 DEG C of drying in oven to moisture, uses feed grinder
Break process is carried out, the finished product holothurian feed that partial size is 0.5mm is obtained.
Embodiment 10
A kind of preparation method for the holothurian feed adding scallop cylinder mould attachment, operating procedure are as follows:
(1) moss pre-processes: moss being cleaned with clear water, being placed in baking oven and being dried to water content is 10%, uses pulverizer
It crushes, and crosses 60 meshes, obtain moss powder；
(2) Zostera marina pre-processes: Zostera marina being cleaned with clear water, being placed in baking oven and being dried to water content is 10%, uses powder
Broken machine crushes, and crosses 60 meshes, obtains Zostera marina powder；
(3) scallop cylinder mould attachment pre-processes: scallop cylinder mould attachment being cleaned with clear water, is placed in baking oven and dries, is used
Pulverizer crushes, and obtains scallop cylinder mould attachment powder；
The scallop cylinder mould attachment includes algae and shell clast, the parts by weight of the algae and the shell clast
Than for 1:1, the algae includes Enteromorpha, sea lettuce, helictite algae and the boat-shaped algae that weight fraction ratio is 5:3:1:1；
(4) basal feed prepare: by Zostera marina powder 300g made from moss powder 150g made from step (1), step (2),
The mixing of scallop cylinder mould attachment 550g made from step (3), stirs evenly, and basal feed is made；
(5) biology is quenched: being inoculated with the microorganism fungus kind of 20g in Xiang Suoshu basal feed, is cultivated, obtained at 30 DEG C
Fermented feed；
(6) cure it is quenched: using efficient quality controling machine the fermented feed cure it is quenched, refining temperature be 95 DEG C,
Conditioning period is 35s, obtains cured feed；
(7) it adds auxiliary agent: weighing 7.5g auxiliary agent, 40g bone tankage is added into auxiliary agent, adds 0.3g maize germ
Oil is added to after mixing in the fermented feed；
(8) granulating and forming: it is 10% that the fermented feed, which is placed in 50 DEG C of drying in oven to moisture, uses feed grinder
Break process is carried out, the finished product holothurian feed that partial size is 0.5mm is obtained.
Embodiment 11
A kind of preparation method for the holothurian feed adding scallop cylinder mould attachment, operating procedure are as follows:
(1) moss pre-processes: moss being cleaned with clear water, being placed in baking oven and being dried to water content is 10%, uses pulverizer
It crushes, and crosses 60 meshes, obtain moss powder；
(2) Zostera marina pre-processes: Zostera marina being cleaned with clear water, being placed in baking oven and being dried to water content is 10%, uses powder
Broken machine crushes, and crosses 60 meshes, obtains Zostera marina powder；
(3) scallop cylinder mould attachment pre-processes: scallop cylinder mould attachment being cleaned with clear water, is placed in baking oven and dries, is used
Pulverizer crushes, and obtains scallop cylinder mould attachment powder；
The scallop cylinder mould attachment includes algae and shell clast, the parts by weight of the algae and the shell clast
Than for 1:1, the algae includes Enteromorpha, sea lettuce, helictite algae and the boat-shaped algae that weight fraction ratio is 5:3:1:1；
(4) prepared by basal feed: by Zostera marina powder made from 150g parts of moss powder made from step (1), step (2)
The mixing of scallop cylinder mould attachment 550g made from 300g, step (3), it is another that 100g kudzu-vine root powder and 50g okra powder, stirring is added
Uniformly, basal feed is made；
(5) biology is quenched: the microorganism fungus kind through overactivation and expansion culture being inoculated into the basal feed, and is added
Enter 575ml sterile purified water, stir evenly, add culture medium, is placed in 24~48h of ferment at constant temperature in 30 DEG C of baking oven, obtains
Fermented feed；
The culture medium includes glucose 10g, beef extract 10g, peptone 6g, malt extract 10g, corn flour 10g, chlorination
Sodium 20g, magnesium sulfate 0.2g, potassium dihydrogen phosphate 0.5g, dipotassium hydrogen phosphate 0.3g,；The microorganism fungus kind is rhizopus, trichoderma
Bacterium, saccharomyces cerevisiae, bacillus subtilis, bafillus natto and each 3.5g of Leuconostoc mesenteroides；
(6) cure it is quenched: using efficient quality controling machine the fermented feed cure it is quenched, refining temperature be 100 DEG C,
Conditioning period is 50s, obtains cured feed；
(7) it adds auxiliary agent: weighing 7.5g auxiliary agent, 20g bone tankage is added into auxiliary agent, adds 0.3g maize germ
Oil is added to after mixing in the fermented feed；
The auxiliary agent includes mannan-oligosaccharides 3g, galactooligosacchariwith 3g, the dense element 1g and saccharicter-penin 0.5g of mandarin orange；
(8) granulating and forming: it is 10% that the fermented feed, which is placed in 50 DEG C of drying in oven to moisture, uses feed grinder
Break process is carried out, the finished product holothurian feed that partial size is 0.5mm is obtained.
Reference examples 1
A kind of preparation method for the holothurian feed adding scallop cylinder mould attachment, in operation without biological modulated
Matter step, and moisture content should reach 15% when drying, and remaining operation is the same as embodiment 4.
Reference examples 2
A kind of preparation method for the holothurian feed adding scallop cylinder mould attachment, the bacterium used in the quenched step of biology
Kind is Trichoderma, saccharomyces cerevisiae and bacillus subtilis, remaining operation is the same as embodiment 5.
Reference examples 3
A kind of preparation method for the holothurian feed adding scallop cylinder mould attachment, the bacterium used in the quenched step of biology
Kind is rhizopus, saccharomyces cerevisiae and bafillus natto, remaining operation is the same as embodiment 7.
Reference examples 4
A kind of preparation method for the holothurian feed adding scallop cylinder mould attachment, the bacterium used in the quenched step of biology
Kind is Trichoderma, rhizopus, saccharomyces cerevisiae, bacillus subtilis and lactic acid bacteria, remaining operation is the same as embodiment 9.
Reference examples 5
A kind of preparation method for the holothurian feed adding scallop cylinder mould attachment, in operation without overcuring tune
Matter, remaining operation is the same as embodiment 6.
Reference examples 6
A kind of preparation method for the holothurian feed adding scallop cylinder mould attachment, the tune used in curing quenched step
Matter temperature is 85~90 DEG C, conditioning period 90s, remaining operation is the same as embodiment 7.
Reference examples 7
A kind of preparation method for the holothurian feed adding scallop cylinder mould attachment, the tune used in curing quenched step
Matter temperature is 90~94 DEG C, conditioning period 60s, remaining operation is the same as embodiment 10.
Reference examples 8
A kind of preparation method for the holothurian feed adding scallop cylinder mould attachment, the tune used in curing quenched step
Matter temperature is 106~110 DEG C, conditioning period 50s, remaining operation is the same as embodiment 11.
Experimental example 1
Efficiency of feed utilization comparative experiments
Using holothurian feed made from the method in embodiment 1-5 and 7,9,11 as experimental group 1-8, with reference examples 1-4
In method made from holothurian feed 1-4 as a control group.Every group setting 6 parallel, and 10 sea cucumbers is each taken to be put into 40L in parallel
Bucket in cultivated, continuous culture two months at room temperature.A water is changed within every 4 days from the beginning, and keeps cultivating
With the salinity of water 1.023 ‰.Bait is launched while changing water every time, injected volume is 5g/ barrels；Continue in incubation into bucket
Oxygenation.Per two weeks to sea cucumber weight carry out one-shot measurement, and calculate each group average weight gain and feed coefficient (feed coefficient=
Feed consumption/gain in weight), it records 5 times altogether.
1 each group sea cucumber weight average growth pattern of table
As shown in Table 1, the balanced growth amplitude of experimental group 1-5 is above control group 1-4；In all processing, experimental group 5
Balanced growth amplitude highest, the balanced growth amplitude of control group 1 is minimum.It follows that biological modifier treatment is carried out to feed,
The growth of sea cucumber weight can be remarkably promoted.And when a variety of strains are used in mixed way, this facilitation effect, which is better than, to be only used
The effect of not several strains；Wherein, by rhizopus, Trichoderma, saccharomyces cerevisiae, bacillus subtilis, bafillus natto and
When six kinds of microorganism fungus kinds of Leuconostoc mesenteroides are used in combination, this gaining effect is best.
2 each group feed coefficient calculated result of table
As shown in Table 4, the feed coefficient of experimental group 1-5 is substantially less than control group 1-4.In the identical feelings of feed consumption
Under condition, increase weight more, feed coefficient is lower, shows that efficiency of feed utilization is higher.It follows that carrying out the quenched place of biology to feed
Reason, can promote the utilization rate of feed.
Experimental example 2
The experiment of feed water stability
Using holothurian feed made from the method in embodiment 1,5,7,11 as experimental group 1-4, with the side in reference examples 5-8
1-4, every group of sample weigh 10g and are placed in the bowl-shape sieve in the aperture 0.5mm holothurian feed made from method as a control group, and sieve is fixed
Above glass beaker inner wall, the sodium-chloride water solution of 1.023 ‰ concentration is added into beaker, liquid level is made not have sample just；
It placing the beaker in 28 DEG C of water-bath, the glass with hole pipe that the position inside beaker under sieve sets a 3mm is ventilated,
Ventilatory capacity is 40~60 bubbles per minute, is gently agitated for sample with this, the defeated and dispersed little particle got off of feed falls into burning from sieve pore
In cup, after 60min, the remaining feed on sieve pore is taken out, 105 DEG C of baking ovens is placed in and dries to constant weight.Every group of sample separately take 10g directly with
Drying to constant weight for same method, calculates its water content.Every group of sample does parallel test three times, takes its average value.Feed is in water
In stability indicate that calculation formula is as follows with rate of scattering and disappearing (%):
Rate of scattering and disappearing (%)=[m1 (1-x)-m2]/[m1 (1-x)] * 100
In formula: m1 --- the quality of Feed Sample, g；X --- moisture content, %；M2 --- the matter of the remaining feed after dry
Amount, g.
The rate of scattering and disappearing of 3 each group holothurian feed of table
As shown in Table 3, the feed rate of scattering and disappearing of experimental group 1~4 is substantially less than control group 1~4.In items such as time, temperature
In the case that part is all the same, rate of scattering and disappearing is higher, shows that feed dissolution is faster, i.e. the stability of feed in water is poorer.And it cures
Quenched temperature and time can all have an impact the water stability of feed.It follows that when 105 DEG C of refining temperature, quenched
When 35~60s of time, feed rate of scattering and disappearing obtained is minimum, water stability is best, and wherein with 100 DEG C of refining temperature, tune
Matter time 50s is optimum condition.
The embodiments described above only express several embodiments of the present invention, and the description thereof is more specific and detailed, but simultaneously
Limitations on the scope of the patent of the present invention therefore cannot be interpreted as.It should be pointed out that for those of ordinary skill in the art
For, without departing from the inventive concept of the premise, various modifications and improvements can be made, these belong to guarantor of the invention
Protect range.Therefore, the scope of protection of the patent of the invention shall be subject to the appended claims.
Claims (8)
1. a kind of preparation method for the holothurian feed for adding scallop cylinder mould attachment, which comprises the steps of:
(1) moss pre-processes: moss being cleaned with clear water, is placed in baking oven and is dried to water content less than 12%, with pulverizer powder
It is broken, and 60 meshes are crossed, obtain moss powder；
(2) Zostera marina pre-processes: Zostera marina being cleaned with clear water, is placed in baking oven and is dried to water content less than 12%, with crushing
Machine crushes, and crosses 60 meshes, obtains Zostera marina powder；
(3) scallop cylinder mould attachment pre-processes: scallop cylinder mould attachment being cleaned with clear water, is placed in baking oven and dries, with crushing
Machine crushes, and obtains scallop cylinder mould attachment powder；
The scallop cylinder mould attachment includes algae and shell clast, and the algae and the weight fraction ratio of the shell clast are
1~2:1, the algae include at least one of Enteromorpha, sea lettuce, helictite algae, boat-shaped algae, plumage algae or knitting wool algae；
(4) prepared by basal feed: by Zostera marina powder 20~40 made from 10~20 parts of moss powder made from step (1), step (2)
45~65 parts of scallop cylinder mould attachment mixing, stir evenly made from part, step (3), and basal feed is made；
(5) biology is quenched: it is inoculated with the microorganism fungus kind of 1~3% mass in Xiang Suoshu basal feed, is cultivated at 30 DEG C,
Obtain fermented feed；
The microorganism fungus kind be weight fraction ratio be 1:1:1:1:1:1 rhizopus, Trichoderma, saccharomyces cerevisiae, withered grass gemma
The mixture of bacillus, bafillus natto and Leuconostoc mesenteroides；
(6) granulating and forming: being placed in 50 DEG C of drying in oven to moisture less than 12% for the fermented feed, with feed grinder into
Row break process obtains the finished product holothurian feed that partial size is 0.5mm.
2. the preparation method of the holothurian feed of addition scallop cylinder mould attachment as described in claim 1, which is characterized in that described
The preparation process of basal feed further includes that addition parts by weight are 7~15 parts of kudzu-vine root powder and 3~8 parts of okra powder.
3. the preparation method of the holothurian feed of addition scallop cylinder mould attachment as described in claim 1, which is characterized in that described
The quenched method of biology is as follows in step (5): the microorganism fungus kind through overactivation and expansion culture is connect by 2% mass ratio
Sterile purified water is added into the basal feed, and in 50% ratio in kind, stirs evenly, is placed in constant temperature in 30 DEG C of baking oven
Ferment 24~48h, obtains fermented feed.
4. the preparation method of the holothurian feed of addition scallop cylinder mould attachment as claimed in claim 3, which is characterized in that described
The quenched method of biology is as follows in step (5): the microorganism fungus kind through overactivation and expansion culture is connect by 2% mass ratio
Sterile purified water is added into the powder, and in 50% ratio in kind, stirs evenly, adds culture medium, is placed in 30 DEG C
Baking oven in 24~48h of ferment at constant temperature, obtain fermented feed；The culture medium includes the ingredient of following parts by weight: glucose
10 parts, 10 parts of beef extract, 6 parts of peptone, 10 parts of malt extract, 10 parts of corn flour, 20 parts of sodium chloride, 0.2 part of magnesium sulfate, chlorination
0.5 part of calcium, 0.5 part of potassium dihydrogen phosphate, 0.3 part of dipotassium hydrogen phosphate.
5. the preparation method of the holothurian feed of addition scallop cylinder mould attachment as described in claim 1, which is characterized in that described
It further include curing quenched step between step (5) and the step (6), the curing is quenched method particularly includes: using efficient
Quality controling machine the fermented feed cure it is quenched, refining temperature be 95~105 DEG C, conditioning period be 35~60s, obtain ripe
Change feed.
6. the preparation method of the holothurian feed of addition scallop cylinder mould attachment as described in claim 1, which is characterized in that described
It further include addition additives step between step (5) and step (6), the adding method of the auxiliary agent is as follows: pressing the auxiliary agent 4~8
Quality again adds bone tankage into the auxiliary agent, adds the maize germ oil for accounting for the bone tankage quality 1.5%, mixes
It is added in the feed after closing uniformly.
7. the preparation method of the holothurian feed of addition scallop cylinder mould attachment as claimed in claim 6, which is characterized in that described
Auxiliary agent includes the ingredient of following parts by weight: 3 parts of mannan-oligosaccharides, 3 parts of galactooligosacchariwith, mandarin orange dense plain 1 part and saccharicter-penin 0.5
Part.
8. the holothurian feed of the method addition scallop cylinder mould attachment obtained according to claim 1~any one of 7.
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Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101971922A (en) * | 2010-07-15 | 2011-02-16 | 大连理工大学 | Environment-friendly sea cucumber streak feed and preparation method thereof |
| CN102283328A (en) * | 2010-12-23 | 2011-12-21 | 中国水产科学研究院黄海水产研究所 | Multifunctional compound micro-ecological preparation for culturing holothurian |
| CN104805155A (en) * | 2015-05-20 | 2015-07-29 | 大连大学 | Kelp oligosaccharide with functions of promoting trepang growth and stress resistance, and preparation method and application thereof |
| CN105567616A (en) * | 2016-03-22 | 2016-05-11 | 青岛东海药业有限公司 | Microbial composition and application thereof |
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2016
- 2016-09-05 CN CN201610802001.7A patent/CN106417924B/en not_active Expired - Fee Related
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101971922A (en) * | 2010-07-15 | 2011-02-16 | 大连理工大学 | Environment-friendly sea cucumber streak feed and preparation method thereof |
| CN102283328A (en) * | 2010-12-23 | 2011-12-21 | 中国水产科学研究院黄海水产研究所 | Multifunctional compound micro-ecological preparation for culturing holothurian |
| CN104805155A (en) * | 2015-05-20 | 2015-07-29 | 大连大学 | Kelp oligosaccharide with functions of promoting trepang growth and stress resistance, and preparation method and application thereof |
| CN105567616A (en) * | 2016-03-22 | 2016-05-11 | 青岛东海药业有限公司 | Microbial composition and application thereof |
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