CN104737814A - Rope type mycelia and production method thereof - Google Patents
Rope type mycelia and production method thereof Download PDFInfo
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- CN104737814A CN104737814A CN201510157253.4A CN201510157253A CN104737814A CN 104737814 A CN104737814 A CN 104737814A CN 201510157253 A CN201510157253 A CN 201510157253A CN 104737814 A CN104737814 A CN 104737814A
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Abstract
The invention relates to rope type mycelia and a production method thereof and belongs to the field of edible fungus mycelia. A nutrient solution is prepared, wound ropes are placed in the nutrient solution to be soaked, totally soaked bobbins are directly arranged in ethene or propylene bags, subjected to high pressure or atmospheric pressure disinfection, and placed in a clean environment to be cooled, inoculation is conducted under aseptic conditions when the temperature of the mycelium bags decreases to 25 DEG C-30 DEG C, the inoculated mycelium bags are placed in a culture room at the temperature of 24 DEG C-26 DEG C to be cultured, and after the mycelium bags are full of mycelia and ripe for 7-10 days, the mycelium bags are stored in the environment of 4 DEG C. Quantitative mycelia are obtained, the mycelium bags conduct spawn running consistently, and management is facilitated; the rope type mycelia have simple requirements for the forms of the package bags, production efficiency is improved, and production cost is reduced. By the adoption of inoculation of the rope type mycelia, rapid and quantitative inoculation can be achieved, and the mycelium bags have a high spawn running speed and a low pollution rate.
Description
Technical field
The invention belongs to edible fungus species field, refer to especially and utilize the cotton rope of good water absorption for hypha of edible fungus growth substrate.
Background technology
General mycelial growth is with regard to called after " so-and-so bacterial classification " in certain matrix at present, and naming method is: culture matrix type+bacterial classification, and as tree fungus, or the matrix of mycelial growth is wood chip, be then called " Saw-dust ".
The bacterial classification that current production uses is Saw-dust, tree fungus and liquid spawn mainly.The anti-polygamy of Saw-dust is strong but productive labor intensity greatly, costly, is easily polluted and the cycle is long, the feature that output is also unstable; The convenient inoculation of tree fungus, cell age is unanimous between the higher and lower levels can shorten mycelium growing period but branch is invaded the bubble time and is difficult to grasp, perishable, easily acid, and the loaded down with trivial details labour intensity of bagging process is large; Liquid spawn can be increased work efficiency, suitable batch production use but require to produce and operating condition higher, require aseptic in the whole process made, but also need special technical staff and certain instrument etc., and not easily storage, unfavorable transport.
Current Edible Fungi is produced as many with decentralized peasant household, therefore main based on Saw-dust and tree fungus, is all main material with timber.Along with the increasing of environmental protection dynamics, Wood cut presents downward trend year by year, constrains the development of edible mushroom to a certain extent.
Summary of the invention
The invention provides a kind of cotton rope type bacterial classification and production method thereof, to solve Saw-dust, easy pollution that tree fungus and liquid spawn exist and the cycle long, output is unstable, and the consumption of timber is large, the problem of not easily storage, unfavorable transport.
" cotton rope type bacterial classification " the i.e. of the present invention growth substrate of mycelia is cotton rope, is a kind of novel cultural hypha matrix.
The technical scheme that the present invention takes is obtained by the following step:
(1) nutrient solution is prepared, formula: potato 300g, glucose 20g, peptone 2g, potassium dihydrogen phosphate 2g, magnesium sulfate 1g, water 1000ml; Concrete operations:
The potato of precise is cut into the sheet that thickness is about 0.3cm, water is put into pot and is heated to boiling, the potato block cut is put into boiling water, boil 15min, filtration is left and taken filtrate and is continued heating, treats that liquid seethes with excitement again and glucose, peptone, potassium dihydrogen phosphate, magnesium sulfate to be added successively in liquid; Complete nutrient solution is poured in container for subsequent use;
(2) cotton rope process: the specification according to need of production and bacterium bag processes cotton rope, require that every bag of inner cord is one, cotton rope is wrapped in the plastic shaft of diameter 0.5 centimetre, the cotton rope wound is put into nutrient solution soak, soak time is determined according to the diameter of bobbin, the bobbin of diameter 10cm, soaks 30 seconds;
(3) pack: be directly hinged with in ethene or propylene bag by the saturating bobbin of immersion, bacterium bag size is determined according to production requirement, after pack, heavy 1.25-1.5kg, stays bacterium bag opening part, then seals by cotton rope head;
(4) sterilizing requires: adopt high pressure or normal-pressure sterilization, sterilizing parameter: normal-pressure sterilization 100 DEG C maintains 8-10 hour; Or autoclaving 121 DEG C, 2 hours;
(5) cooling and inoculation: will the bacterium bag of sterilizing be completed, and cool under being placed on clean environment, and when bacterium bag temperature is down to 25-30 DEG C, aseptically inoculate;
(6) cultivate: will complete the bacterium bag connecing bacterium, cultivate in the culturing room being positioned over 24-26 DEG C, after bacterium Bao Faman mycelia, after-ripening 7-10 days, places it in preservation under the environment of 4 DEG C.
The present invention is with whole cotton rope after immersion for mycelial growth matrix, and the cotton rope after immersion is nutritious, and the good cotton rope quality of water imbibition is loosened, good permeability, and mycelial growth rate is fast, and production technology is simple; Cotton rope type bacterial classification adopts " trying to make a match " formula mode to inoculate, and inoculation speed is fast, and bacterial classification and bacterium bag contact area are greater than liquid spawn, send out bacterium speed fast, the hyphae length that the cotton rope of equal length contains is roughly the same, can realize quantitative bacterial classification, it is consistent that bacterium sent out by the bacterium bag that connects, and is convenient to management; The pack mode of cotton rope bacterial classification to connect bacterium bag requires that simply, can save plunger technique for agaric fungus bag, the bacterium bags such as Xingbao mushroom save the link that the collar is added a cover, and enhance productivity, and reduces production cost.Adopt cotton rope strain inoculation, can realize inoculating fast, quantitatively, it is fast that bacterium speed sent out by the bacterium bag that connects, and pollution rate is low.
Embodiment
" cotton rope type bacterial classification " the i.e. of the present invention growth substrate of mycelia is cotton rope, is a kind of novel cultural hypha matrix.
Embodiment 1
Obtained by the following step:
(1) nutrient solution is prepared, formula: potato 300g, glucose 20g, peptone 2g, potassium dihydrogen phosphate 2g, magnesium sulfate 1g, water 1000ml; Concrete operations:
The potato of precise is cut into the sheet that thickness is about 0.3cm, water is put into pot and is heated to boiling, the potato block cut is put into boiling water, boil 15min, filtration is left and taken filtrate and is continued heating, treats that liquid seethes with excitement again and glucose, peptone, potassium dihydrogen phosphate, magnesium sulfate to be added successively in liquid; Complete nutrient solution is poured in container for subsequent use;
(2) cotton rope process: the specification according to need of production and bacterium bag processes cotton rope, require that every bag of inner cord is one, cotton rope is wrapped in the plastic shaft of diameter 0.5 centimetre, the cotton rope wound is put into nutrient solution soak, soak time is determined according to the diameter of bobbin, the bobbin of diameter 10cm, soaks 30 seconds;
(3) pack: be directly hinged with in ethene or propylene bag by the saturating bobbin of immersion, bacterium bag size is determined according to production requirement, and bacterium bag specification is 17cm × 35cm, after pack, heavy 1.25kg, stays bacterium bag opening part, then seals by cotton rope head;
(4) sterilizing requires: adopt high pressure or normal-pressure sterilization, sterilizing parameter: normal-pressure sterilization 100 DEG C maintains 8 hours; Or autoclaving 121 DEG C, 2 hours;
(5) cooling and inoculation: will the bacterium bag of sterilizing be completed, and cool under being placed on clean environment, and when bacterium bag temperature is down to 25 DEG C, aseptically inoculate;
(6) cultivate: will complete the bacterium bag connecing bacterium, cultivate in the culturing room being positioned over 24 DEG C, after bacterium Bao Faman mycelia, after-ripening 7 days, places it in preservation under the environment of 4 DEG C.
Embodiment 2
Obtained by the following step:
(1) nutrient solution is prepared, formula: potato 300g, glucose 20g, peptone 2g, potassium dihydrogen phosphate 2g, magnesium sulfate 1g, water 1000ml; Concrete operations:
The potato of precise is cut into the sheet that thickness is about 0.3cm, water is put into pot and is heated to boiling, the potato block cut is put into boiling water, boil 15min, filtration is left and taken filtrate and is continued heating, treats that liquid seethes with excitement again and glucose, peptone, potassium dihydrogen phosphate, magnesium sulfate to be added successively in liquid; Complete nutrient solution is poured in container for subsequent use;
(2) cotton rope process: the specification according to need of production and bacterium bag processes cotton rope, require that every bag of inner cord is one, cotton rope is wrapped in the plastic shaft of diameter 0.5 centimetre, the cotton rope wound is put into nutrient solution soak, soak time is determined according to the diameter of bobbin, the bobbin of diameter 10cm, soaks 30 seconds;
(3) pack: be directly hinged with in ethene or propylene bag by the saturating bobbin of immersion, bacterium bag size is determined according to production requirement, and bacterium bag specification is 17cm × 35cm, after pack, heavy 1.4kg, stays bacterium bag opening part, then seals by cotton rope head;
(4) sterilizing requires: adopt high pressure or normal-pressure sterilization, sterilizing parameter: normal-pressure sterilization 100 DEG C maintains 9 hours; Or autoclaving 121 DEG C, 2 hours;
(5) cooling and inoculation: will the bacterium bag of sterilizing be completed, and cool under being placed on clean environment, and when bacterium bag temperature is down to 28 DEG C, aseptically inoculate;
(6) cultivate: will complete the bacterium bag connecing bacterium, cultivate in the culturing room being positioned over 25 DEG C, after bacterium Bao Faman mycelia, after-ripening 9 days, places it in preservation under the environment of 4 DEG C.
Embodiment 3
Obtained by the following step:
(1) nutrient solution is prepared, formula: potato 300g, glucose 20g, peptone 2g, potassium dihydrogen phosphate 2g, magnesium sulfate 1g, water 1000ml; Concrete operations:
The potato of precise is cut into the sheet that thickness is about 0.3cm, water is put into pot and is heated to boiling, the potato block cut is put into boiling water, boil 15min, filtration is left and taken filtrate and is continued heating, treats that liquid seethes with excitement again and glucose, peptone, potassium dihydrogen phosphate, magnesium sulfate to be added successively in liquid; Complete nutrient solution is poured in container for subsequent use;
(2) cotton rope process: the specification according to need of production and bacterium bag processes cotton rope, require that every bag of inner cord is one, cotton rope is wrapped in the plastic shaft of diameter 0.5 centimetre, the cotton rope wound is put into nutrient solution soak, soak time is determined according to the diameter of bobbin, the bobbin of diameter 10cm, soaks 30 seconds;
(3) pack: be directly hinged with in ethene or propylene bag by the saturating bobbin of immersion, bacterium bag size is determined according to production requirement, and bacterium bag specification is 17cm × 35cm, after pack, heavy 1.5kg, stays bacterium bag opening part, then seals by cotton rope head;
(4) sterilizing requires: adopt high pressure or normal-pressure sterilization, sterilizing parameter: normal-pressure sterilization 100 DEG C maintains 10 hours; Or autoclaving 121 DEG C, 2 hours;
(5) cooling and inoculation: will the bacterium bag of sterilizing be completed, and cool under being placed on clean environment, and when bacterium bag temperature is down to 30 DEG C, aseptically inoculate;
(6) cultivate: will complete the bacterium bag connecing bacterium, cultivate in the culturing room being positioned over 26 DEG C, after bacterium Bao Faman mycelia, after-ripening 10 days, places it in preservation under the environment of 4 DEG C.
Inoculation can access PDA bacterial classification or liquid spawn.
Use: the cotton rope type bacterial classification of preservation is taken out, under 24-26 DEG C of condition, activate 24 hours.The end of a thread is connected on inoculation device, connect probe to carry out up and down moving and cotton rope bacterial classification directly accessed band to connect bacterium bacterium bag inner, probe penetrates bacterium and wraps in the end of a thread that about 0.5cm is left in bacterium bag bottom, and go out apart from the bacterium side of wrapping 0.5cm and cut off by cotton rope, the end of a thread utilizing bacterium bag two to leave seals.Cultivate under inoculated bacterium bag is moved to 24-26 DEG C of environment, culturing room's temperature high humility is low, and the end of a thread is air-dry very soon, can not pollute inoculation bacterium bag.
Following table is Saw-dust, tree fungus, purseful time of liquid spawn and cotton rope type bacterial classification and pollution rate contrast:
As seen from the above table, it is long that cotton rope type bacterial classification avoids Saw-dust growth cycle respectively, and tree fungus branch soaking technology is complicated, liquid spawn poor stability and the shortcoming of not easily transporting and preserving; Combine the anti-polygamy of Saw-dust strong, tree fungus inoculation is convenient and swift, and liquid spawn sends out multiple advantages such as fast, the quantitative inoculation of bacterium speed, has wide market prospects.
Claims (2)
1. a cotton rope type bacterial classification, it is characterized in that being obtained by the following step:
(1) nutrient solution is prepared, formula: potato 300g, glucose 20g, peptone 2g, potassium dihydrogen phosphate 2g, magnesium sulfate 1g, water 1000ml; Concrete operations:
The potato of precise is cut into the sheet that thickness is about 0.3cm, water is put into pot and is heated to boiling, the potato block cut is put into boiling water, boil 15min, filtration is left and taken filtrate and is continued heating, treats that liquid seethes with excitement again and glucose, peptone, potassium dihydrogen phosphate, magnesium sulfate to be added successively in liquid; Complete nutrient solution is poured in container for subsequent use;
(2) cotton rope process: the specification according to need of production and bacterium bag processes cotton rope, require that every bag of inner cord is one, cotton rope is wrapped in the plastic shaft of diameter 0.5 centimetre, the cotton rope wound is put into nutrient solution soak, soak time is determined according to the diameter of bobbin, the bobbin of diameter 10cm, soaks 30 seconds;
(3) pack: be directly hinged with in ethene or propylene bag by the saturating bobbin of immersion, bacterium bag size is determined according to production requirement, after pack, heavy 1.25-1.5kg, stays bacterium bag opening part, then seals by cotton rope head;
(4) sterilizing requires: adopt high pressure or normal-pressure sterilization, sterilizing parameter: normal-pressure sterilization 100 DEG C maintains 8-10 hour; Or autoclaving 121 DEG C, 2 hours;
(5) cooling and inoculation: will the bacterium bag of sterilizing be completed, and cool under being placed on clean environment, and when bacterium bag temperature is down to 25-30 DEG C, aseptically inoculate;
(6) cultivate: will complete the bacterium bag connecing bacterium, cultivate in the culturing room being positioned over 24-26 DEG C, after bacterium Bao Faman mycelia, after-ripening 7-10 days, places it in preservation under the environment of 4 DEG C.
2. the production method of cotton rope type bacterial classification as claimed in claim 1, is characterized in that comprising the following steps:
(1) nutrient solution is prepared, formula: potato 300g, glucose 20g, peptone 2g, potassium dihydrogen phosphate 2g, magnesium sulfate 1g, water 1000ml; Concrete operations:
The potato of precise is cut into the sheet that thickness is about 0.3cm, water is put into pot and is heated to boiling, the potato block cut is put into boiling water, boil 15min, filtration is left and taken filtrate and is continued heating, treats that liquid seethes with excitement again and glucose, peptone, potassium dihydrogen phosphate, magnesium sulfate to be added successively in liquid; Complete nutrient solution is poured in container for subsequent use;
(2) cotton rope process: the specification according to need of production and bacterium bag processes cotton rope, require that every bag of inner cord is one, cotton rope is wrapped in the plastic shaft of diameter 0.5 centimetre, the cotton rope wound is put into nutrient solution soak, soak time is determined according to the diameter of bobbin, the bobbin of diameter 10cm, soaks 30 seconds;
(3) pack: be directly hinged with in ethene or propylene bag by the saturating bobbin of immersion, bacterium bag size is determined according to production requirement, after pack, heavy 1.25-1.5kg, stays bacterium bag opening part, then seals by cotton rope head;
(4) sterilizing requires: adopt high pressure or normal-pressure sterilization, sterilizing parameter: normal-pressure sterilization 100 DEG C maintains 8-10 hour; Or autoclaving 121 DEG C, 2 hours;
(5) cooling and inoculation: will the bacterium bag of sterilizing be completed, and cool under being placed on clean environment, and when bacterium bag temperature is down to 25-30 DEG C, aseptically inoculate;
(6) cultivate: will complete the bacterium bag connecing bacterium, cultivate in the culturing room being positioned over 24-26 DEG C, after bacterium Bao Faman mycelia, after-ripening 7-10 days, places it in preservation under the environment of 4 DEG C.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201510157253.4A CN104737814A (en) | 2015-04-06 | 2015-04-06 | Rope type mycelia and production method thereof |
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| CN201510157253.4A CN104737814A (en) | 2015-04-06 | 2015-04-06 | Rope type mycelia and production method thereof |
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| CN104737814A true CN104737814A (en) | 2015-07-01 |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112400609A (en) * | 2020-11-25 | 2021-02-26 | 东平县科海菌业有限公司 | Edible fungus strain culture medium containing air-dried branch strains and preparation method |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4021305A (en) * | 1976-05-19 | 1977-05-03 | G. D. Searle & Co. | String culture inoculum |
| CN85107763A (en) * | 1985-10-29 | 1987-05-06 | 北京市食品研究所 | A kind of preparation method of edible fungus species |
| CN101104850A (en) * | 2007-06-20 | 2008-01-16 | 彭泽福 | Technique for producing cloth-shape and sheet-like edible mushroom culture |
-
2015
- 2015-04-06 CN CN201510157253.4A patent/CN104737814A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4021305A (en) * | 1976-05-19 | 1977-05-03 | G. D. Searle & Co. | String culture inoculum |
| CN85107763A (en) * | 1985-10-29 | 1987-05-06 | 北京市食品研究所 | A kind of preparation method of edible fungus species |
| CN101104850A (en) * | 2007-06-20 | 2008-01-16 | 彭泽福 | Technique for producing cloth-shape and sheet-like edible mushroom culture |
Non-Patent Citations (3)
| Title |
|---|
| 张传忠 等: "竹木签菌种的制作与使用", 《食用菌》 * |
| 张莆安 等: "香菇木耳竹签原种的制作及使用", 《种菇新技术荟萃》 * |
| 梁尤金: "竹签菌种快速制备法", 《农家科技》 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112400609A (en) * | 2020-11-25 | 2021-02-26 | 东平县科海菌业有限公司 | Edible fungus strain culture medium containing air-dried branch strains and preparation method |
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Application publication date: 20150701 |
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