A kind of seed treatment containing bacillus subtilis and its production and use
Technical field
The present invention relates to a kind of seed treatment, at more particularly to a kind of seed containing bacillus subtilis and ethiprole
Manage agent and preparation method thereof and associated uses.The invention belongs to technical field of pesticide.
Background technology
In modern agricultural production, it is preventing and treating subterranean pest-insect and farming to carry out chemicals treatment to seed using kind of clothing inorganic agent
One of thing soil biography, method of economical and convenient of seed-borne disease.Seed treatment is a kind of is used at crop or other plant seed
Reason, there are the pesticidal preparations of film forming characteristics, it is allowed to seed normally the moisture needed for germination and air by, contained agricultural chemicals and
The materials such as seed manure slowly discharge, and can kill subterranean pest-insect, seedling stage insect, have preventing and treating sprout term disease and systemic disease, carry
High percentage of seedgermination, promotion seedling healthy growth and other effects.
Suspension seed-coating agent is one kind of seed treatment, is by pesticide original medicine, film forming agent, wetting agent, dispersant, thickening
Agent, colouring agent, filler and water manufactured a kind of unit for uniform suspension of flowable stabilization through wet pulverizing.Due to its user
Just, safety, received much attention in the seed coat agent of numerous kinds.
At present, the demand of the annual crop seeds in China is about 7,000,000 tons, wherein about 3,500,000 tons of commodity seed amount, is done
Cotton seeds account for 10% or so, it is seen that the market demand of two kinds of coating agents is huge, and prospect is very wide.
The content of the invention
The invention aims to provide a kind of seed treatment of pesticide-germicide.
Based on this purpose, the technical solution adopted by the present invention is:
A kind of seed treatment, its effective ingredient are ethiprole(Fipronil)And bacillus subtilis
(Brevibacterium).
In the present invention, it is preferred to, in the seed treatment content of bacillus subtilis be 20~200,000,000,000 spores/
Gram, by weight percentage, the content of ethiprole is 1~20%.
In one embodiment of the invention, the seed treatment is Waterless Seed Dressing agent, containing bacillus subtilis 20~
200000000000 spores/g of formulation, by weight percentage, also containing ethiprole 1~20%, wetting agent 2.0~6.0%, dispersant 2.0
~8.0%, film forming agent 0.5~4.0%, remaining supplies 100% by filler.
Preferably, the wetting agent in the Waterless Seed Dressing agent is lauryl sodium sulfate, neopelex, pull open powder
Or more than one or both of wetting and penetrating agent T;The dispersant is sodium lignin sulfonate, di-2-ethylhexylphosphine oxide bitter edible plant sodium sulfonate
(NNO), naphthalenesulfonate formaldehyde condensation compound or methyl naphthalene sulfonic acid sodium formaldehyde condensation products(MF)One or both of more than.
Preferably, the film forming agent in the Waterless Seed Dressing agent is sodium cellulose glycolate, hydroxymethyl cellulose or polyvinyl alcohol
One or both of more than.
Preferably, the filler in the Waterless Seed Dressing agent is in diatomite, bentonite, white carbon, fine particle calcium carbonate, kaolin or clay
One or more.
In another embodiment of the invention, the seed treatment is suspension seed-coating agent, containing bacillus subtilis
20~200,000,000,000 spores/g of formulation, by weight percentage, also containing ethiprole 1~20%, wetting agent 2.0~6.0%, disperse
Agent 2.0~8.0%, film forming agent 0.5~4.0%, warning coloration 0.5~4.0%, thickener 0.1~0.6%, antifreezing agent 1.0~4.0%,
Preservative 0.1~1.0%, defoamer 0.1~1.0%, surplus are distilled water.
Preferably, in the suspension seed-coating agent, film forming agent is sodium carboxymethylcellulose, sodium hydroxyethlcellulose, carboxylic propyl group
It is more than one or both of sodium cellulosate, acrylic acid sodium, polyethylene glycol or polyvinylpyrrolidone.
Preferably, in the suspension seed-coating agent, dispersant is alkyl phenolic group polyethenoxy ether sulphonate, polycarboxylate, phosphorus
Hydrochlorate, sulfate, polyoxyethylene polyoxy propylene ether block copolymers, alkyl phenolic group polyoxyethylene groups phosphate, NNO, diffusant
It is more than one or both of MF or fatty alcohol ethylene oxide adduct sulfonate.
Preferably, in the suspension seed-coating agent, wetting agent is alkyl phenolic group polyoxyethylene ether, lauryl alcohol polyethylene glycol oxide base
Ether sodium sulfate, Negel, alkylsulfonate, alkyl naphthalene formaldehyde condensate sulfonates, polyoxyethylene sulfonated hydrocarbon, fat
Alcohol ethylene oxide adduct sulfonate, alkylphenol formaldehyde condensation product, ethylene oxide adduct sulfonate, APES
It is more than one or both of formaldehyde condensation products or polyoxyethylene polyoxy propylene ether block copolymers.
Preferably, in the suspension seed-coating agent, warning coloration is one kind in acid scarlet, water-based rhodo, basic rhodamine
It is or two or more.
Preferably, in the suspension seed-coating agent, thickener is one kind in xanthans, gelatin, Arabic gum, aluminium-magnesium silicate
It is or several.
Preferably, in the suspension seed-coating agent, antifreezing agent is ethylene glycol, propane diols, glycerine, urea, ammonium sulfate, chlorination
It is more than one or both of sodium, calcium chloride.
In the suspension seed-coating agent, preservative is paraformaldehyde.
In the suspension seed-coating agent, defoamer is organic silicon defoamer.
The seed treatment of the present invention is the biology formed with two kinds of effective ingredient compoundings of bacillus subtilis and ethiprole
Seed treatment, have efficiently, the characteristics of safety, environment compatibility are good, can pesticide-germicide, the ground of seed is endangered available for preventing and treating
Lower insect, growth period sucking pest and plurality of plant diseases, seed standardization is advantageously implemented, meets modern agriculture with high efficiency
Requirement.
Bacillus subtilis is a kind of new microbial source biological pesticide.Nutrition and space can be formed with other harmful bacterias
The competition in site, the surfactin with antibacterial, the effect of antiviral and biosurfactant can be produced.Withered grass gemma
Secondary metabolites caused by bacillus is harmful to pathogen archespore wall to other and produces dissolution, makes the cell of harmful levels of pathogens
Wall perforation, deformity etc..In addition, bacillus subtilis is as microorganism mushroom, additionally it is possible to promotes plant growth.
Ethiprole is a kind of Phenylpyrazole insecticides, and can be not only used for seed treatment can carry out foliar spray, insecticidal spectrum again
Extensively, to insect based on stomach poison function, have concurrently and tag and certain systemic action, it, which imposes on soil, can effectively prevent and treat corn root leaf
First, wireworm and cutworm.
Bacillus subtilis and ethiprole compatibility do seed treatment, and both mutually cooperate with, can be simultaneously from two differences
Target acted on, comprehensive protection is provided for seed, its Environmental compatibility is good, and toxicity is low, to biologies such as people's storage, natural enemies
Safety, belong to the biological seed inorganic agent of broad-spectrum high efficacy.The seed treatment can be made into Waterless Seed Dressing agent, suspension seed-coating agent, pass through
Seed dressing or seed pelleting, for preventing and treating the subterranean pest-insects such as the crop such as corn, wheat, rice grub, wireworm, mole cricket;Simultaneously
The multiple diseases such as the droop of crop, banded sclerotial blight, full rot, rice blast, gray mold, powdery mildew, bacterial wilt can be played anti-
Control effect.
Embodiment
The invention will now be further described with reference to specific embodiments, advantages of the present invention and feature will be with description and
It is apparent.But these embodiments are only exemplary, do not form any restrictions to the scope of the present invention.People in the art
Member to the details and form of technical solution of the present invention it should be understood that can enter without departing from the spirit and scope of the invention
Row modifications or substitutions, but these modifications and replacement are each fallen within protection scope of the present invention.
Embodiment 1, the preparation of Waterless Seed Dressing agent
Embodiment 1-1
Bacillus subtilis starter medicine(1000000000000 spores/gram)0.2kg, ethiprole(Folding hundred)1kg, wetting agent pull open powder
2.0kg, dispersant NNO4.0kg, film forming agent hydroxymethyl cellulose 0.5kg, separately supply 100kg with kaolin, and each component mixing is equal
Through air-flow crushing after even, the Waterless Seed Dressing agent 100kg of the living spores of 1% ethiprole 2,000,000,000/gram bacillus subtilis can be made into.
Embodiment 1-2
Bacillus subtilis starter medicine(1000000000000 spores/gram)10kg, ethiprole(Folding hundred)10kg, wetting agent dodecyl sulphur
Sour sodium 4.0kg, dispersant sodium lignin sulfonate 6.0kg, film forming agent sodium cellulose glycolate 2.0kg, are separately supplied with kaolin
100kg, through air-flow crushing after each component is well mixed, it can be made into the living spores of 10% ethiprole 100,000,000,000/gram bacillus subtilis
Waterless Seed Dressing agent 100kg.
Embodiment 1-3
Bacillus subtilis starter medicine(1000000000000 spores/gram)20kg, ethiprole(Folding hundred)20kg, wetting agent detergent alkylate
Sodium sulfonate 4.0kg, dispersant sodium lignin sulfonate 6.0kg, film forming agent sodium cellulose glycolate 2.0kg, are separately supplied with fine particle calcium carbonate
100kg, through air-flow crushing after each component is well mixed, it can be made into the living spores of 20% ethiprole 200,000,000,000/gram bacillus subtilis
Waterless Seed Dressing agent 100kg.
The preparation of embodiment 2, suspension seed-coating agent
Embodiment 2-1
Bacillus subtilis starter medicine(1000000000000 spores/gram)0.2kg, ethiprole(Folding hundred)1kg, wetting agent laruyl alcohol polyoxy
Vinyl ethers sodium sulphate 3.0kg, dispersant alkyl phenolic group polyoxyethylene groups phosphate 6.0kg, film forming agent sodium cellulose glycolate
2.0kg, warning coloration are acid scarlet 1.0kg, thickener xanthans 0.2kg, antifreeze glycol 4.0kg, preservative poly first
Aldehyde 0.3kg, organic silicon defoamer 0.2kg, separately supplies 100kg with water, is well mixed and is crushed through ball milling, sand milling, be scattered, can made
Into the aqueous suspension agent 100kg of 1% 2,000,000,000 living spores of ethiprole/g bacillus subtilises.
Embodiment 2-2
Bacillus subtilis starter medicine(1000000000000 spores/gram)10kg, ethiprole(Folding hundred)10kg, wetting agent dodecyl sulphur
Sour sodium 4.0kg, dispersant NNO6.0kg, film forming agent hydroxymethyl cellulose 3.0kg, warning coloration are basic rhodamine 2.0kg, thickening
Agent xanthans 0.2kg, antifreezing agent propane diols 4.0kg, preservative paraformaldehyde 0.3kg, organic silicon defoamer 0.2kg, separately use water
100kg is supplied, is well mixed and is crushed through ball milling, sand milling, disperses, can be made into 10% 100,000,000,000 living spores of ethiprole/g withered grass
The aqueous suspension agent 100kg of bacillus.
The effect test of embodiment 3
First, indoor sterilization insecticidal activity assay
1.1 sterilization(Rhizoctonia solani Kuhn)Determination of activity
(1)Test preparation medicament
Weigh bacillus subtilis starter medicine(1000000000000 spores/g)It is distributed in the 0.1% Tween 80 aqueous solution, is configured to one group
Bacillus subtilis single dose:If 6 concentration gradients, in terms of female medicine, concentration is respectively 4 μ g/mL(0.04 hundred million spores/gram)、8μg/
mL(0.08 hundred million spores/gram)、16μg/mL(0.16 hundred million spores/gram)、32μg/mL(0.32 hundred million spores/gram)、64μg/mL(0.64
Hundred million spores/gram)、128μg/mL(1.28 hundred million spores/gram).
Weigh bacillus subtilis starter medicine(1000000000000 spores/g)And ethiprole, it is distributed in the 0.1% Tween 80 aqueous solution,
Prepare 5 groups of ethiproles/bacillus subtilis intermixture:If 6 concentration gradients, in group the concentration of each dose of bacillus subtilis be 4,
8、16、32、64、128μg/mL(In terms of female medicine), the weight of the addition of ethiprole and bacillus subtilis starter medicine in each group medicament
Measure ratio respectively 5.1:0.4、5.05:0.45、5.0:0.5、4.95:0.55、4.9:0.6.
(2)Plating determination experiment
Each experimental pharmacy is proceeded as follows respectively:1mL test medicines are taken, are added to 45 DEG C or so, 49mL quantitatively trains
Support and poured into after being shaken up in base in culture dish, cooling is standby.By cultured pathogen, aseptically going out with diameter 5mm
Bacterium card punch steals bacteria cake from colony edge, and with inoculator by pure culture biscuits involvng inoculation in flat board center, mycelia is face-up, covers culture
Ware, in 25 DEG C of dark culturing 4d.Each medicament carries out 4 groups of parallel tests simultaneously, and adds culture medium with the 0.1% Tween 80 aqueous solution
As blank control.
(3)Investigation and statistical analysis
Investigation is measured at control group colony growth to culture dish edge.
Colony diameter is measured using crossing method, bacteriostasis rate is calculated, pharmacy test concentration and bacteriostasis rate is carried out respectively
Logarithm and niqueMin conversion, using log concentration value as independent variable, bacteriostasis rate niqueMin is dependent variable, carries out regression analysis, obtains
Virulence regression equation, EC50And EC90Coefficient correlation and coefficient of synergism, the data obtained are as shown in table 1.
Bactericide is killed according to the virulence size of these data evaluation bacillus subtilis Rhizoctonia solanis and ethiprole
The influence of bacterium effect.
1.2 desinsection(Grub)Determination of activity
(1)Test preparation medicament
Ethiprole is distributed in the 0.1% Tween 80 aqueous solution, prepares one group of ethiprole single dose:If 6 concentration gradients,
Respectively 5,10,20,40,80,160mg/L.
By ethiprole, bacillus subtilis starter medicine(1000000000000 spores/g)It is distributed in the 0.1% Tween 80 aqueous solution, prepares
5 groups of ethiproles and bacillus subtilis intermixture:If 6 concentration gradients, the concentration of wherein ethiprole is respectively 5,10,20,40,
80、160mg/L;The weight ratio of each group ethiprole and bacillus subtilis starter medicine is respectively:5.1:0.4、5.05:0.45、5.0:
0.5、4.95:0.55、4.9:0.6。
(2)Grub test method
Determined respectively to each test medicine above to the virulence of grub using pesticide-clay mixture method:Dry ground earth is crossed into 20 mesh testing sieves,
Then 800g is taken to be fitted into circular culture box(High 10cm, diameter 13cm), take 100ml test medicines to pour into wherein, Indoor Natural
Mixed uniformly after infiltration 2h, then the wheat seed that imbibition is showed money or valuables one carries unintentionally is mixed into wherein;Select 15 more consistent grubs of size and be put into box
It is interior, allow it voluntarily to bury(What can not voluntarily be buried will be changed), it is then placed in 26 DEG C of recovery room, knot is checked after 48h
Fruit.Each test medicine carries out 4 groups of parallel tests simultaneously, and is mixed into soil and is used as blank pair by the use of the 0.1% Tween 80 aqueous solution
According to.
(3)Investigation and statistical analysis
Substantially shunk with polypide during inspection result and be unable to scrambler and be considered as death.
The death rate is calculated, the death rate is corrected with Abbott formula, then using log concentration value as independent variable, school
The niqueMin of the positive death rate is dependent variable, asks the virulence regression equation, LC50, LC90, coefficient correlation of each single dose and complex preparation
And coefficient of synergism, the data obtained are shown in Table 2.
Virulence size by these data evaluation ethiproles to grub, analysis bacillus subtilis are big to ethiprole virulence
Small influence, determine the optimum proportioning of bacillus subtilis and ethiprole.
The experimental data of Tables 1 and 2 shows that ethiprole has certain facilitation to bacillus subtilis activity, withered
Careless bacillus also shows that certain synergistic effect to the insecticidal activity of ethiprole, the two reasonable compatibility, can simultaneously reach and kill
The effect of worm diseases prevention.
The ethiprole of table 1 and bacillus subtilis compound sterilizing synergy situation
The ethiprole of table 2 and bacillus subtilis compounding control of grubs synergy situation
2nd, field experiment
2010, Shandong Linqing experimental plot, the seed treatment to the present invention carry out wheat seed dressing and coating is tested, as a result
It is shown in Table 3;2011, Potou experimental plot, wheat seed dressing is carried out to the seed treatment of the present invention and coating is tested, result of the test
It is shown in Table 4.
The experimental data of table 3 and table 4 shows that seed treatment of the invention has the characteristics that:
1st, by crop seed coating or Dressing, to corn, the banded sclerotial blight of wheat, droop, full rot, root-rot
The multiple diseases such as disease, smut, there is preferable preventive effect to subterranean pest-insect.
2nd, the invasion that seed is dressed seed or after being coated seed can be made effectively to avoid pest and disease damage, field lasting period length have synergy
Effect.
3rd, said preparation effective ingredient ethiprole, bacillus subtilis, the two compatibility is safe, has production-increasing function, right
Environment compatibility is good.
4th, ethiprole of the invention and bacillus subtilis complex biological seed treatment are by dressing seed or being coated to seed
Comprehensive protection is provided, meets the developing direction of seed standardized production, there is larger society and economic value.The present invention's
Seed treatment not only facilitates the free of contamination Pesticidal products of production, while can also keep balance and the life of Agro-ecological System
Thing variation, is more beneficial for agricultural sustainable development.
Table Shandong in 32010 Linqing experimental plot prevents and treats Corn diseases and insect pests result of the test
Table Potou experimental field of wheat preventing and treating Wheat Diseases And Insect Pests result of the test in 42011