CN104706708A - Application of radix hedysari ethanol extract in preparation of medicine and health care products for preventing and curing liver fibrosis and effective component identification method thereof - Google Patents
Application of radix hedysari ethanol extract in preparation of medicine and health care products for preventing and curing liver fibrosis and effective component identification method thereof Download PDFInfo
- Publication number
- CN104706708A CN104706708A CN201510077488.2A CN201510077488A CN104706708A CN 104706708 A CN104706708 A CN 104706708A CN 201510077488 A CN201510077488 A CN 201510077488A CN 104706708 A CN104706708 A CN 104706708A
- Authority
- CN
- China
- Prior art keywords
- radix hedysari
- ethanol
- ethanol extraction
- radix
- hedysari
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Abstract
The invention provides an application of a radix hedysari ethanol extract in the preparation of medicine and health care products for preventing and curing liver fibrosis and an effective component identification method thereof. The preventive and therapeutic effect of the radix hedysari ethanol extract for the liver fibrosis of a mice caused by carbon tetrachloride is firstly discussed, ten batches of radix hedysari in different producing areas are selected for researching the anti-liver fibrosis pesticide effect of the radix hedysari, and the pesticide effect of a radix hedysari ethanol extractive part is further verified on the basis of a great number of samples; the main components of the radix hedysari ethanol extract are identified by applying a high performance liquid chromatography (HPLC) diode array detector (DAD), a fingerprint spectrum with good degree of separation and better peak shape is obtained through the optimization of a series of chromatographic conditions, five components such as adenosine, calycosin, ononin, fermlononetin and methylnissolin are identified, and a foundation is established for researching the components playing main roles in the radix hedysari ethanol extract.
Description
Technical field
The invention belongs to technical field of extraction of Chinese traditional medicine, be specifically related to Radix Hedysari ethanol extraction at the application prepared in prevention and therapy hepatic fibrosis medicines and health product and wherein effective ingredient authentication method.
Background technology
Radix Hedysari (Radix Hedysari), also referred to as " only son and heir ", is the dry root of leguminous plant Hedysarum polybotrys Hand.-Mazz. (Hedysarum polybotrys Hand.-Mazz.).Radix Hedysari slightly warm in nature, sweet in the mouth, return lung, spleen channel.There is tonifying Qi and lifting yang, strengthening superficial resistance to stop perspiration, inducing diuresis to remove edema, effect such as to nourish blood of promoting the production of body fluid.Its chemical composition mainly contains polysaccharide, flavone, saponin, trace element and aminoacid.
Hepatic fibrosis is a histopathology concept, it is the pathological change that multiple chronic hepatopathy has, hepatic fibrosis damages due to multiple the activation that liver factor causes hepatic stellate cell (HSC), extracellular matrix (ECM) too much growth and cause the short collagen degradations such as transforming growth factor β 1 (TGF-1) TNF and to reduce and caused by bulk deposition, the key link of its sustainable development is hepatocyte injury or the necrosis continuous stimulation to around nonparenchymal cell, thus cause ECM to synthesize in a large number and deposition, exceed its degradation speed, cause reconstruction and the destruction of hepatocyte structure thus, and develop into liver cirrhosis gradually.Hepatic fibrosis is a kind of active progress, the process of reconstructed tissue is formed in the reversible unbalance regulating liver-QI coagulation of YIN-cold in ZANG-organ of matrix metabolism, stimulates liver to cause inflammation formed by various impairment factor long-term chronics such as hepatitis virus, ethanol, medicine and poisonous substance, schistosomicide, metabolism and heredity, cholestasis, autoimmune liver disease.For the pathological characters that most of chronic hepatopathy is common, be also chronic hepatitis, the liver cirrhosis important intermediate link that further develops, worsen.Research shows, the 20%-40% in patients with liver fibrosis finally develops into liver cirrhosis and even hepatocarcinoma.The 1950's, first international hepatopathy academic authority Hans professor Popper proposes " who can stop hepatic fibrosis, and who just can cure most of hepatopathy ", therefore effectively blocks developing of hepatic fibrosis, significant to control liver cirrhosis hepatocarcinoma.
Single medicinal material and effective monomer treatment hepatic fibrosis also achieve certain progress, report that the more single medicinal material with effect of anti hepatic fibrosis mainly contains the Radix Astragali, Semen Persicae, Flos Carthami, Radix Notoginseng, Radix Salviae Miltiorrhizae, Radix Paeoniae Rubra, Radix Stephaniae Tetrandrae, Rhizoma Chuanxiong, Cordyceps, Radix Angelicae Sinensis, Radix Sophorae Flavescentis etc. more than tens and plants, to hepatic fibrosis, all there is good preventive and therapeutic effect, but major part do not further investigate play curative effect medicine contained by chemical composition.The present invention, by research 10 batches of Different sources Radix Hedysari, finds that its ethanol extraction has good result to hepatic fibrosis, identifies wherein 5 kinds of chemical compositions simultaneously, and the material playing drug effect for studying it is further laid a good foundation.
Summary of the invention
The invention provides Radix Hedysari ethanol extraction and preparing the application in prevention and therapy hepatic fibrosis medicines and health product, and the main chemical compositions wherein playing drug effect is identified.
First the present invention provides Radix Hedysari ethanol extraction preparing the application in prevention and therapy hepatic fibrosis medicines and health product.
Preferably, the effective ingredient of described Radix Hedysari ethanol extraction comprises adenosine, calycosin, ononin, formononetin and (-)-3-hydroxy-9-methoxypterocarpan.
Preferably, the preparation method of described Radix Hedysari ethanol extraction is: the 95%-50% alcohol reflux of use 2-10 times amount 3 times, each 1h, merge extractive liquid, lower floor's solution to without ethanol taste, by debris successively with after the petroleum ether equal with debris volume and extraction into ethyl acetate 5 times, is concentrated into dry by reclaim under reduced pressure, add 95%-50% ethanol, can be dissolved in wherein be Radix Hedysari ethanol extraction.
The present invention also provides the preparation method of Radix Hedysari ethanol extraction, step is as follows: the 95%-50% alcohol reflux of use 2-10 times amount 3 times, each 1h, merge extractive liquid, lower floor's solution to without ethanol taste, by debris successively with after the petroleum ether equal with debris volume and extraction into ethyl acetate 5 times, is concentrated into dry by reclaim under reduced pressure, add 95%-50% ethanol, can be dissolved in wherein be Radix Hedysari ethanol extraction.
3rd object of the present invention is to provide the authentication method of effective ingredient in Radix Hedysari ethanol extraction, Radix Hedysari ethanol extraction high performance liquid chromatography-diode array detector is identified, chromatographic column is Spursil C18 post, mobile phase is acetonitrile and water, gradient elution: 0-40min 40% acetonitrile, 60% water, 40-75min 60% acetonitrile, 40% water; Determined wavelength 260 nm; Flow velocity is 1.0 ml/min; Column temperature is 25 DEG C; Sample size is 20 μ l.
4th object of the present invention is to provide a kind of prevention and therapy hepatic fibrosis medicines, and the effective ingredient of described medicine is Radix Hedysari ethanol extraction.
Preferably, the dosage form of described medicine is solution, syrup, granule, capsule, powder, pill, tablet, freeze dried powder, patch, gel, membrane, drop pill, medicated wine, extractum or sustained-release preparation.
The preparation method of above-mentioned different dosage form medicine is identical with prior art.
Through test, Radix Hedysari ethanol extraction mice dosage is gastric infusion 12g crude drug/kg/ days, and adult human dose is oral administration 1g raw material/kg/ days.
5th object of the present invention is to provide a kind of prevention and therapy hepatic fibrosis health product, and the effective ingredient of described health product is Radix Hedysari ethanol extraction.
Preferably, the dosage form of described health product is solution, syrup, granule, capsule, powder, pill, tablet, patch, gel, membrane, extractum.
The application adopts the classical modeling mode of carbon tetrachloride, and it can accurately reflect hepatocyte function, metabolism and morphological change, and repeatability better; Choose simultaneously and have 5 characteristics Serum Indexes (AST, ALT, Tp, Alb, A/G) and liver heavy index thoroughly evaluating degree of hepatic fibrosis, experimental result is good; The present invention has inquired into the preventive and therapeutic effect of Radix Hedysari ethanol extraction to the hepatic fibrosis of carbon tetrachloride induced mice first, and select 10 batches of Different sources Radix Hedysari to study its anti-hepatic fibrosis drug effect, on the basis of great amount of samples, demonstrate the drug effect at Radix Hedysari ethanol extraction position further; High performance liquid chromatography (HPLC)-diode array detector (DAD) is used to identify wherein main component, by the optimization to a series of chromatographic condition, obtain separating degree good, peak shape is finger printing preferably, identify adenosine, calycosin, ononin, formononetin and (-)-3-hydroxy-9-methoxypterocarpan 5 compositions, for the composition playing Main Function in research Radix Hedysari ethanol extraction is laid a good foundation.The application is not by means of only research 10 batches of Different sources Radix Hedysari medical materials, demonstrate the drug effect of Radix Hedysari ethanol extraction anti-hepatic fibrosis further, have extensively studied the chemical composition in the Radix Hedysari playing this drug effect simultaneously, scheme clear and rational, result is good, is significant for the effective utilization and place of production expanding economy promoting Radix Hedysari herb resource.
Accompanying drawing explanation
Accompanying drawing is used to provide a further understanding of the present invention, and forms a part for description, together with embodiments of the present invention for explaining the present invention, is not construed as limiting the invention.In the accompanying drawings:
Fig. 1 is Radix Hedysari ethanol extraction position HPLC-DAD finger printing;
Fig. 2 is the HPLC-DAD finger printing of reference substance; Wherein, 1 is adenosine; 2 is calycosin; 3 is ononin; 4 is formononetin; 5 is (-)-3-hydroxy-9-methoxypterocarpan.
Detailed description of the invention
Following embodiment is convenient to understand the present invention better, but does not limit the present invention.Experimental technique in following embodiment, if no special instructions, is conventional method.Test material used in following embodiment, if no special instructions, is and purchases available from routine biochemistry reagent shop.
One, pharmacodynamic experiment
1. test material
1.1 animals: Kunming mouse, male, body weight 18-25g, Lanzhou University's animal experimental center provides, the quality certification number: SCXK(is sweet) 2013-0002.
1.2 main agents: acetonitrile (analytical pure, Merck); Wahaha Pure Water; Reference substance adenosine, calycosin, ononin, formononetin, (-)-3-hydroxy-9-methoxypterocarpan are (purchased from Chinese pharmaceutical biological product calibrating academy and Shanghai along vigorous biological engineering company limited, purity >98%) colchicines tablets (Xishuangbanna Pharmaceutical Co., Ltd, specification 0.5 mg/ grain); Carbon tetrachloride (CCl4, Tianjin Fu Yu Fine Chemical Co., Ltd); Oleum Arachidis hypogaeae semen (Luhua Group Co., Ltd., Shandong); Glutamic oxaloacetic transaminase, GOT test kit (ALT), glutamate pyruvate transaminase test kit (AST), total protein test kit (TP), albumin reagent box (ALB) (all building up biopharmaceutical company purchased from Nanjing); 10 batches of Gansu Province's Different sources Radix Hedysari medical materials, certainly adopt or buy, and numbering and source are in table 1.The dry root of Hedysarum polybotrys Hand.-Mazz. Hedysarum polybotrys Hand.-Mazz. is accredited as through pharmacognostical study institute of pharmaceutical college of Lanzhou University professor.
Table 1 Radix Hedysari crude drug source
1.3 key instruments: Waters Alliance 2695 high performance liquid chromatograph, comprises 2996 diode array detector, 717 automatic samplers, Millennium 32 chromatographic work station (Waters, US); Central South University's chromatographic fingerprints of Chinese materia medica area of computer aided similarity evaluation software (11.0); Rotary Evaporators (N-1100, Tokyo physics and chemistry apparatus [strain] individual proprietorship factory); Vortex vortex mixer (XK96-B, Xin Kang Medical Devices Co., Ltd. of Jiangyan City); 550 enzyme-linked immunosorbent assay instruments (Bio-Rad company of the U.S.); Ultrasonic cleaner (KQ3200BE numerical control, Ultrasound Instrument company limited of city of Kunshan); Sartorius BP211D electronic balance (error ± 0.00001, German Sai Duolisi); FA2104 electronic balance (error ± 0.0001, Shanghai City An Ting Electronic Instruments Plant).
1.4 statistical method: all test datas represent with mean ± standard deviation, carry out statistical disposition with SPSS 13.0 statistical package.Adopt one factor analysis of variance (ANOVA) organize between compare and the comparing between two of each class mean.
2. test procedure
2.1. the preparation of Radix Hedysari the different extracted parts: take Radix Hedysari 500g, with the 95%-50% alcohol reflux 3 times of 2-10 times amount, each 1h, merge extractive liquid, reclaim under reduced pressure, to without alcohol taste, is got debris 1/4 and is concentrated into dry, obtain Radix Hedysari total extract; Residue 3/4 moves to separatory funnel, respectively with the petroleum ether equal with debris volume and extraction into ethyl acetate 5 times, merges upper strata petroleum ether liquid and acetic acid ethyl fluid respectively, is concentrated into dry, is Petroleum ether extraction position and ethyl acetate extract part; Lower floor's solution is concentrated into dry, adds 95%-50% ethanol, can be dissolved in wherein be ethanol extraction position.According to this step, obtain Radix Hedysari total extract, petroleum ether part, ethyl acetate extract, ethanol position successively, according to the dosage of the paste-forming rate conversion mice of each extract part.
2.2. the effect of anti hepatic fibrosis of Radix Hedysari the different extracted parts: get male mice in kunming 84, after adaptability raises one week, be divided at random blank group, model group, positive controls and 4 Radix Hedysari extract part administration groups, often organize 12.Except blank group, all the other are respectively organized equal subcutaneous injection 40% carbon tetrachloride peanut oil solution and bring out hepatic fibrosis, and first dose of 0.1ml/10 g(doubles), 1 time/5d, continuous 35 days, can hepatic fibrosis be formed.Modeling simultaneously positive controls gavage gives colchicines tablets (0.6mg/kg/ days), and 4 administration group gavages give respective extract part (10g crude drug/kg/ days), and blank group and model group all give normal saline, and dosage is the same.After last administration, pluck eyeball and get blood, after standing centrifugal, isolate serum, illustrate by test kit and measure ALT, AST, Tp, Alb, A/G indices; Extract mouse liver simultaneously, weigh, calculate liver index.Concrete outcome is in table 2.
Table 2 Radix Hedysari the different extracted parts on carbon tetrachloride cause hepatic fibrosis in mice impact (
)
Note: compare with model group,
* * p<0.001,
* p<0.01,
* p<0.05.
As shown in Table 2, Radix Hedysari ethanol extraction position causes hepatic fibrosis in mice to carbon tetrachloride obvious inhibitory action, compared with model group each index all have significant difference (
p<0.001,
p<0.01).The research of effect of anti hepatic fibrosis is carried out at further selection 10 Different sources Radix Hedysari ethanol extraction position, to confirmatory experiment result on the basis of large sample.
2.3. the effect of anti hepatic fibrosis at Different sources Radix Hedysari ethanol extraction position:
Get 10 batches of Different sources Radix Hedysari, prepare ethanol extraction position by step under " 2.1 " item.Get male mice in kunming 156, be divided at random blank group, model group, positive controls and 10 batches of Radix Hedysari ethanol extraction regional administration groups, often organize 12.Each group of experimental technique and cycle are all with " 2.2 " item.Concrete outcome is in table 3.
Table 3 Different sources Radix Hedysari ethanol extraction position to the pharmacodynamics index of carbon tetrachloride induced mice hepatic fibrosis (
)
Note: compare with model group,
* * p<0.001,
* p<0.01,
* p<0.05.
As can be seen from Table 3,10 batches of Different sources Radix Hedysari ethanol extraction positions are compared with model group, comprehensive 6 indexs, majority parameters all has pole significant difference (P<0.001), hepatic fibrosis in mice modeling success is described, Radix Hedysari ethanol extraction position anti-hepatic fibrosis drug effect is remarkable.
Two, the HPLC finger printing at Radix Hedysari ethanol extraction position
1 chromatographic condition: chromatographic column is Spursil C18 post (250 × 4.6 mm, 5 μm); Mobile phase (gradient elution, A is acetonitrile, and B is water, 0-40 min:40%A; 40-75 min:60%A); Determined wavelength 260 nm; Flow velocity is 1.0 ml/min; Column temperature is 25 DEG C; Sample size is 20 μ l.
The preparation of 2 reference substance solution: precision takes reference substance adenosine, calycosin, ononin, formononetin, (-)-3-hydroxy-9-methoxypterocarpan 5.0mg, is placed in 5ml volumetric flask, adds the reference substance stock solution that methanol constant volume makes 1.0mg/ml respectively.Get each reference substance stock solution 0.9ml, be placed in same 5ml volumetric flask, add methanol constant volume, make the reference substance solution of 0.18mg/ml
The preparation of 3 need testing solutions: the dry product 0.5g getting Radix Hedysari medical material ethanol extraction position, accurately weighed, add dissolve with methanol and be transferred in 10ml measuring bottle, adding methanol dilution to scale, shake up, filtering, get filtrate, to obtain final product.
4 Radix Hedysari ethanol extraction position HPLC finger printing synergies
Respectively accurately draw adenosine, calycosin, ononin, formononetin, (-)-3-hydroxy-9-methoxypterocarpan reference substance and Radix Hedysari ethanol position need testing solution, measure according to above-mentioned chromatographic condition sample introduction, the results are shown in Figure 1, Fig. 2.By comparing with the retention time and uv absorption of reference substance, identifying 1 ~ No. 5 peak in the HPLC-DAD finger printing at Radix Hedysari ethanol extraction position and being respectively adenosine, calycosin, ononin, formononetin, (-)-3-hydroxy-9-methoxypterocarpan.
embodiment 1
The preparation method of Radix Hedysari ethanol extraction capsule of the present invention is:
After Radix Hedysari pulverizing medicinal materials, with 50% alcohol reflux 3 times of 6 times amount, each 1h, merge extractive liquid, reclaim under reduced pressure is extremely without ethanol taste, and debris moves to separatory funnel, respectively with the petroleum ether and extraction into ethyl acetate 5 times of debris equal volume after, lower floor's solution is concentrated into dry, adds 50% ethanol, dissolve, filter, remove insoluble matter, concentrated rear less than 60 DEG C dryings, add 80% ethanol granule, fill No. 1 capsule (loading amount is 0.1g-1.0g), obtain the capsule of Radix Hedysari ethanol extraction.
embodiment 2
The preparation method of Radix Hedysari ethanol extraction capsule of the present invention is:
After Radix Hedysari pulverizing medicinal materials, with 95% alcohol reflux 3 times of 2 times amount, each 1h, merge extractive liquid, reclaim under reduced pressure is extremely without ethanol taste, and debris moves to separatory funnel, respectively with the petroleum ether and extraction into ethyl acetate 5 times of debris equal volume after, lower floor's solution is concentrated into dry, adds 95% ethanol, dissolve, filter, remove insoluble matter, concentrated rear less than 60 DEG C dryings, add 60% ethanol granule, fill No. 1 capsule (loading amount is 0.1g-1.0g), obtain the capsule of Radix Hedysari ethanol extraction.
embodiment 3
The preparation method of Radix Hedysari ethanol extraction capsule of the present invention is:
After Radix Hedysari pulverizing medicinal materials, with 70% alcohol reflux 3 times of 5 times amount, each 1h, merge extractive liquid, reclaim under reduced pressure is extremely without ethanol taste, and debris moves to separatory funnel, respectively with the petroleum ether and extraction into ethyl acetate 5 times of debris equal volume after, lower floor's solution is concentrated into dry, adds 70% ethanol, dissolve, filter, remove insoluble matter, concentrated rear less than 60 DEG C dryings, add 75% ethanol granule, fill No. 1 capsule (loading amount is 0.1g-1.0g), obtain the capsule of Radix Hedysari ethanol extraction.
embodiment 4
The preparation method of Radix Hedysari ethanol extraction oral liquid of the present invention is:
After Radix Hedysari pulverizing medicinal materials, with 95% alcohol reflux 3 times of 2 times amount, each 1h, merge extractive liquid, reclaim under reduced pressure is extremely without ethanol taste, and debris moves to separatory funnel, respectively with the petroleum ether and extraction into ethyl acetate 5 times of debris equal volume after, lower floor's solution is concentrated into dry, adds 95% ethanol, can be dissolved in wherein be Radix Hedysari ethanol extract.Extracting solution is concentrated into the extractum without alcohol taste, in extractum, adds sweeting agent and correctives, then add water and obtain every 1ml liquid and be equivalent to 0.01-1g Radix Hedysari ethanol extraction beverage.
embodiment 5
The preparation method of Radix Hedysari ethanol extraction oral liquid of the present invention is:
After Radix Hedysari pulverizing medicinal materials, with 70% alcohol reflux 3 times of 6 times amount, each 1h, merge extractive liquid, reclaim under reduced pressure is extremely without ethanol taste, and debris moves to separatory funnel, respectively with the petroleum ether and extraction into ethyl acetate 5 times of debris equal volume after, lower floor's solution is concentrated into dry, adds 70% ethanol, can be dissolved in wherein be Radix Hedysari ethanol extract.Extracting solution is concentrated into the extractum without alcohol taste, in extractum, adds sweeting agent and correctives, then add water and obtain every 1ml liquid and be equivalent to 0.01-1g Radix Hedysari ethanol extraction beverage.
embodiment 6
The preparation method of Radix Hedysari ethanol extraction oral liquid of the present invention is:
After Radix Hedysari pulverizing medicinal materials, with 50% alcohol reflux 3 times of 10 times amount, each 1h, merge extractive liquid, reclaim under reduced pressure is extremely without ethanol taste, and debris moves to separatory funnel, respectively with the petroleum ether and extraction into ethyl acetate 5 times of debris equal volume after, lower floor's solution is concentrated into dry, adds 50% ethanol, can be dissolved in wherein be Radix Hedysari ethanol extract.Extracting solution is concentrated into the extractum without alcohol taste, in extractum, adds sweeting agent and correctives, then add water and obtain every 1ml liquid and be equivalent to 0.01-1g Radix Hedysari ethanol extraction beverage.
embodiment 7
The preparation method of Radix Hedysari ethanol extraction tablet of the present invention is:
The extracting method of Radix Hedysari is with embodiment 1 and 4, and after Radix Hedysari ethanol extract is concentrated into the extractum without alcohol taste, lyophilization, adds lubricant, disintegrating agent that pharmaceutical preparation is conventional, tabletting, film-making agent.
embodiment 8
The preparation method of Radix Hedysari ethanol extraction granule of the present invention is:
The extracting method of Radix Hedysari is with embodiment 1 and 4, and after Radix Hedysari ethanol extract is concentrated into the extractum without alcohol taste, lyophilization, adds wetting agent, diluent that pharmaceutical preparation is conventional, granule processed.
embodiment 9
The preparation method of Radix Hedysari ethanol extraction capsule of the present invention is:
The extracting method of Radix Hedysari is with embodiment 1 and 4, and after Radix Hedysari ethanol extract is concentrated into the extractum without alcohol taste, lyophilization, add wetting agent, diluent that pharmaceutical preparation is conventional, granule processed, fills No. 1 capsule (loading amount is 0.1g-1.0g).
Last it is noted that the foregoing is only the preferred embodiments of the present invention, be not limited to the present invention, although with reference to previous embodiment to invention has been detailed description, for a person skilled in the art, it still can be modified to the technical scheme described in foregoing embodiments, or carries out equivalent replacement to wherein portion of techniques feature.Within the spirit and principles in the present invention all, any amendment done, equivalent replacement, improvement etc., all should be included within protection scope of the present invention.
Claims (9)
1. the application in prevention and therapy hepatic fibrosis medicines and health product prepared by Radix Hedysari ethanol extraction.
2. application according to claim 1, is characterized in that: the effective ingredient of described Radix Hedysari ethanol extraction comprises adenosine, calycosin, ononin, formononetin and (-)-3-hydroxy-9-methoxypterocarpan.
3. application according to claim 1, it is characterized in that: the preparation method of described Radix Hedysari ethanol extraction is: the 95%-50% alcohol reflux of use 2-10 times amount 3 times, each 1h, merge extractive liquid, lower floor's solution to without ethanol taste, by debris successively with after the petroleum ether equal with debris volume and extraction into ethyl acetate 5 times, is concentrated into dry by reclaim under reduced pressure, add 95%-50% ethanol, can be dissolved in wherein be Radix Hedysari ethanol extraction.
4. the preparation method of Radix Hedysari ethanol extraction, it is characterized in that: step is as follows: the 95%-50% alcohol reflux of use 2-10 times amount 3 times, each 1h, merge extractive liquid, lower floor's solution to without ethanol taste, by debris successively with after the petroleum ether equal with debris volume and extraction into ethyl acetate 5 times, is concentrated into dry by reclaim under reduced pressure, add 95%-50% ethanol, can be dissolved in wherein be Radix Hedysari ethanol extraction.
5. the authentication method of effective ingredient in Radix Hedysari ethanol extraction, it is characterized in that: Radix Hedysari ethanol extraction high performance liquid chromatography-diode array detector is carried out isolation identification, chromatographic column is Spursil C18 post, mobile phase is acetonitrile and water, gradient elution: 0-40min 40% acetonitrile, 60% water, 40-75min 60% acetonitrile, 40% water; Determined wavelength 260 nm; Flow velocity is 1.0 ml/min; Column temperature is 25 DEG C; Sample size is 20 μ l.
6. a prevention and therapy hepatic fibrosis medicines, is characterized in that: the effective ingredient of described medicine is Radix Hedysari ethanol extraction.
7. medicine according to claim 6, is characterized in that: the dosage form of described medicine is solution, syrup, granule, capsule, powder, pill, tablet, freeze dried powder, patch, gel, membrane, drop pill, medicated wine, extractum or sustained-release preparation.
8. prevention and therapy hepatic fibrosis health product, is characterized in that: the effective ingredient of described health product is Radix Hedysari ethanol extraction.
9. health product according to claim 8, is characterized in that: the dosage form of described health product is solution, syrup, granule, capsule, powder, pill, tablet, patch, gel, membrane, extractum.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510077488.2A CN104706708B (en) | 2015-02-13 | 2015-02-13 | Red stilbene ethanol extract prevents and treats application and wherein effective component identification method in hepatic fibrosis medicines in preparation |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510077488.2A CN104706708B (en) | 2015-02-13 | 2015-02-13 | Red stilbene ethanol extract prevents and treats application and wherein effective component identification method in hepatic fibrosis medicines in preparation |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN104706708A true CN104706708A (en) | 2015-06-17 |
| CN104706708B CN104706708B (en) | 2018-11-20 |
Family
ID=53406753
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201510077488.2A Active CN104706708B (en) | 2015-02-13 | 2015-02-13 | Red stilbene ethanol extract prevents and treats application and wherein effective component identification method in hepatic fibrosis medicines in preparation |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN104706708B (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114437100A (en) * | 2021-12-29 | 2022-05-06 | 郑州师范学院 | Method for extracting santalin from sea sword beans and application of santalin |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20060110473A1 (en) * | 2003-04-29 | 2006-05-25 | Li Min Pharmaceutical Factory Of Livzon Pharmaceutical | Composition containing radix codonopsis pilosulae and radix astragali and hedysari, method of producing it and use thereof |
| CN101272799A (en) * | 2005-08-12 | 2008-09-24 | 凡诺华（英国）有限公司 | Plant-based medicament for the treatment of liver disease |
| CN101690742A (en) * | 2009-10-10 | 2010-04-07 | 周广生 | Application of radix hedysari in preparing medicament for treating animal infectious diseases |
| CN102885878A (en) * | 2012-09-29 | 2013-01-23 | 甘肃中医学院 | Application of effective parts of astragalus mongholicus and hedysarum polybotrys |
| CN103450296A (en) * | 2013-08-29 | 2013-12-18 | 山西大学 | Method for rapidly preparing forsythin from forsythia suspense leaves |
-
2015
- 2015-02-13 CN CN201510077488.2A patent/CN104706708B/en active Active
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20060110473A1 (en) * | 2003-04-29 | 2006-05-25 | Li Min Pharmaceutical Factory Of Livzon Pharmaceutical | Composition containing radix codonopsis pilosulae and radix astragali and hedysari, method of producing it and use thereof |
| CN101272799A (en) * | 2005-08-12 | 2008-09-24 | 凡诺华（英国）有限公司 | Plant-based medicament for the treatment of liver disease |
| CN101690742A (en) * | 2009-10-10 | 2010-04-07 | 周广生 | Application of radix hedysari in preparing medicament for treating animal infectious diseases |
| CN102885878A (en) * | 2012-09-29 | 2013-01-23 | 甘肃中医学院 | Application of effective parts of astragalus mongholicus and hedysarum polybotrys |
| CN103450296A (en) * | 2013-08-29 | 2013-12-18 | 山西大学 | Method for rapidly preparing forsythin from forsythia suspense leaves |
Non-Patent Citations (6)
| Title |
|---|
| 包海鹰: "《毒蘑菇化学成分与药理活性的研究》", 31 December 2006 * |
| 杨智等: "中药红芪的化学成分研究", 《中国中药杂志》 * |
| 红芪贡酒: "保健酒的未来很精彩", 《HTTP://BLOG.SINA.COM.CN/S/BLOG_1332CA2420102V0TZ.HTML》 * |
| 苏韫等: "红芪黄酮对肺纤维化模型大鼠基质金属蛋白酶-2及其抑制剂-1蛋白表达的影响", 《中国中医药信息杂志》 * |
| 覃红萍等: "不同种、不同产地黄芪中黄酮类HPLC指纹图谱研究", 《中国药师》 * |
| 韩涛等: "红芪多糖抗肝纤维化的药效作用", 《中国老年学杂志》 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114437100A (en) * | 2021-12-29 | 2022-05-06 | 郑州师范学院 | Method for extracting santalin from sea sword beans and application of santalin |
Also Published As
| Publication number | Publication date |
|---|---|
| CN104706708B (en) | 2018-11-20 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Yan et al. | Discovery of anti-2019-nCoV agents from Chinese patent drugs via docking screening | |
| CN1925864B (en) | Plant-based medicament for the treatment of hepatitis c | |
| CN100384467C (en) | Traditional Chinese medicine composition for treating hepatitis and fatty liver | |
| CN103751529A (en) | Traditional Chinese medicine composition for treating female postmenopausal osteoporosis and preparation method thereof | |
| CN103877244A (en) | Medicinal composition for treating headache and preparation method thereof | |
| CN101721488B (en) | Pharmaceutical composition for treating liver diseases and prepration method thereof | |
| CN106924406B (en) | A pharmaceutical composition for adjuvant treatment of AIDS and its preparation method | |
| CN104490972A (en) | Pharmaceutical composition for treating and preventing depression and preparation method of pharmaceutical composition | |
| CN104288245B (en) | Anti-aging and constitutional pharmaceutical composition and preparation method thereof and detection method | |
| CN103550476A (en) | Medicine composition for treating postmenopausal osteoporosis, and preparation method thereof | |
| CN103784761A (en) | Drug for treatment of vertigo and preparation method thereof | |
| CN1923263B (en) | Traditional Chinese medicine composition, its preparing method and quality controlling means | |
| CN111437338A (en) | Application of traditional Chinese medicine composition in treating skin diseases | |
| CN104225206A (en) | Sugar-free Changchun longevity granules and preparation method thereof | |
| CN104042895A (en) | Traditional Chinese medicine composition for treating systemic lupus erythematosus and use thereof | |
| CN1927324A (en) | Preparation method of traditional medicine preparation for treating women's menoxenia | |
| CN104706708A (en) | Application of radix hedysari ethanol extract in preparation of medicine and health care products for preventing and curing liver fibrosis and effective component identification method thereof | |
| CN111375035A (en) | Preparation and application of enteric-coated preparation for treating cervical spondylosis | |
| CN111562324B (en) | Method for detecting contents of multiple index components of breast-eliminating and stasis-removing capsule | |
| CN109288907B (en) | Yandan capsule, preparation method, detection method and new application thereof | |
| CN105232627A (en) | Solidago extract and preparation method and medical application thereof | |
| CN105456591A (en) | Manufacturing method of cedar seed preparation for heart nourishing | |
| CN104524171A (en) | Application of traditional Chinese medicine preparation in preparing medicine for treating hepatitis | |
| CN100350939C (en) | Chinese medicine for treating nonbacterial prostatitis and its prepn | |
| CN104645080A (en) | Traditional Chinese medicine preparation for treating dizziness and preparation method of traditional Chinese medicine preparation |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |