CN104673495A - Method for extracting volatile oil for preventing and treating gynecologic inflammation from self-heal - Google Patents
Method for extracting volatile oil for preventing and treating gynecologic inflammation from self-heal Download PDFInfo
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Abstract
The invention relates to a method for extracting volatile oil for preventing and treating gynecologic inflammation from self-heal, which comprises the following steps: 1) weighing self-heal raceme powder, adding 8-10 times by mass of methanol or ethanol solution as an extracting solution, extracting by an ultrasonic process, filtering, collecting the filtrate, repeatedly extracting 2-4 times, merging the filtrates, filtering the merged filtrate, and concentrating to obtain an extractum with the water content of 15-20%; and 2) mixing 100-200-mesh silica gel with the extractum, stirring uniformly, adding the uniform mixture into a silica gel column, flushing with petroleum ether, collecting the flushing fluid, and volatilizing petroleum ether to obtain the self-heal volatile oil. The method is high in extraction yield and easy to implement; and the extracted volatile oil has favorable drug effect and is suitable for wide popularization.
Description
Technical field
The invention belongs to grease and extract field, be specifically related to the extraction of volatile oil in Spica Prunellae.
Background technology
Spica Prunellae is the dry fruit ear of labiate Spica Prunellae (Prunella vulgaris L.), in China, Spica Prunellae integrated distribution is dirty area in Basin of Huaihe River and the Changjiang river, the abundantest with the wild resource standing stock in Jiangsu, Anhui, Zhejiang and Henan; In addition Korea S, Japan, also there is distribution Ukraine and Kashmir etc.Spica Prunellae main component has triterpene and saponin(e, phenolic acid, sterol and glycosides thereof, flavones, tonka bean camphor, organic acid, volatile oil and carbohydrate etc.
Spica Prunellae has long applicating history in medicinal, and primary efficacy has clearing liver-fire, improving eyesight, mass dissipating and swelling eliminating; For conjunctival congestion with pain and swelling of the eye, order pearl nyctalgia, has a headache dizzy, scrofula, goiter and tumor, acute mastitis, newborn addiction, distending pain of the breast (Pharmacopoeia of the People's Republic of China version in 2010).Shennong's Herbal is recorded, and " Spica Prunellae bitter is pungent, cold, main hot scrofula, mouse fistula, and head is created, broken disease, loose goitre, and knot gas, pin swells, and arthritis with fixed pain caused by dampness, makes light of one's life by commiting suicide.One sunset sentence, a Nedong.Raw Chuan Gu.”。Except do medicinal except, Spica Prunellae also uses as food.Compendium of Material Medica is recorded " time Spica Prunellae tender seedling fell, bitter taste is removed in leaching, and it is edible that oily salt is mixed." " food book on Chinese herbal medicine " record " Spica Prunellae, taste is worked hard, and cold, nontoxic, tender seedling changed, and bitter taste is removed in leaching, and it is mixed by oily salt, to eat as the marshland full of water weeds, splendid U.S..”
Although Spica Prunellae has long history as drug use, but the mainly Spica Prunellae aqueous extract of report and the pharmacological action of alcohol extract, and have no the extraction of its volatile oil part of report and treating gynaecopathia, as the application of the aspects such as chronic pelvic inflammatory disease, cervicitis, mazoitis.
Summary of the invention
The object of this invention is to provide the extracting method of volatile oil in a kind of Spica Prunellae, and provide the application of Spica Prunellae volatile oil in control gynecological inflammation medicine, its extraction step is as follows:
1) Spica Prunellae fruit ear powder is taken, be incorporated as its quality 8-10 methyl alcohol doubly or ethanolic soln as extracting solution, ultrasonic method is adopted to extract, filter, collect filtrate, identical Ultrasonic Conditions repeats extraction 2 ~ 4 times, merging filtrate, and the filtrate after being combined is filtered and the medicinal extract of simmer down to water content 15% ~ 20% again;
2) mix with described medicinal extract with 100-200 order silica gel, and stir, the mixture stirred loaded silicagel column and rinses with sherwood oil, collecting washing fluid, volatilize sherwood oil, obtain Spica Prunellae volatile oil.
In the present invention, described Spica Prunellae fringe powder size is 20-40 order.
In the present invention, described extracting solution is methyl alcohol or ethanolic soln, adopts these two kinds of extracting solutions to recycle, pollution-free, has good economic benefit and environmental benefit.
In the present invention, the concentration of described methyl alcohol, ethanol extract is respectively 60%-100%, 50%-90%.Above concentration selected respectively by methyl alcohol and ethanol two kinds of solvents, and penetrance is good, and extraction efficiency is high, and the extract water-soluble impurity obtained with this solvent is few.
In the present invention, the power of described supersound extraction is 900-1000W, and extraction time is 40-60min.Under this power, the mass-transfer efficiency of extract solutes is high, can more rapidly and efficiently extract; Ultrasonic time can ensure higher extraction efficiency within the scope of this, and the impact of time expand on extraction yield is little.
In the present invention, the mass ratio of described silica gel and medicinal extract is 1:1 ~ 1:2.Can ensure higher post effect with this ratio, and its loading volume is less.
In the present invention, the flow velocity of described sherwood oil is 40-70ml/min, rinses to eluent color close to colourless.
The extracting method of volatile oil in Spica Prunellae of the present invention, preferably includes following steps:
1) taking granularity is 40 order Spica Prunellae fruit ear powder, be incorporated as its quality 10 times of 60% methanol solution, mix, adopt supersound extraction, ultrasonic power is 900W, continuous extraction 60min, filter, collect filtrate, repeat extraction under identical Ultrasonic Conditions 3 times, merging filtrate, the filtrate after being combined again is filtered and is condensed into the medicinal extract of water content 18%;
2) 100-200 object silica gel is joined described medicinal extract with the mass ratio of 1:1.5 to stir, the mixture stirred is loaded silicagel column and rinses with sherwood oil, the flow velocity of sherwood oil is 50mL/min, flushing is colourless to the color of sherwood oil, obtain washing fluid, volatilize sherwood oil, obtain Spica Prunellae volatile oil.
Another object of the present invention is that protection uses method of the present invention to extract the Spica Prunellae volatile oil obtained.
The present invention extracts the Spica Prunellae volatile oil obtained, and can apply the medicine of gynaecopathia such as preparation control gynecological inflammation chronic pelvic inflammatory disease and cervicitis medicine etc.
Method of the present invention, is extracted grease by ultrasonic method crush method on the one hand, have employed penetration power good, be easy to the methyl alcohol that reclaims or ethanol is solvent; Make use of the effect such as ultrasonic cavitation effect and mechanical vibration effect fully, target component can be made to enter solvent more fast, shorten extraction time, thus improve extraction efficiency.
On the other hand rinse purifying by silicagel column with sherwood oil and obtain Spica Prunellae volatile oil, this method is simple, and solvent load is few and be easy to recycle.Therefore, present method is that a kind of technique is simple, the method for the extraction Spica Prunellae volatile oil that extraction efficiency is high.
Accompanying drawing explanation
Fig. 1 Spica Prunellae volatile oil on Carrageenan causes the impact of rat paw edema
Embodiment
Following examples for illustration of the present invention, but are not used for limiting the scope of the invention.
Embodiment 1
The present embodiment relates to the extracting method of volatile oil in a kind of Spica Prunellae, and concrete steps are as follows:
1,40 order Spica Prunellae fruit ear powder 500g are taken with electronic balance, be incorporated as 100% methanol solution of its quality 8 times, mix, adopt supersound extraction, ultrasonic power is 1000W, continuous extraction 40min, filter, collect filtrate, extract 3 times continuously at the same terms, merging filtrate, the filtrate after being combined again is filtered and is condensed into the medicinal extract of water content about 15%;
2,100-200 object silica gel is joined in described medicinal extract with the mass ratio of 1:1 stir, the mixture stirred is loaded silicagel column and rinses with sherwood oil, the flow velocity of sherwood oil is 40mL/min, rinse to the color of sherwood oil close to colourless, obtain washing fluid, volatilize sherwood oil, Spica Prunellae volatile oil yield is 3.32 ‰.
Gas chromatograph-mass spectrometer (GC-MS) is utilized to analyze the chemical composition of the Spica Prunellae volatile oil of embodiment 1.Result shows, separation obtains 53 kinds of compounds, main chemical compositions has: trans-9-octadecenoic acid (56.92%), Palmitic acid (15.27%), n-Octadecanoic acid (12.49%), cis-11-Eicosenoic acid (2.17%), trans-Squalene (1.26%), Methyl 18-methylnonadecanoate (1.16%), Tetrahydropyranyl ether of citronellol (1.11%), n-Tetratetracontane (0.83%), Tetracosanoic acid (0.81%), 15-Tetracosenoic acid (0.79%) etc.
Embodiment 2
The present embodiment relates to the extracting method of volatile oil in a kind of Spica Prunellae, and concrete steps are as follows:
1,40 order Spica Prunellae fruit ear powder 500g are taken with electronic balance, be incorporated as its quality 10 times of 60% methanol solution, mix, adopt supersound extraction, ultrasonic power is 900W, continuous extraction 60min, filter, collect filtrate, extract 3 times continuously under the same conditions, merging filtrate, the filtrate after being combined again is filtered and is condensed into the medicinal extract of water content about 18%;
2,100-200 object silica gel is joined described medicinal extract with the mass ratio of 1:1.5 to stir, the mixture stirred is loaded silicagel column and rinses with sherwood oil, the flow velocity of sherwood oil is 50mL/min, flushing is colourless to the color of sherwood oil, obtain washing fluid, volatilize sherwood oil, the yield of Spica Prunellae volatile oil is 4.06 ‰.
Embodiment 3
The present embodiment relates to the extracting method of volatile oil in a kind of Spica Prunellae, and concrete steps are as follows:
1,20 order Spica Prunellae fruit ear powder 500g are taken with electronic balance, be incorporated as its quality 9 times of 90% ethanolic soln, adopt supersound extraction, ultrasonic power is 950W, extracts 50min continuously, filters, collect filtrate, extract 3 times continuously under the same conditions, merging filtrate, the filtrate after being combined again is filtered and is condensed into the medicinal extract that water content is about 20%.
2,100-200 object silica gel is joined described medicinal extract with the mass ratio of 1:2 to stir, the mixture stirred is loaded silicagel column and rinses with sherwood oil, the flow velocity of sherwood oil is 70mL/min, flushing is colourless to the color of sherwood oil, obtain washing fluid, volatilize sherwood oil, Spica Prunellae volatile oil yield is 2.97 ‰.
Embodiment 4
The present embodiment relates to the extracting method of volatile oil in a kind of Spica Prunellae, and concrete steps are as follows:
1,30 order Spica Prunellae fruit ear powder 500g are taken with electronic balance, be incorporated as its quality 10 times of 50% ethanolic soln, adopt supersound extraction, ultrasonic power is 1000W, extracts 45min continuously, filters, collect filtrate, extract 3 times continuously under the same conditions, merging filtrate, the filtrate after being combined again is filtered and is condensed into the medicinal extract that water content is about 20%;
2,100-200 object silica gel is joined described medicinal extract with the mass ratio of 1:1 to stir, the mixture stirred is loaded silicagel column and rinses with sherwood oil, the flow velocity of sherwood oil is 70mL/min, flushing is colourless to the color of sherwood oil, collect washing fluid, volatilize sherwood oil, the yield of Spica Prunellae volatile oil is 3.51 ‰.
Comparative example 1
Compare with embodiment 2, difference is, be incorporated as Spica Prunellae fruit ear powder quality 15 times, concentration is the methanol solution of 30%, the interest rate of Spica Prunellae volatile oil is 1.52 ‰.
Comparative example 2
Compare with embodiment 2, difference is, adopt 300 ~ 400 object silica gel, the mass ratio of silica gel and medicinal extract is 1:3, and the interest rate of Spica Prunellae volatile oil is 1.72 ‰.
Experimental example 1 Spica Prunellae volatile oil is to the effect of mice auricle swelling
Experimental subjects: laboratory animal is SPF level Female ICR mice, body weight 18-22g, credit number: SCXK (Hunan) 2011-0003, and Si Laike Jing Da laboratory animal company limited provides by Hunan; Laboratory room temperature 20-22 DEG C, relative humidity 40%-60%, ventilator is ventilated, and lamp 12h/ day, raises in cages, 10, every cage.
Experimental technique: gastric infusion is carried out to mouse; Dosage is 4g/kg (crude drug amount); Administration volume is 1ml/100g.
In this experiment, experimental group is that embodiment 1 extracts the Spica Prunellae volatile oil obtained, blank is 5 ‰ carboxymethylcellulose sodium solutions (5 ‰ CMC-Na), positive drug contrast is dexamethasone tablet (1.82mg/kg), Zhejiang Province XianJu Pharmacy stock Co., Ltd, lot number 140204, administration volume is 10ml/kg.
Get the female mice 40 of body weight 18-22g, be divided into 4 groups at random.Every day gastric infusion 1 time, administration capacity is 10ml/kg, continuous 6 days, within 7th day, last administration is after 1 hour, at auris dextra tow sides each 20 μ L distilled water of normal group mouse, respectively group mouse right ear tow sides each 20 μ L caused by dimethylbenzene xylene are scorching for all the other, after 1h, after mouse cervical dislocation is put to death, horse back eye scissors cuts mouse ears, round auricle is laid at the same position cutting ears respectively with 9mm punch tool, with analytical balance (AL 104-electronic balance, Mettler-Toledo Instrument (Shanghai) Co., Ltd.) weigh, record each group of mouse left and right ear weight.
Experimental result: this experimental data is all with Excel and SPSS software processes.Experimental data is in table 1:
The impact (n=10, x ± s) of table 1 Spica Prunellae volatile oil p-Xylol induced mice auricle edema
Note: compare with model group: * P < 0.05, * * P < 0.01
Experimental result shows, compared with model group, administration group auricle swelling degree obviously reduces (P < 0.05 or P < 0.01), illustrate that Spica Prunellae volatile oil has obvious restraining effect to mice auricle swelling, and its resistance rate is close with Dexamethasone group, prove that Spica Prunellae volatile oil has good restraining effect to mice auricle swelling.
The effect of experimental example 2 pairs of rat toes swelling
Experimental subjects: laboratory animal is SPF level female sd inbred rats, body weight 180-220g, credit number: SCXK (Hunan) 2011-0003, and Si Laike Jing Da laboratory animal company limited provides by Hunan; Laboratory room temperature 20-22 DEG C, relative humidity 40%-60%, ventilator is ventilated, and lamp 12h/ day, raises in cages, 5, every cage.
Experimental technique: get the female rats 40 between body weight 180-220g, weigh, be divided into 4 groups at random.Every day gastric infusion 1 time, administration capacity is 10ml/kg, continuous 6 days, and administration measures the right back sufficient sole of the foot thickness of each mouse with digital display thickness gauge on the 7th day, as every mouse administration front foot sole of the foot thickness.30min after last administration, at the right back sufficient sole of the foot position subcutaneous injection 0.9% sodium chloride solution 0.1ml/ of rats in normal control group only, only causes inflammation at other group rat right hind leg foot sole of the foot position subcutaneous injection 1% carrageenin suspensions 0.1ml/ respectively.After causing inflammation, 30min, 60min, 120min, 180min, 240min, 360min digital display thickness gauge (Taizhou plain Ai Ce Instrument Ltd., precision 0.01mm) measures right back sufficient sole of the foot thickness, record data respectively.
The mode of administration described above is, the volatile oil that Example 1 extracts, and dosage is 3.2g/kg (crude drug amount); Route of administration is gastric infusion; Administration volume is 10ml/kg.
This experiment blank is 5 ‰ carboxymethylcellulose sodium solutions (5 ‰ CMC-Na), and positive drug contrast is dexamethasone tablet (1.2mg/kg), and Zhejiang Province XianJu Pharmacy stock Co., Ltd, lot number 140204, administration volume is 10ml/kg.
Experimental result: this experimental data is all with Excel and SPSS software processes.Experimental result is in table 2, and its figure that becomes is as Fig. 1.
Table 2 Spica Prunellae volatile oil on Carrageenan cause rat paw edema impact (n=10,
)
Note: compare with model group: * P < 0.05, * * P < 0.01
Experimental result shows, and model group, compared with control group, all has obvious tumefaction at individual time point, and modeling success is described; The each administration group of Spica Prunellae volatile oil starts to play a role after 60min, later each time point is compared with model group, administration group toes swelling obviously reduces (P < 0.05 or P < 0.01), and the rat paw edema that Spica Prunellae volatile oil can obviously suppress carrageenin to cause is described.
The effect of experimental example 3 pairs of rat chronic pelvic inflammatory disease
Experimental subjects: laboratory animal is SPF level female sd inbred rats, body weight 180-220g, credit number: SCXK (Hunan) 2011-0003, and Si Laike Jing Da laboratory animal company limited provides by Hunan; Laboratory room temperature 20-22 DEG C, relative humidity 40%-60%, ventilator is ventilated, and lamp 12h/ day, raises in cages, 5, every cage.
Experimental technique: get rat with after the vetanarcol intraperitoneal injection of anesthesia of 30mg/kg, rat is fixed on surgical console, in rat uterus angle place injection 0.2ml mixed bacteria liquid (streptococcus aureus: intestinal bacteria: beta hemolytic streptococcus=1:1:2), sterilization is layering skin suture also.
Get modeling success rat after postoperative about 15 days and be divided into 5 groups at random, often organize 10, successive administration about 30 days, after last administration 12h, put to death rat, expose uterus, observe uterine state, take out uterus and weigh, calculate organ index; Be placed in 4% paraformaldehyde again to fix, routine paraffin wax embedded section, HE dyes, and light Microscopic observation respectively organizes rat pathological change.
The process of about 30 days of described successive administration, the volatile oil that experimental example uses embodiment 1 to extract, dosage is 3.2g/kg (crude drug amount); Route of administration is gastric infusion; Administration volume is 10ml/kg.
This experiment blank is equal-volume 5 ‰ CMC-Na, and positive drug contrast is dexamethasone tablet (1.2mg/kg), and Zhejiang Province XianJu Pharmacy stock Co., Ltd, lot number 140204, administration volume is 10ml/kg.
Experimental result: this experimental data is all with Excel and SPSS software processes.
Morphological observation: normal group is substantially identical with sham operated rats (distinguish with model group: only open abdomen, do not inject mixed bacteria liquid, get rid of the impact of operation) situation: uterus size, form, color are all normal, with surrounding tissue without adhesion, without congestion and edema; Model group (blank group): uterus color burn, has congestion and edema, and uterine cavity has hydrops, with surrounding tissue adhesion, difficult separation when drawing materials; Positive drug group: uterus size, form are normal, accidental slight hyperemia, uterine cavity have hydrops, with surrounding tissue substantially without adhesion; Volatile oil group uterus color is deepened a little, has slight congested or fluid in uterine situation, less with surrounding tissue adhesion.
Pathological observation: normal group is substantially identical with sham operated rats situation: uterine cavity wall construction is complete, clear, uterine cavity is without expansion or adhesion, and mucosal epithelial cells form is normal, and gland structure is clear, glandular epithelium marshalling, rare inflammatory cell infiltration; Model group: the damage of uterine cavity wall construction is serious, muscle layer is unclear with stratum mucosum structure, mucosal epithelial cells sex change, necrosis, body of gland necrosis, adenomatosis, inner membrance congestion and edema, massive inflammatory cells infiltrated; Positive drug group: uterine cavity wall construction is substantially complete, uterine cavity is without expansion or adhesion, and body of gland is normal, the visible inflammatory cell infiltration of part; Volatile oil administration group situation is substantially identical: chamber wall construction is substantially complete, and mucosal epithelial cells has mild hyperplasia, and part has cavity, and body of gland major part is normal, and inner membrance congestion and edema is not obvious, and inflammatory cell infiltration is less.
Organ index experimental data is in table 3:
Table 3 Spica Prunellae volatile oil part on the impact of rat chronic pelvic inflammatory disease organ index (n=10,
)
Note: compare with model group: * P < 0.05, * * P < 0.01
From table 3, the uterus index of this experiment volatile oil administration group is starkly lower than model group, illustrates that injection mixed bacteria liquid can cause chronic pelvic inflammatory disease rat model organ index to raise.
Volatile oil can reduce organ index, also has obvious improvement to pathology damage, P < 0.05 compared with model group, illustrates that Spica Prunellae volatile oil has certain therapeutic action to rat chronic pelvic inflammatory disease caused by mixed bacteria liquid.
The effect of experimental example 4 pairs of rat cervicitises
Experimental subjects: laboratory animal is SPF level female sd inbred rats, body weight 180-220g, credit number: SCXK (Hunan) 2011-0003, and Si Laike Jing Da laboratory animal company limited provides by Hunan; Laboratory room temperature 20-22 DEG C, relative humidity 40%-60%, ventilator is ventilated, and lamp 12h/ day, raises in cages, 5, every cage.
Experimental technique: after rat etherization, in every mouse intravaginal injection 0.4ml25% Hydroxybenzene mucilage (point two some injections, often some 0.2ml), every injection in 3 days 1 time, continuous 4 times; Normal group often only injects equal-volume solvent.
Get 40 modeling success rats, be divided into 4 groups, be respectively normal group, model group, positive drug group, volatile oil administration group; The 4th day after modeling first starts administration, successive administration 10 days, after last administration 12h, puts to death rat, takes out vagina and uterine neck part and weigh, calculate organ index; Be placed in 4% paraformaldehyde again to fix, routine paraffin wax embedded section, HE dyes, and light Microscopic observation respectively organizes rat pathological change.
The administration group of embodiment 1, dosage is 3.2g/kg (crude drug amount); Route of administration is gastric infusion; Administration volume is 10ml/kg.
This experiment blank is equal-volume 5 ‰ CMC-Na, and positive drug contrast is dexamethasone tablet (1.2mg/kg), and Zhejiang Province XianJu Pharmacy stock Co., Ltd, lot number 140204, administration volume is 10ml/kg.
Experimental result: this experimental data is all with Excel and SPSS software processes.
Pathological observation: normal group cervical tissue structural integrity, accidental a small amount of inflammatory cell infiltration, telasubmucosa is normal; Model group epithelium is seriously rotten to the corn, massive inflammatory cells infiltrated, and submucosa telangiectasis is congested, fibroblast proliferation; Positive drug group, volatile oil administration group all have and to a certain degree take a turn for the better compared with model group, and inflammatory cell infiltration obviously reduces, and epithelial cell is substantially complete, rotten to the corn not serious.
Organ index experimental data is in table 4:
Table 4 Spica Prunellae volatile oil part on the impact of rat cervicitis organ index (n=10,
)
Note: compare with model group: * P < 0.05, * * P < 0.01
This experiment shows that Spica Prunellae volatile oil can reduce Rats Organs and Tissues index, and pathology damage is clearly better, and illustrates that caused by Spica Prunellae volatile oil Pyrogentisinic Acid rubber cement, rat cervicitis has certain curative effect.
Comprehensive above pharmacodynamic experiment, first two kinds of classical mice auricle swellings and rat paw edema model views Spica Prunellae volatile oil is adopted to have certain result for the treatment of to rat inflammation model, observe its therapeutic action to rat chronic pelvic inflammatory disease and cervicitis more further, main detection its on the impact of the aspect such as organ index, histopathology, result shows that Spica Prunellae volatile oil causes rat chronic pelvic inflammatory disease and Hydroxybenzene mucilage to mixed bacteria liquid and causes rat cervicitis and have certain therapeutic action.
Although above the present invention is described in detail with a general description of the specific embodiments, on basis of the present invention, can make some modifications or improvements it, this will be apparent to those skilled in the art.Therefore, these modifications or improvements without departing from theon the basis of the spirit of the present invention, all belong to the scope of protection of present invention.
Claims (9)
1. prevent and treat an extracting method for volatile oil in the Spica Prunellae of gynecological inflammation, it is characterized in that, comprise the steps:
1) Spica Prunellae fruit ear powder is taken, be incorporated as its quality 8-10 methyl alcohol doubly or ethanolic soln as extracting solution, ultrasonic method is adopted to extract, filter, collect filtrate, repeat extraction 2 ~ 4 times, merging filtrate, the filtrate after being combined is filtered and the medicinal extract of simmer down to water content 15% ~ 20% again;
2) mix with described medicinal extract with 100-200 order silica gel, stir, the mixture stirred loaded silicagel column and rinses with sherwood oil, collecting washing fluid, volatilize sherwood oil, obtain Spica Prunellae volatile oil.
2. extracting method according to claim 1, is characterized in that, the granularity of described Spica Prunellae fringe powder is 20 ~ 40 orders.
3. extracting method according to claim 1, is characterized in that, the concentration of described methanol extract liquid is 60%-100%, and the concentration of described ethanol extract is 50%-90%.
4. extracting method according to claim 1, is characterized in that, the power of described supersound extraction is 900-1000W, and extraction time is 40-60min.
5. extracting method according to claim 1, is characterized in that, the mass ratio of described silica gel and medicinal extract is 1:1 ~ 1:2.
6. extracting method according to claim 1, is characterized in that, the flow velocity of described sherwood oil is 40-70mL/min, and the color of rinsing to sherwood oil is colourless.
7. the extracting method according to any one of claim 1 ~ 6, is characterized in that, comprises the steps:
1) taking granularity is 40 order Spica Prunellae fruit ear powder, be incorporated as its quality 10 times of 60% methanol solution, mix, adopt supersound extraction, ultrasonic power is 900W, continuous extraction 60min, filter, collect filtrate, repeat extraction under the same terms 3 times, merging filtrate, the filtrate after being combined again is filtered and is condensed into the medicinal extract that water content is 18%;
2) 100-200 object silica gel is joined described medicinal extract with the mass ratio of 1:1.5 to stir, the mixture stirred is loaded silicagel column and rinses with sherwood oil, the flow velocity of sherwood oil is 50mL/min, flushing is colourless to the color of sherwood oil, obtain washing fluid, volatilize sherwood oil, obtain Spica Prunellae volatile oil.
8. the arbitrary extracting method of claim 1-7 extracts the Spica Prunellae volatile oil obtained.
9. the application of Spica Prunellae volatile oil described in claim 8 in preparation control gynecological inflammation chronic pelvic inflammatory disease and cervicitis medicine.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107496502A (en) * | 2017-08-11 | 2017-12-22 | 湖南中医药大学 | Selfheal volatile oil is preparing the application in treating fibroid medicine |
| CN108567958A (en) * | 2018-07-25 | 2018-09-25 | 杭州光启医疗科技发展有限公司 | A kind of pharmaceutical composition and preparation method thereof for treating gynaecological imflammation |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1977849A (en) * | 2005-12-02 | 2007-06-13 | 天津市世雄科技发展有限公司 | Kibe ointment with bactericidal-bacteriostasis function |
| CN101347502A (en) * | 2007-07-20 | 2009-01-21 | 天津天士力制药股份有限公司 | Effective component of selfheal and preparation and use thereof |
| CN103157048A (en) * | 2011-12-15 | 2013-06-19 | 深圳太太药业有限公司 | Traditional Chinese medicine composition for treating chronic pelvic inflammation and preparation method thereof |
-
2015
- 2015-02-10 CN CN201510069333.4A patent/CN104673495B/en not_active Expired - Fee Related
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1977849A (en) * | 2005-12-02 | 2007-06-13 | 天津市世雄科技发展有限公司 | Kibe ointment with bactericidal-bacteriostasis function |
| CN101347502A (en) * | 2007-07-20 | 2009-01-21 | 天津天士力制药股份有限公司 | Effective component of selfheal and preparation and use thereof |
| CN103157048A (en) * | 2011-12-15 | 2013-06-19 | 深圳太太药业有限公司 | Traditional Chinese medicine composition for treating chronic pelvic inflammation and preparation method thereof |
Non-Patent Citations (1)
| Title |
|---|
| 余茜等: "夏枯草果穗的化学成分", 《中国实验方剂学杂志》 * |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107496502A (en) * | 2017-08-11 | 2017-12-22 | 湖南中医药大学 | Selfheal volatile oil is preparing the application in treating fibroid medicine |
| CN107496502B (en) * | 2017-08-11 | 2020-07-07 | 湖南中医药大学 | Application of prunella vulgaris volatile oil in preparation of medicine for treating hysteromyoma |
| CN108567958A (en) * | 2018-07-25 | 2018-09-25 | 杭州光启医疗科技发展有限公司 | A kind of pharmaceutical composition and preparation method thereof for treating gynaecological imflammation |
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