CN100408085C - Snow lotus preparation for treating prostate disease - Google Patents
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- CN100408085C CN100408085C CNB2006100842950A CN200610084295A CN100408085C CN 100408085 C CN100408085 C CN 100408085C CN B2006100842950 A CNB2006100842950 A CN B2006100842950A CN 200610084295 A CN200610084295 A CN 200610084295A CN 100408085 C CN100408085 C CN 100408085C
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Abstract
The present invention relates to a snow lotus preparation for treating prostate diseases, which is made of raw material medicine of snow lotuses, gromwells, zedoaries, safflowers, breed tubers, frankincense and myrrh and auxiliary material of glyceride of mixed fatty acid and lauryl sodium sulfate, and is medicine for external use for treating prostate and prostate hyperplasia (hypertrophy). Proved by a tryout result, the present invention has the advantages of antibiosis, inflammation relief and analgesia, enhances the immunity of machine bodies, notably suppresses the prostate hyperplasia, enhances the generation of blood serum and hemolysin, enhances the content of blood serum bacteriolysis, clears various surplus free radicals in the bodies, and enhances the activity of antioxidant enzyme. Simultaneously, envelope wall breaking factors with high concentration are contained to make the snow lotus preparation for treating prostate diseases have a direct bactericidal function to golden brown staphylococci, colibacillus, protei, epidermal staphylococci, pyocyanine, white candida, treponemas, gonorrhoeae diplococcus, trichomonads, etc. The snow lotus preparation for treating prostate diseases has the advantages of clearing away heat, detoxicating, activating blood circulation, dissipating blood stasis, inducing diuresis and reducing edema, has effective speed to incurable chronic prostatitis and the prostate hyperplasia (the hypertrophy). Simultaneously, treatment of principal and subordinate symptoms is carried out, with original efficacy.
Description
Technical field
The present invention relates to snow lotus preparation for treating prostate disease of the acute and chronic prostatitis of a kind of control, prostatic hyperplasia genitourinary system such as (hypertrophys) and preparation method thereof.
Background technology
Prostatitis and prostatic hyperplasia are male's commonly encountered diseases, and this disease is strict to influence patient's physical and mental health and quality of life, has caused the world of medicine's extensive concern.Chronic prostatitis can be divided into bacillary and non-bacterial two big classes, its cause of disease, pathology complexity, and the special anatomical features of prostate in addition, (having blood-prostate barrier), its conventional antibiotic therapy method is DeGrain often.Present radio-frequency (RF) thermotherapeutic, Therapeutic Method such as melt in the per urethra prostata tissue and obtained certain therapeutic advance, but range of application is limited, though operative treatment has advance, but operating technology there is higher requirement, complication such as urinary incontinence, hangover of gland and TURP syndrome may occur if operation is not held concurrently.
Drug treatment, owing to doctor trained in Western medicine also makes further progress to the prostatic hyperplasia Study of Etiology, so the effect of treatment with western is satisfied not enough.Treatment at present mainly concentrates on the e receptor blocking; 5 alpha-reductases suppresses; hormone therapy several aspects such as (anti-androsterone treatment are main); obtained certain curative effect; but the patient often suffers from dizziness, orthostatic hypotension simultaneously; sexual disorder appears in part patient, phenomenons such as endocrine dysfunction, and drug price costliness.
Summary of the invention
The object of the invention is that series preparation is the training medication of treatment prostatitis and prostatic hyperplasia (hypertrophy) before the Herba Saussureae Involueratae of development.Said preparation is by crude drug Herba Saussureae Involueratae, Radix Arnebiae (Radix Lithospermi), Rhizoma Curcumae, Flos Carthami, rhizoma sparganic, Olibanum and Myrrha, and adjuvant is that mixed fatty glycerides and 1% sodium lauryl sulphate are made; Result of the test shows, before Lenin's oral liquid share snow lotus preparation for treating prostate disease and have antibiotic, antiinflammatory, analgesia, enhancing human body immunity power, significantly suppress prostatic hyperplasia, improve the serum hemolysin generation and improve serum bacteriolyze content, remove various unnecessary free radicals in the body, improve the activity of antioxidase, include the high concentration peplos breaking cellular wall factor simultaneously, make this medicine focus of going directly, staphylococcus aureus, escherichia coli, Bacillus proteus, staphylococcus epidermidis, bacillus pyocyaneus, Candida albicans, treponema pallidum, gonorrhea diplococcus, infusorian etc. are had direct bactericidal action.Clinical research shows, this product heat-clearing and toxic substances removing, blood circulation promoting and blood stasis dispelling, inducing diuresis to remove edema have onset rapid to cureless chronic prostatitis and prostatic hyperplasia (hypertrophy), the treating both the principal and the secondary aspects of a disease at the same time unique effects.Through a large amount of retrieval, do not see frequent micturition due to the treatment prostatitis that above a few flavor medical material compatibility makes, urgent micturition, dysurea, waist is aching and limp, the dripping turbid preparation that waits disease, and wherein chemistry, pharmacology and clinical practice document of each flavor are more.
Snow lotus preparation for treating prostate disease of the present invention; said preparation is by crude drug Herba Saussureae Involueratae, Radix Arnebiae (Radix Lithospermi), Rhizoma Curcumae, Flos Carthami, rhizoma sparganic, Olibanum and Myrrha; adjuvant is that mixed fatty glycerides and 1% sodium lauryl sulphate are made, and the content of per 1000 or piece each component is: Herba Saussureae Involueratae 500-600g, Flos Carthami 350-400g, Radix Arnebiae (Radix Lithospermi) 1100-1150g, Rhizoma Curcumae 620-690g, rhizoma sparganic 620-690g, Olibanum 150-200g, Myrrha 150-200g, mixed fatty glycerides 1.40-1.80kg and sodium lauryl sulphate 15-20g.
The preparation method of snow lotus preparation for treating prostate disease follows these steps to carry out:
A, will Olibanum, Myrrha is ground into fine powder after concocting, and crosses 100 mesh sieves, mixing is standby;
B, Rhizoma Curcumae are ground into coarse powder, add 8 times of water gagings, extract volatile oil according to a conventional method 6 hours, and volatile oil device is in addition collected, and is standby;
C, medicinal residues and Herba Saussureae Involueratae, Flos Carthami, rhizoma sparganic add 10 times of amount 80% alcohol reflux 3 times, each 1.5 hours, merging filtrate filters, and it is 1.15～1.20 clear paste that filtrate decompression is concentrated into relative density, vacuum drying gets dry extract, pulverize, mixes mistake 100 mesh sieves with fine powder after above-mentioned Olibanum, Myrrha are pulverized, as mixed powder, standby;
D, Radix Arnebiae (Radix Lithospermi) add 12 times of amount 95% ethanol and carry out percolation with the speed of per minute 7ml, collect percolate, and be to thick paste, standby at decompression recycling ethanol below 60 ℃;
E, take by weighing S by the amount of 2.3 times of mixed powders
36Mixed fatty glycerides and 1% sodium lauryl sulphate; be heated to molten condition; add mixed powder and thick paste; fully stir evenly, the volatile oil that adds during to 45 ℃ to be cooled continues to be stirred to evenly; inject the bolt mould; take out cooling back, make 1000 or piece, or make paste according to a conventional method and get final product.
Snow lotus preparation for treating prostate disease prevents, treats the purposes of the external used medicine of acute and chronic prostatitis, prostatic hyperplasia, prostate hyperplasia as preparation.
Using method of the present invention: preceding the rising with morning of sleeping filled in medicine anus 4-6 centimeters after an action of the bowels, and every day 2 times, each one piece or one, one month is a course of treatment.
Snow lotus preparation for treating prostate disease of the present invention is to choose pure natural Uigurs medicines such as Herba Saussureae Involueratae according to the theory that the Uygur medicine pharmacy shows unique characteristics, cause vitality, mentality, the insufficient sign of natural agent at " vara rice I the Mai Zi of Germany and Italy ", prescription emphasizes to strengthen human life power, mentality, natural agent, effects such as maturation and the interior unusual body fluid of removing body, develop last my bolt of Herba Saussureae Involueratae, Herba Saussureae Involueratae last I loose, Herba Saussureae Involueratae prostatitis pad, and share with preceding Lenin's oral liquid, for treatment prostatitis and prostatic hyperplasia (hypertrophy) provide new tool and new method.
Snow lotus preparation for treating prostate disease of the present invention is a foundation with uighur medicine scientific principle opinion, adopt the refining pure natural Uygur medicine type external suppository that forms of modern pharmaceutical technology, include the high concentration peplos breaking cellular wall factor, make this medicine focus of going directly, to cureless chronic prostatitis and prostatic hyperplasia (hypertrophy) have onset rapidly, the unique effects for the treatment of both the principal and the secondary aspects of a disease at the same time.This medicine is not found toxic and side effects, and nonirritant does not have irritated reaction, steady quality, and the medicine source is abundant, and is easy to use etc.
The pharmacological properties of snow lotus preparation for treating prostate disease of the present invention is:
Herba Saussureae Involueratae: this product is the dry low top of feverfew Herba Saussureae Involueratae Saussure ' a involucrateKar.et.Kin..The property of medicine is xeothermic, bitter in the mouth, suffering, and is poisonous.Have to give birth to do and give birth to heat, dispeiling pathogenic wind and removing dampness, bone and muscle strengthening, anti-inflammatory analgetic, vulcanize kidney, hypersexuality, promoting blood circulation to restore menstrual flow, tire is urged in the temperature palace, and effects such as cold relieving leukorrhagia stopping are used for raw property or lymphatic temperament disease, catch cold rheumatic arthritis, arthralgia as the waist kidney, kidney is cold empty, hyposexuality, through water not down, under the Placenta Hominis difficulty, lower abdomen cold, diseases such as leucorrhoea grow in quantity.
Flos Carthami: be the dried floral of feverfew Flos Carthami Carthamus tinctorius L..Property of medicine secondary is xeothermic.Have to give birth to do and give birth to heat, promoting blood circulation to restore menstrual flow, ripe unusual lymphatic temperament, nourishing and fit keeping function, enriching blood blazes, and dampness is removed tinea, the antiinflammatory detumescent, the diuresis replenishing essence, relieving cough and asthma, the warm blood tonifying YANG, convergence removes effects such as rash.Be used for raw property or lymphatic temperament disease, as menoxenia, dysuria, oligospermia sexual impotence, cough with copious phlegm, asthma out of breath, vitiligo, white macula disease, eczema, oral ulcer etc.
Radix Arnebiae (Radix Lithospermi): be the dry root of comfrey lithospermum euchromum Royle Arnebia euchroma (Royle) Johnst., Radix Arnebiae (Radix Lithospermi) Lithospermum erythrorhizonSieb.etZucc. or arnebia guttata Bunge Arnebiaguttata Bunge.The property of medicine is done cold.Have to give birth to do to give birth to and tremble with fear promoting blood circulation to restore menstrual flow, effects such as eliminating inflammation and expelling toxin.Be used for humid disease such as hepatitis, measles without adequate eruption, carbuncle, diseases such as scabies.
Rhizoma Curcumae: be the dry rhizome of zingiberaceous plant Rhizoma Curcumae Curcuma phaeocaulis Val., Guangxi zedoary Curcuma kwangsiensis S.G.Lee et C.F.Liang or RADIX CURCUMAE Curcumawenyujin Y.H.Chen et C.Ling.Property of medicine secondary is xeothermic, acrid in the mouth.Have to give birth to do and give birth to heat, open retardance, the warming the meridian detumescence, the gas pain relieving of loosing, promoting blood circulation to remove obstruction in the collateral, warm waist tonifying YANG, the supplementing the brain heart tonifying, invigorating the stomach and promoting digestion, warming the kidney to invigorate YANG, diuresis is stimulated the menstrual flow, sending down the abnormal ascending QI emesis, effects such as strong flesh teeth consolidating.Be used for raw property or lymphatic temperament disease, as the swollen and pain of various inflammation that retardance causes, cough, asthma, lower abdominal distention pain, arthralgia, the stomachache poor appetite, the lumbago sexual impotence, infrequent menstruation is not smooth, nausea and vomiting, odontoseisis, chronic gingivitis.
SANLING: for the dry stem of rhizoma scirpi plant rhizoma scirpi Sparganium stoloniferum Buch.-Ham. fast.The property of medicine is xeothermic.Has blood-activating and qi-promoting, effects such as removing obstruction for relieving pain.Be used for the blood stasis amenorrhea, diseases such as food stagnation distending pain.
Olibanum: for Burseraceae Boswellia carterii Boswellia carterii Birdw. and congener B.bhaw-dijiana Birdw. bark ooze out resin.The dried secondary heat of property of medicine one-level, acrid in the mouth, hardship.Have to give birth to do and give birth to heat, the antiinflammatory detumescent is removed the tinea wound healing, convergence granulation promoting, antiasthmatic-antitussive, stomach warming emesis, invigorating the stomach and promoting digestion, effects such as controlling nocturnal emission with astringent drugs reducing urination.Be used for raw property or lymphatic temperament brain diseases, as moist brain void, paronychia, mastitis, encelitis is swollen, and seminal fluid is not solid, the frequent micturition incontinence, tinea capitis, burn, nebula, diseases such as various skin infection are given birth in the canthus.
Myrrha: be olive subject plant Herba Violae tree Commiphora myrrha Engl., breathe out the dry resin of Herba Violae tree Commiphora molmol Engl..Property of medicine secondary is xeothermic, bitter in the mouth, suffering.Have the heat of give birth to do giving birth to, the damp eliminating pain relieving of tremble with fear, softening the hard mass removes tumor, and remove tinea and more create, the cold relieving cough-relieving, drying dampness to eliminate phlegm, erosion prevention and muscle growing stimulates the menstrual flow, effects such as diuresis detoxifcation.Be used for raw property or lymphatic temperament disease, as raw property arthralgia, sciatica, oral cavity pain, have sore throat cold cough, moist abundant expectoration, bronchiectasis, eczema abscess, menoxenia, dysuria etc.
The present invention amplifies production according to this production technology, obtains the suppository of three batches of amplifications, its outward appearance, melts the key property that becomes suppositorys such as time limit and all meets " the pertinent regulations under 2000 editions suppository items of Chinese pharmacopoeia.
Snow lotus preparation for treating prostate disease of the present invention was observed 6 months under long-term reserved sample observing 12 months, acceleration environment, significant change does not all take place in sample appearance, character, discriminating, hardness, content uniformity, disintegration, microbial limit and content, shows that this quality of the pharmaceutical preparations is stable, package materials selection is appropriate.
The pharmacological toxicology research of snow lotus preparation for treating prostate disease of the present invention:
Main pharmacodynamics research:
Experimental animal: Kunming mouse, Wistar rat, available from the big medical experiment animal center of Xinjiang medical courses in general, the certificate of competency: No. 16003, the moving word of Xinjiang doctor.
(1) the Herba Saussureae Involueratae prostate suppository causes bullate influence to mice foot sole of the foot carrageenin
Method and result: get 80 of Kunming kind white mice, body weight 20 ± 2g, be male, divide 8 groups at random, administration fasting in preceding 4 hours, every day 1 time, rectally is 7 days continuously, and filled in anus 4 hours with spongy embolus, injected the subcutaneous inflammation that causes of the right back sufficient sole of the foot of mice with 1% carrageenin 0.05ml in 1 hour after the last administration, cause scorching back 1,2,3,4,5, measured sufficient sole of the foot thickness with outside micrometer in 6 hours, so that the difference of the sufficient sole of the foot is organized a mean t check for the swelling degree before and after scorching, and the results are shown in Table 1 according to " (blank group swelling degree-administration group swelling degree)/blank group swelling degree ":
Table 1 Herba Saussureae Involueratae prostate suppository causes bullate influence (X ± SD n=10) to mice foot sole of the foot carrageenin
Administration group and blank group group compare,
* *P＜0.01,
*P＜0.05,
*P＞0.05.
As seen from Table 1, Herba Saussureae Involueratae prostate suppository various dose and positive control drug Flos Chrysanthemi Indici suppository rectally, on Carrageenan induced mice pedal swelling all has significant inhibitory effect.Show that Herba Saussureae Involueratae prostate suppository on Carrageenan induced mice acute exudative inflammation has significant antiinflammatory inhibitory action.
(2) the Herba Saussureae Involueratae prostate suppository is to the bullate influence of mice auricular concha caused by dimethylbenzene xylene
Method and result: get 50 of mices, body weight 20 ± 2g is male.Divide 5 groups and rectally at random, administration fasting in preceding 4 hours, every day 1 time, successive administration 7 days, and filled in anus 4 hours with spongy embolus, after the last administration 30 minutes, every group of mouse right ear two sides smeared dimethylbenzene 0.1ml and caused inflammation, caused scorching 2 hours and take off neck to put to death animal, get same position, left and right sides auricle with the 8mm card punch, weighing, is the swelling degree with two auricle weight differences, organizes a mean t check.And, the results are shown in Table 2 according to " (the average swelling degree of the blank group-average swelling degree of administration group)/average swelling degree of blank group group " calculating inflammation suppression ratio.
Table 2 Herba Saussureae Involueratae prostate suppository is to the bullate influence of mice auricular concha caused by dimethylbenzene xylene (X ± SD)
Administration group and blank group compare,
* *P＜0.01.
As seen from Table 2, the Herba Saussureae Involueratae prostate suppository is little, in, behind big three kinds of dosage and the positive control medicine suppository of wild chrysanthemum flower agent rectally, the two shell inflammation swelling of xylol induced mice all have significant inhibitory effect.Show that the Herba Saussureae Involueratae prostate suppository has stronger inhibitory action to acute exudative inflammation.
(3) the Herba Saussureae Involueratae prostate suppository is to the swollen influence that forms of rat granuloma
Method and result: get 40 of wistar rats, body weight 200 ± 20g.The Herba Saussureae Involueratae prostate suppository group that is divided into blank group, Flos Chrysanthemi Indici suppository positive controls and 3 various dose according to the body weight equilibrium at random.Each rat is under the ether light anaesthesia, and left and right sides groin is respectively cut an osculum, expands subcutaneous tissue with vascular forceps, and every side is imbedded 20mg sterilization cotton balls, then sews up.Postoperative began rectally the same day, every day 1 time, and filled in anus 4 hours, continuous 7 days with spongy embolus.Pulling cervical vertebra on the 8th day puts to death, cut open and get cotton balls, pick most fat, it is heavy to deduct cotton balls after weighing, be the clean weight in wet base of granuloma, with t check between the clean wet weight mean of each group granuloma work group, and, the results are shown in Table 3 according to " (the clean weight in wet base of the blank group granuloma-clean weight in wet base of administration group granuloma)/clean weight in wet base of blank group granuloma " calculating suppression ratio.
Table 3 Herba Saussureae Involueratae prostate suppository is to the swollen influence that forms of rat granuloma (X ± SD)
Administration blank group compares with group:
* *P＜0.01.
As seen from Table 3, little with the Herba Saussureae Involueratae prostate suppository, in, big three kinds of dosage or the continuous rectally of positive control drug Flos Chrysanthemi Indici suppository 7 days, bullate formation all has significant inhibitory effect to rat granuloma.Show that the Herba Saussureae Involueratae prostate suppository also has the obvious suppression effect to proliferative inflammation.
(4) the Herba Saussureae Involueratae prostate suppository is to the influence of rat dry yeast artificial fever
Method and result: get 40 of Wistar male rats, 200 ± 20g, what be divided into blank group and 3 dosage at random is tried thing group and positive controls, administration fasting in preceding 4 hours, simultaneously dips in normal saline insertion anus with bare headed Glass rod earlier before the administration, rectally behind the abundant defecation of stimulation in rats, and filled in anus 4 hours with spongy embolus, every day 1 time, continuous 7 days.Survey basal body temperature before the last administration earlier, each Mus of 1h subcutaneous injection 20g/dl yeast suspension 1ml/100g in the back immediately after the administration, after this 0.5,1,2,4,6,8,10,12h respectively surveys animal heat once, surveyed anus temperature and basic anus using warming therapy difference with different time, index for the body temperature variation, each group is carried out statistical procedures, the results are shown in Table 4.
Table 4 Herba Saussureae Involueratae prostate suppository is to the influence (X ± SD, n=8) of rat dry yeast artificial fever
Administration group and blank group compare,
* *P＜0.01,
*P＜0.05,
*P＞0.05.
As seen from Table 4, behind the subcutaneous injection yeast of the rat back suspension, body temperature has rising by a relatively large margin, behind Herba Saussureae Involueratae prostate suppository and the positive control drug rectally, more all can make rat fever body temperature that remarkable reduction effect is arranged with the blank group, show that the Herba Saussureae Involueratae prostate suppository has refrigeration function when heavy dose of.
(5) the Herba Saussureae Involueratae prostate suppository is to the analgesic activity of mice
1, mice hot plate method
Method and result: choose the Healthy female mice, earlier each Mus is placed respectively on 55 ± 0.5 ℃ of hot plates, with mice kicking, to lick metapedes, jump be the pain reaction index, selects 50 of the qualified mices that pain reaction was no more than for 30 seconds incubation period, body weight 20 ± 2g, what be divided into blank group and 3 dosage at random is tried thing group and positive controls, rectally, administration fasting in preceding 4 hours, every day 1 time, and filled in anus 4 hours, successive administration 7 days with spongy embolus.After the last administration, begin to measure 15 minutes, 30 minutes, 60 minutes pain thresholding, and calculate threshold of pain raising percentage rate according to " average pain threshold * 100% before (the preceding average pain threshold of average pain threshold one medication after the medication)/medication ".The results are shown in Table 5.
Table 5 Herba Saussureae Involueratae prostate suppository is to the analgesic activity (mice hot plate method) of mice (X ± SD)
Administration group and blank group compare,
* *P＜0.01,
*P＜0.05.
As seen from Table 5, the Herba Saussureae Involueratae prostate suppository is little, in, behind big three kinds of dosage and the positive control drug rectally, all have significant analgesic activity.
2, mouse writhing method
Method and result: get 50 of healthy male mices, body weight 20 ± 2g is tried thing group and positive controls according to what the body weight equilibrium divided physiology saline control group and 3 dosage at random, rectally, and every day 1 time, and filled in anus 4 hours, successive administration 7 days with spongy embolus.After the last administration 1 hour, the equal ip0.6% acetic acid of each Mus 0.2ml, and write down and turn round writhing response in body incubation period and the 15min (abdominal part indent, stretch hind leg, buttocks is raised) number of times behind each Mus ip acetic acid, with the index of writhing response number of times as the analgesic activity comparison, organize a mean t check, and calculate the analgesia percentage rate according to " (the blank group is on average turned round body number of times-medication group and on average turned round the body number of times)/blank group is on average turned round the body number of times ".The results are shown in Table 6.
Table 6 Herba Saussureae Involueratae prostate suppository is to the analgesic activity (mouse writhing method) of mice (X ± SD)
Administration group and blank group compare,
* *P＜0.01.
As seen from Table 6, the Herba Saussureae Involueratae prostate suppository is little, in, behind big three kinds of dosage and the positive control drug rectally, (stomachache reaction) has significant inhibitory effect to the caused writhing response of mouse peritoneal injection acetum, shows that the Herba Saussureae Involueratae prostate suppository has significant analgesic activity.
(6) influence of Herba Saussureae Involueratae prostate suppository Dichlorodiphenyl Acetate induced mice peritoneum inflammatory exudation
Method and result: get 50 of male mices, body weight 20 ± 2g, grouping and medication are the same, continuous 7 days.1h after the last administration, the equal iv of each Mus give 0.5% azovan blue normal saline solution 5ml/kg, and ip0.6% acetic acid 10ml/kg immediately.20min puts to death mice behind ip acetic acid, use the normal saline flushing abdominal cavity, collect whole flushing liquors to 10ml, get supernatant after centrifugal and measure its OD value,, reflect the degree of peritoneum inflammatory exudation indirectly with the concentration of azovan blue in the OD value representation flushing liquor in ultraviolet spectrophotometer 578nm wavelength place, compare with the blank group, t check between the work group, and, the results are shown in Table 7 according to " (the average OD value of the blank group-average OD value of administration group)/average OD value of blank group " calculating suppression ratio:
The influence of table 7 Herba Saussureae Involueratae prostate suppository Dichlorodiphenyl Acetate induced mice peritoneum inflammatory exudation (X ± SD)
Administration group and blank group compare,
* *P＜0.01,
*P＜0.05,
*P＞0.05.
As seen from Table 7, the Herba Saussureae Involueratae prostate suppository is little, in, behind big three kinds of dosage and the positive control drug rectally, the Herba Saussureae Involueratae prostate suppository has the obvious suppression effect to the mouse peritoneum inflammatory exudation when heavy dose of, positive control drug Flos Chrysanthemi Indici suppository also has significant inhibitory effect.
(7) the Herba Saussureae Involueratae prostate suppository is to the therapeutical effect of chronic prostatitis
1. the Herba Saussureae Involueratae prostate suppository is to the prostatitic therapeutical effect of rat chronic due to the escherichia coli
Experimental technique: choose 60 of Wistar male rats, body weight 240-280g is divided into dosage group (V) in normal group (I), model group (II), Flos Chrysanthemi Indici suppository group (III), Herba Saussureae Involueratae prostate suppository small dose group (IV), the Herba Saussureae Involueratae prostate suppository, the heavy dose of group of Herba Saussureae Involueratae prostate suppository (VI) according to the body weight equilibrium.
Except that the normal control group, all the other 5 groups of rat administration 1 Zhou Houjun are with etherization, after waiting to anaesthetize, wipe out lower abdomen by hair, the abdomen median line is cut down stomach wall open in the aseptic condition lower edge, mentions prostate gently with tweezers, injects the good escherichia coli (1 * 10 of dilution in advance respectively in both sides
7) each 0.2ml, send attached internal organs, sew up abdominal wall muscle skin, put back to mouse cage.After the modeling, it is relatively poor that each organizes the rat mental status, and movable the minimizing is off one's feed, but all progressively recover in postoperative beginning in the 3rd day.Observed seven days continuously, each organizes none death of rat, and wound healing is good, and the mental status can be good, and hair color is moist, and appetite, two is just no abnormal.
Medication: each is organized rat and injects escherichia coli in administration after seven days, continues administration 3 days.Medication: administration fasting in preceding 4 hours, dip in normal saline with bare headed Glass rod earlier before the administration simultaneously and insert anus, the abundant defecation of stimulation in rats.Then, suppository is pressed dosed administration shown in the table 8, and filled in anus 4 hours, every day 1 time, successive administration 10 days with spongy embolus.
The observation of prostate lecithin: the full visual field of lecithin: ++ ++; The lecithin 3/4 full visual field: +++; The lecithin 1/2 full visual field: ++; The lecithin 1/4 full visual field :+.
Experimental result:
Influence to experimental rat prostate anatomic form
Normal group prostate surface is ruddy glossy, and gland tissue is soft and flexible, does not have adhesion with surrounding tissue, is easy to separate; The extensive adhesion of model group body of gland and surrounding tissue, the canescence lamellar tuberosity that as seen more or less, elasticity is relatively poor, and volume is more normally organized little.The suppository of wild chrysanthemum flower matched group, body of gland is glossy, color and luster is light slightly, softness still can, no tuberosity.4 rat bodies of gland and the slight adhesion of surrounding tissue are wherein arranged; The slight adhesion of small dose group and surrounding tissue, visible a small amount of canescence lamellar tuberosity, elasticity is poor slightly, and volume is more normally organized slightly little; Middle dosage group, the gland tissue softness, with the slight adhesion of surrounding tissue, size changes not obvious; Heavy dose of group, the gland tissue softness is glossy, and elasticity is better, does not have adhesion with surrounding tissue, and size changes not obvious, no tuberosity.
To lecithin, leukocytic influence in the experimental rat massage of prostate liquid
Lecithin almost is covered with the visual field in the normal prostatic, and the garden is spherical, drips similarly, shinny to fat, and refractivity is strong, is evenly distributed, and it differs in size.During prostatitis, lecithin reduces, and trend in heaps is arranged.This is that during hyperphlogosis, leukocyte almost is covered with the visual field, is the blister shape because during inflammation due to a large amount of lipids of macrophage phagocytic, no leukocyte in the normal prostatic during prostate light inflammation, has small quantities of leukocytes.The results are shown in Table 8,9.
Table 8 Herba Saussureae Involueratae prostate suppository is to (the X ± SD) of the prostatitic influence of rat chronic due to the escherichia coli
Model group and normal group compare,
△ △ △P＜0.01; Administration group and model group compare,
* *P＜0.01.
Table 9 Herba Saussureae Involueratae prostate suppository is to the influence of bacterial prostatitis due to the escherichia coli (X ± SD)
Model group and normal group compare,
△ △ △P＜0.01; Administration group and model group compare,
* *P＜0.01,
*P＜0.05,
*P＞0.05.
From table 8,9 as seen, the Herba Saussureae Involueratae prostate suppository is little, in, behind big three kinds of dosage and the positive control drug rectally, rat chronic prostatitis due to the escherichia coli is had the obvious treatment effect, positive control drug Flos Chrysanthemi Indici suppository also has significant therapeutical effect.
2. the Herba Saussureae Involueratae prostate suppository is to the experimentation of nonbacterial prostatitis influence
Experimental technique: choose 60 of Wistar rats, body weight 240-280g is divided into dosage group (V) in normal group (I), model group (II), Flos Chrysanthemi Indici suppository group (III), Herba Saussureae Involueratae prostate suppository small dose group (IV), the Herba Saussureae Involueratae prostate suppository, the heavy dose of group of Herba Saussureae Involueratae prostate suppository (VI) according to the body weight equilibrium.
Except that the normal control group, all the other 5 groups of rats after waiting to anaesthetize, are wiped out lower abdomen by hair all with etherization, the abdomen median line is cut down stomach wall open in the aseptic condition lower edge, mention bladder and both sides seminal vesicle gently with tweezers, expose the prostate notopodium invest the seminal vesicle inboard (the prostate perimeter region that is equivalent to the people, and people's prostatitis mainly betides perimeter region), inject each 0.1ml of XIAOZHILING ZHUSHEYE respectively in both sides, send attached internal organs, sew up abdominal wall muscle skin, put back to mouse cage.After the modeling, it is relatively poor that each organizes the rat mental status, and movable the minimizing is off one's feed, but all progressively recover in postoperative beginning in the 3rd day.Observed seven days continuously, each organizes none death of rat, and wound healing is good, and the mental status can be good, and hair color is moist, and appetite, two is just no abnormal.
Medication: each organizes rat in postoperative beginning in the 7th day administration.Medication: dip in normal saline with bare headed Glass rod earlier before the administration fasting in preceding 4 hours, administration and insert anus, the abundant defecation of stimulation in rats.Then, suppository is according to dosage filled in rectum, and block, every day 1 time, successive administration one month with the sterilization spongy embolus.
Prostate lecithin and leukocytic observation:
The full visual field of lecithin: ++ ++; The lecithin 3/4 full visual field: +++; The lecithin 1/2 full visual field: ++; The lecithin 1/4 full visual field :+;
Experimental result:
Influence to experimental rat prostate anatomic form
Normal group prostate surface is ruddy glossy, and gland tissue is soft and flexible, does not have adhesion with surrounding tissue, is easy to separate; The extensive adhesion of model group body of gland and surrounding tissue, the canescence lamellar tuberosity that as seen more or less, elasticity is relatively poor, and volume is more normally organized little.The suppository of wild chrysanthemum flower matched group, body of gland is glossy, color and luster is light slightly, softness still can, no tuberosity.The slight adhesion of small dose group and surrounding tissue, visible a small amount of canescence lamellar tuberosity, elasticity is poor slightly, and volume is more normally organized slightly little; Middle dosage group, the gland tissue softness, with the slight adhesion of surrounding tissue, size changes not obvious; Heavy dose of group, the gland tissue softness is glossy, and elasticity is better, does not have adhesion with surrounding tissue, and size changes not obvious, no tuberosity.
To lecithin and leukocytic influence in the experimental rat massage of prostate liquid
Lecithin almost is covered with the visual field in the normal prostatic, and the garden is spherical, drips similarly, shinny to fat, and refractivity is strong, is evenly distributed, and it differs in size.During prostatitis, lecithin reduces, and trend in heaps is arranged.This is that during hyperphlogosis, leukocyte almost is covered with the visual field, is the blister shape because during inflammation due to a large amount of lipids of macrophage phagocytic, no leukocyte in the normal prostatic during prostate light inflammation, has small quantities of leukocytes.The results are shown in Table 10,11.
Table 10 Herba Saussureae Involueratae prostate suppository is to the influence of nonbacterial prostatitis (X ± SD)
Model group and normal group compare,
△ △ △P＜0.01; Administration group and model group compare,
* *P＜0.01.
Table 11 Herba Saussureae Involueratae prostate suppository is to the influence of nonbacterial prostatitis (X ± SD)
Model group and normal group compare,
△ △ △P＜0.01; Administration group and model group compare,
* *P＜0.01,
*P＜0.05,
*P＞0.05.
From table 10,11 as seen, the Herba Saussureae Involueratae prostate suppository is little, in, behind big three kinds of dosage and the positive control drug rectally, the Herba Saussureae Involueratae prostate suppository is to the effect of nonbacterial prostatitis obvious suppression in heavy dose, and positive control drug Flos Chrysanthemi Indici suppository also has significant inhibitory effect.
The result shows, Herba Saussureae Involueratae prostate suppository mice (0.5,1,2g/kg), and rat (0.4,0.8,1.6g/kg) rectally suppresses dimethylbenzene induced mice auricular concha swelling (with model group P＜0.01 relatively); Antagonism acetic acid induced mice abdominal cavity capillary permeability increases (comparing P＜0.01 with model group); Suppress carrageenin induced mice pedal swelling (comparing P＜0.01) with model group; Rat fever reaction due to the dry yeast there is antagonism (with model group P＜0.01 relatively); Writhing method and hot plate method cause on the model of pain simultaneously, reduce the writhing response number of times of mice and improve the threshold of pain (comparing P＜0.01 with model group); To showing the infiltration that suppresses inflammatory cell on the scorching animal model of bacillary rat prostate, improve prostate secretory function and anti-fibroplasia effect (comparing P＜0.01) with model group; The infiltration that suppresses inflammatory cell be can show on the scorching animal model of non-bacterial rat prostate, prostate secretory function and anti-fibroplasia effect (comparing P＜0.01) improved with model group.Above-mentioned test explanation Herba Saussureae Involueratae prostate suppository has tangible antiinflammatory, analgesia, analgesic, detumescence, fibrosis hypertrophy, improve effect such as glandular secretion function.
Acute toxicity test;
Herba Saussureae Involueratae prostate suppository clinical administration approach is a rectally, we think that rectally can not guarantee that medicine is in intrarectal long-time stop in the acute toxicity test in mice process, medicine can not fully absorb, the toxic reaction that shows can not demonstrate fully the toxic reaction of medicine, therefore we select its mouse oral route of administration and rat rectally approach to carry out the prostate suppository acute toxicity test simultaneously, the target organ that observation toxic reaction that animal produced and toxic reaction may occur, for the detection of clinical toxicity provides reference frame, for long term toxicity test provides information and reference frame.(1) the oral acute toxicity test of mice: select KM kind mice, irritate the mode of stomach (ig) administration and carry out oral acute toxicity test, give Herba Saussureae Involueratae prostate suppository extractum liquid with Cmax 750mg/ml, the disposable filling stomach of maximum volume 0.4ml/10g → 148g (crude drug)/kg, observe the toxic reaction and the death condition of mice appearance in 14 days continuously.The result shows, Herba Saussureae Involueratae prostate suppository extractum liquid gastric infusion, and MTD＞148g (crude drug)/kg, the clinical plan of behaving is not seen drug-induced toxic reaction, the i.e. oral avirulence of this medicine this moment with 1187 times of dosage.(2) rectum acute toxicity test: select the SD rat to carry out the rectum acute toxicity test, with 50 times of Herba Saussureae Involueratae prostate suppository extractum liquid clinical dosage (35g crude drug/kg), concentration (709mg/ml), the disposable rectum of the maximum rectally volume (1ml/100g) of rat gives rat, observes the toxic reaction and the death condition of rat appearance in 14 days continuously.The result shows, Herba Saussureae Involueratae prostate suppository extractum liquid rat single rectally, and MTD＞35g crude drug/kg does not see drug-induced toxic reaction and death condition, Herba Saussureae Involueratae prostate suppository extractum liquid single rectally avirulence under this dosage.
(1), the oral acute toxicity test of mice
Summary: after [purpose] investigates Herba Saussureae Involueratae prostate suppository single-dose, the target organ that toxic reaction that animal produced and toxic reaction may occur.Measure the acute toxicity parameter of Herba Saussureae Involueratae prostate suppository, for long term toxicity test provides information and reference frame to mice.[method] selects KM kind mice, irritates the mode of stomach (ig) administration and carries out oral acute toxicity test, with Cmax, the disposable Herba Saussureae Involueratae prostate suppository extractum liquid that gives of maximum volume, observes the toxic reaction and the death condition of mice appearance in 14 days continuously.[result] mice Herba Saussureae Involueratae prostate suppository extractum liquid gastric infusion, MTD＞148g (crude drug)/kg, the clinical plan of behaving is not seen drug-induced toxic reaction this moment with 1187 times of dosage.
Test method: through the qualified mice of quarantining, fasting be can't help water 12 hours before test, selected body weight 20 ± 2g, 40, divided matched group and administration group at random, 20 every group.End fasting in 2 hours after the administration.Observed 14 days continuously, observe the mouse toxicity response situation, the itemized record symptom occurs, increases the weight of, alleviates or the time of death and the size of animal of appearance reaction, calculates the animal dead rate.Put to death surviving animals on the 14th day, dissect, perusal internal organs situation is calculated the multiple of the clinical plan of MTD value and people with dosage.
Experimental result: dead mouse situation and MTD
The dead mouse situation
The oral acute toxicity test animal dead of table 1 Herba Saussureae Involueratae prostate suppository extractum liquid mice situation
MTD result of calculation
Tolerance dose 2.96g (crude drug)/20g of every mice, thus can calculate MTD=148g (crude drug)/kg.
Body weight:
The oral acute toxicity test body weight of table 2 Herba Saussureae Involueratae prostate suppository extractum liquid mice table (X ± SD)
Administration group and matched group compare, P＞0.05.
Table 3, the oral acute toxicity test body weight change of Herba Saussureae Involueratae prostate suppository extractum liquid mice be table (X ± SD) as a result
Administration group and matched group compare, P＞0.05.
Conclusion: Herba Saussureae Involueratae prostate suppository extractum liquid mice oral administration acute toxicity parameter: under this experiment condition, Herba Saussureae Involueratae prostate suppository extractum liquid oral administration records the maximum tolerated dose multiple and behaves clinical plan with 1187 times of dosage, MTD＞148g (crude drug)/kg, oral avirulence.Toxic reaction: toxic reaction mainly shows as transient activity to be reduced, and promptly recovers normal after 2 hours.
(2), rat rectally acute toxicity test
Summary: after [purpose] investigated Herba Saussureae Involueratae prostate suppository rectum single-dose, the target organ that toxic reaction that animal produced and toxic reaction may occur was for the detection of clinical toxicity provides reference frame.Measure the acute toxicity parameter of Herba Saussureae Involueratae prostate suppository, provide reference frame for calculating new drug relative toxicity parameter and long term toxicity, cumulative toxicity and specific toxicity test dose to rat.
[method] selects the SD rat to carry out rectum single-dose toxicity test, and rat is with 50 times, the disposable Herba Saussureae Involueratae prostate suppository extractum liquid that gives of maximum volume of clinical dosage, the toxic reaction and the death condition of observing rat appearance in 14 days continuously.[result] Herba Saussureae Involueratae prostate suppository extractum liquid rat rectally, MTD＞35g crude drug/kg does not see drug-induced toxic reaction, pathological anatomy is not seen the obvious pathological change of Rats Organs and Tissues.
Test method: the SD rat through quarantining qualified, select body weight 220-250g, 20, be divided into matched group and administration group at random, 10 every group.Fasting be can't help water after 12 hours before experiment, and spongy embolus is filled in the rat internal rectum, inserted the perfusion syringe needle from the rectum edge, and slowly pushing syringe makes medicinal liquid soak into spongy embolus, end fasting in 2 hours after the administration.Timing immediately after the administration, the reaction that observe at once, 2h, 4h, 6h animal occurs.Tested the 2nd day to the 14th day, every morning observes once, and observe once afternoon, and the record toxic reaction occurs, increases the weight of, alleviates or the time of death and the size of animal of appearance reaction, calculates the animal dead rate.Before taking by weighing administration, observe mid-term, observe the weight of animals of tailend.Put to death animal on the 14th day, analyse, the perusal mucous membrane of rectum changes each tissue, organ disease situation.If pathological changes person is arranged, use 15% formalin fixed, the routine paraffin wax embedded section, HE dyeing, the light border is checked.
Experimental result:
The rats death situation
Table 1 Herba Saussureae Involueratae prostate suppository extractum liquid rectum acute toxicity test animal dead situation
Body weight
Table 2 Herba Saussureae Involueratae prostate suppository extractum liquid rat rectum acute toxicity test body weight observation table (X ± SD)
Administration group and matched group compare, P＞0.05.
Table 3 Herba Saussureae Involueratae prostate suppository extractum liquid rat rectum acute toxicity test body weight change is table (X ± SD) as a result
Administration group and matched group compare, P＞0.05.
Conclusion:
Herba Saussureae Involueratae prostate suppository extractum liquid rat rectally acute toxicity parameter: under this experiment condition, Herba Saussureae Involueratae prostate suppository extractum liquid rat rectally, MTD＞35g crude drug/kg, Herba Saussureae Involueratae prostate suppository extractum liquid single rectally avirulence.Toxic reaction: toxic reaction mainly shows as movable the minimizing on clinical sign, recovers normal after 2 hours, to not obviously influence of rat body weight.Pathological anatomy does not see that internal organs have tangible drug-induced pathological change.
Long term toxicity test:
Herba Saussureae Involueratae prostate suppository 8.4g, 16.8g, 33.6g (crude drug)/Kg (be equivalent to intend recommend clinical dosage 12,24,48 times) irritate stomach and give rat, and negative control group is irritated stomach and given equal-volume 0.5%CMC-Na.Be administered once every day, and after administration 3 months and 6 months, get 13 rats for every group, hematology, blood biochemical, blood clotting, blood electrolyte index are measured in the ventral aorta blood sampling, carry out after rat is put to death that system becomes celestial and histopathological examination.Each group residue rat is discontinued medication, and continues to feed and observes, and gets blood after 1 month and puts to death, and carries out The reversible experiment.The result shows, from the hematological indices check result as seen, mid-term: each dosage group and negative control group relatively, every index there are no significant difference (P＞0.05).Latter stage: the mean corpuscular hemoglobin concentration (MCHC) of three dosage groups (MCHC) and negative control group relatively all have significant difference (P＜0.05), all the other every indexs there are no significant difference (P＞0.05).Convalescent period: each dosage group and negative control group relatively, every index there are no significant difference (P＞0.05); Learn the index check result as seen from blood biochemical, mid-term: high dose group creatine kinase (CK) relatively has a declining tendency with negative control group, and significant difference (P＜0.05) is arranged; Low dose group triglyceride (TG) and the negative control group trend that relatively significantly decreases has significant difference (P＜0.01).All the other every indexs there are no significant difference (P＞0.05).Latter stage: each dosage group and negative control group relatively, every index there are no significant difference (P＞0.05).Convalescent period: each dosage group and negative control group relatively, every index there are no significant difference (P＞0.05); From blood electrolyte index check result as seen, mid-term: each dosage group and negative control group relatively, every index there are no significant difference (P＞0.05).Latter stage: each dosage group and negative control group relatively, every index there are no significant difference (P＞0.05).Convalescent period: each dosage group and negative control group relatively, every index there are no significant difference (P＞0.05); From blood clotting index check result as seen, mid-term: each dosage group and negative control group relatively, every index there are no significant difference (P＞0.05).Latter stage: each dosage group and negative control group relatively, every index there are no significant difference (P＞0.05).Convalescent period: each dosage group and negative control group relatively, every index there are no significant difference (P＞0.05).The histopathological examination result shows that mid-term, latter stage, convalescent period are respectively organized organs and tissues pathological examination no abnormality seen; Each dosage treated animal main organs does not find that all tangible drug-induced infringement sexually revises.
Experimental technique and result:
Test grouping: rat is weighed, and is divided into 4 groups at random with the body weight equilibrium, i.e. negative control group, low dosage administration group, middle dosed administration group and high dose administration group, 40 every group.
Medication: ig once a day, amounts to 6 months.The clinical administration approach is a rectally, we think that rectally can not guarantee that medicine is in the intrarectal time of staying in the rat repetitively administered toxicity test process, the toxic reaction that shows can not demonstrate fully the toxic reaction of medicine, so we select oral administration.
The mucous membrane of rectum irritation test
Select the SD rat to carry out Herba Saussureae Involueratae prostate suppository rectum multiple dosing irritation test, rat rectally dosage is the clinical application amount, and establish vehicle group and blank group, successive administration and observing 7 days behind the last administration 24h, is analysed 6 animals for every group, take out the rectum local organization, observe mucous membrane of rectum and have or not situations such as hyperemia, redness, judge with the integral estimation method whether medicine has zest to mucous membrane of rectum, and carry out histopathological examination.If check result has unusually, then the animal of drug withdrawal observation need be cooked histopathological examination; If check result is no abnormal, then the animal of drug withdrawal observation need not cooked histopathological examination.The result shows: the Herba Saussureae Involueratae prostate suppository is repeatedly behind the rectally, zootomy is not seen phenomenons such as drug-induced mucous membrane of rectum hyperemia, redness, judge medicine to the mucous membrane of rectum nonirritant with the integral estimation method, histopathological examination does not see that the rat mucous membrane of rectum has drug-induced pathological change.
Test method: through the qualified rat of quarantining, fasting be can't help water 4 hours before experiment, took by weighing every rat body weight, selected body weight 220-250g, 30, was divided into 3 groups at random, 10 every group.End fasting in 2 hours after the administration.Successive administration was observed 7 days, observed the rat toxicity response situation, and the itemized record symptom occurs, increases the weight of, alleviates or the time of death and the size of animal of appearance reaction, calculates the animal dead rate.The 8th day every group analyse 6 animals, the perusal mucous membrane of rectum has or not situations such as hyperemia, redness, judges with the integral estimation method whether medicine has zest to mucous membrane of rectum, and makes histopathological examination.If check result has unusually, then the animal of reversibility observation need be cooked histopathological examination; If check result is no abnormal, then the animal of drug withdrawal observation only need be cooked and analyse observation.Reversibility observing time is 7 days (every group of 4 rats).
Experimental result:
Clinical sign: the Herba Saussureae Involueratae prostate suppository transient activity occurs and reduces after repeatedly rectum gives rat when administration, promptly recovers normal after 2 hours, finishes not see that other toxic reaction is arranged up to the observation period.
The rats death situation
Table 1 Herba Saussureae Involueratae prostate suppository mucous membrane of rectum irritation test rats death situation
Body weight
Table 2 Herba Saussureae Involueratae prostate suppository mucous membrane of rectum irritation test body weight table (X ± SD)
Herba Saussureae Involueratae prostate suppository group and vehicle group and blank group compare, P＞0.05.
Zootomy, integral estimation and histopathological examination
Zootomy is not seen phenomenons such as drug-induced mucous membrane of rectum hyperemia, redness, judges medicine to the mucous membrane of rectum nonirritant with the integral estimation method, and histopathological examination does not see that the rat mucous membrane of rectum has drug-induced pathological change.
Conclusion: under this experiment condition, Herba Saussureae Involueratae prostate suppository rectally is to rat mucous membrane of rectum nonirritant.Zootomy is not seen phenomenons such as drug-induced mucous membrane of rectum hyperemia, redness, judges medicine to the mucous membrane of rectum nonirritant with the integral estimation method, and histopathological examination does not see that the rat mucous membrane of rectum has drug-induced pathological change.
The specific embodiment
The content of each component is among the embodiment 1 (per 1000 (piece)):
A, be ground into fine powder after will Olibanum 150g, Myrrha 150g concocting, and cross 100 mesh sieves, mixing is standby;
B, Rhizoma Curcumae 620g are ground into coarse powder, add 8 times of water gagings, extract volatile oil according to a conventional method 6 hours, and volatile oil device is in addition collected, and is standby;
C, medicinal residues and Herba Saussureae Involueratae 500g, Flos Carthami 350g, rhizoma sparganic 620g add 10 times of amount 80% alcohol reflux 3 times, each 1.5 hours, merging filtrate filters, and it is 1.15～1.20 clear paste that filtrate decompression is concentrated into relative density, vacuum drying gets dry extract, pulverize, mixes mistake 100 mesh sieves with fine powder after Olibanum, Myrrha are pulverized, as mixed powder, standby;
D, Radix Arnebiae (Radix Lithospermi) 1100g add 12 times of amount 95% ethanol and carry out percolation with the speed of per minute 7ml, collect percolate, and be to thick paste, standby at decompression recycling ethanol below 60 ℃;
E, take by weighing S by the amount of 2.3 times of mixed powders
36Mixed fatty glycerides 1.50kg and 1% sodium lauryl sulphate 15g, be heated to molten condition, add mixed powder and thick paste, fully stir evenly, the volatile oil that adds during to 45 ℃ to be cooled, continue to be stirred to evenly, inject the bolt mould, take out the cooling back, make 1000 (piece), every 2.2g, or make paste according to a conventional method and get final product.Said preparation prevents, treats the purposes of the external used medicine of acute and chronic prostatitis, prostatic hyperplasia (hypertrophy) as preparation.
Using method: preceding the rising with morning of sleeping filled in medicine anus 4-6 centimeters after an action of the bowels, and every day 2 times, each one piece or one, one month is a course of treatment.
The content of each component is among the embodiment 2 (per 1000 (piece)):
A, be ground into fine powder after will Olibanum 160g, Myrrha 160g concocting, and cross 100 mesh sieves, mixing is standby;
B, Rhizoma Curcumae 670g are ground into coarse powder, add 8 times of water gagings, extract volatile oil according to a conventional method 6 hours, and volatile oil device is in addition collected, and is standby;
C, medicinal residues and Herba Saussureae Involueratae 580g, Flos Carthami 380g, rhizoma sparganic 680g add 10 times of amount 80% alcohol reflux 3 times, each 1.5 hours, merging filtrate filters, and it is 1.15～1.20 clear paste that filtrate decompression is concentrated into relative density, vacuum drying gets dry extract, pulverize, mixes mistake 100 mesh sieves with fine powder after above-mentioned Olibanum, Myrrha are pulverized, as mixed powder, standby;
D, Radix Arnebiae (Radix Lithospermi) 1140g add 12 times of amount 95% ethanol and carry out percolation with the speed of per minute 7ml, collect percolate, and be to thick paste, standby at decompression recycling ethanol below 60 ℃;
E, take by weighing S by the amount of 2.3 times of mixed powders
36Mixed fatty glycerides 1.80kg and 1% sodium lauryl sulphate 18g; be heated to molten condition; add mixed powder and thick paste, fully stir evenly, the volatile oil that adds during to 45 ℃ to be cooled; continue to be stirred to evenly; inject the bolt mould, take out the cooling back, make 1000 or piece; every 2.2g, or make paste according to a conventional method and get final product.Said preparation prevents, treats the purposes of the external used medicine of acute and chronic prostatitis, prostatic hyperplasia (hypertrophy) as preparation.
Using method: preceding the rising with morning of sleeping filled in medicine anus 4-6 centimeters after an action of the bowels, and every day 2 times, each one piece or one, one month is a course of treatment.
The content of each component is among the embodiment 3 (per 1000 (piece)):
A, be ground into fine powder after will Olibanum 180g, Myrrha 180g concocting, and cross 100 mesh sieves, mixing is standby;
B, Rhizoma Curcumae 640g are ground into coarse powder, add 8 times of water gagings, extract volatile oil according to a conventional method 6 hours, and volatile oil device is in addition collected, and is standby;
C, medicinal residues and Herba Saussureae Involueratae 530g, Flos Carthami 370g, rhizoma sparganic 650g add 10 times of amount 80% alcohol reflux 3 times, each 1.5 hours, merging filtrate filters, and it is 1.15～1.20 clear paste that filtrate decompression is concentrated into relative density, vacuum drying gets dry extract, pulverize, mixes mistake 100 mesh sieves with fine powder after Olibanum, Myrrha are pulverized, as mixed powder, standby;
D, Radix Arnebiae (Radix Lithospermi) 1130g add 12 times of amount 95% ethanol and carry out percolation with the speed of per minute 7ml, collect percolate, and be to thick paste, standby at decompression recycling ethanol below 60 ℃;
E, take by weighing S by the amount of 2.3 times of mixed powders
36Mixed fatty glycerides 1.80kg and 1% sodium lauryl sulphate 18g; be heated to molten condition; add mixed powder and thick paste, fully stir evenly, the volatile oil that adds during to 45 ℃ to be cooled; continue to be stirred to evenly; inject the bolt mould, take out the cooling back, make 1000 or piece; every 2.2g, or make paste according to a conventional method and get final product.Said preparation prevents, treats the purposes of the external used medicine of acute and chronic prostatitis, prostatic hyperplasia (hypertrophy) as preparation.
Using method: preceding the rising with morning of sleeping filled in medicine anus 4-6 centimeters after an action of the bowels, and every day 2 times, each one piece or one, one month is a course of treatment.
The content of each component is among the embodiment 4 (per 1000 (piece)):
A, be ground into fine powder after will Olibanum 200g, Myrrha 200g concocting, and cross 100 mesh sieves, mixing is standby;
B, Rhizoma Curcumae 690g are ground into coarse powder, add 8 times of water gagings, extract volatile oil according to a conventional method 6 hours, and volatile oil device is in addition collected, and is standby;
C, medicinal residues and Herba Saussureae Involueratae 600g, Flos Carthami 400g, rhizoma sparganic 690g add 10 times of amount 80% alcohol reflux 3 times, each 1.5 hours, merging filtrate filters, and it is 1.15～1.20 clear paste that filtrate decompression is concentrated into relative density, vacuum drying gets dry extract, pulverize, mixes mistake 100 mesh sieves with fine powder after Olibanum, Myrrha are pulverized, as mixed powder, standby;
D, Radix Arnebiae (Radix Lithospermi) 1150g add 12 times of amount 95% ethanol and carry out percolation with the speed of per minute 7ml, collect percolate, and be to thick paste, standby at decompression recycling ethanol below 60 ℃;
E, take by weighing S by the amount of 2.3 times of mixed powders
36Mixed fatty glycerides 2.0kg and 1% sodium lauryl sulphate 20g; be heated to molten condition; add mixed powder and thick paste, fully stir evenly, the volatile oil that adds during to 45 ℃ to be cooled; continue to be stirred to evenly; inject the bolt mould, take out the cooling back, make 1000 or piece; every 2.2g, or make paste according to a conventional method and get final product.Said preparation prevents, treats the purposes of the external used medicine of acute and chronic prostatitis, prostatic hyperplasia (hypertrophy) as preparation.
Using method: preceding the rising with morning of sleeping filled in medicine anus 4-6 centimeters after an action of the bowels, and every day 2 times, each one piece or one, one month is a course of treatment.
Claims (3)
1. the medicine for external use snow lotus preparation for treating prostate disease of a control acute and chronic prostatitis, prostatic hyperplasia, prostate hyperplasia genitourinary system; it is characterized in that said preparation is by crude drug Herba Saussureae Involueratae 500-600g, Flos Carthami 350-400g, Radix Arnebiae (Radix Lithospermi) 1100-1150g, Rhizoma Curcumae 620-690g, rhizoma sparganic 620-690g, Olibanum 150200g and Myrrha 150-200g; adjuvant is that mixed fatty glycerides 1.40-1.80kg and 1% sodium lauryl sulphate 15-20g make, with per 1000 or piece be radix.
2. the preparation method of snow lotus preparation for treating prostate disease according to claim 1 is characterized in that following these steps to carrying out:
A, will Olibanum, Myrrha is ground into fine powder after concocting, and crosses 100 mesh sieves, mixing is standby;
B, Rhizoma Curcumae are ground into coarse powder, add 8 times of water gagings, extract volatile oil according to a conventional method 6 hours, and volatile oil device is in addition collected, and is standby;
C, medicinal residues and Herba Saussureae Involueratae, Flos Carthami, rhizoma sparganic add 10 times of amount 80% alcohol reflux 3 times, each 1.5 hours, merging filtrate filters, and it is 1.15～1.20 clear paste that filtrate decompression is concentrated into relative density, vacuum drying gets dry extract, pulverize, mixes mistake 100 mesh sieves with fine powder after above-mentioned Olibanum, Myrrha are pulverized, as mixed powder, standby;
D, Radix Arnebiae (Radix Lithospermi) add 12 times of amount 95% ethanol and carry out percolation with the speed of per minute 7ml, collect percolate, and be to thick paste, standby at decompression recycling ethanol below 60 ℃;
E, take by weighing S by the amount of 2.3 times of mixed powders
36Mixed fatty glycerides contains 1% sodium lauryl sulphate; be heated to molten condition; add mixed powder and thick paste, fully stir evenly, the volatile oil that adds during to 45 ℃ to be cooled; continue to be stirred to evenly; inject the bolt mould, take out the cooling back, make 1000 or piece; every 2.2g, or make paste according to a conventional method and get final product.
The described snow lotus preparation for treating prostate disease of claim 1 in preparation prevention, treat the purposes aspect the external used medicine of acute and chronic prostatitis, prostatic hyperplasia, prostate hyperplasia.
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| CNB2006100842950A CN100408085C (en) | 2006-05-30 | 2006-05-30 | Snow lotus preparation for treating prostate disease |
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| CNB2006100842950A CN100408085C (en) | 2006-05-30 | 2006-05-30 | Snow lotus preparation for treating prostate disease |
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| CN1383860A (en) * | 2002-05-21 | 2002-12-11 | 崔行善 | Medicine for treating bladder cancer |
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