CN104673482A - Extraction method of pseudo-ginseng oil - Google Patents
Extraction method of pseudo-ginseng oil Download PDFInfo
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- CN104673482A CN104673482A CN201310645752.9A CN201310645752A CN104673482A CN 104673482 A CN104673482 A CN 104673482A CN 201310645752 A CN201310645752 A CN 201310645752A CN 104673482 A CN104673482 A CN 104673482A
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Abstract
The invention relates to an extraction method of pseudo-ginseng oil. The extraction method changes a traditional crushing and water-alcohol extraction method, and implements extraction by using superfinishing and supercritical carbon dioxide extraction method; and the extraction method comprises the following steps: (1) pretreatment; (2) primary extraction; (3) alkalization; 4) acidification; and (5) extraction. The extraction method both can improve the extraction rate of pseudo-ginseng oil, and can reduce the production cost, thereby providing raw material guarantee for the development of multiple pseudo-ginseng oil dosage forms.
Description
Technical field
The invention belongs to field of veterinary, especially a kind of extracting method of pseudo-ginseng oil.
Background technology
Pseudo-ginseng, perennial herb, China among the people be used for treatment fall flutter that the stasis of blood is swollen, obstruction of qi in the chest angina, blood stasis through closing, dysmenorrhoea, postpartum stasis of blood the moon stomachache and sore, carbuncle and painful swelling etc. in.Research shows that it contains plurality of active ingredients, wherein main is pseudo-ginseng oil, the effect such as it has antibacterial hemostasis, it is cardiovascular to regulate and immunity of organism is machine-processed, being widely used in medicine, food and chemical field, is a kind of lead compound having potential Development volue.
Present stage, pseudo-ginseng oil more perfect extraction scheme, so the present invention mainly aims to the extraction process providing a kind of pseudo-ginseng oil, the oil yield of raising pseudo-ginseng oil, reduces production cost.Nowadays utilize the medicine such as microbiotic and hormone to carry out antibacterial phenomenon to grow with each passing day, but residual condition is serious in vivo, is unfavorable for the edible of animal byproduct, jeopardizes human body health.Pseudo-ginseng oil has numerous pharmacological actions such as antibacterial, hemostasis and pain-relieving, and drug effect better and shorten the time that medicine reaches blood peak concentration of drug.
Summary of the invention
The object of the present invention is to provide a kind of extracting method of pseudo-ginseng oil, change traditional extraction process, effectively can improve the content of pseudo-ginseng oil in the oil yield of pseudo-ginseng and pseudo-ginseng.Adopt this kind of extraction process that the extraction yield of pseudo-ginseng oil is raised, extraction time is shorter, and Extracting temperature is lower, and no solvent residue, can improve the utilization ratio of pseudo-ginseng oil.
The present invention is realized by following technology:
An extracting method for pseudo-ginseng oil, the step of its extracting method is as follows:
(1) pre-treatment is extracted: take a certain amount of pseudo-ginseng rhizome, add in shredder and carry out superfinishing, obtain abrasive material A, for subsequent use;
(2) carry at the beginning of: add ethyl acetate, just do not have gained abrasive material A in step (1), slective extraction temperature is 35-100 DEG C, and extracting pressure is 10-30MPa, and extraction time is 30-100min, and carbon dioxide flow is 10-30L/h, is extracted liquid B, for subsequent use;
(3) alkalize: be 0.25-10% sodium hydroxide solution continuous extraction 3 times by step (2) gained extraction liquid B 1-6 times amount concentration, collect upper strata oily liquids, obtain oily liquids C, for subsequent use;
(4) acidifying: the 2-8mol/L hcl acidifying dripping 2-5 times amount in the oily liquids C of step (3) gained, aobvious acid to blue litmus paper, obtain the liquid D after acidifying, for subsequent use;
(5) extract: by the 1-5 times amount extracted with diethyl ether 3 times of the liquid D after step (4) gained acidifying, after merging 3 extraction liquids, with anhydrous sodium sulfate drying, reclaim ether, namely obtain the oily finished product of pseudo-ginseng.
Advantage of the present invention and beneficial effect are:
1, the present invention is by the extraction process of the traditional pseudo-ginseng oil of improvement, substantially increases the extraction yield of pseudo-ginseng oil, and then increases the utilization ratio of pseudo-ginseng, increases economic benefit.
2, present invention process step is simple, reaction process is easy to control, good product quality, yield are high, and production cost is lower, is a kind of technique of novel extraction pseudo-ginseng oil.
3, pseudo-ginseng oil no solvent residue in leaching process, metabolism is complete in vivo, drug accumulation can not occur, play drug effect at short notice.
Embodiment
The present invention is described in further detail by following examples.It should be noted that: following embodiment is illustrative, is not determinate, can not limit protection scope of the present invention with following embodiment.
Pseudo-ginseng involved in the present invention commercially can be bought and obtain.
Embodiment 1
An extracting method for pseudo-ginseng oil, the step of its extracting method is:
(1) pre-treatment is extracted: take a certain amount of pseudo-ginseng rhizome, add in shredder and carry out superfinishing, obtain abrasive material A, for subsequent use;
(2) carry at the beginning of: add ethyl acetate, just do not have gained abrasive material A in step (1), slective extraction temperature is 35 DEG C, and extracting pressure is 10MPa, and extraction time is 30min, and carbon dioxide flow is 10L/h, is extracted liquid B, for subsequent use;
(3) alkalize: be 0.25% sodium hydroxide solution continuous extraction 3 times by step (2) gained extraction liquid B with 1 times amount concentration, collection upper strata oily liquids, obtains oily liquids C, for subsequent use;
(4) acidifying: the 2mol/L hcl acidifying dripping 2 times amount in the oily liquids C of step (3) gained, aobvious acid to blue litmus paper, obtain the liquid D after acidifying, for subsequent use;
(5) extract: by the 1 times amount extracted with diethyl ether 3 times of the liquid D after step (4) gained acidifying, after merging 3 extraction liquids, with anhydrous sodium sulfate drying, recovery ether, namely obtain the oily finished product of pseudo-ginseng.
Embodiment 2
An extracting method for pseudo-ginseng oil, the step of its extracting method is:
(1) pre-treatment is extracted: take a certain amount of pseudo-ginseng rhizome, add in shredder and carry out superfinishing, obtain abrasive material A, for subsequent use;
(2) carry at the beginning of: add ethyl acetate, just do not have gained abrasive material A in step (1), slective extraction temperature is 75 DEG C, and extracting pressure is 20MPa, and extraction time is 70min, and carbon dioxide flow is 20L/h, is extracted liquid B, for subsequent use;
(3) alkalize: be 5% sodium hydroxide solution continuous extraction 3 times by step (2) gained extraction liquid B with 3 times amount concentration, collection upper strata oily liquids, obtains oily liquids C, for subsequent use;
(4) acidifying: the 5mol/L hcl acidifying dripping 4 times amount in the oily liquids C of step (3) gained, aobvious acid to blue litmus paper, obtain the liquid D after acidifying, for subsequent use;
(5) extract: by the 3 times amount extracted with diethyl ether 3 times of the liquid D after step (4) gained acidifying, after merging 3 extraction liquids, with anhydrous sodium sulfate drying, recovery ether, namely obtain the oily finished product of pseudo-ginseng.
Embodiment 3
An extracting method for pseudo-ginseng oil, the step of its extracting method is:
(1) pre-treatment is extracted: take a certain amount of pseudo-ginseng rhizome, add in shredder and carry out superfinishing, obtain abrasive material A, for subsequent use;
(2) carry at the beginning of: add ethyl acetate, just do not have gained abrasive material A in step (1), slective extraction temperature is 100 DEG C, and extracting pressure is 30MPa, and extraction time is 100min, and carbon dioxide flow is 30L/h, is extracted liquid B, for subsequent use;
(3) alkalize: be 10% sodium hydroxide solution continuous extraction 3 times by step (2) gained extraction liquid B with 6 times amount concentration, collection upper strata oily liquids, obtains oily liquids C, for subsequent use;
(4) acidifying: the 8mol/L hcl acidifying dripping 5 times amount in the oily liquids C of step (3) gained, aobvious acid to blue litmus paper, obtain the liquid D after acidifying, for subsequent use;
(5) extract: by the 5 times amount extracted with diethyl ether 3 times of the liquid D after step (4) gained acidifying, after merging 3 extraction liquids, with anhydrous sodium sulfate drying, recovery ether, namely obtain the oily finished product of pseudo-ginseng.
Pseudo-ginseng oil-contg measures
1.1 thin-layer chromatographys detect
One end pencil drawing zero line on thin layer plate, evenly drops in the present invention and pseudo-ginseng oil standard product solution on zero line respectively with kapillary, airing or dry up rearmounted thin layer plate in the separation chamber filling developping agent, and immersion depth is 0.5cm.When to be deployed dose of forward position is about near 1cm far from top, taken out by chromatosheet, after dry, spray is with developer, or develops the color under ultraviolet lamp.Observing the present invention is consistent with colour developing place of pseudo-ginseng oil standard product solution, illustrates in extracting solution of the present invention containing pseudo-ginseng oil.
1.2 high performance liquid chromatography carry out assay
Get the volumetric flask constant volume that pseudo-ginseng oil standard product solution 1ml, 2ml, 3ml, 4ml, 5ml are placed in 1000ml respectively, the solution after constant volume is designated as A, B, C, D, E group respectively.By above-mentioned 5 groups of solution by after filtering with microporous membrane, measure respectively by high performance liquid chromatography, the appearance time of record pseudo-ginseng oil and 5 groups of peak areas, and utilize the concentration of pseudo-ginseng oil standard product and peak area to do the typical curve of pseudo-ginseng oil solution.
Get the volumetric flask constant volume that extracting solution 1ml of the present invention is placed in 1000ml, solution is filtered by millipore filtration, solution after filtration injects high performance liquid chromatography and measures, according to its corresponding peak area of appearance time record of standard substance, the typical curve of contrast pseudo-ginseng oil solution, judges the content of pseudo-ginseng oil of the present invention.
1.3 the present invention and traditional water containing ethanol extraction pseudo-ginseng oil technics comparing
Pseudo-ginseng oil-contg measurement result in table 1 extracting solution
| Method | Pseudo-ginseng oil oil yield | Pseudo-ginseng oil-contg | Obtain phenol rate |
| Of the present invention group | 18.04% | 98.7% | 17.92% |
| Water extraction group | 6.52% | 60.5% | 5.7% |
| Ethanol-extracted group | 10.05 | 68.75 | 9.85% |
Adopt extracting method of the present invention, in pseudo-ginseng, the oil yield of pseudo-ginseng oil is 18.04%, in pseudo-ginseng oil, the content of pseudo-ginseng oil is 98.7%, obtaining phenol rate is 17.92%, and in the present invention, pseudo-ginseng oil solution is faint yellow, and oil yield is high, extraction time is shorter, Extracting temperature is lower, and no solvent residue, is obviously better than traditional extraction process.
Claims (1)
1. an extracting method for pseudo-ginseng oil, is characterized in that: the step of this extracting method is as follows:
(1) pre-treatment is extracted: take a certain amount of pseudo-ginseng rhizome, add in shredder and carry out superfinishing, obtain abrasive material A, for subsequent use;
(2) carry at the beginning of: add ethyl acetate, just do not have gained abrasive material A in step (1), slective extraction temperature is 35-100 DEG C, and extracting pressure is 10-30MPa, and extraction time is 30-100min, and carbon dioxide flow is 10-30L/h, is extracted liquid B, for subsequent use;
(3) alkalize: be 0.25-10% sodium hydroxide solution continuous extraction 3 times by step (2) gained extraction liquid B 1-6 times amount concentration, collect upper strata oily liquids, obtain oily liquids C, for subsequent use;
(4) acidifying: the 2-8mol/L hcl acidifying dripping 2-5 times amount in the oily liquids C of step (3) gained, aobvious acid to blue litmus paper, obtain the liquid D after acidifying, for subsequent use;
(5) extract: by the 1-5 times amount extracted with diethyl ether 3 times of the liquid D after step (4) gained acidifying, after merging 3 extraction liquids, with anhydrous sodium sulfate drying, reclaim ether, namely obtain the oily finished product of pseudo-ginseng.
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| CN201310645752.9A CN104673482A (en) | 2013-12-03 | 2013-12-03 | Extraction method of pseudo-ginseng oil |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106190547A (en) * | 2016-08-18 | 2016-12-07 | 贵州石阡佛顶山野生油茶油业有限公司 | A kind of solvent extraction method extracts the production method of Oleum Camelliae |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1468947A (en) * | 2003-06-20 | 2004-01-21 | 列 陈 | Glossy ganoderma spore oil extracting and refining process |
| CN1724005A (en) * | 2005-07-18 | 2006-01-25 | 天津大学 | Method for extracting volatile oil and liposoluble alkaloid from plumula nelumbini |
| CN101161085A (en) * | 2006-10-11 | 2008-04-16 | 马玉林 | Cold squeeze preparing technique of persic oil |
| CN101845362A (en) * | 2010-06-22 | 2010-09-29 | 中南林业科技大学 | Method for gathering oleic acid from tea-seed oil |
| CN102993041A (en) * | 2011-09-15 | 2013-03-27 | 汤敦明 | Staged extraction method for capsaicin |
-
2013
- 2013-12-03 CN CN201310645752.9A patent/CN104673482A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1468947A (en) * | 2003-06-20 | 2004-01-21 | 列 陈 | Glossy ganoderma spore oil extracting and refining process |
| CN1724005A (en) * | 2005-07-18 | 2006-01-25 | 天津大学 | Method for extracting volatile oil and liposoluble alkaloid from plumula nelumbini |
| CN101161085A (en) * | 2006-10-11 | 2008-04-16 | 马玉林 | Cold squeeze preparing technique of persic oil |
| CN101845362A (en) * | 2010-06-22 | 2010-09-29 | 中南林业科技大学 | Method for gathering oleic acid from tea-seed oil |
| CN102993041A (en) * | 2011-09-15 | 2013-03-27 | 汤敦明 | Staged extraction method for capsaicin |
Non-Patent Citations (2)
| Title |
|---|
| 李毛全 等: "超临界CO2萃取三七中挥发油及皂苷的工艺研究", 《安徽医药》 * |
| 瞿新华: "植物精油的提取与分离技术", 《安徽农业科学》 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106190547A (en) * | 2016-08-18 | 2016-12-07 | 贵州石阡佛顶山野生油茶油业有限公司 | A kind of solvent extraction method extracts the production method of Oleum Camelliae |
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Application publication date: 20150603 |