CN104664075A - Composite feed additive - Google Patents
Composite feed additive Download PDFInfo
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- CN104664075A CN104664075A CN201310637934.1A CN201310637934A CN104664075A CN 104664075 A CN104664075 A CN 104664075A CN 201310637934 A CN201310637934 A CN 201310637934A CN 104664075 A CN104664075 A CN 104664075A
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- feed additive
- composite feed
- composite
- potato starch
- sweet potato
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Abstract
The invention provides a composite feed additive. The composite feed additive is prepared from the following components in percentage by mass: 10-30% of streptococcus faecalis, 15-25% of bacillus subtilis, 10-25% of lactobacillus plantarum, 15-30% lactobacillus casei, 10-15% of sweet potato starch and the balance of shell powder. The composite feed additive is prepared through the steps of preparing strains by use of streptococcus faecalis, bacillus subtilis, lactobacillus plantarum and lactobacillus casei and then mixing the strains with sweet potato starch and shell powder.
Description
Technical field
The present invention relates to a kind of composite feed additive for domestic animals cultivation and preparation method thereof, belong to the technical field of biological materials that microbial modulator is applied in livestock breed aquatics.
Background technology
Along with the increase with livestock rearing density that further develops of herding intensive farm, the generation of various herding disease is more and more frequent, and the herding of most cultivation is all in sub-health state.Once widely used medicine not only made pathogenic microorganism create the serious resistance to the action of a drug, also caused the serious problems of livestock products medicament residue, and development goes down to affect the health of the mankind.Microorganism formulation arises at the historic moment under this historical background, and the sustainable development for animal husbandry provides effective, a reliable approach.Adopt biotechnology composite fermentation, utilize multiple-microorganism to interact, form the system of an ecological balance.The initial definition of feed addictive is feed supplement, to add the deficiency that the composition such as vitamin and inorganic salts fills up feed Middle nutrition composition, the nutrition of balanced feed, and obtains good feeding effect.
In recent years, microbe additive becomes focus as green feed additive.Microorganism forms advantage profitable strain by the biological competition repulsive interaction in animal alimentary canal, by strengthening the digest and decompose to feed, promoting growth of animal, improving feed coefficient.Therefore, scientific research personnel to this to research increasing.But in the suitability for industrialized production of reality, thalline effect is single, the strong again scale constraining microbial product of poor stability, industrialized development.
Summary of the invention
The object of the present invention is to provide a kind of composite feed additive for domestic animals cultivation and preparation method thereof.The present invention makes full use of the synergistic function between bacterial classification, plays and promotes that domestic animals are to food conversion, nutritional labeling absorptivity, thus promote growth of animal, reach the object of synergy volume increase.
For achieving the above object, the present invention adopts following technical scheme:
Composite feed additive, adopt streptococcus fecalis (Streptococcus faecalis), bacillus subtilis (Bacillus subtilis), Lactobacillus plantarum (Lactobacillus plantarum) and Lactobacillus casei (Lactobacillus casei) to prepare bacterial classification, then mix with sweet potato starch and oyster shell whiting.
Composite feed additive provided by the invention, the mass percent of described additive is: streptococcus fecalis 10 ~ 30%, bacillus subtilis 15 ~ 25%, Lactobacillus plantarum 10 ~ 25%, Lactobacillus casei 15 ~ 30%, sweet potato starch 10 ~ 15%, oyster shell whiting surplus.
Composite feed additive provided by the invention, preferably, the mass percent of described additive is: streptococcus fecalis 15 ~ 25%, bacillus subtilis 15 ~ 20%, Lactobacillus plantarum 15 ~ 20%, Lactobacillus casei 17 ~ 25%, sweet potato starch 10 ~ 12%, oyster shell whiting surplus.
Composite feed additive provided by the invention, further preferably, the mass percent of described additive is: streptococcus fecalis 24%, bacillus subtilis 18%, Lactobacillus plantarum 18%, Lactobacillus casei 20%, sweet potato starch 12%, oyster shell whiting 8%.
Present invention also offers the preparation method of above-mentioned composite feed additive, comprise the following steps:
1. inoculate: be connected on slant medium by described each bacterial classification, cultivation temperature 30 ~ 35 DEG C, incubation time is 36 ~ 48h;
2. Spawn incubation: the culture medium picking list bacterium colony grown is forwarded to and is equipped with in the 50mL conical flask of fluid nutrient medium, sterile working, 34 DEG C, 130r/min cultivates 36 ~ 48h;
3. one grade fermemtation: strain inoculation will be cultivated in the 500mL conical flask filling fluid nutrient medium, sterile working, 34 DEG C, 130r/min cultivates 36 ~ 48h;
4. mixed culture fermentation: each bacterial classification bacterium liquid is inoculated in the fermentation tank filling composite bacteria agent capable culture medium in proportion, composite bacteria agent capable medium pH 6.0 ~ 8.0, inoculum concentration is 1 ~ 5%, adds sweet potato starch and oyster shell whiting, cultivate 4 ~ 7 days at 34 DEG C, make bacterium number in zymotic fluid reach 10 ~ 15 × 10
8individual/mL, namely obtains described composite feed additive.
The preparation method of composite feed additive provided by the invention, described composite bacteria agent capable culture medium prescription is: honey 1 ~ 5g/100mL, sucrose 5 ~ 10g/100mL, MC peptone 1 ~ 5g/100mL, agar 0.1 ~ 0.8g/100mL, KH
2pO
40.2 ~ 0.5g/100mL, K
2hPO
40.5 ~ 3g/100mL, MgSO
47H
2o0.5 ~ 3g/100mL, NaCl0.5 ~ 10g/100mL, all the other are distilled water.
Compared to prior art, composite microbial feed additive provided by the invention adopts streptococcus fecalis, bacillus subtilis, Lactobacillus plantarum, Lactobacillus casei, sweet potato starch and oyster shell whiting mixed culture fermentation to be prepared from.Above bacterial classification is respectively through Spawn incubation, and two-stage fermentation, makes bacterium number in zymotic fluid reach 10 ~ 15 × 10
8individual/mL, obtains composite feed additive.This product is through verifying on the spot for a long time, and rationally, stable effect, can promote growth of animal to formula, improves animal product output, and improve survival rate, disease resistance, improves food conversion ratio, and improves product quality.
Detailed description of the invention
The following examples can make the present invention of those of ordinary skill in the art's comprehend, but do not limit the present invention in any way.
Embodiment 1
Composite feed additive, adopts streptococcus fecalis 30%, bacillus subtilis 15%, Lactobacillus plantarum 20%, Lactobacillus casei 15%, sweet potato starch 10%, oyster shell whiting 10%, prepares according to the following steps:
1. inoculate: be connected on slant medium by described each bacterial classification, cultivation temperature 34 DEG C, incubation time is 36h;
2. Spawn incubation: the culture medium picking list bacterium colony grown is forwarded to and is equipped with in the 50mL conical flask of fluid nutrient medium, sterile working, 34 DEG C, 130r/min cultivates 36h;
3. one grade fermemtation: strain inoculation will be cultivated in the 500mL conical flask filling fluid nutrient medium, sterile working, 34 DEG C, 130r/min cultivates 36h;
4. mixed culture fermentation: each bacterial classification bacterium liquid is inoculated in the fermentation tank filling composite bacteria agent capable culture medium in proportion, composite bacteria agent capable medium pH 6.0 ~ 8.0, inoculum concentration is 4%, adds sweet potato starch and oyster shell whiting, cultivate 6 days at 34 DEG C, make bacterium number in zymotic fluid reach 15 × 10
8individual/mL, namely obtains described composite feed additive.
The preparation method of the composite feed additive that the present embodiment provides, described composite bacteria agent capable culture medium prescription is: honey 1 ~ 5g/100mL, sucrose 5 ~ 10g/100mL, MC peptone 1 ~ 5g/100mL, agar 0.1 ~ 0.8g/100mL, KH
2pO
40.2 ~ 0.5g/100mL, K
2hPO
40.5 ~ 3g/100mL, MgSO
47H
2o0.5 ~ 3g/100mL, NaCl0.5 ~ 10g/100mL, all the other are distilled water.
Embodiment 2
Composite feed additive, adopts streptococcus fecalis 15%, bacillus subtilis 20%, Lactobacillus plantarum 18%, Lactobacillus casei 22%, sweet potato starch 12%, oyster shell whiting 13%, by method preparation described in embodiment 1, namely obtains described composite feed additive.
Embodiment 3
Composite feed additive, adopts streptococcus fecalis 24%, bacillus subtilis 18%, Lactobacillus plantarum 18%, Lactobacillus casei 20%, sweet potato starch 12%, oyster shell whiting 8%, by method preparation described in embodiment 1, namely obtains described composite feed additive.
Through verification experimental verification, domestic animals breeding feed additive provided by the invention, can promote the growth of the domestic animals such as pig, ox, sheep, improves survival rate, improves the output of the meat such as pig, ox, sheep simultaneously.
Claims (6)
1. composite feed additive, it is characterized in that: adopt streptococcus fecalis (Streptococcus faecalis), bacillus subtilis (Bacillus subtilis), Lactobacillus plantarum (Lactobacillus plantarum) and Lactobacillus casei (Lactobacillus casei) to prepare bacterial classification, then mix with sweet potato starch and oyster shell whiting.
2. composite feed additive according to claim 1, is characterized in that: the mass percent of described additive is: streptococcus fecalis 10 ~ 30%, bacillus subtilis 15 ~ 25%, Lactobacillus plantarum 10 ~ 25%, Lactobacillus casei 15 ~ 30%, sweet potato starch 10 ~ 15%, oyster shell whiting surplus.
3. composite feed additive according to claim 2, is characterized in that: the mass percent of described additive is: streptococcus fecalis 15 ~ 25%, bacillus subtilis 15 ~ 20%, Lactobacillus plantarum 15 ~ 20%, Lactobacillus casei 17 ~ 25%, sweet potato starch 10 ~ 12%, oyster shell whiting surplus.
4. composite feed additive according to claim 3, is characterized in that: the mass percent of described additive is: streptococcus fecalis 24%, bacillus subtilis 18%, Lactobacillus plantarum 18%, Lactobacillus casei 20%, sweet potato starch 12%, oyster shell whiting 8%.
5. the preparation method of composite feed additive according to claim 1, is characterized in that:
1. inoculate: be connected on slant medium by described each bacterial classification, cultivation temperature 30 ~ 35 DEG C, incubation time is 36 ~ 48h;
2. Spawn incubation: the culture medium picking list bacterium colony grown is forwarded to and is equipped with in the 50mL conical flask of fluid nutrient medium,
Sterile working, 34 DEG C, 130r/min cultivates 36 ~ 48h;
3. one grade fermemtation: strain inoculation will be cultivated in the 500mL conical flask filling fluid nutrient medium, sterile working, 34 DEG C, 130r/min cultivates 36 ~ 48h;
4. mixed culture fermentation: each bacterial classification bacterium liquid is inoculated in the fermentation tank filling composite bacteria agent capable culture medium in proportion, composite bacteria agent capable medium pH 6.0 ~ 8.0, inoculum concentration is 1 ~ 5%, adds sweet potato starch and oyster shell whiting, cultivate 4 ~ 7 days at 34 DEG C, make bacterium number in zymotic fluid reach 10 ~ 15 × 10
8individual/mL, namely obtains described composite feed additive.
6. the preparation method of composite feed additive according to claim 5, is characterized in that: described composite bacteria agent capable culture medium prescription is: honey 1 ~ 5g/100mL, sucrose 5 ~ 10g/100mL, MC peptone 1 ~ 5g/100mL, agar 0.1 ~ 0.8g/100mL, KH
2pO
40.2 ~ 0.5g/100mL, K
2hPO
40.5 ~ 3g/100mL, MgSO
47H
2o0.5 ~ 3g/100mL, NaCl0.5 ~ 10g/100mL, all the other are distilled water.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310637934.1A CN104664075A (en) | 2013-11-29 | 2013-11-29 | Composite feed additive |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310637934.1A CN104664075A (en) | 2013-11-29 | 2013-11-29 | Composite feed additive |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN104664075A true CN104664075A (en) | 2015-06-03 |
Family
ID=53300080
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201310637934.1A Pending CN104664075A (en) | 2013-11-29 | 2013-11-29 | Composite feed additive |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105961850A (en) * | 2016-03-10 | 2016-09-28 | 昆明理工大学 | A fermentation accelerant for feed prepared by utilizing air-dried bagasse |
-
2013
- 2013-11-29 CN CN201310637934.1A patent/CN104664075A/en active Pending
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105961850A (en) * | 2016-03-10 | 2016-09-28 | 昆明理工大学 | A fermentation accelerant for feed prepared by utilizing air-dried bagasse |
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Application publication date: 20150603 |