CN104664061A - Feed additive for increasing animal productivity - Google Patents
Feed additive for increasing animal productivity Download PDFInfo
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- CN104664061A CN104664061A CN201310637935.6A CN201310637935A CN104664061A CN 104664061 A CN104664061 A CN 104664061A CN 201310637935 A CN201310637935 A CN 201310637935A CN 104664061 A CN104664061 A CN 104664061A
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- production capacity
- streptococcus
- feed addictive
- animal production
- kaolin
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Abstract
The invention provides a feed additive for increasing animal productivity. The feed additive is obtained by preparing strains by adopting streptococcus faecalis, pediococcus acidilacticii, streptococcus lactis and lactobacillus casei and then mixing with sweet potato starch and kaolin. The feed additive is prepared from the following components in percentage by weight: 10-20% of streptococcus faecalis, 20-50% of pediococcus acidilacticii, 10-25% of streptococcus lactis, 5-15% of lactobacillus casei, 5-15% of sweet potato starch, and the rest of kaolin.
Description
Technical field
The present invention relates to a kind of feed addictive for improving animal production capacity and preparation method thereof, belonging to the technical field of biological materials that microbial modulator is applied in livestock breed aquatics.
Background technology
Along with the increase with livestock rearing density that further develops of herding intensive farm, the generation of various herding disease is more and more frequent, and the herding of most cultivation is all in sub-health state.Once widely used medicine not only made pathogenic microorganism create the serious resistance to the action of a drug, also caused the serious problems of livestock products medicament residue, and development goes down to affect the health of the mankind.Along with people are to the pay attention to day by day of animal food safety, feed also becomes the focus that everybody pays close attention to, antibiotic is as the extensive use of feed addictive, cause serious potential safety hazard, as the generation of multiple anti-medicine bacterium, the formation of superbacteria, animal food medicine exceed standard and directly threaten human health.
Microorganism formulation arises at the historic moment under this historical background, and the sustainable development for animal husbandry provides effective, a reliable approach.Adopt biotechnology composite fermentation, utilize multiple-microorganism to interact, form the system of an ecological balance.The initial definition of feed addictive is feed supplement, to add the deficiency that the composition such as vitamin and inorganic salts fills up feed Middle nutrition composition, the nutrition of balanced feed, and obtains good feeding effect.But, generally use single microorganism bacterial classification due to existing microbiological feed and additive for microbe feedstuff or mixed after multiple-microorganism is made dry powder, microorganism is wherein in inactivation or resting state, again owing to causing bacterial classification dead in manufacturing process, as feed addictive, weak effect in, fecal deodorizing long, disease-resistant promotion fowl, dirty swine, and purposes is single, microorganism plays a role condition harshness.Existingly microorganism is made the decomposite method of dry powder, not only cause the death of microorganism, and complex process, the effect of multiple-microorganism is difficult to play.
Summary of the invention
The object of the present invention is to provide a kind of feed addictive for improving animal production capacity and preparation method thereof.The present invention makes full use of the synergistic function between bacterial classification, and additive is prepared into zymotic fluid, and play and promote that domestic animals are to food conversion, nutritional labeling absorptivity, thus promote growth of animal, prevention Animal diseases, reach the object of synergy volume increase.
For achieving the above object, the present invention adopts following technical scheme:
Improve the feed addictive of animal production capacity, adopt streptococcus fecalis (Streptococcus faecalis), Pediococcus acidilactici (Pediococcus acidilacticii), streptococcus lactis (Streptococcus lactis) and Lactobacillus casei (Lactobacillus casei) to prepare bacterial classification, then mix with sweet potato starch and kaolin.
The feed addictive of raising animal production capacity provided by the invention, the mass percent of described additive is: streptococcus fecalis 10 ~ 20%, Pediococcus acidilactici 20 ~ 50%, streptococcus lactis 10 ~ 25%, Lactobacillus casei 5 ~ 15%, sweet potato starch 5 ~ 15%, kaolin surplus.
The feed addictive of raising animal production capacity provided by the invention, preferably, the mass percent of described additive is: streptococcus fecalis 13 ~ 18%, Pediococcus acidilactici 30 ~ 45%, streptococcus lactis 14 ~ 20%, Lactobacillus casei 8 ~ 12%, sweet potato starch 8 ~ 10%, kaolin 6 ~ 9%.
The feed addictive of raising animal production capacity provided by the invention, further preferably, the mass percent of described additive is: streptococcus fecalis 12%, Pediococcus acidilactici 43%, streptococcus lactis 16%, Lactobacillus casei 12%, sweet potato starch 8%, kaolin 9%.
Present invention also offers the preparation method of the feed addictive of above-mentioned raising animal production capacity, comprise the following steps:
1. inoculate: be connected on slant medium by described each bacterial classification, cultivation temperature 30 ~ 33 DEG C, incubation time is 12 ~ 36h;
2. Spawn incubation: the culture medium picking list bacterium colony grown is forwarded to and is equipped with in the 50mL conical flask of fluid nutrient medium,
Sterile working, 32 DEG C, 130r/min cultivates 12 ~ 36h;
3. one grade fermemtation: strain inoculation will be cultivated in the 500mL conical flask filling fluid nutrient medium, sterile working, 32 DEG C, 130r/min cultivates 36 ~ 72h;
4. mixed culture fermentation: each bacterial classification bacterium liquid is inoculated in the fermentation tank filling composite bacteria agent capable culture medium in proportion, composite bacteria agent capable medium pH 6.0 ~ 8.0, inoculum concentration is 1 ~ 5%, adds sweet potato starch and kaolin, cultivate 4 ~ 7 days at 32 DEG C, make bacterium number in zymotic fluid reach 10 ~ 12 × 10
8individual/mL, namely obtains the feed addictive of described raising animal production capacity.
The preparation method of the feed addictive of raising animal production capacity provided by the invention, described composite bacteria agent capable culture medium prescription is: glucose 1 ~ 5g/100mL, honey 5 ~ 10g/100mL, peptone 1 ~ 5g/100mL, beef extract 0.1 ~ 0.8g/100mL, KH
2pO
40.2 ~ 0.5g/100mL, K
2hPO
40.5 ~ 3g/100mL, MgSO
47H
2o0.5 ~ 3g/100mL, NaCl0.5 ~ 10g/100mL, all the other are distilled water.
Compared to prior art, composite microbial feed additive provided by the invention adopts streptococcus fecalis, Pediococcus acidilactici, streptococcus lactis, Lactobacillus casei, sweet potato starch and kaolin mixed culture fermentation to be prepared from.Above bacterial classification is respectively through Spawn incubation, and two-stage fermentation, makes bacterium number in zymotic fluid reach 10 ~ 12 × 10
8individual/mL, must improve the feed addictive of animal production capacity.This product is through verifying on the spot for a long time, and rationally, stable effect, can promote growth of animal to formula, improves animal product output, and improve survival rate, disease resistance, improves food conversion ratio, and improves product quality.
Detailed description of the invention
The following examples can make the present invention of those of ordinary skill in the art's comprehend, but do not limit the present invention in any way.
Embodiment 1
Improve the feed addictive of animal production capacity, adopt streptococcus fecalis 17%, Pediococcus acidilactici 43%, streptococcus lactis 22%, Lactobacillus casei 6%, sweet potato starch 9%, kaolin 3%, prepare according to the following steps:
1. inoculate: be connected on slant medium by described each bacterial classification, cultivation temperature 30 ~ 33 DEG C, incubation time is 18h;
2. Spawn incubation: the culture medium picking list bacterium colony grown is forwarded to and is equipped with in the 50mL conical flask of fluid nutrient medium,
Sterile working, 32 DEG C, 130r/min cultivates 18h;
3. one grade fermemtation: strain inoculation will be cultivated in the 500mL conical flask filling fluid nutrient medium, sterile working, 32 DEG C, 130r/min cultivates 48h;
4. mixed culture fermentation: each bacterial classification bacterium liquid is inoculated in the fermentation tank filling composite bacteria agent capable culture medium in proportion, composite bacteria agent capable medium pH 6.0 ~ 8.0, inoculum concentration is 3%, adds sweet potato starch and kaolin, cultivate 6 days at 32 DEG C, make bacterium number in zymotic fluid reach 12 × 10
8individual/mL, namely obtains the feed addictive of described raising animal production capacity.
The preparation method of the feed addictive of the raising animal production capacity that the present embodiment provides, described composite bacteria agent capable culture medium prescription is: glucose 1 ~ 5g/100mL, honey 5 ~ 10g/100mL, peptone 1 ~ 5g/100mL, beef extract 0.1 ~ 0.8g/100mL, KH
2pO
40.2 ~ 0.5g/100mL, K
2hPO
40.5 ~ 3g/100mL, MgSO
47H
2o0.5 ~ 3g/100mL, NaCl0.5 ~ 10g/100mL, all the other are distilled water.
Embodiment 2
Improve the feed addictive of animal production capacity, adopt streptococcus fecalis 13%, Pediococcus acidilactici 40%, streptococcus lactis 17%, Lactobacillus casei 11%, sweet potato starch 10%, kaolin 9%, by method preparation described in embodiment 1, namely obtain described composite feed additive.
Embodiment 3
Improve the feed addictive of animal production capacity, adopt streptococcus fecalis 12%, Pediococcus acidilactici 43%, streptococcus lactis 16%, Lactobacillus casei 12%, sweet potato starch 8%, kaolin 9%, by method preparation described in embodiment 1, namely obtain described composite feed additive.
Through verification experimental verification, domestic animals breeding feed additive provided by the invention, can promote the growth of the domestic animals such as pig, ox, sheep, improves survival rate, can improve the domestic animals cultivated animals production capacities such as pig, ox, sheep significantly.
Claims (6)
1. improve the feed addictive of animal production capacity, it is characterized in that: adopt streptococcus fecalis (Streptococcus faecalis), Pediococcus acidilactici (Pediococcus acidilacticii), streptococcus lactis (Streptococcus lactis) and Lactobacillus casei (Lactobacillus casei) to prepare bacterial classification, then mix with sweet potato starch and kaolin.
2. the feed addictive of raising animal production capacity according to claim 1, is characterized in that: the mass percent of described additive is: streptococcus fecalis 10 ~ 20%, Pediococcus acidilactici 20 ~ 50%, streptococcus lactis 10 ~ 25%, Lactobacillus casei 5 ~ 15%, sweet potato starch 5 ~ 15%, kaolin surplus.
3. the feed addictive of raising animal production capacity according to claim 2, is characterized in that: the mass percent of described additive is: streptococcus fecalis 13 ~ 18%, Pediococcus acidilactici 30 ~ 45%, streptococcus lactis 14 ~ 20%, Lactobacillus casei 8 ~ 12%, sweet potato starch 8 ~ 10%, kaolin 6 ~ 9%.
4. the feed addictive of raising animal production capacity according to claim 3, is characterized in that: the mass percent of described additive is: streptococcus fecalis 12%, Pediococcus acidilactici 43%, streptococcus lactis 16%, Lactobacillus casei 12%, sweet potato starch 8%, kaolin 9%.
5. the preparation method of the feed addictive of raising animal production capacity according to claim 1, is characterized in that:
1. inoculate: be connected on slant medium by described each bacterial classification, cultivation temperature 30 ~ 33 DEG C, incubation time is 12 ~ 36h;
2. Spawn incubation: the culture medium picking list bacterium colony grown is forwarded to and is equipped with in the 50mL conical flask of fluid nutrient medium, sterile working, 32 DEG C, 130r/min cultivates 12 ~ 36h;
3. one grade fermemtation: strain inoculation will be cultivated in the 500mL conical flask filling fluid nutrient medium, sterile working, 32 DEG C, 130r/min cultivates 36 ~ 72h;
4. mixed culture fermentation: each bacterial classification bacterium liquid is inoculated in the fermentation tank filling composite bacteria agent capable culture medium in proportion, composite bacteria agent capable medium pH 6.0 ~ 8.0, inoculum concentration is 1 ~ 5%, adds sweet potato starch and kaolin, cultivate 4 ~ 7 days at 32 DEG C, make bacterium number in zymotic fluid reach 10 ~ 12 × 10
8individual/mL, namely obtains the feed addictive of described raising animal production capacity.
6. the preparation method of the feed addictive of raising animal production capacity according to claim 5, is characterized in that: described composite bacteria agent capable culture medium prescription is: glucose 1 ~ 5g/100mL, honey 5 ~ 10g/100mL, peptone 1 ~ 5g/100mL, beef extract 0.1 ~ 0.8g/100mL, KH
2pO
40.2 ~ 0.5g/100mL, K
2hPO
40.5 ~ 3g/100mL, MgSO
47H
2o0.5 ~ 3g/100mL, NaCl0.5 ~ 10g/100mL, all the other are distilled water.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310637935.6A CN104664061A (en) | 2013-11-29 | 2013-11-29 | Feed additive for increasing animal productivity |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310637935.6A CN104664061A (en) | 2013-11-29 | 2013-11-29 | Feed additive for increasing animal productivity |
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| Publication Number | Publication Date |
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| CN104664061A true CN104664061A (en) | 2015-06-03 |
Family
ID=53300066
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112493366A (en) * | 2020-12-07 | 2021-03-16 | 北京市农林科学院 | Compound microbial inoculum for solid-state fermentation weaned piglet feed, preparation method and application |
-
2013
- 2013-11-29 CN CN201310637935.6A patent/CN104664061A/en active Pending
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112493366A (en) * | 2020-12-07 | 2021-03-16 | 北京市农林科学院 | Compound microbial inoculum for solid-state fermentation weaned piglet feed, preparation method and application |
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Application publication date: 20150603 |