CN104623541A - Traditional Chinese medicine conductive to enhancing immunity and preparation method thereof - Google Patents
Traditional Chinese medicine conductive to enhancing immunity and preparation method thereof Download PDFInfo
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Abstract
The invention discloses a traditional Chinese medicine conductive to enhancing immunity. An effective ingredient of the traditional Chinese medicine consists of the following components in parts by weight: 65-75 parts of a radix curcumae longae extract, 42-45 parts of a lucid ganoderma extract, 15-18 parts of a dendrobe extract, 36-38 parts of a polygonum cuspidatum extract, 11-14 parts of a cortex magnolia officinalis extract, 2-7 parts of an aloe extract and 19-22 parts of an eucommia ulmoide extract. Each extract is extracted under specific technological parameters such as solvent, temperature and time. Finally, the extracts are uniformly mixed to prepare a corresponding preparation. The traditional Chinese medicine is excellent in capability of enhancing the immunity and also commendably has the functions of resisting thrombus and regulating the cardiovascular system.
Description
Technical field
The present invention relates to Chinese drug preparation field, particularly a kind of Chinese medicine contributing to enhancing immunity and preparation method thereof.
Background technology
Immunity generally also can be resistance.The body immunity of usual theory be exactly health when being subject to external infringement, such as when antibacterial, poisoning intrusion human body, the ability of body resistance exotic invasive.The immunity of human body comes from immune system, immune system mainly contains the function of three aspects, and first is immune defence, it to antigenic substance as: pathogenic microorganism, vaccine, toxoid, foreign protein etc. occur immunne response, final elimination antigenic substance, plays the effect of infection immunity.If during the defense function generation defect of body, very easily suffer from infectiousness or infectious disease; Second is immunological homeostasis, can remove damage, aging, dead cell, and effectively carry out immunoregulation effect, as from steady functional disorder, easily occur autoimmune disease; It three is immune surveillance functions, its major function shows that body is by the antigen on immunocyte identification cancerous tumor cell, and by special or non-specific killer cell by cancer cell destruction, namely emerging cancerous cell is eliminated before not causing a large amount of propagation, diffusion, and plays antineoplastic action.As immune surveillance mistake department, often easily there is tumor.Along with the progress of society, the quickening of people's rhythm of life, the change of living environment, and the increasing pressure of each side is large, causes the crowd of immunity degradation also to get more and more thus.So enhancing human body immunity power, safeguard healthy most important.
The power of immunity derives from strong immune system, and immune system comprises immune organ, immunocyte and immune molecule.Immunocyte mainly comprises T and bone-marrow-derived lymphocyte, monokaryon or macrophage, dendritic cell, acidophilia and basophilia polymorphonuclear cell, erythrocyte and platelet, and antibody secreted by these cells and cytokine; Immune organ comprises liver, spleen, lymph node, thymus, bone marrow etc.Therefore, only the function of guaranteed each immune system organs, tissue, cell is normal, could improve body immunity, keep fit.
CN 102000122 A discloses a kind of pharmaceutical composition with raising immunity, effective ingredient is by fermented Cordyceps powder or Cordyceps polysaccharide, vitamin, mineral form, wherein fermented Cordyceps powder 0.1-2 weight portion or Cordyceps polysaccharide 0.5-3 weight portion, vitamin is 0.001-0.003 weight portion, mineral calcium, phosphorus, magnesium, sodium, potassium one or more be 0.1-1 weight portion and/or mineral ferrum, zinc, manganese, copper, chromium, selenium one or more be 0.01-0.03 weight portion.In said composition, the raw material Cordyceps of fermented Cordyceps powder is expensive, and need fermentation technology to prepare in addition, the time is tediously long, and vitamin and mineral are outer doping, adds the cost of preparation, and neither be ideal to the regulating effect of immunity.In addition, in the Chinese medicine composition of all existing enhancing immunity described above, function ratio is more single, often can only play this kind of effect of enhancing immunity, cannot have other effects concurrently, even if it is also undesirable to possess effect.
Summary of the invention
For above-mentioned the deficiencies in the prior art, the invention provides a kind of Chinese medicine contributing to enhancing immunity and preparation method thereof, this Chinese medicine has the ability of excellent enhancing immunity, and can be good have antithrombotic concurrently, regulate the function of cardiovascular system.
A kind of Chinese medicine contributing to enhancing immunity provided by the invention, its effective ingredient is made up of the component of following weight portion: Rhizoma Curcumae Longae extract 65-75 part, Ganoderma extract 42-45 part, Herba Dendrobii extract 15-18 part, Rhizoma Polygoni Cuspidati extract 36-38 part, Cortex Magnoliae Officinalis extract 11-14 part, Aloe extract 2-7 part, the Cortex Eucommiae extracts 19-22 part;
Described Rhizoma Curcumae Longae extract prepares by the following method: get Rhizoma Curcumae Longae raw material, clean and dry rear pulverizing, add 75% alcohol reflux 3 times, extraction time first time is 120min, and extraction time is 80min for the second time, for the third time, each Extracting temperature is 65 DEG C, during each extraction, the rate of charge of Rhizoma Curcumae Longae raw material and ethanol is: every 15kg Rhizoma Curcumae Longae raw material adds 75% ethanol of 140L, has extracted rear filtration three times, then merge extractive liquid, dry after concentrating under reduced pressure, obtain Rhizoma Curcumae Longae extract;
Described Ganoderma extract prepares by the following method: get Ganoderma raw material, clean the Ganoderma tablet being cut into 1-3mm after drying, Ganoderma tablet to be added in the ethanol of 85% reflux, extract, 3 times, extraction time first time is 110min, second time extraction time is 90min, extraction time third time is 70min, and Extracting temperature is 60 DEG C, and during each extraction, the rate of charge of Ganoderma raw material and ethanol is: 85% ethanol adding 115L in every 15kg Ganoderma raw material; Extract rear filtration three times, then merge extractive liquid, dry after concentrating under reduced pressure, obtain Ganoderma extract;
Described Herba Dendrobii extract prepares by the following method: get Herba Dendrobii raw material, clean and dry rear pulverizing, be added to the water decoction 4 times, decocting time is 70min for the first time, second time and for the third time decocting time are 60min, and the 4th time decocting time is 45min, and the weight at every turn added water is 6-7 times of Herba Dendrobii raw material weight, decoction liquor filtered after decoction and merge, at 45-50 DEG C, concentrating under reduced pressure, drying obtain Herba Dendrobii extract;
Described Rhizoma Polygoni Cuspidati extract prepares by the following method: get Rhizoma Polygoni Cuspidati raw material, clean after drying and add 65% alcohol reflux 2 times, extraction time first time is 90min, second time extraction time is 60min, and each Extracting temperature is 45 DEG C, and during each extraction, the rate of charge of Rhizoma Polygoni Cuspidati raw material and ethanol is: every 20kg Rhizoma Polygoni Cuspidati raw material adds 65% ethanol of 160L, filtration after extracted twice completes, then merge extractive liquid, dry after concentrating under reduced pressure, obtain Rhizoma Polygoni Cuspidati extract;
Described Cortex Magnoliae Officinalis extract prepares by the following method: get magnolia bark raw material, clean after drying and be added to the water decoction 3 times, decocting time is 160min for the first time, second time decocting time is 100min, decocting time is 45min for the third time, the weight that each decoction adds water is 9-10 times of magnolia bark raw material, and at 45-50 DEG C, concentrating under reduced pressure, drying obtain Cortex Magnoliae Officinalis extract;
Described Aloe extract prepares by the following method: get aloe raw material, smashes after cleaning, and adopt super-critical fluid extraction to extract, Extraction solvent is carbon dioxide, and Extracting temperature is 55 DEG C, and pressure is 16-18MPa, obtains Aloe extract after extracting 40min;
Described Cortex Eucommiae extract prepares by the following method: get Cortex Eucommiae raw material, clean after drying and add 95% alcohol reflux 3 times, extraction time first time is 70min, extraction time is 55min for the second time, for the third time, and each Extracting temperature is 60 DEG C, and during each extraction, the rate of charge of Cortex Eucommiae raw material and ethanol is: every 15kg Cortex Eucommiae adds 95% ethanol of 95L, extract rear filtration three times, then merge extractive liquid, dry after concentrating under reduced pressure, obtain Cortex Eucommiae extract.
Preferably, described middle the effective elements of the medicine is made up of the component of following weight portion: Rhizoma Curcumae Longae extract 72 parts, Ganoderma extract 43 parts, Herba Dendrobii extract 17 parts, Rhizoma Polygoni Cuspidati extract 36 parts, Cortex Magnoliae Officinalis extract 12 parts, Aloe extract 4 parts, and the Cortex Eucommiae extracts 20 parts;
Preferably, the relative density of extracting solution after concentrating under reduced pressure is 1.20 ~ 1.23.
The above-mentioned preparation method contributing to the Chinese medicine of enhancing immunity, in turn includes the following steps:
(1) extract preparation:
Get Rhizoma Curcumae Longae raw material, clean and dry rear pulverizing, add 75% alcohol reflux 3 times, extraction time first time is 120min, and extraction time is 80min for the second time, for the third time, each Extracting temperature is 65 DEG C, during each extraction, the rate of charge of Rhizoma Curcumae Longae raw material and ethanol is: every 15kg Rhizoma Curcumae Longae raw material adds 75% ethanol of 140L, has extracted rear filtration three times, then merge extractive liquid, dry after concentrating under reduced pressure, obtain Rhizoma Curcumae Longae extract;
Get Ganoderma raw material, clean the Ganoderma tablet being cut into 1-3mm after drying, Ganoderma tablet to be added in the ethanol of 85% reflux, extract, 3 times, extraction time first time is 110min, second time extraction time is 90min, extraction time third time is 70min, and Extracting temperature is 60 DEG C, and during each extraction, the rate of charge of Ganoderma raw material and ethanol is: 85% ethanol adding 115L in every 15kg Ganoderma raw material; Extract rear filtration three times, then merge extractive liquid, dry after concentrating under reduced pressure, obtain Ganoderma extract;
Get Herba Dendrobii raw material, clean and dry rear pulverizing, be added to the water decoction 4 times, decocting time is 70min for the first time, second time and for the third time decocting time are 60min, and the 4th time decocting time is 45min, and the weight at every turn added water is 6-7 times of Herba Dendrobii raw material weight, decoction liquor filtered after decoction and merge, at 45-50 DEG C, concentrating under reduced pressure, drying obtain Herba Dendrobii extract;
Get Rhizoma Polygoni Cuspidati raw material, clean after drying and add 65% alcohol reflux 2 times, extraction time first time is 90min, second time extraction time is 60min, and each Extracting temperature is 45 DEG C, and during each extraction, the rate of charge of Rhizoma Polygoni Cuspidati raw material and ethanol is: every 20kg Rhizoma Polygoni Cuspidati raw material adds 65% ethanol of 160L, filtration after extracted twice completes, then merge extractive liquid, dry after concentrating under reduced pressure, obtain Rhizoma Polygoni Cuspidati extract;
Get magnolia bark raw material, clean after drying and be added to the water decoction 3 times, decocting time is 160min for the first time, second time decocting time is 100min, decocting time is 45min for the third time, the weight that each decoction adds water is 9-10 times of magnolia bark raw material, and at 45-50 DEG C, concentrating under reduced pressure, drying obtain Cortex Magnoliae Officinalis extract;
Get aloe raw material, smash after cleaning, adopt super-critical fluid extraction to extract, Extraction solvent is carbon dioxide, and Extracting temperature is 55 DEG C, and pressure is 16-18MPa, obtains Aloe extract after extracting 40min;
Get Cortex Eucommiae raw material, clean after drying and add 95% alcohol reflux 3 times, extraction time first time is 70min, extraction time is 55min for the second time, for the third time, and each Extracting temperature is 60 DEG C, and during each extraction, the rate of charge of Cortex Eucommiae raw material and ethanol is: every 15kg Cortex Eucommiae adds 95% ethanol of 95L, extract rear filtration three times, then merge extractive liquid, dry after concentrating under reduced pressure, obtain Cortex Eucommiae extract;
(2) the extract mix homogeneously of following weight portion is got: Rhizoma Curcumae Longae extract 65-75 part, Ganoderma extract 42-45 part, Herba Dendrobii extract 15-18 part, Rhizoma Polygoni Cuspidati extract 36-38 part, Cortex Magnoliae Officinalis extract 11-14 part, Aloe extract 2-7 part, the Cortex Eucommiae extracts 19-22 part;
(3) mixed extract is made pharmaceutically acceptable oral formulations.
Described oral formulations comprises capsule, granule, electuary, tablet and pill.Above-mentioned preparation can add the acceptable excipient of pharmacy and obtain in the extract of effective dose, is convenient to storage and preservation after making.
Rhizoma Curcumae Longae in the present invention, Herba Dendrobii, Rhizoma Polygoni Cuspidati, Cortex Magnoliae Officinalis, Aloe, the Cortex Eucommiae all directly can be buied by Chinese medicinal material market, wherein:
Rhizoma Curcumae Longae: be the rhizome of zingiberaceous plant Rhizoma Curcumae Longae or Radix Curcumae, removing blood stasis, circulation of qi promoting, stimulates the menstrual flow, pain relieving.Control trusted subordinate's feeling of fullness distending pain, brachialgia ， mass in the abdomen, women's blood stasis amenorrhea, puerperal, the stasis of blood stopped stomachache, injury from falling down, carbuncle.Breast for qi depression to blood stasis is suffered from abdominal pain, dysmenorrhea and limbs pain, often joins Rhizoma Corydalis, Rhizoma Cyperi.Curcumol in Rhizoma Curcumae Longae or ether extract, curcumin and volatile oil gavage, experimental atherosclerosis rats and rabbit are had to the effect of significantly falling total plasma cholesterol and B-lipoprotein, and can liver cholesterol be reduced, correct a-and B-lipoprotein out of proportion, but on endogenous cholesterol without impact; The effect of falling plasma triglyceride is more shown, triglyceride in blood plasma can be made to be reduced to below normal level.Effect of high sucrose food can cause rat to produce hyperlipemia, and curcumin can resist this hyperlipemia and produce.It is heavy that gavage curcumin can reduce liver, reduce the total triglyceride of triglyceride, free fatty, content of phospholipid and serum, VLDL+LDL triglyceride in liver, the content of HDL triglyceride, VLDL+LDL cholesterol and blood free fatty acid, also can improve serum total cholesterol and HDL cholesterol level.With the external thermal-insulating method of liver homogenate, with 14C-acetic acid for Substrate Assay, PRELIMINARY RESULTS shows, curcumin can suppress the synthesis of fatty acid.
Ganoderma: property is flat, sweet in the mouth, GUIXIN, lung, liver, kidney channel.Compendium of Material Medica is recorded, and Ganoderma can " be controlled and tie in the heart, the motive of hanging ", " enter the heart being responsible for production of blood, help the heart to fill arteries and veins ", " calming the nerves ", and " protecting god ", " lung benefiting, spleen, vital essence ", " tonifying liver gas " etc., all have benefiting action to whole body QI of five ZANG-organs.
Herba Dendrobii: Shennong's Herbal records Herba Dendrobii " main damaging the spleen and stomach, eliminating impediment, the therapeutic method to keep the adverse QI flowing downwards, tonifying five ZANG-organs asthenia weakness and emaciation, reinforcing YIN-essence, take thick the intestines and stomach for a long time "; Herba Dendrobii " reinforcing YIN-essence benefit is smart, thick the intestines and stomach, never sufficient in benefit, flat gastric qi, longue meat, and Fructus Alpiniae Oxyphyllae, except frightened, made light of one's life by commiting suicide and prolonged life " is evaluated in Compendium of Material Medica; The stem of Herba Dendrobii can clearing away heat and promoting production of body fluid, and anti-inflammatory analgetic, clear and rich throat, has good curative effect to treatment hoarse voice.Herba Dendrobii has good resisting fatigue, the effect of anoxia enduring.
Cortex Magnoliae Officinalis: acrid in the mouth, warm in nature, has effect of circulation of qi promoting removing dampness, warming middle-JIAO to relieve pain, lowering the adverse QI to relieve asthma.Cortex Magnoliae Officinalis decoct has stronger antibacterial action to staphylococcus, streptococcus, bloody dysentery bacillus, pasteurellosis bacillus, vibrio cholera; And also have certain mitigation to striped muscle is tetanic.Bark, root bark, flower, seed and bud all can be used as medicine, based on bark, be famous Chinese medicine, have removing dampness intestinal stasis relieving, circulation of qi promoting is relievingd asthma, relieving dyspepsia expectorant, wind dispelling analgesia effect; Seed has improving eyesight QI invigorating effect, and bud is used as gynecological drug.
Rhizoma Polygoni Cuspidati: sweetness and bitterness is pungent, temperature, returns liver, gallbladder, lung meridian, promoting blood circulation to remove blood stasis; Dispelling wind and removing obstruction in the collateral; Clearing away heat-damp and promoting diuresis; Removing toxic substances.Housewife female's amenorrhea; Dysmenorrhea; Lochiostasis; Lump in the abdomen; Injury from falling down; Rheumatic arthralgia; Jaundice due to damp-heat; Stranguria with turbid discharge leukorrhagia; Sore swollen toxin; Venom; Burn due to hot liquid or fire.Containing abundant resveratrol in Rhizoma Polygoni Cuspidati, the functions such as anticancer, antiinflammatory, protection cardiovascular can be played.
Aloe: can pathogenic fire purging; Removing toxic substances; Blood stasis dispelling; Parasite killing.Argument is red; Constipation; Nebulousurine; Hematuria; Pediatric epilepsy scared; Infantile malnutrition; Burn and scald; Women's amenorrhea; Hemorrhoid; Scabies; Carbuncle furuncle and phyma poison; Traumatic injury.Its Aloin A contained, wound hormone and polysaccharide peptide manna (Ke-2) etc. have viral infection resisting, promote the effect that wound healing restores, have the function of anti-inflammation and sterilization, heat absorption detumescence, softening skin, maintenance cell viability, curdlan is combined with 1-decene-1 and is also had healing wounds activity.
The Cortex Eucommiae: temperature; Sweet; Return liver, kidney channel, Shennong's Herbal record, the main pain along the spinal column of the Cortex Eucommiae, invigorating middle warmer benefit vital essence, hard muscles and bones, Qiang Zhi, except wet, dribbling urination of itching under the moon.Compendium of Material Medica is recorded: Cortex Eucommiae color is purple and moisten, and sweet in the mouth is micro-pungent, and its temperature is put down, and Gan Wenneng mends, micro-pungent energy profit, therefore can enter liver and the kidney invigorating.The iridoid glycosides separated from Cortex Eucommiae, lignanoids water solubility extract is oral hypotensive effect; Dilating effect is had to normal rabbit coronary artery and Renal vascular.In doctor trained in Western medicine theory, the kidney " yang deficiency " of the traditional Chinese medical science is the hypofunction due to pitutary-adrenal system, thus cause multiple the adverse effects such as cylinder electrolyte disorder, gastrointestinal dyspepsia, physical stamina letdown, lassitude, and use Cortex Eucommiae kidney warming and yang invigorating medicine can have certain regulating action to pituitary adrenal cortex hormone function, physical function can be synthesized and coordinated from many aspects, comprehensive reinforcement is carried out to body immunity.
A kind of Chinese medicine contributing to enhancing immunity provided by the invention, possesses following beneficial effect:
(1) theory of Chinese medical science, the immune defense function of human body belongs to the category of healthy energy, and it had both comprised the natural protection function of body, also comprised specific immune response.If QI and blood fills, healthy energy is sufficient, QI movement being in harmonious way, then how normal immunologic function is, then not easily attacked by pathogens such as pathogenic microorganisms; Weak QI and blood is deficient, positive QI-insufficiency, the many defects of immunologic function or low, then easily caused disease by pathogen invasion and attack such as pathogenic microorganisms.Therefore ancients have the theory of " healthy energy deposit in, heresy can not be done ", " gathering of heresy, its gas must be empty ", describing the healthy energy improving human body is enhancing human body immunity power, the key factor of preventing and curing diseases.Each component in the present invention plays multiple coordinative role in raising human righteousness, such as: Rhizoma Curcumae Longae extract and Ganoderma extract are that major ingredient can the gas of tonifying five ZANG-organs, healthy energy in strong body, Herba Dendrobii extract improves at Rhizoma Curcumae Longae extract can the therapeutic method to keep the adverse QI flowing downwards on the basis of healthy energy, prevent depressed, and Herba Dendrobii extract can also " flat gastric qi ", the gas pancake under one's belt that each component is mended, facilitating digestion system absorbs the effective ingredient of each component, Cortex Magnoliae Officinalis extract is in other component QI invigorating, there is while the therapeutic method to keep the adverse QI flowing downwards effect of " circulation of qi promoting ", institute's correction gas can be made current smooth and easy in human body, ensure that healthy energy can play maximum effect when immunity moderation system, Cortex Eucommiae extract leniently can supplement kidney qi, and by kidney channel, the multiple blood circulation of human body is played to the effect planning as a whole to be in harmonious proportion, Rhizoma Polygoni Cuspidati extract and Aloe extract have antiinflammatory, sterilization, the effects such as promoting blood circulation to remove obstruction in the collateral, under the epidermis that these effects are mainly reflected in human body and epidermis, with other component cooperation, from outside to inside omnibearing protection is carried out to the immune system of human body.
(2) according to doctor trained in Western medicine zoopery, the present invention is by the strict control to the solvent of extraction process, temperature, time and each component proportion, make component in extract can not only adjusting immunity of human body, can also play regulating action to cardiovascular system, obtained medicament has composite efficacy.
(3) preparation method is simple, does not need too much additive, does not also need complicated fermentation, enzymolysis process, the material benefit of needed raw material price.
Detailed description of the invention
In order to make those skilled in the art person understand technical scheme of the present invention better, below in conjunction with detailed description of the invention, the present invention is described in further detail.
Embodiment 1
Preparation contributes to the Chinese medicine of enhancing immunity in accordance with the following steps:
(1) extract preparation:
Get 15kg Rhizoma Curcumae Longae raw material, clean and dry rear pulverizing, add the alcohol reflux 3 times that volumetric concentration is 75%, extraction time first time is 120min, for the second time, extraction time third time is 80min, each Extracting temperature is 65 DEG C, each extraction adds ethanol 140L, extracted rear filtration three times, then merge extractive liquid, concentrating under reduced pressure becomes the concentrated solution of relative density 1.20, dry with spray drying method, the inlet temperature of spray-drying process is 160 DEG C, and leaving air temp is 80 DEG C, obtains Rhizoma Curcumae Longae extract;
Get 15kg Ganoderma raw material, clean the Ganoderma tablet being cut into 1mm after drying, Ganoderma tablet to be added in the ethanol of 85% reflux, extract, 3 times, extraction time first time is 110min, second time extraction time is 90min, extraction time third time is 70min, and Extracting temperature is 60 DEG C, adds 85% ethanol of 115L during each extraction; Extracted rear filtration three times, then merge extractive liquid, concentrating under reduced pressure becomes the concentrated solution of relative density 1.20, and rear spray drying method is dry, and the inlet temperature of spray-drying process is 160 DEG C, and air outlet temperature is 80 DEG C, obtains Ganoderma extract;
Get Herba Dendrobii raw material 15kg, clean and dry rear pulverizing, be added to the water decoction 4 times, decocting time is 70min for the first time, second time and for the third time decocting time are 60min, 4th time decocting time is 45min, the weight at every turn added water is 7 times of Herba Dendrobii raw material weight, after decoction, decoction liquor is filtered and merge, at 45-50 DEG C, concentrating under reduced pressure becomes the concentrated solution of relative density 1.20, and dry with spray drying method, the inlet temperature of spray-drying process is 140 DEG C, leaving air temp is 80 DEG C, and drying obtains Herba Dendrobii extract;
Get 20kg Rhizoma Polygoni Cuspidati raw material, clean after drying and add 65% alcohol reflux 2 times, extraction time first time is 90min, second time extraction time is 60min, each Extracting temperature is 45 DEG C, and each extraction amount of alcohol added is 160L, filtration after extracted twice completes, then merge extractive liquid, concentrating under reduced pressure becomes the concentrated solution of relative density 1.22, and dry with spray drying method, the inlet temperature of spray-drying process is 160 DEG C, leaving air temp is 70 DEG C, obtains Rhizoma Polygoni Cuspidati extract;
Get 20kg magnolia bark raw material, clean after drying and be added to the water decoction 3 times, decocting time is 160min for the first time, second time decocting time is 100min, and decocting time is 45min for the third time, decocts 10 times that the weight added water is magnolia bark raw material at every turn, at 45 DEG C, concentrating under reduced pressure becomes the concentrated solution of relative density 1.20, uses spray drying method dry, the inlet temperature of spray-drying process is 160 DEG C, and leaving air temp is 80 DEG C, obtains Cortex Magnoliae Officinalis extract;
Get aloe raw material 20kg, smash after cleaning, adopt super-critical fluid extraction to extract, Extraction solvent is carbon dioxide, and Extracting temperature is 55 DEG C, and pressure is 16MPa, obtains Aloe extract after extracting 40min;
Get 15kg Cortex Eucommiae raw material, clean after drying and add 95% alcohol reflux 3 times, extraction time first time is 70min, for the second time, extraction time third time is 55min, each Extracting temperature is 60 DEG C, and each extraction adds 95% ethanol of 95L, has extracted rear filtration three times, then merge extractive liquid, concentrating under reduced pressure becomes the concentrated solution of relative density 1.21, and dry with spray drying method, the inlet temperature of spray-drying process is 140 DEG C, leaving air temp is 70 DEG C, obtains Cortex Eucommiae extract;
(2) get the extract mix homogeneously of following weight portion: Rhizoma Curcumae Longae extract 65 parts, Ganoderma extract 42 parts, Herba Dendrobii extract 18 parts, Rhizoma Polygoni Cuspidati extract 36 parts, Cortex Magnoliae Officinalis extract 14 parts, Aloe extract 2 parts, the Cortex Eucommiae extracts 22 parts.
Embodiment 2
Get 15kg Rhizoma Curcumae Longae raw material, clean and dry rear pulverizing, add the alcohol reflux 3 times that volumetric concentration is 75%, extraction time first time is 120min, for the second time, extraction time third time is 80min, each Extracting temperature is 65 DEG C, each extraction adds ethanol 140L, extracted rear filtration three times, then merge extractive liquid, concentrating under reduced pressure becomes the concentrated solution of relative density 1.20, dry with spray drying method, the inlet temperature of spray-drying process is 140 DEG C, and leaving air temp is 80 DEG C, obtains Rhizoma Curcumae Longae extract;
Get 15kg Ganoderma raw material, clean the Ganoderma tablet being cut into 3mm after drying, Ganoderma tablet to be added in the ethanol of 85% reflux, extract, 3 times, extraction time first time is 110min, second time extraction time is 90min, extraction time third time is 70min, and Extracting temperature is 60 DEG C, adds 85% ethanol of 115L during each extraction; Extracted rear filtration three times, then merge extractive liquid, concentrating under reduced pressure becomes the concentrated solution of relative density 1.20, and rear spray drying method is dry, and the inlet temperature of spray-drying process is 160 DEG C, and air outlet temperature is 80 DEG C, obtains Ganoderma extract;
Get Herba Dendrobii raw material 15kg, clean and dry rear pulverizing, be added to the water decoction 4 times, decocting time is 70min for the first time, second time and for the third time decocting time are 60min, 4th time decocting time is 45min, the weight at every turn added water is 6 times of Herba Dendrobii raw material weight, after decoction, decoction liquor is filtered and merge, at 50 DEG C, concentrating under reduced pressure becomes the concentrated solution of relative density 1.20, and dry with spray drying method, the inlet temperature of spray-drying process is 140 DEG C, leaving air temp is 80 DEG C, and drying obtains Herba Dendrobii extract;
Get 20kg Rhizoma Polygoni Cuspidati raw material, clean after drying and add 65% alcohol reflux 2 times, extraction time first time is 90min, second time extraction time is 60min, each Extracting temperature is 45 DEG C, and each extraction amount of alcohol added is 160L, filtration after extracted twice completes, then merge extractive liquid, concentrating under reduced pressure becomes the concentrated solution of relative density 1.20, and dry with spray drying method, the inlet temperature of spray-drying process is 140 DEG C, leaving air temp is 70 DEG C, obtains Rhizoma Polygoni Cuspidati extract;
Get 20kg magnolia bark raw material, clean after drying and be added to the water decoction 3 times, decocting time is 160min for the first time, second time decocting time is 100min, and decocting time is 45min for the third time, decocts 9 times that the weight added water is magnolia bark raw material at every turn, at 45-50 DEG C, concentrating under reduced pressure becomes the concentrated solution of relative density 1.20, uses spray drying method dry, the inlet temperature of spray-drying process is 140 ~ 160 DEG C, and leaving air temp is 70 ~ 80 DEG C, obtains Cortex Magnoliae Officinalis extract;
Get aloe raw material 20kg, smash after cleaning, adopt super-critical fluid extraction to extract, Extraction solvent is carbon dioxide, and Extracting temperature is 55 DEG C, and pressure is 18MPa, obtains Aloe extract after extracting 40min;
Get 15kg Cortex Eucommiae raw material, clean after drying and add 95% alcohol reflux 3 times, extraction time first time is 70min, for the second time, extraction time third time is 55min, each Extracting temperature is 60 DEG C, and each extraction adds 95% ethanol of 95L, has extracted rear filtration three times, then merge extractive liquid, concentrating under reduced pressure becomes the concentrated solution of relative density 1.20, and dry with spray drying method, the inlet temperature of spray-drying process is 160 DEG C, leaving air temp is 80 DEG C, obtains Cortex Eucommiae extract;
(2) get the extract mix homogeneously of following weight portion: Rhizoma Curcumae Longae extract 75 parts, Ganoderma extract 45 parts, Herba Dendrobii extract 15 parts, Rhizoma Polygoni Cuspidati extract 38 parts, Cortex Magnoliae Officinalis extract 11 parts, Aloe extract 7 parts, the Cortex Eucommiae extracts 19 parts.
Embodiment 3
Get 15kg Rhizoma Curcumae Longae raw material, clean and dry rear pulverizing, add the alcohol reflux 3 times that volumetric concentration is 75%, extraction time first time is 120min, for the second time, extraction time third time is 80min, each Extracting temperature is 65 DEG C, each extraction adds ethanol 140L, extracted rear filtration three times, then merge extractive liquid, concentrating under reduced pressure becomes the concentrated solution of relative density 1.23, dry with spray drying method, the inlet temperature of spray-drying process is 140 DEG C, and leaving air temp is 70 DEG C, obtains Rhizoma Curcumae Longae extract;
Get 15kg Ganoderma raw material, clean the Ganoderma tablet being cut into 2mm after drying, Ganoderma tablet to be added in the ethanol of 85% reflux, extract, 3 times, extraction time first time is 110min, second time extraction time is 90min, extraction time third time is 70min, and Extracting temperature is 60 DEG C, adds 85% ethanol of 115L during each extraction; Extracted rear filtration three times, then merge extractive liquid, concentrating under reduced pressure becomes the concentrated solution of relative density 1.20, and rear spray drying method is dry, and the inlet temperature of spray-drying process is 160 DEG C, and air outlet temperature is 80 DEG C, obtains Ganoderma extract;
Get Herba Dendrobii raw material 15kg, clean and dry rear pulverizing, be added to the water decoction 4 times, decocting time is 70min for the first time, second time and for the third time decocting time are 60min, 4th time decocting time is 45min, the weight at every turn added water is 6.5 times of Herba Dendrobii raw material weight, after decoction, decoction liquor is filtered and merge, at 47 DEG C, concentrating under reduced pressure becomes the concentrated solution of relative density 1.20, and dry with spray drying method, the inlet temperature of spray-drying process is 140 DEG C, leaving air temp is 70 DEG C, and drying obtains Herba Dendrobii extract;
Get 20kg Rhizoma Polygoni Cuspidati raw material, clean after drying and add 65% alcohol reflux 2 times, extraction time first time is 90min, second time extraction time is 60min, each Extracting temperature is 45 DEG C, and each extraction amount of alcohol added is 160L, filtration after extracted twice completes, then merge extractive liquid, concentrating under reduced pressure becomes the concentrated solution of relative density 1.20, and dry with spray drying method, the inlet temperature of spray-drying process is 160 DEG C, leaving air temp is 70 DEG C, obtains Rhizoma Polygoni Cuspidati extract;
Get 20kg magnolia bark raw material, clean after drying and be added to the water decoction 3 times, decocting time is 160min for the first time, second time decocting time is 100min, and decocting time is 45min for the third time, decocts 10 times that the weight added water is magnolia bark raw material at every turn, at 45 DEG C, concentrating under reduced pressure becomes the concentrated solution of relative density 1.20, uses spray drying method dry, the inlet temperature of spray-drying process is 140 DEG C, and leaving air temp is 70 DEG C, obtains Cortex Magnoliae Officinalis extract;
Get aloe raw material 20kg, smash after cleaning, adopt super-critical fluid extraction to extract, Extraction solvent is carbon dioxide, and Extracting temperature is 55 DEG C, and pressure is 17MPa, obtains Aloe extract after extracting 40min;
Get 15kg Cortex Eucommiae raw material, clean after drying and add 95% alcohol reflux 3 times, extraction time first time is 70min, for the second time, extraction time third time is 55min, each Extracting temperature is 60 DEG C, and each extraction adds 95% ethanol of 95L, has extracted rear filtration three times, then merge extractive liquid, concentrating under reduced pressure becomes the concentrated solution of relative density 1.20, and dry with spray drying method, the inlet temperature of spray-drying process is 140 DEG C, leaving air temp is 70 DEG C, obtains Cortex Eucommiae extract;
(2) get the extract mix homogeneously of following weight portion: Rhizoma Curcumae Longae extract 72 parts, Ganoderma extract 43 parts, Herba Dendrobii extract 17 parts, Rhizoma Polygoni Cuspidati extract 36 parts, Cortex Magnoliae Officinalis extract 12 parts, Aloe extract 4 parts, the Cortex Eucommiae extracts 20 parts.
Embodiment 4
Mixed extract in embodiment 1 step (2) is made pill.
Embodiment 5
Mixed extract in embodiment 2 step (2) is made tablet.
Embodiment 6
Mixed extract in embodiment 3 step (2) is made granule.
Embodiment 7
Mixed extract in embodiment 3 step (2) is made capsule.
Embodiment 8
Mixed extract in embodiment 3 step (2) is made electuary.
Comparative example 1
Get 15kg Rhizoma Curcumae Longae raw material, clean and dry rear pulverizing, add the alcohol reflux 3 times that volumetric concentration is 85%, extraction time first time is 130min, for the second time, extraction time third time is 90min, each Extracting temperature is 70 DEG C, each extraction adds ethanol 150L, extracted rear filtration three times, then merge extractive liquid, concentrating under reduced pressure becomes the concentrated solution of relative density 1.20, dry with spray drying method, the inlet temperature of spray-drying process is 140 ~ 160 DEG C, and leaving air temp is 70 ~ 80 DEG C, obtains Rhizoma Curcumae Longae extract;
Get 15kg Ganoderma raw material, clean the Ganoderma tablet being cut into 1mm after drying, Ganoderma tablet to be added in the ethanol of 75% reflux, extract, 3 times, extraction time first time is 100min, second time extraction time is 80min, extraction time third time is 60min, and Extracting temperature is 55 DEG C, adds 75% ethanol of 100L during each extraction; Extracted rear filtration three times, then merge extractive liquid, concentrating under reduced pressure becomes the concentrated solution of relative density 1.22, and rear spray drying method is dry, and the inlet temperature of spray-drying process is 160 DEG C, and air outlet temperature is 80 DEG C, obtains Ganoderma extract;
Get Herba Dendrobii raw material 15kg, clean and dry rear pulverizing, be added to the water decoction 4 times, decocting time is 80min for the first time, second time and for the third time decocting time are 70min, 4th time decocting time is 55min, the weight at every turn added water is 6-7 times of Herba Dendrobii raw material weight, after decoction, decoction liquor is filtered and merge, at 45 DEG C, concentrating under reduced pressure becomes the concentrated solution of relative density 1.20, and dry with spray drying method, the inlet temperature of spray-drying process is 140 ~ 160 DEG C, leaving air temp is 70 ~ 80 DEG C, and drying obtains Herba Dendrobii extract;
Get 20kg Rhizoma Polygoni Cuspidati raw material, clean after drying and add 75% alcohol reflux 2 times, extraction time first time is 100min, second time extraction time is 70min, each Extracting temperature is 50 DEG C, and each extraction amount of alcohol added is 170L, filtration after extracted twice completes, then merge extractive liquid, concentrating under reduced pressure becomes the concentrated solution of relative density 1.20, and dry with spray drying method, the inlet temperature of spray-drying process is 140 ~ 160 DEG C, leaving air temp is 70 ~ 80 DEG C, obtains Rhizoma Polygoni Cuspidati extract;
Get 20kg magnolia bark raw material, clean after drying and be added to the water decoction 3 times, decocting time is 170min for the first time, second time decocting time is 110min, and decocting time is 55min for the third time, decocts 12 times that the weight added water is magnolia bark raw material at every turn, at 50 DEG C, concentrating under reduced pressure becomes the concentrated solution of relative density 1.20, uses spray drying method dry, the inlet temperature of spray-drying process is 140 ~ 160 DEG C, and leaving air temp is 70 ~ 80 DEG C, obtains Cortex Magnoliae Officinalis extract;
Get aloe raw material 20kg, smash after cleaning, adopt super-critical fluid extraction to extract, Extraction solvent is carbon dioxide, and Extracting temperature is 60 DEG C, and pressure is 20MPa, obtains Aloe extract after extracting 50min;
Get 15kg Cortex Eucommiae raw material, clean after drying and add 85% alcohol reflux 3 times, extraction time first time is 80min, for the second time, extraction time third time is 65min, each Extracting temperature is 60 DEG C, each extraction adds 85% ethanol of 105L, extract rear filtration three times, then merge extractive liquid, concentrating under reduced pressure becomes the concentrated solution of relative density 1.20, and dry with spray drying method, the inlet temperature of spray-drying process is 140 ~ 160 DEG C, leaving air temp is 70 ~ 80 DEG C, obtains Cortex Eucommiae extract;
(2) get the extract mix homogeneously of following weight portion: Rhizoma Curcumae Longae extract 62 parts, Ganoderma extract 40 parts, Herba Dendrobii extract 20 parts, Rhizoma Polygoni Cuspidati extract 34 parts, Cortex Magnoliae Officinalis extract 16 parts, Aloe extract 1 part, the Cortex Eucommiae extracts 24 parts.
Comparative example 2
Get 15kg Rhizoma Curcumae Longae raw material, clean and dry rear pulverizing, add the alcohol reflux 3 times that volumetric concentration is 75%, extraction time first time is 110min, for the second time, extraction time third time is 70min, each Extracting temperature is 65 DEG C, each extraction adds ethanol 140L, extracted rear filtration three times, then merge extractive liquid, concentrating under reduced pressure becomes the concentrated solution of relative density 1.20, dry with spray drying method, the inlet temperature of spray-drying process is 140 ~ 160 DEG C, and leaving air temp is 70 ~ 80 DEG C, obtains Rhizoma Curcumae Longae extract;
Get 15kg Ganoderma raw material, clean the Ganoderma tablet being cut into 2mm after drying, Ganoderma tablet to be added in the ethanol of 95% reflux, extract, 3 times, extraction time first time is 120min, second time extraction time is 100min, extraction time third time is 80min, and Extracting temperature is 60 DEG C, adds 95% ethanol of 125L during each extraction; Extracted rear filtration three times, then merge extractive liquid, concentrating under reduced pressure becomes the concentrated solution of relative density 1.20, and rear spray drying method is dry, and the inlet temperature of spray-drying process is 160 DEG C, and air outlet temperature is 80 DEG C, obtains Ganoderma extract;
Get Herba Dendrobii raw material 15kg, clean and dry rear pulverizing, be added to the water decoction 4 times, decocting time is 60min for the first time, second time and for the third time decocting time are 50min, 4th time decocting time is 35min, the weight at every turn added water is 4 times of Herba Dendrobii raw material weight, after decoction, decoction liquor is filtered and merge, at 45 DEG C, concentrating under reduced pressure becomes the concentrated solution of relative density 1.20, and dry with spray drying method, the inlet temperature of spray-drying process is 140 ~ 160 DEG C, leaving air temp is 70 ~ 80 DEG C, and drying obtains Herba Dendrobii extract;
Get 20kg Rhizoma Polygoni Cuspidati raw material, clean after drying and add 65% alcohol reflux 2 times, extraction time first time is 80min, second time extraction time is 50min, each Extracting temperature is 40 DEG C, and each extraction amount of alcohol added is 150L, filtration after extracted twice completes, then merge extractive liquid, concentrating under reduced pressure becomes the concentrated solution of relative density 1.20, and dry with spray drying method, the inlet temperature of spray-drying process is 140 ~ 160 DEG C, leaving air temp is 70 ~ 80 DEG C, obtains Rhizoma Polygoni Cuspidati extract;
Get 20kg magnolia bark raw material, clean after drying and be added to the water decoction 3 times, decocting time is 150min for the first time, second time decocting time is 90min, and decocting time is 35min for the third time, decocts 7 times that the weight added water is magnolia bark raw material at every turn, at 45 DEG C, concentrating under reduced pressure becomes the concentrated solution of relative density 1.20, uses spray drying method dry, the inlet temperature of spray-drying process is 140 ~ 160 DEG C, and leaving air temp is 70 ~ 80 DEG C, obtains Cortex Magnoliae Officinalis extract;
Get aloe raw material 20kg, smash after cleaning, adopt super-critical fluid extraction to extract, Extraction solvent is carbon dioxide, and Extracting temperature is 50 DEG C, and pressure is 14MPa, obtains Aloe extract after extracting 30min;
Get 15kg Cortex Eucommiae raw material, clean after drying and add 95% alcohol reflux 3 times, extraction time first time is 60min, for the second time, extraction time third time is 45min, each Extracting temperature is 55 DEG C, each extraction adds 95% ethanol of 85L, extract rear filtration three times, then merge extractive liquid, concentrating under reduced pressure becomes the concentrated solution of relative density 1.20, and dry with spray drying method, the inlet temperature of spray-drying process is 140 ~ 160 DEG C, leaving air temp is 70 ~ 80 DEG C, obtains Cortex Eucommiae extract;
(2) get the extract mix homogeneously of following weight portion: Rhizoma Curcumae Longae extract 78 parts, Ganoderma extract 47 parts, Herba Dendrobii extract 13 parts, Rhizoma Polygoni Cuspidati extract 40 parts, Cortex Magnoliae Officinalis extract 9 parts, Aloe extract 9 parts, the Cortex Eucommiae extracts 17 parts.
Test example 1 testing drug enhancing immunity effect test
1.1 laboratory animals:
Kunming kind female mice, body weight 18-22g.
1.2 pilot project
Carry out internal organs/body weight ratio, Turnover of Mouse Peritoneal Macrophages engulf chicken red blood cell experiment, delayed allergy, half hemolysis value (HC50) measure and antibody-producting cell detect, carbonic clearance experiment detect, ConA induction mouse spleen lymphocyte transformation experiment and NK cytoactive detect.
1.3 test groupings:
Mice is divided into matched group 1, matched group 2, blank group and Chinese medicine composition experimental group of the present invention 1,2,3 groups at random.The medicine of matched group 1 medicine used for providing in comparative example 1, the medicine that matched group 2 medicine used provides for comparative example 2, Chinese medicine composition experimental group of the present invention (under be called for short experimental group) 1,2,3 group is successively with the medicine that embodiment 1,2,3 provides, and blank group is gavage pure water.Dosage is 0.4g/kg.bw, once a day, and gavage 30 days.
1.4 test methods:
Test about the requirement of enhancing immunity functional check method according in Ministry of Public Health " health food inspection and assessment technical specification " (2003 editions), specifically comprise internal organs/weight ratio pH-value determination pH, the mouse spleen lymphocyte transformation experiment (mtt assay) of ConA induction, mouse DTH experiment (ear swelling method) of dinitrofluorobenzene induction, antibody-producting cell detects (Jerne improves slide method), the mensuration (Hemagglutination Method) of serum hemolysin, mice carbonic clearance is tested, Turnover of Mouse Peritoneal Macrophages engulfs chicken red blood cell experiment (half intracorporal method), NK cytoactive detection (LDH algoscopy).
1.5 result of the tests:
Table 1 is on the impact of Mouse Weight
| Group | Animal number of elements | Initial stage body weight (g) | Body weight in mid-term (g) | Body weight in latter stage (g) |
| Experimental group 1 | 10 | 19.1±0.1 | 31.2±0.5 | 36.8±1.3 |
| Experimental group 2 | 10 | 19.2±0.4 | 32.4±0.2 | 36.5±0.7 |
| Experimental group 3 | 10 | 19.1±0.2 | 31.6±0.2 | 37.4±1.2 |
| Matched group 1 | 10 | 18.9±0.1 | 30.1±1.1 | 35.9±0.8 |
| Matched group 2 | 10 | 19.1±0.2 | 32.2±1.4 | 37.8±1.5 |
| Blank group | 10 | 19.3±0.5 | 30.3±0.6 | 36.4±1.1 |
Table 2 is on the impact of mice organs/body weight ratio
From table 1,2 results, medicine provided by the invention has no significant effect Mouse Weight and organ weights
Table 3 is on the impact of mice delayed allergy (DTH)
| Group | Animal number of elements | Swelling degree of the paw (mm) | P value |
| Experimental group 1 | 10 | 0.32±0.11 | ＜0.05 |
| Experimental group 2 | 10 | 0.32±0.08 | ＜0.05 |
| Experimental group 3 | 10 | 0.32±0.12 | ＜0.05 |
| Matched group 1 | 10 | 0.34±0.07 | ＜0.05 |
| Matched group 2 | 10 | 0.37±0.14 | ＜0.05 |
| Blank group | 10 | 0.21±0.09 | - |
Table 4 on mouse lymphocyte conversion capability test impact
As shown in Table 4, each group medicine does not make significant difference to mouse lymphocyte multiplication capacity.
Table 5 is on the impact of mouse antibodies cellulation number
As shown in Table 5, medicine provided by the invention and matched group have significant difference.
Table 6 is on the impact of mice half hemolysis value (HC50)
| Group | Animal number of elements | Half hemolysis value | P value |
| (HC50) | |||
| Experimental group 1 | 10 | 187.2±26.5 | ＜0.01 |
| Experimental group 2 | 10 | 182.2±17.1 | ＜0.01 |
| Experimental group 3 | 10 | 194.2±24.1 | ＜0.01 |
| Matched group 1 | 10 | 146.2±23.2 | ＜0.01 |
| Matched group 2 | 10 | 147.2±18.1 | ＜0.01 |
| Blank group | 10 | 140.2±19.2 | - |
As shown in Table 5, medicine provided by the invention and matched group have significant difference.
Table 7 is on the impact of mouse monokaryon-macrophage carbonic clearance phagocytic index
| Group | Animal number of elements | Hugely bite index (a) | P value |
| Experimental group 1 | 10 | 8.10±0.92 | ＜0.01 |
| Experimental group 2 | 10 | 8.14±0.87 | ＜0.01 |
| Experimental group 3 | 10 | 8.32±0.67 | ＜0.01 |
| Matched group 1 | 10 | 5.79±0.58 | ＜0.01 |
| Matched group 2 | 10 | 5.93±072 | ＜0.01 |
| Blank group | 10 | 5.48±0.56 | - |
Table 8 engulfs the impact of chicken red blood cell ability to mouse macrophage
| Group | Animal number of elements | Hugely bite index (a) | P value |
| Experimental group 1 | 10 | 2.32±0.21 | ＜0.01 |
| Experimental group 2 | 10 | 2.33±0.18 | ＜0.01 |
| Experimental group 3 | 10 | 2.36±0.19 | ＜0.01 |
| Matched group 1 | 10 | 1.36±0.25 | ＜0.01 |
| Matched group 2 | 10 | 1.42±0.27 | ＜0.01 |
| Blank group | 10 | 1.34±0.23 | - |
Table 9 is on the impact of NK cells in mice activity
| Group | Animal number of elements | NK cytoactive (%) | P value |
| Experimental group 1 | 10 | 42.5±9.2 | ＞0.05 |
| Experimental group 2 | 10 | 44.6±10.4 | ＞0.05 |
| Experimental group 3 | 10 | 41.2±9.2 | ＞0.05 |
| Matched group 1 | 10 | 46.5±8.7 | ＞0.05 |
| Matched group 2 | 10 | 42.8±11.6 | ＞0.05 |
| Blank group | 10 | 45.1±8.2 | - |
Conclusion: medicine provided by the invention significantly can promote mouse macrophage and engulf chicken red blood cell ability, monocytes/macrophages carbonic clearance phagocytic activity, antibody-producting cell ability, thus improves immunocompetence.
Test example 2 testing drug regulates systema cariovasculare functional test
Experimental animal divides into groups with test example 1 with test
Dosage is 0.4g/kg.bw, once a day, and gavage 30 days.After last administration, 30min adopts rats in vitro common carotid artery-external jugular vein the method for bypass, rat is lain on the back fixing, intubate after separation trachea, be separated right outside common carotid artery and left external jugular vein, 6cm long filament line is put in polyethylene tube, left external jugular vein is inserted in pipe one end, then to injecting heparin normal saline (50IU/ml) in polyethylene tube, be full of the anticoagulant of rear injection heparin (50UI/kg), then the pipe other end is inserted right common carotid artery, open bulldog clamp, blood flow to polyethylene tube from right common carotid artery, returns left external jugular vein.Decontrol Herba Clinopodii in after blood flow 10min, remove rapidly silk thread and weigh.Gross weight-silk thread weight=wet weight of thrombus.The results are shown in following table:
Table 10 is on the impact of wet weight of thrombus
| Group | Animal number of elements | Wet weight of thrombus (mg) | P value |
| Experimental group 1 | 10 | 12.1 | ＜0.01 |
| Experimental group 2 | 10 | 11.4 | ＜0.01 |
| Experimental group 3 | 10 | 10.8 | ＜0.01 |
| Matched group 1 | 10 | 33.2 | ＜0.01 |
| Matched group 2 | 10 | 32.5 | ＜0.01 |
| Blank group | 10 | 37.8 | - |
From table 10 testing result, Chinese medicine provided by the invention has good thrombolytic effect, can play thromboembolism preventing effect.
Above a kind of Chinese medicine contributing to enhancing immunity provided by the present invention and preparation method thereof is described in detail.Apply specific case herein to set forth principle of the present invention and embodiment, the explanation of above embodiment just understands core concept of the present invention for helping.It should be pointed out that for those skilled in the art, under the premise without departing from the principles of the invention, some improvement and modification can also be carried out to the present invention.These improve and modify and also should fall in the protection domain of the claims in the present invention.
Claims (5)
1. one kind contributes to the Chinese medicine of enhancing immunity, its effective ingredient is made up of the component of following weight portion: Rhizoma Curcumae Longae extract 65-75 part, Ganoderma extract 42-45 part, Herba Dendrobii extract 15-18 part, Rhizoma Polygoni Cuspidati extract 36-38 part, Cortex Magnoliae Officinalis extract 11-14 part, Aloe extract 2-7 part, the Cortex Eucommiae extracts 19-22 part;
Described Rhizoma Curcumae Longae extract prepares by the following method: get Rhizoma Curcumae Longae raw material, clean and dry rear pulverizing, add 75% alcohol reflux 3 times, extraction time first time is 120min, and extraction time is 80min for the second time, for the third time, each Extracting temperature is 65 DEG C, during each extraction, the rate of charge of Rhizoma Curcumae Longae raw material and ethanol is: every 15kg Rhizoma Curcumae Longae raw material adds 75% ethanol of 140L, has extracted rear filtration three times, then merge extractive liquid, dry after concentrating under reduced pressure, obtain Rhizoma Curcumae Longae extract;
Described Ganoderma extract prepares by the following method: get Ganoderma raw material, clean the Ganoderma tablet being cut into 1-3mm after drying, Ganoderma tablet to be added in the ethanol of 85% reflux, extract, 3 times, extraction time first time is 110min, second time extraction time is 90min, extraction time third time is 70min, and Extracting temperature is 60 DEG C, and during each extraction, the rate of charge of Ganoderma raw material and ethanol is: 85% ethanol adding 115L in every 15kg Ganoderma raw material; Extract rear filtration three times, then merge extractive liquid, dry after concentrating under reduced pressure, obtain Ganoderma extract;
Described Herba Dendrobii extract prepares by the following method: get Herba Dendrobii raw material, clean and dry rear pulverizing, be added to the water decoction 4 times, decocting time is 70min for the first time, second time and for the third time decocting time are 60min, and the 4th time decocting time is 45min, and the weight at every turn added water is 6-7 times of Herba Dendrobii raw material weight, decoction liquor filtered after decoction and merge, at 45-50 DEG C, concentrating under reduced pressure, drying obtain Herba Dendrobii extract;
Described Rhizoma Polygoni Cuspidati extract prepares by the following method: get Rhizoma Polygoni Cuspidati raw material, clean after drying and add 65% alcohol reflux 2 times, extraction time first time is 90min, second time extraction time is 60min, and each Extracting temperature is 45 DEG C, and during each extraction, the rate of charge of Rhizoma Polygoni Cuspidati raw material and ethanol is: every 20kg Rhizoma Polygoni Cuspidati raw material adds 65% ethanol of 160L, filtration after extracted twice completes, then merge extractive liquid, dry after concentrating under reduced pressure, obtain Rhizoma Polygoni Cuspidati extract;
Described Cortex Magnoliae Officinalis extract prepares by the following method: get magnolia bark raw material, clean after drying and be added to the water decoction 3 times, decocting time is 160min for the first time, second time decocting time is 100min, decocting time is 45min for the third time, the weight that each decoction adds water is 9-10 times of magnolia bark raw material, and at 45-50 DEG C, concentrating under reduced pressure, drying obtain Cortex Magnoliae Officinalis extract;
Described Aloe extract prepares by the following method: get aloe raw material, smashes after cleaning, and adopts super-critical fluid extraction to extract, Extraction solvent is carbon dioxide, Extracting temperature is 55 DEG C, and pressure is 16-18MPa, obtains Aloe extract after extracting 40min;
Described Cortex Eucommiae extract prepares by the following method: get Cortex Eucommiae raw material, clean after drying and add 95% alcohol reflux 3 times, extraction time first time is 70min, extraction time is 55min for the second time, for the third time, and each Extracting temperature is 60 DEG C, and during each extraction, the rate of charge of Cortex Eucommiae raw material and ethanol is: every 15kg Cortex Eucommiae adds 95% ethanol of 95L, extract rear filtration three times, then merge extractive liquid, dry after concentrating under reduced pressure, obtain Cortex Eucommiae extract.
2. the Chinese medicine contributing to enhancing immunity according to claim 1 and 2, it is characterized in that, described middle the effective elements of the medicine is made up of the component of following weight portion: Rhizoma Curcumae Longae extract 72 parts, Ganoderma extract 43 parts, Herba Dendrobii extract 17 parts, Rhizoma Polygoni Cuspidati extract 36 parts, Cortex Magnoliae Officinalis extract 12 parts, Aloe extract 4 parts, the Cortex Eucommiae extracts 20 parts.
3. the Chinese medicine contributing to enhancing immunity according to claim 1 and 2, is characterized in that, the relative density of extracting solution after concentrating under reduced pressure is 1.20 ~ 1.23.
4. contribute to a preparation method for the Chinese medicine of enhancing immunity described in claim 1, it is characterized in that, in turn include the following steps:
(1) extract preparation:
Get Rhizoma Curcumae Longae raw material, clean and dry rear pulverizing, add 75% alcohol reflux 3 times, extraction time first time is 120min, and extraction time is 80min for the second time, for the third time, each Extracting temperature is 65 DEG C, during each extraction, the rate of charge of Rhizoma Curcumae Longae raw material and ethanol is: every 15kg Rhizoma Curcumae Longae raw material adds 75% ethanol of 140L, has extracted rear filtration three times, then merge extractive liquid, dry after concentrating under reduced pressure, obtain Rhizoma Curcumae Longae extract;
Get Ganoderma raw material Ganoderma raw material, clean the Ganoderma tablet being cut into 1-3mm after drying, Ganoderma tablet to be added in the ethanol of 85% reflux, extract, 3 times, extraction time first time is 110min, second time extraction time is 90min, extraction time third time is 70min, and Extracting temperature is 60 DEG C, and during each extraction, the rate of charge of Ganoderma raw material and ethanol is: 85% ethanol adding 115L in every 15kg Ganoderma raw material; Extract rear filtration three times, then merge extractive liquid, dry after concentrating under reduced pressure, obtain Ganoderma extract;
Get Herba Dendrobii raw material, clean and dry rear pulverizing, be added to the water decoction 4 times, decocting time is 70min for the first time, second time and for the third time decocting time are 60min, and the 4th time decocting time is 45min, and the weight at every turn added water is 6-7 times of Herba Dendrobii raw material weight, decoction liquor filtered after decoction and merge, at 45-50 DEG C, concentrating under reduced pressure, drying obtain Herba Dendrobii extract;
Get Rhizoma Polygoni Cuspidati raw material, clean after drying and add 65% alcohol reflux 2 times, extraction time first time is 90min, second time extraction time is 60min, and each Extracting temperature is 45 DEG C, and during each extraction, the rate of charge of Rhizoma Polygoni Cuspidati raw material and ethanol is: every 20kg Rhizoma Polygoni Cuspidati raw material adds 65% ethanol of 160L, filtration after extracted twice completes, then merge extractive liquid, dry after concentrating under reduced pressure, obtain Rhizoma Polygoni Cuspidati extract;
Get magnolia bark raw material, clean after drying and be added to the water decoction 3 times, decocting time is 160min for the first time, second time decocting time is 100min, decocting time is 45min for the third time, the weight that each decoction adds water is 9-10 times of magnolia bark raw material, and at 45-50 DEG C, concentrating under reduced pressure, drying obtain Cortex Magnoliae Officinalis extract;
Get aloe raw material, smash after cleaning, adopt super-critical fluid extraction to extract, Extraction solvent is carbon dioxide, and Extracting temperature is 55 DEG C, and pressure is 16-18MPa, obtains Aloe extract after extracting 40min;
Get Cortex Eucommiae raw material, clean after drying and add 95% alcohol reflux 3 times, extraction time first time is 70min, extraction time is 55min for the second time, for the third time, and each Extracting temperature is 60 DEG C, and during each extraction, the rate of charge of Cortex Eucommiae raw material and ethanol is: every 15kg Cortex Eucommiae adds 95% ethanol of 95L, extract rear filtration three times, then merge extractive liquid, dry after concentrating under reduced pressure, obtain Cortex Eucommiae extract;
(2) the extract mix homogeneously of following weight portion is got: Rhizoma Curcumae Longae extract 65-75 part, Ganoderma extract 42-45 part, Herba Dendrobii extract 15-18 part, Rhizoma Polygoni Cuspidati extract 36-38 part, Cortex Magnoliae Officinalis extract 11-14 part, Aloe extract 2-7 part, the Cortex Eucommiae extracts 19-22 part;
(3) mixed extract is made pharmaceutically acceptable oral formulations.
5. the preparation method contributing to the Chinese medicine of enhancing immunity according to claim 4, is characterized in that, described oral formulations comprises capsule, granule, electuary, tablet and pill.
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| CN108813245A (en) * | 2018-05-25 | 2018-11-16 | 山西植美馨生物科技有限公司 | A kind of compound flax fermentation oral liquid |
| IT201900008259A1 (en) * | 2019-06-06 | 2020-12-06 | Nutrilinea S R L | COMPOSITION INCLUDING EXTRACTS OF EUCOMMIA ULMOIDES, CROCUS SATIVUS AND MAGNOLIA OFFICINALIS AND ITS USE IN THE TREATMENT OF SLEEP DISORDERS |
| WO2020245779A1 (en) * | 2019-06-06 | 2020-12-10 | Nutrilinea S.r.l. | Composition comprising extracts of eucommia ulmoides, crocus sativus and/or magnolia officinalis and the use thereof in the treatment of sleep disorders |
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| CN104623541B (en) | 2016-01-20 |
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