CN104622989A - Traditional Chinese medicine compound sterilizing agent and preparation method thereof - Google Patents
Traditional Chinese medicine compound sterilizing agent and preparation method thereof Download PDFInfo
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- CN104622989A CN104622989A CN201510021105.XA CN201510021105A CN104622989A CN 104622989 A CN104622989 A CN 104622989A CN 201510021105 A CN201510021105 A CN 201510021105A CN 104622989 A CN104622989 A CN 104622989A
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Abstract
The invention discloses a traditional Chinese medicine compound sterilizing agent. The traditional Chinese medicine compound sterilizing agent is prepared from the following components in percentage by mass: 60 to 80% of coptis chinensis, 10 to 30% of radix sophorae flavescentis and 10 to 20% of liquorice; the sum of the components in percentage by mass is 100%. The invention discloses a preparation method. The preparation method comprises the following steps: mixing coptis chinensis, radix sophorae flavescentis and liquorice powder; adding distilled water; extracting under the assistance of microwaves; filtering the extracted liquid to obtain the traditional Chinese medicine compound sterilizing agent. The traditional Chinese medicine compound sterilizing agent adopts water as a solvent, so that no pollution is caused, the safety is improved, and broad sterilizing performance is achieved; compared with the traditional solvent-based extraction, the preparation method of the traditional Chinese medicine compound sterilizing agent has the advantages that the novel microwave-resisted efficient extraction technology is carried out, and active ingredients of each Chinese herbal medicine can be fully dissolved out; the traditional Chinese medicine compound sterilizing agent is mild in preparation temperature, and thus the active ingredients can be prevented from being damaged.
Description
Technical field
The invention belongs to Chinese medicine biocide technical field, be specifically related to a kind of Chinese medicine compound biocide, the invention still further relates to the preparation method of this Chinese medicine compound biocide.
Background technology
China carries out the extracorporeal bacteria inhibitor test of Chinese medicine from the forties in 20th century, and demonstrating large quantities of Chinese medicine has inhibitory action to various antibacterial.The existing Chinese medicinal components for sterilizing has Herba Taraxaci, Herba Plantaginis, Herba Scutellariae Barbatae, Radix Glycyrrhizae, Herba Patriniae, Folium Artemisiae Argyi, Rhizoma Atractylodis, Rhizoma Coptidis, Herba Lysimachiae, Radix Sophorae Flavescentis, Flos Lonicerae, Rhizoma Polygoni Cuspidati, Radix Scutellariae etc., and most of chemical substances contained by it such as terpenes, alkaloid, phenols, polysaccharide etc. all have parasite killing or antibacterial activity.But single medicinal material sterilizing scope is little, effect is not remarkable.Compatibility between Chinese herbal medicine can reach the effect that single medicinal material cannot meet, and is conducive to improving drug effect, and the synergism after drug matching is also for the new Traditional Chinese medicine bacteriostatic agent of exploitation provides important evidence.But the drug matching in the past for sterilizing depends on Conventional wisdom or ancient prescription mostly, does not have scientific experiments foundation.Visible, using the stripping quantity of key component as evaluation index, formulation optimization carries out to herbal mixture very necessary.
Summary of the invention
The object of this invention is to provide a kind of Chinese medicine compound biocide, this biocide take water as solvent, and pollution-free, safety is good.
Another object of the present invention is to provide a kind of preparation method of Chinese medicine compound biocide.
The technical solution adopted in the present invention is, a kind of Chinese medicine compound biocide, composed of the following components by mass percentage: Rhizoma Coptidis 60%-80%, Radix Sophorae Flavescentis 10%-30%, Radix Glycyrrhizae 10%-20%, and above constituent mass percentage ratio sum is 100%.
Another technical scheme of the present invention is, a kind of preparation method of Chinese medicine compound biocide,
Specifically implement according to following steps:
Step 1, takes Rhizoma Coptidis 60%-80%, Radix Sophorae Flavescentis 10%-30%, Radix Glycyrrhizae 10%-20% by mass percentage respectively, and above constituent mass percentage ratio sum is 100%;
Step 2, adds distilled water by after Rhizoma Coptidis, Radix Sophorae Flavescentis, the mixing of Radix Glycyrrhizae powder, adopts microwave radiation exaraction, finally by extracting liquid filtering, obtain Chinese medicine compound biocide.
Feature of the present invention is also,
In step 1 before taking, pick Rhizoma Coptidis, Radix Sophorae Flavescentis, the thick rhizome of Radix Glycyrrhizae, removing fibrous root, puts into the 37-50 DEG C of drying of constant temperature blast drier; Pulverize and cross 80 mesh sieves with high speed Universalpulverizer, Rhizoma Coptidis, Radix Sophorae Flavescentis, Radix Glycyrrhizae powder respectively.
In step 2, distilled water and the volume mass of Rhizoma Coptidis, Radix Sophorae Flavescentis, Radix Glycyrrhizae mixture are than being 20-40mL/g.
In step 2, in microwave radiation exaraction, constant power is 800-1000w, at 40-60 DEG C, extract 40-65min.
The invention has the beneficial effects as follows,
Chinese medicine compound biocide of the present invention take water as solvent, pollution-free, and safety is good, and has broad-spectrum sterilization.
Compared to traditional solvent extraction, the preparation method of Chinese medicine compound biocide of the present invention adopts the new and effective abstraction technique of microwave-assisted, can guarantee the abundant stripping of the effective elements of the medicine in each taste; This Chinese medicine compound biocide preparation temperature is moderate, can ensure that effective ingredient is not destroyed.
Detailed description of the invention
Below in conjunction with detailed description of the invention, the present invention is described in detail.
Chinese medicine compound biocide of the present invention, composed of the following components by mass percentage: Rhizoma Coptidis 60%-80%, Radix Sophorae Flavescentis 10%-30%, Radix Glycyrrhizae 10%-20%, above constituent mass percentage ratio sum is 100%.
Rhizoma Coptidis is rich in the various alkaloids such as berberine, coptisine, 13-methyl-.psi.-coptisine., palm leaf coculine, Columbamine, the multiple alkaloids such as sophocarpine, sophoridine, the other matrine of dextrorotation, dextrorotation Iosmatrine are oxidized containing matrine, oxymatrine, N-in Radix Sophorae Flavescentis, all there is stronger sterilizing activity, the monarch drug for this reason in compound recipe.Radix Glycyrrhizae content 10%-20%, plays a role mainly as ministerial drug in this side.
Usage and dosage: effective oral dose of the present invention is Rhizoma Coptidis on the one 16 grams, Radix Sophorae Flavescentis 2 grams, 2 grams, Radix Glycyrrhizae, and adding distil water 567mL, constant power 1000w, extract 64.92min at 59.08 DEG C, filters, obtains Chinese medicine compound biocide, point takes for 3 ~ 4 times.
The preparation method of Chinese medicine compound biocide of the present invention, specifically implement according to following steps:
Step 1, picks Rhizoma Coptidis, Radix Sophorae Flavescentis, the thick rhizome of Radix Glycyrrhizae, and removing fibrous root, puts into the 37 DEG C of dryings of constant temperature blast drier; Pulverize and cross 80 mesh sieves with high speed Universalpulverizer, Rhizoma Coptidis, Radix Sophorae Flavescentis, Radix Glycyrrhizae powder respectively;
Step 2, takes Rhizoma Coptidis 80%-60%, Radix Sophorae Flavescentis 10%-30%, Radix Glycyrrhizae 10%-20% by mass percentage respectively, and above constituent mass percentage ratio sum is 100%;
Step 3, distilled water is added by after Rhizoma Coptidis, Radix Sophorae Flavescentis, the mixing of Radix Glycyrrhizae powder, distilled water is 20-40mL/g with the volume mass ratio of Rhizoma Coptidis, Radix Sophorae Flavescentis, Radix Glycyrrhizae mixture, adopt microwave radiation exaraction, constant power 800-1000w, at 40-60 DEG C, extract 40-65min, finally by extracting liquid filtering, obtain Chinese medicine compound biocide.
Adopt Bactericidal test, observe Chinese medicine compound biocide of the present invention to the inhibitory action of clinical common pathogenic bacteria.Take 33g Nutrient agar, add in 1000mL distilled water, heated and boiled is dissolved, 121 DEG C, under 102MPa sterilizing within 15 minutes, make culture medium, when temperature drops to 50 DEG C-60 DEG C, culture medium after sterilizing is poured in the culture dish of same method sterilizing by super-clean bench, lets cool, solidify, for subsequent use.To need the microbionation investigated on culture plate, then stick the filter paper being soaked with medicinal liquid, put into 37 DEG C of incubators, cultivate and observe inhibition zone size and fungistatic effect after 12-16 hour.
The display of antibacterial result, compound recipe antibacterial of the present invention to escherichia coli, staphylococcus aureus, staphylococcus epidermidis, staphylococcus saprophyticus is antibacterial all stronger inhibitory action, inhibition zone is respectively 2.30cm, 1.80cm, 2.50cm and 2.20cm.Compound recipe biocide of the present invention also has certain inhibition to Bacillus typhi.Chinese medicine compound antibacterial of the present invention has good inhibition to clinical common pathogenic bacteria, and antimicrobial spectrum is wider.
Three kinds of medical materials selected by the present invention are medicinal herbs most in use, therefore three's compatibility definite ingredients, safety is high, and quality is easy to control, and has the good suitability to separate sources medical material, therefore constant product quality of the present invention.
Clinical trial:
Model case one: thank certain, man, 61 years old, vomiting, heating, diarrhoea, times of defecation was slightly many, is yellow green mushy stool, daily Chinese medicine biocide 600mL of the present invention, and after two days, symptom alleviates, recovery from illness on the six.
Model case two: Zhang, female, 40 years old, vomiting, diarrhoea, every day defecated more than three times, daily Chinese medicine biocide 500mL of the present invention, and after two days, symptom obviously alleviates, recovery from illness on the five.
Model case three: Xu certain, man, 23 years old, occur vomiting and diarrhoea phenomenon after edible leftovers, daily Chinese medicine biocide 500mL of the present invention, fully recovered after two days.
Embodiment 1
Step 1, picks Rhizoma Coptidis, Radix Sophorae Flavescentis, the thick rhizome of Radix Glycyrrhizae, and removing fibrous root, puts into the 37 DEG C of dryings of constant temperature blast drier; Pulverize and cross 80 mesh sieves with high speed Universalpulverizer, Rhizoma Coptidis, Radix Sophorae Flavescentis, Radix Glycyrrhizae powder respectively;
Step 2, takes Rhizoma Coptidis 80%, Radix Sophorae Flavescentis 10%, Radix Glycyrrhizae 10% by mass percentage respectively, and above constituent mass percentage ratio sum is 100%;
Step 3, distilled water is added by after Rhizoma Coptidis, Radix Sophorae Flavescentis, the mixing of Radix Glycyrrhizae powder, distilled water is 340mL/g with the volume mass ratio of Rhizoma Coptidis, Radix Sophorae Flavescentis, Radix Glycyrrhizae mixture, adopt microwave radiation exaraction, constant power 1000w, at 60 DEG C, extract 65min, finally by extracting liquid filtering, obtain Chinese medicine compound biocide.
Embodiment 2
Step 1, picks Rhizoma Coptidis, Radix Sophorae Flavescentis, the thick rhizome of Radix Glycyrrhizae, and removing fibrous root, puts into the 37 DEG C of dryings of constant temperature blast drier; Pulverize and cross 80 mesh sieves with high speed Universalpulverizer, Rhizoma Coptidis, Radix Sophorae Flavescentis, Radix Glycyrrhizae powder respectively;
Step 2, takes Rhizoma Coptidis 60%, Radix Sophorae Flavescentis 30%, Radix Glycyrrhizae 10% by mass percentage respectively, and above constituent mass percentage ratio sum is 100%;
Step 3, distilled water is added by after Rhizoma Coptidis, Radix Sophorae Flavescentis, the mixing of Radix Glycyrrhizae powder, distilled water is 40mL/g with the volume mass ratio of Rhizoma Coptidis, Radix Sophorae Flavescentis, Radix Glycyrrhizae mixture, adopt microwave radiation exaraction, constant power 900w, at 45 DEG C, extract 50min, finally by extracting liquid filtering, obtain Chinese medicine compound biocide.
Embodiment 3
Step 1, picks Rhizoma Coptidis, Radix Sophorae Flavescentis, the thick rhizome of Radix Glycyrrhizae, and removing fibrous root, puts into the 37 DEG C of dryings of constant temperature blast drier; Pulverize and cross 80 mesh sieves with high speed Universalpulverizer, Rhizoma Coptidis, Radix Sophorae Flavescentis, Radix Glycyrrhizae powder respectively;
Step 2, takes Rhizoma Coptidis 70%, Radix Sophorae Flavescentis 10%, Radix Glycyrrhizae 20% by mass percentage respectively, and above constituent mass percentage ratio sum is 100%;
Step 3, distilled water is added by after Rhizoma Coptidis, Radix Sophorae Flavescentis, the mixing of Radix Glycyrrhizae powder, distilled water is 20mL/g with the volume mass ratio of Rhizoma Coptidis, Radix Sophorae Flavescentis, Radix Glycyrrhizae mixture, adopt microwave radiation exaraction, constant power 950w, at 50 DEG C, extract 40min, finally by extracting liquid filtering, obtain Chinese medicine compound biocide.
Embodiment 4
Step 1, picks Rhizoma Coptidis, Radix Sophorae Flavescentis, the thick rhizome of Radix Glycyrrhizae, and removing fibrous root, puts into the 37 DEG C of dryings of constant temperature blast drier; Pulverize and cross 80 mesh sieves with high speed Universalpulverizer, Rhizoma Coptidis, Radix Sophorae Flavescentis, Radix Glycyrrhizae powder respectively;
Step 2, takes Rhizoma Coptidis 65%, Radix Sophorae Flavescentis 20%, Radix Glycyrrhizae 15% by mass percentage respectively, and above constituent mass percentage ratio sum is 100%;
Step 3, distilled water is added by after Rhizoma Coptidis, Radix Sophorae Flavescentis, the mixing of Radix Glycyrrhizae powder, distilled water is 35mL/g with the volume mass ratio of Rhizoma Coptidis, Radix Sophorae Flavescentis, Radix Glycyrrhizae mixture, adopt microwave radiation exaraction, constant power 800w, at 40 DEG C, extract 60min, finally by extracting liquid filtering, obtain Chinese medicine compound biocide.
Claims (5)
1. a Chinese medicine compound biocide, is characterized in that, composed of the following components by mass percentage: Rhizoma Coptidis 60%-80%, Radix Sophorae Flavescentis 10%-30%, Radix Glycyrrhizae 10%-20%, and above constituent mass percentage ratio sum is 100%.
2. a preparation method for Chinese medicine compound biocide, is characterized in that, specifically implements according to following steps:
Step 1, takes Rhizoma Coptidis 60%-80%, Radix Sophorae Flavescentis 10%-30%, Radix Glycyrrhizae 10%-20% by mass percentage respectively, and above constituent mass percentage ratio sum is 100%;
Step 2, adds distilled water by after Rhizoma Coptidis, Radix Sophorae Flavescentis, the mixing of Radix Glycyrrhizae powder, adopts microwave radiation exaraction, finally by extracting liquid filtering, obtain Chinese medicine compound biocide.
3. the preparation method of Chinese medicine compound biocide according to claim 2, is characterized in that, in step 1 before taking, picks Rhizoma Coptidis, Radix Sophorae Flavescentis, the thick rhizome of Radix Glycyrrhizae, and removing fibrous root, puts into the 37-50 DEG C of drying of constant temperature blast drier; Pulverize and cross 80 mesh sieves with high speed Universalpulverizer, Rhizoma Coptidis, Radix Sophorae Flavescentis, Radix Glycyrrhizae powder respectively.
4. the preparation method of Chinese medicine compound biocide according to claim 2, is characterized in that, in step 2, distilled water and the volume mass of Rhizoma Coptidis, Radix Sophorae Flavescentis, Radix Glycyrrhizae mixture are than being 20-40mL/g.
5. the preparation method of Chinese medicine compound biocide according to claim 2, is characterized in that, in step 2, in microwave radiation exaraction, constant power is 800-1000w, at 40-60 DEG C, extract 40-65min.
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| CN106386868A (en) * | 2016-08-30 | 2017-02-15 | 宁夏沃福百瑞枸杞产业股份有限公司 | Special biopesticide for wolfberries and preparation method of biopesticide |
| CN108308203A (en) * | 2018-01-31 | 2018-07-24 | 南京紫源康医药科技有限公司 | A kind of preparation method of Chinese medicinal compound extract, extract and bacteria inhibiting composition |
| CN110025691A (en) * | 2019-04-30 | 2019-07-19 | 龙岩学院 | A kind of compound houttuynia cordata preparation and preparation method and application |
| CN115998814A (en) * | 2023-02-03 | 2023-04-25 | 四川新益峰生物制药有限公司 | Compound tilmicosin injection and preparation method thereof |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106386868A (en) * | 2016-08-30 | 2017-02-15 | 宁夏沃福百瑞枸杞产业股份有限公司 | Special biopesticide for wolfberries and preparation method of biopesticide |
| CN108308203A (en) * | 2018-01-31 | 2018-07-24 | 南京紫源康医药科技有限公司 | A kind of preparation method of Chinese medicinal compound extract, extract and bacteria inhibiting composition |
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| CN110025691A (en) * | 2019-04-30 | 2019-07-19 | 龙岩学院 | A kind of compound houttuynia cordata preparation and preparation method and application |
| CN115998814A (en) * | 2023-02-03 | 2023-04-25 | 四川新益峰生物制药有限公司 | Compound tilmicosin injection and preparation method thereof |
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Application publication date: 20150520 |