CN104620104B - 用于蛋白质纯化的亲和试剂 - Google Patents

用于蛋白质纯化的亲和试剂 Download PDF

Info

Publication number
CN104620104B
CN104620104B CN201380024731.3A CN201380024731A CN104620104B CN 104620104 B CN104620104 B CN 104620104B CN 201380024731 A CN201380024731 A CN 201380024731A CN 104620104 B CN104620104 B CN 104620104B
Authority
CN
China
Prior art keywords
amino
methyl
ethamine
ethyl
acid
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
CN201380024731.3A
Other languages
English (en)
Chinese (zh)
Other versions
CN104620104A (zh
Inventor
帕特里克·布朗
爱德华·R·沃茨
佩尔·哈布雷
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Leland Stanford Junior University
Impossible Foods Inc
Original Assignee
Leland Stanford Junior University
Impossible Foods Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Leland Stanford Junior University, Impossible Foods Inc filed Critical Leland Stanford Junior University
Publication of CN104620104A publication Critical patent/CN104620104A/zh
Application granted granted Critical
Publication of CN104620104B publication Critical patent/CN104620104B/zh
Expired - Fee Related legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/22—Affinity chromatography or related techniques based upon selective absorption processes
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
    • B01J20/281—Sorbents specially adapted for preparative, analytical or investigative chromatography
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K31/00—Medicinal preparations containing organic active ingredients
    • A61K31/33—Heterocyclic compounds
    • A61K31/395—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins
    • A61K31/53—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins having six-membered rings with three nitrogens as the only ring hetero atoms, e.g. chlorazanil, melamine
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
    • B01J20/281—Sorbents specially adapted for preparative, analytical or investigative chromatography
    • B01J20/286—Phases chemically bonded to a substrate, e.g. to silica or to polymers
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
    • B01J20/30—Processes for preparing, regenerating, or reactivating
    • B01J20/32—Impregnating or coating ; Solid sorbent compositions obtained from processes involving impregnating or coating
    • B01J20/3202—Impregnating or coating ; Solid sorbent compositions obtained from processes involving impregnating or coating characterised by the carrier, support or substrate used for impregnation or coating
    • B01J20/3204—Inorganic carriers, supports or substrates
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
    • B01J20/30—Processes for preparing, regenerating, or reactivating
    • B01J20/32—Impregnating or coating ; Solid sorbent compositions obtained from processes involving impregnating or coating
    • B01J20/3202—Impregnating or coating ; Solid sorbent compositions obtained from processes involving impregnating or coating characterised by the carrier, support or substrate used for impregnation or coating
    • B01J20/3206—Organic carriers, supports or substrates
    • B01J20/3208—Polymeric carriers, supports or substrates
    • B01J20/3212—Polymeric carriers, supports or substrates consisting of a polymer obtained by reactions otherwise than involving only carbon to carbon unsaturated bonds
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
    • B01J20/30—Processes for preparing, regenerating, or reactivating
    • B01J20/32—Impregnating or coating ; Solid sorbent compositions obtained from processes involving impregnating or coating
    • B01J20/3231—Impregnating or coating ; Solid sorbent compositions obtained from processes involving impregnating or coating characterised by the coating or impregnating layer
    • B01J20/3242—Layers with a functional group, e.g. an affinity material, a ligand, a reactant or a complexing group
    • B01J20/3244—Non-macromolecular compounds
    • B01J20/3246—Non-macromolecular compounds having a well defined chemical structure
    • B01J20/3248—Non-macromolecular compounds having a well defined chemical structure the functional group or the linking, spacer or anchoring group as a whole comprising at least one type of heteroatom selected from a nitrogen, oxygen or sulfur, these atoms not being part of the carrier as such
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
    • B01J20/30—Processes for preparing, regenerating, or reactivating
    • B01J20/32—Impregnating or coating ; Solid sorbent compositions obtained from processes involving impregnating or coating
    • B01J20/3231—Impregnating or coating ; Solid sorbent compositions obtained from processes involving impregnating or coating characterised by the coating or impregnating layer
    • B01J20/3242—Layers with a functional group, e.g. an affinity material, a ligand, a reactant or a complexing group
    • B01J20/3244—Non-macromolecular compounds
    • B01J20/3246—Non-macromolecular compounds having a well defined chemical structure
    • B01J20/3248—Non-macromolecular compounds having a well defined chemical structure the functional group or the linking, spacer or anchoring group as a whole comprising at least one type of heteroatom selected from a nitrogen, oxygen or sulfur, these atoms not being part of the carrier as such
    • B01J20/3251—Non-macromolecular compounds having a well defined chemical structure the functional group or the linking, spacer or anchoring group as a whole comprising at least one type of heteroatom selected from a nitrogen, oxygen or sulfur, these atoms not being part of the carrier as such comprising at least two different types of heteroatoms selected from nitrogen, oxygen or sulphur
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07D—HETEROCYCLIC COMPOUNDS
    • C07D251/00—Heterocyclic compounds containing 1,3,5-triazine rings
    • C07D251/02—Heterocyclic compounds containing 1,3,5-triazine rings not condensed with other rings
    • C07D251/12—Heterocyclic compounds containing 1,3,5-triazine rings not condensed with other rings having three double bonds between ring members or between ring members and non-ring members
    • C07D251/26—Heterocyclic compounds containing 1,3,5-triazine rings not condensed with other rings having three double bonds between ring members or between ring members and non-ring members with only hetero atoms directly attached to ring carbon atoms
    • C07D251/40—Nitrogen atoms
    • C07D251/54—Three nitrogen atoms
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07D—HETEROCYCLIC COMPOUNDS
    • C07D251/00—Heterocyclic compounds containing 1,3,5-triazine rings
    • C07D251/02—Heterocyclic compounds containing 1,3,5-triazine rings not condensed with other rings
    • C07D251/12—Heterocyclic compounds containing 1,3,5-triazine rings not condensed with other rings having three double bonds between ring members or between ring members and non-ring members
    • C07D251/26—Heterocyclic compounds containing 1,3,5-triazine rings not condensed with other rings having three double bonds between ring members or between ring members and non-ring members with only hetero atoms directly attached to ring carbon atoms
    • C07D251/40—Nitrogen atoms
    • C07D251/54—Three nitrogen atoms
    • C07D251/70—Other substituted melamines
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
    • G01N33/6818—Sequencing of polypeptides

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Urology & Nephrology (AREA)
  • Hematology (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Food Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Cell Biology (AREA)
  • Animal Behavior & Ethology (AREA)
  • Inorganic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Veterinary Medicine (AREA)
  • Public Health (AREA)
  • Epidemiology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Peptides Or Proteins (AREA)
  • Solid-Sorbent Or Filter-Aiding Compositions (AREA)
  • Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)
  • Cosmetics (AREA)
CN201380024731.3A 2012-03-16 2013-03-15 用于蛋白质纯化的亲和试剂 Expired - Fee Related CN104620104B (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201261611999P 2012-03-16 2012-03-16
US61/611,999 2012-03-16
PCT/US2013/032675 WO2013138793A2 (en) 2012-03-16 2013-03-15 Affinity reagants for protein purification

Publications (2)

Publication Number Publication Date
CN104620104A CN104620104A (zh) 2015-05-13
CN104620104B true CN104620104B (zh) 2017-03-01

Family

ID=49161945

Family Applications (1)

Application Number Title Priority Date Filing Date
CN201380024731.3A Expired - Fee Related CN104620104B (zh) 2012-03-16 2013-03-15 用于蛋白质纯化的亲和试剂

Country Status (6)

Country Link
US (2) US9737875B2 (https=)
EP (1) EP2825694A4 (https=)
CN (1) CN104620104B (https=)
HK (1) HK1206420A1 (https=)
IN (1) IN2014DN08481A (https=)
WO (1) WO2013138793A2 (https=)

Families Citing this family (26)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN112471322A (zh) 2011-07-12 2021-03-12 非凡食品有限公司 用于消费品的方法和组合物
EP3488703A1 (en) 2011-07-12 2019-05-29 Impossible Foods Inc. Methods and compositions for consumables
US10039306B2 (en) 2012-03-16 2018-08-07 Impossible Foods Inc. Methods and compositions for consumables
US20140220217A1 (en) 2011-07-12 2014-08-07 Maraxi, Inc. Method and compositions for consumables
WO2013138793A2 (en) * 2012-03-16 2013-09-19 Maraxi, Inc. Affinity reagants for protein purification
KR102381965B1 (ko) 2013-01-11 2022-04-04 임파서블 푸즈 인크. 소비재를 위한 방법 및 조성물
JP2016502868A (ja) 2013-01-11 2016-02-01 インポッシブル フーズ インコーポレイテッド コアセルベートを含む、乳成分非含有チーズ代替品
US20160222054A1 (en) * 2013-09-19 2016-08-04 Impossible Foods Inc. Ph switchable reagents and methods for their use
ES2782380T5 (es) 2013-10-30 2023-05-08 Univ Missouri Método de especificación y cultivo de linaje del músculo esquelético escalable
JP6759103B2 (ja) 2014-03-31 2020-09-23 インポッシブル フーズ インコーポレイテッド ひき肉レプリカ
US11976302B2 (en) 2017-05-06 2024-05-07 Upside Foods, Inc. Compositions and methods for increasing the culture density of a cellular biomass within a cultivation infrastructure
US11479792B2 (en) 2017-07-13 2022-10-25 Upside Foods, Inc. Compositions and methods for increasing the efficiency of cell cultures used for food production
CA3076249A1 (en) 2017-09-21 2019-03-28 The Brigham And Women's Hospital, Inc. Isolation, preservation, compositions and uses of extracts from justicia plants
US11051532B2 (en) 2017-09-22 2021-07-06 Impossible Foods Inc. Methods for purifying protein
CN108176386B (zh) * 2018-01-16 2020-11-06 武汉轻工大学 一种三聚氰胺键合色谱固定相及其制备方法和金属离子改性三聚氰胺键合色谱固定相
CN115413223A (zh) * 2019-12-12 2022-11-29 圭尔夫大学 植物基奶酪产品
IL294248A (en) 2019-12-31 2022-08-01 Air Protein Inc High protein food compositions
CN115397255A (zh) 2020-03-02 2022-11-25 空气蛋白公司 结构化高蛋白肉类似物组合物
US12011016B2 (en) 2020-09-14 2024-06-18 Impossible Foods Inc. Protein methods and compositions
CA3191387A1 (en) 2020-09-30 2022-04-07 Nobell Foods, Inc. Recombinant milk proteins and food compositions comprising the same
US10894812B1 (en) 2020-09-30 2021-01-19 Alpine Roads, Inc. Recombinant milk proteins
US10947552B1 (en) 2020-09-30 2021-03-16 Alpine Roads, Inc. Recombinant fusion proteins for producing milk proteins in plants
AU2021369580A1 (en) 2020-10-28 2023-05-11 Pioneer Hi-Bred International, Inc. Leghemoglobin in soybean
EP4418874A4 (en) 2021-10-19 2025-09-24 Eat Scifi Inc PLANT/ANIMAL CELL BASED HYBRID MEAT SUBSTITUTE
US20250340925A1 (en) * 2022-04-11 2025-11-06 Memorial Sloan-Kettering Cancer Center Novel bead link (blink) method for molecular archiving of dna
WO2023240152A1 (en) 2022-06-07 2023-12-14 Upside Foods, Inc. Engineering cell lines capable of proliferation in growth factor free media formulations

Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5582988A (en) * 1994-09-15 1996-12-10 Johnson & Johnson Clinical Diagnostics, Inc. Methods for capture and selective release of nucleic acids using weakly basic polymer and amplification of same
US20030166002A1 (en) * 2001-12-12 2003-09-04 Young-Tae Chang Triazine library with linkers
WO2005049653A1 (en) * 2003-07-14 2005-06-02 Genway Biotech, Inc. Affinity separation composition and method
US20080261202A1 (en) * 2002-12-16 2008-10-23 Invitrogen Corporation Tagged Polyfunctional Reagents Capable of Reversibly Binding Target Substances in a pH-dependent Manner
US20090004641A1 (en) * 2002-09-12 2009-01-01 Invitrogen Corporation Site-specific labeling of affinity peptides in fusion proteins
US20100173889A1 (en) * 2008-10-23 2010-07-08 Gruenenthal Gmbh Substituted Pyrimidine and Triazine Compounds
WO2011012302A1 (en) * 2009-07-28 2011-02-03 Instraction Gmbh Specific sorbent for binding proteins and peptides, and separation method using the same

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1123305A1 (en) 1998-10-19 2001-08-16 The Board Of Trustees Of The Leland Stanford Junior University Dna-templated combinatorial library chemistry
CA2394727A1 (en) * 1999-12-28 2001-07-05 Pharmacopeia, Inc. Pyrimidine and triazine kinase inhibitors
WO2013138793A2 (en) 2012-03-16 2013-09-19 Maraxi, Inc. Affinity reagants for protein purification

Patent Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5582988A (en) * 1994-09-15 1996-12-10 Johnson & Johnson Clinical Diagnostics, Inc. Methods for capture and selective release of nucleic acids using weakly basic polymer and amplification of same
US20030166002A1 (en) * 2001-12-12 2003-09-04 Young-Tae Chang Triazine library with linkers
US20090004641A1 (en) * 2002-09-12 2009-01-01 Invitrogen Corporation Site-specific labeling of affinity peptides in fusion proteins
US20080261202A1 (en) * 2002-12-16 2008-10-23 Invitrogen Corporation Tagged Polyfunctional Reagents Capable of Reversibly Binding Target Substances in a pH-dependent Manner
WO2005049653A1 (en) * 2003-07-14 2005-06-02 Genway Biotech, Inc. Affinity separation composition and method
US20100173889A1 (en) * 2008-10-23 2010-07-08 Gruenenthal Gmbh Substituted Pyrimidine and Triazine Compounds
WO2011012302A1 (en) * 2009-07-28 2011-02-03 Instraction Gmbh Specific sorbent for binding proteins and peptides, and separation method using the same

Also Published As

Publication number Publication date
EP2825694A2 (en) 2015-01-21
IN2014DN08481A (https=) 2015-05-08
WO2013138793A2 (en) 2013-09-19
US20170320041A1 (en) 2017-11-09
US20150087532A1 (en) 2015-03-26
EP2825694A4 (en) 2016-04-20
US9833768B2 (en) 2017-12-05
CN104620104A (zh) 2015-05-13
US9737875B2 (en) 2017-08-22
HK1206420A1 (en) 2016-01-08
WO2013138793A3 (en) 2014-12-11

Similar Documents

Publication Publication Date Title
CN104620104B (zh) 用于蛋白质纯化的亲和试剂
EP3022219B1 (en) Specific targeting of rna expanded repeat sequences
WO2006069610A3 (en) Process for the production of fine chemicals
US20160222054A1 (en) Ph switchable reagents and methods for their use
WO2009091518A3 (en) Isolated novel nucleic acid and protein molecules from corn and methods of using those molecules to generate transgenic plant with enhanced agronomic traits
EP2166102A3 (en) Expression enhancing intron sequences
EP2336309A3 (en) Polypeptides having endoglucanase activity and polynucleotides encoding same
WO2007087815A3 (en) Process for the control of production of fine chemicals
Wajid et al. Sulfamethoxazole-derived Schiff bases: synthesis, characterization, biological activities, molecular docking, DFT, and ADME studies
WO2005098015A3 (en) Process for the production of fine chemicals
DE10118698A1 (de) Verfahren zur Immobilisierung und damit hergestellte Anordnungen von Verbindungen auf einer planaren Oberfläche
JP2002506423A (ja) ペプチド模倣型大員環のコンビナトリアルライブラリーとそのための方法
Cho et al. Synthesis and screening of linear and cyclic oligocarbamate libraries. Discovery of high affinity ligands for GPIIb/IIIa
BRPI0512818A (pt) processos para a produção do respectivo produto quìmico fino e de um polipeptìdeo e para a identificação de um composto conferindo produção incrementada do respectivo produto quìmico fino, molécula de ácido nucleico isolada, construção de ácido nucleico, vetor, célula hospedeira, polipeptìdeo, anticorpo, tecido de planta, material de propagação, material colhido ou uma planta. métodos para selecionar agonistas e antagonistas da atividade de um polipeptìdeo, para identificar um produto gênico e para produzir uma composição agrìcola, composição e usos de uma molécula de ácido nucleico e de um polipeptìdeo e célula de planta, organela de célula de planta, tecido de planta, planta ou parte da mesma
WO2005014828A3 (en) Process for the production of fine chemicals in plants
EP1731615A1 (en) Cytoplasmic localization dna and rna
Mosca et al. Precise Positioning of Chiral Building Blocks in Monodisperse, Sequence‐Defined Polyamides
Wang et al. (−)-Bacillamide C: the convergent approach
Olsen et al. N-Alkylation reactions and indirect formation of amino functionalities in solid-phase synthesis
Kye et al. Synthesis and purification of self‐assembling peptide‐oligonucleotide conjugates by solid‐phase peptide fragment condensation
CN117143182A (zh) 一类多肽类仿酶催化剂及其制备方法
KR20110014561A (ko) 인공 효소
CN112661803A (zh) DNA编码化合物库构建中On-DNA 4-胺基喹唑啉化合物的合成方法
KR20160136695A (ko) 고리형 펩타이드의 사전 활성화 합성방법 및 이에 따라 합성된 고리형 펩타이드
JP2006258805A (ja) 基板、及びタンパク質の固定化方法

Legal Events

Date Code Title Description
C06 Publication
PB01 Publication
C10 Entry into substantive examination
SE01 Entry into force of request for substantive examination
GR01 Patent grant
GR01 Patent grant
CF01 Termination of patent right due to non-payment of annual fee
CF01 Termination of patent right due to non-payment of annual fee

Granted publication date: 20170301

Termination date: 20190315