CN104620104B - 用于蛋白质纯化的亲和试剂 - Google Patents

用于蛋白质纯化的亲和试剂 Download PDF

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Publication number
CN104620104B
CN104620104B CN201380024731.3A CN201380024731A CN104620104B CN 104620104 B CN104620104 B CN 104620104B CN 201380024731 A CN201380024731 A CN 201380024731A CN 104620104 B CN104620104 B CN 104620104B
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CN
China
Prior art keywords
amino
methyl
ethamine
ethyl
acid
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Expired - Fee Related
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CN201380024731.3A
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English (en)
Chinese (zh)
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CN104620104A (zh
Inventor
帕特里克·布朗
爱德华·R·沃茨
佩尔·哈布雷
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Leland Stanford Junior University
Impossible Foods Inc
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Leland Stanford Junior University
Impossible Foods Inc
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    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/22—Affinity chromatography or related techniques based upon selective absorption processes
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
    • B01J20/281—Sorbents specially adapted for preparative, analytical or investigative chromatography
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K31/00—Medicinal preparations containing organic active ingredients
    • A61K31/33—Heterocyclic compounds
    • A61K31/395—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins
    • A61K31/53—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins having six-membered rings with three nitrogens as the only ring hetero atoms, e.g. chlorazanil, melamine
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
    • B01J20/281—Sorbents specially adapted for preparative, analytical or investigative chromatography
    • B01J20/286—Phases chemically bonded to a substrate, e.g. to silica or to polymers
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
    • B01J20/30—Processes for preparing, regenerating, or reactivating
    • B01J20/32—Impregnating or coating ; Solid sorbent compositions obtained from processes involving impregnating or coating
    • B01J20/3202—Impregnating or coating ; Solid sorbent compositions obtained from processes involving impregnating or coating characterised by the carrier, support or substrate used for impregnation or coating
    • B01J20/3204—Inorganic carriers, supports or substrates
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
    • B01J20/30—Processes for preparing, regenerating, or reactivating
    • B01J20/32—Impregnating or coating ; Solid sorbent compositions obtained from processes involving impregnating or coating
    • B01J20/3202—Impregnating or coating ; Solid sorbent compositions obtained from processes involving impregnating or coating characterised by the carrier, support or substrate used for impregnation or coating
    • B01J20/3206—Organic carriers, supports or substrates
    • B01J20/3208—Polymeric carriers, supports or substrates
    • B01J20/3212—Polymeric carriers, supports or substrates consisting of a polymer obtained by reactions otherwise than involving only carbon to carbon unsaturated bonds
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
    • B01J20/30—Processes for preparing, regenerating, or reactivating
    • B01J20/32—Impregnating or coating ; Solid sorbent compositions obtained from processes involving impregnating or coating
    • B01J20/3231—Impregnating or coating ; Solid sorbent compositions obtained from processes involving impregnating or coating characterised by the coating or impregnating layer
    • B01J20/3242—Layers with a functional group, e.g. an affinity material, a ligand, a reactant or a complexing group
    • B01J20/3244—Non-macromolecular compounds
    • B01J20/3246—Non-macromolecular compounds having a well defined chemical structure
    • B01J20/3248—Non-macromolecular compounds having a well defined chemical structure the functional group or the linking, spacer or anchoring group as a whole comprising at least one type of heteroatom selected from a nitrogen, oxygen or sulfur, these atoms not being part of the carrier as such
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
    • B01J20/30—Processes for preparing, regenerating, or reactivating
    • B01J20/32—Impregnating or coating ; Solid sorbent compositions obtained from processes involving impregnating or coating
    • B01J20/3231—Impregnating or coating ; Solid sorbent compositions obtained from processes involving impregnating or coating characterised by the coating or impregnating layer
    • B01J20/3242—Layers with a functional group, e.g. an affinity material, a ligand, a reactant or a complexing group
    • B01J20/3244—Non-macromolecular compounds
    • B01J20/3246—Non-macromolecular compounds having a well defined chemical structure
    • B01J20/3248—Non-macromolecular compounds having a well defined chemical structure the functional group or the linking, spacer or anchoring group as a whole comprising at least one type of heteroatom selected from a nitrogen, oxygen or sulfur, these atoms not being part of the carrier as such
    • B01J20/3251—Non-macromolecular compounds having a well defined chemical structure the functional group or the linking, spacer or anchoring group as a whole comprising at least one type of heteroatom selected from a nitrogen, oxygen or sulfur, these atoms not being part of the carrier as such comprising at least two different types of heteroatoms selected from nitrogen, oxygen or sulphur
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07D—HETEROCYCLIC COMPOUNDS
    • C07D251/00—Heterocyclic compounds containing 1,3,5-triazine rings
    • C07D251/02—Heterocyclic compounds containing 1,3,5-triazine rings not condensed with other rings
    • C07D251/12—Heterocyclic compounds containing 1,3,5-triazine rings not condensed with other rings having three double bonds between ring members or between ring members and non-ring members
    • C07D251/26—Heterocyclic compounds containing 1,3,5-triazine rings not condensed with other rings having three double bonds between ring members or between ring members and non-ring members with only hetero atoms directly attached to ring carbon atoms
    • C07D251/40—Nitrogen atoms
    • C07D251/54—Three nitrogen atoms
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07D—HETEROCYCLIC COMPOUNDS
    • C07D251/00—Heterocyclic compounds containing 1,3,5-triazine rings
    • C07D251/02—Heterocyclic compounds containing 1,3,5-triazine rings not condensed with other rings
    • C07D251/12—Heterocyclic compounds containing 1,3,5-triazine rings not condensed with other rings having three double bonds between ring members or between ring members and non-ring members
    • C07D251/26—Heterocyclic compounds containing 1,3,5-triazine rings not condensed with other rings having three double bonds between ring members or between ring members and non-ring members with only hetero atoms directly attached to ring carbon atoms
    • C07D251/40—Nitrogen atoms
    • C07D251/54—Three nitrogen atoms
    • C07D251/70—Other substituted melamines
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
    • G01N33/6818—Sequencing of polypeptides

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Food Science & Technology (AREA)
  • Cell Biology (AREA)
  • General Physics & Mathematics (AREA)
  • Animal Behavior & Ethology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Epidemiology (AREA)
  • Genetics & Genomics (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Inorganic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Peptides Or Proteins (AREA)
  • Solid-Sorbent Or Filter-Aiding Compositions (AREA)
  • Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)
  • Cosmetics (AREA)
CN201380024731.3A 2012-03-16 2013-03-15 用于蛋白质纯化的亲和试剂 Expired - Fee Related CN104620104B (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201261611999P 2012-03-16 2012-03-16
US61/611,999 2012-03-16
PCT/US2013/032675 WO2013138793A2 (en) 2012-03-16 2013-03-15 Affinity reagants for protein purification

Publications (2)

Publication Number Publication Date
CN104620104A CN104620104A (zh) 2015-05-13
CN104620104B true CN104620104B (zh) 2017-03-01

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Country Status (6)

Country Link
US (2) US9737875B2 (enExample)
EP (1) EP2825694A4 (enExample)
CN (1) CN104620104B (enExample)
HK (1) HK1206420A1 (enExample)
IN (1) IN2014DN08481A (enExample)
WO (1) WO2013138793A2 (enExample)

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Publication number Priority date Publication date Assignee Title
US20140220217A1 (en) 2011-07-12 2014-08-07 Maraxi, Inc. Method and compositions for consumables
PT2731451T (pt) 2011-07-12 2018-11-27 Impossible Foods Inc Métodos e composições para produtos de consumo
US10039306B2 (en) 2012-03-16 2018-08-07 Impossible Foods Inc. Methods and compositions for consumables
BR112014000614A2 (pt) 2011-07-12 2017-07-11 Maraxi Inc métodos e composições para consumíveis
US9737875B2 (en) 2012-03-16 2017-08-22 Impossible Foods Inc. Affinity reagents for protein purification
RU2672489C2 (ru) 2013-01-11 2018-11-15 Импоссибл Фудз Инк. Немолочный аналог сыра, содержащий коацерват
DK2943072T3 (en) 2013-01-11 2018-03-05 Impossible Foods Inc METHODS AND COMPOSITIONS FOR INFLUENCING THE TASTE AND AROMA PROFILE OF CONSUMER PRODUCTS
WO2015042405A1 (en) * 2013-09-19 2015-03-26 Impossible Foods Inc. Ph switchable reagents and methods for their use
BR112016009803B1 (pt) 2013-10-30 2022-02-08 The Curators Of The University Of Missouri Método para especificação e cultivo de linhagem muscular esquelética escalonável
KR20160140790A (ko) 2014-03-31 2016-12-07 임파서블 푸즈 인크. 분쇄 고기 모조물
ES2985769T3 (es) 2017-05-06 2024-11-07 Upside Foods Inc Composiciones y métodos para aumentar la densidad de cultivo de una biomasa celular dentro de una infraestructura de cultivo
WO2019014652A1 (en) 2017-07-13 2019-01-17 Memphis Meats, Inc. COMPOSITIONS AND METHODS FOR INCREASING THE EFFICACY OF CELL CULTURES USED IN FOOD PRODUCTION
CA3076249A1 (en) 2017-09-21 2019-03-28 Dana-Farber Cancer Institute, Inc. Isolation, preservation, compositions and uses of extracts from justicia plants
US11051532B2 (en) 2017-09-22 2021-07-06 Impossible Foods Inc. Methods for purifying protein
CN108176386B (zh) * 2018-01-16 2020-11-06 武汉轻工大学 一种三聚氰胺键合色谱固定相及其制备方法和金属离子改性三聚氰胺键合色谱固定相
WO2021113985A1 (en) * 2019-12-12 2021-06-17 University Of Guelph Plant-based cheese product
IL322673A (en) 2019-12-31 2025-10-01 Air Protein Inc High-protein food compositions
AU2021229443A1 (en) 2020-03-02 2022-09-15 Air Protein, Inc. Structured high-protein meat analogue compositions
US12011016B2 (en) 2020-09-14 2024-06-18 Impossible Foods Inc. Protein methods and compositions
US10894812B1 (en) 2020-09-30 2021-01-19 Alpine Roads, Inc. Recombinant milk proteins
EP4222167A1 (en) 2020-09-30 2023-08-09 Nobell Foods, Inc. Recombinant milk proteins and food compositions comprising the same
US10947552B1 (en) 2020-09-30 2021-03-16 Alpine Roads, Inc. Recombinant fusion proteins for producing milk proteins in plants
US20230380373A1 (en) 2020-10-28 2023-11-30 Pioneer Hi-Bred International, Inc. Leghemoglobin in soybean
CA3231125A1 (en) 2021-10-19 2023-04-27 Joshua MARCH Plant base/animal cell hybrid meat substitute
WO2023200711A1 (en) * 2022-04-11 2023-10-19 Memorial Sloan-Kettering Cancer Center Novel bead link (blink) method for molecular archiving of dna

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US5582988A (en) * 1994-09-15 1996-12-10 Johnson & Johnson Clinical Diagnostics, Inc. Methods for capture and selective release of nucleic acids using weakly basic polymer and amplification of same
US20030166002A1 (en) * 2001-12-12 2003-09-04 Young-Tae Chang Triazine library with linkers
WO2005049653A1 (en) * 2003-07-14 2005-06-02 Genway Biotech, Inc. Affinity separation composition and method
US20080261202A1 (en) * 2002-12-16 2008-10-23 Invitrogen Corporation Tagged Polyfunctional Reagents Capable of Reversibly Binding Target Substances in a pH-dependent Manner
US20090004641A1 (en) * 2002-09-12 2009-01-01 Invitrogen Corporation Site-specific labeling of affinity peptides in fusion proteins
US20100173889A1 (en) * 2008-10-23 2010-07-08 Gruenenthal Gmbh Substituted Pyrimidine and Triazine Compounds
WO2011012302A1 (en) * 2009-07-28 2011-02-03 Instraction Gmbh Specific sorbent for binding proteins and peptides, and separation method using the same

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CA2346989A1 (en) 1998-10-19 2000-04-27 The Board Of Trustees Of The Leland Stanford Junior University Dna-templated combinatorial library chemistry
DE60043397D1 (de) 1999-12-28 2010-01-07 Pharmacopeia Inc Cytokine, insbesondere tnf-alpha, hemmer
US9737875B2 (en) * 2012-03-16 2017-08-22 Impossible Foods Inc. Affinity reagents for protein purification

Patent Citations (7)

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Publication number Priority date Publication date Assignee Title
US5582988A (en) * 1994-09-15 1996-12-10 Johnson & Johnson Clinical Diagnostics, Inc. Methods for capture and selective release of nucleic acids using weakly basic polymer and amplification of same
US20030166002A1 (en) * 2001-12-12 2003-09-04 Young-Tae Chang Triazine library with linkers
US20090004641A1 (en) * 2002-09-12 2009-01-01 Invitrogen Corporation Site-specific labeling of affinity peptides in fusion proteins
US20080261202A1 (en) * 2002-12-16 2008-10-23 Invitrogen Corporation Tagged Polyfunctional Reagents Capable of Reversibly Binding Target Substances in a pH-dependent Manner
WO2005049653A1 (en) * 2003-07-14 2005-06-02 Genway Biotech, Inc. Affinity separation composition and method
US20100173889A1 (en) * 2008-10-23 2010-07-08 Gruenenthal Gmbh Substituted Pyrimidine and Triazine Compounds
WO2011012302A1 (en) * 2009-07-28 2011-02-03 Instraction Gmbh Specific sorbent for binding proteins and peptides, and separation method using the same

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Publication number Publication date
EP2825694A4 (en) 2016-04-20
US9737875B2 (en) 2017-08-22
HK1206420A1 (en) 2016-01-08
WO2013138793A3 (en) 2014-12-11
WO2013138793A2 (en) 2013-09-19
IN2014DN08481A (enExample) 2015-05-08
US20150087532A1 (en) 2015-03-26
US20170320041A1 (en) 2017-11-09
EP2825694A2 (en) 2015-01-21
CN104620104A (zh) 2015-05-13
US9833768B2 (en) 2017-12-05

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