CN104616578B - Production process of sargassum fusiforme specimen - Google Patents
Production process of sargassum fusiforme specimen Download PDFInfo
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- CN104616578B CN104616578B CN201510088113.6A CN201510088113A CN104616578B CN 104616578 B CN104616578 B CN 104616578B CN 201510088113 A CN201510088113 A CN 201510088113A CN 104616578 B CN104616578 B CN 104616578B
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- 241000264279 Sargassum fusiforme Species 0.000 title claims abstract description 32
- 238000004519 manufacturing process Methods 0.000 title abstract 5
- 238000004513 sizing Methods 0.000 claims abstract description 13
- 238000011161 development Methods 0.000 claims abstract description 8
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 7
- 239000003292 glue Substances 0.000 claims abstract description 5
- 239000012535 impurity Substances 0.000 claims abstract description 5
- 238000001035 drying Methods 0.000 claims abstract description 4
- 238000013138 pruning Methods 0.000 claims abstract description 4
- 239000000123 paper Substances 0.000 claims description 23
- 230000002745 absorbent Effects 0.000 claims description 19
- 239000002250 absorbent Substances 0.000 claims description 19
- 238000002360 preparation method Methods 0.000 claims description 9
- 229920000615 alginic acid Polymers 0.000 claims description 8
- 238000005516 engineering process Methods 0.000 claims description 8
- 238000004026 adhesive bonding Methods 0.000 claims description 7
- 239000002390 adhesive tape Substances 0.000 claims description 7
- 230000000877 morphologic effect Effects 0.000 claims description 7
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 claims description 6
- 235000010443 alginic acid Nutrition 0.000 claims description 6
- 230000018044 dehydration Effects 0.000 claims description 6
- 238000006297 dehydration reaction Methods 0.000 claims description 6
- 238000002372 labelling Methods 0.000 claims description 6
- 210000000056 organ Anatomy 0.000 claims description 6
- 238000012545 processing Methods 0.000 claims description 6
- 241000512259 Ascophyllum nodosum Species 0.000 claims description 5
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 claims description 5
- 241000199919 Phaeophyceae Species 0.000 claims description 5
- 241000195493 Cryptophyta Species 0.000 claims description 4
- 239000004816 latex Substances 0.000 claims description 4
- 229920000126 latex Polymers 0.000 claims description 4
- 241001465754 Metazoa Species 0.000 claims description 3
- 229960000583 acetic acid Drugs 0.000 claims description 3
- 238000004140 cleaning Methods 0.000 claims description 3
- 230000006835 compression Effects 0.000 claims description 3
- 238000007906 compression Methods 0.000 claims description 3
- 238000010612 desalination reaction Methods 0.000 claims description 3
- 239000012153 distilled water Substances 0.000 claims description 3
- 239000013505 freshwater Substances 0.000 claims description 3
- 239000012362 glacial acetic acid Substances 0.000 claims description 3
- 238000011084 recovery Methods 0.000 claims description 3
- 239000013535 sea water Substances 0.000 claims description 3
- 238000009423 ventilation Methods 0.000 claims description 2
- 150000004676 glycans Chemical class 0.000 claims 1
- 229920001282 polysaccharide Polymers 0.000 claims 1
- 239000005017 polysaccharide Substances 0.000 claims 1
- 238000011160 research Methods 0.000 abstract description 4
- 239000002932 luster Substances 0.000 abstract description 3
- 238000011033 desalting Methods 0.000 abstract 1
- 238000007493 shaping process Methods 0.000 abstract 1
- 238000005406 washing Methods 0.000 abstract 1
- 230000000366 juvenile effect Effects 0.000 description 4
- 241000196324 Embryophyta Species 0.000 description 3
- 239000002775 capsule Substances 0.000 description 3
- 239000010410 layer Substances 0.000 description 3
- 241000195522 Fucales Species 0.000 description 2
- 241001260375 Hizikia Species 0.000 description 2
- 241000195475 Sargassaceae Species 0.000 description 2
- 229920002472 Starch Polymers 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 2
- 230000010159 dioecy Effects 0.000 description 2
- 210000001161 mammalian embryo Anatomy 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 235000019698 starch Nutrition 0.000 description 2
- 239000008107 starch Substances 0.000 description 2
- 240000007594 Oryza sativa Species 0.000 description 1
- 235000007164 Oryza sativa Nutrition 0.000 description 1
- 241000015177 Saccharina japonica Species 0.000 description 1
- 241001261505 Undaria Species 0.000 description 1
- 239000000853 adhesive Substances 0.000 description 1
- 230000001070 adhesive effect Effects 0.000 description 1
- 239000012141 concentrate Substances 0.000 description 1
- 235000013312 flour Nutrition 0.000 description 1
- 210000004392 genitalia Anatomy 0.000 description 1
- 238000000034 method Methods 0.000 description 1
- 239000002365 multiple layer Substances 0.000 description 1
- 235000009566 rice Nutrition 0.000 description 1
- 150000003839 salts Chemical class 0.000 description 1
- 238000007789 sealing Methods 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 239000004094 surface-active agent Substances 0.000 description 1
- 230000004083 survival effect Effects 0.000 description 1
Classifications
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- G—PHYSICS
- G09—EDUCATION; CRYPTOGRAPHY; DISPLAY; ADVERTISING; SEALS
- G09B—EDUCATIONAL OR DEMONSTRATION APPLIANCES; APPLIANCES FOR TEACHING, OR COMMUNICATING WITH, THE BLIND, DEAF OR MUTE; MODELS; PLANETARIA; GLOBES; MAPS; DIAGRAMS
- G09B23/00—Models for scientific, medical, or mathematical purposes, e.g. full-sized devices for demonstration purposes
- G09B23/38—Models for scientific, medical, or mathematical purposes, e.g. full-sized devices for demonstration purposes for botany
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- Physics & Mathematics (AREA)
- Engineering & Computer Science (AREA)
- General Physics & Mathematics (AREA)
- Mathematical Analysis (AREA)
- Mathematical Physics (AREA)
- Algebra (AREA)
- Computational Mathematics (AREA)
- Botany (AREA)
- Health & Medical Sciences (AREA)
- Mathematical Optimization (AREA)
- General Health & Medical Sciences (AREA)
- Pure & Applied Mathematics (AREA)
- Business, Economics & Management (AREA)
- Life Sciences & Earth Sciences (AREA)
- Educational Administration (AREA)
- Educational Technology (AREA)
- Theoretical Computer Science (AREA)
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Abstract
The invention provides a production process of a sargassum fusiforme specimen. The production process is characterized by comprising the following steps: selecting sargassum fusiforme; removing impurities and desalting; desugaring; washing; dehydrating; expanding braches and pruning; fixing the shape; flattening and sucking water; drying in the shade and shaping; sticking with glue and fixing; sizing; marking; and mounting. Aiming at finishing the production of specimens with different strain characteristics and life history development phases of the sargassum fusiforme, the invention provides the production process of the sargassum fusiforme specimen; the sargassum fusiforme specimen can keep original growing shape and color and luster, is easy to store for a long period and is good for scientific researches and practical teaching.
Description
Technical field
The present invention relates to a kind of processing technology of Sargassum fusiforme (Harv.) Setch specimen.
Background technology
Sargassum fusiforme (Harv.) Setch (Hizikia fusiforme (Harv.) Seteh. or Hizikia fusiformis (Hary.)
Okam.) Phaeophyta (Phaeophyta), dumpling made of glutinous rice flour guiding principle (Cyclospreae), Fucales (Fucales), Sargassaceae are belonged to
(Sargassaceae), Hizikia (Hizikia), is the distinctive perennial kelp of Pacific Northwest immediate offshore area.
Sargassum fusiforme (Harv.) Setch population is distributed mainly on China (north to Liaodong Peninsula south is to the Guangdong Lezhou Peninsula), Japanese (Hokkaido south Jing Honshu
To nine divisions of China in remote antiquity) and the Korea peninsula (eastern bank, southern bank and southwestern bank) immediate offshore area.China Liaoning, Shandong, Zhejiang, Coastal Area of Fujian Surveyed
Widely it is distributed, especially best with Zhejiang, the growth of Fujian coastal area, yield is high, best in quality.Sargassum fusiforme (Harv.) Setch dioecism, according to its life
The history stage of development living, it is followed successively by：Gamete, embryo, juvenile sporophyte, sporinite and ripe sporinite.Wherein, gamete and embryo period, by
It is less in its build, the making of specimen is not suitable as, in juvenile sporophyte and its sporinite in later stage and ripe sporinite period
The making of suitable specimen.
, in juvenile sporophyte period, based on lanceolar or rod, sporinite period leaf specialization is into spindle, circle for leaf for Sargassum fusiforme (Harv.) Setch
The cluster anger capsule of cylindricality or spherical balloon, at air bag cluster life, has 1～2 feature air vesicle, air bag tool suspensor, utricule and capsule point
(indivedual strains without capsule point) three parts, ripe sporinite intact form include cylindrical rhizoid, cylindrical stem, lanceolate leaf (or
Polymorphic hollow gasbag) and the cylindrical part of receptacle four.Two kinds of life cycle of having property of Sargassum fusiforme (Harv.) Setch and asexual propagation, in large-scale sea
Belong to high type in the natural evolution system of algae, substantially, dioecism, genitals are receptacle to sporophyte generation.Frond
It is made up of rhizoid, stem, blade and air bag.Rhizoid for sucker shape base portion holdfast, stem for right circular cylinder shape major branch, blade,
Air bag, the north is serrated；It is southern then in linear or bar-shaped.
Due to Sargassum fusiforme (Harv.) Setch morphological characteristic and Thallus Laminariae (Thallus Eckloniae) (Laminaria japonica) and Thallus Laminariae (Undaria
The kelp morphological characteristic such as pinnatifida) differs greatly, therefore, its preparation of specimen and larger, the Shang Shu states that preserve difficulty
Interior, international blank, the present invention is differentiated and scientific research and institution of higher learning plant based on Sargassum fusiforme (Harv.) Setch preserving seed, strain morphological differencess
Subject practical teaching needs, and Jing years'experiences summarize Sargassum fusiforme (Harv.) Setch preparation of specimen technique.
The content of the invention
Based on the problems referred to above, present invention aim at providing a kind of processing technology of Sargassum fusiforme (Harv.) Setch specimen, the Sargassum fusiforme (Harv.) Setch specimen
Its script growthform and color and luster can be kept, can be preserved for a long time, beneficial to scientific research and practical teaching.
For problem above, there is provided following technical scheme：A kind of processing technology of Sargassum fusiforme (Harv.) Setch specimen, it is characterised in that bag
Include following steps：
A, dish is selected, according to Sargassum fusiforme (Harv.) Setch strain feature and life cycle stage of development, chosen corresponding with its period stage of development
Feature is obvious, the Fresh Plants that form is complete, and 4～8 DEG C of deepfreezes, lucifuges are transported to laboratory；After being placed in laboratory, Jing 3～5
Hour slow recovery temperature carefully takes out fresh sample to 23 DEG C ± 2 DEG C, puts salinity for 33 ‰～35 ‰, and pH value is 7.8～
In 8.3 sea water, each sample is set fully to unfold so as to keep vigorous growth state；
B, remove impurity, desalination, remove macroscopic other periphtic algaes and animal, and with fresh water 3～5min is slowly rinsed, and take off
Remove salt surfactant；
C, desaccharide, sample sets to 0 and embathe in .01mol/L hydrochloric acid or glacial acetic acid 4～5min to remove kelp surface distinctive
Brown algae sulfated polysaccharide-Algin；
D, cleaning, sample is put in distilled water and is cleaned multiple times, until thoroughly removing its remained on surface Algin and salinity；
E, dehydration, flatten sample, with its excess surface moisture of absorbent paper gentle aspiration at flat board；
F, Zhan Zhi, pruning, put absorbent paper surface that is fixed, paving, by the histoorgan of sample than comparatively dense place by sample
Carry out selective removal, the obvious histoorgan of keeping characteristics；
G, form are fixed, and are fixed on each histoorgan is smooth in absorbent paper using adhesive tape, and keep projecting each portion
The form of feature；
H, water suction is flattened, according to sample size after fixation, select the specimen holder of size appropriateness, and sample is put completed in advance
On multi-layer absorbent paper, multi-layer absorbent paper is placed again on top；
I, setting of drying in the shade, compression, fixed preparation folder, put room temperature, lucifuge, ventilation, and an absorbent paper was changed per 3～4 days,
To the abundant dehydration sizing of specimen；
J, gluing, fixed, sample after taking-up sizing, remove adhesive tape, it is to avoid organ comes off, and keep sample integrity；
By sample one side, latex is smeared uniformly, in right amount, keep fully projecting under the premise of each portion's morphological characteristic, progressively paste and put in advance
On ready specimen cardboard；
K, sizing, the sample after gluing fixation is put in the specimen holder of prior laid absorbent paper, and sizing fixes 3～5 days, extremely
Glue is dry and sample is fixed on specimen cardboard, takes out fixed sample；
L, labelling, fix cardboard and select appropriately sized label paper according to specimen, make the label of the specimen；
M, mounting.
Specific embodiment
With reference to embodiment, the specific embodiment of the present invention is described in further detail.Following examples are used for
The present invention is illustrated, but is not limited to the scope of the present invention.
A kind of processing technology of Sargassum fusiforme (Harv.) Setch specimen, the object of the preparation of specimen in the present embodiment is ripe sporinite period
Sargassum fusiforme (Harv.) Setch, it has rhizoid, leaf, stem and receptacle, it is characterised in that comprise the steps：
A, dish is selected, according to Sargassum fusiforme (Harv.) Setch strain feature and life cycle stage of development, choose rhizoid, stem, leaf and receptacle feature
Significantly, the complete Fresh Plants of form, 4～8 DEG C of deepfreezes, lucifuges are transported to laboratory；After being placed in laboratory, Jing 3～5 is little
When slow recovery temperature to 23 DEG C ± 2 DEG C, 23 DEG C of optimal life temperatures for Sargassum fusiforme (Harv.) Setch ensure that the survival of Sargassum fusiforme (Harv.) Setch, little
The heart takes out fresh sample, and it is 33 ‰～35 ‰ to put salinity, and pH value is in 7.8～8.3 sea water, sample is fully unfolded, and is kept
Its vigorous growth state；
B, remove impurity, desalination, it is manual to remove the magazines such as macroscopic other periphtic algaes and animal, slowly rinsed with fresh water
3～5min, sloughs sample surface salinity；This step can be prevented after preparation of specimen, and its surface has impurity and separates out white
Salinity.
C, desaccharide, sample sets to 0 and embathe in .01mol/L dilute hydrochloric acid or glacial acetic acid 4～5min to remove kelp surface peculiar
Brown algae sulfated polysaccharide-Algin；Because Algin contains sugar, the glue-line is removed in this step, its surface can be facilitated to enter
Row gluing, because latex contains starch, starch belongs to macromolecular structure with sugar, and both are immiscible, therefore needs to remove in this step
The Algin.
D, cleaning, sample is put in distilled water and is cleaned multiple times, until thoroughly removing its remained on surface Algin and salinity；
E, dehydration, flatten sample, with its excess surface moisture of absorbent paper gentle aspiration at flat board；
F, Zhan Zhi, pruning, by sample absorbent paper that is fixed, paving or newspaper surface are put, with dissecting needle and tweezers by frond
The organs such as branch, leaf and receptacle are sufficiently spread out, and side life stem, leaf and receptacle are selected than comparatively dense place with dissecting knife or dissecting scissorss
Removal is selected, the obvious histoorgan of keeping characteristics is unlikely to concentrate very much；
G, form are fixed, and using adhesive tape by the smooth fixation of rhizoid, stem, leaf and receptacle, and keep projecting each portion spy
The form levied；
H, water suction is flattened, according to sample size after fixation, select the specimen holder of size appropriateness, and sample is put completed in advance
On multi-layer absorbent paper, multilamellar newspaper is placed again on top, and this step can carry out multiple-layer stacked；
I, setting of drying in the shade, compression, fixed preparation folder are put room temperature (room temperature at this place refers to conventional chambers temperature), lucifuge, are led to
At wind, an absorbent paper was changed per 3～4 days, to the abundant dehydration sizing of specimen, meanwhile, sample can for a long time be preserved in this stepping row.
J, gluing, fixed, sample after taking-up sizing, remove adhesive tape, it is to avoid organ comes off, and keep specimen integrity,
If causing to come off when indivedual leaves or receptacle are because of artificial removal adhesive tape, need labelling to come off place and correspondence comes off organ；By sample one
Side, smears uniformly, in right amount latex, keeps fully projecting under the premise of each portion's morphological characteristic, progressively pastes and puts preprepared
On specimen cardboard；
K, sizing, the sample after gluing fixation is put in the specimen holder of prior laid absorbent paper, and sizing fixes 3～5 days, extremely
After set time, treat that glue is dry and sample is fixed on specimen holder, take out fixed sample, go through fixing situation, if occurring dredging
The solid organ of leak adhesive, can be fixed with dissecting needle from new glue；
L, labelling, fix cardboard and select appropriately sized label paper, detailed labelling specimen Chinese, Latin according to specimen
The information such as title, acquisition time, collecting location, Life Stages, main morphological features and life habit, by the mark of record information
Sign paper stickup and put bottom at specimen morphology rhizoid, blank space center position or bottom right position；
M, mounting, select the appropriate specimen mounting frame of size, and the specimen plate of labelling Sargassum fusiforme (Harv.) Setch specimen is loaded wherein, and back adds
Plus splash guard, rear sealing specimen.
Above step is equally applicable to carry out preparation of specimen to the Sargassum fusiforme (Harv.) Setch in juvenile sporophyte and sporinite period, through making
Sargassum fusiforme (Harv.) Setch specimen afterwards can keep its script growthform and color and luster according to different strain features and life cycle stage of development,
Beneficial to scientific research and practical teaching.
The above is only the preferred embodiment of the present invention, it is noted that for the ordinary skill people of the art
For member, on the premise of without departing from the technology of the present invention principle, some improvement and modification can also be made, above-mentioned hypothesis these
Improve and modification also should be regarded as protection scope of the present invention.
Claims (1)
1. a kind of processing technology of Sargassum fusiforme (Harv.) Setch specimen, it is characterised in that comprise the steps：
A, dish is selected, according to Sargassum fusiforme (Harv.) Setch strain feature and life cycle stage of development, choose feature corresponding with its period stage of development
Substantially, the complete Fresh Plants of form, 4～8 DEG C of deepfreezes, lucifuges are transported to laboratory；After being placed in laboratory, Jing 3～5 hours
Slow recovery temperature carefully takes out fresh sample to 23 DEG C ± 2 DEG C, and it is 33 ‰～35 ‰ to put salinity, and pH value is 7.8～8.3
In sea water, each sample is set fully to unfold so as to keep vigorous growth state；
B, remove impurity, desalination, remove macroscopic other periphtic algaes and animal, and with fresh water 3～5min is slowly rinsed, and slough table
Face salinity；
C, desaccharide, sample sets to 0 and embathe in .01mol/L hydrochloric acid or glacial acetic acid the distinctive Brown algae in 4～5min removal kelps surface
The Algin of sulfated polysaccharide one；
D, cleaning, sample is put in distilled water and is cleaned multiple times, until thoroughly removing its remained on surface Algin and salinity；
E, dehydration, flatten sample, with its excess surface moisture of absorbent paper gentle aspiration at flat board；
F, Zhan Zhi, pruning, by sample absorbent paper surface that is fixed, paving is put, and the histoorgan of sample is carried out than comparatively dense place
Selective removal, the obvious histoorgan of keeping characteristics；
G, form are fixed, and are fixed on each histoorgan is smooth in absorbent paper using adhesive tape, and keep projecting each portion's feature
Form；
H, water suction is flattened, according to sample size after fixation, select the specimen holder of size appropriateness, and sample is put complete in advance multilamellar
In absorbent paper, multi-layer absorbent paper is placed again on top；
L, setting of drying in the shade, compression, fixed preparation folder, put room temperature, lucifuge, ventilation, an absorbent paper are changed per 3～4 days, to mark
This fully dehydration sizing；
J, gluing, fixed, sample after taking-up sizing, remove adhesive tape, it is to avoid organ comes off, and keep sample integrity；By sample
This one side, smears uniformly, in right amount latex, keeps fully projecting under the premise of each portion's morphological characteristic, progressively pastes and puts prior preparation
On good specimen cardboard；
K, sizing, the sample after gluing fixation is put in the specimen holder of prior laid absorbent paper, and sizing fixes 3～5 days, dry to glue
And sample is fixed on specimen cardboard, fixed sample is taken out；
L, labelling, fix cardboard and select appropriately sized label paper according to specimen, make the label of the specimen；
M, mounting.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201510088113.6A CN104616578B (en) | 2015-02-12 | 2015-02-12 | Production process of sargassum fusiforme specimen |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201510088113.6A CN104616578B (en) | 2015-02-12 | 2015-02-12 | Production process of sargassum fusiforme specimen |
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| CN104616578A CN104616578A (en) | 2015-05-13 |
| CN104616578B true CN104616578B (en) | 2017-04-12 |
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| CN201510088113.6A Expired - Fee Related CN104616578B (en) | 2015-02-12 | 2015-02-12 | Production process of sargassum fusiforme specimen |
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Families Citing this family (5)
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| CN108353892A (en) * | 2018-01-05 | 2018-08-03 | 湖南农业大学 | The production method of rice plant sample |
| CN108925551A (en) * | 2018-07-09 | 2018-12-04 | 公安县中医医院 | A kind of method of microwave production plant specimen |
| CN109258629A (en) * | 2018-11-07 | 2019-01-25 | 滁州学院 | Waxed plant leaves Slide processing |
| CN109693486A (en) * | 2019-01-30 | 2019-04-30 | 云南一木生态文化传播有限公司 | A kind of production method of Leaf shape vein science and education craftwork |
| CN112450207B (en) * | 2020-11-26 | 2022-02-18 | 德江县绿通天麻发展有限公司 | Method for preparing rhizoma gastrodiae specimen |
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| JPS5249129A (en) * | 1975-10-17 | 1977-04-19 | Akio Imuro | Method of dyeing transparent resin packaging plant sample |
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| CN1045476A (en) * | 1989-03-10 | 1990-09-19 | 姜铁容 | A kind of method of manufacturing sample |
| JPH0892002A (en) * | 1994-09-19 | 1996-04-09 | Plan Tooku:Kk | Sample for display |
| JPH11240801A (en) * | 1998-02-23 | 1999-09-07 | Akio Imuro | Production of dried plant specimen |
| CN101458182A (en) * | 2008-12-22 | 2009-06-17 | 华中科技大学 | Method for producing brain sample of small animal |
| CN101548670A (en) * | 2009-04-21 | 2009-10-07 | 宁波大学 | Method for praparing varek specimen |
| CN102524245A (en) * | 2011-12-15 | 2012-07-04 | 河南科技大学 | Preparation method of plant unbleached impregnated specimen |
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2015
- 2015-02-12 CN CN201510088113.6A patent/CN104616578B/en not_active Expired - Fee Related
Patent Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS5249129A (en) * | 1975-10-17 | 1977-04-19 | Akio Imuro | Method of dyeing transparent resin packaging plant sample |
| JPS6470403A (en) * | 1987-09-10 | 1989-03-15 | Yoshio Sugino | Preparation of pressed flower |
| CN1045476A (en) * | 1989-03-10 | 1990-09-19 | 姜铁容 | A kind of method of manufacturing sample |
| JPH0892002A (en) * | 1994-09-19 | 1996-04-09 | Plan Tooku:Kk | Sample for display |
| JPH11240801A (en) * | 1998-02-23 | 1999-09-07 | Akio Imuro | Production of dried plant specimen |
| CN101458182A (en) * | 2008-12-22 | 2009-06-17 | 华中科技大学 | Method for producing brain sample of small animal |
| CN101548670A (en) * | 2009-04-21 | 2009-10-07 | 宁波大学 | Method for praparing varek specimen |
| CN102524245A (en) * | 2011-12-15 | 2012-07-04 | 河南科技大学 | Preparation method of plant unbleached impregnated specimen |
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| CN104616578A (en) | 2015-05-13 |
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