CN105941141A - Ploidy mutagenesis method of brier grape buds covered by propyzamide - Google Patents
Ploidy mutagenesis method of brier grape buds covered by propyzamide Download PDFInfo
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- CN105941141A CN105941141A CN201610375056.4A CN201610375056A CN105941141A CN 105941141 A CN105941141 A CN 105941141A CN 201610375056 A CN201610375056 A CN 201610375056A CN 105941141 A CN105941141 A CN 105941141A
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- 238000000034 method Methods 0.000 title claims abstract description 35
- 231100000350 mutagenesis Toxicity 0.000 title claims abstract description 19
- 238000002703 mutagenesis Methods 0.000 title claims abstract description 14
- PHNUZKMIPFFYSO-UHFFFAOYSA-N propyzamide Chemical compound C#CC(C)(C)NC(=O)C1=CC(Cl)=CC(Cl)=C1 PHNUZKMIPFFYSO-UHFFFAOYSA-N 0.000 title abstract description 14
- 239000005602 Propyzamide Substances 0.000 title abstract description 12
- 235000009754 Vitis X bourquina Nutrition 0.000 title abstract description 10
- 235000012333 Vitis X labruscana Nutrition 0.000 title abstract description 10
- 235000014787 Vitis vinifera Nutrition 0.000 title abstract description 10
- 240000006365 Vitis vinifera Species 0.000 title 1
- 241000219095 Vitis Species 0.000 claims abstract description 73
- 229920001817 Agar Polymers 0.000 claims abstract description 15
- 239000008272 agar Substances 0.000 claims abstract description 15
- IAZDPXIOMUYVGZ-UHFFFAOYSA-N Dimethylsulphoxide Chemical compound CS(C)=O IAZDPXIOMUYVGZ-UHFFFAOYSA-N 0.000 claims abstract description 14
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 12
- 235000009392 Vitis Nutrition 0.000 claims description 64
- QWENMOXLTHDKDL-UHFFFAOYSA-M pentoxymethanedithioate Chemical compound CCCCCOC([S-])=S QWENMOXLTHDKDL-UHFFFAOYSA-M 0.000 claims description 53
- 239000006071 cream Substances 0.000 claims description 11
- 230000035772 mutation Effects 0.000 claims description 5
- 239000002904 solvent Substances 0.000 claims description 3
- 230000012010 growth Effects 0.000 abstract description 9
- 208000035199 Tetraploidy Diseases 0.000 abstract description 5
- 230000000694 effects Effects 0.000 abstract description 4
- 230000034303 cell budding Effects 0.000 abstract 2
- 239000003153 chemical reaction reagent Substances 0.000 abstract 1
- 238000007865 diluting Methods 0.000 abstract 1
- 235000013399 edible fruits Nutrition 0.000 description 13
- IAKHMKGGTNLKSZ-INIZCTEOSA-N (S)-colchicine Chemical compound C1([C@@H](NC(C)=O)CC2)=CC(=O)C(OC)=CC=C1C1=C2C=C(OC)C(OC)=C1OC IAKHMKGGTNLKSZ-INIZCTEOSA-N 0.000 description 12
- 239000000243 solution Substances 0.000 description 12
- 210000000349 chromosome Anatomy 0.000 description 9
- 241000196324 Embryophyta Species 0.000 description 8
- 230000003505 mutagenic effect Effects 0.000 description 8
- 206010028400 Mutagenic effect Diseases 0.000 description 7
- 231100000243 mutagenic effect Toxicity 0.000 description 7
- IBXNCJKFFQIKKY-UHFFFAOYSA-N 1-pentyne Chemical compound CCCC#C IBXNCJKFFQIKKY-UHFFFAOYSA-N 0.000 description 6
- 229960001338 colchicine Drugs 0.000 description 6
- 244000025254 Cannabis sativa Species 0.000 description 5
- 239000011148 porous material Substances 0.000 description 5
- 150000001412 amines Chemical class 0.000 description 4
- 239000002775 capsule Substances 0.000 description 4
- 230000035784 germination Effects 0.000 description 4
- 239000007787 solid Substances 0.000 description 4
- 239000000463 material Substances 0.000 description 3
- 238000012360 testing method Methods 0.000 description 3
- 102000029749 Microtubule Human genes 0.000 description 2
- 108091022875 Microtubule Proteins 0.000 description 2
- 208000020584 Polyploidy Diseases 0.000 description 2
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- 230000009286 beneficial effect Effects 0.000 description 2
- 238000004043 dyeing Methods 0.000 description 2
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- URCHNZQAFSOGNB-UHFFFAOYSA-N 3-[(3,4-dichlorophenyl)methylamino]cyclopent-2-en-1-one Chemical compound C1=C(Cl)C(Cl)=CC=C1CNC1=CC(=O)CC1 URCHNZQAFSOGNB-UHFFFAOYSA-N 0.000 description 1
- 241000238876 Acari Species 0.000 description 1
- 241000193738 Bacillus anthracis Species 0.000 description 1
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 1
- 241001464837 Viridiplantae Species 0.000 description 1
- 244000068653 Vitis davidii Species 0.000 description 1
- FJWGYAHXMCUOOM-QHOUIDNNSA-N [(2s,3r,4s,5r,6r)-2-[(2r,3r,4s,5r,6s)-4,5-dinitrooxy-2-(nitrooxymethyl)-6-[(2r,3r,4s,5r,6s)-4,5,6-trinitrooxy-2-(nitrooxymethyl)oxan-3-yl]oxyoxan-3-yl]oxy-3,5-dinitrooxy-6-(nitrooxymethyl)oxan-4-yl] nitrate Chemical compound O([C@@H]1O[C@@H]([C@H]([C@H](O[N+]([O-])=O)[C@H]1O[N+]([O-])=O)O[C@H]1[C@@H]([C@@H](O[N+]([O-])=O)[C@H](O[N+]([O-])=O)[C@@H](CO[N+]([O-])=O)O1)O[N+]([O-])=O)CO[N+](=O)[O-])[C@@H]1[C@@H](CO[N+]([O-])=O)O[C@@H](O[N+]([O-])=O)[C@H](O[N+]([O-])=O)[C@H]1O[N+]([O-])=O FJWGYAHXMCUOOM-QHOUIDNNSA-N 0.000 description 1
- 239000002390 adhesive tape Substances 0.000 description 1
- 239000003513 alkali Substances 0.000 description 1
- 229930013930 alkaloid Natural products 0.000 description 1
- 150000003797 alkaloid derivatives Chemical class 0.000 description 1
- 150000001345 alkine derivatives Chemical class 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 238000009395 breeding Methods 0.000 description 1
- 230000001488 breeding effect Effects 0.000 description 1
- 210000004027 cell Anatomy 0.000 description 1
- 230000032823 cell division Effects 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 230000003247 decreasing effect Effects 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 230000005662 electromechanics Effects 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 238000000605 extraction Methods 0.000 description 1
- 230000004345 fruit ripening Effects 0.000 description 1
- 239000008103 glucose Substances 0.000 description 1
- 239000003292 glue Substances 0.000 description 1
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- 239000007952 growth promoter Substances 0.000 description 1
- 230000002363 herbicidal effect Effects 0.000 description 1
- 239000004009 herbicide Substances 0.000 description 1
- 230000006698 induction Effects 0.000 description 1
- 230000008595 infiltration Effects 0.000 description 1
- 238000001764 infiltration Methods 0.000 description 1
- 239000010985 leather Substances 0.000 description 1
- 238000012067 mathematical method Methods 0.000 description 1
- 230000035800 maturation Effects 0.000 description 1
- 230000007721 medicinal effect Effects 0.000 description 1
- 239000010413 mother solution Substances 0.000 description 1
- 231100000219 mutagenic Toxicity 0.000 description 1
- 239000003471 mutagenic agent Substances 0.000 description 1
- 229920001220 nitrocellulos Polymers 0.000 description 1
- 229940079938 nitrocellulose Drugs 0.000 description 1
- 230000003204 osmotic effect Effects 0.000 description 1
- 238000000643 oven drying Methods 0.000 description 1
- 125000003538 pentan-3-yl group Chemical group [H]C([H])([H])C([H])([H])C([H])(*)C([H])([H])C([H])([H])[H] 0.000 description 1
- 230000008635 plant growth Effects 0.000 description 1
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- 230000001988 toxicity Effects 0.000 description 1
- 239000012991 xanthate Substances 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/06—Processes for producing mutations, e.g. treatment with chemicals or with radiation
- A01H1/08—Methods for producing changes in chromosome number
Landscapes
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Life Sciences & Earth Sciences (AREA)
- Molecular Biology (AREA)
- General Health & Medical Sciences (AREA)
- Botany (AREA)
- Developmental Biology & Embryology (AREA)
- Environmental Sciences (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
Abstract
The invention discloses a ploidy mutagenesis method of brier grape buds covered by propyzamide. The method comprises the following steps: (1) dissolving propyzamide in dimethyl sulfoxide, and diluting with water till the mass concentration of the propyzamide is 0.005%-0.007% to obtain a propyzamide solution; and (2) preparing the propyzamide solution into propyzamide agar paste, covering the brier grape buds in the budding growth state with the propyzamide agar past for 5-7 days to complete ploidy mutagenesis of the brier grape buds. Through a covering method, the ploidy mutagenesis method induces the brier grape buds in the budding growth state to successfully obtain brier grape tetraploids; the propyzamide is used as a main reagent; when the concentration of the propyzamide solution applied to the brier grape buds is 0.005%, an optimal mutagenesis effect is achieved; the mutagenesis rate reaches 60% or above.
Description
Technical field
The present invention relates to the ploidy method of mutagenesis of a kind of pentyl xanthate shroud Vitis davidi bud.
Background technology
Vitis davidi (V.davidii.Foex) belongs to East Asia population, 2n=2x=38, be south China main wild Fructus Vitis viniferae it
One, it is mainly distributed on the Changjiang river with in south subtropics warm and humid rainforest unbroken mountains, can preferably adapt to high temperature and humidity weather conditions, normal to Fructus Vitis viniferae
Ill harmful white rot, anthrax, anthrachose of grape etc. have fine resistance, and strong adaptability, this distinct performance is that south grape is without public affairs
Evilization plantation depicts bright prospects, also provides outstanding resource and material for the aspect such as Fructus Vitis viniferae moisture-proof stock and breeding of new variety
Material.Vitis davidi the most climing, tender leaf is the abundantest with fruit color, appreciation effect is higher with value ratio common glucose, is park, front yard
Institute etc. good vertical views and admires green plants.The color of the leather of most of Vitis davidi fruit grain is blue black or blue purple, medicinal effects with
The general Fructus Vitis viniferae of value ratio is more notable, is more beneficial for people healthy.Compared with other Fructus Vitis viniferae cultivated on the south the Changjiang river, sting Portugal
Grape are more suitable for processing and the wine brewing of Fructus Vitis viniferae, and its addition product is more rich, and added value is higher.Because of Vitis davidi, to have above-mentioned several respects only
Special performance, on the south the Changjiang river, many local peasants are wild Vitis davidi man's plant and obtain howling success.
Vitis davidi fruit grain is less, and it is more that fruit includes seed.Utilized Colchicine process Vitis davidi Germinating Seeds and
Growth bud, lures its chromosome doubling to be successfully obtained the research report of Vitis davidi polyploid.But Colchicine is a severe toxicity plant
Alkali, careless slightly in operating process, i.e. process object and operator are brought high risks.
Currently there are no and pentyl xanthate is applied to Vitis davidi and induces the reported success of Vitis davidi chromosome doubling, originally grind
The meaning studied carefully is to utilize pentyl xanthate (Propyzamid) to act on first be in the Vitis davidi bud of growth conditions and enter it
Row ploidy is induced, for obtaining the big high-quality of grain, the Vitis davidi New MS Polyploid Variety of few seed or no seed provide excellent germ plasm resource with
New lines.
Summary of the invention
Present invention solves the technical problem that and be, pentyl xanthate is applied to the ploidy mutation of Vitis davidi bud, and explores suitably
Ploidy mutagenic condition.
The technical scheme is that, it is provided that the ploidy method of mutagenesis of a kind of pentyl xanthate shroud Vitis davidi bud, including with
Lower step:
(1) pentyl xanthate is dissolved in dimethyl sulfoxide solvent, then is diluted with water to the mass concentration of pentyl xanthate
0.003%-0.007%, it is thus achieved that pentyl xanthate solution；
(2) described pentyl xanthate solution being made pentyl xanthate agar cream, set covers in the Vitis davidi being in bud
On bud 5-7 days, i.e. complete the ploidy mutation of Vitis davidi bud.
Further, in described pentyl xanthate solution, the mass concentration of dimethyl sulfoxide is 0.04%-0.08%.
Further, under conditions of temperature is 13-18 DEG C, described pentyl xanthate agar cream set is covered on Vitis davidi bud.
Further, in described pentyl xanthate solution, the mass concentration of the suitableeest pentyl xanthate is 0.005%.
Further, the described shroud time is 6 days.
Pentyl xanthate (Propyzamide, CAS:23950-58-5, molecular formula: C12H11Cl2NO；Molecular weight 256.13) again
Cry and oppose Ticks soon；Pronamide；Pentyl xanthate；Propyzamide；Pentyl xanthate standard substance；Pentyl xanthate standard solution etc..It belongs to removes
The one of grass agent, has the strongest adhesion to Microtubules in plants albumen, can effectively stop the polymerization of Microtubules in plants, induces plant dyeing
Body doubles.Compared with Colchicine, pentyl xanthate has hypotoxicity, low concentration, low cost, high efficiency induced chromosome double
Feature, the current market price is 1g/ unit.
The main innovation of the present invention is: 1, the present invention utilizes shroud method, uses pentyl xanthate solution to process Vitis davidi bud,
Successfully Vitis davidi bud is achieved ploidy mutation.Pentyl xanthate solution concentration is very big on the impact of Vitis davidi bud induced mutation rate, this
Inventing the suitable pentyl xanthate solution concentration going out to induce Vitis davidi bud generation ploidy to make a variation by experimental exploring is 0.003%-
0.007% (concentration in the present invention refers both to mass concentration), optium concentration is 0.005%, and the optimal shroud time is 6 days；This use
Amount compared with the consumption of Colchicine, concentration is low more than 100 times.2, the cell division of the Vitis davidi bud of bud it is in
Vigorous, the present invention carries out shroud in this stage, and by the conservative control shroud time, the probability of chromosome doubling improves so that
Vitis davidi bud successfully occurs ploidy to make a variation.3, the solvent of the faint yellow crystalline powder of pentyl xanthate is made of dimethyl sulfoxide (DMSO),
The osmosis to Vitis davidi bud of the pentyl xanthate solution can be strengthened, can realize relatively under the conditions of low pentyl xanthate solution concentration
Good infiltration, improve chromosome Mutagenic Effect in Vitis davidi sprout cell, it is to avoid Vitis davidi bud is permeated by higher drug concentration
Effect impact, reduce Fructus Vitis viniferae bud mortality rate (Vitis davidi bud with 0.005% pentyl xanthate agar cream shroud 6d, rudiment
Rate is still up to 66.7%).
The invention has the beneficial effects as follows, the present invention successfully induces Vitis davidi bud to obtain Vitis davidi four times by shroud method
Body, the pentyl xanthate agar cream that shroud uses, most suitable concentration is about 0.005%, and induced mutation rate is up to more than 60%.Mesh
Before, pentyl xanthate generally makees a kind of herbicide on producing and is using, and Colchicine is a kind of extraction from liliaceous plant
Hypertoxic plant alkaloid, so no matter in terms of the several respects such as concentration, toxicity, cost, induced efficiency height, plant ploidy induction experiment
In if utilize more pentyl xanthate substitute Colchicine make mutagenic agent, low concentration, hypotoxicity, low cost, high induced efficiency
Advantage will be apparent upon.
Accompanying drawing explanation
Fig. 1 represents Vitis davidi diploid chromosome (2n=2x=38) (× 5500)；
Wherein, "×" represents that microscope amplifies figure place；
Fig. 2 represents Vitis davidi tetraploid chromosomes (2n → 4x=76) (× 5500)；
Fig. 3 represents comparison (2n) Vitis davidi pore (× 800)；
Fig. 4 represents variant (4n) Vitis davidi pore (× 800).
Detailed description of the invention
Below in conjunction with embodiment, the invention will be further described.
Embodiment 1
Examination material and the preparation of pentyl xanthate agar cream
On March 21st, 2014, choose from Vitis davidi plant growth roughly the same, have 15 buds (many lateral buds are erased),
And the branches and tendrils being active 12, and the branches and tendrils chosen are evened up bondage.
The preparation of pentyl xanthate agar cream (culture medium): pentyl xanthate is configured to the mother solution that concentration is 0.05%, divides afterwards
Xi Shi not be made into the pentyl xanthate solution that concentration is 0.003%, 0.004%, 0.005%, 0.006%, 0.007%.By agar
(10%) fully it is dissolved in above-mentioned each pentyl xanthate solution and clear water, is respectively prepared each concentration pentyne contained with waterproof capsule
Grass amine agar cream compares agar cream with clear water.
Processing method
This test is carried out with shroud method.With clear water agar cream shroud for comparison, 3 process；Often process shroud to show substantially
Upper bud 2 × 15=30 of identical Vitis davidi 2 branches and tendrils, and with the waterproof capsule of black immobilization with adhesive tape.
After shroud 6d, remove capsule, clean the agar adhered on bud and branches and tendrils with clear water, after send out along with the growth of branches and tendrils
Educating, carefully each histoorgan growth promoter situations such as the branches and tendrils blade that above bud goes out, branches and tendrils, fruit that process of observation are imitated with mutation
Should.
Observation is sent out with the most climing the taking out of Vitis davidi and is carried out with growth.
Each germination rate statistical method that processes:
Germination rate=rudiment number/process bud number (30) × 100%
Each induced mutation rate statistical method that processes:
The variation branches and tendrils number of induced mutation rate=generation/microscopy branches and tendrils number × 100%.
Leaf morphology and water content observation procedure
Gather respectively and stop growing, there is obvious variation features blade (2n → 4x=76) and compare blade (2n=2x
=38) each 50, measure each process vane thickness with digimatic calipers, average；Measure everywhere with acrylic glass flat ruler
The length of reason blade and width, average respectively；Each process fresh weight (W is weighed with electronic balanceFresh)；Oven drying method is utilized to exist
First 70 DEG C, rear 105 DEG C of (W that each process blade dried to constant weight in baking ovenDry), utilize mathematical method calculate variant blade (4n) with
Comparison blade (2n) water content: leaf water content=(WFresh–WDry)/WFresh× 100%.
Pore observation procedure
In fine day morning 8: 30～10: 00, take respectively and stop growing, there is obvious variation features blade (4n) and compare leaf
Each 10 of sheet (2n).It is coated with a thin layer with brush pen libation at an ancient wedding ceremony pyroxylin solution in blade back master pulse both sides, takes off with tweezers molten after 2～4min
Glue, removes blade back fine hair.Then smear one layer then at master pulse both sides, take off gel die, be placed in basis of microscopic observation and clap
According to.
Branches and tendrils rugosity and internode length observation procedure
Choose respectively and stopped growing or entered slowly growth, there are the branches and tendrils (4n) of obvious variation features and compare branches and tendrils
(2n) each 5, measure the 3rd～12 joint (5 × 10=50 joint) internode rugosity respectively, average；With acrylic glass flat ruler respectively
Measure the 3rd～12 joint (5 × 10=50 joint) panel lengths, average.
In fruit with exterior quality observation procedure
On JIUYUE 15th, 2015, from having obvious variation features branches and tendrils and compareing each 15 fringes of clip fruit ear branches and tendrils, uses electronics
Balance weighs Ear-Weight respectively, calculates 4n Yu 2n fruit ear and fruit grain average weight；With portable hand-held digital display saccharometer respectively
Measure said two devices soluble solid content, average.
Interpretation of result
The present embodiment, while employing goes the wall Low Osmotic Method stem apex to choosing to carry out microscopy, has observed chromosome number
(Fig. 1,2).Unit field of view being observed, comparison Vitis davidi chromosome number is 2n=2x=38, and the dye of Vitis davidi variation type
Colour solid number is mostly 2n → 4x=76, has a small amount of for types such as 2n=2x=38+4,2n=2x=38+6.
The pentyl xanthate ploidy mutagenic effect interpretation of result to Vitis davidi growth bud
As it can be seen from table 1 with after the pentyl xanthate corresponding bud of capsule shroud of variable concentrations, the germination rate of bud is with pentyne grass
The rising of amine concentration and reduce, and aberration rate is in rising trend in the range of concentration is 0.003%～0.005%, exceedes
0.005%, percentage blind (mortality rate of bud) raises, and aberration rate is the most suppressed and reduces.Accordingly, the pentyne of 0.005% concentration
Grass amine has best ploidy Mutagenic Effect to the Vitis davidi bud being in growth conditions, and its ploidy inductivity reaches 62.5%, and dyeing
Body quantity is become (2n → 4x=76) (see Fig. 1 and Fig. 2) by (2n=2x=38) mostly.
The pentyl xanthate of the different shroud concentration of table 1 grows bud ploidy Mutagenic Effect to Vitis davidi
Note: in table, small letter the English alphabet shows p 0.05
The pentyl xanthate morphology influence to Vitis davidi blade
Gather and there is 50, the blade (4n) of obvious variation features, 50, (2n) blade of comparison, from form, size, blade
The aspects such as thickness are observed and have been analyzed: observe in terms of leaf morphology, and comparison Vitis davidi blade (2n) is wealthy avette, by penta
Ploidy variation blade (4n) that the impact of alkynes grass amine produces is heart-shaped；It is more dark green that the leaf color ratio of variant blade compares, variant
Blade blade face is more crisp and more coarse；Produce the blade thicker (being shown in Table 2) than comparison of ploidy variation；Though comparison blade ratio produces
, there is the blade ratio of ploidy variation in the blade length of raw ploidy variation and width, but analysis in terms of the leaf water content height recorded
The water content of comparison is low, dry matter content of a relatively high (being shown in Table 2).
Table 2 Vitis davidi comparison (2n) compares with ploidy variant (4n) leaf morphology
Note: in table, small letter the English alphabet shows p 0.05
The pentyl xanthate impact on Stoma of Leaves form Yu size
The gel die taken off with the blade back compareing leaf from variant is placed under microscope and is observed: variant blade
The length and width of pore (4n) is all higher than compareing (2n), illustrates that the pore of variant blade is bigger than comparison.See Fig. 3 and Fig. 4, become
The stomatal frequency of allosome blade than comparison little (see Fig. 3,4).
The pentyl xanthate impact on Vitis davidi branches and tendrils rugosity Yu panel length
From table 3 it can be seen that compared with the Vitis davidi tetraploid panel length of variation is diplontic with comparison, internode substantially becomes
Short, it is the 54.40% of comparison；Branches and tendrils internode rugosity the most substantially attenuates, 50.10% only compareed.This life with autopolyploid
It is relevant that long speed is generally below diploid.
The impact on Vitis davidi branches and tendrils rugosity Yu panel length of table 3 pentyl xanthate
Note: in table, small letter the English alphabet shows p 0.05
The variation tetraploid Vitis davidi fruit maturation phase planted in biology electromechanics Vocationl Technical College liana garden, Hunan is with right
According to diplontic no significant difference, both of which is ripe 8/ last ten-days period～9/ early and middle ten days.After maturation, fruit grain color is all black-and-blue, face
Color depth is shallow there is no significant difference.From table 4, it can be seen that though 4n fruit ear and fruit grain are more slightly larger than 2n, the heaviest, but all it is not up to aobvious
Work level.The tetraploid soluble solid content that makes a variation reaches 19.39%, is substantially higher than the 11.15% of comparison.
The impact on fruit profile Yu soluble solid of table 4 pentyl xanthate
Note: in table, small letter the English alphabet shows p 0.05
Embodiment 2
The present embodiment also provides for the ploidy Mutagenic Effect test in the different shroud times of the Vitis davidi bud.Result of the test shows,
With the pentyl xanthate effect Vitis davidi bud 6 days of 0.005% concentration, its induced mutation rate reaches more than 60%, and ploidy Mutagenic Effect is still
Best；When shroud time lengthening, germination rate is decreased obviously.
The table 5 different shroud time grows bud ploidy Mutagenic Effect to Vitis davidi
Claims (5)
1. the ploidy method of mutagenesis of a pentyl xanthate shroud Vitis davidi bud, it is characterised in that comprise the following steps:
(1) pentyl xanthate is dissolved in dimethyl sulfoxide solvent, then is diluted with water to mass concentration 0.003%-of pentyl xanthate
0.007%, it is thus achieved that pentyl xanthate solution；
(2) described pentyl xanthate solution being made pentyl xanthate agar cream, set covers on the Vitis davidi bud being in bud
5-7 days, i.e. complete the ploidy mutation of Vitis davidi bud.
2. ploidy method of mutagenesis as claimed in claim 1, it is characterised in that dimethyl sulfoxide in described pentyl xanthate solution
Mass concentration is 0.04%-0.08%.
3. ploidy method of mutagenesis as claimed in claim 1, it is characterised in that by described under conditions of temperature is 13-18 DEG C
Pentyl xanthate agar cream set covers on Vitis davidi bud.
4. ploidy method of mutagenesis as claimed in claim 1, it is characterised in that the matter of pentyl xanthate in described pentyl xanthate solution
Amount concentration is 0.005%.
5. ploidy method of mutagenesis as claimed in claim 1, it is characterised in that the described shroud time is 6 days.
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| CN201610375056.4A CN105941141B (en) | 2016-05-31 | 2016-05-31 | The ploidy method of mutagenesis of pentyl xanthate shroud Vitis davidii Foex bud |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN116098058A (en) * | 2023-03-01 | 2023-05-12 | 南通大学 | Device and method for puncturing and vacuumizing to induce doubling of willow chromosomes |
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| CN103053377A (en) * | 2013-01-27 | 2013-04-24 | 管天球 | Vitis davidii foex culturing method |
| CN104719080A (en) * | 2015-04-16 | 2015-06-24 | 王道平 | Root-promoting and cutting seedling culture method for Fu'an brier grapes |
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| CN103053377A (en) * | 2013-01-27 | 2013-04-24 | 管天球 | Vitis davidii foex culturing method |
| CN104719080A (en) * | 2015-04-16 | 2015-06-24 | 王道平 | Root-promoting and cutting seedling culture method for Fu'an brier grapes |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN116098058A (en) * | 2023-03-01 | 2023-05-12 | 南通大学 | Device and method for puncturing and vacuumizing to induce doubling of willow chromosomes |
| CN116098058B (en) * | 2023-03-01 | 2023-09-08 | 南通大学 | Device and method for puncturing and vacuumizing to induce doubling of willow chromosomes |
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