CN104614970A - Optical scanning holographic image edge extracting method based on double-hole pupil - Google Patents
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Abstract
一种基于双孔光瞳的光学扫描全息图像边缘提取方法,属于光学扫描全息技术领域。技术方案包括:步骤1:将双孔光瞳设置为P2a(x,y)=δ(x-x1,y-y1),获得第一个菲涅尔波带板h1,进行第一次二维全息扫描,获取全息图s1。步骤2:将双孔光瞳设置为P2b(x,y)=δ(x-x2,y-y2),获得第二个菲涅尔波带板h2,进行第二次二维全息扫描,获取全息图s2,以记录更多的物体高频信息。步骤3:将两次二维全息扫描的全息图进行傅立叶变换,引入共轭梯度法进行逆问题求解。本发明结构简单,易操作,具有很强的实用性,能实现清晰的物像边缘提取,具有重要的应用价值。
The invention relates to an edge extraction method of an optical scanning holographic image based on a double hole pupil, which belongs to the technical field of optical scanning holography. The technical solution includes: Step 1: Set the double-hole pupil as P 2a (x,y)=δ(xx 1 ,yy 1 ), obtain the first Fresnel zone plate h 1 , and perform the first two-dimensional Scan the hologram to obtain the hologram s 1 . Step 2: Set the double aperture pupil as P 2b (x,y)=δ(xx 2 ,yy 2 ), obtain the second Fresnel zone plate h 2 , conduct the second two-dimensional holographic scan, and obtain The hologram s 2 is used to record more high-frequency information of the object. Step 3: Perform Fourier transform on the hologram of the two-dimensional holographic scanning, and introduce the conjugate gradient method to solve the inverse problem. The invention is simple in structure, easy to operate, has strong practicability, can realize clear object image edge extraction, and has important application value.
Description
技术领域technical field
本发明属于光学全息成像领域,涉及光学扫描全息技术和三维成像技术。The invention belongs to the field of optical holographic imaging, and relates to optical scanning holographic technology and three-dimensional imaging technology.
背景技术Background technique
光学扫描全息技术(Optical Scanning Holography,OSH)是数字全息技术中的一种,通过将光束分成不同的两个波前进行干涉形成菲涅尔波带板(FresnelZone Plate,FZP),进而实现对目标物体的高分辨率三维成像,它在生物医学成像、形变测量、粒子场测试、光学显微和光学遥感等领域都有广泛的应用前景。Optical Scanning Holography (OSH) is a kind of digital holography technology. By dividing the beam into two different wavefronts and interfering to form a Fresnel Zone Plate (FZP), and then realize the target High-resolution three-dimensional imaging of objects has broad application prospects in the fields of biomedical imaging, deformation measurement, particle field testing, optical microscopy, and optical remote sensing.
通过光学扫描全息技术获得的二维全息图,包含了样品完整的三维信息,因此如何由全息图提取出样品图像的边缘信息成为了光学扫描全息术的研究热点。图像边缘是图像的最基本的特征,图像的边缘检测也是图像处理的重要基础内容之一,具体对于光学扫描全息术来说,其难点在于如何由二维的全息图中提取出有效的重现图像边缘信息。The two-dimensional hologram obtained by optical scanning holography technology contains the complete three-dimensional information of the sample, so how to extract the edge information of the sample image from the hologram has become a research hotspot in optical scanning holography. The edge of an image is the most basic feature of an image, and image edge detection is also one of the important basic contents of image processing. Specifically for optical scanning holography, the difficulty lies in how to extract effective reproduction from a two-dimensional hologram. Image edge information.
文献‘Edge detection of three-dimensional objects by manipulating pupilfunctions in an optical scanning holography system’提出了一种基于光瞳设计的预处理边缘提取方法,通过将OSH系统中的两个光瞳分别设计为点脉冲函数(Diracdelta function)和拉普拉斯高斯函数(Laplacian of the Guassian),从而实现物像的边缘提取,但需要对实验系统做较大改动。The document 'Edge detection of three-dimensional objects by manipulating pupil functions in an optical scanning holography system' proposed a preprocessing edge extraction method based on pupil design, by designing the two pupils in the OSH system as point pulse functions (Diracdelta function) and Laplacian of the Guassian function (Laplacian of the Guassian), so as to realize the edge extraction of the object image, but it needs to make major changes to the experimental system.
文献‘Edge-preserving sectional image reconstruction in optical scanningholography’提出了一种利用总变差的非负约束法和梯度投影的方法,该方法能够强化切片物像,从而提取边缘信息,但是算法复杂度较高。The document 'Edge-preserving sectional image reconstruction in optical scanningholography' proposes a method using the non-negative constraint method of total variation and gradient projection. This method can strengthen the slice object image to extract edge information, but the algorithm complexity is high .
文献‘Edge extraction using a time-varying vortex beam in incoherent digitalholography’提出一种利用时变涡束来提取全息图边缘信息的方法,通过在OSH系统中引入螺旋相位板作为其中一个光瞳,从而获得时变涡束来提取物像边缘。该方法能获得较为清晰的边缘信息,但实验系统复杂度也有一定程度的上升。The document 'Edge extraction using a time-varying vortex beam in incoherent digitalholography' proposes a method of using time-varying vortex beams to extract edge information of holograms. By introducing a spiral phase plate in the OSH system as one of the pupils, the time-varying vortex beam in coherent digitalholography is obtained. Change the vortex beam to extract objects like edges. This method can obtain clearer edge information, but the complexity of the experimental system also increases to a certain extent.
发明内容Contents of the invention
本发明提供一种基于双孔光瞳的光学扫描全息图像边缘提取方法。在全息成像记录阶段,通过引入双孔光瞳,从而分别产生两个不同的菲涅尔波带板,接着利用这两个菲涅尔波带板对同一个被测样品进行扫描,获取两组全息图,以记录更多的物体高频信息;在全息成像重现阶段,通过傅立叶变换,将空域的全息图转换到频域,接下来再利用共轭梯度法和傅立叶逆变换,分别实现物像边缘及其本身的显像。该方法结构简单,便于操作,具有很强的实用性,能够实现清晰的物像边缘提取。The invention provides a method for extracting the edge of an optical scanning holographic image based on a double-hole pupil. In the holographic imaging recording stage, two different Fresnel zone plates are produced by introducing a double-hole pupil, and then the same sample to be tested is scanned by these two Fresnel zone plates to obtain two sets of Hologram to record more high-frequency information of the object; in the holographic imaging reproduction stage, the hologram in the spatial domain is converted to the frequency domain through Fourier transform, and then the conjugate gradient method and inverse Fourier transform are used to realize the object Representation of the image edge and itself. The method is simple in structure, easy to operate, has strong practicability, and can realize clear object image edge extraction.
本发明的技术方案为:一种基于双孔光瞳的光学扫描全息图像边缘提取方法,其系统结构如图1所示,包括以下步骤:The technical solution of the present invention is: an optical scanning holographic image edge extraction method based on a dual-hole pupil, the system structure of which is shown in Figure 1, including the following steps:
步骤1:偏振分束器(Beam Splitter,BS)将角频率为ω的光分成两部分,其中一部分通过第一光瞳P1(x,y)形成平面波P1(x,y)=1;另一部分经过声光调制器(Acoustic Optical Frequency Shifter,AOFS)产生Ω的频移后再通过第二光瞳P2(x,y)。这里,第二光瞳P2(x,y)由透射型液晶空间光调制器(Spacial LightModulator,SLM)实现。通过调节空间光调制器的电压分布,可以使得第二光瞳P2(x,y)工作在两个状态:(1)P2a(x,y)=δ(x-x1,y-y1),以及(2)P2b(x,y)=δ(x-x2,y-y2),以产生两个中心位置不同的球面波;其中,x1≠x2,y1≠y2,分别代表第二光瞳P2(x,y)在x,y轴上的空间偏移量;第一次全息图记录时,将分别通过第一光瞳P1(x,y)和第二光瞳P2a(x,y)的两束光经偏振分束器合在一起,在被测物体上产生干涉形成菲涅尔波带板h1,利用二维扫描镜控制h1的偏转,从而实现对三维样品的二维扫描。透镜3用于收集通过样品的透射光和散射光,并将其送入光电探测器。产生的外差电流输出经过混频、滤波、放大等处理,产生解调信息并储存于计算机中。储存的信息为h1编码图像,本质上为包含了样品三维信息的全息图。Step 1: A polarization beam splitter (Beam Splitter, BS) splits the light with an angular frequency ω into two parts, one of which passes through the first pupil P 1 (x, y) to form a plane wave P 1 (x, y)=1; The other part passes through the second pupil P 2 (x, y) after passing through the Acoustic Optical Frequency Shifter (AOFS) to generate a frequency shift of Ω. Here, the second pupil P 2 (x, y) is realized by a transmissive liquid crystal spatial light modulator (Spatial LightModulator, SLM). By adjusting the voltage distribution of the spatial light modulator, the second pupil P 2 (x,y) can work in two states: (1) P 2a (x,y)=δ(xx 1 ,yy 1 ), and (2)P 2b (x,y)=δ(xx 2 ,yy 2 ), to generate two spherical waves with different center positions; where, x 1 ≠x 2 , y 1 ≠y 2 , respectively represent the second light The spatial offset of pupil P 2 (x, y) on the x, y axis; when the first hologram is recorded, it will pass through the first pupil P 1 (x, y) and the second pupil P 2a ( The two beams of light x, y) are combined by a polarizing beam splitter to generate interference on the object to be measured to form a Fresnel zone plate h 1 , and the deflection of h 1 is controlled by a two-dimensional scanning mirror, thereby realizing the three-dimensional sample two-dimensional scanning. Lens 3 is used to collect the transmitted and scattered light passing through the sample and send it to the photodetector. The generated heterodyne current output is processed by frequency mixing, filtering, amplification, etc. to generate demodulation information and store it in the computer. The stored information is h 1 coded image, which is essentially a hologram containing three-dimensional information of the sample.
该光学扫描全息系统的空间脉冲响应,即菲涅尔波带板h1可以表示为The spatial impulse response of this optical scanning holographic system, that is, the Fresnel zone plate h can be expressed as
其中x,y,z代表空间坐标,k为光的波数。由(1)式可以看出,对于某一轴向位置z,菲涅尔波带板是一个关于x,y的二维对称函数,对称中心为(x1,y1)。Where x, y, z represent space coordinates, and k is the wave number of light. It can be seen from formula (1) that for a certain axial position z, the Fresnel zone plate is a two-dimensional symmetric function about x, y, and the center of symmetry is (x 1 , y 1 ).
假设复函数代表样品的幅度信息,则该样品经过光学系统扫描后得到的二维全息图可以表示为Assuming a complex function represents the amplitude information of the sample, then the two-dimensional hologram obtained after the sample is scanned by the optical system can be expressed as
其中*代表二维卷积。将待测样品看作一系列离散切片的集合,即可对轴向坐标z进行离散化处理,表示为z1,z2,...,zn,分别代表不同切片所在的轴向位置。那么(2)式表征的二维全息图可以表示为where * represents two-dimensional convolution. Considering the sample to be tested as a collection of a series of discrete slices, the axial coordinate z can be discretized, expressed as z 1 , z 2 ,...,z n , which represent the axial positions of different slices respectively. Then the two-dimensional hologram represented by (2) can be expressed as
假设样品仅包含一个切片,则(3)式可以简化为Assuming that the sample contains only one slice, formula (3) can be simplified as
我们将转换为一维矢量矩阵ψ。如果待测样品为一个N×N的矩阵,则ψ为长度为N2的一维矢量矩阵。同样,样品的二维全息图s1(x,y)以及菲涅尔波带板h1(x,y,z1)也可以分别转化为长度为N2的一维矢量矩阵S1和H1。we will Convert to a 1D vector matrix ψ. If the sample to be tested is an N×N matrix, then ψ is a one-dimensional vector matrix with length N2 . Similarly, the two-dimensional hologram s 1 (x, y) of the sample and the Fresnel zone plate h 1 (x, y, z 1 ) can also be transformed into one-dimensional vector matrices S 1 and H of length N 2 1 .
这样,(4)式可表示为In this way, (4) can be expressed as
S1=H1ψ+n1 (5)S 1 =H 1 ψ+n 1 (5)
其中n1代表系统的高斯白噪声,是长度为N2的一维矢量矩阵。where n 1 represents the white Gaussian noise of the system, which is a one-dimensional vector matrix of length N 2 .
步骤2:调节空间光调制器的电压,将P2(x,y)设置为P2b(x,y)=δ(x-x2,y-y2),其中,x1≠x2,y1≠y2,从而获得中心位置偏移的球面波。将新的球面波与另外一束平面波经偏振分束器合在一起,在被测物体上产生干涉形成第二个菲涅尔波带板(h2)。Step 2: Adjust the voltage of the spatial light modulator, set P 2 (x,y) as P 2b (x,y)=δ(xx 2 ,yy 2 ), where x 1 ≠x 2 , y 1 ≠y 2 , so as to obtain the spherical wave whose center position is shifted. Combine the new spherical wave and another beam of plane wave through the polarization beam splitter, and generate interference on the measured object to form the second Fresnel zone plate (h 2 ).
该光学扫描全息系统的空间脉冲响应,即菲涅尔波带板(h2)可以表示为The spatial impulse response of the optical scanning holographic system, that is, the Fresnel zone plate (h 2 ), can be expressed as
将(6)式与(1)式对比可以看出,通过调节第二光瞳P2(x,y),可以利用同一套光学系统,设计实现不同的菲涅尔波带板,以获取更多的物体高频信息。Comparing Equation (6) with Equation (1), it can be seen that by adjusting the second pupil P 2 (x,y), the same optical system can be used to design different Fresnel zone plates to obtain more High-frequency information of many objects.
通过对同一待测样品进行扫描,可获得第二组样品全息图。同样该过程可以表征为By scanning the same sample to be tested, a second group of sample holograms can be obtained. Likewise, this process can be characterized as
矩阵方程为The matrix equation is
(8) (8)
S2=H2ψ+n2 S 2 =H 2 ψ+n 2
将两次二维全息扫描的矩阵方程整合起来,可以表示为Integrating the matrix equations of the two 2D holographic scans, it can be expressed as
步骤3:利用两次二维扫描的全息图进行全息成像的重现。在全息成像重现阶段,首先通过傅立叶变换,将空域的全息图转换到频域,接下来再利用共轭梯度法和傅立叶逆变换,分别实现物像边缘及其本身的显像。全息成像重现,即要在已知S的情况下,求解目标矢量ψ。该问题的求解可转化为如下的最小化问题,Step 3: Reconstruct the holographic imaging by using the hologram scanned twice in two dimensions. In the stage of holographic imaging reproduction, the hologram in the spatial domain is first converted to the frequency domain by Fourier transform, and then the conjugate gradient method and inverse Fourier transform are used to realize the imaging of the edge of the object image and itself respectively. The reconstruction of holographic imaging is to solve the target vector ψ under the condition of known S. The solution of this problem can be transformed into the following minimization problem,
其中||.||代表二阶范数,λ>0为罚系数,C是拉普拉斯高斯算子,其本质为高通滤波器,能够用于物像的边缘提取。该最小化问题的解可表示为Where ||.|| represents the second-order norm, λ>0 is the penalty coefficient, and C is the Laplacian Gaussian operator, which is essentially a high-pass filter and can be used for edge extraction of objects. The solution to this minimization problem can be expressed as
(H+H+λC+C)f(ψ)=H+G (11)(H + H + λC + C)f(ψ) = H + G (11)
其中H+为矩阵H的共轭转置。通过引入共轭梯度算法,即可实现边缘提取以及物像重现。where H + is the conjugate transpose of matrix H. By introducing the conjugate gradient algorithm, edge extraction and object image reproduction can be realized.
本发明的有益效果是:通过利用双孔光瞳形成不同菲涅尔波带板对待测样品分别进行扫描,获得两个二维全息图,从而记录下更多的物体高频信息,接着通过傅立叶变换,将空域的全息图转换到频域,再利用共轭梯度法和傅立叶逆变换,分别实现物像边缘及其本身的显像。该方法结构简单,易于操作,可以实现清晰的物像边缘提取,具有重要的应用价值。The beneficial effects of the present invention are: by using the double-hole pupils to form different Fresnel zone plates to scan the sample to be tested respectively, two two-dimensional holograms are obtained, thereby recording more high-frequency information of the object, and then through Fourier The hologram in the spatial domain is transformed into the frequency domain, and then the conjugate gradient method and the inverse Fourier transform are used to realize the imaging of the edge of the object image and itself. The method is simple in structure, easy to operate, and can realize clear object image edge extraction, which has important application value.
附图说明Description of drawings
图1为本发明基本结构图(BS:偏振分束器,AOFS:声光调制器,lens:透镜,2D scan:二维扫描,detector:光电探测器);Fig. 1 is a basic structural diagram of the present invention (BS: polarization beam splitter, AOFS: acousto-optic modulator, lens: lens, 2D scan: two-dimensional scanning, detector: photodetector);
图2为本发明具体实施方式中待测样品示意图;Fig. 2 is a schematic diagram of a sample to be tested in a specific embodiment of the present invention;
图3为本发明具体实施方式中第二光瞳P2a(x,y)和P2b(x,y)对应的菲涅尔波带图;Fig. 3 is the Fresnel zone figure corresponding to the second pupil P 2a (x, y) and P 2b (x, y) in the specific embodiment of the present invention;
图4为本发明具体实施方式中第二光瞳P2a(x,y)和P2b(x,y)分别扫描获得的正弦全息图;4 is a sinusoidal hologram obtained by scanning the second pupil P 2a (x, y) and P 2b (x, y) respectively in a specific embodiment of the present invention;
图5为采用传统方法获取的样品图像重现效果;Figure 5 is the sample image reproduction effect obtained by traditional methods;
图6为本发明具体实施方式中获取的样品图像重现效果:包括物像本身及其边缘信息。Fig. 6 is a sample image reproduction effect obtained in a specific embodiment of the present invention: including the object image itself and its edge information.
具体实施方式Detailed ways
下面结合附图对本发明进行进一步说明。The present invention will be further described below in conjunction with the accompanying drawings.
一种基于双孔光瞳的光学扫描全息图像边缘提取方法,包括以下步骤:A method for extracting the edge of an optical scanning holographic image based on a double-hole pupil, comprising the following steps:
步骤1:将双孔光瞳设置为P2a(x,y)=δ(x-x1,y-y1),获得第一个菲涅尔波带板h1,进行第一次二维全息扫描,获取全息图。Step 1: Set the double aperture pupil as P 2a (x,y)=δ(xx 1 ,yy 1 ), obtain the first Fresnel zone plate h 1 , perform the first two-dimensional holographic scan, and obtain Hologram.
步骤2:将双孔光瞳设置为P2b(x,y)=δ(x-x2,y-y2),获得第二个菲涅尔波带板h2,进行第二次二维全息扫描,获取全息图。Step 2: Set the double aperture pupil as P 2b (x,y)=δ(xx 2 ,yy 2 ), obtain the second Fresnel zone plate h 2 , conduct the second two-dimensional holographic scan, and obtain Hologram.
步骤3:将两次二维全息扫描的全息图进行傅立叶变换,引入共轭梯度法进行逆问题求解。Step 3: Perform Fourier transform on the hologram of the two-dimensional holographic scanning, and introduce the conjugate gradient method to solve the inverse problem.
本实施例具体结构如图1所示,使用的单波长光源中心波长为632nm。本实施例中采用的待测样品如图2所示,样品所在轴向位置为z=34mm,切片尺寸为1mm×1mm,矩阵尺寸为512×512。The specific structure of this embodiment is shown in FIG. 1 , and the center wavelength of the single-wavelength light source used is 632 nm. The sample to be tested used in this embodiment is shown in FIG. 2 , the axial position of the sample is z=34mm, the slice size is 1mm×1mm, and the matrix size is 512×512.
首先将第二光瞳设为P2a(x,y)=δ(x-x1,y-y1),其中x1=0mm,y1=0mm,进行第一次二维全息扫描,获取全息图s1。First, set the second pupil as P 2a (x,y)=δ(xx 1 ,yy 1 ), where x 1 =0mm, y 1 =0mm, perform the first two-dimensional holographic scanning, and obtain the hologram s 1 .
接着将第二光瞳设为P2(x,y)=δ(x-x2,y-y2),其中x2=0.8mm,y2=0mm,进行第二次二维全息扫描,获取全息图s2。Next, set the second pupil as P 2 (x,y)=δ(xx 2 ,yy 2 ), where x 2 =0.8mm, y 2 =0mm, and conduct the second two-dimensional holographic scanning to obtain the hologram s 2 .
两次扫描中,不同光瞳获得的菲涅尔波带图如图3所示。由图3(a)和(b)可见,通过改变光瞳的位置,可以记录物体更多的高频信息,从而实现物像边缘信息的强化和提取。两次扫描获得的正弦全息图如图4所示。由图4可见,我们无法从全息图中获取任何待测样品的信息。Fresnel zone diagrams obtained with different pupils in the two scans are shown in Figure 3. It can be seen from Figure 3(a) and (b) that by changing the position of the pupil, more high-frequency information of the object can be recorded, thereby realizing the enhancement and extraction of the edge information of the object image. The sinusoidal hologram obtained by two scans is shown in Fig. 4. It can be seen from Figure 4 that we cannot obtain any information about the sample to be tested from the hologram.
接下来,我们比较了两种切片成像方法:(1)传统样品图像重现方法,即用切片处的菲涅尔波带片共轭函数与样品全息图进行卷积;(2)基于双孔光瞳的边缘提取方法。图5(a)和(b)分别显示了利用全息图s1和全息图s2进行传统图像重现的结果,可以看出,传统图像重现的效果很不理想,图像边缘很模糊。而采用本发明的方法,则可以完全区分出物像及其边缘信息,其结果如图6(a)和(b)所示。这意味着,本发明方法成功将物像边缘信息提取了出来。Next, we compared two slice imaging methods: (1) the traditional sample image reconstruction method, which convolutes the sample hologram with the Fresnel zone plate conjugate function at the slice; Pupil edge extraction method. Figure 5(a) and (b) show the results of traditional image reconstruction using hologram s 1 and hologram s 2 , respectively. It can be seen that the effect of traditional image reproduction is not ideal, and the edges of the image are blurred. With the method of the present invention, the object image and its edge information can be completely distinguished, and the results are shown in Fig. 6 (a) and (b). This means that the method of the present invention successfully extracts the edge information of the object image.
实施例证明,通过引入双孔光瞳,能利用不同的菲涅尔波带板对待测样品进行扫描,获取两组全息图,从而记录更多的物体高频信息,接下来利用傅立叶变换将全息图转换到频域,引入共轭梯度算法进行求解。该方法结构简单,易于操作,具有很强的实用性,能够实现清晰的图片边缘提取,具有重要的应用价值。The embodiment proves that by introducing a double-hole pupil, different Fresnel zone plates can be used to scan the sample to be tested to obtain two sets of holograms, thereby recording more high-frequency information of the object, and then using Fourier transform to convert the hologram The graph is converted to the frequency domain, and the conjugate gradient algorithm is introduced to solve it. The method is simple in structure, easy to operate, has strong practicability, can realize clear image edge extraction, and has important application value.
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