CN104614393B - A kind of assay method of baccy fiber cellulose content - Google Patents
A kind of assay method of baccy fiber cellulose content Download PDFInfo
- Publication number
- CN104614393B CN104614393B CN201510073837.3A CN201510073837A CN104614393B CN 104614393 B CN104614393 B CN 104614393B CN 201510073837 A CN201510073837 A CN 201510073837A CN 104614393 B CN104614393 B CN 104614393B
- Authority
- CN
- China
- Prior art keywords
- cellulose
- sample
- peak area
- measured
- internal standard
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Abstract
The invention discloses a kind of assay method of baccy fiber cellulose content, it is characterized in that：Weigh the cellulose standard sample powder of different quality first, it is placed in the sample cell with silica gel tube as internal standard substance, carbon 13 cross polarization magic-angle-spinning nuclear magnetic resonance spectrometer is tested, obtain the wave spectrogram of each cellulose standard sample, using PeakFit software processes, obtain the quantitative peak area of same wave spectrogram Plays sample and the ratio of the quantitative peak area of internal standard substance, the absolute mass of cellulose is as abscissa with standard sample, mapped for vertical coordinate with ratio, obtain standard working curve；Then repeat the above steps, obtain the ratio of the quantitative peak area of the quantitative peak area of testing sample and internal standard substance in tobacco sample wave spectrogram to be measured, again by standard working curve, obtain the absolute mass of cellulose in tobacco sample to be measured, and then obtain tobacco sample content of cellulose to be measured.The present invention is without pre-treatment, quantitative accurate, is suitable for mass and measures baccy fiber cellulose content.
Description
Technical field
The present invention relates to a kind of assay method of baccy fiber cellulose content, belong to Tobacco Analysis chemical research field.
Background technology
Baccy fiber element is a kind of important biological tobacco polymer, mainly plays machine in the growth course of tobacco plant
Tool supporting function.To the combustibility of Nicotiana tabacum L. and Medicated cigarette is jealous has a direct impact for cellulose, the high Nicotiana tabacum L. burning of content of cellulose
Property good, holding firepower strengthens, but gives the abnormal smells from the patient of the zest of flue gas spine and " burning paper as sacrificial offerings " after burning.So, baccy fiber element
Content is a valuable parameter characterizing cigarette products, and in accurate quantitative analysis tobacco sample, the content of cellulose is joined for Medicated cigarette
Square design sense is great.
At present, tobacco business measure Nicotiana tabacum L. in content of cellulose substantially adopt chemical method, that is, first using neutral detergent,
Acid detergent and 72% concentrated sulphuric acid remove the solable matter in tobacco sample respectively, then sample drying, after ashing,
Obtain neutral detergent fiber, acid detergent fiber and pickling content of lignin using gravimetric method, respectively obtain sample by calculating
The content of middle cellulose, hemicellulose and lignin.Therefore, chemical gauging baccy fiber element process is loaded down with trivial details, takes longer,
And a large amount of chemical reagent need to be used, easily cause environmental pollution it is difficult to meet tobacco business rapid batchization to measure baccy fiber
The current demand of cellulose content.NMR (Nuclear Magnetic Resonance) spectrum technology is a kind of very outstanding, quick detection means, is widely used in thing
The mensure of matter structure；Because the peak area of NMR (Nuclear Magnetic Resonance) spectrum in figure formant or peak height are directly just become with the content of tested component
Than, so, this technology also apply be applicable to the mensure of content of material in testing sample.In recent years, Hall and Wooten (Hall R
A,Wooten JB.Quantitative Analysis of Cellulose in Tobacco by 13C CPMAS
NMR.J.Agric.Food Chem.1998,46,1423-1427.) adopt carbon 13 cross polarization magic-angle-spinning nuclear magnetic resonance first
Spectrometer is measured to content of cellulose in tobacco sample, and compared with traditional chemical method, this analysis method is greatly
Improve the detection speed of sample.But because Nicotiana tabacum L. is the sufficiently complex chemical system of a composition, in order to eliminate other things
The interference of confrontation cellulose, before measurement, Hall and Wooten tobacco sample to be measured is placed in hot water is washed first,
Obtain water-insoluble and the hydrotrope, then this two classes component is dried, and test water-insoluble and the hydrotrope after drying respectively
NMR (Nuclear Magnetic Resonance) spectrum figure, in data processing, using the NMR (Nuclear Magnetic Resonance) spectrum figure of the hydrotrope as background from the core of water-insoluble
Magnetic Resonance Spectrum in figure is deducted, and yet suffers from complex and time-consuming pretreatment process in this way；And
In the research process of Hall and Wooten, do not find the suitable internal standard substance for solid sample detection, to eliminate instrument itself
The impact that the fluctuation of signal causes to testing result, thus the correlation coefficient that result in its standard working curve is not ideal enough；Separately
Outward, during different sample tests, in order to save the pre-treatment time, Hall and Wooten is all by the hydrotrope of same sample
NMR (Nuclear Magnetic Resonance) spectrum figure is as the testing background of all samples, thus also systematic error can be brought to measurement result.Therefore, its tool
The application of body receives great limitation.
Content of the invention
For overcoming the defect of prior art, it is an object of the invention to provide a kind of process is simple, without using any molten
Agent carries out pre-treatment, and the assay method of the good baccy fiber cellulose content of quantitative effect, with meet tobacco business quick, convenient,
Mass measures the current demand of baccy fiber cellulose content.
The present invention solves technical problem and adopts the following technical scheme that：
The assay method of baccy fiber cellulose content of the present invention, is characterized in, the method comprising the steps of：
(1) draw standard working curve
Weigh a series of cellulose standard sample powder of different qualities, be added separately to the sample with silica gel tube as internal standard substance
In QC, then described sample cell is placed in carbon 13 cross polarization magic-angle-spinning nuclear magnetic resonance spectrometer and is tested, obtain
The wave spectrogram of each cellulose standard sample；
Using the cellulose being located at 105.5ppm in the wave spectrogram to gained each cellulose standard sample for the PeakFit software
Peak and the internal standard substance peak being located at 0ppm are processed, and obtain the quantitative peak area of cellulose and internal standard in same wave spectrogram respectively
The quantitative peak area of thing, then calculates the quantitative peak area of cellulose and internal standard in the wave spectrogram obtain each cellulose standard sample
The ratio of the quantitative peak area of thing；
With in each cellulose standard sample, the absolute mass of cellulose is as abscissa, with the wave spectrum of each cellulose standard sample
The quantitative peak area of in figure cellulose is vertical coordinate mapping with the ratio of the quantitative peak area of internal standard substance, obtains standard work bent
Line；
(2) test of tobacco sample to be measured
Weigh the tobacco sample powder to be measured of certain mass, be added in the sample cell with silica gel tube as internal standard substance, then
Described sample cell is placed in carbon 13 cross polarization magic-angle-spinning nuclear magnetic resonance spectrometer and is tested, obtain tobacco sample to be measured
Wave spectrogram；
Using in the wave spectrogram to gained tobacco sample to be measured for the PeakFit software be located at 105.5ppm place cellulose peak with
Internal standard substance peak at 0ppm is processed, and obtains in the wave spectrogram of tobacco sample to be measured the quantitative peak area of cellulose and interior
The quantitative peak area of mark thing, then calculates the quantitative peak area of cellulose and internal standard substance in the wave spectrogram obtain tobacco sample to be measured
Quantitative peak area ratio；
By the ratio of the quantitative peak area of quantitative peak area and the internal standard substance of cellulose in the wave spectrogram of tobacco sample to be measured,
Obtain the absolute mass of cellulose in described tobacco sample to be measured according to the standard working curve that step (1) is obtained, further according to
The quality of the absolute mass of cellulose and testing sample in tobacco sample to be measured, in acquisition tobacco sample to be measured, cellulose contains
Amount.
The above-mentioned sample cell with silica gel tube as internal standard substance, is the silica gel taking an external diameter to match with sample bore
Pipe, inserts it in sample cell, is then added thereto to sample again.
Compared with the prior art, beneficial effects of the present invention are embodied in：
1st, test process of the present invention is simple, fast and easy, environmental protection, and measurement result is accurately and reliably, is applicable to mass
Measure baccy fiber cellulose content.
2nd, adopt PeakFit software to the sample peak in wave spectrogram and internal standard peak, carry out baseline correction, second dervative, Fu
In leaf deconvolute and curve fit process, can effectively eliminate the interference of impurity peaks, it is to avoid criticize using NMR (Nuclear Magnetic Resonance) spectrum technology
Washing before quantitative determination baccy fiber cellulose content and drying course and the human and material resources that cause and waste of time.
3rd, adopt and at 0ppm, only produce nmr spectroscopic signal peak without cellulose peak domain is produced with the silicon of interference
Sebific duct, as internal standard substance, can eliminate shadow measurement result being brought because of the fluctuation of itself detection signal of instrument well
Ring；On the other hand, the silica gel tube external diameter by being used just is mated with sample bore, so also can effectively eliminate sample
Because of the high-speed rotation of sample cell during test, the internal standard signal fluctuation causing internal standard substance to shift and causing.
Brief description
Fig. 1 is the NMR (Nuclear Magnetic Resonance) spectrum figure of 5 cellulose standard sample in embodiment 1.
Fiber at 105.5ppm for the wave spectrogram of the cellulose standard sample that Fig. 2 is 35.5mg for quality in embodiment 1
Original spectral line, matching spectral line and each sub- peak that plain peak obtains through PeakFit v4.12 software processes, wherein peak 3 are cellulose
Quantitative peak, peak 1 and peak 2 are impurity peaks.
Internal standard substance peak at 0ppm for the wave spectrogram of the cellulose standard sample that Fig. 3 is 35.5mg for quality in embodiment 1
Original spectral line, matching spectral line and each sub- peak obtaining through PeakFit v4.12 software processes, wherein peak 2 are determining of internal standard substance
Amount peak, peak 1 and peak 3 are impurity peaks.
Fig. 4 is the standard working curve figure in embodiment 1.
Fig. 5 is the cellulose peak warp at 105.5ppm for the wave spectrogram of tobacco sample (cloud and mist 87X2F) to be measured in embodiment 1
Original spectral line, matching spectral line and each sub- peak that PeakFit v4.12 software processes obtain, wherein peak 3 are the quantitation of cellulose
Peak, peak 1 and peak 2 are impurity peaks.
Fig. 6 is the internal standard substance peak warp at 0ppm for the wave spectrogram of tobacco sample (cloud and mist 87X2F) to be measured in embodiment 1
Original spectral line, matching spectral line and each sub- peak that PeakFit v4.12 software processes obtain, wherein peak 2 are the quantitation of internal standard substance
Peak, peak 1 and peak 3 are impurity peaks.
Specific embodiment
Below by way of specific embodiment, and the invention will be further described to combine accompanying drawing.
In the embodiment of the present invention, the preparation condition of all fibres element standard sample powder and tobacco material powder to be measured is：Will
Cellulose standard sample and tobacco material to be measured, at 40 DEG C, after drying through 24 hours, are ground into 40 mesh, and airtight preservation is standby.
The instrument condition of nuclear magnetic resonance, NMR is：The carbon 13 cross polarization magic-angle-spinning nuclear magnetic resonance wave spectrogram of all samples,
C13 frequency is to obtain on Bruker AVANCE III 400 nuclear magnetic resonance chemical analyser of 100.63MHz；Signal to noise ratio is passed through to intersect
Polarize and to improve, cross polarization time is 2ms；Formant at silica gel tube 0ppm, as chemical shift and intensity reference, is tested
Using the Bruker probe of 7mm, sample is positioned over external diameter and is 7mm and includes in the nuclear magnetic resonance sample pipe of silica gel tube and surveyed
Examination；Magic angle is 54.7 °, and rotor speed is 4KHz, and sampling number is 2048, and the sampling interval is 2s.
Embodiment 1
First, accurately weighing 35.5mg, 60.6mg, 90.2mg, 119.7mg and 148.0mg content of cellulose respectively is
90.47% cellulose standard sample powder, is sequentially placed in the sample cell with silica gel tube as internal standard substance, in carbon 13 cross polarization
Tested on magic-angle-spinning nuclear magnetic resonance spectrometer, obtained the wave spectrogram of each sample, as shown in Figure 1.
Then, to the wave spectrogram of 5 cellulose standard sample in Fig. 1 at the plain peak of the dimension at 105.5ppm with 0ppm
Internal standard substance peak, baseline correction is carried out using PeakFit v4.12 software, second dervative, Fourier deconvolute and curve matching at
Reason, obtains the quantitative peak area of the quantitative peak area of cellulose and internal standard substance in same spectrogram.
Fig. 2 gives in 5 cellulose standard sample, and quality is that the cellulose standard sample wave spectrogram of 35.5mg exists
Primitive curve, matched curve and each sub- peak that cellulose peak at 105.5ppm obtains through PeakFit v4.12 software processes,
Wherein peak 3 is the quantitative peak of cellulose, and peak 1 and peak 2 are impurity peaks.
Fig. 3 gives in 5 cellulose standard sample, and quality is the cellulose standard sample wave spectrogram of 35.5mg
Primitive curve, matched curve and each sub- peak that internal standard substance peak at 0ppm obtains through PeakFit v4.12 software processes, its
Middle peak 2 is the quantitative peak of internal standard substance, and peak 1 and peak 3 are impurity peaks.
Then, with absolute mass m of cellulose in 5 standard samplei(i=1,2,3 ... ..) are abscissa, with same spectrum
The ratio I of the quantitative peak area of the quantitative peak area of in figure cellulose and internal standard substancei(i=1,2,3 ... ..) map for vertical coordinate, obtain
To standard working curve, as shown in Figure 4.
Finally, accurately weigh 198.2mg tobacco sample to be measured powder (cloud and mist 87X2F), repeat at above-mentioned test data
Reason, testing sample wave spectrogram is soft through PeakFit v4.12 at the cellulose peak at 105.5ppm and the internal standard substance peak at 0ppm
Primitive curve, matched curve and each sub- peak that part process obtains, respectively referring to Fig. 5 and Fig. 6；According in peak in Fig. 53 and Fig. 6
The peak area at peak 2, obtaining the quantitative peak area of cellulose and the ratio Ix of the quantitative peak area of internal standard substance is 0.52；Then in Fig. 4
Standard working curve in, with Ix replace Ii, with mxReplace mi, it is hereby achieved that in tobacco sample to be measured cellulose absolute
Quality mxFor 17.00mg, and the quality according to tobacco sample to be measured, you can the content obtaining cellulose in tobacco sample to be measured is
8.58%.
Embodiment 2
Weigh 0.2g (standard claims to 0.0002g) tobacco sample powder to be measured (cloud and mist 87X2F), parallel 5 parts of (sample number into spectrum 1-
5) the test data, repeating embodiment 1 is processed, and the meansigma methodss, standard deviation and the relative standard that obtain 5 retests are inclined
Difference, the results are shown in Table shown in 1.
The meansigma methodss of 15 retests of table, standard deviation and relative standard deviation
| Sample number into spectrum | Nuclear magnetic resonance measuring value (%) |
| 1 | 8.58 |
| 2 | 8.72 |
| 3 | 8.26 |
| 4 | 8.93 |
| 5 | 8.59 |
| Meansigma methodss | 8.62 |
| Standard deviation | 0.24 |
| Relative standard deviation (%) | 2.83 |
Embodiment 3
Take 8 to grow tobacco sample (sample number into spectrum 1# 8#), each sample be divided into two parts, a according to tobacco business standard
《Nicotiana tabacum L. and tobacco product cellulose, hemicellulose, the mensure Detergent method of lignin》Method is tested, and another is according to reality
Apply example 1 methods described step to be tested, the results are shown in Table shown in 2.
Table 2 this method is compared with the measurement result of standard method to the measurement result of cellulose in tobacco sample
Therefore, the present invention provide baccy fiber cellulose content assay method, not only test process simple, quick and convenient,
Time saving and energy saving, and measurement result is accurately and reliably, be tobacco business mass measure baccy fiber cellulose content and provide one kind
Effectively technical method and means.
Claims (1)
1. a kind of assay method of baccy fiber cellulose content, is characterized in that, the method comprising the steps of：
(1) draw standard working curve
Weigh a series of cellulose standard sample powder of different qualities, be added separately to the sample cell with silica gel tube as internal standard substance
In, then described sample cell is placed in carbon 13 cross polarization magic-angle-spinning nuclear magnetic resonance spectrometer and is tested, obtain each fibre
The wave spectrogram of the plain standard sample of dimension；The external diameter of described silica gel tube is matched with the internal diameter of described sample cell；
Using in the wave spectrogram to gained each cellulose standard sample for the PeakFit software be located at 105.5ppm place cellulose peak with
Internal standard substance peak at 0ppm is processed, and obtains the quantitative peak area of cellulose and internal standard substance in same wave spectrogram respectively
Quantitative peak area, then calculates the quantitative peak area of cellulose and internal standard substance in the wave spectrogram obtain each cellulose standard sample
The ratio of quantitative peak area；
With in each cellulose standard sample, the absolute mass of cellulose is as abscissa, with the wave spectrogram of each cellulose standard sample
The quantitative peak area of cellulose is vertical coordinate mapping with the ratio of the quantitative peak area of internal standard substance, obtains standard working curve；
(2) test of tobacco sample to be measured
Weigh the tobacco sample powder to be measured of certain mass, be added in the sample cell with silica gel tube as internal standard substance, then by institute
State sample cell and be placed in carbon 13 cross polarization magic-angle-spinning nuclear magnetic resonance spectrometer and tested, obtain the ripple of tobacco sample to be measured
Spectrogram；
Using in the wave spectrogram to gained tobacco sample to be measured for the PeakFit software be located at 105.5ppm at cellulose peak and be located at
Internal standard substance peak at 0ppm is processed, and obtains the quantitative peak area of cellulose and internal standard substance in the wave spectrogram of tobacco sample to be measured
Quantitative peak area, then calculate obtain tobacco sample to be measured wave spectrogram in the quantitative peak area of cellulose and determining of internal standard substance
The ratio of amount peak area；
By the ratio of the quantitative peak area of quantitative peak area and the internal standard substance of cellulose in the wave spectrogram of tobacco sample to be measured, according to
The standard working curve that step (1) is obtained obtains the absolute mass of cellulose in described tobacco sample to be measured, further according to be measured
In tobacco sample, the absolute mass of cellulose and the quality of testing sample, obtain the content of cellulose in tobacco sample to be measured.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510073837.3A CN104614393B (en) | 2015-02-11 | 2015-02-11 | A kind of assay method of baccy fiber cellulose content |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510073837.3A CN104614393B (en) | 2015-02-11 | 2015-02-11 | A kind of assay method of baccy fiber cellulose content |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN104614393A CN104614393A (en) | 2015-05-13 |
| CN104614393B true CN104614393B (en) | 2017-03-01 |
Family
ID=53148938
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201510073837.3A Active CN104614393B (en) | 2015-02-11 | 2015-02-11 | A kind of assay method of baccy fiber cellulose content |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN104614393B (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106908469A (en) * | 2017-03-21 | 2017-06-30 | 苏州大学 | The quantitative analysis method of constituent content in a kind of polytetrafluoroethylblended blended thing |
Families Citing this family (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105928763A (en) * | 2016-07-06 | 2016-09-07 | 中国科学技术大学 | Separation and extraction method and analysis method of flue-cured tobacco leaf starch |
| CN106468670A (en) * | 2016-09-29 | 2017-03-01 | 河北中烟工业有限责任公司 | A kind of tobacco moisture percentage method of testing based on time domain nuclear magnetic resonance, NMR inverting peak area |
| CN106501296B (en) * | 2016-10-18 | 2018-08-24 | 广州天赐高新材料股份有限公司 | The detection method of silicon hydrogen content in organosilicon polymer |
| CN106841272B (en) * | 2017-03-21 | 2019-02-05 | 苏州大学 | A kind of quantitative analysis method suitable for compound molecule group or blend component ratio |
| CN107505348A (en) * | 2017-08-09 | 2017-12-22 | 中国农业科学院农业质量标准与检测技术研究所 | Application of the metronidazole as internal standard compound in hydrogen nuclear magnetic resonance technology |
| CN113136689A (en) * | 2020-01-17 | 2021-07-20 | 青岛海尔洗衣机有限公司 | Paper foreign matter detection method for washing machine |
| CN111855730A (en) * | 2020-08-12 | 2020-10-30 | 黄河三角洲京博化工研究院有限公司 | Method for testing water content in butyl rubber glue solution |
| CN113267524B (en) * | 2021-05-14 | 2022-08-05 | 广东省中山市质量计量监督检测所 | Detection method of starch-based plastic component |
Family Cites Families (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH0641926B2 (en) * | 1985-12-26 | 1994-06-01 | ダイセル化学工業株式会社 | Method for analyzing hydroxyalkyl cellulose |
| JP2005337958A (en) * | 2004-05-28 | 2005-12-08 | Kao Corp | Analyzing method of water soluble compound having sugar skeleton |
| RU2558433C1 (en) * | 2014-02-17 | 2015-08-10 | Федеральное государственное бюджетное учреждение науки Южный научный центр Российской академии наук (ЮНЦ РАН) | Method of determining quantitative content of deuterium in water and aqueous solutions |
| CN103837560B (en) * | 2014-03-07 | 2016-04-06 | 中国烟草总公司郑州烟草研究院 | Low-field nuclear magnetic resonance measures the method for tobacco moisture percentage |
-
2015
- 2015-02-11 CN CN201510073837.3A patent/CN104614393B/en active Active
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106908469A (en) * | 2017-03-21 | 2017-06-30 | 苏州大学 | The quantitative analysis method of constituent content in a kind of polytetrafluoroethylblended blended thing |
| CN106908469B (en) * | 2017-03-21 | 2018-12-21 | 苏州大学 | The quantitative analysis method of constituent content in a kind of polytetrafluoroethylblended blended object |
Also Published As
| Publication number | Publication date |
|---|---|
| CN104614393A (en) | 2015-05-13 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN104614393B (en) | A kind of assay method of baccy fiber cellulose content | |
| CN107084995B (en) | A kind of quantitative evaluation method of density of tobacco rod distributing homogeneity | |
| CN106468670A (en) | A kind of tobacco moisture percentage method of testing based on time domain nuclear magnetic resonance, NMR inverting peak area | |
| CN105510469B (en) | The detection method of furfural content in a kind of transformer insulating paper | |
| CN109613042A (en) | A kind of pine class sawn timber Oleoresin Contents test method based on time domain nuclear magnetic resonance | |
| CN104931454A (en) | Method for rapidly determining Lycra fiber content of textiles using near infrared spectroscopic analysis | |
| CN103018148A (en) | Method for measuring porosity of coal core | |
| CN108627468A (en) | A kind of prediction technique of feeding Boehmeria nivea leaves crude fiber content | |
| CN108181397A (en) | Hangzhou chili capsaicine concentration extraction measuring method | |
| CN104596979A (en) | Method for measuring cellulose of reconstituted tobacco by virtue of near infrared reflectance spectroscopy technique | |
| CN1987431A (en) | Raman spectrum quick quantitative analytic method for bamboo-wood material main chemical components | |
| CN110887860A (en) | Method for detecting water content of sand based on low-field nuclear magnetic resonance | |
| CN102288569A (en) | Method for rapidly analyzing fiber biomass | |
| CN102928356A (en) | Method for measuring essence solvent content rapidly | |
| CN103424463A (en) | Protein fingerprint atlas-spectrum model of acidovorax avenae subsp. citrulli and applications thereof | |
| CN103675112B (en) | A kind of method measuring glyceroltriacetate in cigarette filter rods applied amount homogeneity | |
| CN111337529A (en) | Preparation and test method of polysaccharide sample with reduced viscosity and shifted water peak | |
| CN104122345B (en) | The assay method of 1,1,1-trimethylolpropane in cigarette paper using | |
| CN111060546A (en) | Cigarette moisture retention performance evaluation method based on low-field nuclear magnetic resonance technology | |
| CN103323406B (en) | A kind of fresh tea leaf fluorine content non-destructive rapid determination method | |
| CN101968422A (en) | Method for nondestructively detecting viscosity of nitro-cotton | |
| CN104458784B (en) | The degree of crystallinity of baccy fiber element and the assay method of crystalline structure | |
| CN111257403A (en) | Method for measuring stable hydrogen isotope ratio of pure acetic acid methyl site | |
| CN111141725A (en) | Quantitative detection method for lithium hexafluorophosphate in lithium ion battery electrolyte | |
| CN103674993A (en) | Method for measuring surface oil of natural protein fibers |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |