CN103323406B - A kind of fresh tea leaf fluorine content non-destructive rapid determination method - Google Patents
A kind of fresh tea leaf fluorine content non-destructive rapid determination method Download PDFInfo
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- CN103323406B CN103323406B CN201310220783.XA CN201310220783A CN103323406B CN 103323406 B CN103323406 B CN 103323406B CN 201310220783 A CN201310220783 A CN 201310220783A CN 103323406 B CN103323406 B CN 103323406B
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Abstract
A kind of fresh tea leaf fluorine content non-destructive rapid determination method, belongs to field of agricultural production technologies.It comprises the determination of SPAD value; The determination of Oil repellent; SPAD and Oil repellent dependent equation are determined; Content of Fluoride in Tea control criterion needed for reality is substituted into linear equation and draws the SPAD value controlled in Oil repellent standard; Carry out the harmless detection fast of SPAD value to blade when scene, field is plucked, the blade chosen within SPAD value range of control is plucked; When scene, field is plucked, the harmless detection fast of SPAD value is carried out to blade, substitute into SPAD and Oil repellent dependent equation, determine this blade Oil repellent.The present invention has practical directiveness on low fluorine fresh leaf is plucked, the scene that fresh leaf is not taking back Oil repellent in the situation of laboratory can be realized determine fast, overcome the drawback of first plucking in the past and detecting afterwards, achieve Sources controlling and real-time, detect then and there and pluck then and there, compensate for the many deficiencies existed in prior art simultaneously.
Description
Technical field
The invention belongs to field of agricultural production technologies, be specifically related to a kind of fresh tea leaf fluorine content non-destructive rapid determination method.
Background technology
Fluorine is distributed widely in occurring in nature, be one of trace element of needed by human, grow and play an important role in skeletal metabolism growth in humans, appropriate fluorine of taking in is intended healthy, but the Excess free enthalpy of fluorine can cause fluorosis, main manifestations is den tal fluorosis and fluorosis of bone.Tea tree is a kind of typical poly-fluorine plant, and the Oil repellent in tealeaves is than general plant height 1-2 order of magnitude.In actual production, control Content of Fluoride in Tea become one of key factor affecting quality and safety of tea.The accumulation of tea leaf fluorine and leaf growth time significant positive correlation, blade Oil repellent increases along with the increase of leaf age.
Current Content of Fluoride in Tea detects and mainly contains Fluoride ion selective electrode method, the chromatography of ions, efficient liquid phase liquid-liquid chromatography, spectrophotometric method etc.These methods are all be based upon to carry out adopting back on the tealeaves blade after drying or the one-tenth basis of sampling tea, namely Oil repellent is drawn by corresponding detecting instrument again after series of pretreatment being carried out to tealeaves dry sample, and on Sources controlling before harvesting, namely field Content of Fluoride in Tea detection method or indirectly defining method there is not yet report.
SPAD-502 is a kind of Handheld spectrometer, can in field Non-Destructive Testing plant leaf chlorophyll content, and the chlorophyll content of plant leaf blade increases along with the increase of leaf age, decreases to blade full maturity.Current chlorophyll meter is successfully applied in paddy rice, wheat, corn, lettuce, cotton, potatoes and other crops, tea tree fertilising also has corresponding utilization, but is not applied to Fluoride in Tea Related Research Domain.
Summary of the invention
For prior art Problems existing, the object of the invention is to design the technical scheme that a kind of fresh tea leaf fluorine content non-destructive rapid determination method is provided.
Described a kind of fresh tea leaf fluorine content non-destructive rapid determination method, is characterized in that comprising the following steps:
1) determination of SPAD value: blade field new tea slightly being gone up to differing maturity carries out the detection of SPAD value, draws the serial SPAD value of differing maturity blade;
2) determination of Oil repellent: synchronously get the blade having measured SPAD value and detect blade Oil repellent in the indoor conventional method of experiment, draw the serial Oil repellent detected value of differing maturity blade;
3) blade SPAD value step 1) recorded and step 2) the Oil repellent detected value that records carries out correlation analysis, draws SPAD and Oil repellent dependent equation;
4) linear equation that the Content of Fluoride in Tea control criterion substitution step 3) needed for reality draws is drawn the SPAD value controlled in Oil repellent standard;
5) carry out the harmless detection fast of SPAD value to blade when scene, field is plucked, the blade chosen within the SPAD value range of control drawn by step 4) is plucked, with from Sources controlling Content of Fluoride in Tea standard;
6) carry out the harmless detection fast of SPAD value to blade during the on-the-spot harvesting in field, the SPAD that substitution step 3) draws and Oil repellent dependent equation, determine this blade Oil repellent.
Described a kind of fresh tea leaf fluorine content non-destructive rapid determination method, is characterized in that gathering field new tea in described step 1) slightly goes up more than blade 100 leaf of differing maturity.
Described a kind of fresh tea leaf fluorine content non-destructive rapid determination method, is characterized in that the blade that in described step 5) and step 6), scene, field is plucked is that waiting of newly slightly going up plucks blade.
The present invention judges blade Oil repellent by measuring fresh tea leaf SPAD value, thus determine whether to meet Content of Fluoride in Tea standard, the method is reasonable in design, on low fluorine fresh leaf is plucked, there is practical directiveness, the scene that fresh leaf is not taking back Oil repellent in the situation of laboratory can be realized determine fast, overcome the drawback of first plucking in the past and detecting afterwards, achieve Sources controlling and real-time, detect then and there and pluck then and there, compensate for the many deficiencies existed in prior art simultaneously.
Accompanying drawing explanation
Fig. 1 is new tea slightly differing maturity blade SPAD value and Oil repellent correlativity figure.
Fig. 2 is new tea slightly blade SPAD value and Oil repellent correlativity figure.
Embodiment
The present invention is further illustrated below in conjunction with embodiment.
Experimental example 1
2012, production tea place is tested, the tea place new tea of amount of application of nitrogen fertilizer different under same land occupation condition is slightly carried out to the synchronous detection of SPAD and F content, during detection, choose the blade of Different Leaf Age, for determining leaf age value in this test, test adopts the method for hanging label mark to carry out.
Test is carried out on the tea place devising different amount of application of nitrogen fertilizer process, and use total amount tea place nitrogenous fertilizer year and be respectively 0,119,285,474,569 and 712kgN/ha, each nitrogen fertilizer amount respectively has 4 communities, and test amounts to 24 communities.Blade sampling to only growing to 5-6 leaf, namely being hung the leaf age signing blade and being respectively 8,15,22,29 and 36 days from just exhibition is after one week.Carry out SPAD value with SPAD-502 instrument to fresh leaf to detect, fetch after pulverizing is dried in laboratory simultaneously and measure blade Oil repellent by Fluoride ion selective electrode method.
Test obtains blade Oil repellent and each 183 data of SPAD value altogether, and carry out correlation analysis to its Oil repellent and SPAD value, result as shown in Figure 1.Test findings shows, different amount of application of nitrogen fertilizer tea places new tea is slightly gone up between fresh leaf blade SPAD value and blade Oil repellent exists extremely significant correlationship (R=0.854), and its dependent equation is
.
Assuming that fresh tea leaf fluorine standard of plucking is 300mg/kg, according to linear equation
calculating SPAD value is 63.4.The horizontal tea place of different nitrogen application measures to choose the blade of SPAD value within 63.4 some, take back use for laboratory fluoride ion selective electrode and detect its Oil repellent, the blade that Simultaneously test chooses SPAD value more than 63.4 is some for detecting Oil repellent, and it is as shown in the table for testing result.
Result shows (table 1), when blade SPAD value is controlled within about 63.4, different nitrogen fertilizer amount tea places blade Funing tablet average all within 300mg/kg, and SPAD value higher than 63.4 time its Funing tablet mean value at more than 300mg/kg.
Table 1: different nitrogen application tea places SPAD value and F concentration
Therefore, in actual production, this method available carries out SPAD and the Oil repellent detection of differing maturity blade to target tea place, calculates Oil repellent and SPAD value dependent equation.When plucking in field, scene is harmless to be detected fast to utilize SPAD-502 to carry out tealeaves blade, thus determines whether the new slightly blade of tealeaves meets Content of Fluoride in Tea standard of plucking.
Experimental example 2
In May, 2013, another production tea place is tested, pluck first expanded leaves, the second leaf, the 3rd leaf, the 4th leaf, the 5th leaf and totally 100, the 6th leaf, by SPAD-502 on-site measurement SPAD value.After taking back laboratory, dry and pulverize, measure the Oil repellent of every sheet leaf with fluoride ion selective electrode respectively.Analyze the correlativity between blade SPAD value and Oil repellent, result shows to exist extremely significant correlationship (R=0.595) between the two, and dependent equation is
(Fig. 2).
On tea place, random acquisition newly slightly goes up the 3rd leaf, the 4th leaf, the 5th leaf and the 6th leaf, measure SPAD value, calculate Content of Fluoride in Tea according to SPAD value and equation, and utilize ion selecting electrode determining Oil repellent, to verify equation accuracy, result is as shown in table 2.Result shows that gap between the Oil repellent that calculates according to equation monolithic leaf and actual Oil repellent is between 1.71-20.5%, what this test was set up passes through first to draw SPAD and Oil repellent dependent equation, thus SPAD-502 Fast Measurement SPAD value is utilized when field, by the calculating of equation, quick nondestructive can be carried out at scene, field to Content of Fluoride in Tea and determine.
Table 2 field gathers blade Oil repellent actual value and equation calculated value
Claims (1)
1. a fresh tea leaf fluorine content non-destructive rapid determination method, is characterized in that comprising the following steps:
1) determination of SPAD value: gather blade that field new tea slightly go up differing maturity and more than totally 100 leaves carry out the detection of SPAD value, draw the serial SPAD value of differing maturity blade;
2) determination of Oil repellent: synchronously get the blade having measured SPAD value and detect blade Oil repellent in the indoor conventional method of experiment, draw the serial Oil repellent detected value of differing maturity blade;
3) blade SPAD value step 1) recorded and step 2) the Oil repellent detected value that records carries out correlation analysis, draws SPAD and Oil repellent dependent equation;
4) linear equation that the Content of Fluoride in Tea control criterion substitution step 3) needed for reality draws is drawn the SPAD value controlled in Oil repellent standard;
5) wait that plucking blade carries out the harmless detection fast of SPAD value to what newly slightly go up when scene, field is plucked, the blade chosen within the SPAD value range of control drawn by step 4) is plucked, with from Sources controlling Content of Fluoride in Tea standard;
6) carry out the harmless detection fast of SPAD value to blade during the on-the-spot harvesting in field, the SPAD that substitution step 3) draws and Oil repellent dependent equation, determine this blade Oil repellent.
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| CN105675540A (en) * | 2016-01-15 | 2016-06-15 | 中国农业科学院茶叶研究所 | Rapid nondestructive determination method of aluminum content of fresh tea leaves |
| CN112213451B (en) * | 2020-10-21 | 2021-07-06 | 华南农业大学 | Nondestructive testing method for maturity of pseudobulb of dendrobium nobile and application thereof |
| CN114166956A (en) * | 2021-10-28 | 2022-03-11 | 广西壮族自治区林业科学研究院 | Method for rapidly detecting phlorizin and trilobatin in leaves of lithocarpus polystachyus rehd |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101507455A (en) * | 2009-02-07 | 2009-08-19 | 宜宾市外贸金叶茶业有限责任公司 | Production method of low-fluorine border-sale tea |
| CN101514959A (en) * | 2009-03-26 | 2009-08-26 | 中国农业科学院茶叶研究所 | Nondestructive rapid determining and controlling method for nitrogen nutrition of tea plant |
| KR20100001401A (en) * | 2008-06-27 | 2010-01-06 | 대한민국(관리부서:농촌진흥청) | Non-destructive analysis method of fresh tea leaves by near infrared spectroscopy |
| CN102352253A (en) * | 2011-07-27 | 2012-02-15 | 中国农业科学院茶叶研究所 | Soil amendment and method for reducing fluorine content of fresh brick tea leaves |
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Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
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| KR20100001401A (en) * | 2008-06-27 | 2010-01-06 | 대한민국(관리부서:농촌진흥청) | Non-destructive analysis method of fresh tea leaves by near infrared spectroscopy |
| CN101507455A (en) * | 2009-02-07 | 2009-08-19 | 宜宾市外贸金叶茶业有限责任公司 | Production method of low-fluorine border-sale tea |
| CN101514959A (en) * | 2009-03-26 | 2009-08-26 | 中国农业科学院茶叶研究所 | Nondestructive rapid determining and controlling method for nitrogen nutrition of tea plant |
| CN102352253A (en) * | 2011-07-27 | 2012-02-15 | 中国农业科学院茶叶研究所 | Soil amendment and method for reducing fluorine content of fresh brick tea leaves |
Non-Patent Citations (1)
| Title |
|---|
| 氟对茶树生长、叶片生理生化指标与茶叶品质的影响;唐茜等;《植物营养与肥料学报》;20111231;第17卷(第1期);第186-194页 * |
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