CN104611247A - Application of Alcaligenes faecalis sp. (DQP3) in degradation of phenol and quinoline - Google Patents
Application of Alcaligenes faecalis sp. (DQP3) in degradation of phenol and quinoline Download PDFInfo
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Abstract
The invention relates to an Alcaligenes faecalis sp. DQP3 strain and its application in degradation of phenol and quinoline double substrates. 16srna identifies that the strain belongs to Alcaligenes faecalis sp, and has a preservation number of CGMCC No:9200. By optimizing the culture conditions, when the inoculated pathogen quantity is 10%, the medium initial pH is 7.0, the culture temperature is 35DEG C, and the oscillator rotation speed is 150r/min, the DQP3 has the highest degradation efficiency on phenol and quinoline in one substrate. Adding proper amounts of glucose (100mg/L) and NH4NO3 (200mg/L) as the external C source and N source into the medium can promote the degradation rate of DQP3 on phenol and quinoline, and when quinoline serves as the carbon source, the degradation efficiency is higher than that when it serves as the nitrogen source. Immobilized carrier screening test research shows that 1g/dL PAC, 7g/dL PVA and 3g/dL SA made into PVA-SA-PAC gel beads to serve as the carrier for fixation of DQP3 can reach the best removal effect on phenol and quinoline in wastewater. The concentration of 300mg/L quinoline can be decreased by 70% within 9h, and the concentration of 500mg/L phenol can be decreased by 80% within 24h.
Description
Invention field
The present invention relates to strain Bacillus foecalis alkaligenes Alcaligenes faecalis sp. (DQP3) in the application of degrading in same matrix in phenol, quinoline and its immobilized research
Background technology
Coking chemical waste water contains aromatics and heterogeneous ring compound, is the more typical trade effluent of a class that produces in the process of high temperature carbonization, coke refining, gas purification, byproduct processing and smart product purification.The composition of coking chemical waste water is more, and wherein organic constituents is complicated and toxicity is comparatively large, and therefore organic Environmental capacity is a process difficult problem for coking chemical waste water, constrains the water quality reaching standard after process, is Recent study focus.
What in the organic constituents of coking chemical waste water, proportion was the highest is phenol and its derivatives, and the content of quinoline and its derivates occupies second.Water-soluble and the volatility of phenol and quinoline is better, easily causes water body and topsoil, also has certain carcinogenesis to organism.If by the discharge of wastewater containing phenol and quinoline in environment, bring serious harm can to the ecosystem and human health.Therefore, the Degradation studying phenol and quinoline in coking chemical waste water is crucial.
Applicant screens the bacterial strain of a highly effective degrading phenol, quinoline from Shoudu Iron and Steel Co coking chemical waste water second pond mud, and be accredited as Bacillus foecalis alkaligenes Alcaligenes faecalis sp. through 16SrDNA did not also have relevant report in degradation condition, degradation rate, immobilization.This bacterium has good degradation capability for phenol, quinoline in same matrix after deliberation, has good application prospect.
Summary of the invention
The present invention relates to the application of a strain Alcaligenes faecalis Alcaligenes faecalis sp. bacterium in degraded is containing phenol, quinoline double-basis matter in phenol, quinoline, called after DQP3, this bacterial strain has good degradation of phenol, the ability of quinoline.This bacterial strain is preserved in China Committee for Culture Collection of Microorganisms of Institute of Microorganism, Academia Sinica common micro-organisms center, No. 3, Yard 1, BeiChen xi Road, Chaoyang District, Beijing City on May 26th, 2014, and its deposit number is CGMCC No:9200.
Through optimum culture condition, when connecing that bacterium amount is 10%, the initial pH of substratum is 7.0, culture temperature is 35 DEG C, vibrator rotating speed is 150r/min, DQP3 degrades the most effective of phenol and quinoline pollutent in same matrix.
When adding appropriate glucose (100mg/L) and NH4NO3 (200mg/L) as additional C source and N source in substratum, can promote the degradation rate of DQP3 Pyrogentisinic Acid and quinoline, and degradation efficiency during using quinoline as carbon source is higher as efficiency during nitrogenous source than it.
The research of fixation support shaker test shows that the polyvinyl alcohol (PVA) of PAC, 7g/dL with 1g/dL and the sodium alginate (SA) of 3g/dL are made PVA-SA-PAC gelled pill and fixed DQP3 as carrier, best to the removal effect of phenol in wastewater and quinoline.In 9h, the quinoline concentration of 30//0mg/L is reduced in 70%, 24h and the phenol of 500mg/L can be reduced by 80%.
Accompanying drawing explanation
Fig. 1 is the phylogeny tree graph of bacterial strain DQP3
Fig. 2 is the impact of inoculum size on DQP3 degradation capability
Fig. 3 is the impact of initial pH on the mono-stroma contaminants degradation rate of DQP3
The impact of Fig. 4 to be pH on DQP3 degrade phenol in same matrix, quinoline-degrading rate
Fig. 5 is temperature on the impact of DQP3 phenol and quinoline-degrading rate
Fig. 6 is the impact of rotating speed on DQP3 phenol, quinoline-degrading rate
Fig. 7 is that different initial substrate concentration is on the impact of DQP3 degradation capability
Fig. 8 is DQP3 thalli growth and phenol degrading characteristic under different initial phenol concentration
Fig. 9 is DQP3 thalli growth and quinoline-degrading characteristic under different initial quinoline concentration
Figure 10 is the impact of different glucose concn on DQP3 degraded quinoline
Figure 11 is the impact of different N H4NO3 concentration on DQP3 degraded quinoline
The impact that Figure 12 is additional C source, quinoline is degraded in N source on DQP3
Figure 13 is that additional C source, N source are on the impact of DQP3 degradation of phenol
Figure 14 is different fixation support Pyrogentisinic Acids and the removal effect of quinoline
Embodiment
Following examples for illustration of the present invention, but are not used for limiting the scope of the invention.
The screening of embodiment 1 bacterial strain
1, bacterium source and enrichment
The second pond mud 5mL getting Shoudu Iron and Steel Co disposal of coking plant waste water system adds 50mL extractum carnis liquid nutrient medium, and adds phenol and quinoline wherein and make the two concentration be respectively 100mg/L and 50mg/L.Put into constant temperature oscillator 30 DEG C, 150r/min, cultivate 3d, obtain enrichment culture liquid.
2, the screening of bacterial strain
The nutrient solution of enrichment is got 5mL and put into 10mL centrifuge tube at high speed freezing centrifuge 10000r/min, 23 DEG C, centrifugal 5min.Get precipitation add physiological saline mixing after centrifugal, repeat twice, to remove remaining medium and meta-bolites.Carry out domestication cultivation in the precipitation thalline access minimal medium obtained centrifugal, in substratum, the starting point concentration of phenol is 50mg/L, the starting point concentration of quinoline is 25mg/L.Domestication, improves concentration gradually, when concentration reaches to phenol 500mg/L, and quinoline 250mg/L.Shi Jinhang plate streaking isolated strains.
Minimal medium: NaCl 0.5g, K
2hPO
40.5g, KH
2pO
40.5g, MgSO
40.5g, distilled water 1000mL, pH are 7.0
3, the multiple sieve of bacterial strain
Some bacterial strains of domestication gained all can grow on the substratum taking phenol and quinoline as uniquely jointly carbon source, by the size of bacterium colony and distribution number pick out the different strain number DQP1-DQP4 of 4 strain forms.The nutrient solution of bacterial strain DQP1-DQP4 is respectively got respectively in the 90mL minimal medium that 10mL bacteria suspension joins respectively containing 200mg/L phenol and 150mg/L quinoline two kinds of pollutents, guarantee the initial OD of every bottle of mixed solution
600identical, cultivate in isothermal vibration incubator, after 24h, measure the degraded situation of four kinds of single bacterium Pyrogentisinic Acids and quinoline respectively.Filter out and the phenol in same matrix and the highest strain bacterium of quinoline-degrading ability are preserved, for follow-up study.
The qualification of embodiment 2 bacterial strain
Use Solarbio bacterial genomes DNA extraction kit, extract the genomic dna of bacterial strain, as template, utilize 16SrDNA gene universal primer, carry out pcr amplification.The primer of amplification 16SrDNA:
F-primerF27：5’-AGA GTT TGA TCC TGG CTC AG-3’
R-primerR1492：5’-GGC TAC CTT GTT ACG ACT T-3’
PCR reaction system and condition:
PCR reaction conditions is specifically set to: 94 DEG C of denaturation 5 min, 94 DEG C of sex change 1 min, 55 DEG C of annealing 30 s, and 72 DEG C extend 90 s, 40 circulations, and 72 DEG C finally extend 5 min, 4 DEG C of preservations.The PCR reaction product of getting 2 μ L carries out the agarose gel electrophoresis experiment of 1%.
The 16SrDNA sequence product obtained is checked order by Shanghai Sangon Biological Engineering Technology And Service Co., Ltd.The sequence recorded logs in NCBI website, is compared by Blast program and Genbank amplifying nucleic acid data.Use clustalx, MEGA3 Software on Drawing bacterial strain evo-devo tree (see accompanying drawing 1).
The top condition screening that embodiment 3 bacterial strain DQP3 degrades
The condition affecting bacterial growth mainly contains temperature, pH value, shaking speed, bacterial load etc.
1. inoculum size is on the impact of DQP3 degradation capability
DQP3 inoculum size is respectively 5%, 8%, 10% three components does not access the inorganic salt liquid substratum containing 100mg/L phenol, 50mg/L quinoline, and initial pH 7.0 obtains the impact of different vaccination amount on DQP3 degradation capability.Result is see accompanying drawing 2
Inoculum size to degrade in same substratum phenol and quinoline Pollutant effects as shown in Figure 2 to DQP3.Can be degradable by phenol after the inoculum size 24h of 10%, to the degradation rate of quinoline also up to 80%, 2.5 times of degradation rate when being all about 5% inoculum size.
The impact that 2.pH degrades on DQP3
5,7,9,11 are adjusted to respectively containing the sole carbon source initial pH value of medium value that phenol concentration is 150mg/L with DQP3 inoculum size 10% access.Similar, to study quinoline under different pH be sole carbon source concentration is 100mg/L.Show that degradation of phenol and quinoline are the optimum pH scope of the substratum of unique energy source to DQP3 respectively.Result is see accompanying drawing 3
The degradation capability of DQP3 Pyrogentisinic Acid affects comparatively large by pH, peracid or excessively alkali all can make the degradation rate of phenol obviously reduce.After 24h, pH is the degradation rate nearly 100% of the experimental group phenol of 7 and 9, and pH is that in the experimental group of 5 and 11, phenol degrading rate is lower than 50%, and when wherein pH is 11, degradation rate is lower, is less than 20%.When therefore drawing DQP3 degradation of phenol, pH scope should control at 7-9.Affect more weak in DQP3 degraded quinoline process by pH, under neutral and weak acid, weak basic condition, it is all higher to the degradation efficiency of quinoline, and difference is little.And pH crosses alkali (pH=11) that DQP3 can be suppressed the degradation capability of quinoline, after 24h, about 30% is only to the degradation rate of 100mg/L quinoline, far below at pH be 5-7 condition under the degradation rate (being about 80%) of quinoline.Therefore, DQP3 is when degrading quinoline, and pH should control in the scope of 5-9.
Degraded respectively two kinds by DQP3 and pollute experimental studies have found that of optimal pH, pH scope when the independent degradation capability of DQP3 to two kinds of pollutents is higher is at 5-9.Add two kinds of pollutents at minimal medium, make that the concentration of Phenol in Aqueous Solution is 200mg/L, the concentration of quinoline is 100mg/L.The initial pH of regulator solution is respectively 6,7,8,9 (each pH establishes three parallel laboratory tests to average), draws the degradation rate of the two.Result is see accompanying drawing 4.
DQP3 degrade phenol, quinoline pollutent in same matrix time optimum PH range should be 7 ~ 9.But as pH > 8, the substratum of preparation before sterilization after all can present muddiness, there is white precipitate, this is because medium component can react in the basic conditions generate insoluble white precipitate.This kind of phenomenon can reduce the nutrition content of substratum, and improves the turbidity of water sample, the mensuration to pollutant load after impact sampling.In addition, the difference of pH also can affect the color of substratum in the process of DQP3 degradation of contaminant.Comprehensive above-mentioned conclusion and analysis, in following research, all select pH=7.0 to be that DQP3 degrades the optimal pH of phenol in same matrix, quinoline, allowable fluctuation range is 7.0 ~ 7.5.
3. temperature is on the impact of DQP3 degradation capability
With 300mg/L phenol, 150mg/L quinoline configuration minimal medium, rear access DQP3, sets different temperature (25 DEG C, 30 DEG C, 35 DEG C, 40 DEG C, 45 DEG C) and obtains temperature to the impact of degradation efficiency.Result is see accompanying drawing 5.
The efficiency of temperature to DQP3 degradation of phenol, quinoline also has certain influence, but does not have the impact of pH and inoculum size remarkable.Within the scope of 30 DEG C ~ 40 DEG C, the degradation rate of phenol is higher, is about about 80%; And the degradation rate (< 60%) of quinoline is starkly lower than the degradation effect (80%) under the condition of 30 DEG C ~ 35 DEG C when 40 DEG C.35 DEG C time, the degradation rate of phenol and quinoline all reaches maximum value.And two kinds of contaminant degradation rates when 25 DEG C are all lower than 40 DEG C, higher than 45 DEG C.
4. vibrator rotating speed is on the impact of DQP3 degradation capability
With 300mg/L phenol, 150mg/L quinoline configuration minimal medium, rear access DQP3, setting speed is 50rad/min, 70 rad/min, 100 rad/min, 150 rad/min and 200 rad/min respectively, obtains the impact of rotating speed on degradation efficiency.Result is see accompanying drawing 6
Rotating speed is within the scope of 50 ~ 150rad/min, and along with the raising of rotating speed, the degradation rate also corresponding raising of DQP3 Pyrogentisinic Acid and quinoline, presents linear relationship substantially.Under rotating speed is 150rad/min, the degradation capability of DQP3 is maximum, the degradation rate of Pyrogentisinic Acid nearly 90% after 24h, to the degradation rate of quinoline also higher than 70%.Further raising rotating speed, the degradation rate of phenol and quinoline will no longer improve, and start on the contrary significantly to reduce, the degradation rate under 100rad/min condition.In the test of DQP3 degradation of phenol and quinoline, the speed setting of vibrator is that 150rad/min is comparatively suitable.
5. concentration of substrate is on the impact of DQP3 degradation capability
With different concns only containing phenol minimal medium (300mg/L, 500 mg/L, 700 mg/L, 900 mg/L) with only contain quinoline minimal medium (200 mg/L, 300 mg/L, 400 mg/L, 500 mg/L) and access DQP3 respectively for the substratum of substrate.Obtain substrate to the impact of degradation capability.Result is see accompanying drawing 7
The impact of the time contaminated substrate concentration needed for the degradable pollutent of DQP3 is comparatively large, and the concentration of pollutent is lower, then by DQP3 degradable time of consuming shorter.In 24h, DQP3 can the phenol of degradable 300 mg/L concentration, is about 60% to the degradation rate of the phenol of 500mg/L concentration, but can phenol below degradable 500mg/L concentration in 36h.When phenol concentration is more than 700 mg/L, the degradation efficiency of DQP3 obviously reduces the degradation rate of Pyrogentisinic Acid in 24h and is only about 30%.During 36h, concentration is that the degradation rate of 700mg/L phenol is up to 80%.When phenol concentration increases to 900mg/L further, the activity of DQP3 is obviously suppressed, and the degradation rate in 48h is all lower, all below 10%.The concentration limit of DQP3 energy efficient degradation phenol in 48h is 700mg/L.
The time of DQP3 degraded needed for quinoline extends along with the increase of quinoline concentration.Concentration is that the degradation rate of quinoline in 24h of 200mg/L reaches 90% rapidly, but less to the amplification of its degradation rate in 48h after 24h.The degradation rate of quinoline when 24h that concentration is respectively 300mg/L and 400mg/L then reduces to about 50%, but after 24h the degradation rate speedup of quinoline remain unchanged straight line rise, during 36h, the quinoline-degrading rate of 300mg/L reaches 90%.When quinoline concentration is 500mg/L, be degraded hardly in front 24h, the speedup that degradation rate during 36h is only degradation rate after 40%, 36h is slow, and degradation rate during 48h is less than 45%.Therefore, the concentration limit of DQP3 efficient degradation quinoline in 48h is 400mg/L.The Utilization ability of the single matrix of DQP3 Pyrogentisinic Acid is obviously better than quinoline.Thalline can the concentration of degradation of phenol at short notice higher than the concentration of quinoline.
The external carbon nitrogen source of embodiment 4 is on the impact of quinoline-degrading
The relation of the independent degradation of phenol of 1.DQP3, quinoline and self thalli growth
Set different initial phenol concentration (being respectively 100mg/L, 200 mg/L, 300 mg/L, 400 mg/L, 500 mg/L, 600 mg/L, 700 mg/L, 800 mg/L and 900mg/L) and different quinoline starting point concentration (being respectively 50mg/L, 80 mg/L, 130 mg/L, 200 mg/L, 300 mg/L, 400 mg/L, 500 mg/L, 600 mg/L and 700mg/L).The DQP3 of access logarithmic phase cultivates under best degradation condition.Measure the concentration of thalline (with OD
600represent) and phenol, quinoline degraded situation, there is the relation of degradation of substrates and DQP3 bacterial strain own growth in situation in the substrate of investigation different concns.Experimental result is with reference to accompanying drawing 8,9.
Along with the increase of phenol concentration, the time that DQP3 logarithmic phase occurs postpones more thereupon.Obvious restraining effect can not be produced to DQP3 bacterial strain when the concentration of phenol is at 200 below mg/L; in the lag phase of DQP3 growth, phenol concentration reduces hardly; when after the logarithmic phase entering thalli growth, phenol concentration declines rapidly; and after bacterial growth enters stationary phase; phenol concentration reduction then becomes slowly, even no longer reduces.Bacterium can be degraded rapidly the phenol of lower concentration, but the time needed for degradable phenol can extend with the increase of Phenol in Aqueous Solution concentration, and through after a period of time finally can be degradable, phenol concentration is higher, and to be completely degraded the required time also longer.But when concentration is higher than 700mg/L, before the growth arrival logarithmic phase of DQP3, phenol is degraded hardly, just start rapid degraded, but degradation rate is less than the degradation rate of Low Concentration Phenol after reaching logarithmic phase.
When the concentration of quinoline is within the scope of 130mg/L, the growth lag phase of DQP3 thalline is very of short duration, logarithmic phase can be entered at short notice, when 12h, DQP3 reaches the stable growth phase, biological accumulation amount now in solution is also maximum, quinoline is during this period by fast degradation, almost degradable in 12h.Time required for the degradable quinoline of DQP3 is significant prolongation along with the increase of quinoline concentration, degradable concentration is that the quinoline of 400mg/L needs 48h, concentration is that the quinoline of 500mg/L is completely degraded not yet when 72h, and when quinoline concentration is enlarged to 600mg/L further in 72h degradation rate be only 20%.Under 700mg/L concentration, the toxicity of quinoline is excessive, not only suppresses the growth of DQP3, and even make DQP3 pellet fraction dead, the quinoline therefore under this concentration is not almost degraded.Can draw thus, the quinoline in low strength range can be completely degraded along with the prolongation of time, and the degraded that concentration increases quinoline is then incomplete, and during excessive concentration (being greater than 700mg/L), quinoline is degraded hardly.
The experimental result of comprehensive degradation of phenol and quinoline is separately known, and the Degradation of DQP3 bacterial strain Pyrogentisinic Acid and these two kinds of pollutents of quinoline mainly occurs in the logarithmic phase of thalli growth, and concentration of substrate is higher, reaches the OD of bacterium after stationary phase
600be worth also higher.When concentration of substrate is lower, the growth of DQP3 bacterial strain is promoted, and the degradation efficiency of substrate is also higher.The excessive concentration of substrate then can suppress growth and the degradation capability of DQP3, and concentration of substrate is higher, to the growth of bacterial strain and the restraining effect of degradation capability stronger, thalline can be made to lose degradation capability and dead higher than certain limit.
2. additional carbon, nitrogenous source are on the impact of DQP3 degradation capability
1. additional C, N source of different concns is on the impact of DQP3 degraded quinoline ability
The glucose arranging different concns is the NH of external carbon source, different concns
4nO
3external nitrogenous source, accessing quinoline concentration is respectively the minimal medium of 400mg/L, and rear access bacterial strain DQP3 cultivates.Experimental result is with reference to accompanying drawing 10,11.
Not add C source for contrast, when glucose concn is less than 100mg/L, the efficiency of DQP3 degraded quinoline improves with the increasing of glucose concn.When additional glucose concn is 100mg/L, maximum to the promoter action of DQP3 degraded quinoline, the degradation rate in 24h is higher than 60%.When glucose concn higher than 100mg/L along with the increase promoter action of glucose concn weakens gradually, when glucose concn reaches 800mg/, DQP3 almost no longer degrades quinoline.By within the scope of the known 500mg/L of thalli growth curve, glucose concn is higher, and the final biomass of thalline is larger.But the biomass of more than 800mg/L thalline increases hardly.To sum up can obtain, as additional C source, the glucose adding 100mg/L in substratum can promote that DQP3 is to the Degradation of quinoline preferably.
Compared with control group, additional N source can promote that DQP3 is to the Degradation of quinoline greatly.Work as NH
4nO
3when concentration is within the scope of 0 ~ 1000mg/L, DQP3 increases with the rising of N source concentration the degradation capability of quinoline.As NH in solution
4nO
3when concentration is 1000mg/L, the degradation effect of quinoline is best, and the degradation rate in 24h, up to 90%, is 3 times of control group.But NH4NO3 concentration is higher than after 200mg/L, the promoter action difference of additional N source to quinoline-degrading is little.Therefore, the NH adding 200mg/L in minimal medium is selected
4nO
3to promote that DQP3 is to the degraded of quinoline.
Be respectively C source and N source all not add (control group), only add C source, only add N source and add C source and N source simultaneously, accessing quinoline concentration is respectively the minimal medium of 400mg/L, and rear access bacterial strain DQP3 cultivates.Experimental result is with reference to accompanying drawing 12.
When without external glucose and ammonium nitrate, the quinoline concentration in solution can be reduced to below 50mg/L by DQP3 in 40h; Illustrate that quinoline can provide Carbon and nitrogen sources for the growth of DQP3 bacterial strain, metabolism.When glucose and ammonium nitrate exist simultaneously, bacterial strain DQP3 can degrade quinoline but degradation rate is now minimum in four groups of experiments.Additional C source and the amount in N source too much, inhibit DQP3 to the degraded of quinoline, and bacterium is selected preferentially to utilize additional C source and N source to carry out growth metabolism, and after the concentration of thalline in solution increases, quinoline is just degraded rapidly.When only having additional carbon in minimal medium or only have additional nitrogenous source to exist, bacterial strain DQP3 can only utilize quinoline as the required energy of growth, and therefore these two groups experiments can accelerate the degraded of DQP3 to quinoline.And quinoline is better as efficiency during nitrogenous source than it as degradation efficiency during carbon source.
2. additional C source, N source are on the impact of DQP3 degradation of phenol
By the known phenol of the molecular structure of phenol not containing atom N, under not having external N source existent condition, phenol cannot provide the required energy for the growth of DQP3, thus can not degrade by DQP3.The NH of different concns is set
4nO
3external nitrogenous source, accesses the minimal medium of the phenol of 600mg/L respectively, and rear access bacterial strain DQP3 cultivates.Experimental result is with reference to Figure 13.
Only add in substratum ammonium nitrate be additional N source and be unique C source with phenol time, the degradation efficiency of DQP3 Pyrogentisinic Acid is the highest.Therefore conclusion is obtained: only have and add NH in substratum
4nO
3during as additional nitrogenous source, DQP3 could degradation of phenol and as sole carbon source be used for thalli growth.
The different fixation support of embodiment 5 is on the impact of strain degradation ability
The majority of the dominant degradation bacteria of fixing coking chemical waste water adopts entrapping method at present, this experiment mainly selects corn cob, sponge, gelled pill, gel active charcoal bead, gel gauze and gel active charcoal gauze to be carrier, with free bacterium for control experiment, probe into the impact on the ability of phenol and quinoline in its same matrix of degrading after fixing DQP3 with different carriers.Cultivate in the minimal medium put into respectively with the immobilized DQP3 of different carriers and free DQP3 containing 400mg/L phenol, 250mg/ quinoline.Result is with reference to Figure 14
Except corn cob, other carriers are to the removal effect that all can improve Phenol in Aqueous Solution and quinoline after being fixed of DQP3.Wherein, PVA-SA-PAC mixed carrier to DQP3 be fixed make gelled pill after the removal ability of phenol, quinoline in same matrix is greatly improved.The concentration of Phenol in Aqueous Solution is down to 100mg/L by 400mg/L in 24h, and the clearance of phenol can reach 100% in 36h.Meanwhile, the biological activated carbon gelled pill after fixing can make the concentration of quinoline in solution be down to 70mg/L by 250mg/L in 12h, and clearance is more than 70%.Gac gelled pill is only second to for carrier is fixed the rear removal ability to Phenol in Aqueous Solution and quinoline to DQP3 with PVA-SA-PAC-gauze.The two is compared with gauze carrier with the gelled pill not adding gac, in 12h, the removal efficiency of Pyrogentisinic Acid improves more than one times, the clearance of quinoline is improved more than three times, illustrate with PVA-SA-PAC to be that carrier fixes the removal of free DQP3 for Wastewater Pollutant, in this process, the adsorption effect of gac is better than the degradation effect of microorganism.When sponge is as carrier, the removal efficiency of Pyrogentisinic Acid and quinoline is lower than the two groups of carriers adding gac, but higher than not adding two groups of gel carriers of gac, illustrate that sponge also has stronger adsorptive power because internal voids is many, the pollutent in adsorbable solution.But the absorption of sponge is only physical process, pressing sponge can make thalline pass back into solution together with the phenol adsorbed, quinoline, and two kinds of pollutant loads in solution can increase again, and stability is the poorest in several carrier, therefore do not select sponge to be carrier.And gac gauze is carrier, after cultivating for some time, find that solution starts to become muddy, supposition may be that gauze space is comparatively large, and gel soaks at field time and starts excessive after concussion, and this can affect the recycling of carrier and make water outlet muddy.Therefore the stability of comprehensive carrier and removal effect, it is best with PVA-SA-PAC to be finally that carrier embedding DQP3 thalline makes the method for gelled pill.
The above; be only the present invention's preferably embodiment, but protection scope of the present invention is not limited thereto, is anyly familiar with those skilled in the art in the technical scope that the present invention discloses; the change that can expect easily or replacement, all should be encompassed within protection scope of the present invention.
Claims (4)
1. be used for can the Gram-negative bacteria of simultaneously degradation of phenol and quinoline in a strain, called after DQP3, through being accredited as Alcaligenes faecalis, and preserving number: CCTCC NO.9200.
2. bacterial strain described in claim 1 is used for the application of simultaneously degradation of phenol and quinoline, this strains for degrading top condition: connect that bacterium amount is 10%, the initial pH of substratum is 7.0, culture temperature is 35 DEG C, vibrator rotating speed is 150r/min.
3. as claimed in claim 2 bacterial strain at the same time degradation of phenol and quinoline two in application, when its feature adds appropriate glucose (100mg/L) and NH4NO3 (200mg/L) as additional C source and N source, the degradation rate of DQP3 Pyrogentisinic Acid and quinoline can be promoted.
4. the application of bacterial strain at the same time in degradation of phenol quinoline as claimed in claim 2, its feature is used for the polyvinyl alcohol (PVA) of PAC, 7g/dL of 1g/dL and the sodium alginate (SA) of 3g/dL and makes PVA-SA-PAC gelled pill and fix DQP3 as carrier, in 9h, the quinoline concentration of 300mg/L is reduced in 70%, 24h and the phenol of 500mg/L can be reduced by 80%.
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