CN104593223A - Microalgae breeding device and microalgae breeding method - Google Patents
Microalgae breeding device and microalgae breeding method Download PDFInfo
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Abstract
The invention discloses a microalgae breeding device and a microalgae breeding method, relating to the technical field of microalgae breeding. According to the microalgae breeding device, while the light utilization efficiency of high-intensity lighting is improved, high mixing strength is not required, and the damage of shearing force, caused by high-strength mixing, to algal cells is further avoided; the microalgae breeding device comprises a photobioreactor which comprises at least two breeding layers, and an aeration device is arranged in each breeding layer or an aeration device and a stirring device are both arranged in each breeding layer. The microalgae breeding device and the microalgae breeding method are suitable for the microalgae breeding process.
Description
Technical field
The present invention relates to both culturing microalgae technical field, particularly relate to a kind of microalgae culturing device adopting and method.
Background technology
During microdisk electrode, the utilization ratio of micro-algae to light depends on the number of the light that frustule absorbs.The illumination of high strength is the precondition of high yield, but there is light saturation point to the absorption of light and utilization in frustule, when receiving the light intensity of this light saturation point, the utilising efficiency of frustule to light reaches the highest, if but frustule light utilising efficiency will be caused to decline when receiving the light intensity higher than this light saturation point, and too much luminous energy can lose with the form of heat and fluorescence, the cell lower for light saturation point even can cause damage.Therefore, in breeding process, in order to ensure that each frustule can be subject to the irradiation of light uniformly, need carry out aeration or be uniformly mixed, frustule is carried out " light and shade circulation " in cultivation system, namely constantly move having between light and dark, to meet the demand of frustule Photosynthetic, be unlikely to again to allow frustule for a long time or always light and produce light injury or produce temperature and cross high phenomenon thus make frustule impaired or dead simultaneously.
During microdisk electrode, in order to improve output, usual employing high-density breeding, irradiate the illumination of high strength simultaneously, in this culturing process in order to improve micro-algae to the utilization ratio of light, improve frustule and connect light uniformity coefficient, usually need higher mixture strength, as by raising ventilation ratio or improve stirring intensity etc., to avoid making frustule local or the high light intensity of long-time reception because of undermixing, thus serious impact is caused on algae cell activity.
But, although high-density breeding of the prior art can improve by improving mixture strength the light utilising efficiency that frustule connects light uniformity coefficient, raising high-intensity illumination, but, also there are the following problems for high-density culture technology of the prior art: (1) is according to the culture apparatus of narrow light path, cannot be fully used after light can be made to penetrate this culture apparatus, cause optical energy loss; If but make light not penetrate this culture apparatus, then need the cultivation density improving frustule, but the method can make cell density not easily mate with intensity of illumination and mixture strength: if undermixing, then frustule reception luminous energy is uneven, if mixed strong, the shearing force then produced can cause damage to frustule, and improves algae cell density the consumption of frustule respiration also can be made comparatively large, causes net production to decline; (2) according to the culture apparatus of wide light path, the movement path of frustule is longer, be unfavorable for that frustule receives luminous energy fast and carries out photosynthesis, wide light path is often along with the mixing demand of high strength simultaneously, energy consumption is larger, and the shearing force that high strength mixing produces also can cause damage to frustule, affects the lifting of output.
Therefore, although high-density culture technology of the prior art can improve the utilising efficiency of high-intensity illumination, also exist mixture strength demand high, frustule can be made to cause the problems such as damage; If but ensure that mixture strength lower being unlikely to of frustule causes damage, then cannot make full use of high-intensity illumination again, can cause optical energy loss, light utilising efficiency reduces.
Summary of the invention
Main purpose of the present invention is, a kind of microalgae culturing device adopting and method are provided, can, while the light utilising efficiency improving high-intensity illumination, be unlikely to again to need higher mixture strength, and then the damage that the shearing force avoiding high strength to mix bringing causes frustule.
For achieving the above object, the present invention adopts following technical scheme:
On the one hand, the invention provides a kind of microalgae culturing device adopting, comprising: bioreactor, described bioreactor comprises at least two cultivation layers, is equipped with breather, or is equipped with breather and whipping appts in each described cultivation layer.
Alternatively, described bioreactor comprises at least two superimposed cultivation layers; Or,
Described bioreactor comprises at least two cultivation layers be nested.
Particularly, the thickness cultivating layer described in each is equal; Or
Second side extremely relative with described first side by the first side of described bioreactor, the thickness of at least two described cultivation layers increases successively; Or
By outside to the inside of described bioreactor of described bioreactor, the thickness of at least two described cultivation layers increases successively.
Further, the bottom of described bioreactor is provided with swivel arrangement.
On the other hand, the embodiment of the present invention additionally provides a kind of both culturing microalgae method, employs the described microalgae culturing device adopting provided in above-mentioned arbitrary technical scheme, and described both culturing microalgae method comprises step:
1) according to the intensity of illumination that each cultivation layer of described microalgae culturing device adopting receives, algae kind be seeded to described in each and cultivate in layer, wherein, the inoculum density cultivating layer described in each reduces with the reduction cultivating the intensity of illumination that layer receives described in each;
2), in breeding process, pass into gas to cultivating in layer described in each, wherein, the ventilation ratio cultivating layer described in each with cultivate described in each layer inoculum density reduction and reduce.
Particularly, when the two sides of described microalgae culturing device adopting all receives illumination and/or when described bioreactor comprises at least two cultivation layers be nested, described both culturing microalgae method specifically comprises:
1) by outside to the inside of described bioreactor of described bioreactor, the intensity of illumination that at least two described cultivation layers receive weakens successively; Algae kind being seeded to described in each cultivates in layer, and wherein, by outside to the inside of described bioreactor of described bioreactor, the inoculum density of at least two described cultivation layers reduces successively;
2) in breeding process, pass into gas to cultivating in layer described in each, wherein, by outside to the inside of described bioreactor of described bioreactor, the ventilation ratio of at least two described cultivation layers reduces successively;
When described microalgae culturing device adopting from one side receive illumination and described bioreactor comprises at least two superimposed cultivation layers time, described both culturing microalgae method specifically comprises:
1) by the first side to second side relative with described first side of described bioreactor, the intensity of illumination that at least two described cultivation layers receive weakens successively; Algae kind being seeded to described in each cultivates in layer, wherein, second side extremely relative with described first side by the first side of described bioreactor, the inoculum density of at least two described cultivation layers reduces successively;
2) in breeding process, pass into gas to cultivating in layer described in each, wherein, second side extremely relative with described first side by the first side of described bioreactor, the ventilation ratio of at least two described cultivation layers reduces successively.
In addition, step 1) before, described method also comprises:
Arrange described bioreactor each described in cultivate the thickness of layer all equal; Or,
When the two sides of described microalgae culturing device adopting all receive illumination and/or when described bioreactor comprise at least two be nested cultivation layer time, by outside to the inside of described bioreactor of described bioreactor, the thickness arranging at least two described cultivation layers increases successively;
When described microalgae culturing device adopting from one side receive illumination and described bioreactor comprises at least two superimposed cultivation layers time, second side extremely relative with described first side by the first side of described bioreactor, the thickness arranging at least two described cultivation layers increases successively.
Further, in breeding process, when described microalgae culturing device adopting from one side receive illumination and described bioreactor comprises at least two superimposed cultivation layers time, step 2) also comprise: the sensitive surface adjusting described bioreactor towards, the intensity of illumination that the first side joint of described bioreactor is received is greater than the intensity of illumination that described second side joint receives always.
Alternatively, described illumination comprises artificial lighting or natural lighting, and described intensity of illumination is more than or equal to 500 μm of ol/m
2/ s.
Alternatively, described ventilation ratio is 0.1 ~ 10vvm.
The microalgae culturing device adopting that the embodiment of the present invention provides and method, comprise bioreactor, and this bioreactor comprises at least two cultivation layers, during both culturing microalgae, the intensity of illumination that can receive according to each cultivation layer of this microalgae culturing device adopting, in each cultivation layer, inoculate the algae kind of different densities, and the inoculum density in each cultivation layer can be made to cultivate the reduction of the intensity of illumination that layer receives with each and reduce; In breeding process, can cultivate in layer respectively to each and pass into gas, the ventilation ratio of each cultivation layer is reduced with the reduction of the inoculum density of each cultivation layer; Like this, high-intensity illumination irradiates the cultivation layer of high cultivation density, and can be absorbed by the micro-algae in the cultivation layer of low cultivation density thus carry out photosynthesis through the illumination that the intensity of high cultivation density cultivation layer is more weak; Like this, the microalgae culturing device adopting entirety that the embodiment of the present invention provides improves the utilization ratio of high-intensity illumination, and then improves overall yield; And, for the cultivation layer that the intensity of illumination received is higher, cultivation density wherein and ventilation ratio are all higher, and for the lower cultivation layer of the intensity of illumination received, cultivation density wherein and ventilation ratio are all lower, compared to the mode adopting high-density breeding overall in prior art, reduce ventilation energy consumption, avoid high strength and mix the shear property model brought.
Accompanying drawing explanation
In order to be illustrated more clearly in the embodiment of the present invention or technical scheme of the prior art, be briefly described to the accompanying drawing used required in embodiment or description of the prior art below, apparently, accompanying drawing in the following describes is only some embodiments of the present invention, for those of ordinary skill in the art, under the prerequisite not paying creative work, other accompanying drawing can also be obtained according to these accompanying drawings.
A kind of microalgae culturing device adopting that Fig. 1 provides for the embodiment of the present invention;
The another kind of microalgae culturing device adopting that Fig. 2 provides for the embodiment of the present invention;
The another kind of microalgae culturing device adopting that Fig. 3 provides for the embodiment of the present invention;
The schema of a kind of both culturing microalgae method that Fig. 4 provides for the embodiment of the present invention.
Embodiment
Below in conjunction with the accompanying drawing in the embodiment of the present invention, be clearly and completely described the technical scheme in the embodiment of the present invention, obviously, described embodiment is only a part of embodiment of the present invention, instead of whole embodiments.Based on the embodiment in the present invention, those of ordinary skill in the art, not making the every other embodiment obtained under creative work prerequisite, belong to the scope of protection of the invention.
Embodiments provide a kind of microalgae culturing device adopting, comprising: bioreactor, this bioreactor comprises at least two cultivation layers, is equipped with breather, or is equipped with breather and whipping appts in each cultivation layer.
The microalgae culturing device adopting that the embodiment of the present invention provides, comprise bioreactor, and this bioreactor comprises at least two cultivation layers, during both culturing microalgae, the intensity of illumination that can receive according to each cultivation layer of this microalgae culturing device adopting, in each cultivation layer, inoculate the algae kind of different densities, and the inoculum density in each cultivation layer can be made to cultivate the reduction of the intensity of illumination that layer receives with each and reduce; In breeding process, can cultivate in layer respectively to each and pass into gas, the ventilation ratio of each cultivation layer is reduced with the reduction of the inoculum density of each cultivation layer; Like this, high-intensity illumination irradiates the cultivation layer of high cultivation density, and can be absorbed by the micro-algae in the cultivation layer of low cultivation density thus carry out photosynthesis through the illumination that the intensity of high cultivation density cultivation layer is more weak; Like this, the microalgae culturing device adopting that the embodiment of the present invention provides achieves making full use of of luminous energy, and entirety improves the utilization ratio of high-intensity illumination, and then improves overall yield; And, for the cultivation layer that the intensity of illumination received is higher, cultivation density wherein and ventilation ratio are all higher, and for the lower cultivation layer of the intensity of illumination received, cultivation density wherein and ventilation ratio are all lower, compared to the mode adopting high-density breeding overall in prior art, reduce ventilation energy consumption, avoid high strength and mix the shear property model brought.
It should be noted that, in the embodiment of the present invention, described ventilation ratio (air volume/culturevolume/min, vvm) refers to the ratio of per minute air flow and actual material liquid volume; In addition, in breeding process, the mixture strength of algae liquid is usual and ventilation ratio is linear, and therefore, in breeding process, the size of ventilation ratio can represent the size of mixture strength in cultivation layer usually.
Particularly, in microalgae culturing device adopting, its bioreactor can comprise at least two superimposed cultivation layers, and namely this bioreactor is at least two-layer superimposed form; As shown in Figure 1, this bioreactor 10 can comprise three superimposed cultivation layers, namely the first cultivation layer 11, second cultivates layer 12 and the 3rd cultivation layer 13, or, this bioreactor can also comprise the cultivation layer of two, four, five, six, seven, eight, nine, ten or more numbers, those skilled in the art specifically can select according to actually operating conditions such as cultivation scale, the intensities of illumination that uses, and the present invention is not construed as limiting this.
Or in microalgae culturing device adopting, its bioreactor also can comprise at least two cultivation layers be nested, and namely this bioreactor is at least two-layer form be nested; As shown in Figure 2 and Figure 3, this bioreactor 20 can comprise two cultivation layers be nested, i.e. the first cultivation layer 21 and the second cultivation layer 22, or, this bioreactor can also comprise three, four, five, six, seven, eight, nine, ten or more the cultivation layers be nested, those skilled in the art specifically can select according to actually operating conditions such as cultivation scale, the intensities of illumination that uses, and the present invention is not construed as limiting this.
Wherein, the thickness of each cultivation layer can be equal; Or as shown in Figure 1, by the first side 14 of bioreactor 10 to second side 15 relative with this first side 14, thickness L11, L12, L13 of at least two cultivation layers increase successively.Like this, when this microalgae culturing device adopting receives illumination from one side, when another side does not carry out illumination or carries out the irradiation system of the low light level, such as, when light source is located at the first side 14, by the first side 14 of bioreactor 10 to its second side 15, at least two cultivation layers 11, 12 and 13 intensities of illumination received weaken successively, at this moment, the first cultivation layer 11 can be made, second cultivation layer 12 and the 3rd inoculum density cultivated in layer 13 reduce successively, and, in breeding process, gas is passed in each cultivation layer, and ensure the first cultivation layer 11, the ventilation ratio of the second cultivation layer 12 and the 3rd cultivation layer 13 reduces successively.
That is, when microalgae culturing device adopting receives illumination from one side, high-density breeding layer can be made in high light side, low density cultivation layer is in low light or not irradiation side, further, from high-density to low density, the thickness of each cultivation layer increases successively, that is, the light path of each cultivation layer is made to increase successively; Like this, through high-density layer through the low light level can by low-density layer efficiency utilization, improve overall light utilising efficiency and micro-algae output, high-density breeding layer and low density cultivate layer coupling cultivation simultaneously, more overall employing high-density culture reduces mixing energy consumption, and avoid overall for when high strength mixes to the shear property model of cell.
Or as shown in Figure 2 and Figure 3, by outside to the inside of bioreactor 20 of bioreactor 20, the thickness of at least two cultivation layers increase successively, wherein, in Fig. 2, the thickness of the second cultivation layer 22 refers to its diameter.Like this, when this microalgae culturing device adopting two sides all receives illumination, by outside to the inside of bioreactor 20 of bioreactor 20, at least two intensities of illumination of receiving of cultivation layer weaken successively, at this moment, the inoculum density in the first cultivation layer 21 and the second cultivation layer 22 can be made to reduce successively, and, in breeding process, in each cultivation layer, pass into gas, and ensure that the ventilation ratio of the first cultivation layer 21 and the second cultivation layer 22 reduces successively.
That is, the cultivation layer be nested for two, skin can adopt higher density to cultivate, inside can adopt comparatively low density to cultivate, high-density breeding layer through the low light level can all be absorbed by the low-density micro-algae in inside and carry out photosynthesis, decrease thermosteresis and fluorescence losses, entirety improves the utilising efficiency of high-intensity illumination, and then improves overall yield; Outer high density and high light intensity are to the demand of mixture strength comparatively greatly simultaneously, and inside is due to low density and the low light level, can adopt lower mixture strength, and more overall employing high-density breeding reduces ventilation energy consumption, avoids high strength and mixes the shear property model brought.
It should be noted that, the bioreactor of at least two-layer superimposed form shown in Fig. 1 also can as illustrated in Fig. 2,3, cultivate the thickness of layer by outside at least two, the inside of bioreactor of bioreactor to increase successively, in such as Fig. 1, the first cultivation layer 11, second cultivates the relation of thickness L11, L12, L13 of layer 12 and the 3rd cultivation layer 13 can be L11=L13 < L12.
Fig. 2, the bioreactor of at least two-layer form that is nested shown in Fig. 3, due to the annular cultivation layer that bioreactor outer field cultivation layer is closed on the direction perpendicular to this bioreactor bottom surface, the i.e. outer annular cultivation layer for being communicated with, the algae liquid of this cultivation layer can be made to mix by mixing in breeding process, therefore, in breeding process, no matter be adopt the two sides of foregoing description all to receive illumination or adopt one side to receive illumination, by outside to the inside of bioreactor of bioreactor, in each cultivation layer, inoculum density can reduce successively, namely outer cultivation layer can adopt higher density and the cultivation of larger ventilation ratio, and inside can reduce cultivation density and ventilation ratio successively.
The breather arranged in each cultivation layer, for passing into proper amount of gas in breeding process in algae liquid, the gas mixture of such as carbonic acid gas and air, to make algae liquid fully mix, ensures the mixture strength needed for cultivation; Or can also whipping appts be provided with, while ventilation, algae liquid be stirred, ensure algae liquid Homogeneous phase mixing further, thus make micro-algae of cultivation all can receive appropriate illumination.Particularly, for the concrete structure of breather and whipping appts, those skilled in the art specifically can select according to physical conditions such as cultivation scales, and the present invention does not describe in detail this.
The bottom of microalgae culturing device adopting can also be provided with swivel arrangement, like this, in breeding process when one side receives high-intensity illumination, sunlight is such as used to make light source, when adopting microalgae culturing device adopting shown in Fig. 1, the first side 14 of microalgae culturing device adopting can also be made all the time towards light source by swivel arrangement (not shown), and the second side 15 is all the time away from light source.
The microalgae culturing device adopting that the embodiment of the present invention provides, the microalgae culturing device adopting of high light intensity, high-density, wide light path is improved, replace with and adopt high-density to be coupled with low density the mode cultivated, after improving cultivation strategy, achieve the coupling cultivation of multiple cultivation density, multiple mixture strength, improve the utilising efficiency of micro-algae to high light, and then improve output, reduce overall mixing energy consumption simultaneously, avoid high strength to mix the shearing action brought.
Correspondingly, as shown in Figure 4, the embodiment of the present invention additionally provides a kind of both culturing microalgae method, employs any one microalgae culturing device adopting that the embodiment of the present invention provides, and described both culturing microalgae method comprises the following steps:
S1, the intensity of illumination received according to each cultivation layer of microalgae culturing device adopting, algae kind be seeded in each cultivation layer, wherein, the inoculum density of each cultivation layer cultivates the reduction of the intensity of illumination that layer receives with each and reduces;
In S2, breeding process, in each cultivation layer, pass into gas, wherein, the ventilation ratio of each cultivation layer reduces with the reduction of the inoculum density of each cultivation layer.
The both culturing microalgae method that the embodiment of the present invention provides, the microalgae culturing device adopting adopted comprises bioreactor, and this bioreactor comprises at least two cultivation layers, during both culturing microalgae, according to the intensity of illumination that each cultivation layer of this microalgae culturing device adopting receives, in each cultivation layer, inoculate the algae kind of different densities, and make the inoculum density in each cultivation layer cultivate the reduction of the intensity of illumination that layer receives with each and reduce; In breeding process, pass into gas respectively in each cultivation layer, the ventilation ratio of each cultivation layer is reduced with the reduction of the inoculum density of each cultivation layer; Like this, high-intensity illumination irradiates the cultivation layer of high cultivation density, and can be absorbed by the micro-algae in the cultivation layer of low cultivation density thus carry out photosynthesis through the illumination that the intensity of high cultivation density cultivation layer is more weak; Like this, the both culturing microalgae method entirety that the embodiment of the present invention provides improves the utilization ratio of high-intensity illumination, and then improves overall yield; And, for the cultivation layer that the intensity of illumination received is higher, cultivation density wherein and ventilation ratio are all higher, and for the lower cultivation layer of the intensity of illumination received, cultivation density wherein and ventilation ratio are all lower, compared to the mode adopting high-density breeding overall in prior art, reduce ventilation energy consumption, avoid high strength and mix the shear property model brought.
In breeding process, microalgae culturing device adopting can receive illumination from one side, also all can receive illumination in two sides.
When the two sides of microalgae culturing device adopting all receives illumination, described both culturing microalgae method specifically can comprise:
S11, outside to the inside of bioreactor by bioreactor, the intensity of illumination that at least two cultivation layers receive weakens successively; Algae kind be seeded in each cultivation layer, wherein, by outside to the inside of bioreactor of bioreactor, the inoculum densities of at least two cultivation layers reduce successively;
In S12, breeding process, in each cultivation layer, pass into gas, wherein, by outside to the inside of bioreactor of bioreactor, the ventilation ratios of at least two cultivation layers reduce successively.
That is, when the two sides of microalgae culturing device adopting all receives illumination, the skin of bioreactor can adopt higher density to cultivate, inside can adopt comparatively low density to cultivate, high-density breeding layer through the low light level can all be absorbed by the low-density micro-algae in inside and carry out photosynthesis, decrease thermosteresis and fluorescence losses, entirety improves the utilising efficiency of high-intensity illumination, and then raising overall yield, simultaneously outer high density and the demand of high light intensity to mixture strength larger, and inside is due to low density and the low light level, lower mixture strength can be adopted, more overall employing high-density breeding reduces ventilation energy consumption, avoid high strength and mix the shear property model brought.
Further, when the two sides of microalgae culturing device adopting all receives illumination, before above-mentioned steps S11, described method can also comprise:
The thickness arranging each cultivation layer of bioreactor is all equal; That is, the thickness of each cultivation layer of the microalgae culturing device adopting now adopted can be identical, or,
Preferably, by outside to the inside of bioreactor of bioreactor, the thickness arranging at least two cultivation layers increase successively, and particularly, the microalgae culturing device adopting of employing such as can be as shown in Figure 2,3.Like this, according to intensity of illumination, adopt high-density, high mixture strength, short light path in the stronger side of intensity of illumination, and adopt low density, low mixture strength, long light path in the side that intensity of illumination is more weak, thus be conducive to making full use of and the raising of micro-algae output of sunlight.
When microalgae culturing device adopting receives illumination from one side, when bioreactor comprises at least two superimposed cultivation layers, described both culturing microalgae method specifically can comprise:
S21-1, the first side to second side relative with this first side by bioreactor, the intensity of illumination that at least two cultivation layers receive weakens successively; Algae kind being seeded to described in each cultivates in layer, wherein, second side extremely relative with this first side by the first side of bioreactor, the inoculum densities of at least two cultivation layers reduce successively;
In S22-1, breeding process, in each cultivation layer, pass into gas, wherein, second side extremely relative with this first side by the first side of bioreactor, the ventilation ratios of at least two cultivation layers reduce successively; Or,
When bioreactor comprise at least two be nested cultivation layer time, described both culturing microalgae method specifically can comprise:
S21-2, outside to the inside of bioreactor by bioreactor, the intensity of illumination that at least two cultivation layers receive weakens successively; Algae kind be seeded in each cultivation layer, wherein, by outside to the inside of bioreactor of bioreactor, the inoculum densities of at least two cultivation layers reduce successively;
In S22-2, breeding process, in each cultivation layer, pass into gas, wherein, by outside to the inside of bioreactor of bioreactor, the ventilation ratios of at least two cultivation layers reduce successively.
That is, when microalgae culturing device adopting receives illumination from one side, high-density breeding layer can be made in high light side, low density cultivation layer is in low light or not irradiation side; Like this, through high-density layer through the low light level can be utilized efficiently by low-density layer, improve overall light utilising efficiency and micro-algae output, high-density breeding layer and low density cultivate layer coupling cultivation simultaneously, more overall employing high-density culture reduces mixing energy consumption, and avoid overall for when high strength mixes to the shear property model of cell.
It should be noted that, Fig. 2, the bioreactor of at least two-layer form that is nested shown in Fig. 3, due to the annular cultivation layer that bioreactor outer field cultivation layer is closed on the direction perpendicular to this bioreactor bottom surface, namely the annular cultivation layer be communicated with, no matter be that one side receives illumination or two sides all receives illumination, this cultivation layer is in outermost layer all the time, stronger illumination can both be received, and the algae liquid of this layer can be made to mix by mixing in breeding process, therefore, in breeding process, no matter be adopt the two sides of foregoing description all to receive illumination or adopt one side to receive illumination, by outside to the inside of bioreactor of bioreactor, in each cultivation layer, inoculum density all can reduce successively, namely outer cultivation layer can adopt higher density and the cultivation of larger ventilation ratio, and inside can reduce cultivation density and ventilation ratio successively.
Further, when microalgae culturing device adopting receives illumination from one side, before above-mentioned steps S21-1, described method can also comprise:
The thickness arranging each cultivation layer of bioreactor is all equal, that is, the thickness of each cultivation layer of the microalgae culturing device adopting now adopted can be identical, or,
Preferably, second side extremely relative with the first side by the first side of bioreactor, the thickness arranging at least two cultivation layers increase successively, and this optical-biological reaction implement body such as can be as shown in Figure 1.Like this, according to intensity of illumination, adopt high-density, high mixture strength, short light path in the stronger side of intensity of illumination, and adopt low density, low mixture strength, long light path in the side that intensity of illumination is more weak, thus be conducive to making full use of and the raising of micro-algae output of sunlight.
Similarly, when microalgae culturing device adopting receives illumination from one side, before above-mentioned steps S21-2, described method can also comprise:
The thickness arranging each cultivation layer of bioreactor is all equal, that is, the thickness of each cultivation layer of the microalgae culturing device adopting now adopted can be identical, or,
By outside to the inside of bioreactor of bioreactor, the thickness arranging at least two cultivation layers increase successively, and this optical-biological reaction implement body such as can be as shown in Figure 2,3.
It should be noted that, under one side reception illumination occurs in outdoor cultivating condition mostly, in this case, microalgae culturing device adopting can be made to face east-west direction place, also it can be made to face North and South direction place: when microalgae culturing device adopting faces east-west direction placement, make high-density breeding layer (in such as Fig. 1 the first cultivation layer 11) be placed on the east side of low density cultivation layer (in such as Fig. 1 the 3rd cultivation layer 13) morning, then should be transferred to along with the change of light intensity the west side that low density cultivates layer in the afternoon; When microalgae culturing device adopting faces North and South direction placement, based on azimuths of the sun reason, sunlight whole day is radiated on the high-density breeding layer of southern side, low density cultivation layer is then positioned at the north side of high-density breeding layer, receive the low light level, therefore can not need to carry out change in location, still can complete high-density breeding layer and receive high light intensity, low density cultivation layer receives the culture condition of the low light level.
Or, in breeding process, when microalgae culturing device adopting from one side receive illumination and bioreactor comprises at least two superimposed cultivation layers time, above-mentioned steps S22 can also comprise: adjustment bioreactor sensitive surface towards, the intensity of illumination that first side joint of bioreactor is received is greater than the intensity of illumination that the second side joint receives always, namely make the high-density breeding layer of microalgae culturing device adopting towards light source all the time, make low density cultivate layer away from light source.Particularly, this process can by arranging swivel arrangement to realize in the bottom of microalgae culturing device adopting.
In breeding process, the illumination of employing can comprise artificial lighting or natural lighting, and intensity of illumination can be more than or equal to 500 μm of ol/m
2/ s, is preferably greater than or equal to 1000 μm of ol/m
2/ s, is preferably greater than or equal to 2000 μm of ol/m further
2/ s.Like this, the illumination of high strength can ensure the high yield of micro-algae.
The gas passed in each cultivation layer can be the gas mixture of carbonic acid gas and air, and the volume percent that carbonic acid gas accounts for gas mixture is 0.01 ~ 15%, the scope of ventilation ratio can be 0.1 ~ 10vvm, preferably 1 ~ 5vvm, to ensure fully to mix in each cultivation layer, improve output.
Further, before above-mentioned steps S1, described both culturing microalgae method can also comprise preparation work, such as can also comprise the steps:
S0, preparation substratum, remove supernatant liquor by rare algae seedling solution, obtain the algae seedling solution concentrated, think that subsequent inoculations reaches corresponding cell density and prepares.
In addition, in breeding process, step S2 can also comprise:
Control in the Suitable ranges of microalgae cell growth by the temperature of each cultivation layer, to ensure the normal growth of micro-algae, this optimal temperature is generally 15 ~ 35 DEG C.
In breeding process, step S2 can also comprise:
When the gas passed in each cultivation layer is the gas mixture of carbonic acid gas and air, by controlling the concentration of carbonic acid gas in gas mixture, control in microalgae cell optimum range by the pH of nutrient solution in each cultivation layer, the pH of this optimum range is generally 5 ~ 9, is preferably 6 ~ 8.
In addition, in order to make the growth conditions of microalgae cell preferably, in breeding process, step S2 can also comprise: the growing state detecting microalgae cell, carries out nutritional supplementation and adjustment growth conditions in time.Such as: by detecting absorbancy (OD value) or the dry cell weight of microalgae cell, when OD value or dry cell weight increment are less than first threshold, Additional nutrient solution, and/or, the pH value of adjustment nutrient solution or temperature.Particularly, this first threshold can change with the change of the cultivated actual parameter such as microalgae cell kind, culture condition, and those skilled in the art specifically can determine according to practical situation, and the present invention is not described in detail this.
Further, after step S2, described both culturing microalgae method can also comprise:
Gather: after cultivating some cycles, when reaching required biomass, or, when growth velocity is lower than Second Threshold, terminates to cultivate, gather.
Particularly, described some cycles and Second Threshold can change with the change of the cultivated actual parameter such as microalgae cell kind, culture condition, and those skilled in the art specifically can determine according to practical situation, and the present invention is not described in detail this.
The microalgae culturing device adopting that the embodiment of the present invention provides and method, adopt multilayer both culturing microalgae system, realize the cultivation strategy that microdisk electrode density classes is many, mixing demand type is many, improve the utilising efficiency of micro-algae to light, improve cultured output, reduce high-density, wide light path, mixing needed for high light intensity simultaneously and stir energy consumption.Specifically, for two-layer nested type microalgae culturing device adopting, skin can adopt higher density to cultivate, inside can reduce cultivation density, external mix strength demand is large, and inside can adopt the mixing that intensity is low, comparatively under this floor space, overall employing higher density is cultivated and is reduced ventilation energy consumption, simultaneously higher density cultivation layer through the low light level effectively can be absorbed by the low-density micro-algae in inside, while not damaging low density activity of microalgae, improve the utilising efficiency of high light, and then improve overall yield.
The protection point of this patent is multilayer both culturing microalgae strategy and system.
The microalgae culturing device adopting provided the embodiment of the present invention below by specific embodiment and method are further elaborated.
First group of experiment
Comparative example 1
Microalgae culturing device adopting: employing diameter is 4cm, is highly the cylindrical organosilicon reaction device of H.
Inoculum density: preparation substratum, be seeded in reactor after concentrated algae kind, inoculum density is 30OD, and wherein, OD is absorbancy, linear with cell concn.
Aeration form: adopt annular aeration tube to carry out aeration, is provided with one group of annular aeration tube in reactor, adopt the logical mode adding the gas mixture of carbonic acid gas and air in reactor to carry out aeration in breeding process.
Ventilation ratio: the ventilation ratio in reactor is 3vvm, namely per minute needs are logical adds 12 liters of gas mixtures.
Light source: adopt source of artificial light to carry out double face lighting, the intensity of illumination often organizing light source is 500 μm of ol/m
2/ s, light vertical irradiation reactor, Light To Dark Ratio is 16:8, i.e. illumination 16h, dark 8h;
Adopt above condition to carry out microalgae cell cultivation, in breeding process, measure dry cell weight every 24h, calculate day increment, cultivate 5 days.
Embodiment 1
Microalgae culturing device adopting: employing height is as shown in Figure 2 the double-layer circular column type nested type organosilicon reaction device of H, comprise internal layer and outer two cultivation layers, wherein, internal layer light path (i.e. internal layer diameter) 3cm, outer light path (i.e. thickness) 1cm.
Inoculum density: preparation substratum, is seeded in reactor after concentrated algae kind, and reduce gradually from reactor skin to its inoculum density of internal layer, be respectively: outer field inoculum density is 60OD, the inoculum density of internal layer is 20OD.
Aeration form: adopt annular aeration tube to carry out aeration, the internal layer of reactor and skin are respectively equipped with one group of annular aeration tube, adopt the mode of leading to the gas mixture adding carbonic acid gas and air to the internal layer of reactor and skin to carry out aeration in breeding process respectively.
Ventilation ratio: from reactor skin to internal layer, its mixture strength reduces gradually: the outer field ventilation ratio of reactor is 3vvm, namely per minute needs are logical adds 3 liters of gas mixtures; The ventilation ratio of reactor internal layer is 1vvm, and namely per minute needs are logical adds 3 liters of gas mixtures.
Light source: adopt source of artificial light to carry out double face lighting, the intensity of illumination often organizing light source is 500 μm of ol/m
2/ s, light vertical irradiation reactor, Light To Dark Ratio is 16:8, i.e. illumination 16h, dark 8h;
Adopt above condition to carry out microalgae cell cultivation, in breeding process, measure dry cell weight every 24h, calculate day increment, cultivate 5 days.
That is, in comparative example 1 and embodiment 1, their cultivation height and cultivation cumulative volume are identical, and for identical floor space, total OD number of their inoculum densities is identical; The cultivating conditions such as their aeration form, the light source that adopts also are identical.
Experimental result
5 days mean yields of embodiment 1 are about 4.8g/ days, and 5 days mean yields of comparative example 1 are about 3.6g/ days, and embodiment 1 is comparative example 1 output increased 33% comparatively, but the overall energy consumption of embodiment 1 is 50% of comparative example 1.
Calculation Method of Energy Consumption: comparative example 1 energy consumption equals the volume corresponding to 3vvm × 4cm light path, the embodiment 1 energy consumption volume equaled corresponding to 1vvm × 3cm light path adds the volume corresponding to 3vvm × 1cm light path, due to cultivation highly identical, the volume corresponding to different light path and light path proportional.
Energy consumption comparative result is: the energy consumption 12 of comparative example 1 is doubly to cultivation height H, and the energy consumption 6 of embodiment 1 is doubly to cultivation height H.Therefore, the overall energy consumption of embodiment 1 is 50% of comparative example 1.
Or the difference of the gas volume that also can be passed into by the unit time is adjusted, comparative example 1 per minute ventilates 12 liters, and embodiment 1 per minute ventilates 6 liters, and embodiment 1 is 50% of comparative example 1 energy consumption.
Result shows: outer 1cm light path high-density culture through the low light level by the microalgae cell efficient absorption of internal layer 3cm light path low density cell culture, achieve the efficiency utilization of high light, comparatively 4cm light path high-density culture, decreases total cellular score, reduce cellular respiration consumption, significantly promoting has appearred in output; 4cm light path high-density culture is adjusted to 1cm light path high-density culture and 3cm light path low density cell culture, reducing overall is mixing energy consumption during 4cm light path high-density culture simultaneously.
Second group of experiment
Comparative example 2
Microalgae culturing device adopting: employing length is L, is highly board-like organosilicon reaction device that H, thickness are 6cm.
Inoculum density: preparation substratum, be seeded in reactor after concentrated algae kind, inoculum density is 20OD, and wherein, OD is absorbancy, linear with cell concn.
Aeration form: adopt annular aeration tube to carry out aeration, is provided with one group of annular aeration tube in reactor, adopt the logical mode adding the gas mixture of carbonic acid gas and air in reactor to carry out aeration in breeding process.
Ventilation ratio: the ventilation ratio in reactor is 4vvm, namely per minute needs are logical adds 24 liters of gas mixtures.
Light source: adopt source of artificial light to carry out double face lighting, the intensity of illumination often organizing light source is 600 μm of ol/m
2/ s, light vertical irradiation reactor, Light To Dark Ratio is 16:8, i.e. illumination 16h, dark 8h;
Adopt above condition to carry out microalgae cell cultivation, in breeding process, measure dry cell weight every 24h, calculate day increment, cultivate 5 days.
Embodiment 2
Microalgae culturing device adopting: adopt length to be as shown in Figure 3 L, highly to be the Double-layer plate type nested type organosilicon reaction device of H, comprise internal layer and outer two cultivation layers, wherein, internal layer light path (i.e. internal layer thickness) 4cm, outer light path (i.e. thickness) 2cm.
Inoculum density: preparation substratum, is seeded in reactor after concentrated algae kind, and reduce gradually from reactor skin to its inoculum density of internal layer, be respectively: outer field inoculum density is 30OD, the inoculum density of internal layer is 15OD.
Aeration form: adopt annular aeration tube to carry out aeration, the internal layer of reactor and skin are respectively equipped with one group of annular aeration tube, adopt the mode of leading to the gas mixture adding carbonic acid gas and air to the internal layer of reactor and skin to carry out aeration in breeding process respectively.
Ventilation ratio: from reactor skin to internal layer, its mixture strength reduces gradually: the outer field ventilation ratio of reactor is 2.5vvm, namely per minute needs are logical adds 5 liters of gas mixtures; The ventilation ratio of reactor internal layer is 0.5vvm, and namely per minute needs are logical adds 2 liters of gas mixtures.
Light source: adopt source of artificial light to carry out double face lighting, the intensity of illumination often organizing light source is 600 μm of ol/m
2/ s, light vertical irradiation reactor, Light To Dark Ratio is 16:8, i.e. illumination 16h, dark 8h;
Adopt above condition to carry out microalgae cell cultivation, in breeding process, measure dry cell weight every 24h, calculate day increment, cultivate 5 days.
That is, in comparative example 2 and embodiment 2, their cultivation height and cultivation cumulative volume are identical, and for identical floor space, total OD number of their inoculum densities is identical; The cultivating conditions such as their aeration form, the light source that adopts also are identical.
Embodiment 3
Adopt mode substantially the same manner as Example 2 to carry out both culturing microalgae, difference part is only: the intensity of illumination often organizing light source in embodiment 3 is 1000 μm of ol/m
2/ s.
Experimental result
5 days mean yields of embodiment 2 are about 7.5g/ days, and 5 days mean yields of embodiment 3 are about 8.4g/ days, and 5 days mean yields of comparative example 2 are about 6.0g/ days; Embodiment 2 is comparative example 2 output increased 25% comparatively, embodiment 3 is comparative example 2 output increased 40% comparatively, and meanwhile, embodiment 3 is embodiment 2 output increased 12% comparatively; But the overall energy consumption of embodiment 2, embodiment 3 is 29.2% of comparative example 2.Calculation Method of Energy Consumption is identical with above-mentioned first group of Calculation Method of Energy Consumption of testing, and at this, it is no longer repeated.
This experimental result is consistent with the experimental result that first group is tested, show: 6cm light path high-density culture is adjusted to the form that 2cm light path high-density culture and 4cm light path low density cell culture are coupled, outer high-density through the low light level absorbed by the microalgae cell of internal layer low density cell culture, achieve the efficiency utilization of high light, comparatively 6cm light path high-density culture, decrease total cellular score, and then reduce respiration consumption, improve output; Reduce the mixing energy consumption of entirety when being 6cm light path high-density culture, when avoiding wide light path high-density culture, high strength mixes the shearing action brought, and has certain promotion to output increased simultaneously.
3rd group of experiment
Comparative example 3
Experimental Background: outside scenery, reactor faces thing and places, and namely sensitive surface is towards east-west direction, and receive high light, the west low light level in the east in the morning, and afternoon, west received high light, in the east the low light level.
Microalgae culturing device adopting: employing length is L, is highly board-like organosilicon reaction device that H, thickness are 6cm.
Inoculum density: preparation substratum, be seeded in reactor after concentrated algae kind, inoculum density is 20OD, and wherein, OD is absorbancy, linear with cell concn.
Aeration form: adopt annular aeration tube to carry out aeration, is provided with one group of annular aeration tube in reactor, adopt the logical mode adding the gas mixture of carbonic acid gas and air in reactor to carry out aeration in breeding process.
Ventilation ratio: the ventilation ratio in reactor is 3vvm, namely per minute needs are logical adds 18 liters of gas mixtures.
Adopt above condition to carry out microalgae cell cultivation, in breeding process, measure dry cell weight every 24h, calculate day increment, cultivate 5 days.
Embodiment 4
Experimental Background: outside scenery, reactor faces thing and places, and namely sensitive surface is towards east-west direction, and receive high light, the west low light level in the east in the morning, and afternoon, west received high light, in the east the low light level.
Microalgae culturing device adopting: adopt length to be as shown in Figure 1 L, highly to be the three ply board formula superposing type organosilicon reaction device of H, every layer of light path (or thickness) is respectively: 1cm, 2cm, 3cm.
Inoculum density: preparation substratum, be seeded in reactor after concentrated algae kind, reduce gradually to its inoculum density of the second side from reactor first side, be respectively: high light layer 1cm light path cultivation layer 40OD, middle photosphere 2cm light path cultivation layer 28OD, dysphotic zone 3cm light path cultivation layer 8OD.
Aeration form: adopt annular aeration tube to carry out aeration, is equipped with one group of annular aeration tube in every layer of reactor, adopt the mode that each cultivation layer to reactor leads to the gas mixture adding carbonic acid gas and air to carry out aeration in breeding process respectively.
Ventilation ratio: reduce gradually to its mixture strength of the second side from reactor first side: 1cm light path cultivation layer 3vvm, namely per minute ventilates 3 liters, 2cm light path cultivation layer 2vvm, and namely per minute ventilates 4 liters, and 3cm light path cultivation layer 1vvm, namely per minute ventilates 3 liters.
Light source: adopt nature sunlight to make light source, in breeding process, upper period of the day from 11 a.m. to 1 p.m, the first side of reactor was placed in the east, and the lower period of the day from 11 a.m. to 1 p.m, along with the change of irradiation direction, will be transferred to west and places.
Adopt above condition to carry out microalgae cell cultivation, in breeding process, measure dry cell weight every 24h, calculate day increment, cultivate 5 days.
That is, in comparative example 3 and embodiment 4, their cultivation height and cultivation cumulative volume are identical, and for identical floor space, total OD number of their inoculum densities is identical; The cultivating conditions such as their aeration form, the light source that adopts also are identical.
Experimental result
5 days mean yields of embodiment 4 are about 6.0g/ days, and 5 days mean yields of comparative example 3 are about 4.8g/ days; Embodiment 4 is comparative example 3 output increased 25% comparatively, but the overall energy consumption of embodiment 4 is 55.6% of comparative example 3.Calculation Method of Energy Consumption is identical with above-mentioned first group of Calculation Method of Energy Consumption of testing, and at this, it is no longer repeated.
This experimental result shows: while reducing high-density optical path width, increase cell density, coupling low density is cultivated, all high-density culture is adopted relative to identical floor space, decrease high-density culturing cell sum, reduce night respiration effect to the consumption of biomass, simultaneously through the low light level of high-density culture layer by low-density layer efficiency utilization, improve light utilising efficiency, and then improve output.
In addition, by the 6cm light path reactor of identical floor space, replace with the reactor coupling cultivation that 1cm, 2cm and 3cm light path is superimposed, reduce the mixing energy consumption of culturing process, when avoiding wide light path high-density culture, high strength mixes the shearing action brought, and has certain promotion to output increased.
The above; be only the specific embodiment of the present invention, but protection scope of the present invention is not limited thereto, is anyly familiar with those skilled in the art in the technical scope that the present invention discloses; change can be expected easily or replace, all should be encompassed within protection scope of the present invention.Therefore, protection scope of the present invention should be as the criterion with the protection domain of described claim.
Claims (10)
1. a microalgae culturing device adopting, is characterized in that, comprising: bioreactor, and described bioreactor comprises at least two cultivation layers, is equipped with breather, or is equipped with breather and whipping appts in each described cultivation layer.
2. microalgae culturing device adopting according to claim 1, is characterized in that,
Described bioreactor comprises at least two superimposed cultivation layers; Or,
Described bioreactor comprises at least two cultivation layers be nested.
3. microalgae culturing device adopting according to claim 1, is characterized in that,
The thickness cultivating layer described in each is equal; Or
Second side extremely relative with described first side by the first side of described bioreactor, the thickness of at least two described cultivation layers increases successively; Or
By outside to the inside of described bioreactor of described bioreactor, the thickness of at least two described cultivation layers increases successively.
4. microalgae culturing device adopting according to claim 1, is characterized in that, the bottom of described bioreactor is provided with swivel arrangement.
5. a both culturing microalgae method, is characterized in that, employs the microalgae culturing device adopting according to any one of Claims 1 to 4, and described both culturing microalgae method comprises step:
1) according to the intensity of illumination that each cultivation layer of described microalgae culturing device adopting receives, algae kind be seeded to described in each and cultivate in layer, wherein, the inoculum density cultivating layer described in each reduces with the reduction cultivating the intensity of illumination that layer receives described in each;
2), in breeding process, pass into gas to cultivating in layer described in each, wherein, the ventilation ratio cultivating layer described in each with cultivate described in each layer inoculum density reduction and reduce.
6. both culturing microalgae method according to claim 5, is characterized in that,
When the two sides of described microalgae culturing device adopting all receive illumination and/or when described bioreactor comprise at least two be nested cultivation layer time, described both culturing microalgae method specifically comprises:
1) by outside to the inside of described bioreactor of bioreactor, the intensity of illumination that at least two described cultivation layers receive weakens successively; Algae kind being seeded to described in each cultivates in layer, and wherein, by outside to the inside of described bioreactor of described bioreactor, the inoculum density of at least two described cultivation layers reduces successively;
2) in breeding process, pass into gas to cultivating in layer described in each, wherein, by outside to the inside of described bioreactor of described bioreactor, the ventilation ratio of at least two described cultivation layers reduces successively;
When described microalgae culturing device adopting from one side receive illumination and described bioreactor comprises at least two superimposed cultivation layers time, described both culturing microalgae method specifically comprises:
1) by the first side to second side relative with described first side of bioreactor, the intensity of illumination that at least two described cultivation layers receive weakens successively; Algae kind being seeded to described in each cultivates in layer, wherein, second side extremely relative with described first side by the first side of described bioreactor, the inoculum density of at least two described cultivation layers reduces successively;
2) in breeding process, pass into gas to cultivating in layer described in each, wherein, second side extremely relative with described first side by the first side of described bioreactor, the ventilation ratio of at least two described cultivation layers reduces successively.
7. both culturing microalgae method according to claim 6, is characterized in that, step 1) before, described method also comprises:
Arrange described bioreactor each described in cultivate the thickness of layer all equal; Or,
When the two sides of described microalgae culturing device adopting all receive illumination and/or when described bioreactor comprise at least two be nested cultivation layer time, by outside to the inside of described bioreactor of described bioreactor, the thickness arranging at least two described cultivation layers increases successively;
When described microalgae culturing device adopting from one side receive illumination and described bioreactor comprises at least two superimposed cultivation layers time, second side extremely relative with described first side by the first side of described bioreactor, the thickness arranging at least two described cultivation layers increases successively.
8. both culturing microalgae method according to claim 6, it is characterized in that, in breeding process, when described microalgae culturing device adopting from one side receive illumination and described bioreactor comprises at least two superimposed cultivation layers time, step 2) also comprise: the sensitive surface adjusting described bioreactor towards, the intensity of illumination that the first side joint of described bioreactor is received is greater than the intensity of illumination that described second side joint receives always.
9. the both culturing microalgae method according to any one of claim 5 ~ 8, is characterized in that, described illumination comprises artificial lighting or natural lighting, and described intensity of illumination is more than or equal to 500 μm of ol/m
2/ s.
10. the both culturing microalgae method according to any one of claim 5 ~ 8, is characterized in that, described ventilation ratio is 0.1 ~ 10vvm.
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