CN104569232A - High-performance liquid chromatographic ball - Google Patents
High-performance liquid chromatographic ball Download PDFInfo
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- CN104569232A CN104569232A CN201410764872.5A CN201410764872A CN104569232A CN 104569232 A CN104569232 A CN 104569232A CN 201410764872 A CN201410764872 A CN 201410764872A CN 104569232 A CN104569232 A CN 104569232A
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Abstract
The invention relates to a high-performance liquid chromatographic ball, which comprises a pre-column, a ball body and a first flange device, wherein the first flange device is connected with the pre-column and the ball body; the ball body has a cavity structure composed of an upper half ball and a lower half ball which are connected through a second flange device, an upper pipe body communicated with the cavity is arranged at the top of the upper half ball, a pipe orifice of the upper pipe body is fixedly connected with the pre-column through the first flange device, and a lower pipe body communicated with the cavity is arranged on the bottom end of the lower half ball. The technical problems that the liquid chromatographic column is commonly instable, and easily damaged can be solved, and the liquid chromatographic ball has the advantages of being simple in structure, large in injection volume, and stable in the column body.
Description
Technical field
The present invention relates to liquid-phase chromatographic column technical field, be specifically related to a kind of high performance liquid chromatography ball.
Background technology
Liquid phase chromatography is an extremely important branch in red, orange, green, blue, yellow (ROGBY), it compensate for the limitation that gas chromatography is analyzed high boiling point organic compound, a point analysis of variance can be carried out to the organism of nature 80 percent, and gas chromatography only can carry out compartment analysis to the sample of whole organic compound 20 percent, the application of liquid phase chromatography makes chemico-analytic field more vast.
Liquid chromatography is separated to be remembered to be used to effectively be separated the blend sample with multiple component, and core component is liquid chromatography separation column.Its separation principle is, blending ingredients sample is dissolved in a kind of mobile phase, mobile phase is under larger pressure condition, the chromatograph packing material be seated in chromatographic column is flow through with certain flow velocity, because chromatograph packing material is to the difference of the absorption affinity of different component, different component flows through chromatograph packing material by with different flow velocitys, and in the detecting device of liquid chromatograph, detect that different component flows out the time of chromatographic column and the content of each component, thus the object reaching separation, detect.The liquid-phase chromatographic column of the present overwhelming majority still adopts traditional cylindrical, and traditional chromatographic column is the fractional dose increasing chromatographic column, and the method often by increasing column diameter increases the consumption of Stationary liquid, thus reaches the object of increase point strength.But along with the increase of column diameter, the Stationary liquid used in separation is in the reason of economy and equipment, use the parting material of larger particles, this makes in the filling process of chromatographic column, and easily occur directed classification phenomenon, the separating effect of chromatographic column is low.
Therefore, for the cylinder solving the existence of above-mentioned existing chromatographic column is unstable, applied sample amount is little and easily by the defect of high mobile phase linear speed destruction, overall performance difference, applicant develops the chromatogram ball of novel structure.
Summary of the invention
The technical problem to be solved in the present invention is to provide a kind of high performance liquid chromatography ball, and which solve the technical matters of the ubiquitous cylinder instability of liquid-phase chromatographic column, destructible, this liquid chromatography ball has the advantage that structure is simple, applied sample amount is large, cylinder is stable.
For solving the problems of the technologies described above, the present invention includes following technical scheme:
A kind of high performance liquid chromatography ball, chromatogram bag draws together pre-column, spheroid and the first flange apparatus;
Spheroid is the cavity constructions that episphere and lower semisphere are connected to form by the second flange apparatus, the top of episphere is provided with the upper tube body being communicated with cavity, the mouth of pipe of upper tube body is fixedly connected with by described first flange apparatus with between pre-column, and the bottom of described lower semisphere is provided with the lower tube body being communicated with cavity.
Further, pre-column is hollow cavity structure, and the cylinder first half of described pre-column be column cap, Lower Half is shower nozzle, is provided with filtration unit in the cavity of column cap and shower nozzle junction; The cavity xsect of column cap from top to bottom increases gradually, and this structure can make the sample dispense of separation more even, effectively strengthens applied sample amount.
Pre-column of the present invention is the destruction preventing high mobile phase linear speed coupled columns bed, the cavity xsect of shower nozzle is designed to the structure reduced gradually from top to bottom.
For making whole pre-column while meeting above-mentioned effect, its appearance design is more rationalized, global design column cap and shower nozzle are connected to form further becomes the spheroid form of band cavity; The upper end of column cap is provided with pipeline, and the lower end of shower nozzle is provided with the lower pipeline being connected to described first flange apparatus; The first sieve plate is provided with in the pipe of lower pipeline.
Further, between the inwall of the first flange apparatus and lower pipeline, between the first flange apparatus and the inwall of upper tube body, the first O-ring seal is respectively equipped with.
Pre-column of the present invention can be started discharging and refill, and namely column cap and shower nozzle are for removably connecting, and removably connect and are specially: the link of column cap and shower nozzle is threaded, and both are connected by threaded engagement; The second O-ring seal is also provided with in described threaded engagement junction.
Further, the upper end of the second flange apparatus is provided with filter.
Extend in the internal chamber wall of the lower semisphere of chromatogram ball of the present invention is outer gradually, easily cause wall effect; For solving this problem, make sample separation collect process in the layering of lower semisphere more smooth and easy, the position upwards extending 1/3 in the bottom of lower semisphere is provided with a base, and the upper orifice of lower tube body extends upwardly to base upper end, and diameter increases formation hydraucone gradually.
Further, the top of base is provided with cylinder manifold, and the top of cylinder manifold is provided with the second sieve plate.
Further, be provided with surrounding layer outside spheroid, the material of surrounding layer is epoxy resin material.
After adopting technique scheme, the present invention at least has the following advantages:
1) chromatogram ball of the present invention is compared with traditional chromatographic column, and modern design, structure are simple and cylinder is stable.
2) the present invention realizes the separation of better Quality control by the spheroid of bulbous configuration: when sample separation is down oozed in episphere cavity, because cavity cross section area increases gradually, make solute molecule be not easy to reach tube wall district in downflow processes, thus improve post effect; In addition, episphere is connected by ring flange with lower semisphere, further avoid wall flow phenomenon, achieves the combination of chromatographic resolution and enrichment process, is conducive to collection and the control of sample.
3) pre-column applied sample amount of the present invention is large, adopts cavity xsect to be designed to reduce design from top to bottom gradually, make sample dispense more even like this by the column cap of pre-column.
4) design of the cavity xsect of pre-column shower nozzle, can prevent the destruction of high mobile phase linear speed coupled columns bed.
Accompanying drawing explanation
Above-mentioned is only the general introduction of technical solution of the present invention, and in order to better understand technological means of the present invention, below in conjunction with accompanying drawing and embodiment, the present invention is described in further detail.
Fig. 1 is the structural representation of the embodiment of the present invention 1;
Fig. 2 is the structural representation of the embodiment of the present invention 2;
Fig. 3 is the cut-open view of the embodiment of the present invention 2;
Fig. 4 is the partial enlarged drawing of the position I of Fig. 3;
Fig. 5 is the partial enlarged drawing that the first flange apparatus of the embodiment of the present invention 2 connects;
Fig. 6 is the cut-open view of the embodiment of the present invention 3.
Embodiment
embodiment 1
A kind of high performance liquid chromatography ball, shown in Fig. 1, chromatogram bag is drawn together pre-column 1, spheroid 2 and is connected the first flange apparatus 3 of pre-column 1 and spheroid 2.
Spheroid 2 is the cavity constructions that episphere 7 and lower semisphere 8 are connected to form by the second flange apparatus 9, the top of episphere 7 is provided with the upper tube body 70 being communicated with cavity, the mouth of pipe of upper tube body 70 is fixedly connected with by the first flange apparatus 3 with between pre-column 1, and the bottom of lower semisphere 8 is provided with the lower tube body 80 being communicated with cavity.
embodiment 2
The present embodiment is the another kind of implementation method of high performance liquid chromatography ball of the present invention, as different from Example 1, shown in Fig. 2 ~ 3, pre-column 1 is hollow cavity structure, and the cylinder first half of pre-column 1 be column cap 4, Lower Half is shower nozzle 5, is provided with filtration unit 6 in the cavity of column cap 4 and shower nozzle 5 junction; The cavity xsect of column cap 4 from top to bottom increases gradually, and the cavity xsect of shower nozzle 5 from top to bottom reduces gradually.
Pre-column of the present invention, in the prerequisite meeting above-mentioned structure, does not do concrete restriction to it, and the present embodiment is preferred, the spheroid form being connected to form band cavity of column cap 4 and shower nozzle 5; The upper end of column cap 4 is provided with pipeline 40, and the lower end of shower nozzle 5 is provided with the lower pipeline 50 being connected to described first flange apparatus 3; The first sieve plate 11 is provided with in the pipe of lower pipeline 50.
Shown in Fig. 4, Fig. 5, between the inwall of the first flange apparatus 3 and lower pipeline 50, between the first flange apparatus 3 and the inwall of upper tube body 70, be respectively equipped with the first O-ring seal 30.
Column cap 4 and shower nozzle 5 are for removably connecting, and the present embodiment preferably mode of removably connecting is: the link of column cap 4 and shower nozzle 5 is threaded, and both are connected by threaded engagement; The second O-ring seal 90 is also provided with in described threaded engagement junction.
embodiment 3
The present embodiment is another implementation method of high performance liquid chromatography ball of the present invention, and as different from Example 1, the bottom of lower semisphere 8 is provided with confluence mechanism, description specific as follows.
Shown in Fig. 6, the position that the bottom of lower semisphere 8 upwards extends 1/3 is provided with base 12, and the upper orifice of lower tube body 8 extends upwardly to base 12 upper end, and diameter increases gradually and forms hydraucone 81.The top of base 12 is provided with cylinder manifold 13, and the top of cylinder manifold 13 is provided with the second sieve plate 14.The upper end of the second flange apparatus 9 is provided with filter 15.
Chromatogram spherical structure of the present invention is simple, and its spheroid 2 is outer also can be provided with surrounding layer 10, and the material of surrounding layer 10 is epoxy resin material.
The filler of chromatogram ball is filled in pre-column and spheroid, and during use, sample separation is poured into from Shangguan road 40 of column cap 4, after filtrator 6, enter spheroid 2 by shower nozzle 5, and sample flow is separated, and finally collects from the lower end of the lower tube body 80 of lower semisphere 8.
The above embodiment is only that the preferred embodiment of the present invention is described; not scope of the present invention is limited; under not departing from the present invention and designing the prerequisite of spirit; the various distortion that those of ordinary skill in the art make technical scheme of the present invention and improvement, all should fall in protection domain that claims of the present invention determine.
Claims (10)
1. a high performance liquid chromatography ball, is characterized in that, described chromatogram bag draws together pre-column (1), spheroid (2) and the first flange apparatus (3);
The cavity constructions that described spheroid (2) is connected to form by the second flange apparatus (9) for episphere (7) and lower semisphere (8), the top of described episphere (7) is provided with the upper tube body (70) being communicated with cavity, the mouth of pipe of described upper tube body (70) is fixedly connected with by described first flange apparatus (3) with between pre-column (1), and the bottom of described lower semisphere (8) is provided with the lower tube body (80) being communicated with cavity.
2. high performance liquid chromatography ball as claimed in claim 1, it is characterized in that, described pre-column (1) is hollow cavity structure, and the cylinder first half of described pre-column (1) be column cap (4), Lower Half is shower nozzle (5), is provided with filtration unit (6) in the cavity of described column cap (4) and shower nozzle (5) junction; The cavity xsect of described column cap (4) from top to bottom increases gradually.
3. high performance liquid chromatography ball as claimed in claim 2, it is characterized in that, the cavity xsect of described shower nozzle (5) from top to bottom reduces gradually.
4. high performance liquid chromatography ball as claimed in claim 3, is characterized in that, described column cap (4) and shower nozzle (5) are connected to form the spheroid form of band cavity; The upper end of described column cap (4) is provided with pipeline (40), and the lower end of described shower nozzle (5) is provided with the lower pipeline (50) being connected to described first flange apparatus (3); The first sieve plate (11) is provided with in the pipe of described lower pipeline (50).
5. high performance liquid chromatography ball as claimed in claim 4, it is characterized in that, between described first flange apparatus (3) and the inwall of lower pipeline (50), between the first flange apparatus (3) and the inwall of upper tube body (70), be respectively equipped with the first O-ring seal (30).
6. high performance liquid chromatography ball as claimed in claim 4, it is characterized in that, described column cap (4) and shower nozzle (5) are for removably connecting, described removably connecting is specially: the link of column cap (4) and shower nozzle (5) is threaded, and both are connected by threaded engagement; The second O-ring seal (90) is also provided with in described threaded engagement junction.
7. high performance liquid chromatography ball as claimed in claim 1, it is characterized in that, the upper end of described second flange apparatus (9) is provided with filter (15).
8. high performance liquid chromatography ball as claimed in claim 1, it is characterized in that, the position that the bottom of described lower semisphere (8) upwards extends 1/3 is provided with base (12), the upper orifice of described lower tube body (8) extends upwardly to base (12) upper end, and diameter increases formation hydraucone (81) gradually.
9. high performance liquid chromatography ball as claimed in claim 8, it is characterized in that, the top of described base (12) is provided with cylinder manifold (13), and the top of described cylinder manifold (13) is provided with the second sieve plate (14).
10. high performance liquid chromatography ball as claimed in claim 1, it is characterized in that, be provided with surrounding layer (10) outside described spheroid (2), the material of described surrounding layer (10) is epoxy resin material.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410764872.5A CN104569232B (en) | 2014-12-11 | 2014-12-11 | High-performance liquid chromatographic ball |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410764872.5A CN104569232B (en) | 2014-12-11 | 2014-12-11 | High-performance liquid chromatographic ball |
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| Publication Number | Publication Date |
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| CN104569232A true CN104569232A (en) | 2015-04-29 |
| CN104569232B CN104569232B (en) | 2017-01-18 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN201410764872.5A Active CN104569232B (en) | 2014-12-11 | 2014-12-11 | High-performance liquid chromatographic ball |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108700561A (en) * | 2016-01-06 | 2018-10-23 | 通用电气医疗集团生物工艺研发股份公司 | Method and apparatus for packing chromatography column assembly |
Citations (6)
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| US3334514A (en) * | 1964-12-16 | 1967-08-08 | Technicon Corp | Chromatographic column |
| CN201522480U (en) * | 2009-09-23 | 2010-07-07 | 华东理工大学 | Spherical high-performance liquid chromatogram preparation column |
| US20100200488A1 (en) * | 2007-09-06 | 2010-08-12 | Francois Couillard | Cells and connecting channels for centrifugal partition chromatography devices |
| CN102621259A (en) * | 2011-03-24 | 2012-08-01 | 苏州汇通色谱分离纯化有限公司 | Capillary parabolic shape capillary liquid chromatography preparative column |
| CN204359761U (en) * | 2014-12-11 | 2015-05-27 | 首都师范大学 | high performance liquid chromatography ball |
| CN204359791U (en) * | 2014-12-26 | 2015-05-27 | 中南大学 | A kind of Immunofluorescence test paper strip |
-
2014
- 2014-12-11 CN CN201410764872.5A patent/CN104569232B/en active Active
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US3334514A (en) * | 1964-12-16 | 1967-08-08 | Technicon Corp | Chromatographic column |
| US20100200488A1 (en) * | 2007-09-06 | 2010-08-12 | Francois Couillard | Cells and connecting channels for centrifugal partition chromatography devices |
| CN201522480U (en) * | 2009-09-23 | 2010-07-07 | 华东理工大学 | Spherical high-performance liquid chromatogram preparation column |
| CN102621259A (en) * | 2011-03-24 | 2012-08-01 | 苏州汇通色谱分离纯化有限公司 | Capillary parabolic shape capillary liquid chromatography preparative column |
| CN204359761U (en) * | 2014-12-11 | 2015-05-27 | 首都师范大学 | high performance liquid chromatography ball |
| CN204359791U (en) * | 2014-12-26 | 2015-05-27 | 中南大学 | A kind of Immunofluorescence test paper strip |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108700561A (en) * | 2016-01-06 | 2018-10-23 | 通用电气医疗集团生物工艺研发股份公司 | Method and apparatus for packing chromatography column assembly |
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| CN104569232B (en) | 2017-01-18 |
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