CN104562213A - 扩增子文库及其构建方法 - Google Patents

扩增子文库及其构建方法 Download PDF

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CN104562213A
CN104562213A CN201410835879.1A CN201410835879A CN104562213A CN 104562213 A CN104562213 A CN 104562213A CN 201410835879 A CN201410835879 A CN 201410835879A CN 104562213 A CN104562213 A CN 104562213A
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pcr
construction process
sample
amplified production
joint
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Chinese (zh)
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曹志生
王大伟
杜长诗
朱海浩
刘运超
魏勤
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Nuo Hezhi Source Beijing Bioinformation Science And Technology Ltd
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Nuo Hezhi Source Beijing Bioinformation Science And Technology Ltd
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CN201410835879.1A 2014-12-26 2014-12-26 扩增子文库及其构建方法 Pending CN104562213A (zh)

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Cited By (34)

* Cited by examiner, † Cited by third party
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CN105779437A (zh) * 2016-03-11 2016-07-20 台州黄岩圣庭医学检验所 一种基于Ion PGMTM测序平台的扩增子DNA文库的构建方法、试剂及其应用
CN106282177A (zh) * 2016-08-23 2017-01-04 厦门基源医疗科技有限公司 一种16S rDNA高通量测序文库的构建方法
CN106811460A (zh) * 2015-11-30 2017-06-09 安诺优达基因科技(北京)有限公司 用于低频突变检测的二代测序文库的构建方法及试剂盒
CN106835291A (zh) * 2016-12-14 2017-06-13 广州燃石医学检验所有限公司 Dna 文库的制备方法以及试剂盒
CN107058573A (zh) * 2016-06-13 2017-08-18 艾吉泰康生物科技(北京)有限公司 一种利用Cas9/gRNA系统构建扩增子文库的方法
WO2017219511A1 (zh) * 2016-06-22 2017-12-28 杭州杰毅麦特医疗器械有限公司 一种快速均一化或等比例dna样本的方法
CN108929901A (zh) * 2018-07-27 2018-12-04 博奥生物集团有限公司 一种用于痕量dna的多重扩增建库方法及其专用试剂盒
CN109996876A (zh) * 2016-08-22 2019-07-09 特韦斯特生物科学公司 从头合成的核酸文库
US10844373B2 (en) 2015-09-18 2020-11-24 Twist Bioscience Corporation Oligonucleic acid variant libraries and synthesis thereof
CN112176032A (zh) * 2020-10-16 2021-01-05 广州市达瑞生物技术股份有限公司 一个呼吸道病原体的纳米孔测序建库的引物组合及其应用
US10894242B2 (en) 2017-10-20 2021-01-19 Twist Bioscience Corporation Heated nanowells for polynucleotide synthesis
US10894959B2 (en) 2017-03-15 2021-01-19 Twist Bioscience Corporation Variant libraries of the immunological synapse and synthesis thereof
US10907274B2 (en) 2016-12-16 2021-02-02 Twist Bioscience Corporation Variant libraries of the immunological synapse and synthesis thereof
US10936953B2 (en) * 2018-01-04 2021-03-02 Twist Bioscience Corporation DNA-based digital information storage with sidewall electrodes
US10987648B2 (en) 2015-12-01 2021-04-27 Twist Bioscience Corporation Functionalized surfaces and preparation thereof
CN113046418A (zh) * 2021-04-02 2021-06-29 武汉博越致和生物科技有限公司 一种用于妊娠期糖尿病肠道菌群检测16S rDNA扩增子建库方法
US11185837B2 (en) 2013-08-05 2021-11-30 Twist Bioscience Corporation De novo synthesized gene libraries
US11263354B2 (en) 2016-09-21 2022-03-01 Twist Bioscience Corporation Nucleic acid based data storage
US11332738B2 (en) 2019-06-21 2022-05-17 Twist Bioscience Corporation Barcode-based nucleic acid sequence assembly
US11332740B2 (en) 2017-06-12 2022-05-17 Twist Bioscience Corporation Methods for seamless nucleic acid assembly
US11377676B2 (en) 2017-06-12 2022-07-05 Twist Bioscience Corporation Methods for seamless nucleic acid assembly
US11407837B2 (en) 2017-09-11 2022-08-09 Twist Bioscience Corporation GPCR binding proteins and synthesis thereof
CN115232863A (zh) * 2022-09-03 2022-10-25 杭州联川生物技术股份有限公司 一种基于磁珠的均一化建库试剂盒和方法
US11492665B2 (en) 2018-05-18 2022-11-08 Twist Bioscience Corporation Polynucleotides, reagents, and methods for nucleic acid hybridization
US11492727B2 (en) 2019-02-26 2022-11-08 Twist Bioscience Corporation Variant nucleic acid libraries for GLP1 receptor
US11492728B2 (en) 2019-02-26 2022-11-08 Twist Bioscience Corporation Variant nucleic acid libraries for antibody optimization
US11512347B2 (en) 2015-09-22 2022-11-29 Twist Bioscience Corporation Flexible substrates for nucleic acid synthesis
US11550939B2 (en) 2017-02-22 2023-01-10 Twist Bioscience Corporation Nucleic acid based data storage using enzymatic bioencryption
US11691118B2 (en) 2015-04-21 2023-07-04 Twist Bioscience Corporation Devices and methods for oligonucleic acid library synthesis
US11697668B2 (en) 2015-02-04 2023-07-11 Twist Bioscience Corporation Methods and devices for de novo oligonucleic acid assembly
US12091777B2 (en) 2019-09-23 2024-09-17 Twist Bioscience Corporation Variant nucleic acid libraries for CRTH2
CN118726543A (zh) * 2024-09-03 2024-10-01 北京百迈客生物科技有限公司 微生物扩增子文库自动化构建方法、装置及系统
US12173282B2 (en) 2019-09-23 2024-12-24 Twist Bioscience, Inc. Antibodies that bind CD3 epsilon
US12357959B2 (en) 2018-12-26 2025-07-15 Twist Bioscience Corporation Highly accurate de novo polynucleotide synthesis

Citations (4)

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CN101967476A (zh) * 2010-09-21 2011-02-09 深圳华大基因科技有限公司 一种基于接头连接的DNA PCR-Free标签文库构建方法
CN103045726A (zh) * 2012-11-20 2013-04-17 南方科技大学 对多个混合dna或rna序列进行基因测序的方法及装置
US20130184165A1 (en) * 2012-01-13 2013-07-18 Data2Bio Genotyping by next-generation sequencing
CN104153004A (zh) * 2014-08-11 2014-11-19 上海美吉生物医药科技有限公司 一种用于扩增子测序的建库方法

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101967476A (zh) * 2010-09-21 2011-02-09 深圳华大基因科技有限公司 一种基于接头连接的DNA PCR-Free标签文库构建方法
US20130184165A1 (en) * 2012-01-13 2013-07-18 Data2Bio Genotyping by next-generation sequencing
CN103045726A (zh) * 2012-11-20 2013-04-17 南方科技大学 对多个混合dna或rna序列进行基因测序的方法及装置
CN104153004A (zh) * 2014-08-11 2014-11-19 上海美吉生物医药科技有限公司 一种用于扩增子测序的建库方法

Cited By (48)

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US11452980B2 (en) 2013-08-05 2022-09-27 Twist Bioscience Corporation De novo synthesized gene libraries
US11185837B2 (en) 2013-08-05 2021-11-30 Twist Bioscience Corporation De novo synthesized gene libraries
US11559778B2 (en) 2013-08-05 2023-01-24 Twist Bioscience Corporation De novo synthesized gene libraries
US11697668B2 (en) 2015-02-04 2023-07-11 Twist Bioscience Corporation Methods and devices for de novo oligonucleic acid assembly
US11691118B2 (en) 2015-04-21 2023-07-04 Twist Bioscience Corporation Devices and methods for oligonucleic acid library synthesis
US10844373B2 (en) 2015-09-18 2020-11-24 Twist Bioscience Corporation Oligonucleic acid variant libraries and synthesis thereof
US11807956B2 (en) 2015-09-18 2023-11-07 Twist Bioscience Corporation Oligonucleic acid variant libraries and synthesis thereof
US11512347B2 (en) 2015-09-22 2022-11-29 Twist Bioscience Corporation Flexible substrates for nucleic acid synthesis
US11248228B2 (en) 2015-11-30 2022-02-15 Nanjing Annoroad Gene Technology Co. Ltd Method for constructing next-generation sequencing library for detection of lowfrequency mutation and kit thereof
CN106811460A (zh) * 2015-11-30 2017-06-09 安诺优达基因科技(北京)有限公司 用于低频突变检测的二代测序文库的构建方法及试剂盒
US10987648B2 (en) 2015-12-01 2021-04-27 Twist Bioscience Corporation Functionalized surfaces and preparation thereof
CN105779437A (zh) * 2016-03-11 2016-07-20 台州黄岩圣庭医学检验所 一种基于Ion PGMTM测序平台的扩增子DNA文库的构建方法、试剂及其应用
CN107058573A (zh) * 2016-06-13 2017-08-18 艾吉泰康生物科技(北京)有限公司 一种利用Cas9/gRNA系统构建扩增子文库的方法
WO2017219511A1 (zh) * 2016-06-22 2017-12-28 杭州杰毅麦特医疗器械有限公司 一种快速均一化或等比例dna样本的方法
CN109996876A (zh) * 2016-08-22 2019-07-09 特韦斯特生物科学公司 从头合成的核酸文库
US10975372B2 (en) 2016-08-22 2021-04-13 Twist Bioscience Corporation De novo synthesized nucleic acid libraries
CN106282177A (zh) * 2016-08-23 2017-01-04 厦门基源医疗科技有限公司 一种16S rDNA高通量测序文库的构建方法
US11562103B2 (en) 2016-09-21 2023-01-24 Twist Bioscience Corporation Nucleic acid based data storage
US11263354B2 (en) 2016-09-21 2022-03-01 Twist Bioscience Corporation Nucleic acid based data storage
US12056264B2 (en) 2016-09-21 2024-08-06 Twist Bioscience Corporation Nucleic acid based data storage
CN106835291A (zh) * 2016-12-14 2017-06-13 广州燃石医学检验所有限公司 Dna 文库的制备方法以及试剂盒
US10907274B2 (en) 2016-12-16 2021-02-02 Twist Bioscience Corporation Variant libraries of the immunological synapse and synthesis thereof
US11550939B2 (en) 2017-02-22 2023-01-10 Twist Bioscience Corporation Nucleic acid based data storage using enzymatic bioencryption
US10894959B2 (en) 2017-03-15 2021-01-19 Twist Bioscience Corporation Variant libraries of the immunological synapse and synthesis thereof
US11332740B2 (en) 2017-06-12 2022-05-17 Twist Bioscience Corporation Methods for seamless nucleic acid assembly
US11377676B2 (en) 2017-06-12 2022-07-05 Twist Bioscience Corporation Methods for seamless nucleic acid assembly
US12270028B2 (en) 2017-06-12 2025-04-08 Twist Bioscience Corporation Methods for seamless nucleic acid assembly
US11407837B2 (en) 2017-09-11 2022-08-09 Twist Bioscience Corporation GPCR binding proteins and synthesis thereof
US11745159B2 (en) 2017-10-20 2023-09-05 Twist Bioscience Corporation Heated nanowells for polynucleotide synthesis
US10894242B2 (en) 2017-10-20 2021-01-19 Twist Bioscience Corporation Heated nanowells for polynucleotide synthesis
US12086722B2 (en) 2018-01-04 2024-09-10 Twist Bioscience Corporation DNA-based digital information storage with sidewall electrodes
US10936953B2 (en) * 2018-01-04 2021-03-02 Twist Bioscience Corporation DNA-based digital information storage with sidewall electrodes
US11492665B2 (en) 2018-05-18 2022-11-08 Twist Bioscience Corporation Polynucleotides, reagents, and methods for nucleic acid hybridization
US11732294B2 (en) 2018-05-18 2023-08-22 Twist Bioscience Corporation Polynucleotides, reagents, and methods for nucleic acid hybridization
CN108929901B (zh) * 2018-07-27 2022-02-01 博奥生物集团有限公司 一种用于痕量dna的多重扩增建库方法及其专用试剂盒
CN108929901A (zh) * 2018-07-27 2018-12-04 博奥生物集团有限公司 一种用于痕量dna的多重扩增建库方法及其专用试剂盒
US12357959B2 (en) 2018-12-26 2025-07-15 Twist Bioscience Corporation Highly accurate de novo polynucleotide synthesis
US11492728B2 (en) 2019-02-26 2022-11-08 Twist Bioscience Corporation Variant nucleic acid libraries for antibody optimization
US11492727B2 (en) 2019-02-26 2022-11-08 Twist Bioscience Corporation Variant nucleic acid libraries for GLP1 receptor
US12331427B2 (en) 2019-02-26 2025-06-17 Twist Bioscience Corporation Antibodies that bind GLP1R
US11332738B2 (en) 2019-06-21 2022-05-17 Twist Bioscience Corporation Barcode-based nucleic acid sequence assembly
US12091777B2 (en) 2019-09-23 2024-09-17 Twist Bioscience Corporation Variant nucleic acid libraries for CRTH2
US12173282B2 (en) 2019-09-23 2024-12-24 Twist Bioscience, Inc. Antibodies that bind CD3 epsilon
CN112176032A (zh) * 2020-10-16 2021-01-05 广州市达瑞生物技术股份有限公司 一个呼吸道病原体的纳米孔测序建库的引物组合及其应用
CN112176032B (zh) * 2020-10-16 2021-10-26 广州市达瑞生物技术股份有限公司 一个呼吸道病原体的纳米孔测序建库的引物组合及其应用
CN113046418A (zh) * 2021-04-02 2021-06-29 武汉博越致和生物科技有限公司 一种用于妊娠期糖尿病肠道菌群检测16S rDNA扩增子建库方法
CN115232863A (zh) * 2022-09-03 2022-10-25 杭州联川生物技术股份有限公司 一种基于磁珠的均一化建库试剂盒和方法
CN118726543A (zh) * 2024-09-03 2024-10-01 北京百迈客生物科技有限公司 微生物扩增子文库自动化构建方法、装置及系统

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