CN104560634A - Immobilized microalgae breeding method and device thereof - Google Patents
Immobilized microalgae breeding method and device thereof Download PDFInfo
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Abstract
The invention provides an immobilized microalgae breeding method and a device thereof, belongs to the field of microalgae breeding and can be used for effectively controlling the temperature in the microalgae breeding process so as to facilitate the normal growth of microalgae. The immobilized microalgae breeding method comprises the following steps: providing at least one container; inoculating microalgae on the surface of the container or fixedly inoculating the microalgae on a breeding carrier and then paving the breeding carrier on the outer surface of the container; supplying nutrient solution to the microalgae on the outer surface of the container; adding condensate in the container and carrying out indirect heat exchange on the condensate and the microalgae. The immobilized microalgae breeding method and the device thereof can be applied to the immobilized breeding of microalgae.
Description
Technical field
The present invention relates to field of microalgae cultivation, particularly relate to a kind of micro-algae immobilization cultural method and device thereof.
Background technology
Micro-algae immobilization cultural technique refers to be fixed free microalgae cell or is embedded on carrier, cell is made to be in the state of geo-stationary, and culturing liquid has a kind of breeding way of relative flow state, have relative to traditional both culturing microalgae that cost of gathering is low, phototranstormation efficiency advantages of higher.But illumination not only provides the Photosynthetic Energy needed for growth for micro-algae, micro-algae temperature also can be made to raise.Although Immobilized culture improves phototranstormation efficiency, also make temperature in microalga cultivation process significantly raise thus, even exceed the temperature that micro-algae normal growth can tolerate, therefore microalga cultivation process needs to carry out certain control to temperature.
During micro-algae Immobilized culture of existing stage, general is all directly apply phlegma by modes such as sprinklings to culture apparatus and reach the object of cooling, but phlegma directly contacts with micro-algae in temperature-fall period, if maintain the temperature that micro algae growth is suitable, usually the liquid flow rate that maintenance one is larger is needed, but this easily causes the microalgae cell be attached on carrier to run off, be unfavorable for Immobilized culture, if and maintain a less liquid flow rate, in order to the suitable temperature of micro algae growth can be maintained, then require that the temperature of phlegma is very low, and this can cause low temperature injury to the microalgae cell be attached on carrier, suppress micro algae growth.
Therefore, provide one effectively can control micro-algae temperature, guarantee that micro-algae immobilization cultivation apparatus of micro-algae normal growth is the important topic that those skilled in the art face.
Summary of the invention
Embodiments provide a kind of micro-algae immobilization cultural method and device, effectively can control the temperature in microalga cultivation process, be conducive to the normal growth of micro-algae.
For achieving the above object, embodiments of the invention adopt following technical scheme:
The invention provides a kind of micro-algae immobilization cultural method, comprising:
At least one container is provided;
Inoculate micro-algae at container outer surface or micro-algae is fixedly seeded in after on cultivation carrier and cultivation carrier is layed in container outer surface;
For micro-algae supplying nutrition liquid of container outer surface;
Add phlegma at internal tank, phlegma and micro-algae carry out indirect heat exchange.
Wherein, regulate the volume of phlegma, make micro-algae maintain temperature range needed for normal growth.
Particularly, the volume controlling container is more than or equal to the volume of the phlegma micro-algae being maintained the temperature range needed for normal growth;
Inoculate micro-algae at the outside surface with phlegma position cell therefor, or micro-algae is fixedly seeded in cultivation carrier on after cultivation carrier be layed in container outer surface.
Preferably, temperature range needed for micro-algae normal growth that phlegma and micro-algae noncontact heat exchange will reach, obtain the minimum volume of phlegma, the volume arranging container is more than or equal to the volume of the phlegma micro-algae being maintained the temperature range needed for normal growth.
Alternatively, in the above-mentioned methods, specifically can comprise:
The start volume arranging container is the first volume,
According to the change of optical radiation energy, change the volume of container.
Preferably, for the change according to optical radiation energy, change the volume of container, be specially:
Determine average of the whole year light radiation energy;
According to average of the whole year light radiation energy, determine that the volume of container is the first volume V
1;
When light radiation energy is higher than average of the whole year light radiation energy, the volume arranging container is greater than the first volume V
1;
If light radiation energy is lower than when equaling average of the whole year light radiation energy, the volume arranging container equals the first volume V
1; Or
According to phlegma actual received to light radiation energy and the difference of average of the whole year light radiation energy draw the required phlegma volume reduced, the volume arranging container is less than the first volume V
1.
Preferably, for the change according to optical radiation energy, change the volume of container, can also be:
Determine the quantity of radiant energy of first day;
According to the quantity of radiant energy of first day, determine that the volume of the container of first day is the first volume V
2;
If the quantity of radiant energy of second day is higher than the quantity of radiant energy of first day, the volume arranging container is greater than the first volume V
2;
If the quantity of radiant energy of second day is lower than the quantity of radiant energy equaling first day, the volume arranging container equals described first volume V
2, or draw the volume of the required phlegma reduced according to the difference of quantity of radiant energy, the volume arranging container is less than the first volume V
2.
Alternatively, at internal tank sandwich establishment, described interlayer is divided into several sub-folder layers, controls the volume of phlegma according to the quantity of sub-folder layer, micro-algae to be maintained the temperature range needed for normal growth;
Inoculating micro-algae with the sub-folder layer cell therefor outside surface accommodating phlegma, or micro-algae is fixedly seeded in cultivation carrier on after cultivation carrier be layed in container outer surface.
Alternatively, regulate the temperature of phlegma, maintain temperature range needed for normal growth to make micro-algae.
Particularly, in container, arrange temperature control device, this temperature control device controls the temperature of phlegma.
Alternatively, the phlegma in container is changed according to micro-algae temperature maintained needed for normal growth.
Particularly, outside container, arrange temperature control device, circulate described phlegma between container and temperature control device, and this temperature control device regulates the temperature of phlegma.
Preferably, the temperature of phlegma is regulated by temperature control device, Ke Yiwei:
Temperature control device measures condensate temperature and obtains feedback;
Minimum temperature T needed for micro-algae normal growth is provided
1with top temperature T
2;
When condensate temperature close to and higher than minimum temperature T
1time, temperature control device starts the temperature that heating schedule raises phlegma;
When condensate temperature close to and lower than top temperature T
2time, temperature control device starts the temperature that refrigerant sequence reduces phlegma.
Preferably, regulated the temperature of phlegma by temperature control device, can also be:
Temperature control device measures condensate temperature and obtains feedback,
Fixed temperature T is provided
3phlegma, fixed temperature T
3be in the temperature range of micro-algae maintenance needed for normal growth,
When condensate temperature is lower than fixed temperature T
3time, temperature control device starts heating schedule makes condensate temperature raise;
When condensate temperature is higher than fixed temperature T
3time, temperature control device starts refrigerant sequence makes condensate temperature reduce.
Correspondingly, the present invention also provides a kind of micro-algae immobilization cultivation apparatus, comprising:
At least one container;
Container outer surface is directly distributed with micro-algae or is distributed with cultivation carrier, and cultivation carrier is distributed with micro-algae;
Internal tank has phlegma.
Wherein, internal tank is provided with interlayer, and described interlayer comprises sub-folder layer.
Preferably, container is made up of the material that thermal conductivity is high.
Alternatively, the shape of container is cubes, taper or semisphere.
Embodiments provide a kind of micro-algae immobilization cultural method, in the method, phlegma is added with in the inside of container, by the indirect heat exchange between phlegma and micro-algae, can the temperature of micro-algae frond be maintained in the temperature range needed for its normal growth, to realize the object to both culturing microalgae temperature-controllable.
Accompanying drawing explanation
The schematic flow sheet of micro-algae immobilization cultural method that Fig. 1 provides for the embodiment of the present invention;
The structural representation of micro-algae immobilization cultivation apparatus that Fig. 2 provides for the embodiment of the present invention.
Embodiment
Below in conjunction with the accompanying drawing in the embodiment of the present invention, be clearly and completely described the technical scheme in the embodiment of the present invention, obviously, described embodiment is only the present invention's part embodiment, instead of whole embodiments.Based on the embodiment in the present invention, those of ordinary skill in the art, not making the every other embodiment obtained under creative work prerequisite, belong to the scope of protection of the invention.
Below in conjunction with accompanying drawing, the embodiment of the present invention is described in detail.
As shown in Figure 1, the embodiment of the present invention provides a kind of micro-algae immobilization cultural method, specifically can comprise:
S1, provide at least one container;
S2, container outer surface inoculate micro-algae or micro-algae is fixedly seeded in cultivation carrier on after cultivation carrier be layed in container outer surface;
S3, micro-algae supplying nutrition liquid for container outer surface;
S4, internal tank add phlegma, phlegma and micro-algae carry out indirect heat exchange.
Embodiments provide a kind of micro-algae immobilization cultural method, in the method, phlegma is added with in the inside of container, by the indirect heat exchange between phlegma and micro-algae, can the temperature of micro-algae frond be maintained in the temperature range needed for its normal growth, to realize the object to both culturing microalgae temperature-controllable.
In an embodiment of the present invention, by regulating the volume of phlegma, make the temperature range of micro-algae maintenance needed for normal growth.
Utilize the heat transfer effect between phlegma and frond, condensate temperature is raised and makes frond temperature reduce simultaneously, final both can reach an equilibrium temperature.When phlegma specific heat capacity is determined, the volume reaching the phlegma needed for equilibrium temperature can be determined.By adjusting the volume of phlegma, can reach cultivation frond temperature controlled object, thus ensure the top temperature that equilibrium temperature can not tolerate higher than micro algae growth and be not less than the minimum temperature that micro algae growth can tolerate.
In another embodiment of the invention, the volume controlling container is more than or equal to the volume of the phlegma micro-algae being maintained the temperature range needed for normal growth; Inoculating micro-algae with phlegma position cell therefor outside surface, or micro-algae is fixedly seeded in cultivation carrier on after cultivation carrier be layed in container outer surface.
Can by regulating the volume of container to regulate the volume of phlegma.Under normal circumstances, the volume of container is fixing, the volume of phlegma is the volume of container, namely, needing the phlegma of how many volumes in the temperature range maintaining needed for normal growth for making micro-algae, just can set the container with much volumes, now, phlegma is full of whole container, and phlegma is corresponding with the whole outside surface of container.
Also can be, the volume controlling container is greater than the volume of the phlegma micro-algae being maintained the temperature range needed for normal growth, the volume of container has certain excess room relative to the volume of the phlegma making micro-algae maintain needed for optimal temperature, be equivalent to the volume adding the phlegma that can be added in container, like this, the volume of phlegma is not limited to the volume of container, if when causing micro-algae temperature anomaly to raise in breeding process due to abnormal conditions, the volume of the phlegma of actual interpolation can be regulated to tackle abnormal conditions.Now, inoculate micro-algae at the outside surface of phlegma position cell therefor, or micro-algae is fixedly seeded in cultivation carrier on after cultivation carrier be layed in container outer surface, be conducive to the indirect heat exchange between phlegma and micro-algae.
In an alternative embodiment of the invention, temperature range needed for micro-algae normal growth that phlegma and micro-algae indirect heat exchange will reach, obtain the minimum volume of phlegma, the volume arranging container is more than or equal to the volume of the phlegma micro-algae being maintained the temperature range needed for normal growth.
Concrete method to set up is as described below:
The equilibrium temperature reach phlegma and micro-algae heat exchange and the difference of phlegma starting temperature are set to Δ T, for ensureing that the temperature of cultivation system maintains equilibrium temperature, the light radiation energy sponged needed for phlegma is set to Δ Q, and cultivation area cultivates the highest optical radiation energy that in season, odd-numbered day ground can receive and is set to Q
1, the energy corresponding to the top temperature that micro algae growth can tolerate is set to Q
2, the volume of container inner condensat liquid is set to V
min, the density of phlegma is set to ρ, and the specific heat capacity of phlegma is set to c, and equilibrium temperature is less than the top temperature that micro algae growth can tolerate,
So each value Δ T, Δ Q, Q
1, Q
2, V
min, ρ, c should meet following relation,
V
min＝ΔQ/(c·ΔT·ρ)＝(Q
1-Q
2)/(c·ΔT·ρ)。
Wherein, V
minbe the minimum volume of phlegma needed for the temperature range that to be maintained by micro-algae needed for normal growth, this volume is also the minimum volume of container, and the volume arranging container is more than or equal to V
min, can guarantee that container can hold enough phlegmas, the top temperature that can not tolerate higher than micro algae growth with the equilibrium temperature ensured after phlegma and micro-algae generation heat exchange is also not less than the minimum temperature that micro algae growth can tolerate.
It should be noted that, the temperature that micro-algae maintains needed for normal growth has Schwellenwert and maximum, correspondingly, in order to the volume micro-algae being maintained the phlegma needed for the temperature needed for normal growth also has minimum value and maximum value, therefore, the volume that container is set be more than or equal to micro-algae is maintained the volume of the phlegma of the temperature range needed for normal growth time, be not the volume unrestrictedly increasing container.
In an alternative embodiment of the invention, can make adjustment according to the volume of the practical situation of breeding process to container, specifically can comprise:
The start volume arranging container is the first volume; According to the change of optical radiation energy, change the volume of container.
When the specific heat capacity, density etc. of phlegma are determined, the volume change of phlegma is drawn according to the corresponding relation between optical radiation energy and phlegma volume, the change of vessel volume is drawn again by the volume of phlegma, thus vessel volume is made adjustment, such as, equilibrium temperature phlegma and micro-algae reached by heat exchange and the difference of phlegma starting temperature are set to Δ T, for ensureing that the temperature of cultivation system maintains equilibrium temperature, when optical radiation energy changes, temperature difference T is caused to change, according to Δ V=(Q
1-Q
2)/(c Δ T ρ), the volume of container can be the first volume ± Δ V, namely correspondingly can change the volume of container, thus changes the volume of phlegma, and then the heat trnasfer between adjustment phlegma and micro-algae, makes micro-algae maintain suitable temperature.
By the change according to optical radiation energy, vessel volume is adjusted, in unit cultured area, container effectively can be regulated the utilization ratio of cultivation floor space, adjust the quantity of container in cultivating system flexibly.
In a preferred embodiment, according to the change of optical radiation energy in annual breeding process, the volume of container can be changed, specifically can comprise:
Determine average of the whole year light radiation energy;
According to average of the whole year light radiation energy, determine that the volume of container is the first volume V
1;
When light radiation energy is higher than average of the whole year light radiation energy, the volume that can arrange container is greater than the first volume V
1;
At light radiation energy lower than when equaling average of the whole year light radiation energy, the volume that can arrange container equals the first volume V
1; Or
Can according to phlegma actual received to light radiation energy and the difference of average of the whole year light radiation energy draw the required phlegma volume reduced, the volume arranging container is less than the first volume V
1.
In annual breeding process, along with the change in season and month, light radiation energy changes thereupon, and the light radiation energy of the actual reception of phlegma also changes thereupon.Consider this change, corresponding adjustment can be made to vessel volume, to ensure that the volume of vessel volume and phlegma matches, thus guarantee that container can hold the phlegma of appropriate volume, realize the object to microalga cultivation process temperature-controllable.
Concrete adjustment process can be:
Equilibrium temperature phlegma and micro-algae reached by heat exchange and the difference of phlegma starting temperature are set to Δ T, for ensureing that the temperature of cultivation system maintains equilibrium temperature, the light radiation energy sponged needed for phlegma is set to Δ Q, and cultivation area cultivates the highest optical radiation energy that in season, odd-numbered day ground can receive and is set to Q
1, the energy corresponding to the top temperature that micro algae growth can tolerate is set to Q
2, the volume of container inner condensat liquid is set to Vmin, and the density of phlegma is set to ρ, and the specific heat capacity of phlegma is set to c, and equilibrium temperature is less than the top temperature that micro algae growth can tolerate,
So each value Δ T, Δ Q, Q
1, Q
2, Vmin, ρ, c should meet following relation,
Vmin＝ΔQ/(c·ΔT·ρ)＝(Q1-Q2)/(c·ΔT·ρ)；
The equilibrium temperature reach phlegma and micro-algae heat exchange and the difference of phlegma starting temperature are set to Δ T, and for ensureing that the temperature of cultivation system maintains equilibrium temperature, phlegma cultivates the average light sponged needed in season and is set to Δ Q according to quantity of radiant energy
on average, the average photon radiation energy that cultivation odd-numbered day in season ground, cultivation area can receive is set to Q
3, the energy corresponding to the top temperature that micro algae growth can tolerate is set to Q
2, the volume of housing inner condensat liquid is set to V
on average, the density of phlegma is set to ρ, and the specific heat capacity of phlegma is set to c, and equilibrium temperature is less than the top temperature that micro algae growth can tolerate,
So each value Δ T, Δ Q
on average, Q
3, Q
2, V
on average, ρ, c should meet following relation,
V
on average=Δ Q
on average/ (c Δ T ρ)=(Q
3-Q
2)/(c Δ T ρ);
When light radiation energy is higher than average of the whole year light radiation energy, the volume that can arrange container is greater than the first volume V
1, preferably, can according to phlegma actual received to light radiation energy and the difference of average of the whole year light radiation energy draw the required phlegma volume increased, the volume arranging described container is V
1+ Δ V;
At light radiation energy lower than when equaling average of the whole year light radiation energy, then can the volume of uncomfortable lift face device, namely the volume of container equals the first volume V
1; Or
According to phlegma actual received to light radiation energy and the difference of average of the whole year light radiation energy draw the required phlegma volume reduced, the volume arranging container is less than the first volume V
1, preferably, the volume arranging container is V
1-Δ V.
In another preferred embodiment of the present invention, according to the change of optical radiation energy in continuous breeding process, the volume of container can be changed, specifically can comprise:
Determine the quantity of radiant energy of first day;
According to the quantity of radiant energy of first day, determine that the volume of the container of first day is the first volume V
2;
If the quantity of radiant energy of second day is higher than the quantity of radiant energy of first day, the volume arranging container is greater than the first volume V
2;
If the quantity of radiant energy of second day is lower than the quantity of radiant energy equaling first day, the volume arranging container equals described first volume, or draw the volume of the required phlegma reduced according to the difference of quantity of radiant energy, the volume arranging container is less than the first volume V
2.
Namely, draw the difference DELTA V of the volume of required phlegma according to the difference DELTA Q of quantity of radiant energy, be the difference DELTA V of the vessel volume of required adjustment, if the quantity of radiant energy of second day is higher than the quantity of radiant energy of first day, the volume arranging container is greater than the first volume V
2, preferably, can draw the volume of the required phlegma increased according to the difference of quantity of radiant energy, the volume arranging container is V
2+ Δ V; If the quantity of radiant energy of second day is lower than the quantity of radiant energy equaling first day, the volume that can arrange container is V
2, namely keep the constancy of volume of container, or can draw the volume of the required phlegma reduced according to the difference of quantity of radiant energy, the volume arranging container is V
2-Δ V.
In an alternative embodiment of the invention, at internal tank sandwich establishment, described interlayer can be divided into several sub-folder layers, control the volume of phlegma according to the quantity of sub-folder layer, micro-algae to be maintained the temperature range needed for normal growth; Inoculating micro-algae with the sub-folder layer cell therefor outside surface accommodating phlegma, or micro-algae is fixedly seeded in cultivation carrier on after cultivation carrier be layed in container outer surface.
Be understandable that, interlayer can be the grid-like of separation, and namely interlayer is separated into grid-like multiple sub-folder layers.The volume of each sub-folder layer can be equal, also can not wait.
By at internal tank sandwich establishment, described interlayer is divided into several sub-folder layers, controls the volume of phlegma according to the quantity of sub-folder layer, micro-algae to be maintained the temperature range needed for normal growth, specifically comprise:
First determine micro-algae to maintain the volume of phlegma required in the temperature range needed for normal growth; The cubic capacity of sandwich establishment is greater than the volume of phlegma, and the division of interlayer equality or gradient are divided into several sub-folder layers; Determine the quantity of required sub-folder layer according to the volume of phlegma, namely need to add phlegma in how many in interlayer interlayers; Like this, namely by controlling the quantity of sub-folder layer and then controlling the volume of phlegma.In order to obtain better culture efficiency, the sub-folder layer accommodating phlegma is adjacent, and parallel with a certain outside surface of container, and preferentially from the sub-folder layer being adjacent to this outside surface, fill phlegma, be inoculated on the outside surface corresponding to the sub-folder layer being added with phlegma by micro-algae, or micro-algae is fixedly seeded in cultivation carrier on after cultivation carrier be layed on this outside surface.
It should be noted that, inoculate to have the outside surface of micro-algae or micro-algae is fixedly seeded in after on cultivation carrier and this outside surface that cultivation carrier is laid in the above be can be regarded as cultivation face, in order to can better micro-algae be maintained in the temperature range needed for normal growth, no matter need to arrange several sub-folder layer in interlayer, in the sub-folder layer corresponding to cultivation face, phlegma must be filled with.Particularly, for ensureing phlegma to be filled with in the sub-folder layer corresponding to cultivation face, when needing to arrange multiple sub-folder layer in interlayer, from the sub-folder layer corresponding to cultivation face, in adjacent next son interlayer, phlegma is filled successively.
Alternatively, the volume of each sub-folder layer can be set according to optical radiation energy change.Such as, in breeding process, if the change difference of optical radiation energy is Δ Q, then the volume of each sub-folder layer can be set to V=Δ Q/ (c Δ T ρ), particularly, in continuous breeding process:
When the optical radiation energy of optical radiation energy one day after higher than the day before yesterday, the quantity of sub-folder layer can be increased; When the optical radiation energy of optical radiation energy one day after lower than the day before yesterday, the quantity of sub-folder layer can be reduced, like this, just can according to regulating the volume accommodating the quantity regulating phlegma of the interlayer of phlegma, micro-algae to be maintained the temperature range needed for normal growth, can also guarantee that cultivation face is corresponding with the sub-folder layer accommodating phlegma simultaneously, obtain better heat-transfer effect, to obtain better culture efficiency.
In still another embodiment of the process, regulate the temperature of phlegma, maintain temperature range needed for normal growth to make micro-algae.
When regulating the temperature of phlegma, under normal circumstances, the volume that can maintain phlegma is fixing, like this, when the specific heat capacity of phlegma and density are determined, the temperature of phlegma can be regulated according to T=Δ Q/ (cV ρ)=(Q1-Q2)/(cV ρ), thus ensure that the heat exchange between phlegma and micro-algae can ensure that micro-algae maintains normal required temperature.
In a preferred embodiment, in container, arrange temperature control device, this temperature control device controls the temperature of phlegma.
By arranging temperature control device in container, directly temperature control is carried out to the phlegma of internal tank, be conducive to the temperature of the phlegma controlling internal tank more directly, faster, thus shortening phlegma and micro-algae carry out the time that heat exchange reaches balance, and then be conducive to the control of realization to micro-algae temperature fast.Regulated the temperature of phlegma by temperature control device, specifically can comprise two kinds of modes,
Mode one:
Temperature control device measures condensate temperature and obtains feedback;
Minimum temperature T needed for micro-algae normal growth is provided
1with top temperature T
2;
When condensate temperature close to and higher than T
1time, temperature control device starts the temperature that heating schedule raises phlegma;
When condensate temperature close to and lower than T
2time, temperature control device starts the temperature that refrigerant sequence reduces phlegma.
The optimal temperature that micro-algae maintains needed for normal growth has certain scope, and its lower value is minimum temperature T
1, higher limit is top temperature T
2, when condensate temperature close to and higher than T
1time, namely when the temperature of phlegma is close to lower value, also still between this lower value and higher limit, exceed lower value 0.1,0.2,0.5,0.8,1,2,3 DEG C etc. as set, now, temperature control device starts the temperature that heating schedule raises phlegma; When condensate temperature close to and lower than T
2time, namely when the temperature of phlegma is close to lower value, also still between this higher limit and lower value, as set lower than higher limit 0.1,0.2,0.5,0.8,1,2,3 DEG C etc., now, temperature control device starts the temperature that refrigerant sequence reduces phlegma.Like this, just can ensure that the temperature of phlegma remains in the Suitable ranges needed for micro-algae always.
Mode two:
Temperature control device measures condensate temperature and obtains feedback;
Fixed temperature T is provided
3phlegma, fixed temperature is in micro-algae and maintains in temperature range needed for normal growth;
When condensate temperature is lower than T
3time, temperature control device starts heating schedule makes condensate temperature raise;
When condensate temperature is higher than T
3time, temperature control device starts refrigerant sequence makes condensate temperature reduce.
Temperature is fixed on a preset temp, this preset temp is the some values in the temperature range needed for micro-algae normal growth, it can be the optimum value that micro-algae maintains needed for normal growth, also can be intermediate value, with this preset value for tension metrics, when the temperature of phlegma is lower than this value, temperature control device starts heating schedule, when the temperature of phlegma is higher than this value, temperature control device starts refrigerant sequence, like this, the temperature of phlegma can be remained at this preset value, namely remain in the temperature range needed for micro-algae normal growth.
In an alternative embodiment of the invention, the phlegma in container is changed according to micro-algae temperature maintained needed for normal growth.Particularly, the temperature of phlegma is measured, if the temperature of phlegma maintains the temperature needed for normal growth higher than micro-algae, discharge this phlegma, be replaced by the new phlegma with lesser temps; If condensate temperature, lower than the temperature maintained needed for normal growth, is discharged this phlegma, is replaced by the new phlegma with comparatively high temps.
In a preferred embodiment, outside container, arrange temperature control device, circulate described phlegma between container and temperature control device, and this temperature control device regulates the temperature of phlegma.
Similarly, regulated the temperature of phlegma by temperature control device, specifically can comprise two kinds of modes:
Temperature control device measures condensate temperature and obtains feedback, 1) with minimum temperature T
1with top temperature T
2for benchmark, start the temperature of temperature control device to phlegma and regulate; Or, 2) with the shareholder's temperature preset for benchmark, start the temperature of temperature control device to phlegma and regulate.
Being understandable that, can be manual hand manipulation or on-line automatic operation to the operation of temperature control device.
In addition, the model, precision etc. of the present invention to temperature control device are also not construed as limiting, as long as the temperature of phlegma of the present invention can be regulated and controled, the embodiment of the present invention is not limited to this, and those skilled in the art according to content disclosed by the invention and general knowledge known in this field or the selection of common technology means or can determine.
Correspondingly, the present invention also provides a kind of micro-algae immobilization cultivation apparatus, as shown in Figure 2, comprising:
At least one container 10;
Container outer surface 11 is directly distributed with micro-algae or is distributed with cultivation carrier, and cultivation carrier is distributed with micro-algae;
Internal tank has phlegma.
Embodiments provide a kind of micro-algae immobilization cultivation apparatus, in the apparatus, phlegma is added with in the inside of container, by the indirect heat exchange between phlegma and micro-algae, can the temperature of micro-algae frond be maintained in the temperature range needed for its normal growth, to realize the object to both culturing microalgae temperature-controllable.
Wherein, as shown in Figure 2, container 10 inside is provided with interlayer 12, interlayer 12 is divided into several sub-folder layers 121, and sub-folder layer can be the grid-like of separation, and namely interlayer 12 is separated into several grid-like sub-folder layers 121, the volume of each sub-folder layer 121 can be equal, also can not wait.
Grid-like sub-folder layer 121 can be set to the strip sub-folder layer paralleled with container outer surface, as shown in Figure 2, each sub-folder layer 121 is parallel to a certain outside surface 11, this outside surface 11 is inoculated micro-algae or is fixedly seeded in by micro-algae after on cultivation carrier cultivation carrier is layed on this outside surface 11.According to the volume of the phlegma maintained needed for micro-algae normal growth, preferentially add phlegma, to make the sub-folder layer accommodating phlegma corresponding with cultivation face 11 from being adjacent to cultivation has first of this outside surface 11 of micro-algae sub-interlayer 121.Be understandable that, sub-folder layer can also be other shapes such as square, curved shape, fold-line-shaped, and the present invention is not construed as limiting this.
In an embodiment of the present invention, container is made up of the material that thermal conductivity is high.The material of high thermal conductivity coefficient can shorten the time needed for the transmission of warm amount, is conducive to reaching good temperature control effect.
In an alternative embodiment of the invention, the shape of container can be any one in cube, taper or semisphere.The present invention is not construed as limiting this, as long as can hold phlegma, having can cultivating microalgae or place the outside surface of cultivation carrier of micro-algae.Preferably, the shape of container can be taper, and its cultivation surface is bank slope formula, and the dead angle of unglazed photograph is more conducive to receive luminous energy.
Be understandable that, container has opening.Opening can be the entrance adding phlegma, is convenient to add phlegma, can also be the import and export be connected with other equipment, can add phlegma, or condensate circulation is used by other equipment.This container also can be exactly open container, and such as, container can be uncovered cubic container, uncoveredly upwards places, and side is used for cultivating microalgae, and when adding phlegma, frond cultivation height is lower than the height of inside reactor phlegma.
It should be noted that, phlegma can be water, also can be other liquid.What phlegma and micro-algae carried out is noncontact heat exchange, and phlegma can be any medium with thermal capacity and thermal conductivity, and the present invention is not construed as limiting this.
Obviously, above-described embodiment is only for clearly example being described, and the restriction not to embodiment.For those of ordinary skill in the field, can also make other changes in different forms on the basis of the above description.Here exhaustive without the need to also giving all embodiments.And thus the apparent change of extending out or variation be still in the protection domain of the invention.
Claims (18)
1. a micro-algae immobilization cultural method, is characterized in that,
At least one container is provided;
Inoculate micro-algae at described container outer surface or micro-algae is fixedly seeded in after on cultivation carrier and described cultivation carrier is layed in described container outer surface;
For micro-algae supplying nutrition liquid of described container outer surface;
Add phlegma at described internal tank, described phlegma and micro-algae carry out indirect heat exchange.
2. micro-algae immobilization cultural method according to claim 1, is characterized in that,
Regulate the volume of described phlegma, make micro-algae maintain temperature range needed for normal growth.
3. micro-algae immobilization cultural method according to claim 2, is characterized in that, the volume controlling described container is more than or equal to the volume of the described phlegma micro-algae being maintained the temperature range needed for normal growth;
Inoculate micro-algae at the described container outer surface corresponding to described phlegma position, or micro-algae is fixedly seeded in cultivation carrier on after described cultivation carrier is layed in described container outer surface.
4. micro-algae immobilization cultural method according to claim 3, is characterized in that,
The volume of the described container of described control is more than or equal to the volume of the described phlegma micro-algae being maintained the temperature range needed for normal growth, is specially:
Temperature range needed for micro-algae normal growth that described phlegma and micro-algae noncontact heat exchange will reach, obtain the minimum volume of described phlegma, the volume arranging described container is more than or equal to the volume of the described phlegma micro-algae being maintained the temperature range needed for normal growth.
5. micro-algae immobilization cultural method according to claim 2, is characterized in that,
The start volume arranging described container is the first volume,
According to the change of optical radiation energy, change the volume of described container.
6. micro-algae immobilization cultural method according to claim 5, is characterized in that,
Determine average of the whole year light radiation energy;
According to average of the whole year light radiation energy, determine that the volume of described container is the first volume V
1;
When light radiation energy is higher than average of the whole year light radiation energy, the volume arranging described container is greater than described first volume V
1;
At light radiation energy lower than when equaling average of the whole year light radiation energy,
The volume arranging described container equals described first volume V
1; Or
According to described phlegma actual received to light radiation energy and the difference of average of the whole year light radiation energy draw the required described phlegma volume reduced, the volume arranging described container is less than described first volume V
1.
7. micro-algae immobilization cultural method according to claim 5, is characterized in that,
Determine the quantity of radiant energy of first day;
According to the quantity of radiant energy of first day, determine that the volume of the described container of first day is the first volume V
2;
When the quantity of radiant energy of the quantity of radiant energy of second day higher than first day, the volume arranging described container is greater than described first volume V
2;
The quantity of radiant energy of second day lower than when equaling the quantity of radiant energy of first day,
The volume arranging described container equals described first volume V
2; Or,
Draw the required described phlegma volume reduced according to the difference of quantity of radiant energy, the volume arranging described container is less than described first volume V
2.
8. micro-algae immobilization cultural method according to claim 2, is characterized in that,
At described internal tank sandwich establishment, described interlayer is divided into several sub-folder layers, controls the volume of described phlegma according to the quantity of described sub-folder layer, micro-algae to be maintained the temperature range needed for normal growth;
Inoculate micro-algae at the described container outer surface corresponding to the described sub-folder layer accommodating described phlegma, or micro-algae is fixedly seeded in cultivation carrier on after described cultivation carrier is layed in described container outer surface.
9. micro-algae immobilization cultural method according to claim 1, is characterized in that, regulate the temperature of described phlegma, makes micro-algae maintain temperature range needed for normal growth.
10. micro-algae immobilization cultural method according to claim 9, is characterized in that,
In described container, arrange temperature control device, described temperature control device controls the temperature of described phlegma.
11. micro-algae immobilization cultural methods according to claim 9, is characterized in that,
Maintain the temperature needed for normal growth according to micro-algae, change the described phlegma in described container.
12. micro-algae immobilization cultural methods according to claim 11, is characterized in that,
Outside described container, arrange temperature control device, circulate described phlegma between described container and described temperature control device, and described temperature control device regulates the temperature of described phlegma.
13. micro-algae immobilization cultural methods according to claim 10 or 12, is characterized in that,
Described temperature control device measures described condensate temperature and obtains feedback;
Minimum temperature T needed for micro-algae normal growth is provided
1with top temperature T
2;
When described condensate temperature close to and higher than minimum temperature T
1time, described temperature control device starts the temperature that heating schedule raises described phlegma;
When described condensate temperature close to and lower than top temperature T
2time, described temperature control device starts the temperature that refrigerant sequence reduces described phlegma.
14. micro-algae immobilization cultural methods according to claim 10 or 12, is characterized in that,
Described temperature control device measures described condensate temperature and obtains feedback,
There is provided and there is fixed temperature T
3described phlegma, described fixed temperature T
3be in the temperature range of micro-algae maintenance needed for normal growth,
When described condensate temperature is lower than described fixed temperature T
3time, described temperature control device starts heating schedule makes described condensate temperature raise;
When described condensate temperature is higher than described fixed temperature T
3time, described temperature control device starts refrigerant sequence makes described condensate temperature reduce.
15. 1 kinds of micro-algae immobilization cultivation apparatus, is characterized in that, comprising:
At least one container;
Described container outer surface is directly distributed with micro-algae or is distributed with cultivation carrier, and described cultivation carrier is distributed with micro-algae;
Described internal tank has phlegma.
16. micro-algae immobilization cultivation apparatus according to claim 15, it is characterized in that, described internal tank is provided with interlayer, and described interlayer comprises sub-folder layer.
17. micro-algae immobilization cultivation apparatus according to claim 15, it is characterized in that, described container is made up of the material that thermal conductivity is high.
18. micro-algae immobilization cultivation apparatus according to claim 15, it is characterized in that, the shape of described container is cube, taper or semisphere.
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| US20120252105A1 (en) * | 2008-10-24 | 2012-10-04 | Bioprocessh20 Llc | Systems, apparatuses and methods of cultivating organisms and mitigation of gases |
| WO2011017171A1 (en) * | 2009-07-28 | 2011-02-10 | Joule Unlimited, Inc. | Photobioreactors, solar energy gathering systems, and thermal control methods |
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