CN104560634B - Immobilized microalgae breeding method and device thereof - Google Patents
Immobilized microalgae breeding method and device thereof Download PDFInfo
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Abstract
The invention provides an immobilized microalgae breeding method and a device thereof, belongs to the field of microalgae breeding and can be used for effectively controlling the temperature in the microalgae breeding process so as to facilitate the normal growth of microalgae. The immobilized microalgae breeding method comprises the following steps: providing at least one container; inoculating microalgae on the surface of the container or fixedly inoculating the microalgae on a breeding carrier and then paving the breeding carrier on the outer surface of the container; supplying nutrient solution to the microalgae on the outer surface of the container; adding condensate in the container and carrying out indirect heat exchange on the condensate and the microalgae. The immobilized microalgae breeding method and the device thereof can be applied to the immobilized breeding of microalgae.
Description
Technical field
The present invention relates to field of microalgae cultivation, particularly relate to a kind of microalgae immobilization cultural method and
Its device.
Background technology
Microalgae immobilization cultural technique refers to free microalgae cell is fixed or is embedded on carrier, makes
Cell is in the state of geo-stationary, and culturing liquid has a kind of breeding way of relative flow regime, phase
For tradition both culturing microalgae, there is low cost of gathering, phototranstormation efficiency advantages of higher.But, illumination is not only
There is provided the Photosynthetic Energy needed for growth for microalgae, microalgae temperature also can be made to raise.Although Immobilized culture carries
High phototranstormation efficiency, but also so that in microalga cultivation process temperature significantly raise, the most micro-
The temperature that algae normal growth is resistant to, therefore microalga cultivation process needs temperature is carried out certain control
System.
During existing stage microalgae Immobilized culture, it is typically all direct to culture apparatus by modes such as sprinklings
Apply condensed fluid and reach the purpose of cooling, but condensed fluid directly contacts with microalgae in temperature-fall period, as
The temperature that fruit micro algae growth to be maintained is suitable, it usually needs maintain a bigger fluid flow, but this is easy
Cause the microalgae cell being attached on carrier to run off, be unfavorable for Immobilized culture, and if maintaining one relatively
Little fluid flow, in order to the temperature that maintenance micro algae growth is suitable, then requires the temperature of condensed fluid very
Low, and the microalgae cell being attached on carrier can be caused low temperature injury by this, suppresses micro algae growth.
Therefore it provides one can effectively control microalgae temperature, it is ensured that the microalgae immobilization of microalgae normal growth
Cultivation apparatus is the important topic that those skilled in the art are faced.
Summary of the invention
Embodiments provide a kind of microalgae immobilization cultural method and device, it is possible to effectively control micro-
The normal growth of the temperature in algae breeding process, beneficially microalgae.
For reaching above-mentioned purpose, embodiments of the invention adopt the following technical scheme that
The present invention provides a kind of microalgae immobilization cultural method, including:
At least one container is provided；
Rear cultivation carrier paving on carrier is cultivated at container outer surface inoculation microalgae or by fixing for microalgae being seeded in
It is located at container outer surface；
Microalgae supplying nutrition liquid for container outer surface；
Adding condensed fluid inside container, condensed fluid and microalgae carry out indirect heat exchange.
Wherein, the volume of regulation condensed fluid, make microalgae maintain the temperature range needed for normal growth.
Specifically, the volume of container is controlled more than or equal to microalgae being maintained the temperature model needed for normal growth
The volume of the condensed fluid enclosed；
Microalgae is inoculated at the outer surface with condensed fluid position cell therefor, or by fixing for microalgae inoculation
After on cultivation carrier, cultivation carrier is layed in container outer surface.
Preferably, needed for according to the microalgae normal growth that condensed fluid and microalgae noncontact heat exchange are to be reached
Temperature range, it is thus achieved that the minimum volume of condensed fluid, the volume of container is set and maintains more than or equal to by microalgae
The volume of the condensed fluid of the temperature range needed for normal growth.
Alternatively, in the above-mentioned methods, specifically may include that
The start volume arranging container is the first volume,
According to the change of optical radiation energy, change the volume of container.
Preferably for the change according to optical radiation energy, change the volume of container, particularly as follows:
Determine average of the whole year light radiation energy；
According to average of the whole year light radiation energy, determine that the volume of container is the first volume V1；
When light radiation energy is higher than average of the whole year light radiation energy, the volume of container is set more than the
One volume V1；
If light radiation energy is less than during equal to average of the whole year light radiation energy, the volume etc. of container is set
In the first volume V1；Or
The light radiation energy arrived according to condensed fluid actual received and the difference of average of the whole year light radiation energy
It is worth the condensed fluid volume of required reduction, the volume of container is set less than the first volume V1。
Preferably for the change according to optical radiation energy, change the volume of container, it is also possible to for:
Determine the emittance of first day；
According to the emittance of first day, determine that the volume of the container of first day is the first volume V2；
If the emittance that the emittance of second day was higher than first day, the volume of container is set more than first
Volume V2；
If the emittance of second day is less than the emittance equal to first day, the volume arranging container is equal to
Described first volume V2, or, the volume of the condensed fluid of required reduction is drawn according to the difference of emittance,
The volume of container is set less than the first volume V2。
Alternatively, at the internal sandwich establishment of container, described interlayer is divided into several sub-folder layers, according to son
The quantity of interlayer controls the volume of condensed fluid, so that microalgae to maintain the temperature range needed for normal growth；
Inoculating microalgae with the sub-folder layer cell therefor outer surface accommodating condensed fluid, or microalgae is being fixed
After being seeded on cultivation carrier, cultivation carrier is layed in container outer surface.
Alternatively, the temperature of regulation condensed fluid, so that microalgae maintains the temperature range needed for normal growth.
Specifically, arranging temperature control device in container, this temperature control device controls the temperature of condensed fluid.
Alternatively, the temperature needed for normal growth is maintained to change the condensed fluid in container according to microalgae.
Specifically, temperature control device is set outside container, between container and temperature control device, circulates described condensation
Liquid, the temperature of this temperature control device regulation condensed fluid.
Preferably, the temperature of condensed fluid is regulated by temperature control device, Ke Yiwei:
Condensate temperature is measured and obtains feedback by temperature control device；
Minimum temperature T needed for microalgae normal growth is provided1With maximum temperature T2；
When condensate temperature is close to and above minimum temperature T1Time, temperature control device starts heating schedule and raises cold
The temperature of lime set；
When condensate temperature is close and is less than maximum temperature T2Time, temperature control device starts refrigerant sequence and reduces cold
The temperature of lime set.
Preferably, by the temperature of temperature control device regulation condensed fluid, it is also possible to for:
Condensate temperature is measured and obtains feedback by temperature control device,
Fixed temperature T is provided3Condensed fluid, fixed temperature T3Needed for being in microalgae maintenance normal growth
In temperature range,
When condensate temperature is less than fixed temperature T3Time, temperature control device starts heating schedule makes condensate temperature
Raise；
When condensate temperature is higher than fixed temperature T3Time, temperature control device starts refrigerant sequence makes condensate temperature
Reduce.
Correspondingly, the present invention also provides for a kind of microalgae immobilization cultivation apparatus, including:
At least one container；
Container outer surface is directly distributed microalgae or cultivation carrier is distributed, and microalgae is distributed on cultivation carrier；
Condensed fluid is had inside container.
Wherein, inside container, it is provided with interlayer, and described interlayer comprises sub-folder layer.
Preferably, container is made up of the material that heat conductivity is high.
Alternatively, container be shaped as cube, taper or hemispherical.
Embodiments provide a kind of microalgae immobilization cultural method, in the method, at container
Inside is added with condensed fluid, by the indirect heat exchange between condensed fluid and microalgae, it is possible to by microalgae algae
The temperature of body maintains within the scope of the temperature needed for its normal growth, to realize both culturing microalgae temperature-controllable
Purpose.
Accompanying drawing explanation
The schematic flow sheet of the microalgae immobilization cultural method that Fig. 1 provides for the embodiment of the present invention；
The structural representation of the microalgae immobilization cultivation apparatus that Fig. 2 provides for the embodiment of the present invention.
Detailed description of the invention
Below in conjunction with the accompanying drawing in the embodiment of the present invention, to the technical side in the embodiment of the present invention
Case is clearly and completely described, it is clear that described embodiment is only the present invention one
Divide embodiment rather than whole embodiments.Based on the embodiment in the present invention, this area is general
The every other embodiment that logical technical staff is obtained under not making creative work premise,
Broadly fall into the scope of protection of the invention.
Below in conjunction with the accompanying drawings the embodiment of the present invention is described in detail.
As it is shown in figure 1, the embodiment of the present invention provides a kind of microalgae immobilization cultural method, specifically
May include that
S1, provide at least one container；
S2, container outer surface inoculation microalgae or by microalgae fixing be seeded on cultivation carrier after handle
Cultivation carrier is layed in container outer surface；
S3, microalgae supplying nutrition liquid for container outer surface；
S4, adding condensed fluid inside container, condensed fluid and microalgae carry out indirect heat exchange.
Embodiments provide a kind of microalgae immobilization cultural method, in the method,
The inside of container is added with condensed fluid, by the indirect heat exchange between condensed fluid and microalgae,
Can the temperature of microalgae frond be maintained within the scope of the temperature needed for its normal growth, to realize
Purpose to both culturing microalgae temperature-controllable.
In an embodiment of the present invention, by regulating the volume of condensed fluid, microalgae is made to maintain normal growth institute
The temperature range needed.
Utilize the heat transfer effect between condensed fluid and frond, make condensate temperature raise and make frond temperature simultaneously
Degree reduces, and final both can reach an equilibrium temperature.In the case of condensed fluid specific heat capacity determines, reach
Volume to the condensed fluid needed for equilibrium temperature i.e. can determine that.By the volume of condensed fluid is adjusted,
Can reach the cultivation temperature controlled purpose of frond, thus ensure that equilibrium temperature is not higher than micro algae growth institute energy
Reach the maximum temperature of tolerance and be not less than the minimum temperature that micro algae growth is resistant to.
In another embodiment of the invention, the volume of container is controlled more than or equal to being maintained normally by microalgae
The volume of the condensed fluid of the temperature range needed for growth；With condensed fluid position cell therefor appearance
Face inoculation microalgae, or after fixing for microalgae being seeded on cultivation carrier, cultivation carrier is layed in container appearance
Face.
Can be by the volume of regulation container to regulate the volume of condensed fluid.Under normal circumstances, the appearance of container
Long-pending is fixing, and the volume of condensed fluid is the volume of container, it is, for making microalgae maintain normally
The condensed fluid of how many volumes is needed, it is possible to set and there is much volumes within the scope of temperature needed for growth
Container, now, condensed fluid is full of whole container, and condensed fluid is corresponding with the whole outer surface of container.
Can also be to control the volume of container more than microalgae being maintained the temperature range needed for normal growth
The volume of condensed fluid, the volume of container maintains condensed fluid needed for preference temperature relative to making microalgae
Volume has certain excess room, is equivalent to add the volume of the condensed fluid that may be added to that in container,
So, the volume of condensed fluid is not limited to the volume of container, if due to abnormal conditions in breeding process
When causing microalgae temperature anomaly to raise, the volume of condensed fluid of actual interpolation can be regulated with the abnormal feelings of reply
Condition.Now, the outer surface at condensed fluid position cell therefor inoculates microalgae, or is fixed by microalgae
After being seeded on cultivation carrier, cultivation carrier is layed in container outer surface, beneficially condensed fluid and microalgae it
Between indirect heat exchange.
In an alternative embodiment of the invention, according to micro-to be reached with microalgae indirect heat exchange of condensed fluid
Temperature range needed for algae normal growth, it is thus achieved that the minimum volume of condensed fluid, the volume arranging container is more than
Volume equal to the condensed fluid of temperature range microalgae maintained needed for normal growth.
Concrete method to set up is as described below:
The equilibrium temperature reached with microalgae heat exchange by condensed fluid and the difference of condensed fluid initial temperature are set to
Δ T, for ensureing that the temperature of cultivation system maintains the light radiation sponged needed for equilibrium temperature, condensed fluid
Energy is set to Δ Q, and cultivation area cultivates the highest optical radiation energy that in season, odd-numbered day ground is able to receive that
It is set to Q1, the energy corresponding to maximum temperature that micro algae growth is resistant to is set to Q2, condensation in container
The volume of liquid is set to Vmin, the density of condensed fluid is set to ρ, and the specific heat capacity of condensed fluid is set to c, equilibrium temperature
The maximum temperature being resistant to less than micro algae growth,
So each value Δ T, Δ Q, Q1、Q2、Vmin, ρ, c should meet following relation,
Vmin=Δ Q/ (c Δ T ρ)=(Q1-Q2)/(c·ΔT·ρ)。
Wherein, VminIt is and microalgae is maintained needed for the temperature range needed for normal growth condensed fluid
Small size, this volume is also the minimum volume of container, arranges the volume of container more than or equal to Vmin, can be true
Protect container and can accommodate enough condensed fluid, to ensure the balance temperature after condensed fluid and microalgae generation heat exchange
The degree maximum temperature that not higher than micro algae growth is resistant to also is not less than micro algae growth and is resistant to
Low temperature.
Temperature needed for it should be noted that microalgae maintenance normal growth has minimum and peak, phase
Ying Di, in order to the volume of the condensed fluid needed for the temperature needed for microalgae is maintained normal growth also has
Little value and maximum, therefore, arranging the volume of container more than or equal to microalgae maintains normal growth institute
During the volume of condensed fluid of the temperature range needed, it it is not the volume unrestrictedly increasing container.
In an alternative embodiment of the invention, according to the practical situation of breeding process, the volume of container can be made
Go out to adjust, specifically may include that
The start volume arranging container is the first volume；According to the change of optical radiation energy, change container
Volume.
In the case of the specific heat capacity of condensed fluid, density etc. determine, according to optical radiation energy and condensed fluid
Corresponding relation between Ji draws the change in volume of condensed fluid, then is drawn vessel volume by the volume of condensed fluid
Change, thus vessel volume is made adjustment, such as, condensed fluid is reached by heat exchange with microalgae
Equilibrium temperature and the difference of condensed fluid initial temperature be set to Δ T, for ensureing that the temperature of cultivation system maintains
In equilibrium temperature, when optical radiation energy changes, temperature difference T is caused to change, according to Δ
V=(Q1-Q2)/(c Δ T ρ), the volume of container can be the first volume ± Δ V, i.e. can correspondingly change
The volume of variodenser, thus change the volume of condensed fluid, and then adjust the heat biography between condensed fluid and microalgae
Pass, make microalgae maintain suitable temperature.
By the change according to optical radiation energy, vessel volume is adjusted, in unit cultured area,
Can effectively regulate the container utilization rate to cultivation floor space, in the cultivating system quantity of container is adjusted flexibly.
In a preferred embodiment, can change according to optical radiation energy in annual breeding process,
Change the volume of container, specifically may include that
Determine average of the whole year light radiation energy；
According to average of the whole year light radiation energy, determine that the volume of container is the first volume V1；
When light radiation energy is higher than average of the whole year light radiation energy, the volume that can arrange container is big
In the first volume V1；
At light radiation energy less than during equal to average of the whole year light radiation energy, the appearance of container can be set
Long-pending equal to the first volume V1；Or
The light radiation energy that can arrive according to condensed fluid actual received and average of the whole year light radiation energy
Difference draw the condensed fluid volume of required reduction, the volume of container is set less than the first volume V1。
In annual breeding process, along with season and the change in month, light radiation energy becomes therewith
Changing, the light radiation energy of the actual reception of condensed fluid changes the most therewith.In view of this change, can
Vessel volume is made corresponding adjustment, to ensure that vessel volume matches with the volume of condensed fluid, thus really
Protect container and can accommodate the condensed fluid of appropriate volume, it is achieved the purpose to microalga cultivation process temperature-controllable.
Concrete adjustment process can be:
The equilibrium temperature reached by heat exchange with microalgae by condensed fluid and the difference of condensed fluid initial temperature are set
For Δ T, the temperature for guarantee cultivation system maintains the illumination sponged needed for equilibrium temperature, condensed fluid
Emittance is set to Δ Q, and cultivation area cultivates the highest light radiation that in season, odd-numbered day ground is able to receive that
Energy is set to Q1, the energy corresponding to maximum temperature that micro algae growth is resistant to is set to Q2, in container
The volume of condensed fluid is set to Vmin, and the density of condensed fluid is set to ρ, and the specific heat capacity of condensed fluid is set to c, flat
The maximum temperature that weighing apparatus temperature is resistant to less than micro algae growth,
So each value Δ T, Δ Q, Q1、Q2, Vmin, ρ, c should meet following relation,
Vmin=Δ Q/ (c Δ T ρ)=(Q1-Q2)/(c Δ T ρ)；
The equilibrium temperature reached with microalgae heat exchange by condensed fluid and the difference of condensed fluid initial temperature are set to Δ
T, for ensureing that the temperature of cultivation system maintains equilibrium temperature, in condensed fluid cultivation season needed for sponge
Average light be set to Δ Q according to emittanceAveragely, cultivation odd-numbered day in season ground, cultivation area is able to receive that
Average photon radiation energy is set to Q3, the energy corresponding to maximum temperature that micro algae growth is resistant to is set to
Q2, the volume of housing inner condensat liquid is set to VAveragely, the density of condensed fluid is set to ρ, the specific heat capacity of condensed fluid
It is set to c, the maximum temperature that equilibrium temperature is resistant to less than micro algae growth,
So each value Δ T, Δ QAveragely、Q3、Q2、VAveragely, ρ, c should meet following relation,
VAveragely=Δ QAveragely/ (c Δ T ρ)=(Q3-Q2)/(c·ΔT·ρ)；
When light radiation energy is higher than average of the whole year light radiation energy, the volume that can arrange container is big
In the first volume V1, it is preferable that the light radiation energy that can arrive according to condensed fluid actual received is with annual
Average light draws the required condensed fluid volume increased according to the difference of emittance, arranges the volume of described container
For V1+ΔV；
When light radiation energy is less than equal to average of the whole year light radiation energy, then can be with uncomfortable face-lifting device
Volume, i.e. the volume of container be equal to the first volume V1；Or
The light radiation energy arrived according to condensed fluid actual received and the difference of average of the whole year light radiation energy
It is worth the condensed fluid volume of required reduction, the volume of container is set less than the first volume V1, it is preferable that
The volume arranging container is V1-ΔV。
In another preferred embodiment of the present invention, can change according to optical radiation energy in continuous breeding process
Change, change the volume of container, specifically may include that
Determine the emittance of first day；
According to the emittance of first day, determine that the volume of the container of first day is the first volume V2；
If the emittance that the emittance of second day was higher than first day, the volume of container is set more than first
Volume V2；
If the emittance of second day is less than the emittance equal to first day, the volume arranging container is equal to
Described first volume, or, the volume of the condensed fluid of required reduction is drawn according to the difference of emittance,
The volume of container is set less than the first volume V2。
It is, draw difference DELTA V of the volume of required condensed fluid according to difference DELTA Q of emittance,
It is difference DELTA V of the vessel volume of required adjustment, if the spoke that the emittance of second day was higher than first day
Penetrate energy, the volume of container is set more than the first volume V2, it is preferable that can be according to the difference of emittance
Being worth the volume of the condensed fluid of required increase, the volume arranging container is V2+ΔV；If second day
Emittance is less than the emittance equal to first day, and the volume that can arrange container is V2, i.e. keep holding
The constancy of volume of device, or the volume of the condensed fluid of required reduction can be drawn according to the difference of emittance,
The volume arranging container is V2-ΔV。
In an alternative embodiment of the invention, can be at the internal sandwich establishment of container, if described interlayer is divided into
Dry sub-interlayer, controls the volume of condensed fluid according to the quantity of sub-folder layer, normal raw to be maintained by microalgae
Temperature range needed for length；Inoculating micro-with the sub-folder layer cell therefor outer surface accommodating condensed fluid
Algae, or after fixing for microalgae being seeded on cultivation carrier, cultivation carrier is layed in container outer surface.
It is understood that interlayer can be the grid-like of separation, i.e. interlayer is divided into grid-like many
Individual sub-interlayer.The volume of each sub-folder layer can be equal, it is also possible to.
By at the internal sandwich establishment of container, described interlayer being divided into several sub-folder layers, according to sub-folder layer
Quantity control condensed fluid volume, microalgae is maintained the temperature range needed for normal growth, specifically
Including:
First the body of condensed fluid required within the scope of microalgae is maintained the temperature needed for normal growth is determined
Long-pending；The total measurement (volume) of sandwich establishment is more than the volume of condensed fluid, and the division of interlayer equality or gradient is divided into
Several sub-folder layers；Volume according to condensed fluid determines the quantity of required sub-folder layer, i.e. needs at interlayer
In how many sub-interlayers in add condensed fluid；Such that can be by controlling quantity and then the control of sub-folder layer
The volume of refrigeration lime set.In order to obtain more preferable culture efficiency, the sub-folder layer accommodating condensed fluid is adjacent
, and parallel with a certain outer surface of container, and preferential from the beginning of the sub-folder layer being adjacent to this outer surface
Fill condensed fluid, will microalgae be inoculated in on the corresponding outer surface of sub-folder layer being added with condensed fluid, or
After fixing for microalgae being seeded on cultivation carrier, cultivation carrier is layed on this outer surface.
It should be noted that be inoculated with the outer surface of microalgae or by after fixing for microalgae being seeded on cultivation carrier
This outer surface that cultivation carrier is laid in the above can be regarded as cultivation face, in order to preferably
Microalgae is maintained within the scope of the temperature needed for normal growth, no matter needing to arrange several sub-folder in interlayer
Layer, is necessarily filled with condensed fluid in sub-folder layer corresponding with cultivation face.Specifically, for ensureing and cultivation face
Corresponding sub-folder layer is necessarily filled with condensed fluid, when needing to arrange multiple sub-folder layer in interlayer, from
Cultivation face corresponding sub-folder layer starts, and fills condensed fluid successively in adjacent next son interlayer.
It is alternatively possible to arrange the volume of each sub-folder layer according to optical radiation energy change.Such as, supporting
During growing, if the change difference of optical radiation energy is Δ Q, the volume of the most each sub-folder layer can be arranged
For V=Δ Q/ (c Δ T ρ), specifically, as a example by continuous breeding process:
When optical radiation energy one day after is higher than the optical radiation energy of the previous day, the number of sub-folder layer can be increased
Amount；When optical radiation energy one day after is less than the optical radiation energy of the previous day, the number of sub-folder layer can be reduced
Amount, so, just can accommodate the volume of the quantity regulating condensed fluid of the interlayer of condensed fluid according to regulation, with
Microalgae is maintained the temperature range needed for normal growth, also ensures that cultivation face simultaneously and accommodate condensation
The sub-folder layer of liquid is corresponding, obtains more preferable heat-transfer effect, to obtain more preferable culture efficiency.
In still another embodiment of the process, the temperature of regulation condensed fluid, so that microalgae maintains normal growth
Required temperature range.
When regulating the temperature of condensed fluid, it is generally the case that the volume that can maintain condensed fluid is fixing,
So, in the case of the specific heat capacity of condensed fluid and density determine, can according to T=Δ Q/ (c V ρ)=
(Q1-Q2)/(c V ρ) regulate the temperature of condensed fluid, thus ensure the heat exchange energy between condensed fluid and microalgae
Enough ensure that microalgae maintains normal required temperature.
In a preferred embodiment, arranging temperature control device in container, this temperature control device controls cold
The temperature of lime set.
By arranging temperature control device in container, directly the condensed fluid within container is carried out temperature control,
Be conducive to the most directly, control the temperature of condensed fluid container within faster, thus shorten condensed fluid and
Microalgae carries out heat exchange and reaches the time of balance, and then is conducive to the control quickly realized microalgae temperature.
By the temperature of temperature control device regulation condensed fluid, two ways specifically can be included,
Mode one:
Condensate temperature is measured and obtains feedback by temperature control device；
Minimum temperature T needed for microalgae normal growth is provided1With maximum temperature T2；
When condensate temperature is close to and above T1Time, temperature control device starts heating schedule and raises the temperature of condensed fluid
Degree；
When condensate temperature is close and is less than T2Time, temperature control device starts refrigerant sequence and reduces the temperature of condensed fluid
Degree.
Preference temperature needed for microalgae maintains normal growth has certain scope, and its lower limit is lowest temperature
Degree T1, higher limit is maximum temperature T2, when condensate temperature is close to and above T1Time, namely when cold
The temperature of lime set, close to lower limit, also remains unchanged between this lower limit and higher limit, exceeds as set
Lower limits 0.1,0.2,0.5,0.8,1,2,3 DEG C etc., now, temperature control device starts heating schedule liter
The temperature of high condensed fluid；When condensate temperature is close and is less than T2Time, namely connect when the temperature of condensed fluid
Nearly lower limit, also remains unchanged between this higher limit and lower limit, as can be set lower than higher limit 0.1,0.2,
0.5,0.8,1,2,3 DEG C etc., now, temperature control device starts refrigerant sequence and reduces the temperature of condensed fluid.
So, it becomes possible to ensure that the temperature of condensed fluid is always held in the Suitable ranges needed for microalgae.
Mode two:
Condensate temperature is measured and obtains feedback by temperature control device；
Fixed temperature T is provided3Condensed fluid, fixed temperature is in microalgae and maintains the temperature needed for normal growth
In the range of；
When condensate temperature is less than T3Time, temperature control device starts heating schedule makes condensate temperature raise；
When condensate temperature is higher than T3Time, temperature control device starts refrigerant sequence makes condensate temperature reduce.
Temperature is fixed on a preset temperature, and this preset temperature is the temperature range needed for microalgae normal growth
Some interior value, can be the optimal value needed for microalgae maintenance normal growth, it is also possible to be intermediate value,
With this preset value as tension metrics, when the temperature of condensed fluid is less than this value, temperature control device starts heating journey
Sequence, when the temperature of condensed fluid is higher than this value, temperature control device starts refrigerant sequence, so, can will condense
The temperature of liquid remains at this preset value, i.e. remains at the temperature range needed for microalgae normal growth
In.
In an alternative embodiment of the invention, the temperature needed for normal growth is maintained to change in container according to microalgae
Condensed fluid.Specifically, the temperature of condensed fluid is measured, if the temperature of condensed fluid is tieed up higher than microalgae
Hold the temperature needed for normal growth, discharge this condensed fluid, be replaced by the new condensed fluid with lower temperature；
If condensate temperature is less than the temperature maintained needed for normal growth, discharges this condensed fluid, be replaced by and have relatively
The new condensed fluid of high-temperature.
In a preferred embodiment, temperature control device is set outside container, at container and temperature control device
Between circulate described condensed fluid, this temperature control device regulation condensed fluid temperature.
Similarly, by the temperature of temperature control device regulation condensed fluid, two ways can specifically be included:
Condensate temperature is measured and obtains feedback by temperature control device, and 1) with minimum temperature T1The highest
Temperature T2On the basis of, start temperature control device and the temperature of condensed fluid is adjusted；Or, 2) and to preset
Shareholder's temperature on the basis of, start temperature control device the temperature of condensed fluid is adjusted.
It is understood that the operation to temperature control device can be manual hand manipulation or on-line automatic operation.
Additionally, the model of temperature control device, precision etc. are also not construed as limiting, as long as can adjust by the present invention
Controlling the temperature of condensed fluid of the present invention, the embodiment of the present invention is not limited to this, this area skill
Art personnel can select according to present disclosure and general knowledge known in this field or common technology means
Select or determine.
Correspondingly, the present invention also provides for a kind of microalgae immobilization cultivation apparatus, as in figure 2 it is shown, include:
At least one container 10；
Container outer surface 11 is directly distributed microalgae or cultivation carrier is distributed, and is distributed micro-on cultivation carrier
Algae；
Condensed fluid is had inside container.
Embodiments provide a kind of microalgae immobilization cultivation apparatus, in the apparatus,
The inside of container is added with condensed fluid, by the indirect heat exchange between condensed fluid and microalgae,
Can the temperature of microalgae frond be maintained within the scope of the temperature needed for its normal growth, to realize
Purpose to both culturing microalgae temperature-controllable.
Wherein, as in figure 2 it is shown, container 10 is internal is provided with interlayer 12, interlayer 12 is divided into several sub-folders
Layer 121, sub-folder layer can be the grid-like of separation, i.e. interlayer 12 is divided into grid-like some height
Interlayer 121, the volume of each sub-folder layer 121 can be equal, it is also possible to.
Grid-like sub-folder layer 121 could be arranged to the strip sub-folder layer paralleled with container outer surface,
As in figure 2 it is shown, each sub-folder layer 121 is parallel to a certain outer surface 11, this outer surface 11 is inoculated
Microalgae or after fixing for microalgae being seeded on cultivation carrier, a cultivation carrier is layed on this outer surface 11.
According to the volume of the condensed fluid maintained needed for microalgae normal growth, preferentially should from be adjacent to cultivate microalgae
First sub-interlayer 121 of outer surface 11 is initially added into condensed fluid, so that accommodating the sub-folder layer of condensed fluid
Corresponding with cultivation face 11.It is understood that sub-folder layer can also is that square, shaped form, broken line
Other shape such as shape, this is not construed as limiting by the present invention.
In an embodiment of the present invention, container is made up of the material that heat conductivity is high.The material of high thermal conductivity coefficient
Mass-energy enough shortens the time needed for the transmission of warm amount, is conducive to reaching preferable temperature control effect.
In an alternative embodiment of the invention, the shape of container can be cube, taper or hemispherical
In any one.This is not construed as limiting by the present invention, as long as condensed fluid can be accommodated, has
Can cultivating microalgae or the outer surface cultivating carrier of placement microalgae.Preferably, container
Shape can be taper, and its cultivation surface is bank slope formula, and the dead angle of unglazed photograph is more conducive to
Receive luminous energy.
It is understood that container has opening.Opening can be the entrance adding condensed fluid, it is simple to add
Add condensed fluid, it is also possible to be the import and export being connected with other equipment, can add cold by other equipment
Lime set, or make condensate circulation use.This container can also be exactly open-top receptacle, and such as, container is permissible
Being uncovered cubic container, uncovered upwards place, side is used for cultivating microalgae, when adding condensed fluid,
Frond cultivation height is less than the height of inside reactor condensed fluid.
It should be noted that condensed fluid can be water, it is also possible to be other liquid.Condensed fluid enters with microalgae
Row is noncontact heat exchange, and condensed fluid can be any medium with thermal capacity and thermal conductivity, this
Bright this is not construed as limiting.
Obviously, above-described embodiment is only for clearly demonstrating example, and not to reality
Execute the restriction of mode.For those of ordinary skill in the field, at the base of described above
Can also make other changes in different forms on plinth.Here without also cannot be to all
Embodiment give exhaustive.And the obvious change thus extended out or variation are still located
Protection domain in the invention.
Claims (18)
1. a microalgae immobilization cultural method, it is characterised in that
At least one container is provided；
Cultivate on carrier afterwards described at described container outer surface inoculation microalgae or by fixing for microalgae being seeded in
Cultivation carrier is layed in described container outer surface；
Microalgae supplying nutrition liquid for described container outer surface；
Adding condensed fluid inside described container, described condensed fluid and microalgae carry out indirect heat exchange.
Microalgae immobilization cultural method the most according to claim 1, it is characterised in that
Regulate the volume of described condensed fluid, make microalgae maintain the temperature range needed for normal growth.
Microalgae immobilization cultural method the most according to claim 2, it is characterised in that control institute
State the volume described condensation more than or equal to the temperature range needed for microalgae is maintained normal growth of container
The volume of liquid；
Microalgae is inoculated at described container outer surface corresponding with described condensed fluid position, or microalgae is solid
Surely after being seeded on cultivation carrier, described cultivation carrier is layed in described container outer surface.
Microalgae immobilization cultural method the most according to claim 3, it is characterised in that
The volume of the described container of described control is more than or equal to microalgae maintains the temperature needed for normal growth
The volume of the described condensed fluid of scope, particularly as follows:
According to needed for the microalgae normal growth that described condensed fluid and microalgae noncontact heat exchange are to be reached
Temperature range, it is thus achieved that the minimum volume of described condensed fluid, arranges the volume of described container more than or equal to by micro-
Algae maintain normal growth needed for the volume of described condensed fluid of temperature range.
Microalgae immobilization cultural method the most according to claim 2, it is characterised in that
The start volume arranging described container is the first volume,
According to the change of optical radiation energy, change the volume of described container.
Microalgae immobilization cultural method the most according to claim 5, it is characterised in that
Determine average of the whole year light radiation energy；
According to average of the whole year light radiation energy, determine that the volume of described container is the first volume V1；
When light radiation energy is higher than average of the whole year light radiation energy, the volume arranging described container is big
In described first volume V1；
When light radiation energy is less than equal to average of the whole year light radiation energy,
The volume of described container is set equal to described first volume V1；Or
The light radiation energy arrived according to described condensed fluid actual received and average of the whole year light radiation energy
The difference of amount draws the described condensed fluid volume of required reduction, arranges the volume of described container less than described the
One volume V1。
Microalgae immobilization cultural method the most according to claim 5, it is characterised in that
Determine the emittance of first day；
According to the emittance of first day, determine that the volume of the described container of first day is the first volume V2；
When the emittance of second day is higher than the emittance of first day, the volume arranging described container is big
In described first volume V2；
When the emittance of second day is less than the emittance equal to first day,
The volume of described container is set equal to described first volume V2；Or,
Difference according to emittance draws the described condensed fluid volume of required reduction, arranges described container
Volume is less than described first volume V2。
Microalgae immobilization cultural method the most according to claim 2, it is characterised in that
At the internal sandwich establishment of described container, described interlayer is divided into several sub-folder layers, according to described son
The quantity of interlayer controls the volume of described condensed fluid, so that microalgae to maintain the temperature model needed for normal growth
Enclose；
Microalgae is being inoculated with the corresponding described container outer surface of described sub-folder layer accommodating described condensed fluid,
Or after fixing for microalgae being seeded on cultivation carrier, described cultivation carrier is layed in described container outer surface.
Microalgae immobilization cultural method the most according to claim 1, it is characterised in that regulation institute
State the temperature of condensed fluid, make microalgae maintain the temperature range needed for normal growth.
Microalgae immobilization cultural method the most according to claim 9, it is characterised in that
Arranging temperature control device in described container, described temperature control device controls the temperature of described condensed fluid.
11. microalgae immobilization cultural methods according to claim 9, it is characterised in that
Maintain the temperature needed for normal growth according to microalgae, change the described condensed fluid in described container.
12. microalgae immobilization cultural methods according to claim 11, it is characterised in that
Arranging temperature control device outside described container, between described container and described temperature control device, circulation is described
Condensed fluid, described temperature control device regulates the temperature of described condensed fluid.
13. according to the microalgae immobilization cultural method described in claim 10 or 12, it is characterised in that
Described condensate temperature is measured and obtains feedback by described temperature control device；
Minimum temperature T needed for microalgae normal growth is provided1With maximum temperature T2；
When described condensate temperature is close to and above minimum temperature T1Time, described temperature control device starts heating
Program raises the temperature of described condensed fluid；
When described condensate temperature is close and is less than maximum temperature T2Time, described temperature control device starts refrigeration
Program reduces the temperature of described condensed fluid.
14. according to the microalgae immobilization cultural method described in claim 10 or 12, it is characterised in that
Described condensate temperature is measured and obtains feedback by described temperature control device,
Offer has fixed temperature T3Described condensed fluid, described fixed temperature T3It is in microalgae just maintaining
It is frequently grown within the scope of required temperature,
When described condensate temperature is less than described fixed temperature T3Time, described temperature control device starts heating journey
Sequence makes described condensate temperature raise；
When described condensate temperature is higher than described fixed temperature T3Time, described temperature control device starts refrigeration journey
Sequence makes described condensate temperature reduce.
15. 1 kinds of microalgae immobilization cultivation apparatus, it is characterised in that including:
At least one container；
Described container outer surface is directly distributed microalgae or cultivation carrier is distributed, and described cultivation carrier divides
It is furnished with microalgae；
Condensed fluid is had inside described container.
16. microalgae immobilization cultivation apparatus according to claim 15, it is characterised in that described
It is provided with interlayer inside container, and described interlayer comprises sub-folder layer.
17. microalgae immobilization cultivation apparatus according to claim 15, it is characterised in that described
Container is made up of the material that heat conductivity is high.
18. microalgae immobilization cultivation apparatus according to claim 15, it is characterised in that described
Container be shaped as cube, taper or hemispherical.
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| WO2011017171A1 (en) * | 2009-07-28 | 2011-02-10 | Joule Unlimited, Inc. | Photobioreactors, solar energy gathering systems, and thermal control methods |
| CN103184142A (en) * | 2011-12-29 | 2013-07-03 | 新奥科技发展有限公司 | Light biological reaction equipment and light biological culture method |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| WO2011017171A1 (en) * | 2009-07-28 | 2011-02-10 | Joule Unlimited, Inc. | Photobioreactors, solar energy gathering systems, and thermal control methods |
| CN103184142A (en) * | 2011-12-29 | 2013-07-03 | 新奥科技发展有限公司 | Light biological reaction equipment and light biological culture method |
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