CN104560634B - Immobilized microalgae breeding method and device thereof - Google Patents
Immobilized microalgae breeding method and device thereof Download PDFInfo
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- 239000011229 interlayer Substances 0.000 claims description 52
- 238000005286 illumination Methods 0.000 claims description 34
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- 239000007788 liquid Substances 0.000 claims description 12
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- SYHGEUNFJIGTRX-UHFFFAOYSA-N methylenedioxypyrovalerone Chemical compound C=1C=C2OCOC2=CC=1C(=O)C(CCC)N1CCCC1 SYHGEUNFJIGTRX-UHFFFAOYSA-N 0.000 claims description 4
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- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
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- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/12—Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature
- C12M41/18—Heat exchange systems, e.g. heat jackets or outer envelopes
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Abstract
The invention provides an immobilized microalgae breeding method and a device thereof, belongs to the field of microalgae breeding and can be used for effectively controlling the temperature in the microalgae breeding process so as to facilitate the normal growth of microalgae. The immobilized microalgae breeding method comprises the following steps: providing at least one container; inoculating microalgae on the surface of the container or fixedly inoculating the microalgae on a breeding carrier and then paving the breeding carrier on the outer surface of the container; supplying nutrient solution to the microalgae on the outer surface of the container; adding condensate in the container and carrying out indirect heat exchange on the condensate and the microalgae. The immobilized microalgae breeding method and the device thereof can be applied to the immobilized breeding of microalgae.
Description
Technical Field
The invention relates to the field of microalgae cultivation, in particular to a microalgae immobilized cultivation method and a device thereof.
Background
The microalgae immobilized culture technology is a culture mode that free microalgae cells are fixed or embedded on a carrier to enable the cells to be in a relatively static state, and culture liquid has a relatively flowing state, and has the advantages of low recovery cost, high light conversion efficiency and the like compared with the traditional microalgae culture. However, light not only provides the photosynthetic energy required for growth of the microalgae, but also raises the temperature of the microalgae. Although the immobilized culture improves the light conversion efficiency, the temperature in the microalgae culture process is greatly increased and even exceeds the temperature which can be tolerated by the normal growth of the microalgae, so that the temperature needs to be controlled to a certain extent in the microalgae culture process.
In the existing microalgae immobilization culture, condensate is generally directly applied to a culture device by spraying and the like to achieve the purpose of cooling, but the condensate is directly contacted with microalgae in the cooling process, if the proper temperature for the growth of the microalgae is to be maintained, a large liquid flow is generally required to be maintained, microalgae cells attached to a carrier are easily lost and are not beneficial to immobilization culture, and if a small liquid flow is maintained, the temperature of the condensate is required to be very low in order to maintain the proper temperature for the growth of the microalgae, and the low-temperature damage is caused to the microalgae cells attached to the carrier, so that the growth of the microalgae is inhibited.
Therefore, it is an important subject for those skilled in the art to provide a microalgae immobilized cultivation device capable of effectively controlling the temperature of microalgae and ensuring the normal growth of microalgae.
Disclosure of Invention
The embodiment of the invention provides a microalgae immobilized culture method and device, which can effectively control the temperature in the microalgae culture process and is beneficial to the normal growth of microalgae.
In order to achieve the above purpose, the embodiment of the invention adopts the following technical scheme:
the invention provides a microalgae immobilized breeding method, which comprises the following steps:
providing at least one container;
inoculating microalgae on the outer surface of the container or fixedly inoculating the microalgae on a culture carrier and then paving the culture carrier on the outer surface of the container;
supplying nutrient solution to the microalgae on the outer surface of the container;
and adding a condensate into the container, and performing indirect heat exchange between the condensate and the microalgae.
Wherein the volume of the condensate is adjusted to maintain the microalgae in a temperature range required for normal growth.
Specifically, the volume of the control container is more than or equal to the volume of condensate liquid required for maintaining the microalgae in a temperature range required by normal growth;
inoculating microalgae on the outer surface of the container corresponding to the position of the condensate, or fixedly inoculating the microalgae on a culture carrier and then paving the culture carrier on the outer surface of the container.
Preferably, the minimum volume of the condensate is obtained according to the temperature range required by the normal growth of the microalgae to be achieved by the non-contact heat exchange of the condensate and the microalgae, and the volume of the container is set to be more than or equal to the volume of the condensate for maintaining the temperature range required by the normal growth of the microalgae.
Optionally, in the above method, the method may specifically include:
the starting volume of the container is set to a first volume,
the volume of the container is changed according to the change of the light radiation energy.
Preferably, for the variation according to the energy of the light radiation, the volume of the container is varied, in particular:
determining annual average illumination radiation energy;
determining the volume of the container as a first volume V according to the annual average illumination radiation energy1；
When the illumination radiation energy is higher than the annual average illumination radiation energy, setting the volume of the container to be larger than the first volume V1；
If the illumination radiation energy is less than or equal to the annual average illumination radiation energy, setting the volume of the container to be equal to the first volume V1(ii) a Or
Obtaining the volume of the condensate to be reduced according to the difference value between the actually received illumination radiation energy of the condensate and the annual average illumination radiation energy, and setting the volume of the container to be smaller than the first volume V1。
Preferably, for varying the volume of the container according to the variation of the energy of the light radiation, it is also possible to:
determining a radiant energy of a first day;
determining the volume of the container of the first day as a first volume V according to the radiation energy of the first day2；
If the radiant energy of the second day is higher than that of the first day, setting the volume of the container to be larger than the first volume V2；
Setting the volume of the container equal to the first volume if the radiation energy of the second day is lower than the radiation energy of the first dayProduct V2Or, depending on the difference in the radiation energies, the required reduced volume of condensate is obtained, the volume of the container being set smaller than the first volume V2。
Optionally, an interlayer is arranged in the container, the interlayer is divided into a plurality of sub-interlayers, and the volume of the condensate is controlled according to the number of the sub-interlayers, so that the microalgae can be maintained in a temperature range required by normal growth;
inoculating microalgae on the outer surface of the container corresponding to the sub-interlayer containing the condensate, or fixedly inoculating the microalgae on a culture carrier and then paving the culture carrier on the outer surface of the container.
Optionally, the temperature of the condensate is adjusted to maintain the microalgae in the temperature range required for normal growth.
In particular, a temperature control device is provided within the vessel, which controls the temperature of the condensate.
Optionally, the condensate in the vessel is replaced according to the temperature required for the microalgae to maintain normal growth.
In particular, a temperature control device is provided outside the container, between which the condensate is circulated, which temperature control device regulates the temperature of the condensate.
Preferably, the temperature of the condensate is adjusted by a temperature control device, which may be:
the temperature control equipment measures the temperature of the condensate and obtains feedback;
providing the minimum temperature T required by the normal growth of the microalgae1And maximum temperature T2；
When the condensate temperature is close to and higher than the minimum temperature T1When the condensate is cooled, the temperature control equipment starts a heating program to raise the temperature of the condensate;
when the temperature of the condensate approaches and is lower than the maximum temperature T2And when the temperature control equipment starts a refrigeration program, the temperature of the condensate is reduced.
Preferably, the temperature of the condensate is regulated by a temperature control device, which may also be:
the temperature control device measures the temperature of the condensate and obtains feedback,
providing a fixed temperature T3Of the condensate of (1), fixed temperature T3Within the temperature range required by the microalgae to maintain normal growth,
when the temperature of the condensate is lower than the fixed temperature T3When the temperature control equipment is used, starting a heating program to raise the temperature of the condensate;
when the temperature of the condensate is higher than the fixed temperature T3And when the temperature control equipment starts a refrigeration program, the temperature of the condensate is reduced.
Correspondingly, the invention also provides a microalgae immobilized culture device, which comprises:
at least one container;
microalgae are directly distributed on the outer surface of the container or a culture carrier is distributed on the outer surface of the container, and the microalgae are distributed on the culture carrier;
the interior of the container is provided with condensate.
Wherein, the container is internally provided with an interlayer, and the interlayer comprises a sub-interlayer.
Preferably, the container is made of a material having a high thermal conductivity.
Optionally, the container is in the shape of a cube, cone or hemisphere.
The embodiment of the invention provides a microalgae immobilized culture method, wherein condensate is added in a container, and the temperature of microalgae can be maintained in a temperature range required by normal growth of the microalgae through indirect heat exchange between the condensate and the microalgae, so that the aim of controlling the culture temperature of the microalgae is fulfilled.
Drawings
FIG. 1 is a schematic flow chart of a microalgae immobilization culture method provided in an embodiment of the invention;
fig. 2 is a schematic structural diagram of a microalgae immobilized cultivation device provided in an embodiment of the invention.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Embodiments of the present invention will be described in detail below with reference to the accompanying drawings.
As shown in fig. 1, an embodiment of the present invention provides a microalgae immobilized cultivation method, which specifically includes:
s1, providing at least one container;
s2, inoculating microalgae on the outer surface of the container or fixedly inoculating the microalgae on a culture carrier and then paving the culture carrier on the outer surface of the container;
s3, supplying nutrient solution to the microalgae on the outer surface of the container;
and S4, adding a condensate into the container, and performing indirect heat exchange between the condensate and the microalgae.
The embodiment of the invention provides a microalgae immobilized culture method, wherein condensate is added in a container, and the temperature of microalgae can be maintained in a temperature range required by normal growth of the microalgae through indirect heat exchange between the condensate and the microalgae, so that the aim of controlling the culture temperature of the microalgae is fulfilled.
In one embodiment of the invention, the volume of the condensate is adjusted to maintain the temperature range required for normal growth of the microalgae.
The heat transfer effect between the condensate and the algae is utilized to increase the temperature of the condensate and simultaneously reduce the temperature of the algae, and finally, the condensate and the algae reach an equilibrium temperature. In the case of a determined specific heat capacity of the condensate, the volume of condensate required to reach the equilibrium temperature is determined. The purpose of controlling the temperature of the cultured algae can be achieved by adjusting the volume of the condensate, so that the balance temperature is not higher than the highest temperature which can be tolerated by the growth of the microalgae and not lower than the lowest temperature which can be tolerated by the growth of the microalgae.
In another embodiment of the invention, the volume of the control vessel is greater than or equal to the volume of condensate required to maintain the microalgae in the temperature range required for normal growth; inoculating microalgae on the outer surface of the container corresponding to the position of the condensate, or fixedly inoculating the microalgae on a culture carrier and then paving the culture carrier on the outer surface of the container.
The volume of the condensate can be adjusted by adjusting the volume of the vessel. Typically, the volume of the vessel is fixed and the volume of the condensate is the volume of the vessel, i.e. how much volume of the vessel is required to maintain the microalgae in the temperature range required for normal growth, the volume of the vessel can be set, at which point the condensate fills the entire vessel, the condensate corresponding to the entire outer surface of the vessel.
The volume of the container is controlled to be larger than the volume of the condensate which is required for maintaining the microalgae in the temperature range required for normal growth, and the volume of the container has a certain margin space relative to the volume of the condensate which is required for maintaining the microalgae in the proper temperature, which is equivalent to increasing the volume of the condensate which can be added into the container, so that the volume of the condensate is not limited to the volume of the container, and if the temperature of the microalgae is abnormally increased due to abnormal conditions in the culture process, the volume of the condensate which is actually added can be adjusted to deal with the abnormal conditions. At the moment, the microalgae is inoculated on the outer surface of the container corresponding to the position of the condensate, or the culture carrier is laid on the outer surface of the container after the microalgae is fixedly inoculated on the culture carrier, so that the indirect heat exchange between the condensate and the microalgae is facilitated.
In another embodiment of the present invention, the minimum volume of the condensate is obtained according to the temperature range required for the normal growth of the microalgae to be achieved by indirect heat exchange of the condensate with the microalgae, and the volume of the container is set to be equal to or greater than the volume of the condensate required for maintaining the microalgae in the temperature range required for the normal growth.
The specific setting method is as follows:
setting the difference between the equilibrium temperature reached by heat exchange between the condensate and the microalgae and the initial temperature of the condensate as delta T, setting the illumination radiation energy required to be absorbed by the condensate as delta Q for ensuring that the temperature of the culture system is maintained at the equilibrium temperature, and setting the highest light radiation energy capable of being received by the ground in a single day in the culture season of a culture area as Q1Setting the energy corresponding to the highest temperature which can be tolerated by the growth of the microalgae as Q2The volume of the condensate in the vessel is set to VminThe density of the condensate is rho, the specific heat capacity of the condensate is c, the equilibrium temperature is lower than the highest temperature which can be endured by the growth of the microalgae,
then the respective values at, at Q, Q1、Q2、VminRho, c should satisfy the following relationship,
Vmin＝ΔQ/(c·ΔT·ρ)＝(Q1-Q2)/(c·ΔT·ρ)。
wherein, VminI.e. the minimum volume of condensate required to maintain the microalgae in the temperature range required for normal growth, which is also the minimum volume of the container, wherein the volume of the container is set to be greater than or equal to VminThe container can be ensured to contain enough condensate so as to ensure that the equilibrium temperature of the condensate after heat exchange with the microalgae is not higher than the highest temperature and not lower than the lowest temperature which can be tolerated by the growth of the microalgae.
It should be noted that the temperature required for maintaining normal growth of microalgae has the lowest value and the highest value, and accordingly, the volume of condensate required for maintaining normal growth of microalgae also has the lowest value and the highest value, and therefore, when the volume of the container is set to be equal to or greater than the volume of condensate in the temperature range required for maintaining normal growth of microalgae, the volume of the container is not increased without limitation.
In another embodiment of the present invention, the volume of the container may be adjusted according to the actual conditions of the cultivation process, which may specifically include:
setting a starting volume of the container to a first volume; the volume of the container is changed according to the change of the light radiation energy.
Under the condition that the specific heat capacity, the density and the like of the condensate are determined, the volume change of the condensate is obtained according to the corresponding relation between the light radiation energy and the volume of the condensate, and then the volume change of the container is obtained according to the volume of the condensate, so that the volume of the container is adjusted, for example, the difference between the equilibrium temperature of the condensate and microalgae achieved through heat exchange and the initial temperature of the condensate is set to be delta T, and when the light radiation energy is changed, the temperature difference delta T is changed according to delta V (Q)1-Q2) And/(+/-. DELTA.T.. rho), the volume of the container can be a first volume +/-DELTA.V, i.e., the volume of the container can be changed accordingly, so that the volume of the condensate can be changed, and the heat transfer between the condensate and the microalgae can be adjusted, so that the microalgae can be maintained at a proper temperature.
The volume of the container is adjusted according to the change of the light radiation energy, so that the utilization rate of the container to the culture floor area can be effectively adjusted in a unit culture area, and the number of the containers in the culture system can be flexibly adjusted.
In a preferred embodiment of the present invention, the volume of the container can be changed according to the change of the light radiation energy during the annual cultivation process, and the change of the light radiation energy can specifically include:
determining annual average illumination radiation energy;
determining the volume of the container as a first volume V according to the annual average illumination radiation energy1；
When the irradiation energy is higher than the annual average irradiation energy, the volume of the container may be set to be larger than the first volume V1；
The volume of the container may be set equal to the first volume V when the illumination radiation energy is less than or equal to the annual average illumination radiation energy1(ii) a Or
The volume of the condensate to be reduced can be obtained according to the difference value between the actually received illumination radiation energy of the condensate and the annual average illumination radiation energy, and the volume of the container is set to be smaller than the first volume V1。
In the whole year cultivation process, the illumination radiation energy changes along with the change of seasons and months, and the illumination radiation energy actually received by the condensate changes along with the change of the seasons and months. In consideration of the change, the volume of the container can be correspondingly adjusted to ensure that the volume of the container is matched with the volume of the condensate, so that the container can contain the condensate with proper volume, and the aim of controlling the temperature in the process of microalgae culture is fulfilled.
The specific adjustment process may be:
setting the difference between the equilibrium temperature of the condensate and the microalgae reached through heat exchange and the initial temperature of the condensate as delta T, setting the illumination radiation energy required to be absorbed by the condensate as delta Q for ensuring that the temperature of the culture system is maintained at the equilibrium temperature, and setting the highest light radiation energy capable of being received by the ground in a single day in the culture season of a culture area as Q1Setting the energy corresponding to the highest temperature which can be tolerated by the growth of the microalgae as Q2The volume of the condensate in the container is Vmin, the density of the condensate is rho, the specific heat capacity of the condensate is c, the equilibrium temperature is lower than the highest temperature which can be endured by the growth of microalgae,
then the respective values at, at Q, Q1、Q2Vmin, ρ, c should satisfy the following relationship,
Vmin＝ΔQ/(c·ΔT·ρ)＝(Q1-Q2)/(c·ΔT·ρ)；
setting the difference between the equilibrium temperature of the condensate and the microalgae after heat exchange and the initial temperature of the condensate as delta T, and setting the average illumination radiation energy required to be absorbed in the culture season of the condensate as delta Q to ensure that the temperature of the culture system is maintained at the equilibrium temperatureAverageThe average light radiation energy which can be received by the ground in a single day in the breeding season of the breeding area is set as Q3Setting the energy corresponding to the highest temperature which can be tolerated by the growth of the microalgae as Q2The volume of the condensate in the housing is set to VAverageThe density of the condensate is rho, the specific heat capacity of the condensate is c, the equilibrium temperature is lower than the highest temperature which can be endured by the growth of the microalgae,
then the respective values Δ T, Δ QAverage、Q3、Q2、VAverageRho, c should satisfy the following relationship,
Vaverage＝ΔQAverage/(c·ΔT·ρ)＝(Q3-Q2)/(c·ΔT·ρ)；
When the irradiation energy is higher than the annual average irradiation energy, the volume of the container may be set to be larger than the first volume V1Preferably, the volume of the condensate to be increased is obtained according to the difference between the actually received illumination radiation energy of the condensate and the annual average illumination radiation energy, and the volume of the container is set to be V1+ΔV；
When the illumination radiation energy is lower than or equal to the annual average illumination radiation energy, the volume of the container can be not adjusted, namely the volume of the container is equal to the first volume V1(ii) a Or
Obtaining the volume of the condensate to be reduced according to the difference value between the actually received illumination radiation energy of the condensate and the annual average illumination radiation energy, and setting the volume of the container to be smaller than the first volume V1Preferably, the volume of the container is setIs a V1-ΔV。
In another preferred embodiment of the present invention, the volume of the container can be changed according to the change of the light radiation energy in the continuous cultivation process, and the method specifically comprises the following steps:
determining a radiant energy of a first day;
determining the volume of the container of the first day as a first volume V according to the radiation energy of the first day2；
If the radiant energy of the second day is higher than that of the first day, setting the volume of the container to be larger than the first volume V2；
If the radiation energy of the next day is lower than or equal to the radiation energy of the first day, setting the volume of the container to be equal to the first volume, or obtaining the volume of the condensate needing to be reduced according to the difference of the radiation energy, and setting the volume of the container to be smaller than the first volume V2。
That is, the difference Δ V of the volume of the required condensate is obtained according to the difference Δ Q of the radiation energy, i.e. the difference Δ V of the volume of the container to be adjusted, and if the radiation energy of the second day is higher than the radiation energy of the first day, the volume of the container is set to be larger than the first volume V2Preferably, the required increased volume of condensate can be derived from the difference in radiant energy, and the volume of the vessel is set at V2+ Δ V; if the radiation energy of the second day is lower than the radiation energy of the first day, the volume of the container can be set to V2I.e. keeping the volume of the container constant, or obtaining the required reduced volume of condensate from the difference in radiant energy, setting the volume of the container to V2-ΔV。
In another embodiment of the invention, an interlayer can be arranged in the container, the interlayer is divided into a plurality of sub-interlayers, and the volume of the condensate is controlled according to the number of the sub-interlayers, so that the microalgae can be maintained in a temperature range required by normal growth; inoculating microalgae on the outer surface of the container corresponding to the sub-interlayer containing the condensate, or fixedly inoculating the microalgae on a culture carrier and then paving the culture carrier on the outer surface of the container.
It will be appreciated that the interlayer may be a divided grid, i.e. the interlayer is divided into a plurality of sub-interlayers in a grid. The volume of each sub-sandwich may or may not be equal.
Through set up the intermediate layer in container inside, will the intermediate layer divide into a plurality of sub-intermediate layers, control the volume of condensate according to the quantity of sub-intermediate layer to keep little algae in the required temperature range of normal growth, specifically include:
firstly, determining the volume of condensate required for maintaining the microalgae in a temperature range required by normal growth; setting the total volume of the interlayer to be larger than the volume of the condensate, and equally dividing or gradiently dividing the interlayer into a plurality of sub-interlayers; determining the number of the required sub-interlayers according to the volume of the condensate, namely adding the condensate into the sub-interlayers in the interlayers; in this way, the volume of condensate can be controlled by controlling the number of sub-layers. In order to obtain better cultivation effect, the sub-interlayer containing the condensate is adjacent and parallel to one outer surface of the container, and the condensate is filled in the sub-interlayer adjacent to the outer surface preferentially, namely, the microalgae is inoculated on the outer surface corresponding to the sub-interlayer added with the condensate, or the cultivation carrier is laid on the outer surface after the microalgae is fixedly inoculated on the cultivation carrier.
It should be noted that the outer surface inoculated with the microalgae or the outer surface on which the cultivation carrier is laid after the microalgae is fixedly inoculated on the cultivation carrier can be regarded as the cultivation surface, and in order to better maintain the microalgae in the temperature range required by normal growth, no matter several sub-interlayers are required to be arranged in the interlayer, the sub-interlayer corresponding to the cultivation surface must be filled with condensate. Specifically, in order to ensure that the sub-interlayers corresponding to the culture surfaces are filled with the condensate, when a plurality of sub-interlayers need to be arranged in the interlayer, the condensate is sequentially filled into the next adjacent sub-interlayer from the sub-interlayer corresponding to the culture surfaces.
Alternatively, the volume of each sub-sandwich may be set according to the optical radiation energy variation. For example, if the difference in the variation of the light radiation energy is Δ Q during the cultivation process, the volume of each sub-sandwich may be set to V ═ Δ Q/(c · Δ T · ρ), specifically, taking as an example during the continuous cultivation process:
when the light radiation energy of the next day is higher than that of the previous day, the number of the sub-interlayers can be increased; when the light radiation energy of the next day is lower than that of the previous day, the number of the sub-interlayers can be reduced, so that the volume of the condensate can be adjusted according to the adjustment of the number of the interlayers containing the condensate, the temperature range required by normal growth of the microalgae can be maintained, meanwhile, the culture surface can be ensured to correspond to the sub-interlayers containing the condensate, a better heat transfer effect can be obtained, and a better culture effect can be obtained.
In yet another embodiment of the invention, the temperature of the condensate is adjusted to maintain the microalgae in the temperature range required for normal growth.
When the temperature of the condensate is adjusted, the volume of the condensate can be kept fixed under the normal condition, so that the temperature of the condensate can be adjusted according to T ═ delta Q/(c · V · ρ) (Q1-Q2)/(c · V · ρ) under the condition that the specific heat capacity and the density of the condensate are determined, and the heat exchange between the condensate and the microalgae can be ensured to ensure that the microalgae can maintain the normal required temperature.
In a preferred embodiment of the invention, a temperature control device is provided within the vessel, which temperature control device controls the temperature of the condensate.
Through set up temperature control equipment in the container, directly carry out temperature control to the inside condensate of container, be favorable to the temperature of the inside condensate of more direct, more quick control container to shorten the condensate and carry out the heat exchange with little algae and reach balanced time, and then be favorable to realizing the control to little algae temperature fast. The temperature of the condensate is adjusted by the temperature control device, and two ways can be specifically included,
the first method is as follows:
the temperature control equipment measures the temperature of the condensate and obtains feedback;
providing the minimum temperature T required by the normal growth of the microalgae1And maximum temperature T2；
When the condensate temperature is close to and higher than T1When the condensate is cooled, the temperature control equipment starts a heating program to raise the temperature of the condensate;
when the temperature of the condensate approaches and is lower than T2And when the temperature control equipment starts a refrigeration program, the temperature of the condensate is reduced.
The suitable temperature for maintaining the normal growth of the microalgae has a certain range, and the lower limit value is the lowest temperature T1The upper limit value is the maximum temperature T2When the condensate temperature is close to and above T1When the temperature of the condensate is close to the lower limit value and still between the lower limit value and the upper limit value, if the temperature can be set to exceed the lower limit value by 0.1, 0.2, 0.5, 0.8, 1, 2, 3 ℃ and the like, the temperature control device starts a heating program to increase the temperature of the condensate; when the temperature of the condensate approaches and is lower than T2When the temperature of the condensate is close to the lower limit value and still between the upper limit value and the lower limit value, for example, the temperature can be set to be lower than the upper limit value by 0.1, 0.2, 0.5, 0.8, 1, 2, 3 ℃ and the like, and at this time, the temperature control device starts a refrigeration program to reduce the temperature of the condensate. Thus, the temperature of the condensate can be ensured to be always kept within the proper temperature range required by the microalgae.
The second method comprises the following steps:
the temperature control equipment measures the temperature of the condensate and obtains feedback;
providing a fixed temperature T3The fixed temperature of the condensate is in the temperature range required by the normal growth of the microalgae;
when the temperature of the condensate is lower than T3When the temperature control equipment is used, starting a heating program to raise the temperature of the condensate;
when the condensate temperature is higher than T3And when the temperature control equipment starts a refrigeration program, the temperature of the condensate is reduced.
The temperature is fixed at a preset temperature, the preset temperature is a certain value in a temperature range required by the normal growth of the microalgae, can be an optimal value required by the normal growth of the microalgae, and can also be an intermediate value.
In another embodiment of the invention, the condensate in the vessel is replaced according to the temperature required for the microalgae to maintain normal growth. Specifically, the temperature of the condensate is measured, and if the temperature of the condensate is higher than the temperature required for maintaining normal growth of the microalgae, the condensate is discharged and replaced by a new condensate with a lower temperature; if the temperature of the condensate is lower than the temperature required to maintain normal growth, the condensate is discharged and replaced with new condensate having a higher temperature.
In a preferred embodiment of the invention, a temperature control device is arranged outside the container, the condensate being circulated between the container and the temperature control device, the temperature control device regulating the temperature of the condensate.
Likewise, the temperature of the condensate is regulated by the temperature control device, and two ways can be specifically included:
the temperature control device measures and feeds back the temperature of the condensate, 1) the lowest temperature T1And maximum temperature T2Starting temperature control equipment to regulate the temperature of the condensate as a reference; or 2) starting the temperature control device to regulate the temperature of the condensate based on the preset stockholder temperature.
It will be appreciated that the operation of the temperature control device may be manual or automated on-line.
In addition, the type, the precision and the like of the temperature control equipment are not limited in the invention, as long as the temperature of the condensate liquid can be regulated, the embodiment of the invention is not limited to the above, and the skilled person can select or determine the temperature according to the disclosure of the invention and common general knowledge or common technical means in the field.
Correspondingly, the invention also provides a microalgae immobilized cultivation device, as shown in fig. 2, comprising:
at least one container 10;
microalgae are directly distributed on the outer surface 11 of the container or a culture carrier is distributed on the outer surface of the container, and the microalgae are distributed on the culture carrier;
the interior of the container is provided with condensate.
The embodiment of the invention provides a microalgae immobilized culture device, wherein condensate is added in a container, and the temperature of microalgae can be maintained in a temperature range required by normal growth of the microalgae through indirect heat exchange between the condensate and the microalgae, so that the aim of controlling the culture temperature of the microalgae is fulfilled.
As shown in fig. 2, the interlayer 12 is disposed inside the container 10, the interlayer 12 is divided into a plurality of sub-interlayers 121, the sub-interlayers may be in a separated grid shape, that is, the interlayer 12 is divided into a plurality of sub-interlayers 121 in a grid shape, and the volumes of the sub-interlayers 121 may be equal or different.
The grid-like sub-layers 121 may be arranged as strip-like sub-layers parallel to the outer surface of the container, as shown in fig. 2, each sub-layer 121 is parallel to one outer surface 11, and the outer surface 11 is inoculated with microalgae or the microalgae is immobilized on a culture carrier, and then the culture carrier is laid on the outer surface 11. Depending on the volume of condensate required to maintain normal growth of the microalgae, the condensate is preferably added from the first sub-sandwich 121 adjacent to the outer surface 11 where the microalgae are cultivated, so that the sub-sandwich containing the condensate corresponds to the cultivation surface 11. It will be appreciated that the sub-sandwich may have other shapes such as square, curved, dog-leg, etc., and the present invention is not limited thereto.
In one embodiment of the present invention, the container is made of a material with a high thermal conductivity. The material with high heat conductivity coefficient can shorten the time required by heat transfer, and is favorable for achieving a better temperature control effect.
In another embodiment of the present invention, the shape of the container may be any one of a cubic shape, a conical shape, or a hemispherical shape. The present invention is not limited to this, and may be any type of culture medium that can contain condensate and has an outer surface on which microalgae can be cultured or on which microalgae can be placed. Preferably, the container can be in a conical shape, the culture surface of the container is in a plane slope type, no dead angle of illumination exists, and light energy can be received more favorably.
It will be appreciated that the container has an opening. The opening can be an inlet for adding the condensate, so that the condensate can be conveniently added, and can also be an inlet and an outlet which are connected with other equipment, so that the condensate can be added by the other equipment, or the condensate can be recycled. The container can be an open container, for example, the container can be an open cubic container, the opening is placed upwards, the side surface is used for cultivating microalgae, and when the condensate is added, the height of the cultivated microalgae is lower than that of the condensate in the reactor.
The condensate may be water or other liquid. The condensate is subjected to non-contact heat exchange with the microalgae, and the condensate may be any medium having heat capacity and thermal conductivity, and the invention is not limited thereto.
It should be understood that the above examples are only for clarity of illustration and are not intended to limit the embodiments. Other variations and modifications will be apparent to persons skilled in the art in light of the above description. And are neither required nor exhaustive of all embodiments. And obvious variations or modifications can be made without departing from the scope of the invention.
Claims (18)
1. A microalgae immobilized breeding method is characterized in that,
providing at least one container;
inoculating microalgae on the outer surface of the container or fixedly inoculating the microalgae on a culture carrier and then paving the culture carrier on the outer surface of the container;
supplying nutrient solution to the microalgae on the outer surface of the container;
and adding a condensate into the container, wherein the condensate is subjected to indirect heat exchange with the microalgae.
2. The immobilized microalgae cultivation method of claim 1,
and adjusting the volume of the condensate to maintain the microalgae in a temperature range required by normal growth.
3. The immobilized microalgae cultivation method of claim 2, wherein the volume of the container is controlled to be equal to or larger than the volume of the condensate liquid required for maintaining the microalgae in the temperature range required for normal growth;
and inoculating microalgae on the outer surface of the container corresponding to the position of the condensate, or fixedly inoculating the microalgae on a culture carrier and then paving the culture carrier on the outer surface of the container.
4. The immobilized microalgae cultivation method of claim 3,
the volume of the container is controlled to be more than or equal to the volume of the condensate for maintaining the microalgae in the temperature range required by normal growth, and the control method specifically comprises the following steps:
and obtaining the minimum volume of the condensate according to the temperature range required by the normal growth of the microalgae, which is achieved by the non-contact heat exchange of the condensate and the microalgae, and setting the volume of the container to be more than or equal to the volume of the condensate for maintaining the microalgae in the temperature range required by the normal growth.
5. The immobilized microalgae cultivation method of claim 2,
setting a starting volume of the container to a first volume,
the volume of the container is changed according to the change of the light radiation energy.
6. The immobilized microalgae cultivation method of claim 5,
determining annual average illumination radiation energy;
determining the volume of the container as a first volume V according to the annual average illumination radiation energy1；
When the illumination radiation energy is higher than the annual average illumination radiation energy, setting the volume of the container to be larger than the first volume V1；
When the illumination radiation energy is lower than or equal to the annual average illumination radiation energy,
setting the volume of the container equal to the first volume V1(ii) a Or
Obtaining the volume of the condensate required to be reduced according to the difference value between the actually received illumination radiation energy of the condensate and the annual average illumination radiation energy, and setting the volume of the container to be smaller than the first volume V1。
7. The immobilized microalgae cultivation method of claim 5,
determining a radiant energy of a first day;
determining the volume of the container on the first day as a first volume V according to the radiation energy on the first day2；
Setting the volume of the container to be larger than the first volume V when the radiant energy of the second day is higher than that of the first day2；
When the radiation energy of the second day is lower than the radiation energy of the first day,
setting the volume of the container equal to the first volume V2(ii) a Or,
the volume of the condensate liquid required to be reduced is obtained according to the difference of the radiation energy, and the volume of the container is set to be smaller than the first volume V2。
8. The immobilized microalgae cultivation method of claim 2,
arranging an interlayer in the container, dividing the interlayer into a plurality of sub-interlayers, and controlling the volume of the condensate according to the number of the sub-interlayers so as to maintain the microalgae in a temperature range required by normal growth;
and inoculating microalgae on the outer surface of the container corresponding to the sub-interlayer containing the condensate, or fixedly inoculating the microalgae on a culture carrier and then paving the culture carrier on the outer surface of the container.
9. The immobilized microalgae cultivation method of claim 1, wherein the temperature of the condensate is adjusted to maintain the microalgae in the temperature range required for normal growth.
10. The immobilized microalgae cultivation method of claim 9,
and arranging a temperature control device in the container, wherein the temperature control device controls the temperature of the condensate.
11. The immobilized microalgae cultivation method of claim 9,
and replacing the condensate in the container according to the temperature required by the normal growth of the microalgae.
12. The immobilized microalgae cultivation method of claim 11,
a temperature control device is provided outside the container, the condensate is circulated between the container and the temperature control device, and the temperature control device adjusts the temperature of the condensate.
13. The immobilized microalgae cultivation method according to claim 10 or 12,
the temperature control equipment measures the temperature of the condensate and obtains feedback;
providing the minimum temperature T required by the normal growth of the microalgae1And maximum temperature T2；
When the temperature of the condensed liquid is close to and higher than the minimum temperature T1When the temperature control device starts a heating program to lift the condensateThe temperature of (a);
when the temperature of the condensed liquid is close to and lower than the maximum temperature T2And starting a refrigeration program by the temperature control equipment to reduce the temperature of the condensate.
14. The immobilized microalgae cultivation method according to claim 10 or 12,
the temperature control device measures the temperature of the condensate and obtains feedback,
providing a constant temperature T3The fixed temperature T of the condensate3Within the temperature range required by the microalgae to maintain normal growth,
when the temperature of the condensed liquid is lower than the fixed temperature T3When the temperature control equipment starts a heating program, the temperature of the condensate is increased;
when the condensate temperature is higher than the fixed temperature T3And starting a refrigeration program by the temperature control equipment to reduce the temperature of the condensed liquid.
15. A microalgae immobilized culture device is characterized by comprising:
at least one container;
microalgae are directly distributed on the outer surface of the container or a culture carrier is distributed on the outer surface of the container, and the microalgae are distributed on the culture carrier;
the container has a condensate inside.
16. The immobilized microalgae cultivation device of claim 15, wherein the container is internally provided with an interlayer, and the interlayer comprises a sub-interlayer.
17. The immobilized microalgae cultivation device of claim 15, wherein the container is made of a material with high thermal conductivity.
18. The immobilized microalgae cultivation device of claim 15, wherein the container is cubic, conical or hemispherical in shape.
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| WO2011017171A1 (en) * | 2009-07-28 | 2011-02-10 | Joule Unlimited, Inc. | Photobioreactors, solar energy gathering systems, and thermal control methods |
| CN103184142A (en) * | 2011-12-29 | 2013-07-03 | 新奥科技发展有限公司 | Light biological reaction equipment and light biological culture method |
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| WO2011017171A1 (en) * | 2009-07-28 | 2011-02-10 | Joule Unlimited, Inc. | Photobioreactors, solar energy gathering systems, and thermal control methods |
| CN103184142A (en) * | 2011-12-29 | 2013-07-03 | 新奥科技发展有限公司 | Light biological reaction equipment and light biological culture method |
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