CN104543611A - Application of whey protein and reducing oligosaccharide Maillard product to microcapsule wall materials and embedded probiotics - Google Patents
Application of whey protein and reducing oligosaccharide Maillard product to microcapsule wall materials and embedded probiotics Download PDFInfo
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- 239000003094 microcapsule Substances 0.000 title claims abstract description 60
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- 235000018291 probiotics Nutrition 0.000 title claims abstract description 22
- 102000007544 Whey Proteins Human genes 0.000 title abstract description 3
- 108010046377 Whey Proteins Proteins 0.000 title abstract description 3
- 108010005094 Advanced Glycation End Products Proteins 0.000 title abstract 4
- 229920001542 oligosaccharide Polymers 0.000 title abstract 3
- 150000002482 oligosaccharides Chemical class 0.000 title abstract 3
- 235000021119 whey protein Nutrition 0.000 title abstract 2
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- 238000006243 chemical reaction Methods 0.000 claims abstract description 10
- 235000013305 food Nutrition 0.000 claims abstract description 7
- 235000013406 prebiotics Nutrition 0.000 claims abstract description 6
- 238000010438 heat treatment Methods 0.000 claims abstract description 5
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- 239000008267 milk Substances 0.000 claims abstract 3
- 210000004080 milk Anatomy 0.000 claims abstract 3
- 239000003814 drug Substances 0.000 claims abstract 2
- 239000000243 solution Substances 0.000 claims description 38
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- 102000004407 Lactalbumin Human genes 0.000 claims description 20
- 108090000942 Lactalbumin Proteins 0.000 claims description 20
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- KRKNYBCHXYNGOX-UHFFFAOYSA-N citric acid Chemical compound OC(=O)CC(O)(C(O)=O)CC(O)=O KRKNYBCHXYNGOX-UHFFFAOYSA-N 0.000 description 3
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- 239000011780 sodium chloride Substances 0.000 description 1
- 239000004328 sodium tetraborate Substances 0.000 description 1
- 235000010339 sodium tetraborate Nutrition 0.000 description 1
- 235000019710 soybean protein Nutrition 0.000 description 1
- 235000019698 starch Nutrition 0.000 description 1
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- 230000001954 sterilising effect Effects 0.000 description 1
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- 235000015112 vegetable and seed oil Nutrition 0.000 description 1
- 239000008158 vegetable oil Substances 0.000 description 1
- 238000005406 washing Methods 0.000 description 1
- 239000002699 waste material Substances 0.000 description 1
- DGVVWUTYPXICAM-UHFFFAOYSA-N β‐Mercaptoethanol Chemical class OCCS DGVVWUTYPXICAM-UHFFFAOYSA-N 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23C—DAIRY PRODUCTS, e.g. MILK, BUTTER OR CHEESE; MILK OR CHEESE SUBSTITUTES; MAKING THEREOF
- A23C9/00—Milk preparations; Milk powder or milk powder preparations
- A23C9/12—Fermented milk preparations; Treatment using microorganisms or enzymes
- A23C9/123—Fermented milk preparations; Treatment using microorganisms or enzymes using only microorganisms of the genus lactobacteriaceae; Yoghurt
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23C—DAIRY PRODUCTS, e.g. MILK, BUTTER OR CHEESE; MILK OR CHEESE SUBSTITUTES; MAKING THEREOF
- A23C19/00—Cheese; Cheese preparations; Making thereof
- A23C19/02—Making cheese curd
- A23C19/032—Making cheese curd characterised by the use of specific microorganisms, or enzymes of microbial origin
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23G—COCOA; COCOA PRODUCTS, e.g. CHOCOLATE; SUBSTITUTES FOR COCOA OR COCOA PRODUCTS; CONFECTIONERY; CHEWING GUM; ICE-CREAM; PREPARATION THEREOF
- A23G9/00—Frozen sweets, e.g. ice confectionery, ice-cream; Mixtures therefor
- A23G9/32—Frozen sweets, e.g. ice confectionery, ice-cream; Mixtures therefor characterised by the composition containing organic or inorganic compounds
- A23G9/36—Frozen sweets, e.g. ice confectionery, ice-cream; Mixtures therefor characterised by the composition containing organic or inorganic compounds containing microorganisms or enzymes; containing paramedical or dietetical agents, e.g. vitamins
- A23G9/363—Frozen sweets, e.g. ice confectionery, ice-cream; Mixtures therefor characterised by the composition containing organic or inorganic compounds containing microorganisms or enzymes; containing paramedical or dietetical agents, e.g. vitamins containing microorganisms, enzymes
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2400/00—Lactic or propionic acid bacteria
- A23V2400/11—Lactobacillus
- A23V2400/125—Casei
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2400/00—Lactic or propionic acid bacteria
- A23V2400/11—Lactobacillus
- A23V2400/169—Plantarum
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2400/00—Lactic or propionic acid bacteria
- A23V2400/11—Lactobacillus
- A23V2400/175—Rhamnosus
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- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Microbiology (AREA)
- Engineering & Computer Science (AREA)
- Food Science & Technology (AREA)
- Polymers & Plastics (AREA)
- Health & Medical Sciences (AREA)
- Nutrition Science (AREA)
- Inorganic Chemistry (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Medicinal Preparation (AREA)
Abstract
The invention takes protein and a reducing oligosaccharide Maillard product as microcapsule wall materials and applies the protein and the reducing oligosaccharide Maillard product to the production of probiotic microcapsules. A wall material solution is prepared by reaction of following components: the whey protein and isomaltooligosacharide are mixed according to the mass ratio ranging from (1:1) to (10:1) to form a solution, heating treatment is carried out at a constant temperature of 85-95 DEG C for 2-4 hours, Maillard reaction is carried out to generate grafts with different contents, which are used as a wall material solution. The concentrated probiotics are used as a core material, and an endogenesis emulsification gel method and a spraying and drying method are used for producing the probiotic microcapsules. The microcapsule wall materials comprise the milk-based protein and the prebiotics, have nutritional and functional properties and have a wide application range. A Maillard product is used as a wall material structure and is dense, so that the protection capability is enhanced; the fixed point releasing capability is enhanced in the presence of intestinal canal enzymes and intestinal canal microorganisms; and after the releasing, the microcapsule wall materials can provide nutrients for the probiotics. The prepared microcapsules are used as function factors, have a good market prospect and can be used for medicine, food and feed industries.
Description
Technical field
The invention belongs to microcapsules technology field, specifically albumen and reproducibility compound sugar Mei Lade product are as microcapsule wall material and the application embedding probio.
Background technology
Probio is defined as by food and agricultural commission (FAO) and world health organization (WHO) in human body intestinal canal, reaches the quasi-microorganism that some can be useful to human body.Under human body alimentary canal condition, how to protect probio to maintain vigour reaching assigned address performance prebiotic effect is the problem that food industry will solve.So how research keeps the probio of high quantity to transmit and surely grows after target location release and release being the problem that mainly will solve.
The method of current more use protects probio with microcapsules.Conventional single wall material has sodium alginate, shitosan, other polysaccharide (dextrin, cellulose derivative class, converted starch etc.); protide (gelatin, casein sodium, lactalbumin, soybean protein etc.); or it is used in combination as wall material, also have and use coating embedding or multilayer embedding protection activity of probiotic.
But although wall material used at present and embedding techniques and method obtain higher embedding rate and protection preferably; but have that single wall material protective capability is poor, mixing wall material cost is higher; complicated operation, the shortcomings such as fixed point releasability is strong, the rear probio subalimentation of release.So invent that a kind of cost is low, embedding rate better, can probiotic active be protected and the wall material of tool fixed point releasability is the problem needing to solve.
Summary of the invention
For above problem; the present invention proposes albumen and reproducibility compound sugar Mei Lade product to embed probio as microcapsule wall material, protects it active and improve microcapsules fixed point releasability and surely grow desired nutritional after providing probio release under the condition such as hydrochloric acid in gastric juice, cholate.
Because lactalbumin tool comfort cushioning ability better can protect probio, non-newborn basal wall material microcapsules are applied limited, so the present invention selects lactalbumin from albumen in the food such as infant formula in addition.Oligoisomaltose is a kind of in functional oligose, can breed the effect that Bifidobacterium etc. plays beneficial bacterium.The more important thing is that oligoisomaltose molecular end has reduction group, heat can be total to protein or amino acid and Maillard reaction occurs.Because oligoisomaltose reducing end is at sugar-chain end, be grafted on protein chain, the functional characters such as the gelation of albumen, film forming, emulsibility, anti-digestibility can be increased, this graft product is particularly suitable as microcapsule wall material embedding probio, because emulsibility is higher, can obtain rounder and more smooth mode of appearance by emulsion process, embedding efficiency is higher.Wall material antipepsin digestion power can protect probio to arrive enteron aisle better, and after enteron aisle release, wall material can also grow required nutrition by supply probio surely, avoids excreting owing to can not surely grow causing probio to waste.
Lactalbumin used in the present invention is WPC WPC-80 or whey isolate protein; Oligoisomaltose is IMO900.
Lactalbumin and reproducibility compound sugar Mei Lade product microcapsule wall material is characterized in that: it is 8-12% solution that lactalbumin and oligoisomaltose are mixed with protein content according to mass ratio 1:1-10:1, and adjustment pH is 7.6-8.0,4 DEG C of refrigerator overnight.85-90 DEG C of water bath with thermostatic control heating 2-4 hour, cooling.Be wall material solution.
Preferably, described Maillard reaction graft product probiotic microcapsule wall material, be that to be mixed with protein content by lactalbumin and oligoisomaltose according to mass ratio 2:1-5:1 be 10-12% solution, adjustment pH is 7.6-7.8,4 DEG C of refrigerator overnight.85 DEG C of water bath with thermostatic control heating 2-3 hour, cooling.
Albumen and reproducibility compound sugar Mei Lade product microcapsule wall material, wall material raw material is albumen and prebiotics, tool nutritive peculiarity and functional characteristic.Albumen and oligoisomaltose Mei Lade product microcapsule wall material, there is security high, have no side effect, emulsibility be good, good film-forming property and the advantage such as economical and practical, alternative conventional wall material is applied to embedding bioactivator, as biologically active peptide, oxidizable grease etc., replaceable or part replaces existing wall material.This wall material is particularly suitable for embedding probio, and because it has non-oxidizability and compact structure can strengthen protective capability, releasability of fixing a point under enteron aisle enzyme and enteric microorganism existence condition strengthens, and release rear wall material material can be probio provides nutrition.
The present invention also provides two kinds of methods using above-mentioned U.S. rad graft product wall material embedding probio, as follows:
1. emulsion process makes probiotic microcapsule
(1) core collecting cells;
(2) wall material solution mixes with core, adds coagulating agent;
(3) mixed solution adding coagulating agent is poured into mixed emulsion, temperature constant magnetic stirring;
(4) high speed freezing centrifuge, centrifuge washing.
In described step (1), probio activates, and centrifugal concentrating makes probio total content 10
9-11cFU/g, probio is have one or two or more kinds in the Lactobacillus plantarum of prebiotic effect, Lactobacillus rhamnosus, Lactobacillus casei.
In described step (2), get wall material solution and mix with the suspension bacteria liquid 10:1-50:1 in (1), add 3.5g/L glucolactone (GDL) and 0.1M CaCl
2coagulating agent, stirs evenly fast.
In described step (3), mixed liquor adds in vegetable oil and Span-80 or Tween-80 a kind of according to 5:1-10:1 mixing, and mixed liquor and mixed emulsion ratio are 1:10-1:50,40 DEG C of magnetic agitation 2h.
In described step (4), pour in centrifuge tube by the solution after stirring 2h in (3), at 4 DEG C, under 1000g condition, centrifugal 5 min, discard upper oil phase, add appropriate physiological saline concussion, again centrifugal, repeat 3 times.
As wanted long-term storage, need step (4) to obtain wet microcapsules freeze-drying 4 DEG C placement.
2. spray drying process makes probiotic microcapsule
(1) core collecting cells;
(2) wall material solution mixes with core;
(3) spraying dry.
In described step (3); by spray-dried for (2) wall material core mixed liquor; spraying dry various process parameters is determined: EAT is 130-150 DEG C by single factor experiment; feed rate is 10.00-20.00mL/min; because raw material has lactalbumin and oligoisomaltose; can serve as hat resistant pretectants, the vigor reducing probio declines.
Described lactalbumin and oligoisomaltose Mei Lade product wall material and properties of microcapsules detect and comprise following several:
A. Maillard reaction level determinations
OPA method is utilized to measure free amino group content, accurately taking 40mg OPA (OPA) is dissolved in the methyl alcohol of 1 mL, add SDS solution 2.5 mL, 0.1M borax soln 25mL, the 100 μ L beta-mercaptoethanols of 20% (w/v) respectively, finally be settled to 50mL with distilled water, this is OPA reagent.During mensuration, get OPA reagent 4 mL in test tube, inject 200 μ L sample liquid respectively, under 35oC reacts 2min, 340nm, survey its light absorption value after mixing, to add 200 μ L water for blank sample in OPA reagent, the difference Δ A of the two is the clean light absorption value of free amino group.With undressed lactalbumin for standard, calculate the relative percentage of each sample free ammonia base content.
B. microcapsule embedded rate measures
Calculate probiotic microcapsule embedding rate according to following formulae discovery:
Wherein, C1 is bacteria suspension bacteria containing amount ratio; C2 is that microcapsule suspensions contains bacterium ratio.
Bacterium vitality test in microcapsules: get 0.5g microcapsules, adds the citric acid solution of 4.5mL 50mM, at homogenizer 10000r/min 45s, breaks microcapsules release bacterium.Continuous 10 times of dilutions, get solution 100 μ L spread plate method and cultivate 48h, calculate viable count.
C. microcapsules acid resistance measures
Simulated gastric fluid (SGJ): regulate NaCl solution (0.2% w/v) pH value to be 2.0 with dense HCl, add pepsin and make its concentration be 0.3 g/L, finally carry out filtration sterilization with the film of 0.22 μm, this solution is now with the current.
Get in the test tube that 0.5g microcapsules join containing the SGF (SGF) of 4.5mL, under 37oC constant temperature, cultivate 90 min.Adopt continuous 10 times of dilute solutions, get solution 100 μ L spread plate method and cultivate 48h, calculate viable count.
D. the release characteristics of microcapsules
Get in the test tube that 0.5g microcapsules join containing the simulated intestinal fluid (SIF) of 4.5mL, at 37oC constant-temperature table 100r/min.Taken out of analysis every 15min sample.Continuous 10 times of dilutions, get solution 100 μ L plate count, and spread plate method cultivates 48h, calculate viable count, when continuous two sample point bacterium numbers change difference is little, writes down previous sampling time point and are the time that microcapsule life tonifying bacterium all discharges.
the advantage that the present invention has:
(1) selected raw material itself has been newborn base albumen and prebiotics, tool nutritive peculiarity and functional characteristic, and its Maillard reaction adopts wet method reaction, and reaction condition is simple, easy to operate.
(2) Maillard reaction graft product is that wall material makes the fine and close protective capability enhancing of microcapsule structure.Releasability of fixing a point under enteron aisle enzyme and enteric microorganism existence condition strengthens, and release rear wall material material can be probio provides nutrition.
(3) probiotic microcapsule that prepared by the present invention adopts the emulsion process preparation of Inner source, adopts epoxy resin to avoid making the death of probio in microcapsules process to a great extent, produces microcapsules and can be directly used in food processing or freezing, freeze-drying preservation, easy to use.
(4) spray drying process is simple to operate, produces Microcapsules Size uniformity.
Detailed description of the invention
In following examples, material composition used unless otherwise noted, is commercially available prod.Raw material WPC WPC-80 and oligoisomaltose IMO900 stores at 4 DEG C.Lactobacillus rhamnosus LGG is business probio, purchased from Beijing Hansen Corp. of section.
Example 1
WPC is mixed with 10%(w/v) solution, add the oligoisomaltose of albumen quality 1/4, adjust pH to 7.6 with the HCl of NaOH and 1M of 10M, 4 DEG C of refrigerator overnight, make its abundant hydration.85 DEG C keep 3h, and make lactalbumin and the grafting of oligoisomaltose generating portion, reactant liquor is wall material solution.Be cooled to room temperature, 4 DEG C store for future use.
Preparation core material solution, by Lactobacillus rhamnosus LGG separation and purification, and activated for two generations, get nutrient solution at 4 DEG C, the centrifugal 5min of 5000r/min, gets bacterium mud, is dissolved in physiological saline, makes bacteria concentration reach 10
9about CFU/g.
Prepare mixed emulsion, get soybean oil and span-80 by volume 10:1 mix.Be heated to 40 DEG C.Magnetic agitation speed is 700r/min.
Wall material solution and bacteria suspension are pressed the mixing of 10:1 volume ratio, add 3.5g/L glucolactone (GDL) and 0.1M CaCl2 coagulating agent.Stir evenly rapidly.Join in the mixing and emulsifying solution of magnetic agitation 40 DEG C.Constant temperature stirs 2h.
Poured into by emulsified solution in centrifuge tube, at 4 DEG C, under 1000g condition, centrifugal 5min, discards upper oil phase, adds appropriate physiological saline concussion, again centrifugal, repeats 3 times.Remove supernatant, namely obtain probiotic microcapsule.
Detection obtains following index:
Lactalbumin and oligoisomaltose Mei Lade product wall material free amino group content are 89.65%; Embedding rate can reach 89.53%; In the SGF of pH=2.0,90min vigor is 7.52Log
10cFU/g; In simulated intestinal fluid, complete release time is 60min.
Example 2
WPC is mixed with 10%(w/v) solution, add the oligoisomaltose of albumen quality 1/2, adjust pH to 7.6 with the HCl of NaOH and 1M of 10M, 4 DEG C of refrigerator overnight, make its abundant hydration.85 DEG C keep 3h, and make lactalbumin and the grafting of oligoisomaltose generating portion, reactant liquor is wall material solution.Be cooled to room temperature, 4 DEG C store for future use.
Preparation core material solution, by Lactobacillus rhamnosus LGG separation and purification, and activated for two generations, get nutrient solution at 4 DEG C, the centrifugal 5min of 5000r/min, gets bacterium mud, is dissolved in physiological saline, makes bacteria concentration reach 10
9about CFU/g.
Wall material solution and bacteria suspension are pressed the mixing of 10:1 volume ratio, adding 4.0g/L glucolactone (GDL) is coagulating agent.Stir evenly rapidly.Join in the soybean oil of magnetic agitation 40 DEG C, constant temperature stirs 2h.
Poured into by emulsified solution in centrifuge tube, at 4 DEG C, under 1000g condition, centrifugal 5min, discards upper oil phase, adds appropriate physiological saline concussion, again centrifugal, repeats 3 times.Remove supernatant, namely obtain probiotic microcapsule.
Detection obtains following index:
Lactalbumin and oligoisomaltose Mei Lade product wall material free amino group content are 86.32%; Embedding rate can reach 87.2%; In the SGF of pH=2.0,90min vigor is 7.2 Log
10cFU/g; In simulated intestinal fluid, complete release time is 75min.
In above embodiment: WPC and oligoisomaltose mass ratio can be chosen arbitrarily from 1:1-10:1; Coagulating agent can by glucolactone (GDL) and CaCl
2in one or mixing form; Oil phase is made up of soybean oil and Span-80 or Tween-80, also can not add Span-80 and Tween-80.
Example 3
WPC is mixed with 10%(w/v) solution, add the oligoisomaltose of albumen quality 1/4, adjust pH to 7.6 with the HCl of NaOH and 1M of 10M, 4 DEG C of refrigerator overnight, make its abundant hydration.85 DEG C keep 3h, and make lactalbumin and the grafting of oligoisomaltose generating portion, reactant liquor is wall material solution.Be cooled to room temperature, 4 DEG C store for future use.
Preparation core material solution, by Lactobacillus rhamnosus LGG separation and purification, and activated for two generations, get nutrient solution at 4 DEG C, the centrifugal 5min of 5000r/min, gets bacterium mud, is dissolved in physiological saline, makes bacteria concentration reach 10
10about CFU/g.
Adopt spray drying process to make microcapsules, EAT is 150 DEG C, and feed rate is 20.00mL/min, obtained dry microcapsules.Detection obtains following index:
Lactalbumin and oligoisomaltose Mei Lade product wall material free amino group content are 89.32%; Embedding rate can reach 70.23%, and after rehydration, in the SGF of ph=2.0,90min vigor is 7.02Log
10cFU/g; After rehydration, in simulated intestinal fluid, complete release time is 75min.
Claims (7)
1. lactalbumin and a reproducibility compound sugar Mei Lade product microcapsule wall material, is characterized in that: it is 8-12% solution that lactalbumin and oligoisomaltose are mixed with protein content according to mass ratio 1:1-10:1, and adjustment pH is 7.6-8.0,4 DEG C of refrigerator overnight; 85-90 DEG C of water bath with thermostatic control heating 2-4h, make generation differential responses degree Maillard reaction, generation contains the graft of different content as wall material solution.
2. U.S. rad product microcapsule wall material according to claim 1, is characterized in that: it is 10-12% solution that lactalbumin and oligoisomaltose are mixed with protein content according to mass ratio 2:1-5:1, and adjustment pH is 7.6-7.8,4 DEG C of refrigerator overnight; 85 DEG C of water bath with thermostatic control heating 2-3 hour, cooling.
3. a Mei Lade product microcapsule wall material as claimed in claim 1 or 2 is applied in probiotic microcapsule, it is characterized in that: by above-mentioned U.S. rad product microcapsule wall material solution with probiotics bacterial suspension by volume 10:1-50:1 mix, in bacteria suspension, probio total content is 10
10-11cFU/g, probio is have one or two or more kinds in the Lactobacillus plantarum of prebiotic effect, Lactobacillus rhamnosus, Lactobacillus casei; The content of often kind of bacterium is 10
8-11cFU/g.
4. U.S. rad product microcapsule wall material according to claim 3 is applied in probiotic microcapsule, it is characterized in that: by above-mentioned U.S. rad product microcapsule wall material solution, mix with bacteria suspension volume ratio 10:1-50:1 according to wall material solution, to pour into after finally above-mentioned mixed solution being added coagulating agent in oil phase to wash after constant temperature stirs 2h and centrifugally make probiotic microcapsule.
5. apply in probiotic microcapsule according to claim 4 U.S. rad product microcapsule wall material, it is characterized in that: coagulating agent is for adding 2.0-4.5g/L glucolactone (GDL), 0.1-0.2 M CaCl
2in one or two kinds; Oil phase is made up of soybean oil or is mixed according to 5:1-10:1 by a kind of in soybean oil and Span-80 or Tween-80.
6. apply in probiotic microcapsule according to claim 4 U.S. rad product microcapsule wall material, it is characterized in that: the wall material adding coagulating agent mixes according to 1:50-1:10 with oil phase with bacteria suspension mixed liquor, 40 DEG C of temperature constant magnetic stirring 300-900 r/min, at 4 DEG C of refrigerated centrifuges after 2h, the centrifugal 5min of 1000g, with centrifugal twice of physiological saline cleaning; Namely wet probiotic microcapsule is obtained.
7. U.S. rad product microcapsule wall material according to claim 6 is applied in probiotic microcapsule, it is characterized in that: the probio micro-capsule product obtained can be put into milk, yogurt, cheese, jelly, ice cream or milk powder food and make functional food containing probio, is applied to livestock and poultry cultivation as feed addictive; Or adopt auxiliary material to continue to be processed into tablet or filling one-tenth large capsule by the interpolation acceptable auxiliary material of other drug or field of food.
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