CN104543366B - The preparation method of feeding selenium rich of egg white matter - Google Patents
The preparation method of feeding selenium rich of egg white matter Download PDFInfo
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- CN104543366B CN104543366B CN201410857899.9A CN201410857899A CN104543366B CN 104543366 B CN104543366 B CN 104543366B CN 201410857899 A CN201410857899 A CN 201410857899A CN 104543366 B CN104543366 B CN 104543366B
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- 239000011669 selenium Substances 0.000 title claims abstract description 113
- BUGBHKTXTAQXES-UHFFFAOYSA-N Selenium Chemical compound [Se] BUGBHKTXTAQXES-UHFFFAOYSA-N 0.000 title claims abstract description 102
- 229910052711 selenium Inorganic materials 0.000 title claims abstract description 102
- QCVGEOXPDFCNHA-UHFFFAOYSA-N 5,5-dimethyl-2,4-dioxo-1,3-oxazolidine-3-carboxamide Chemical compound CC1(C)OC(=O)N(C(N)=O)C1=O QCVGEOXPDFCNHA-UHFFFAOYSA-N 0.000 title claims abstract description 41
- 102000002322 Egg Proteins Human genes 0.000 title claims abstract description 41
- 108010000912 Egg Proteins Proteins 0.000 title claims abstract description 41
- 235000014103 egg white Nutrition 0.000 title claims abstract description 41
- 210000000969 egg white Anatomy 0.000 title claims abstract description 41
- 238000002360 preparation method Methods 0.000 title claims abstract description 15
- 239000007921 spray Substances 0.000 claims abstract description 43
- 235000017587 Medicago sativa ssp. sativa Nutrition 0.000 claims abstract description 32
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 23
- 241000219823 Medicago Species 0.000 claims abstract description 21
- 244000250129 Trigonella foenum graecum Species 0.000 claims abstract description 21
- 235000001484 Trigonella foenum graecum Nutrition 0.000 claims abstract description 21
- 235000001019 trigonella foenum-graecum Nutrition 0.000 claims abstract description 21
- 239000000706 filtrate Substances 0.000 claims abstract description 18
- 238000001816 cooling Methods 0.000 claims abstract description 9
- 238000001035 drying Methods 0.000 claims abstract description 9
- 239000013049 sediment Substances 0.000 claims abstract description 9
- 238000005507 spraying Methods 0.000 claims description 12
- 240000004658 Medicago sativa Species 0.000 claims description 11
- 239000002994 raw material Substances 0.000 claims description 10
- 239000002002 slurry Substances 0.000 claims description 10
- PUKLDDOGISCFCP-JSQCKWNTSA-N 21-Deoxycortisone Chemical compound C1CC2=CC(=O)CC[C@]2(C)[C@@H]2[C@@H]1[C@@H]1CC[C@@](C(=O)C)(O)[C@@]1(C)CC2=O PUKLDDOGISCFCP-JSQCKWNTSA-N 0.000 claims description 8
- 241000196324 Embryophyta Species 0.000 claims description 8
- FCYKAQOGGFGCMD-UHFFFAOYSA-N Fulvic acid Natural products O1C2=CC(O)=C(O)C(C(O)=O)=C2C(=O)C2=C1CC(C)(O)OC2 FCYKAQOGGFGCMD-UHFFFAOYSA-N 0.000 claims description 8
- 239000012378 ammonium molybdate tetrahydrate Substances 0.000 claims description 8
- FIXLYHHVMHXSCP-UHFFFAOYSA-H azane;dihydroxy(dioxo)molybdenum;trioxomolybdenum;tetrahydrate Chemical compound N.N.N.N.N.N.O.O.O.O.O=[Mo](=O)=O.O=[Mo](=O)=O.O=[Mo](=O)=O.O=[Mo](=O)=O.O[Mo](O)(=O)=O.O[Mo](O)(=O)=O.O[Mo](O)(=O)=O FIXLYHHVMHXSCP-UHFFFAOYSA-H 0.000 claims description 8
- YYRMJZQKEFZXMX-UHFFFAOYSA-L calcium bis(dihydrogenphosphate) Chemical compound [Ca+2].OP(O)([O-])=O.OP(O)([O-])=O YYRMJZQKEFZXMX-UHFFFAOYSA-L 0.000 claims description 8
- 229940062672 calcium dihydrogen phosphate Drugs 0.000 claims description 8
- 229910000389 calcium phosphate Inorganic materials 0.000 claims description 8
- 238000005520 cutting process Methods 0.000 claims description 8
- 239000004744 fabric Substances 0.000 claims description 8
- 239000003337 fertilizer Substances 0.000 claims description 8
- 239000002509 fulvic acid Substances 0.000 claims description 8
- 229940095100 fulvic acid Drugs 0.000 claims description 8
- SURQXAFEQWPFPV-UHFFFAOYSA-L iron(2+) sulfate heptahydrate Chemical compound O.O.O.O.O.O.O.[Fe+2].[O-]S([O-])(=O)=O SURQXAFEQWPFPV-UHFFFAOYSA-L 0.000 claims description 8
- 229940099596 manganese sulfate Drugs 0.000 claims description 8
- 239000011702 manganese sulphate Substances 0.000 claims description 8
- 235000007079 manganese sulphate Nutrition 0.000 claims description 8
- SQQMAOCOWKFBNP-UHFFFAOYSA-L manganese(II) sulfate Chemical compound [Mn+2].[O-]S([O-])(=O)=O SQQMAOCOWKFBNP-UHFFFAOYSA-L 0.000 claims description 8
- 235000019691 monocalcium phosphate Nutrition 0.000 claims description 8
- NWONKYPBYAMBJT-UHFFFAOYSA-L zinc sulfate Chemical compound [Zn+2].[O-]S([O-])(=O)=O NWONKYPBYAMBJT-UHFFFAOYSA-L 0.000 claims description 8
- 229910000368 zinc sulfate Inorganic materials 0.000 claims description 8
- 239000011686 zinc sulphate Substances 0.000 claims description 8
- 235000009529 zinc sulphate Nutrition 0.000 claims description 8
- DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical compound C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 claims description 4
- 239000011734 sodium Substances 0.000 claims description 4
- 229910052708 sodium Inorganic materials 0.000 claims description 4
- 238000007796 conventional method Methods 0.000 claims description 2
- 238000005360 mashing Methods 0.000 claims description 2
- MCAHWIHFGHIESP-UHFFFAOYSA-N selenous acid Chemical compound O[Se](O)=O MCAHWIHFGHIESP-UHFFFAOYSA-N 0.000 claims 1
- 229940091258 selenium supplement Drugs 0.000 abstract description 98
- 102000004169 proteins and genes Human genes 0.000 abstract description 22
- 108090000623 proteins and genes Proteins 0.000 abstract description 22
- 244000144972 livestock Species 0.000 abstract description 11
- 238000000751 protein extraction Methods 0.000 abstract description 8
- 244000144977 poultry Species 0.000 abstract description 6
- 239000000463 material Substances 0.000 abstract description 5
- 102000008114 Selenoproteins Human genes 0.000 abstract description 4
- 108010074686 Selenoproteins Proteins 0.000 abstract description 4
- 230000036039 immunity Effects 0.000 abstract description 4
- 238000004519 manufacturing process Methods 0.000 abstract description 4
- 235000016709 nutrition Nutrition 0.000 abstract description 4
- 108010066207 Poultry Proteins Proteins 0.000 abstract description 3
- 230000000694 effects Effects 0.000 abstract description 3
- 230000035764 nutrition Effects 0.000 abstract description 3
- 229940088597 hormone Drugs 0.000 abstract description 2
- 239000005556 hormone Substances 0.000 abstract description 2
- 231100000957 no side effect Toxicity 0.000 abstract description 2
- 231100000252 nontoxic Toxicity 0.000 abstract description 2
- 230000003000 nontoxic effect Effects 0.000 abstract description 2
- 235000018102 proteins Nutrition 0.000 description 20
- 239000011781 sodium selenite Substances 0.000 description 9
- 241001465754 Metazoa Species 0.000 description 8
- BVTBRVFYZUCAKH-UHFFFAOYSA-L disodium selenite Chemical compound [Na+].[Na+].[O-][Se]([O-])=O BVTBRVFYZUCAKH-UHFFFAOYSA-L 0.000 description 8
- 235000013336 milk Nutrition 0.000 description 8
- 239000008267 milk Substances 0.000 description 8
- 210000004080 milk Anatomy 0.000 description 8
- 229960001471 sodium selenite Drugs 0.000 description 8
- 235000015921 sodium selenite Nutrition 0.000 description 8
- 244000025254 Cannabis sativa Species 0.000 description 7
- RYGMFSIKBFXOCR-UHFFFAOYSA-N Copper Chemical compound [Cu] RYGMFSIKBFXOCR-UHFFFAOYSA-N 0.000 description 6
- NINIDFKCEFEMDL-UHFFFAOYSA-N Sulfur Chemical compound [S] NINIDFKCEFEMDL-UHFFFAOYSA-N 0.000 description 6
- 239000005864 Sulphur Substances 0.000 description 6
- 239000010949 copper Substances 0.000 description 6
- 229910052802 copper Inorganic materials 0.000 description 6
- 239000007788 liquid Substances 0.000 description 6
- 241000287828 Gallus gallus Species 0.000 description 5
- 238000000034 method Methods 0.000 description 4
- 150000008107 benzenesulfonic acids Chemical class 0.000 description 3
- 238000002474 experimental method Methods 0.000 description 3
- 239000011655 sodium selenate Substances 0.000 description 3
- 206010039921 Selenium deficiency Diseases 0.000 description 2
- RJFAYQIBOAGBLC-BYPYZUCNSA-N Selenium-L-methionine Chemical compound C[Se]CC[C@H](N)C(O)=O RJFAYQIBOAGBLC-BYPYZUCNSA-N 0.000 description 2
- RJFAYQIBOAGBLC-UHFFFAOYSA-N Selenomethionine Natural products C[Se]CCC(N)C(O)=O RJFAYQIBOAGBLC-UHFFFAOYSA-N 0.000 description 2
- 210000004027 cell Anatomy 0.000 description 2
- 238000011161 development Methods 0.000 description 2
- 235000013601 eggs Nutrition 0.000 description 2
- 150000003839 salts Chemical class 0.000 description 2
- 229960002718 selenomethionine Drugs 0.000 description 2
- 239000002689 soil Substances 0.000 description 2
- FDKWRPBBCBCIGA-REOHCLBHSA-N (2r)-2-azaniumyl-3-$l^{1}-selanylpropanoate Chemical compound [Se]C[C@H](N)C(O)=O FDKWRPBBCBCIGA-REOHCLBHSA-N 0.000 description 1
- PMYDPQQPEAYXKD-UHFFFAOYSA-N 3-hydroxy-n-naphthalen-2-ylnaphthalene-2-carboxamide Chemical compound C1=CC=CC2=CC(NC(=O)C3=CC4=CC=CC=C4C=C3O)=CC=C21 PMYDPQQPEAYXKD-UHFFFAOYSA-N 0.000 description 1
- 241000251468 Actinopterygii Species 0.000 description 1
- 206010003694 Atrophy Diseases 0.000 description 1
- FDKWRPBBCBCIGA-UWTATZPHSA-N D-Selenocysteine Natural products [Se]C[C@@H](N)C(O)=O FDKWRPBBCBCIGA-UWTATZPHSA-N 0.000 description 1
- 241000272496 Galliformes Species 0.000 description 1
- 244000068988 Glycine max Species 0.000 description 1
- 235000010469 Glycine max Nutrition 0.000 description 1
- 206010053759 Growth retardation Diseases 0.000 description 1
- GUBGYTABKSRVRQ-QKKXKWKRSA-N Lactose Natural products OC[C@H]1O[C@@H](O[C@H]2[C@H](O)[C@@H](O)C(O)O[C@@H]2CO)[C@H](O)[C@@H](O)[C@H]1O GUBGYTABKSRVRQ-QKKXKWKRSA-N 0.000 description 1
- 229910003424 Na2SeO3 Inorganic materials 0.000 description 1
- 240000002853 Nelumbo nucifera Species 0.000 description 1
- 235000006508 Nelumbo nucifera Nutrition 0.000 description 1
- 235000006510 Nelumbo pentapetala Nutrition 0.000 description 1
- 206010030113 Oedema Diseases 0.000 description 1
- 244000046052 Phaseolus vulgaris Species 0.000 description 1
- 235000010627 Phaseolus vulgaris Nutrition 0.000 description 1
- 240000004808 Saccharomyces cerevisiae Species 0.000 description 1
- 230000002159 abnormal effect Effects 0.000 description 1
- 230000000743 anti-peroxide Effects 0.000 description 1
- 230000037444 atrophy Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
- 230000037396 body weight Effects 0.000 description 1
- 230000015556 catabolic process Effects 0.000 description 1
- 238000005119 centrifugation Methods 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- ARUVKPQLZAKDPS-UHFFFAOYSA-L copper(II) sulfate Chemical compound [Cu+2].[O-][S+2]([O-])([O-])[O-] ARUVKPQLZAKDPS-UHFFFAOYSA-L 0.000 description 1
- 229910000366 copper(II) sulfate Inorganic materials 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 230000007850 degeneration Effects 0.000 description 1
- 235000005911 diet Nutrition 0.000 description 1
- 230000037213 diet Effects 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 208000035475 disorder Diseases 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 235000013861 fat-free Nutrition 0.000 description 1
- 238000001914 filtration Methods 0.000 description 1
- 239000004459 forage Substances 0.000 description 1
- 231100000001 growth retardation Toxicity 0.000 description 1
- 238000001727 in vivo Methods 0.000 description 1
- 239000008101 lactose Substances 0.000 description 1
- 201000010260 leiomyoma Diseases 0.000 description 1
- 210000005229 liver cell Anatomy 0.000 description 1
- 230000004060 metabolic process Effects 0.000 description 1
- 239000011785 micronutrient Substances 0.000 description 1
- 235000013369 micronutrients Nutrition 0.000 description 1
- 210000003205 muscle Anatomy 0.000 description 1
- 230000003387 muscular Effects 0.000 description 1
- 230000017074 necrotic cell death Effects 0.000 description 1
- 239000002245 particle Substances 0.000 description 1
- 239000008188 pellet Substances 0.000 description 1
- 150000002978 peroxides Chemical class 0.000 description 1
- 230000035479 physiological effects, processes and functions Effects 0.000 description 1
- 229920001184 polypeptide Polymers 0.000 description 1
- 239000000843 powder Substances 0.000 description 1
- 238000001556 precipitation Methods 0.000 description 1
- 125000002924 primary amino group Chemical group [H]N([H])* 0.000 description 1
- 102000004196 processed proteins & peptides Human genes 0.000 description 1
- 108090000765 processed proteins & peptides Proteins 0.000 description 1
- 239000000047 product Substances 0.000 description 1
- 230000001850 reproductive effect Effects 0.000 description 1
- ZKZBPNGNEQAJSX-UHFFFAOYSA-N selenocysteine Natural products [SeH]CC(N)C(O)=O ZKZBPNGNEQAJSX-UHFFFAOYSA-N 0.000 description 1
- 229940055619 selenocysteine Drugs 0.000 description 1
- 235000016491 selenocysteine Nutrition 0.000 description 1
- 235000018716 sodium selenate Nutrition 0.000 description 1
- 229960001881 sodium selenate Drugs 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 210000000952 spleen Anatomy 0.000 description 1
- 239000013589 supplement Substances 0.000 description 1
- 230000001988 toxicity Effects 0.000 description 1
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- 210000004885 white matter Anatomy 0.000 description 1
- 208000024877 white muscle disease Diseases 0.000 description 1
Landscapes
- Fodder In General (AREA)
Abstract
The present invention relates to a kind of preparation method of feeding selenium rich of egg white matter.Its step is first to spray alfalfa with micro- rich water solution selenium-rich light violet russian fenugreek herb is made, then by the light violet russian fenugreek herb of selenium-rich through being beaten, squeezing and obtaining filtrate, by filtrate at 55~85 DEG C heated at constant temperature, centrifuged after cooling, gained sediment is drying to obtain selenium rich of egg white matter.Feeding selenium rich of egg white matter produced by the present invention is natural selenoprotein, and its Se content is up to 4.5~30mg/kg；Used cost of material is low and Protein Extraction yield and purity are higher, and the protein yields of the light violet russian fenugreek herb of selenium-rich are 51~63%, and lipidated protein is up to 68~76%；Feeding selenium rich of egg white matter Selenium Supplement while livestock and poultry proteins necessary is supplemented, have nutrition and health care, strengthen immunity, improve Production of Livestock and Poultry power the effect of, and without any hormone, it is nontoxic, have no side effect, it is safe to use.
Description
Technical field：
It is more particularly to a kind of using selenium-enriched alfalfa as the feeding of raw material the present invention relates to a kind of preparation method of protein
The preparation method of selenium rich of egg white matter.
Background technology：
Selenium (Se) is the required micronutrient element of animal, is played an important role in animal body metabolism,
Selenium deficiency can cause the reduction of animal body inner cell antiperoxide toxicity ability, and cell oedema is occurred, gone out by peroxide breakdown
Blood, exudative diathesis, necrosis of liver cells, spleen fibroid atrophy, muscle is abnormal, muscular degeneration of heart；Show as white muscle disease, physiology
Disorder, growth retardation etc..Due to weather and soil, there is 2/3 soil selenium deficiency in China, causes selenium in animal forage grass
The shortage of element, therefore, it is necessary to add selenium into animal feed, inorganic salts are directly added presently mainly into animal feed,
Such as sodium selenite (Na2SeO3), sodium selenate (Na2SeO4Or Na2SeO4·10H2O) or organic preparation, such as selenomethionine and
Strengthen selenium yeast etc..Although the inorganic high cost of Se content is low, requirement and dosis toxica difference very little, easily it is poisoned, is producing
Upper application security is poor；Although organic preparation is higher using safety, good absorbing, complex manufacturing, price than inorganic salts.
Protein is the material base of life, does not just have life without protein.If protein content in livestock and poultry diet
Deficiency, growth will be limited, and Body weight loss, efficiency of feed utilization reduces, and reproductive capability is disorderly.Therefore, to meet livestock and poultry normal growth
The nutritional need of development, generally require to add a certain amount of protein feeds in feed for livestock, such as beans, puffed soybean, fish
Powder etc., but the lipidated protein of these protein feeds is low, and addition precision prescribed is high.
Therefore, it is significant to develop a kind of safe and efficient high-purity feed protein matter rich in Organic Selenium.
Alfalfa (Medicaco sativa L.) is a kind of high-quality feed resource, not only contains higher protein,
And there is very strong selenium rich ability, and Enriching Selenium mainly exists in the form of selenoprotein in vivo.Using alfalfa to inorganic
The high conversion and high protein characteristic of selenium, are acted on by mass transfer, inorganic selenium are converted into active Selenonic protein
Matter, then separated out the selenium rich of egg white matter in selenium-enriched alfalfa with thermal precipitation method.
At present, the pertinent literature report that selenium rich of egg white matter is extracted from selenium-enriched alfalfa is had not yet seen.
The content of the invention：
The present invention provides a kind of method that selenium rich of egg white matter is prepared using the light violet russian fenugreek herb of selenium-rich as raw material.Prepared selenium-rich
Selenium Supplement while protein is used to supplement livestock and poultry proteins necessary, there is nutrition and health care, strengthen immunity, improve Production of Livestock and Poultry
The function of power.
The present invention is to be achieved through the following technical solutions foregoing invention purpose：
A kind of feeding selenium rich of egg white matter preparation method is designed, is comprised the following steps：
(1) selenium-enriched alfalfa produces：Alfalfa is cultivated according to a conventional method, in Growth of Alfalfa to 4~6cm of plant height
When, start to micro- rich water solution that its spraying concentration is 110~240mg/kg, the amount of spraying is 45kg/667m2, when afternoon 5 with
After spray, sprayed once every 4 days, even spray 4 times, received within the 5th day after the 4th sprays such as rain, it is necessary to respray after spraying
Cut, produce the light violet russian fenugreek herb of selenium-rich；
Above-mentioned micro- fertilizer is made up of the raw material of following parts by weight：10~15 parts of ammonium molybdate tetrahydrate, white vitriol 17~22
Part, 12~17 parts of cupric sulfate pentahydrate, 16~21 parts of ferrous sulfate heptahydrate, 9~15 parts of manganese sulfate, 15~20 parts of calcium dihydrogen phosphate,
10~100 parts of sodium selenite, 16~20 parts of fulvic acid, 5~10 parts of neopelex.
(2) selenium rich of egg white matter is extracted：The above-mentioned light violet russian fenugreek herb of gained selenium-rich is rinsed well, shredded with cutting machine, then to
It adds the water of 3~7 times of volumes, is placed in 20~60min of mashing in beater, then squeezes out slurries with squeezer, then with double
Layer filter-cloth filtering slurries, collect filtrate, then by filtrate at 55~85 DEG C 20~60min of heated at constant temperature, centrifugation is placed in after cooling
Centrifuged in machine with 4000~6000 revs/min of rotating speed, gained sediment is drying to obtain selenium rich of egg white matter.
The present invention has following positive beneficial technique effect：
1. the selenium rich of egg white matter that the inventive method is prepared is the natural selenoprotein in selenium-enriched alfalfa, wherein selenium
Combined with selenocysteine and its derivative, selenomethionine and its derivative or certain intermolecular force with protein
Organic Selenium (material containing selenium is mainly selenoprotein, polypeptide containing selenium, amino containing selenium etc.).Prepared selenium rich of egg white matter selenium contains
Amount is up to 4.5~30mg/kg.
2. use cost of material low and Protein Extraction yield and purity are higher, the protein production of the light violet russian fenugreek herb of selenium-rich
Rate is 51~63%, and lipidated protein is up to 68~76%.
3. the selenium rich of egg white matter prepared with selenium-enriched alfalfa Selenium Supplement while livestock and poultry proteins necessary is supplemented, has
Nutrition and health care, strengthen immunity, improve Production of Livestock and Poultry power the effect of, and without any hormone, it is nontoxic, have no side effect, it is safe to use.
4. also the selenium rich of egg white matter can be added in animal and fowl fodder, selenium-rich livestock and fowls livestock products are produced, such as selenium rich of egg, selenium-rich
Poultry etc..
Embodiment：
Embodiment 1：A kind of preparation method of feeding selenium rich of egg white matter, comprises the following steps：
(1) selenium-enriched alfalfa produces：In 2012 in the alfalfa life of Yellow River in Henan Province Tan Hebo grass cultivation Co., Ltd
Producing region, when Growth of Alfalfa is to 4~6cm of plant height, start, to micro- rich water solution that its spraying concentration is 110mg/kg, to spray
Measure as 45kg/667m2, when afternoon 5 after spray, sprayed once every 4 days, even spray 4 times, spray after such as rain, it is necessary to weigh
Spray, is gathered in, produces the light violet russian fenugreek herb of selenium-rich for the 5th day after the 4th sprays (Se content is 1.6mg/kg after measured).
Micro- fertilizer is made up of the raw material of following parts by weight：10 parts of ammonium molybdate tetrahydrate, 17 parts of white vitriol, five water sulphur
Sour 12 parts of copper, 16 parts of ferrous sulfate heptahydrate, 9 parts of manganese sulfate, 15 parts of calcium dihydrogen phosphate, 10 parts of sodium selenite, 16 parts of fulvic acid, ten
5 parts of dialkyl benzene sulfonic acids sodium.
(2) selenium rich of egg white matter is extracted：The above-mentioned light violet russian fenugreek herb of gained selenium-rich is rinsed well, shredded with cutting machine, then to
It adds the water of 3 times of volumes, is placed in beater and is beaten 20min, then squeezes out slurries with squeezer, then with double-layer filter cloth mistake
Filter pulp liquid, collect filtrate, then by filtrate at 55 DEG C heated at constant temperature 20min, be placed in a centrifuge after cooling with 4000 revs/min
Rotating speed centrifuges, and gained sediment is drying to obtain selenium rich of egg white matter；Selenium content is 6.0mg/kg after measured, and Protein Extraction is produced
Rate is 51%, lipidated protein 76%.
Embodiment 2：A kind of preparation method of feeding selenium rich of egg white matter, comprises the following steps：
(1) selenium-enriched alfalfa produces：In 2013 in the alfalfa life of Yellow River in Henan Province Tan Hebo grass cultivation Co., Ltd
Producing region, when Growth of Alfalfa is to 4~6cm of plant height, start, to micro- rich water solution that its spraying concentration is 240mg/kg, to spray
Measure as 45kg/667m2, when afternoon 5 after spray, sprayed once every 4 days, even spray 4 times, spray after such as rain, it is necessary to weigh
Spray, is gathered in, produces the light violet russian fenugreek herb of selenium-rich for the 5th day after the 4th sprays (Se content is 10.0mg/kg after measured)；
Above-mentioned micro- fertilizer is made up of the raw material of following parts by weight：15 parts of ammonium molybdate tetrahydrate, 22 parts of white vitriol, five water sulphur
Sour 17 parts of copper, 21 parts of ferrous sulfate heptahydrate, 15 parts of manganese sulfate, 20 parts of calcium dihydrogen phosphate, 100 parts of sodium selenite, 20 parts of fulvic acid,
10 parts of neopelex.
(2) selenium rich of egg white matter is extracted：The above-mentioned light violet russian fenugreek herb of gained selenium-rich is rinsed well, shredded with cutting machine, then to
It adds the water of 7 times of volumes, is placed in beater and is beaten 60min, then squeezes out slurries with squeezer, then with double-layer filter cloth mistake
Filter pulp liquid, collect filtrate, then by filtrate at 85 DEG C heated at constant temperature 60min, be placed in a centrifuge after cooling with 6000 revs/min
Rotating speed centrifuges, and gained sediment is drying to obtain selenium rich of egg white matter；Selenium content is 21.5mg/kg after measured, Protein Extraction
Yield is 63%, lipidated protein 68%.
Embodiment 3：A kind of preparation method of feeding selenium rich of egg white matter, comprises the following steps：
(1) selenium-enriched alfalfa produces：In 2012 in the alfalfa life of Yellow River in Henan Province Tan Hebo grass cultivation Co., Ltd
Producing region, when Growth of Alfalfa is to 4~6cm of plant height, start, to micro- rich water solution that its spraying concentration is 110mg/kg, to spray
Measure as 45kg/667m2, when afternoon 5 after spray, sprayed once every 4 days, even spray 4 times, spray after such as rain, it is necessary to weigh
Spray, is gathered in, produces the light violet russian fenugreek herb of selenium-rich for the 5th day after the 4th sprays (Se content is 1.6mg/kg after measured).
Micro- fertilizer is made up of the raw material of following parts by weight：10 parts of ammonium molybdate tetrahydrate, 17 parts of white vitriol, five water sulphur
Sour 12 parts of copper, 16 parts of ferrous sulfate heptahydrate, 9 parts of manganese sulfate, 15 parts of calcium dihydrogen phosphate, 10 parts of sodium selenite, 16 parts of fulvic acid, ten
5 parts of dialkyl benzene sulfonic acids sodium.
(2) selenium rich of egg white matter is extracted：The above-mentioned light violet russian fenugreek herb of gained selenium-rich is rinsed well, shredded with cutting machine, then to
It adds the water of 7 times of volumes, is placed in beater and is beaten 60min, then squeezes out slurries with squeezer, then with double-layer filter cloth mistake
Filter pulp liquid, collect filtrate, then by filtrate at 85 DEG C heated at constant temperature 60min, be placed in a centrifuge after cooling with 6000 revs/min
Rotating speed centrifuges, and gained sediment is drying to obtain selenium rich of egg white matter；Selenium content is 4.5mg/kg after measured, and Protein Extraction is produced
Rate is 63%, lipidated protein 68%.
Embodiment 4：A kind of preparation method of feeding selenium rich of egg white matter, comprises the following steps：
(1) selenium-enriched alfalfa produces：In 2013 in the alfalfa life of Yellow River in Henan Province Tan Hebo grass cultivation Co., Ltd
Producing region, when Growth of Alfalfa is to 4~6cm of plant height, start, to micro- rich water solution that its spraying concentration is 240mg/kg, to spray
Measure as 45kg/667m2, when afternoon 5 after spray, sprayed once every 4 days, even spray 4 times, spray after such as rain, it is necessary to weigh
Spray, is gathered in, produces the light violet russian fenugreek herb of selenium-rich for the 5th day after the 4th sprays (Se content is 10.0mg/kg after measured)；
Above-mentioned micro- fertilizer is made up of the raw material of following parts by weight：15 parts of ammonium molybdate tetrahydrate, 22 parts of white vitriol, five water sulphur
Sour 17 parts of copper, 21 parts of ferrous sulfate heptahydrate, 15 parts of manganese sulfate, 20 parts of calcium dihydrogen phosphate, 100 parts of sodium selenite, 20 parts of fulvic acid,
10 parts of neopelex.
(2) selenium rich of egg white matter is extracted：The above-mentioned light violet russian fenugreek herb of gained selenium-rich is rinsed well, shredded with cutting machine, then to
It adds the water of 3 times of volumes, is placed in beater and is beaten 20min, then squeezes out slurries with squeezer, then with double-layer filter cloth mistake
Filter pulp liquid, collect filtrate, then by filtrate at 55 DEG C heated at constant temperature 20min, be placed in a centrifuge after cooling with 4000 revs/min
Rotating speed centrifuges, and gained sediment is drying to obtain selenium rich of egg white matter；Selenium content is 30.0mg/kg after measured, Protein Extraction
Yield is 51%, lipidated protein 76%.
Embodiment 5：A kind of preparation method of feeding selenium rich of egg white matter, comprises the following steps：
(1) selenium-enriched alfalfa produces：In 2012 in the alfalfa life of Yellow River in Henan Province Tan Hebo grass cultivation Co., Ltd
Producing region, when Growth of Alfalfa is to 4~6cm of plant height, start, to micro- rich water solution that its spraying concentration is 110mg/kg, to spray
Measure as 45kg/667m2, when afternoon 5 after spray, sprayed once every 4 days, even spray 4 times, spray after such as rain, it is necessary to weigh
Spray, is gathered in, produces the light violet russian fenugreek herb of selenium-rich for the 5th day after the 4th sprays (Se content is 1.6mg/kg after measured).
Micro- fertilizer is made up of the raw material of following parts by weight：10 parts of ammonium molybdate tetrahydrate, 17 parts of white vitriol, five water sulphur
Sour 12 parts of copper, 16 parts of ferrous sulfate heptahydrate, 9 parts of manganese sulfate, 15 parts of calcium dihydrogen phosphate, 10 parts of sodium selenite, 16 parts of fulvic acid, ten
5 parts of dialkyl benzene sulfonic acids sodium.
(2) selenium rich of egg white matter is extracted：The above-mentioned light violet russian fenugreek herb of gained selenium-rich is rinsed well, shredded with cutting machine, then to
It adds the water of 5 times of volumes, is placed in beater and is beaten 40min, then squeezes out slurries with squeezer, then with double-layer filter cloth mistake
Filter pulp liquid, collect filtrate, then by filtrate at 70 DEG C heated at constant temperature 40min, be placed in a centrifuge after cooling with 5000 revs/min
Rotating speed centrifuges, and gained sediment is drying to obtain selenium rich of egg white matter；Selenium content is 5.0mg/kg after measured, and Protein Extraction is produced
Rate is 57%, lipidated protein 72%.
Embodiment 6：A kind of preparation method of feeding selenium rich of egg white matter, comprises the following steps：
(1) selenium-enriched alfalfa produces：In 2013 in the alfalfa life of Yellow River in Henan Province Tan Hebo grass cultivation Co., Ltd
Producing region, when Growth of Alfalfa is to 4~6cm of plant height, start, to micro- rich water solution that its spraying concentration is 240mg/kg, to spray
Measure as 45kg/667m2, when afternoon 5 after spray, sprayed once every 4 days, even spray 4 times, spray after such as rain, it is necessary to weigh
Spray, is gathered in, produces the light violet russian fenugreek herb of selenium-rich for the 5th day after the 4th sprays (Se content is 10.0mg/kg after measured)；
Above-mentioned micro- fertilizer is made up of the raw material of following parts by weight：15 parts of ammonium molybdate tetrahydrate, 22 parts of white vitriol, five water sulphur
Sour 17 parts of copper, 21 parts of ferrous sulfate heptahydrate, 15 parts of manganese sulfate, 20 parts of calcium dihydrogen phosphate, 100 parts of sodium selenite, 20 parts of fulvic acid,
10 parts of neopelex.
(2) selenium rich of egg white matter is extracted：The above-mentioned light violet russian fenugreek herb of gained selenium-rich is rinsed well, shredded with cutting machine, then to
It adds the water of 5 times of volumes, is placed in beater and is beaten 40min, then squeezes out slurries with squeezer, then with double-layer filter cloth mistake
Filter pulp liquid, collect filtrate, then by filtrate at 70 DEG C heated at constant temperature 40min, be placed in a centrifuge after cooling with 5000 revs/min
Rotating speed centrifuges, and gained sediment is drying to obtain selenium rich of egg white matter；Selenium content is 25.0mg/kg after measured, Protein Extraction
Yield is 57%, lipidated protein 72%.
2013 in Henan lotus milk industry development in science and technology Co., Ltd and Yellow River in Henan Province kind chicken house carry out selenium rich of egg respectively
White matter animal experiment, by Se content obtained above be 25mg/kg selenium rich of egg white matter in 1.5% ratio (percentage by weight)
It is added to mix in milk cow conventional feed and carries out feeding experiment, as a result show the feed for adding selenium rich of egg white matter, after feeding cow,
Milk cow daily yielding improves 8.2%, and material milk ratio reduces by 10.15%, and butterfat, lactoprotein, lactose and non-fat solid content in milk
Content is respectively increased 20.05%, 7.08%, 3.01% and 4.12%, improves milk quality, milk cow immunity is improved, also to milk
The safe efficient selenium-supply of ox.
The selenium rich of egg white matter that Se content obtained above is 4.5mg/kg is added in 10% ratio (percentage by weight)
Mixed into broiler chicken conventional feed, particle diameter is made and carries out feeding experiment for 3mm or so pellet, as a result shows addition selenium-rich
After the feed feeding dorking of protein, broiler chicken daily gain improves 24.3%, and feedstuff-meat ratio reduces by 20.7%, and improves the immune of broiler chicken
Function, reach the purpose of the safe efficient selenium-supply of broiler chicken.
Claims (1)
1. a kind of preparation method of feeding selenium rich of egg white matter, comprises the following steps：
(1) selenium-enriched alfalfa produces：Alfalfa is cultivated according to a conventional method, when Growth of Alfalfa is to 4~6cm of plant height,
Start to micro- rich water solution that its spraying concentration is 110~240mg/kg, the amount of spraying 45kg/667m2, when afternoon 5 after spray
Apply, sprayed once every 4 days, even spray 4 times, gathered within the 5th day after the 4th sprays such as rain, it is necessary to respray after spraying,
Produce the light violet russian fenugreek herb of selenium-rich；
Micro- fertilizer is made up of the raw material of following parts by weight：10~15 parts of ammonium molybdate tetrahydrate, 17~22 parts of white vitriol, five
12~17 parts of brochanite, 16~21 parts of ferrous sulfate heptahydrate, 9~15 parts of manganese sulfate, 15~20 parts of calcium dihydrogen phosphate, selenous acid
10~100 parts of sodium, 16~20 parts of fulvic acid, 5~10 parts of neopelex；
(2) selenium rich of egg white matter is extracted：The above-mentioned light violet russian fenugreek herb of gained selenium-rich is rinsed well, shredded with cutting machine, then add to it
Enter the water of 3~7 times of volumes, be placed in 20~60min of mashing in beater, then slurries are squeezed out with squeezer, then with double-deck filter
Cloth filter slurries, collect filtrate, then by filtrate at 55~85 DEG C 20~60min of heated at constant temperature, be placed in a centrifuge after cooling
Centrifuged with 4000~6000 revs/min of rotating speed, gained sediment is drying to obtain selenium rich of egg white matter.
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| 功能性植物蛋白源—苜蓿叶蛋白加热盐溶法浸提正交试验研究;于安芬等;《中国农学通报》;20110531;第27卷(第5期);457页引言部分,458页1.3试验方法 * |
| 喷施微肥对紫花苜蓿营养品质的影响;胡华锋等;《中国草地学报》;20070331;第29卷(第2期);第107页 * |
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