CN104543366A - Preparation method for forage-use selenium-rich protein - Google Patents

Preparation method for forage-use selenium-rich protein Download PDF

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CN104543366A
CN104543366A CN201410857899.9A CN201410857899A CN104543366A CN 104543366 A CN104543366 A CN 104543366A CN 201410857899 A CN201410857899 A CN 201410857899A CN 104543366 A CN104543366 A CN 104543366A
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selenium
rich
parts
protein
alfalfa
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CN104543366B (en
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胡华锋
介晓磊
刘桂珍
李见云
郭孝
王文静
董县中
李丽霞
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Henan University of Animal Husbandry and Economy
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Henan University of Animal Husbandry and Economy
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Abstract

The invention relates to a preparation method for forage-use selenium-rich protein. The preparation method comprises the following steps: spraying a micro fertilizer water solution onto alfalfa to prepare selenium-rich fresh alfalfa; conducting beating and squeezing on the selenium-rich fresh alfalfa to obtain a filtrate; heating the filtrate at a constant temperature ranging from 55 DEG C to 85 DEG C, cooling the heated filtrate, and then carrying out centrifugation to obtain a precipitate; drying the precipitate to obtain the forage-use selenium-rich protein. Through the adoption of the preparation method provided by the invention, the forage-use selenium-rich protein is natural and selenium-containing, and the selenium content of the forage-use selenium-rich protein can reach 4.5-30 mg/kg; the raw material cost is low, but the extraction yield and the purity of protein are relatively high, that is, the protein yield of the selenium-rich fresh alfalfa is 51-63%, and the protein purity reaches 68-76%; the forage-use selenium-rich protein is used for supplementing essential protein and selenium for livestock and poultry at the same time, so that the functions of nutrition and health care, immune enhancement, and improvement of the livestock and poultry productivity can be realized; the forage-use selenium-rich protein is free of hormones and side effects, nontoxic, and safe to use.

Description

The preparation method of feeding selenium rich of egg white matter
Technical field:
The present invention relates to a kind of preparation method of protein, particularly a kind of with the preparation method of the rich selenium alfalfa feeding selenium rich of egg white matter that is raw material.
Background technology:
Selenium (Se) is the required micronutrient element of animal, play an important role in animal body metabolism, scarce selenium can cause animal body inner cell antiperoxide toxicity ability to reduce, by peroxide breakdown, there is oedema, hemorrhage, exudative diathesis in cell, necrosis of liver cells, the atrophy of spleen fibroid, muscle is abnormal, muscular degeneration of heart; Show as white muscle disease, physiological disorders, growth retardation etc.Due to weather and soil, China has the soil of 2/3 to lack selenium, causes the shortage of selenium element in animal forage grass, therefore, needs to add selenium in animal feed, mainly in animal feed, directly adds mineral salt at present, as sodium selenite (Na 2seO 3), sodium selenate (Na 2seO 4or Na 2seO 410H 2o) or organic preparation, as selenomethionine and strengthening selenium yeast etc.Although inorganic selenium content high cost is low, requirement and dosis toxica differ very little, very easily poisoning, and in production, application security is poor; Although organic preparation utilizes safety than mineral salt, good absorbing, complex manufacturing, price is high.
Protein is the material base of life, does not have life without protein.If protein content is not enough in livestock and poultry diet, growth is by limited, and Body weight loss, efficiency of feed utilization reduces, and reproductive capability is disorderly.Therefore, for meeting the nutritional need that livestock and poultry normal growth is grown, often need in feed for livestock to add a certain amount of protein feeds, as beans, puffed soybean, fish meal etc., but the lipidated protein of these protein feedss is low, and addition precision prescribed is high.
Therefore, a kind of high-purity feed protein matter that is safe, that be rich in Organic Selenium is efficiently developed significant.
Alfalfa (Medicaco sativa L.) is a kind of high-quality feed resource, not only containing higher protein, and have very strong selenium rich ability, and in body, Enriching Selenium mainly exists with selenoprotein form.Utilize alfalfa to the high conversion of inorganic selenium and high protein characteristic, by mass transfer effect, inorganic selenium is converted into the activated selenoprotein of tool, then by thermal precipitation method, the selenium rich of egg white matter in rich selenium alfalfa is separated out.
At present, the pertinent literature report extracting selenium rich of egg white matter from rich selenium alfalfa is not also seen.
Summary of the invention:
The invention provides a kind of with the light violet russian fenugreek herb of rich selenium for the method for selenium rich of egg white matter prepared by raw material.Selenium Supplement while prepared selenium rich of egg white matter is used for supplementing livestock and poultry proteins necessary, has the function of nutrition and health care, develop immunitypty, raising Production of Livestock and Poultry power.
The present invention is achieved through the following technical solutions foregoing invention object:
Design a kind of feeding selenium rich of egg white matter preparation method, comprise the following steps:
(1) rich selenium alfalfa is produced: cultivate alfalfa according to a conventional method, and when Growth of Alfalfa is to plant height 4 ~ 6cm, starting is micro-rich water solution of 110 ~ 240mg/kg to its spraying concentration, and the amount of spraying is 45kg/667m 2, spray after in afternoon 5 time, sprayed once every 4 days, connect spray 4 times, as rain after spraying, need to respray, within the 5th day after the 4th sprays, gather in, obtain the light violet russian fenugreek herb of rich selenium;
Above-mentioned micro-fertilizer is made up of the raw material of following weight portion: four water ammonium molybdates 10 ~ 15 parts, white vitriol 17 ~ 22 parts, cupric sulfate pentahydrate 12 ~ 17 parts, ferrous sulfate heptahydrate 16 ~ 21 parts, manganese sulphate 9 ~ 15 parts, calcium dihydrogen phosphate 15 ~ 20 parts, sodium selenite 10 ~ 100 parts, fulvic acid 16 ~ 20 parts, neopelex 5 ~ 10 parts.
(2) selenium rich of egg white matter is extracted: rinsed well by the light violet russian fenugreek herb of the rich selenium of above-mentioned gained, shred with cutting machine, the water of 3 ~ 7 times of volumes is added again to it, be placed in beater making beating 20 ~ 60min, then squeeze out slurries with squeezer, then use double-layer filter cloth Filter slurry, collect filtrate, again by filtrate heated at constant temperature 20 ~ 60min at 55 ~ 85 DEG C, cooling is placed on the rotating speed centrifugation of 4000 ~ 6000 revs/min in centrifuge, and namely gained drying precipitate obtains selenium rich of egg white matter.
The present invention has following actively useful technique effect:
1. the selenium rich of egg white matter that the inventive method is prepared is the natural selenoprotein in rich selenium alfalfa, and wherein selenium is with the Organic Selenium of selenocysteine and derivative, selenomethionine and derivative thereof or certain intermolecular force and combined with protein (being mainly selenoprotein, containing selenium polypeptide, amino etc. containing selenium containing selenium material).Prepared selenium rich of egg white matter Se content can reach 4.5 ~ 30mg/kg.
2. the low and Protein Extraction productive rate of adopted cost of material and purity higher, the protein yields of the light violet russian fenugreek herb of rich selenium is 51 ~ 63%, and lipidated protein reaches 68 ~ 76%.
3., by selenium rich of egg white matter Selenium Supplement while supplementary livestock and poultry proteins necessary prepared by rich selenium alfalfa, there is effect of nutrition and health care, develop immunitypty, raising Production of Livestock and Poultry power, and without any hormone, nontoxic, have no side effect, use safety.
4. also can this selenium rich of egg white matter be added in animal and fowl fodder, produce selenium-rich livestock and fowls animal product, as selenium rich of egg, rich selenium poultry etc.
Embodiment:
Embodiment 1: a kind of preparation method of feeding selenium rich of egg white matter, comprises the steps:
(1) rich selenium alfalfa is produced: in 2012 in Yellow River in Henan Province Tan Hebo grass cultivation Co., Ltd's alfalfa production area, when Growth of Alfalfa is to plant height 4 ~ 6cm, beginning is micro-rich water solution of 110mg/kg to its spraying concentration, and the amount of spraying is 45kg/667m 2, spray after in afternoon 5 time, sprayed once every 4 days, connect spray 4 times, as rain after spraying, need to respray, within the 5th day after the 4th sprays, gather in, obtain the light violet russian fenugreek herb of rich selenium (Se content is 1.6mg/kg after measured).
Described micro-fertilizer is made up of the raw material of following weight portion: four water ammonium molybdates 10 parts, white vitriol 17 parts, cupric sulfate pentahydrate 12 parts, ferrous sulfate heptahydrate 16 parts, manganese sulphate 9 parts, calcium dihydrogen phosphate 15 parts, sodium selenite 10 parts, fulvic acid 16 parts, neopelex 5 parts.
(2) selenium rich of egg white matter is extracted: rinsed well by the light violet russian fenugreek herb of the rich selenium of above-mentioned gained, shred with cutting machine, the water of 3 times of volumes is added again to it, be placed in beater to pull an oar 20min, then squeeze out slurries with squeezer, then use double-layer filter cloth Filter slurry, collect filtrate, again by filtrate heated at constant temperature 20min at 55 DEG C, cooling is placed on the rotating speed centrifugation of 4000 revs/min in centrifuge, and namely gained drying precipitate obtains selenium rich of egg white matter; Selenium content is 6.0mg/kg after measured, and Protein Extraction productive rate is 51%, and lipidated protein is 76%.
Embodiment 2: a kind of preparation method of feeding selenium rich of egg white matter, comprises the steps:
(1) rich selenium alfalfa is produced: in 2013 in Yellow River in Henan Province Tan Hebo grass cultivation Co., Ltd's alfalfa production area, when Growth of Alfalfa is to plant height 4 ~ 6cm, beginning is micro-rich water solution of 240mg/kg to its spraying concentration, and the amount of spraying is 45kg/667m 2, spray after in afternoon 5 time, sprayed once every 4 days, connect spray 4 times, as rain after spraying, need to respray, within the 5th day after the 4th sprays, gather in, obtain the light violet russian fenugreek herb of rich selenium (Se content is 10.0mg/kg after measured);
Above-mentioned micro-fertilizer is made up of the raw material of following weight portion: four water ammonium molybdates 15 parts, white vitriol 22 parts, cupric sulfate pentahydrate 17 parts, ferrous sulfate heptahydrate 21 parts, manganese sulphate 15 parts, calcium dihydrogen phosphate 20 parts, sodium selenite 100 parts, fulvic acid 20 parts, neopelex 10 parts.
(2) selenium rich of egg white matter is extracted: rinsed well by the light violet russian fenugreek herb of the rich selenium of above-mentioned gained, shred with cutting machine, the water of 7 times of volumes is added again to it, be placed in beater to pull an oar 60min, then squeeze out slurries with squeezer, then use double-layer filter cloth Filter slurry, collect filtrate, again by filtrate heated at constant temperature 60min at 85 DEG C, cooling is placed on the rotating speed centrifugation of 6000 revs/min in centrifuge, and namely gained drying precipitate obtains selenium rich of egg white matter; Selenium content is 21.5mg/kg after measured, and Protein Extraction productive rate is 63%, and lipidated protein is 68%.
Embodiment 3: a kind of preparation method of feeding selenium rich of egg white matter, comprises the steps:
(1) rich selenium alfalfa is produced: in 2012 in Yellow River in Henan Province Tan Hebo grass cultivation Co., Ltd's alfalfa production area, when Growth of Alfalfa is to plant height 4 ~ 6cm, beginning is micro-rich water solution of 110mg/kg to its spraying concentration, and the amount of spraying is 45kg/667m 2, spray after in afternoon 5 time, sprayed once every 4 days, connect spray 4 times, as rain after spraying, need to respray, within the 5th day after the 4th sprays, gather in, obtain the light violet russian fenugreek herb of rich selenium (Se content is 1.6mg/kg after measured).
Described micro-fertilizer is made up of the raw material of following weight portion: four water ammonium molybdates 10 parts, white vitriol 17 parts, cupric sulfate pentahydrate 12 parts, ferrous sulfate heptahydrate 16 parts, manganese sulphate 9 parts, calcium dihydrogen phosphate 15 parts, sodium selenite 10 parts, fulvic acid 16 parts, neopelex 5 parts.
(2) selenium rich of egg white matter is extracted: rinsed well by the light violet russian fenugreek herb of the rich selenium of above-mentioned gained, shred with cutting machine, the water of 7 times of volumes is added again to it, be placed in beater to pull an oar 60min, then squeeze out slurries with squeezer, then use double-layer filter cloth Filter slurry, collect filtrate, again by filtrate heated at constant temperature 60min at 85 DEG C, cooling is placed on the rotating speed centrifugation of 6000 revs/min in centrifuge, and namely gained drying precipitate obtains selenium rich of egg white matter; Selenium content is 4.5mg/kg after measured, and Protein Extraction productive rate is 63%, and lipidated protein is 68%.
Embodiment 4: a kind of preparation method of feeding selenium rich of egg white matter, comprises the steps:
(1) rich selenium alfalfa is produced: in 2013 in Yellow River in Henan Province Tan Hebo grass cultivation Co., Ltd's alfalfa production area, when Growth of Alfalfa is to plant height 4 ~ 6cm, beginning is micro-rich water solution of 240mg/kg to its spraying concentration, and the amount of spraying is 45kg/667m 2, spray after in afternoon 5 time, sprayed once every 4 days, connect spray 4 times, as rain after spraying, need to respray, within the 5th day after the 4th sprays, gather in, obtain the light violet russian fenugreek herb of rich selenium (Se content is 10.0mg/kg after measured);
Above-mentioned micro-fertilizer is made up of the raw material of following weight portion: four water ammonium molybdates 15 parts, white vitriol 22 parts, cupric sulfate pentahydrate 17 parts, ferrous sulfate heptahydrate 21 parts, manganese sulphate 15 parts, calcium dihydrogen phosphate 20 parts, sodium selenite 100 parts, fulvic acid 20 parts, neopelex 10 parts.
(2) selenium rich of egg white matter is extracted: rinsed well by the light violet russian fenugreek herb of the rich selenium of above-mentioned gained, shred with cutting machine, the water of 3 times of volumes is added again to it, be placed in beater to pull an oar 20min, then squeeze out slurries with squeezer, then use double-layer filter cloth Filter slurry, collect filtrate, again by filtrate heated at constant temperature 20min at 55 DEG C, cooling is placed on the rotating speed centrifugation of 4000 revs/min in centrifuge, and namely gained drying precipitate obtains selenium rich of egg white matter; Selenium content is 30.0mg/kg after measured, and Protein Extraction productive rate is 51%, and lipidated protein is 76%.
Embodiment 5: a kind of preparation method of feeding selenium rich of egg white matter, comprises the steps:
(1) rich selenium alfalfa is produced: in 2012 in Yellow River in Henan Province Tan Hebo grass cultivation Co., Ltd's alfalfa production area, when Growth of Alfalfa is to plant height 4 ~ 6cm, beginning is micro-rich water solution of 110mg/kg to its spraying concentration, and the amount of spraying is 45kg/667m 2, spray after in afternoon 5 time, sprayed once every 4 days, connect spray 4 times, as rain after spraying, need to respray, within the 5th day after the 4th sprays, gather in, obtain the light violet russian fenugreek herb of rich selenium (Se content is 1.6mg/kg after measured).
Described micro-fertilizer is made up of the raw material of following weight portion: four water ammonium molybdates 10 parts, white vitriol 17 parts, cupric sulfate pentahydrate 12 parts, ferrous sulfate heptahydrate 16 parts, manganese sulphate 9 parts, calcium dihydrogen phosphate 15 parts, sodium selenite 10 parts, fulvic acid 16 parts, neopelex 5 parts.
(2) selenium rich of egg white matter is extracted: rinsed well by the light violet russian fenugreek herb of the rich selenium of above-mentioned gained, shred with cutting machine, the water of 5 times of volumes is added again to it, be placed in beater to pull an oar 40min, then squeeze out slurries with squeezer, then use double-layer filter cloth Filter slurry, collect filtrate, again by filtrate heated at constant temperature 40min at 70 DEG C, cooling is placed on the rotating speed centrifugation of 5000 revs/min in centrifuge, and namely gained drying precipitate obtains selenium rich of egg white matter; Selenium content is 5.0mg/kg after measured, and Protein Extraction productive rate is 57%, and lipidated protein is 72%.
Embodiment 6: a kind of preparation method of feeding selenium rich of egg white matter, comprises the steps:
(1) rich selenium alfalfa is produced: in 2013 in Yellow River in Henan Province Tan Hebo grass cultivation Co., Ltd's alfalfa production area, when Growth of Alfalfa is to plant height 4 ~ 6cm, beginning is micro-rich water solution of 240mg/kg to its spraying concentration, and the amount of spraying is 45kg/667m 2, spray after in afternoon 5 time, sprayed once every 4 days, connect spray 4 times, as rain after spraying, need to respray, within the 5th day after the 4th sprays, gather in, obtain the light violet russian fenugreek herb of rich selenium (Se content is 10.0mg/kg after measured);
Above-mentioned micro-fertilizer is made up of the raw material of following weight portion: four water ammonium molybdates 15 parts, white vitriol 22 parts, cupric sulfate pentahydrate 17 parts, ferrous sulfate heptahydrate 21 parts, manganese sulphate 15 parts, calcium dihydrogen phosphate 20 parts, sodium selenite 100 parts, fulvic acid 20 parts, neopelex 10 parts.
(2) selenium rich of egg white matter is extracted: rinsed well by the light violet russian fenugreek herb of the rich selenium of above-mentioned gained, shred with cutting machine, the water of 5 times of volumes is added again to it, be placed in beater to pull an oar 40min, then squeeze out slurries with squeezer, then use double-layer filter cloth Filter slurry, collect filtrate, again by filtrate heated at constant temperature 40min at 70 DEG C, cooling is placed on the rotating speed centrifugation of 5000 revs/min in centrifuge, and namely gained drying precipitate obtains selenium rich of egg white matter; Selenium content is 25.0mg/kg after measured, and Protein Extraction productive rate is 57%, and lipidated protein is 72%.
Within 2013, carry out selenium rich of egg white matter animal experiment respectively in Henan Zhong Henaiye development in science and technology Co., Ltd and Yellow River in Henan Province kind chicken house, by above-mentioned obtained Se content be 25mg/kg selenium rich of egg white matter in 1.5% ratio (percentage by weight) to add in milk cow conventional feed mixing to and carry out feeding experiment, the feed of selenium rich of egg white matter is added in result display, after feeding cow, milk cow daily yielding improves 8.2%, material milk is than reduction by 10.15%, and butterfat in milk, milk protein, lactose and non-fat solid content improve 20.05% respectively, 7.08%, 3.01% and 4.12%, improve milk quality, improve milk cow immunity, also to milk cow safety, efficient benefit selenium.
By above-mentioned obtained Se content be 4.5mg/kg selenium rich of egg white matter in 10% ratio (percentage by weight) add in broiler chicken conventional feed and mix, making particle diameter is that the pellet of about 3mm carries out feeding experiment, after the feed feeding dorking of selenium rich of egg white matter is added in result display, broiler chicken daily gain improves 24.3%, feedstuff-meat ratio reduces by 20.7%, and improve the immunologic function of broiler chicken, reach broiler chicken safety, efficient object of mending selenium.

Claims (1)

1. a preparation method for feeding selenium rich of egg white matter, comprises the following steps:
(1) rich selenium alfalfa is produced: cultivate alfalfa according to a conventional method, and when Growth of Alfalfa is to plant height 4 ~ 6cm, starting is micro-rich water solution of 110 ~ 240mg/kg to its spraying concentration, the amount of spraying 45kg/667m 2, spray after in afternoon 5 time, sprayed once every 4 days, connect spray 4 times, as rain after spraying, need to respray, within the 5th day after the 4th sprays, gather in, obtain the light violet russian fenugreek herb of rich selenium;
Described micro-fertilizer is made up of the raw material of following weight portion: four water ammonium molybdates 10 ~ 15 parts, white vitriol 17 ~ 22 parts, cupric sulfate pentahydrate 12 ~ 17 parts, ferrous sulfate heptahydrate 16 ~ 21 parts, manganese sulphate 9 ~ 15 parts, calcium dihydrogen phosphate 15 ~ 20 parts, sodium selenite 10 ~ 100 parts, fulvic acid 16 ~ 20 parts, neopelex 5 ~ 10 parts;
(2) selenium rich of egg white matter is extracted: rinsed well by the light violet russian fenugreek herb of the rich selenium of above-mentioned gained, shred with cutting machine, the water of 3 ~ 7 times of volumes is added again to it, be placed in beater making beating 20 ~ 60min, then squeeze out slurries with squeezer, then use double-layer filter cloth Filter slurry, collect filtrate, again by filtrate heated at constant temperature 20 ~ 60min at 55 ~ 85 DEG C, cooling is placed on the rotating speed centrifugation of 4000 ~ 6000 revs/min in centrifuge, and namely gained drying precipitate obtains selenium rich of egg white matter.
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Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN107251826A (en) * 2017-06-26 2017-10-17 徐州市贾汪区堡垒种植家庭农场 A kind of insect prevention culture matrix for culturing vegetable
CN107683946A (en) * 2017-09-10 2018-02-13 湖南七纬科技有限公司 A kind of selenium-rich habit-breaking candy for smokers and preparation method thereof
CN109418461A (en) * 2017-09-04 2019-03-05 王琳 A kind of office health preserving tea drink formula

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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN107251826A (en) * 2017-06-26 2017-10-17 徐州市贾汪区堡垒种植家庭农场 A kind of insect prevention culture matrix for culturing vegetable
CN109418461A (en) * 2017-09-04 2019-03-05 王琳 A kind of office health preserving tea drink formula
CN107683946A (en) * 2017-09-10 2018-02-13 湖南七纬科技有限公司 A kind of selenium-rich habit-breaking candy for smokers and preparation method thereof

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