CN104531526B - 一种磁珠法核酸提取装置 - Google Patents
一种磁珠法核酸提取装置 Download PDFInfo
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- CN104531526B CN104531526B CN201510017498.7A CN201510017498A CN104531526B CN 104531526 B CN104531526 B CN 104531526B CN 201510017498 A CN201510017498 A CN 201510017498A CN 104531526 B CN104531526 B CN 104531526B
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- magnet
- nucleic acid
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- jack
- extracting apparatus
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
- C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
- C12N15/1013—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers by using magnetic beads
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M47/00—Means for after-treatment of the produced biomass or of the fermentation or metabolic products, e.g. storage of biomass
- C12M47/06—Hydrolysis; Cell lysis; Extraction of intracellular or cell wall material
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B03—SEPARATION OF SOLID MATERIALS USING LIQUIDS OR USING PNEUMATIC TABLES OR JIGS; MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
- B03C—MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
- B03C1/00—Magnetic separation
- B03C1/02—Magnetic separation acting directly on the substance being separated
- B03C1/04—Magnetic separation acting directly on the substance being separated with the material carriers in the form of trays or with tables
- B03C1/06—Magnetic separation acting directly on the substance being separated with the material carriers in the form of trays or with tables with magnets moving during operation
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B03—SEPARATION OF SOLID MATERIALS USING LIQUIDS OR USING PNEUMATIC TABLES OR JIGS; MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
- B03C—MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
- B03C2201/00—Details of magnetic or electrostatic separation
- B03C2201/18—Magnetic separation whereby the particles are suspended in a liquid
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- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Genetics & Genomics (AREA)
- Biomedical Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Organic Chemistry (AREA)
- Biotechnology (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Molecular Biology (AREA)
- Microbiology (AREA)
- Analytical Chemistry (AREA)
- Crystallography & Structural Chemistry (AREA)
- Physics & Mathematics (AREA)
- Biophysics (AREA)
- Plant Pathology (AREA)
- Sustainable Development (AREA)
- Cell Biology (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
Description
Claims (10)
Priority Applications (4)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201510017498.7A CN104531526B (zh) | 2015-01-14 | 2015-01-14 | 一种磁珠法核酸提取装置 |
PCT/CN2015/087791 WO2016112694A1 (zh) | 2015-01-14 | 2015-08-21 | 一种磁珠法核酸提取装置 |
EP15877607.0A EP3222710B1 (en) | 2015-01-14 | 2015-08-21 | Device for nucleic acid extraction using magnetic bead method |
US15/622,673 US9896684B2 (en) | 2015-01-14 | 2017-06-14 | Device for nucleic acid extraction using magnetic bead method |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201510017498.7A CN104531526B (zh) | 2015-01-14 | 2015-01-14 | 一种磁珠法核酸提取装置 |
Publications (2)
Publication Number | Publication Date |
---|---|
CN104531526A CN104531526A (zh) | 2015-04-22 |
CN104531526B true CN104531526B (zh) | 2016-08-24 |
Family
ID=52847162
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CN201510017498.7A Active CN104531526B (zh) | 2015-01-14 | 2015-01-14 | 一种磁珠法核酸提取装置 |
Country Status (4)
Country | Link |
---|---|
US (1) | US9896684B2 (zh) |
EP (1) | EP3222710B1 (zh) |
CN (1) | CN104531526B (zh) |
WO (1) | WO2016112694A1 (zh) |
Families Citing this family (14)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN104531526B (zh) * | 2015-01-14 | 2016-08-24 | 湖南圣维基因科技有限公司 | 一种磁珠法核酸提取装置 |
US20200363299A1 (en) * | 2016-11-23 | 2020-11-19 | Hangzhou Gene-Meta Medical Device Co., Ltd. | Apparatus for automating pretreatment of nucleic acid detection |
BR112020001252B1 (pt) * | 2017-07-21 | 2023-03-21 | Seegene, Inc | Módulos para transferir beads magnéticas, sistema automatizado compreendendo as mesmas e método para extração de ácidos nucleicos usando as mesmas |
CN108795699B (zh) * | 2018-07-19 | 2023-12-26 | 嘉兴医脉赛科技有限公司 | 封闭式磁珠纯化仪 |
CN110408519A (zh) * | 2019-09-04 | 2019-11-05 | 苏州达微致和基因科技有限公司 | 一种用于分子诊断的核酸提取装置 |
CN111218398B (zh) * | 2020-03-25 | 2024-02-13 | 广州高盛生物科技股份有限公司 | 一种磁珠提取模块及提取方法 |
DE102020209001B4 (de) * | 2020-07-17 | 2024-04-18 | Hahn-Schickard-Gesellschaft für angewandte Forschung e.V. | Lyse einer Probe mittels Magnetelementen und rotatorischer Relativbewegung |
CN112831496B (zh) * | 2021-02-24 | 2024-01-23 | 上海金诺奥美生物科技有限公司 | 核酸提取设备、控制方法及核酸提取系统 |
CN112852625A (zh) * | 2021-02-24 | 2021-05-28 | 珠海启奥生物技术有限公司 | 核酸提取用的试剂管组件及其包装组件 |
CN113083500B (zh) * | 2021-03-25 | 2022-07-22 | 甄融生物科技(上海)有限公司 | 一种磁珠纯化设备的控制方法 |
CN113583807A (zh) * | 2021-08-25 | 2021-11-02 | 无锡正则精准医学检验有限公司 | 一种大肠癌检测用核酸提取仪 |
USD1023334S1 (en) * | 2022-02-21 | 2024-04-16 | Sansure Biotech Inc. | Automatic nucleic acid detection analyzer |
CN114958571B (zh) * | 2022-05-23 | 2023-11-21 | 博迈德生物科技(固安)有限公司 | 一种自动化磁珠加样装置 |
CN114774241B (zh) * | 2022-06-22 | 2022-09-20 | 翊新诊断技术(苏州)有限公司 | 一种磁珠提取装置 |
Citations (5)
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US20070243600A1 (en) * | 2005-03-10 | 2007-10-18 | Gen-Probe Incorporated | System for performing multi-formatted assays |
CN102093952A (zh) * | 2010-11-24 | 2011-06-15 | 清华大学 | 基于电磁驱动研磨和离心分离的生物组分自动化提取装置 |
CN202075285U (zh) * | 2011-04-02 | 2011-12-14 | 合肥运涛光电科技有限公司 | 一种全盘加样微型转盘式全自动免疫发光分析系统 |
CN102427885A (zh) * | 2009-05-15 | 2012-04-25 | 简·探针公司 | 用于在执行磁性分离工序的仪器中实现磁体的自动移动的方法和设备 |
CN203360441U (zh) * | 2013-04-03 | 2013-12-25 | 上海睿珉机电科技有限公司 | 细胞分析仪中新型圆盘式细胞样品测量盘的底片结构 |
Family Cites Families (11)
Publication number | Priority date | Publication date | Assignee | Title |
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DE19512368A1 (de) * | 1995-04-01 | 1996-10-02 | Boehringer Mannheim Gmbh | System zur Freisetzung und Isolierung von Nukleinsäuren |
WO2007106013A1 (en) * | 2006-03-13 | 2007-09-20 | Gyros Patent Ab | Enhanced magnetic particle steering |
TWI296713B (en) * | 2006-08-02 | 2008-05-11 | Ind Tech Res Inst | Magnetic beads-based sample separating device |
JP2008167722A (ja) * | 2007-01-15 | 2008-07-24 | Konica Minolta Medical & Graphic Inc | 磁性支持体上での加熱による核酸単離方法 |
JP2011516075A (ja) * | 2008-04-09 | 2011-05-26 | バイオニア コーポレーション | 自動精製装置、マルチウェルプレートキット及び生物学的試料からヘキサンを抽出する方法 |
CN101665785B (zh) * | 2009-09-24 | 2011-02-16 | 戴立忠 | 一种采用磁珠从样品中提取并纯化核酸的方法 |
WO2013126620A1 (en) * | 2012-02-23 | 2013-08-29 | Dow Agrosciences Llc | Automation of barrier-based plant nucleic acid and protein extraction |
CN203668357U (zh) * | 2013-10-15 | 2014-06-25 | 周国华 | 集核酸提取、扩增与检测一体化的装置 |
CN103897987B (zh) * | 2014-02-18 | 2016-05-04 | 中国农业大学 | 基于纳米磁珠的核酸自动提取装置及其方法 |
CN103908945B (zh) * | 2014-03-31 | 2015-09-16 | 洛阳惠尔纳米科技有限公司 | 一种核酸提取磁珠的制备方法及应用 |
CN104531526B (zh) * | 2015-01-14 | 2016-08-24 | 湖南圣维基因科技有限公司 | 一种磁珠法核酸提取装置 |
-
2015
- 2015-01-14 CN CN201510017498.7A patent/CN104531526B/zh active Active
- 2015-08-21 EP EP15877607.0A patent/EP3222710B1/en active Active
- 2015-08-21 WO PCT/CN2015/087791 patent/WO2016112694A1/zh active Application Filing
-
2017
- 2017-06-14 US US15/622,673 patent/US9896684B2/en active Active
Patent Citations (5)
Publication number | Priority date | Publication date | Assignee | Title |
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US20070243600A1 (en) * | 2005-03-10 | 2007-10-18 | Gen-Probe Incorporated | System for performing multi-formatted assays |
CN102427885A (zh) * | 2009-05-15 | 2012-04-25 | 简·探针公司 | 用于在执行磁性分离工序的仪器中实现磁体的自动移动的方法和设备 |
CN102093952A (zh) * | 2010-11-24 | 2011-06-15 | 清华大学 | 基于电磁驱动研磨和离心分离的生物组分自动化提取装置 |
CN202075285U (zh) * | 2011-04-02 | 2011-12-14 | 合肥运涛光电科技有限公司 | 一种全盘加样微型转盘式全自动免疫发光分析系统 |
CN203360441U (zh) * | 2013-04-03 | 2013-12-25 | 上海睿珉机电科技有限公司 | 细胞分析仪中新型圆盘式细胞样品测量盘的底片结构 |
Non-Patent Citations (1)
Title |
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磁珠法核算自动提取仪在分子生物学领域的应用;罗英;《蚕学通讯》;20130630;第33卷(第2期);全文 * |
Also Published As
Publication number | Publication date |
---|---|
EP3222710A4 (en) | 2018-02-21 |
US9896684B2 (en) | 2018-02-20 |
CN104531526A (zh) | 2015-04-22 |
EP3222710B1 (en) | 2018-10-24 |
US20170275614A1 (en) | 2017-09-28 |
WO2016112694A1 (zh) | 2016-07-21 |
EP3222710A1 (en) | 2017-09-27 |
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PB01 | Publication | ||
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PE01 | Entry into force of the registration of the contract for pledge of patent right |
Denomination of invention: Device for extracting nucleic acid through magnetic bead method Effective date of registration: 20170711 Granted publication date: 20160824 Pledgee: Ningbo free trade zone Terry with equity investment partnership (limited partnership)|Suzhou equity equity investment center (limited partnership)|Ningbo Meishan Bonded Port District, Jun and equity investment partnership (limited partnership)|Chen Bang Pledgor: Hunan Gene Technology Co.|Hunan San Xiang Biological Technology Co. Ltd. Registration number: 2017430000042 |
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PE01 | Entry into force of the registration of the contract for pledge of patent right | ||
PC01 | Cancellation of the registration of the contract for pledge of patent right |
Date of cancellation: 20200217 Granted publication date: 20160824 Pledgee: Suzhou Lirui equity investment center (limited partnership)|Triton equity investment partnership (limited partnership)|JUNHE Tongrui equity investment partnership (limited partnership)|Chenbang Pledgor: SANWAY GENE TECH Inc. Registration number: 2017430000042 |