CN104528942A - Method for microorganism and aquatic plant cooperative purification of polluted water body - Google Patents
Method for microorganism and aquatic plant cooperative purification of polluted water body Download PDFInfo
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- CN104528942A CN104528942A CN201410710248.7A CN201410710248A CN104528942A CN 104528942 A CN104528942 A CN 104528942A CN 201410710248 A CN201410710248 A CN 201410710248A CN 104528942 A CN104528942 A CN 104528942A
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- 244000005700 microbiome Species 0.000 title claims abstract description 140
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 title claims abstract description 66
- 238000000746 purification Methods 0.000 title claims abstract description 29
- 238000000034 method Methods 0.000 title claims abstract description 28
- 238000002360 preparation method Methods 0.000 claims abstract description 16
- 230000012010 growth Effects 0.000 claims abstract description 6
- 241000196324 Embryophyta Species 0.000 claims description 58
- 230000000813 microbial effect Effects 0.000 claims description 20
- 230000002195 synergetic effect Effects 0.000 claims description 20
- 241000209094 Oryza Species 0.000 claims description 4
- 235000007164 Oryza sativa Nutrition 0.000 claims description 4
- 235000009566 rice Nutrition 0.000 claims description 4
- 241000894006 Bacteria Species 0.000 abstract description 11
- 150000001875 compounds Chemical class 0.000 abstract 2
- 230000035755 proliferation Effects 0.000 abstract 1
- 238000005406 washing Methods 0.000 abstract 1
- 238000004065 wastewater treatment Methods 0.000 abstract 1
- 230000000694 effects Effects 0.000 description 8
- 241000194108 Bacillus licheniformis Species 0.000 description 6
- 241000589776 Pseudomonas putida Species 0.000 description 6
- 241000589614 Pseudomonas stutzeri Species 0.000 description 6
- 239000004382 Amylase Substances 0.000 description 4
- 102000013142 Amylases Human genes 0.000 description 4
- 108010065511 Amylases Proteins 0.000 description 4
- 108010059892 Cellulase Proteins 0.000 description 4
- 102000035195 Peptidases Human genes 0.000 description 4
- 108091005804 Peptidases Proteins 0.000 description 4
- 235000019418 amylase Nutrition 0.000 description 4
- 229940106157 cellulase Drugs 0.000 description 4
- 239000003643 water by type Substances 0.000 description 4
- XKMRRTOUMJRJIA-UHFFFAOYSA-N ammonia nh3 Chemical compound N.N XKMRRTOUMJRJIA-UHFFFAOYSA-N 0.000 description 3
- 238000005516 engineering process Methods 0.000 description 3
- 230000007613 environmental effect Effects 0.000 description 3
- 230000008635 plant growth Effects 0.000 description 3
- QGZKDVFQNNGYKY-UHFFFAOYSA-N Ammonia Chemical compound N QGZKDVFQNNGYKY-UHFFFAOYSA-N 0.000 description 2
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 2
- 108090000790 Enzymes Proteins 0.000 description 2
- 102000004190 Enzymes Human genes 0.000 description 2
- 230000015556 catabolic process Effects 0.000 description 2
- 230000003247 decreasing effect Effects 0.000 description 2
- 238000006731 degradation reaction Methods 0.000 description 2
- 201000010099 disease Diseases 0.000 description 2
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 2
- 229940088598 enzyme Drugs 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
- 239000000203 mixture Substances 0.000 description 2
- 239000012629 purifying agent Substances 0.000 description 2
- 241000251468 Actinopterygii Species 0.000 description 1
- IOVCWXUNBOPUCH-UHFFFAOYSA-M Nitrite anion Chemical compound [O-]N=O IOVCWXUNBOPUCH-UHFFFAOYSA-M 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 229910021529 ammonia Inorganic materials 0.000 description 1
- 244000052616 bacterial pathogen Species 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000009395 breeding Methods 0.000 description 1
- 230000001488 breeding effect Effects 0.000 description 1
- 239000002131 composite material Substances 0.000 description 1
- 238000011109 contamination Methods 0.000 description 1
- 238000005202 decontamination Methods 0.000 description 1
- 230000003588 decontaminative effect Effects 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 238000012851 eutrophication Methods 0.000 description 1
- 238000011010 flushing procedure Methods 0.000 description 1
- 238000002513 implantation Methods 0.000 description 1
- 238000011835 investigation Methods 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 229910052757 nitrogen Inorganic materials 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 230000035764 nutrition Effects 0.000 description 1
- 230000000050 nutritive effect Effects 0.000 description 1
- 239000006041 probiotic Substances 0.000 description 1
- 235000018291 probiotics Nutrition 0.000 description 1
- 238000005067 remediation Methods 0.000 description 1
- 239000010865 sewage Substances 0.000 description 1
- 208000024891 symptom Diseases 0.000 description 1
- 239000002351 wastewater Substances 0.000 description 1
- 238000003911 water pollution Methods 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/32—Biological treatment of water, waste water, or sewage characterised by the animals or plants used, e.g. algae
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
- C02F3/342—Biological treatment of water, waste water, or sewage characterised by the microorganisms used characterised by the enzymes used
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W10/00—Technologies for wastewater treatment
- Y02W10/10—Biological treatment of water, waste water, or sewage
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- Life Sciences & Earth Sciences (AREA)
- Microbiology (AREA)
- Engineering & Computer Science (AREA)
- Water Supply & Treatment (AREA)
- Hydrology & Water Resources (AREA)
- Environmental & Geological Engineering (AREA)
- Biodiversity & Conservation Biology (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Biotechnology (AREA)
- Botany (AREA)
- Purification Treatments By Anaerobic Or Anaerobic And Aerobic Bacteria Or Animals (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention relates to the technical field of polluted water body improvement, and discloses a method for microorganism and aquatic plant cooperative purification of a polluted water body; the method comprises the following steps: A, the tube interior of a microorganism slow-release tube is filled with a compound microorganism preparation; B, the microorganism slow-release tube is applied in the polluted water body or bottom mud by aquatic plants; and C, along with the growth of the aquatic plants, microorganisms in the microorganism slow-release tube are gradually released and are attached to submerged plants, and a biofilm is formed. The microorganism and aquatic plant cooperative purification of the polluted water body is adopted. The microorganism slow-release tube filled with the compound microorganism preparation is adopted, on one hand, stability and washing resistance are greatly improved, on the other hand, the load capacity of microorganism bacteria and the proliferation of the microorganism bacteria are improved, and in general, the purpose of increasing the efficiency of wastewater treatment is achieved.
Description
Technical field
The present invention relates to the technical field improving polluted-water, particularly relate to a kind of method of microorganism and waterplant synergistic purification polluted-water.
Background technology
Along with the develop rapidly of China's economy, China's water environmental problems becomes increasingly conspicuous, and town and country water surrounding suffers from unprecedented threat.Existing investigation shows that Yangtze River Delta Region Rural Water Environment ecology more than 90% loses basic function, river course, town and country is because polluting and ecosystem serious degradation, river water quality is in V class and bad V class water quality substantially, most river water body is seriously polluted, eutrophication, and topmost contamination index is permanganate indices (COD
mn) and ammonia nitrogen, the most blackening fouling of river in addition, pathogenic bacteria exceed standard, and cause severe Environmental situations, have had a strong impact on the ecology in vast rural area, production and living safety.Thus be major issue anxious to be resolved to the reparation of town and country water surrounding.
At present, environmental improvement method is the most extensive with the application and research of microorganism remediation method, the advantages such as expense is low, treatment effect good because these class methods have, non-secondary pollution.
Microbial water-purifying agent for nutrition source, reaches the object of decreasing pollution with the rich nutritive material in water body.But no matter this series products is tablet form, powder-type or liquid-type has very large limitation, namely they can only close and the depth of water be no more than 2 meters waters use, run into flowing waters or lake cage culture is just helpless, because probiotics can dilute by current, dominant bacteria cannot be formed to reach the object of decreasing pollution.Therefore, this being greatly limited property of new technology effect, useful viable bacteria microbial water-purifying agent, since appearance is over 5 years, cannot use always in rivers and lakes cage culture with the water pollution control in flowing waters.
In addition, some prior art products, is adsorbed on the surface of carrier by chemically crosslinked by microbial bacteria completely, and cost of manufacture is large, and slow release effect is poor, can only be used for the small size pond such as aquarium fish or landscape water and use, and is not suitable for big area flowing waters.
Summary of the invention
The present invention is directed to the shortcoming of prior art, disclose a kind of method of microorganism and waterplant synergistic purification polluted-water.
In order to solve the problems of the technologies described above, the present invention is solved by following technical proposals.
The method of microorganism and waterplant synergistic purification polluted-water, comprises the following steps,
A. choose the microorganism slow release tube that caliber is 16mm, length is 10-20cm, microorganism slow release tube two seals, and pipe shaft spiles, and fills 0.05-0.1g g complex microorganism preparations in microorganism slow release tube pipe;
B. on the root of waterplant or stem, bundle a microorganism slow release tube, be that in the bed mud of 0.5-2 rice, plantation binding has the waterplant of microorganism slow release tube and/or plantation in plant plankton framework in the depth of water, and microorganism slow release tube is in bed mud or in water;
C. along with the growth of waterplant, the microorganism in microorganism slow release tube pipe progressively discharges, and is attached on submerged plant, forms microbial film.
As preferably, microorganism slow release tube is pvc pipe.
As preferably, waterplant is submerged plant or plant plankton.
As preferably, in step B, a binding microorganism slow release tube on the root of every strain submerged plant or stem, be in the bed mud of 0.5-2 rice in the depth of water, plantation binding has the submerged plant of microorganism slow release tube.
As preferably, plant plankton chooses the microorganism slow release tube that length is 10cm.
As preferably, in step B, 10 strain plant planktons are divided into one clump, tie up microorganism slow release tube in the middle of one clump of plant plankton, then planted in plant plankton framework.
As preferably, submerged plant chooses the microorganism slow release tube that length is 20cm.
The method of microorganism and waterplant synergistic purification polluted-water
Compared with prior art, beneficial effect of the present invention is:
(1) the present invention adopts microorganism and waterplant synergistic purification polluted-water.On the one hand, waterplant is that the implantation of microorganism water purification bacterium or flora provides good environment.On the other hand, when microorganism water purification slow release body is positioned in water, be attached to the microorganism water purification bacterium on waterplant or flora resurrection, and start breeding, be attached on submerged plant and form microbial film, the ammonia and nitrogen organic progressively in absorption water and nitrite etc., reach the object of water purification.Again on the one hand, adopt submerged plant and plant plankton simultaneously, work along both lines, act on water surface and deepwater regions simultaneously, better to the decontamination effect improving of polluted-water.
(2) using the carrier of microorganism slow release tube as complex microorganism preparations, microorganism slow release tube two seals, and pipe shaft spiles, and makes microbial preparation have preferably slow-release function.
(3) strong than general carrier of microorganism slow release tube bonding force, therefore microbial bacteria can not come off easily under water impact, while the viable bacteria fast onset water purification effect of release, also diluted by water or break up, therefore also can not damage or other detrimentally affects the original ecotope of water body.
(4) the present invention utilizes the method that high-effective microorganism and prozyme combine, waste water control instant effect, and nitric efficiency is high, really reaches the effect for the treatment of both principal and secondary aspect of disease.Use method of the present invention to administer town and country polluted river channel water body, after one week, the visibility meter of polluted-water brings up to 80-90cm from 30-40cm, and ammonia nitrogen degradation rate reaches more than 99%.Through the environment measuring of 3-5 month, within finding that ammonia nitrogen maintains III class water quality always, water purification water quality was better.Method of the present invention breaches curing the symptoms, not the disease of prior art, and once drop into after administering and can maintain the eubiosis for many years, not only purifying water effect is obvious, and water quality detection index is stablized, do not rebounded, and reduces running cost.
(5) the present invention adopts the microorganism slow release tube of filled composite microbial preparation, substantially increase stability, resistance to flushing performance on the one hand, also improve the charge capacity of microbial bacteria and the propagation of microbial bacteria in addition, generally speaking, reach the object improving sewage treating efficiency.
Embodiment
Embodiment 1
The method of microorganism and waterplant synergistic purification polluted-water, comprises the following steps,
A. choose the microorganism slow release tube that caliber is 16mm, length is 10cm, microorganism slow release tube two seals, and pipe shaft spiles, and fills 0.05g complex microorganism preparations in microorganism slow release tube pipe;
On the root of B. submerged plant, bundle a microorganism slow release tube, be the submerged plant that in the bed mud of 0.5 meter, plantation binding has microorganism slow release tube in the depth of water, and microorganism slow release tube is in bed mud or in water;
C. along with the growth of submerged plant, the microorganism in microorganism slow release tube pipe progressively discharges, and is attached on submerged plant, forms microbial film.
Embodiment 2
The method of microorganism and waterplant synergistic purification polluted-water, comprises the following steps,
A. choose the microorganism slow release tube that caliber is 16mm, length is 15cm, microorganism slow release tube two seals, and pipe shaft spiles, and fills 0.075g complex microorganism preparations in microorganism slow release tube pipe;
B. a binding microorganism slow release tube on the root of every strain submerged plant or stem, be in the bed mud of 0.8 meter in the depth of water, plantation binding has the submerged plant of microorganism slow release tube.
C. along with the growth of submerged plant, the microorganism in microorganism slow release tube pipe progressively discharges, and is attached on submerged plant, forms microbial film.
Wherein, microorganism slow release tube is pvc pipe.
Embodiment 3
The method of microorganism and waterplant synergistic purification polluted-water, comprises the following steps,
A. choose the microorganism slow release tube that caliber is 16mm, length is 12cm, microorganism slow release tube two seals, and pipe shaft spiles, and fills 0.1g complex microorganism preparations in microorganism slow release tube pipe;
B. a binding microorganism slow release tube on the root of every strain submerged plant or stem, be in the bed mud of 2 meters in the depth of water, plantation binding has the submerged plant of microorganism slow release tube.
C. along with the growth of submerged plant, the microorganism in microorganism slow release tube pipe progressively discharges, and is attached on submerged plant, forms microbial film.
Wherein, microorganism slow release tube is pvc pipe.
Complex microorganism preparations, comprises microorganism and prozyme two class component:
(1) microorganism is the Bacillus licheniformis BFT-4 (BacillusLicheniformis BFT-4) of CCTCC NO:M2011423 by preserving number, preserving number is the Pseudomonas stutzeri BRH-(6 Pseudomonasstutzeri BRH-6) of CCTCC NO:M2011425, with pseudomonas putida BRZ-1 (Pseudomonasputida BRZ-1) composition that preserving number is CCTCC NO:M2011427, wherein, the mass percent of described each component microorganism in microbial total quality is: Bacillus licheniformis BFT-430-60%, Pseudomonas stutzeri BRH-625-35%, pseudomonas putida BRZ-115-35%,
(2) prozyme comprises proteolytic enzyme, amylase and cellulase, and the mass percent of each component of prozyme is: proteolytic enzyme 40%-60%, amylase 15-25%, cellulase 25%-35%, and wherein, enzyme is lived and is all not less than 10,000 U/g.
Embodiment 4
The method of microorganism and waterplant synergistic purification polluted-water, comprises the following steps,
A. choose the microorganism slow release tube that caliber is 16mm, length is 20cm, microorganism slow release tube two seals, and pipe shaft spiles, and fills 0.1g complex microorganism preparations in microorganism slow release tube pipe;
B. 10 strain plant planktons are divided into one clump, tie up microorganism slow release tube in the middle of one clump of plant plankton, then planted in plant plankton framework.
C. along with the growth of plant plankton, the microorganism in microorganism slow release tube pipe progressively discharges, and is attached on submerged plant, forms microbial film.
Embodiment 5
The method of microorganism and waterplant synergistic purification polluted-water, comprises the following steps,
A. choose the microorganism slow release tube that caliber is 16mm, length is 15cm, microorganism slow release tube two seals, and pipe shaft spiles, and fills 0.05g complex microorganism preparations in microorganism slow release tube pipe;
B. 10 strain plant planktons are divided into one clump, tie up microorganism slow release tube in the middle of one clump of plant plankton, and be bundled on the root of plant plankton, then planted in plant plankton framework.
C. along with the growth of plant plankton, the microorganism in microorganism slow release tube pipe progressively discharges, and is attached on submerged plant, forms microbial film.
Wherein, microorganism slow release tube is pvc pipe.
Embodiment 6
The method of microorganism and waterplant synergistic purification polluted-water, comprises the following steps,
A. choose the microorganism slow release tube that caliber is 16mm, length is 10-20cm, microorganism slow release tube two seals, and pipe shaft spiles, and fills 0.08g complex microorganism preparations in microorganism slow release tube pipe; Submerged plant chooses the microorganism slow release tube that length is 20cm.
B. 10 strain plant planktons are divided into one clump, tie up microorganism slow release tube in the middle of one clump of plant plankton, and be bundled on the stem of plant plankton, then planted in plant plankton framework.
C. along with the growth of plant plankton, the microorganism in microorganism slow release tube pipe progressively discharges, and is attached on submerged plant, forms microbial film.
Complex microorganism preparations, comprises microorganism and prozyme two class component:
(1) microorganism is the Bacillus licheniformis BFT-4 (BacillusLicheniformis BFT-4) of CCTCC NO:M2011423 by preserving number, preserving number is the Pseudomonas stutzeri BRH-(6 Pseudomonasstutzeri BRH-6) of CCTCC NO:M2011425, with pseudomonas putida BRZ-1 (Pseudomonasputida BRZ-1) composition that preserving number is CCTCC NO:M2011427, wherein, the mass percent of described each component microorganism in microbial total quality is: Bacillus licheniformis BFT-430-60%, Pseudomonas stutzeri BRH-625-35%, pseudomonas putida BRZ-115-35%,
(2) prozyme comprises proteolytic enzyme, amylase and cellulase, and the mass percent of each component of prozyme is: proteolytic enzyme 40%-60%, amylase 15-25%, cellulase 25%-35%, and wherein, enzyme is lived and is all not less than 10,000 U/g.
Embodiment 7
The method of microorganism and waterplant synergistic purification polluted-water, comprises the following steps,
A. choose the microorganism slow release tube that caliber is 16mm, length is 12cm, microorganism slow release tube two seals, and pipe shaft spiles, and fills 0.06g complex microorganism preparations in microorganism slow release tube pipe;
A binding microorganism slow release tube on the stem of B. every strain submerged plant, be in the bed mud of 2 meters in the depth of water, plantation binding has the submerged plant of microorganism slow release tube;
C. 10 strain plant planktons are divided into one clump, tie up microorganism slow release tube in the middle of one clump of plant plankton, then planted in plant plankton framework;
D. the microorganism in microorganism slow release tube pipe progressively discharges, and is attached on submerged plant, forms microbial film.
Wherein, microorganism slow release tube is pvc pipe.
In a word, the foregoing is only preferred embodiment of the present invention, all equalizations done according to the present patent application the scope of the claims change and modify, and all should belong to the covering scope of patent of the present invention.
Claims (7)
1. the method for microorganism and waterplant synergistic purification polluted-water, is characterized in that: comprise the following steps,
A. choose the microorganism slow release tube that caliber is 16mm, length is 10-20cm, microorganism slow release tube two seals, and pipe shaft spiles, and fills 0.05-0.1g complex microorganism preparations in microorganism slow release tube pipe;
B. on the root of waterplant or stem, bundle a microorganism slow release tube, be that in the bed mud of 0.5-2 rice, plantation binding has the waterplant of microorganism slow release tube and/or plantation in plant plankton framework in the depth of water, and microorganism slow release tube is in bed mud or in water;
C. along with the growth of waterplant, the microorganism in microorganism slow release tube pipe progressively discharges, and is attached on submerged plant, forms microbial film.
2. the method for microorganism according to claim 1 and waterplant synergistic purification polluted-water, is characterized in that: microorganism slow release tube is pvc pipe.
3. the method for microorganism according to claim 1 and 2 and waterplant synergistic purification polluted-water, is characterized in that: waterplant is submerged plant or plant plankton.
4. the method for microorganism according to claim 3 and waterplant synergistic purification polluted-water, it is characterized in that: in step B, the root of every strain submerged plant or stem bundle a microorganism slow release tube, be in the bed mud of 0.5-2 rice in the depth of water, plantation binding has the submerged plant of microorganism slow release tube.
5. the method for microorganism according to claim 4 and waterplant synergistic purification polluted-water, is characterized in that: plant plankton chooses the microorganism slow release tube that length is 10cm.
6. the method for microorganism according to claim 3 and waterplant synergistic purification polluted-water, it is characterized in that: in step B, 10 strain plant planktons are divided into one clump, tie up microorganism slow release tube in the middle of one clump of plant plankton, then planted in plant plankton framework.
7. the method for microorganism according to claim 6 and waterplant synergistic purification polluted-water, is characterized in that: submerged plant chooses the microorganism slow release tube that length is 20cm.
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Cited By (6)
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| CN105060498A (en) * | 2015-07-20 | 2015-11-18 | 成都市昊麟科技发展有限责任公司 | Biological strain embedding device for pre-processing biological reaction tank and processing technology |
| CN105417688A (en) * | 2015-11-03 | 2016-03-23 | 重庆乐善环保科技有限公司 | Spherical filler and special-shaped carbon filler and their applications |
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| CN108439608A (en) * | 2018-06-14 | 2018-08-24 | 安徽省大地园林工程有限公司 | A kind of wetland park water plant water environment purification method |
| CN109678246A (en) * | 2018-10-31 | 2019-04-26 | 深圳地大水务工程有限公司 | A method of suitable for lake and reservoir, unhurried current type river regulation wawter bloom |
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