CN104521535A - Morphological identification method for Miscanthus sinensis and M. lutarioriparius hybrid - Google Patents

Abstract

The invention discloses a morphological identification method for Miscanthus sinensis and M. lutarioriparius hybrid. The method comprises the steps of sowing, seedling raising, transplanting, field planting, and morphological observation, wherein the morphological observation mainly includes tillering stage observation, elongation stage observation and heading stage observation. The method is simple, convenient to use and high in accuracy, and omits various detection procedures of molecule levels and cell levels.

Description

A kind of awns (Miscanthus sinensis, ♂) and southern reed (M. lutarioriparius, ♀) crossbreed Morphological Identification method

Technical field

The invention belongs to agriculture hybridization technique application, be specifically related to a kind of be applicable to awns platymiscium two dispersed species---awns (Miscanthus sinensis) with southern reed (M.lutarioriparius) filial generation, the authenticate technology of true crossbreed.

Background technology

Awns belongs to (Miscanthus) and belongs to perennial tall and big grass family, and biomass is large, widely distributed.China is the center of awns platymiscium distribution, and when global fossil energy scarcity, awns genus attracts wide attention and research as second generation power source crop.At the propagation and employment that America and Europe belongs to along with awns, find natural hybrid species strange ridge adaptability narrow, cannot be solid, this brings obstruction to large-scale production.

Nan Di belongs to Chinese unique wheat, and awns also has a very wide distribution in China, has a large amount of Wild ornamental resources.The biological property that wild southern reed germ plasm resource has is that subterranean stem is scattered, and internode has axillalry bud, and glumelle is without awns point; Awns subterranean stem is grown thickly, and internode is without axillalry bud, and glumelle has awns point.Be better than Nan Di from adaptability aspect awns, awns can grow in arid desert, and southern reed is then mainly distributed in beach area, river.But southern reed biomass and living beings are better than awns.In conjunction with both superior cultivation be suitable for the important research content becoming breeding man in the new varieties of the barren popularization of arid.But detecting its authenticity after obtaining crossbreed is matter of utmost importance.Developing along with molecular approach has gone out the method for many qualification crossbreed, and as utilized molecular marking technique, the methods such as Physiology and biochemistry, these technical conditions require harsh, and complicated operation, needs professional, necessarily increases production cost.Simply be easy to grasp by typical Morphological Identification crossbreed method, cost is low, is applicable to large-scale promotion.

Summary of the invention

The object of this invention is to provide a kind of Morphological Identification method of awns and southern reed interspecific hybrid offspring, the method is applicable to awns platymiscium genetic breeding research.The method is simple and easy to grasp, the accuracy of the selection result is high, solve the problem needing many limiting factor (s)s such as a large amount of background information, various indispensable technical supports in conventional hybridization kind authentication method well, the true crossbreed of awns platymiscium Interspecific Hybrids can be identified fast, accurately and efficiently.

The object of the invention is logical following manner realize:

A kind of awns (Miscanthus sinensis, ♂) and southern reed (M.lutarioriparius, ♀) crossbreed Morphological Identification method, comprise the following steps:

1) seeding and seedling raising: with awns (Miscanthus sinensis, ♂) and the planting seed nursery of gathering in the crops southern reed (M.lutarioriparius, ♀) hybridization;

2) transplant planting: the seed for seedling carries out Plant colonization;

3) observation in tillering stage: after plant to be planted enters tillering stage, if there is individual plant plant type now to grow thickly in progeny population, then tentatively can regard as crossbreed;

4) shooting stage observation: to step 3) after the preliminary crossbreed individual plant plant assert enters the shooting stage, push leaf sheath aside and observe joint portion and have axillalry bud, then can regard as crossbreed further;

5) observation at heading stage, after entering heading stage, the awns on observation inflorescence small ear glumelle and basal disc hair, if there is basal disc hair short 2-3mm more coetonium than small ear in small ear, and glumelle has the situation of the long awns point of 4-6mm, then finally regards as true crossbreed.

The present invention judges the hybridization true and false by crossbreed morphological feature, and without the need to Laboratory Instruments medicine and experiment correlation technique, simple to operate, be easy to grasp, can carry out anywhere, without site requirements, cost is low, can be promoted on a large scale in production practices.

Accompanying drawing explanation

Fig. 1 is that southern reed is scattered;

Fig. 2 is that awns grows thickly;

Fig. 3 is that crossbreed subterranean stem is tillered; Visible individual plant plant type is now grown thickly;

Fig. 4 is the stem section joint portion after pushing leaf sheath aside; Left: awns stem section, middle 3 are hybridization stem section successively, right: southern reed stem section);

Fig. 5 is sub-lemma in Grain in Ear;

Fig. 6 is southern reed seed lemma;

Fig. 7 is crossbreed seed lemma;

Fig. 8 is crossbreed (left side) and non-crossbreed (right side) comparison diagram.

Embodiment

Be intended to further illustrate the present invention below in conjunction with embodiment, and unrestricted the present invention.

Embodiment 1:

With Agricultural University Of Hunan awns belong to preserve in germplasm resource garden awns male parent (plantation number: B0605, locality: Guangxi Gold show.) (to plant number: A0107, locality: Changsha with southern reed female parent.) screening of crossbreed is example.

1, seeding and seedling raising: mid or late March, seeding and seedling raising in greenhouse, keeps environmental temperature about 25 DEG C, and air humidity keeps about 80%.Through the seedling management of 40-50 days, regularly weed a garden therebetween,

2, transplant planting: treat that height of seedling is to about 10-15cm, select carry out transplant planting the fine morning, seeding row spacing is from 1 × 2m.

3, observation in tillering stage: tillering stage judges plant type, is tentatively defined as crossbreed.

The seed that A0107 inflorescence is gathered, after plant to be planted enters tillering stage after seedling, if there is individual plant plant type to be scattered (namely having root-like stock to occur) in progeny population, then tentatively can regard as crossbreed.

4, the shooting stage observes further to doubtful crossbreed, again determines according to cane morphological feature,

After shooting stage is entered for the individual plant plant tentatively regarding as doubtful crossbreed, if cane by leaf sheath parcel, push leaf sheath aside and observe that joint portion is smooth axillalry bud, then can regard as crossbreed further.

5, judge small ear heading stage, the awns on observation inflorescence small ear glumelle and basal disc hair, if occur in small ear, basal disc hair is significantly shorter than the coetonium 2.11mm of small ear, and glumelle has longer awns point 4.22mm, finally determines true crossbreed.

6, by Yield compari@, crossbreed per mu yield, male parent (B0605) per mu yield, maternal (A0107) per mu yield,

Stem stalk main chemical compositions compares, crossbreed cellulose, hemicellulose, and lignin and total ash are respectively 48.93%, 31.05%, 1.89% and 2.82%.The above index of male parent is 40.30%, 32.55%, 13.93% and 6.56%.Maternal above index 49.92%, 30.40%, 15.28% and 2.91%.

7, Matrix attachment region rDNA ITS sequence sequencing analysis result shows crossbreed, and female parent is southern reed (A0107), and male parent is awns (B0605).

Claims (1)

1. awns (a Miscanthus sinensis, ) and southern reed (M.lutarioriparius, ♀) crossbreed Morphological Identification method, it is characterized in that, comprise the following steps:

1) seeding and seedling raising: with awns (Miscanthus sinensis, ) with the planting seed nursery of gathering in the crops southern reed (M.lutarioriparius, ♀) hybridization;

2) transplant planting: the seed for seedling carries out Plant colonization;

3) observation in tillering stage: after plant to be planted enters tillering stage, if there is individual plant plant type now to grow thickly in progeny population, then tentatively can regard as crossbreed;

4) shooting stage observation: to step 3) after the preliminary crossbreed individual plant plant assert enters the shooting stage, push leaf sheath aside and observe joint portion and have axillalry bud, then can regard as crossbreed further;

5) observation at heading stage, after entering heading stage, the awns on observation inflorescence small ear glumelle and basal disc hair, if there is basal disc hair short 2-3mm more coetonium than small ear in small ear, and glumelle has the situation of the long awns point of 4-6mm, then finally regards as true crossbreed.