CN104491092A - Composition for refreshing brain and regaining consciousness and preparation method thereof - Google Patents
Composition for refreshing brain and regaining consciousness and preparation method thereof Download PDFInfo
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Abstract
The invention relates to a composition for refreshing brain and regaining consciousness. The composition is prepared from the following components in parts by weight: 50-90 parts of menthol, 20-60 parts of menthyl lactate, 5-30 parts of borneol, 0.4-1.2 parts of heat a sensitizer and the balance of ethanol, and further comprises 20-40 parts of camphor, 5-30 parts of ginseng leaf oil and 10-40 parts of rosemary leaf oil. The composition is capable of playing roles in refreshing and cooling in early, intermediate and late phases of application, is quick in response and durable in action, can be used for all-round comprehensively refreshing brain by means of combination of cooling and fragrance, has more remarkable brain-refreshing and consciousness-regaining effects than those of similar products, can be directly applied to skin, and is safe, comfortable and convenient to use.
Description
Technical field
The invention belongs to medicines and health protection field, be specifically related to a kind of refreshment compositions and preparation method.
Background technology
In recent years, lassitude in sub-health population, sleepiness is unable, feeling of fullness in the head is forgetful etc., and various fatigue symptom grows with each passing day.The normal physiological phenomenon of one that fatigue must occur when being the mental or physical arrival certain phase of people, due to the consumption of each organ energy matter and the accumulative of lactic acid metabolic wastes, the original ability to work of body can temporarily decline.In order to the fatigue of tackling, the modern times, most usual way was that cool refreshing and fragrance are refreshed oneself, just these two kinds of methods can the only use when needs, can not produce apparent side effect, all the fashion because of its safety and motility.
Because mental fatigue often produces the symptoms such as impetuous, dizzy, cold-type yellow (clear) product can clearing away heat and expelling pathogen in the exterior, local cooling, have protective effect to brain cell, brains of cooling down, clarification the state of mind.Applying maximum in this series products is take essential balm as topical drug and the cosmetics of representative, cooling ingredient wherein with Mentholum and Borneolum Syntheticum for Typical Representative (the visible the Sanitation Ministry medicine standard Traditional Chinese medicine historical preparation of its formula, usage and effect the 9th WS3-B-1708-94), after outer wiping skin, can local blood circulation be accelerated and dispel the heat on the one hand, the tip that can excite nerve on the other hand produces cool feeling, and can namely wipe i.e. use, do not need the exquisite course for the treatment of and life cycle, be thus popular.
But the deficiency of essential balm also clearly: first, cooling effect instantaneous burst, then sharply declining, it is lasting to be difficult to; There is intense stimulus sense to skin, opening eyes for being even difficult to after women's head-ornaments; Owing to adopting petroleum-type product liquid paraffin as solvent, by rear residual neither volatile also not easy cleaning.Given this, this series products can not meet the needs that modern pursues life taste.In recent years, by method gained popularity that fragrance is refreshed oneself.
When human central nervous is suppressed, state that every physiological function can be in " dozing off ", and during central excitation, by vegetative nerve adjustment, makes heart beating speed, exaggerated respiration, glandular secretion increases, therefore full of vitality.Well-known by the effect of the joyful spirit of spice, stabilizing the emotions, it is calm, clear-headed, excitation is also proved according to mensuration electroencephalogram etc. gradually.Aromatotherapy (champignon) is also widely used in clinical all over the world, the forms such as modern aromatotherapy many employings perfume, Folium Nicotianae preparatum, volatile ingredient wherein produces olfactory sensation via Nasal Mucosa Absorption, exchanges and enters body circulation, jointly inspire nervus centralis calm or excited through pulmonary gases.But to neural activation, still only some is reported out in aromatotherapy, and adopts a form tool instantaneous results of Folium Nicotianae preparatum, is not easy to continue to smoke; And perfume is only heard by nose usually, the suction of effective ingredient is very limited.
In view of cool refreshing product only plays the refrigerant stimulation in local, and champignon therapy only by nose hear smell inhale limited to central excitation, if get the length of the two, develop a kind of external preparation, embrocate the skin in main acupuncture point, as Renzhong acupoint, temple etc., both can local refrigerant stimulation, can pass through again skin percutaneous absorbtion, also can play champignon effect, this product certainly will have wide market prospect, and this kind of formula is also the striving direction of medical scientific research worker.
The volatile oil adopted in modern aromatotherapy just can blend massage by short, enters body circulation, so more meet the champignon instructions for use of modern through skin percutaneous absorbtion.Such as, Chinese invention patent ZL 201010104195.6, discloses compound essential Oil and the wet paper towel of a kind of resisting fatigue and refreshment, compound essential Oil optimization formula is: menthol 30 parts, fragrant cananga caul-fat 1 part, Oleum lavandula angustifolia 22 parts, Herba Rosmarini Officinalis leaf oil 7 parts, oleum Citri sinensis 28 parts, cineole 22 parts, petit grain lemon oil 25 parts, Oleum menthae 0.12 part, Oleum Caryophylli 35 parts, Oleum Menthae Rotundifoliae 40 parts.Dry non-woven fabrics is immersed in compound essential Oil, obtains the wet paper towel of refreshment resisting fatigue.Usage is external, and erasable, in Renzhong acupoint, temple, Yintang acupoint and Fengchi acupoint, also directly can smell suction.Carefully analyze and can find also there are 3 deficiencies:
1) this product only has menthol to be cooling ingredient, and its effect is of short duration, and it is very limited that the form of wet tissue also determines refrigerant effect;
2) quintessence oil is directly used in skin and has and burn or excitement;
3) in wet tissue containing the bad assurance of quintessence oil amount, as more dry in wet tissue then only by nasil effective ingredient very little, as too wet, quintessence oil amount is used for skin may have stimulation.
Summary of the invention
The object of this invention is to provide a kind of refreshment composition and method of making the same, can quick acting and the cooling effect persistent period long, refreshment more remarkable effect.
Compared to the menthol that ZL 201010104195.6 only adopts cooling effect of short duration, the present invention considers refrigerant rapid-action, for a long time lasting especially, filter out menthol, menthanol lactic acid ester, Borneolum Syntheticum, heat sensitizer and ethanol through lot of experiments, realize the quick acting of cooling effect, persistent.Unique, this combination can make each cooling ingredient play a role successively, is the refrigerant sense of menthol chafe prompt explosion at the beginning of use, and mid-term is that ethanol cooling of progressively volatilizing is refrigerant, and it is refrigerant that the later stage is that menthanol lactic acid ester continues.With VBE with the use of, according to the principle of refrigerant illusion, creeping chill time and intensity can be strengthened.Through lot of experiments research, menthol: menthanol lactic acid ester: Borneolum Syntheticum: heat sensitizer weight part ratio is (50 ~ 90 parts): (20 ~ 60 parts): (5 ~ 30 parts): (0.4 ~ 1.2 part) can reach good effect.Heat sensitizer wherein can be VBE or butyl Rhizoma et radix valerianae ester, preferably VBE.
As improvement, heat sensitizer adopts VBE, menthol: menthanol lactic acid ester: Borneolum Syntheticum: VBE weight part ratio is (65 ~ 90): (20 ~ 45): (5 ~ 20): (0. 4 ~ 1.2), under the marking mode of the specific embodiment of the invention, higher than 70 points.
As further improvement, menthol: menthanol lactic acid ester: Borneolum Syntheticum: VBE weight ratio is (65 ~ 75): (28 ~ 42): (5 ~ 18): (0.4 ~ 0.82), under the marking mode of the specific embodiment of the invention, higher than 80 points.Further, weight ratio is (68 ~ 72): (39 ~ 41): (5 ~ 16): (0.78 ~ 0.82), higher than 95 points.In one more preferred embodiment, weight ratio is 70:40:5:0.8, and in most preferred embodiment, weight ratio is 70:40:15:0.8.
With above-mentioned weight for benchmark, the amount of ethanol is preferably 200 ~ 400 parts.Too fast for preventing ethanol from volatilizing, keep lastingly refrigerant, the present invention also adds glycerol (or propylene glycol), is preferably 20 ~ 40 parts.
Above-mentionedly mainly to be refreshed oneself by refrigerant component.In order to reach effect of better refreshing oneself, the invention above-mentioned refrigerant component is combined with champignon, by adding Radix Ginseng leaf oil, oil of rosemary, Camphora, form refreshment with Borneolum Syntheticum, the core of excitor nerve combines, can mutually promote with above-mentioned refrigerant component, effect is refreshed oneself in collaborative performance.By scientific matching, can Energy Metabolism of Brain Tissue be improved, promote that energy matter utilizes, certain heart tonifying and excited Repiration can be played, also can play dual regulation, be conducive to the balance of nervus centralis function, protection cerebral tissue.With the proportioning of above-mentioned refrigerant component for benchmark, optimum ratio is: Camphora 20 ~ 40 parts, Radix Ginseng leaf oil 5 ~ 30 parts, Herba Rosmarini Officinalis leaf oil 10 ~ 40 parts, can reach collaborative with each refrigerant component and play a role.Be more preferably: Camphora 25 ~ 35 parts, Radix Ginseng leaf oil 10 ~ 15 parts, Herba Rosmarini Officinalis leaf oil 10 ~ 20 parts.
In order to the effect making the present invention have pleasure, the present invention adopts oleum Citri sinensis and/or Flos micheliae Albae oil to coordinate, and abnormal smells from the patient is pure and fresh, sweet, graceful, contributes to eliminating anxiety, pleasure, contributes to promoting attention.With above-mentioned weight for benchmark, optimum ratio is oleum Citri sinensis 5 ~ 30 parts and/or Flos micheliae Albae oil 5 ~ 15 parts.Also can use Oleum lavandula angustifolia, thyme oil substitute.
Because each quintessence oil is the volatile micromolecule composition of tool, skin can absorb sub-fraction, and for fully absorbing onset, the technical program adopts the combination of menthol, Borneolum Syntheticum, ethanol as penetrating agent.Menthol and Borneolum Syntheticum are respond well dermal osmosis accelerators, according to the difference of different material character, add this combination penetrating agent of 3 ~ 10%, and the amount that plant volatile oil can be made to enter body circulation through skin increases several times.Ethanol is then good solvent, increases medicine at cuticular dissolubility, plays auxiliary transdermal enhancing effect.
Pure plants essential oil can not be directly used in and embrocate skin, and this is the common recognition of insider.For this point, the technical program is furnished with oil and solvent carrier based on castor oil hydrogenated, glycerol (or propylene glycol), second alcohol and water.With above-mentioned weight for benchmark, preferably add castor oil hydrogenated 120 ~ 180 parts, 200 ~ 400 parts, water.
In the above-mentioned formula of the present invention, the function of main component is:
Menthol (Mentholum): merit is apt to wind-dispelling heat-dissipating, refresh oneself, has the pungent fragrance taste of strong Herba Menthae speciality.Can make skin capillary vascular expansion, promote sweat gland secretion, have the effect of sweating and heat resolving, the tip that can excite nerve again produces cool feeling, feels well pleasant for skin ice.Also the skin penetration enhancer in medicine is often used as;
Menthanol lactic acid ester: effect is similar to menthol, the main distinction is that the refrigerant effect explosive force of menthol is strong, and the persistent period is short, and menthanol lactic acid ester abnormal smells from the patient is very light, and refrigerant onset of action is slightly slow, and the persistent period is longer;
Borneolum Syntheticum: the kind refreshment of having one's ideas straightened out of merit, clearing away heat to alleviate pain.Have excited to nervus centralis and suppress dual regulation effect, its main component Borneolum Syntheticum can improve ischemic tissue of brain energy metabolism, alleviates brain injury.It is strong that the pungent perfume (or spice) of this product walks to alter power, is good shortly to ooze penetration enhancer, even has good transdermal enhancing effect to agent permeates therethrough blood brain barrier.Coordinate with Mentholum and can suitably increase refrigerant sense;
Camphora: have obvious excitation to higher nervous center, in vivo through metabolic conversion, has obvious heart tonifying, boosting and excited Repiration, but answers control dose unsuitable excessive, otherwise easily occurs superexcitation;
Radix Ginseng leaf oil: it is top grade that Radix Ginseng is always regarded as that tonify deficiency reposes, Radix Ginseng leaf oil is more precious, ginsenoside wherein can promote that brain is to the utilization of energy matter, low dose of have excitation to nervus centralis, heavy dose is then mainly inhibitory action, and this dual regulation is conducive to the balance of nervus centralis function;
Herba Rosmarini Officinalis: refreshment, convergence skin.Someone carries out plus maze and step down test by rabbit, indicates effect of its anxiety and enhancing ability of learning and memory, contributes to promoting attention;
Oleum Citri sinensis: abnormal smells from the patient is fresh, sweet, pleasant, energy fatigue alleviating, antidepressant;
Flos micheliae Albae oil: abnormal smells from the patient is pure and fresh, graceful, contributes to eliminating anxiety, rousing oneself body and mind;
Castor oil hydrogenated: by Oleum Ricini hydrogenation is a kind of good solubilizing agent, when content is 15 ~ 20%, multi-component compound essential Oil can be made the liquid of clear homogeneous, also can be used as the base oil of dilution quintessence oil, has certain protective effect to skin;
VBE: be long-acting heat sensitizer, can blood circulation promoting; Other heat sensitizers available substitute, as butyl Rhizoma et radix valerianae ester;
Ethanol: the good solvent being preparation quintessence oil, perfume etc., has sterilization antisepsis, again due to its effumability, can take away heat, also has the refrigerant effect of cooling;
Glycerol: matter thickness, water absorbing capacity is strong; Be used for protecting skin, prevent ethanol from volatilizing too fast, keep product stability; Using propylene glycol substitutes.
To sum up, for the deficiency that commercially available cool refreshing product quick acting and cooling effect persistent period can not be taken into account, the multiple cooling ingredient of employing of the invention, initial stage after a procedure, mid-term, later stage play refrigerant cooling-down effect successively, quick acting, persistent, thus play the cool refreshing effect continued, meet the clarification state of mind better, improve the needs of attention.Special population can be met namely imitate as namely fatigue driving personage wipes, the object of a period of time can be maintained again.
The present invention is combined with champignon by refrigerant again, and multi-direction synthetic is refreshed oneself, and refreshment effect is more remarkable than like product.Both the conventional cooling ingredient such as Herba Menthae, Borneolum Syntheticum had been adopted, Folium Ginseng, Herba Rosmarini Officinalis etc. is more adopted to have the Chinese medicine of unique regulating action to nervus centralis, main derived essential oil, by nasal mucosa or pulmonary absorption, also can pass through body surface and absorbs through skin, acupuncture point multipath, play whole body regulating action.Both refrigerant cooling protected brain with refreshment, and excite nerve again activation physical function, played anti-fatigue effect of refreshing oneself significantly.
In addition, the present invention does not need to allocate dilution again, can be directly used in skin, and without pessimal stimulation, elegant fragrance is pure and fresh, and skinfeel is good, harmless, non-greasy, is easy to water cleaning, safe, comfortable, easy to use.
The present invention have passed through a large amount of combination preferably, has taken into full account that cooling effect is best, and Transdermal absorption is optimum, and pleasing affect and the excitor nerve of spice are laid equal stress on.With the ZL 201010104195.6(introduced in background technology hereinafter referred to as contrasting) scheme compared with:
(1) the present invention adopts multiple cooling ingredient composite, can accomplish onset in 1 minute, continues 1 hour; And only have menthol can play instantaneous refrigerant effect by chafe in the quintessence oil wet paper towel of contrast, without the effect of ethanol volatilization radiating and cooling, do not consider lasting effect yet;
(2) the quintessence oil wet paper towel contrasted does not take into full account that skin is thoroughly short, mainly hears by nose and breathes a kind of approach onset; And the present invention refrigerantly combines with champignon, by multipaths such as the stimulation of skin cool feeling, Transdermal absorption, breathings, can utilize functional component fully, play more significant cool refreshing, excited refreshing function;
(3) composition in the quintessence oil wet paper towel contrasted is pure quintessence oil, is not suitable for being directly used in skin; The present invention is furnished with base oil, can be directly used in skin, comfortable, without pessimal stimulation, and non-greasy, be easy to clean with water;
(4) the quintessence oil wet paper towel contrasted is without the Radix Ginseng leaf oil of excited maincenter, and leaf of Herba Rosmarini Officinalis oil content is also less, only containing 7 parts, is again by wet paper towel wiping or smells news, wherein absorbed by the body can the composition of excitor nerve less; It can thus be appreciated that, the present invention to the excitement of maincenter and regulating effect more outstanding.
The preparation method of refreshment compositions of the present invention, comprises the steps:
1) menthol, Herba Menthae lactate, Borneolum Syntheticum, Camphora and ethanol are mixed, preparing alcohol phase, is colourless transparent solution;
2) by Radix Ginseng leaf oil, Herba Rosmarini Officinalis leaf oil, oleum Citri sinensis, Flos micheliae Albae oil, castor oil hydrogenated, VBE mixing, preparing oil phase, is homogeneous transparent oily liquids;
3) G & W is mixed, prepare aqueous phase;
4) under normal temperature condition, oil phase is joined alcohol phase, stir, then aqueous phase is added wherein, stir, be prepared into semi-finished product;
5) above-mentioned semi-finished product are placed in hermetic container, sealing is preserved, and allows each fragrance component fully merge ageing.
Above-mentioned preparation method is in room temperature preparation, does not need heating, can ensure that product is even, stable, non-volatile.Must follow charging sequence, namely first oil phase be joined alcohol phase, then aqueous phase is joined in oleyl alcohol mixed phase, otherwise it is irregular to have mixed dissolving, or have floccule to separate out.
Accompanying drawing explanation
Accompanying drawing 1 is Mentholum transdermal absorption factor rectangular histogram.
Detailed description of the invention
Volatile oil crystallization by medical material vapor distillation, then can obtain by the raw materials used menthol of the present invention, Borneolum Syntheticum, Camphora, also commercially available.Herba Rosmarini Officinalis, the quintessence oil such as Fructus Citri sinensis, Flos micheliae Albae is the pure plants essential oil that vapor distillation obtains, commercially available.The pure plants essential oil that Radix Ginseng leaf oil behaviour Rhizoma panacis majoris obtains through vapor distillation, such as use the steam distillation of about 100 DEG C, condensation must slip out liquid, and stratification obtains, commercially available.All the other raw materials of the present invention are all commercially available.
In further detail the present invention is described by the following examples, is below by weight.
embodiment 1
Formula: menthol 80 parts, menthanol lactic acid ester 50 parts, Borneolum Syntheticum 20 parts, Camphora 40 parts, Radix Ginseng leaf oil 15 parts, Herba Rosmarini Officinalis leaf oil 18 parts, oleum Citri sinensis 12 parts, Flos micheliae Albae oil 12 parts, castor oil hydrogenated 180 parts, glycerol 40 parts, VBE 1 part, ethanol 350 parts, Preservative qs, pure water 320 parts.
Preparation method is:
1) get menthol, Herba Menthae lactate, Borneolum Syntheticum, Camphora are fully mixed, stir and melt, then add ethanol, stirring and dissolving is that colourless transparent solution is for subsequent use, is alcohol phase;
2) by Radix Ginseng leaf oil, Herba Rosmarini Officinalis leaf oil, oleum Citri sinensis, Flos micheliae Albae oil, castor oil hydrogenated, VBE mixing, fully stirring into homogeneous transparent oily liquids, is oil phase;
3) glycerol, antiseptic being added to the water, stirring, is aqueous phase;
4) under normal temperature condition, oil phase is joined alcohol phase, after stirring, then aqueous phase is added wherein, stir, obtain semi-finished product;
5) above-mentioned semi-finished product are placed in hermetic container, sealing preservation 3 days, filters after allowing each fragrance component fully merge ageing, pours into the vial of band ball, adds a cover, and packaging, gets product.
Using method: uncap, utilizes the ball of bottleneck evenly to embrocate in places such as Renzhong acupoint, temple, the spaces between the eyebrows, about 0.5mL at every turn.Once smear and can maintain 1 hour, use again when needing.
embodiment 2
Formula: menthol 70 parts, menthanol lactic acid ester 40 parts, Borneolum Syntheticum 15 parts, Camphora 30 parts, Radix Ginseng leaf oil 12 parts, Herba Rosmarini Officinalis leaf oil 15 parts, oleum Citri sinensis 8 parts, Flos micheliae Albae oil 8 parts, castor oil hydrogenated 150 parts, glycerol 30 parts, VBE 0.8 part, ethanol 300 parts, Preservative qs, pure water 300 parts.
Method of preparation and use is with embodiment 1.
embodiment 3
Formula: menthol 60 parts, menthanol lactic acid ester 25 parts, Borneolum Syntheticum 10 parts, Camphora 25 parts, Radix Ginseng leaf oil 20 parts, Herba Rosmarini Officinalis leaf oil 25 parts, oleum Citri sinensis 20 parts, Flos micheliae Albae oil 5 parts, castor oil hydrogenated 135 parts, glycerol 25 parts, VBE 0.6 part, ethanol 200 parts, Preservative qs, pure water 250 parts.
Method of preparation and use is with embodiment 1.
embodiment 4each cooling ingredient proportioning test (orthogonal design optimization formula)
There is in the technical program cool refreshing drugs with function and mainly contain menthol, menthanol lactic acid ester, Borneolum Syntheticum, VBE, therefore select these 4 compositions to be investigation factor.For optimizing optimum formula, using onset time, the persistent period of refreshing oneself, comfort level etc. as evaluation index, adopt orthogonal design optimization formula.Its factor level is in table 1.
Table 1 factor level table
According to the factor level of table 1 and the combination of table 2, make 100g according to above-mentioned preparation method, obtain 9 evenly clear and bright laboratory samples.
Crowd's experiment on probation is carried out to orthogonal test gained 9 laboratory samples.Each group of observation 30 people, wherein driver 5 people, old people 5 people, middle school student 5 people, workman 5 people, brain worker 5 people, women 5 people.Everyone embrocates laboratory sample 6 with the ball bottle that medicinal liquid is housed at temple and Renzhong acupoint and encloses, timing immediately, observes 60 minutes altogether.
Methods of marking: A is cool refreshing onset time, and in 10sec, onset keeps a perfect score, and often postpones 10sec and subtracts 1 point, the highest 10 points, weighted value 3; B is for refreshing oneself the resisting fatigue persistent period, and persistent period 6min remembers 1 point, and lasting 60min keeps a perfect score, the highest 10 points, weighted value 4; C comfort level, the highest 10 points, weighted value 3; Total score is ABC sum.Computing formula is as follows: total score=A × 3+B × 4+C × 3.
Table 2 L
9(3
4) orthogonal table
By assay optimization, intuitive analysis obtains best of breed A
2b
3c
3d
2.Because best of breed does not have in orthogonal table, best in orthogonal table is experiment 6(A
2b
3c
1d
2, the proportioning of four kinds of compositions is 7:4:0.5:0.08), its total score is the highest, is 96 points, and all comparatively this combination is poor in other combinations, for confirming that best of breed number is A
2b
3c
3d
2or A
2b
3c
1d
2, need confirmatory experiment be done.
By best of breed A
2b
3c
3d
2take menthol 7 parts, menthanol lactic acid ester 4 parts, Borneolum Syntheticum 1.5 parts, VBE 0.08 part, the samely prepare inventive samples, the samely carry out crowd's experiment, mark by above-mentioned methods of marking, three all 10 points, total score is 100 points, than 96 points also high 4 points of experiment numbers 6 in table 1, finally determine that the proportioning of these four kinds of compositions is, menthol: menthanol lactic acid ester: Borneolum Syntheticum: the optimum proportioning=7:4:1.5:0.08 of VBE.Borneolum Syntheticum consumption 1.5 to 0.5 is good.
embodiment 5refreshment compound essential Oil safety testing (test of rat acute toxicity on inhalation)
Given the test agent: embodiment 2 sample.
Experimental animal: SD rat, body weight 190-210 gram, each 10 of male and female.By Hunan, Si Laike Jing Da laboratory animal company limited provides.Laboratory animal production licence number: SCXK(Hunan) 2013-0004.With ad lib and drinking-water in test before experiment, its rearing conditions, feedstuff, water quality are all carried out under equal feeding and management condition.
Test method: 20 animals divide two groups, work, test respectively.1m
3lucite toxicant exposure cabinet, inside puts 10 animals.Tested material is the volatilization of electric heater electrified regulation in cabinet, and quiet formula sucks 2 hours, and sample consumption is 4g, is taken out by animal and moves into observation ward, observe 2 weeks after 2 hours.With the symptom of contamination latter 2 weeks interior animals and death toll during observed and recorded contamination.
Result of the test: animal has no obvious poisoning symptom and death, LD
50value is in table 3.
Table 3 rat acute toxicity on inhalation is tested
| Dosage (mg/m 3） | Death toll/test number (female/male) | LD 50Value (mg/m 3） |
| 4000 | 0/10(is female) | ＞4000 |
| 4000 | 0/10(is male) | ＞4000 |
Record rat acute and suck that LD50 value is female, male is all greater than 4000mg/m
3.
embodiment 6transdermal absorption contrast test
1, the content (chromatographic condition) of Mentholum in gas chromatography determination inventive samples
Chromatographic column is Shimadzu CBP-M25-025 (25 m × 0.22 mm, 0.25 μm), and Polyethylene Glycol-20M is immobile phase, carrier gas is nitrogen, column temperature 130 DEG C, and injection port and detector temperature are 230 DEG C, split ratio 20:1, carrier gas: High Purity Nitrogen, flow: 30mLmin
-1, measure with FID flame ionization ditector.
2, experimental technique and result
Sample 1 is the proportioning of the embodiment of the present invention 2; Sample 2 is essential balm, commercial, traditional Chinese medicines Zhun Zi Z42021197 Wuhan Wujing Medicine Co., Ltd, lot number 20140316; Sample 3 is a kind of compound essential Oil of refreshment resisting fatigue and the wet paper towel containing this quintessence oil, patent of invention ZL201010104195.6.
Adopt and improve Franz diffusion cell.Getting the in vitro DABAI Corium Mus of the SD prepared fixes in Franz diffusion cell, and upward, the effective area of diffusion cell is 4.52cm to keratodermatitis.Draw and injected on the rat skin horny layer of diffusion cell by test agent 1mL, in acceptance pool, add normal saline 20mL, 37 DEG C of waters bath with thermostatic control, constantly stir with magnetic stirring apparatus, make medicine Spontaneous release.Respectively at after coating 0.5,1,1.5,2h respectively gets acceptable solution 2mL, supplements 2mL normal saline immediately after sampling in receiving chamber.The acceptable solution taken out adds ethyl acetate 5mL and extracts 3 times, combining extraction liquid, evaporate to dryness in water-bath, and residue anhydrous alcohol solution, is settled to 1.0mL, with 0.45 μm of filtering with microporous membrane, gets the standby survey of subsequent filtrate.Draw each sample subsequent filtrate respectively and contain Mentholum 1mgmL
-1with Camphora 0.5 mgmL
-1the each 1 μ L of mixing reference substance solution, inject Shimadzu GC-2010 Plus gas chromatograph respectively, measure by above-mentioned chromatographic condition, external standard method calculates content and get final product.
With Determination of menthol in inventive samples for 100%, calculate the transdermal absorption factor of each product different sample time, the results are shown in Figure 1.
Experiment shows, the sample transdermal absorption factor of the technical program is the highest, acts on best; Patented product (a kind of compound essential Oil of refreshment resisting fatigue) takes second place, and accumulative transdermal absorption factor is few 10 percentage points of the present invention comparatively; Essential balm is the poorest, and accumulative transdermal absorption factor is few 11 percentage points of the present invention comparatively.
embodiment 7overall efficacy is evaluated
In order to confirm effect of this product, the sample (essential balm, wet paper towel) of itself and two approximation technique schemes being carried out crowd and has tried out comparative experiments.Each group of observation 45 people, wherein driver 6 people, old people 5 people, middle school student 8 people, workman 9 people, brain worker 10 people, women 7 people.Everyone embrocates laboratory sample 6 with the ball bottle that medicinal liquid is housed at temple and Renzhong acupoint and encloses, timing immediately, observes 60 minutes altogether.
Methods of marking: A is cool refreshing onset time, and in 10sec, onset keeps a perfect score, and often postpones 10sec and subtracts 1 point, the highest 10 points, weighted value 3; B is for refreshing oneself the resisting fatigue persistent period, and persistent period 6min remembers 1 point, and lasting 60min keeps a perfect score, the highest 10 points, weighted value 4; C comfort level, the highest 10 points, weighted value 3; Total score is ABC sum.With total score soprano for the best, computational methods are the same, the results are shown in Table 4.
Table 4 this product and market are similar to like product comparison sheet
| Name of product | Sample 1 | Sample 2 | Sample 3 |
| A onset time | 30 | 26 | 24 |
| The B persistent period | 40 | 35 | 31 |
| C comfort level | 30 | 24 | 27 |
| Total score | 100 | 85 | 82 |
Note: sample 1 is the proportioning of the embodiment of the present invention 2; Sample 2 is essential balm, commercial, traditional Chinese medicines Zhun Zi Z42021197 Wuhan Wujing Medicine Co., Ltd, lot number 20140316; Sample 3 is a kind of compound essential Oil of refreshment resisting fatigue and the wet paper towel containing this quintessence oil, patent of invention ZL201010104195.6.
Experiment shows, this product score is the highest, compared with 2 approximate products, no matter is resisting fatigue onset time of refreshing oneself, or the persistent period, and comfort level is all best.
Claims (10)
1. a refreshment compositions, it is characterized in that said composition comprises following component: menthol, menthanol lactic acid ester, Borneolum Syntheticum, heat sensitizer and ethanol, wherein menthol: menthanol lactic acid ester: Borneolum Syntheticum: heat sensitizer weight part ratio is (50 ~ 90 parts): (20 ~ 60 parts): (5 ~ 30 parts): (0.4 ~ 1.2 part).
2. refreshment compositions according to claim 1, is characterized in that described heat sensitizer is VBE or butyl Rhizoma et radix valerianae ester.
3. refreshment compositions according to claim 1 and 2, it is characterized in that described heat sensitizer is VBE, menthol: menthanol lactic acid ester: Borneolum Syntheticum: VBE weight part ratio is (65 ~ 90): (20 ~ 45): (5 ~ 20): (0. 4 ~ 1.2).
4. refreshment compositions according to claim 3, is characterized in that menthol: menthanol lactic acid ester: Borneolum Syntheticum: VBE weight ratio is (68 ~ 72): (39 ~ 41): (5 ~ 16): (0.78 ~ 0.82).
5. the refreshment compositions according to claim 1,2 or 4, is characterized in that described ethanol is 200 ~ 400 parts; Said composition also comprises glycerol or propylene glycol 20 ~ 40 parts.
6. the refreshment compositions according to claim 1,2 or 4, is characterized in that said composition also comprises Camphora 20 ~ 40 parts, Radix Ginseng leaf oil 5 ~ 30 parts and Herba Rosmarini Officinalis leaf oil 10 ~ 40 parts.
7. refreshment compositions according to claim 6, is characterized in that Camphora is 25 ~ 35 parts, Radix Ginseng leaf oil is 10 ~ 15 parts, Herba Rosmarini Officinalis leaf oil is 10 ~ 20 parts.
8. refreshment compositions according to claim 6, is characterized in that said composition also comprises oleum Citri sinensis 5 ~ 30 parts and/or Flos micheliae Albae oil 5 ~ 15 parts, or Oleum lavandula angustifolia and/or thyme oil 10 ~ 45 parts.
9. refreshment compositions according to claim 8, is characterized in that described Radix Ginseng leaf oil behaviour Rhizoma panacis majoris obtains through vapor distillation; Said composition also comprises castor oil hydrogenated 120 ~ 180 parts, 200 ~ 400 parts, water.
10. a preparation method for refreshment compositions, comprises the steps:
1) by 50 ~ 90 parts of menthols, 20 ~ 60 parts of Herba Menthae lactates, 5 ~ 30 parts of Borneolum Syntheticums, 20 ~ 40 parts of Camphoras and 200 ~ 400 parts of ethanol mixing, alcohol phase is prepared;
2) by 5 ~ 30 parts of Radix Ginseng leaf oils, 10 ~ 40 parts of Herba Rosmarini Officinalis leaf oils, 5 ~ 30 parts of oleum Citri sinensis, 5 ~ 15 parts of Flos micheliae Albae oils, 120 ~ 180 parts of castor oil hydrogenated, 0.4 ~ 1.2 part of VBE mixing, oil phase is prepared;
3) by 20 ~ 40 parts of glycerol and 200 ~ 400 parts of water mixing, aqueous phase is prepared;
4) under normal temperature condition, oil phase is joined alcohol phase, stir, then aqueous phase is added wherein, stir, be prepared into semi-finished product;
5) above-mentioned semi-finished product are placed in hermetic container, sealing is preserved.
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| CN105194107A (en) * | 2015-10-27 | 2015-12-30 | 吴青岱 | Spirit-raising and refreshing drug and preparation method thereof |
| CN105596319A (en) * | 2016-01-31 | 2016-05-25 | 杭州睩客科技有限公司 | Borneol fragrant slice and preparation method thereof |
| CN105998460A (en) * | 2016-05-31 | 2016-10-12 | 江西易累克生物科技有限公司 | Composition with efficacies of eliminating sleepiness as well as refreshing mind and preparation method of composition |
| CN107929101A (en) * | 2017-11-17 | 2018-04-20 | 泰合玺健康科技南京有限公司 | Compact massage essential oil and preparation method thereof |
| CN108078840A (en) * | 2017-12-22 | 2018-05-29 | 泉州市晋江恒安卫生科技有限公司 | One kind is refreshed oneself wet tissue liquid |
| CN110123964A (en) * | 2019-05-28 | 2019-08-16 | 蔡壮 | A kind of moxa liquid and preparation method thereof |
| CN114712443A (en) * | 2021-11-30 | 2022-07-08 | 黄山荷琇生物科技有限公司 | Traditional Chinese medicine fermented composition, refreshing gel and preparation method thereof |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105194107A (en) * | 2015-10-27 | 2015-12-30 | 吴青岱 | Spirit-raising and refreshing drug and preparation method thereof |
| CN105596319A (en) * | 2016-01-31 | 2016-05-25 | 杭州睩客科技有限公司 | Borneol fragrant slice and preparation method thereof |
| CN105998460A (en) * | 2016-05-31 | 2016-10-12 | 江西易累克生物科技有限公司 | Composition with efficacies of eliminating sleepiness as well as refreshing mind and preparation method of composition |
| CN107929101A (en) * | 2017-11-17 | 2018-04-20 | 泰合玺健康科技南京有限公司 | Compact massage essential oil and preparation method thereof |
| CN107929101B (en) * | 2017-11-17 | 2021-03-26 | 泰合玺健康科技南京有限公司 | Firming massage essential oil and preparation method thereof |
| CN108078840A (en) * | 2017-12-22 | 2018-05-29 | 泉州市晋江恒安卫生科技有限公司 | One kind is refreshed oneself wet tissue liquid |
| CN108078840B (en) * | 2017-12-22 | 2021-10-15 | 泉州市晋江恒安卫生科技有限公司 | Wet tissue capable of dispersing and refreshing |
| CN110123964A (en) * | 2019-05-28 | 2019-08-16 | 蔡壮 | A kind of moxa liquid and preparation method thereof |
| CN114712443A (en) * | 2021-11-30 | 2022-07-08 | 黄山荷琇生物科技有限公司 | Traditional Chinese medicine fermented composition, refreshing gel and preparation method thereof |
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Effective date of registration: 20190819 Address after: 410000 Wangcheng Avenue 228 Complex Building, Wangcheng Economic and Technological Development Zone, Changsha City, Hunan Province Patentee after: Hunan Bingxi Biotechnology Co., Ltd. Address before: 410200 No. 228 Wangcheng Avenue, Wangcheng County, Changsha Taiwanese Business Investment Zone, Hunan Province Patentee before: Hunan Tianlong Pharmaceutical Co.,Ltd. |