CN104490943A - Poria cocos injection and preparation method thereof - Google Patents
Poria cocos injection and preparation method thereof Download PDFInfo
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- CN104490943A CN104490943A CN201410703513.9A CN201410703513A CN104490943A CN 104490943 A CN104490943 A CN 104490943A CN 201410703513 A CN201410703513 A CN 201410703513A CN 104490943 A CN104490943 A CN 104490943A
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- poria
- injection
- cosolvent
- antioxidant
- antiseptic
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- 238000002347 injection Methods 0.000 title claims abstract description 38
- 239000007924 injection Substances 0.000 title claims abstract description 38
- 238000002360 preparation method Methods 0.000 title claims abstract description 20
- 235000008599 Poria cocos Nutrition 0.000 title claims abstract description 12
- 241001619444 Wolfiporia cocos Species 0.000 title abstract 5
- 239000003963 antioxidant agent Substances 0.000 claims abstract description 14
- 230000003078 antioxidant effect Effects 0.000 claims abstract description 14
- 239000006184 cosolvent Substances 0.000 claims abstract description 14
- 239000002994 raw material Substances 0.000 claims abstract description 13
- 241001619461 Poria <basidiomycete fungus> Species 0.000 claims description 51
- WVDDGKGOMKODPV-UHFFFAOYSA-N Benzyl alcohol Chemical compound OCC1=CC=CC=C1 WVDDGKGOMKODPV-UHFFFAOYSA-N 0.000 claims description 18
- DNIAPMSPPWPWGF-UHFFFAOYSA-N Propylene glycol Chemical compound CC(O)CO DNIAPMSPPWPWGF-UHFFFAOYSA-N 0.000 claims description 18
- 239000000243 solution Substances 0.000 claims description 16
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 16
- 239000000203 mixture Substances 0.000 claims description 15
- 239000008213 purified water Substances 0.000 claims description 15
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 14
- 230000002421 anti-septic effect Effects 0.000 claims description 13
- 235000006708 antioxidants Nutrition 0.000 claims description 13
- DLFVBJFMPXGRIB-UHFFFAOYSA-N Acetamide Chemical compound CC(N)=O DLFVBJFMPXGRIB-UHFFFAOYSA-N 0.000 claims description 10
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 claims description 9
- CIWBSHSKHKDKBQ-JLAZNSOCSA-N Ascorbic acid Chemical compound OC[C@H](O)[C@H]1OC(=O)C(O)=C1O CIWBSHSKHKDKBQ-JLAZNSOCSA-N 0.000 claims description 8
- GEHJYWRUCIMESM-UHFFFAOYSA-L sodium sulfite Chemical group [Na+].[Na+].[O-]S([O-])=O GEHJYWRUCIMESM-UHFFFAOYSA-L 0.000 claims description 8
- 244000197580 Poria cocos Species 0.000 claims description 7
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 claims description 6
- 235000019445 benzyl alcohol Nutrition 0.000 claims description 6
- 238000001914 filtration Methods 0.000 claims description 6
- ABBQHOQBGMUPJH-UHFFFAOYSA-M Sodium salicylate Chemical compound [Na+].OC1=CC=CC=C1C([O-])=O ABBQHOQBGMUPJH-UHFFFAOYSA-M 0.000 claims description 5
- HRZFUMHJMZEROT-UHFFFAOYSA-L sodium disulfite Chemical compound [Na+].[Na+].[O-]S(=O)S([O-])(=O)=O HRZFUMHJMZEROT-UHFFFAOYSA-L 0.000 claims description 5
- 235000010262 sodium metabisulphite Nutrition 0.000 claims description 5
- 229960004025 sodium salicylate Drugs 0.000 claims description 5
- CHHHXKFHOYLYRE-UHFFFAOYSA-M 2,4-Hexadienoic acid, potassium salt (1:1), (2E,4E)- Chemical compound [K+].CC=CC=CC([O-])=O CHHHXKFHOYLYRE-UHFFFAOYSA-M 0.000 claims description 4
- 235000010323 ascorbic acid Nutrition 0.000 claims description 4
- 229960005070 ascorbic acid Drugs 0.000 claims description 4
- 239000011668 ascorbic acid Substances 0.000 claims description 4
- 229960004756 ethanol Drugs 0.000 claims description 4
- 235000010482 polyoxyethylene sorbitan monooleate Nutrition 0.000 claims description 4
- 229920000053 polysorbate 80 Polymers 0.000 claims description 4
- 235000010241 potassium sorbate Nutrition 0.000 claims description 4
- 239000004302 potassium sorbate Substances 0.000 claims description 4
- 229940069338 potassium sorbate Drugs 0.000 claims description 4
- WXMKPNITSTVMEF-UHFFFAOYSA-M sodium benzoate Chemical compound [Na+].[O-]C(=O)C1=CC=CC=C1 WXMKPNITSTVMEF-UHFFFAOYSA-M 0.000 claims description 4
- 235000010234 sodium benzoate Nutrition 0.000 claims description 4
- 239000004299 sodium benzoate Substances 0.000 claims description 4
- 235000010265 sodium sulphite Nutrition 0.000 claims description 4
- 238000001816 cooling Methods 0.000 claims description 3
- 229960000935 dehydrated alcohol Drugs 0.000 claims description 3
- 230000008030 elimination Effects 0.000 claims description 3
- 238000003379 elimination reaction Methods 0.000 claims description 3
- 239000012467 final product Substances 0.000 claims description 3
- 239000002244 precipitate Substances 0.000 claims description 3
- 238000010298 pulverizing process Methods 0.000 claims description 3
- 230000001954 sterilising effect Effects 0.000 claims description 3
- 239000006228 supernatant Substances 0.000 claims description 3
- 241001465754 Metazoa Species 0.000 abstract description 7
- 238000010255 intramuscular injection Methods 0.000 abstract description 7
- 239000007927 intramuscular injection Substances 0.000 abstract description 7
- 239000003814 drug Substances 0.000 abstract description 3
- 230000001575 pathological effect Effects 0.000 abstract description 2
- 208000024891 symptom Diseases 0.000 abstract description 2
- 229940079593 drug Drugs 0.000 abstract 1
- 239000003755 preservative agent Substances 0.000 abstract 1
- 230000002335 preservative effect Effects 0.000 abstract 1
- 239000000047 product Substances 0.000 description 5
- 230000000694 effects Effects 0.000 description 3
- 238000009472 formulation Methods 0.000 description 3
- 230000036039 immunity Effects 0.000 description 3
- 210000000952 spleen Anatomy 0.000 description 3
- 206010012735 Diarrhoea Diseases 0.000 description 2
- 108060003951 Immunoglobulin Proteins 0.000 description 2
- 206010033557 Palpitations Diseases 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 239000008187 granular material Substances 0.000 description 2
- 102000018358 immunoglobulin Human genes 0.000 description 2
- 210000002966 serum Anatomy 0.000 description 2
- 208000031295 Animal disease Diseases 0.000 description 1
- GLCCKUUEBRLFTH-UHFFFAOYSA-N COCOS Chemical compound COCOS GLCCKUUEBRLFTH-UHFFFAOYSA-N 0.000 description 1
- 241000737241 Cocos Species 0.000 description 1
- DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical compound C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 description 1
- 206010062717 Increased upper airway secretion Diseases 0.000 description 1
- 206010022998 Irritability Diseases 0.000 description 1
- 206010030113 Oedema Diseases 0.000 description 1
- 206010030302 Oliguria Diseases 0.000 description 1
- 208000004880 Polyuria Diseases 0.000 description 1
- 241000576755 Sclerotia Species 0.000 description 1
- 208000013738 Sleep Initiation and Maintenance disease Diseases 0.000 description 1
- LSNNMFCWUKXFEE-UHFFFAOYSA-N Sulfurous acid Chemical compound OS(O)=O LSNNMFCWUKXFEE-UHFFFAOYSA-N 0.000 description 1
- 208000012886 Vertigo Diseases 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 230000003110 anti-inflammatory effect Effects 0.000 description 1
- 230000000259 anti-tumor effect Effects 0.000 description 1
- 230000036528 appetite Effects 0.000 description 1
- 235000019789 appetite Nutrition 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- 238000010241 blood sampling Methods 0.000 description 1
- 230000037396 body weight Effects 0.000 description 1
- 230000001914 calming effect Effects 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 235000009508 confectionery Nutrition 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 230000035619 diuresis Effects 0.000 description 1
- 150000004676 glycans Chemical class 0.000 description 1
- 241000411851 herbal medicine Species 0.000 description 1
- 230000000147 hypnotic effect Effects 0.000 description 1
- 206010022437 insomnia Diseases 0.000 description 1
- 238000007918 intramuscular administration Methods 0.000 description 1
- 210000003734 kidney Anatomy 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 210000004185 liver Anatomy 0.000 description 1
- 210000004072 lung Anatomy 0.000 description 1
- 230000014759 maintenance of location Effects 0.000 description 1
- 238000000034 method Methods 0.000 description 1
- UOJMTSCORVQOHS-UHFFFAOYSA-N pachypodol Natural products COc1cc(ccc1O)C2=C(C)C(=O)c3c(O)cc(C)cc3O2 UOJMTSCORVQOHS-UHFFFAOYSA-N 0.000 description 1
- 244000052769 pathogen Species 0.000 description 1
- 230000001717 pathogenic effect Effects 0.000 description 1
- 208000026435 phlegm Diseases 0.000 description 1
- 229920001282 polysaccharide Polymers 0.000 description 1
- 239000005017 polysaccharide Substances 0.000 description 1
- 230000001737 promoting effect Effects 0.000 description 1
- 238000011084 recovery Methods 0.000 description 1
- 230000001105 regulatory effect Effects 0.000 description 1
- 239000011734 sodium Substances 0.000 description 1
- 229910052708 sodium Inorganic materials 0.000 description 1
- 210000002784 stomach Anatomy 0.000 description 1
- 231100000889 vertigo Toxicity 0.000 description 1
- 230000003612 virological effect Effects 0.000 description 1
Classifications
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/06—Fungi, e.g. yeasts
- A61K36/07—Basidiomycota, e.g. Cryptococcus
- A61K36/076—Poria
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K47/00—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient
- A61K47/06—Organic compounds, e.g. natural or synthetic hydrocarbons, polyolefins, mineral oil, petrolatum or ozokerite
- A61K47/08—Organic compounds, e.g. natural or synthetic hydrocarbons, polyolefins, mineral oil, petrolatum or ozokerite containing oxygen, e.g. ethers, acetals, ketones, quinones, aldehydes, peroxides
- A61K47/10—Alcohols; Phenols; Salts thereof, e.g. glycerol; Polyethylene glycols [PEG]; Poloxamers; PEG/POE alkyl ethers
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K9/00—Medicinal preparations characterised by special physical form
- A61K9/0012—Galenical forms characterised by the site of application
- A61K9/0019—Injectable compositions; Intramuscular, intravenous, arterial, subcutaneous administration; Compositions to be administered through the skin in an invasive manner
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K9/00—Medicinal preparations characterised by special physical form
- A61K9/08—Solutions
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
- A61K2236/331—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using water, e.g. cold water, infusion, tea, steam distillation or decoction
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/39—Complex extraction schemes, e.g. fractionation or repeated extraction steps
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/50—Methods involving additional extraction steps
- A61K2236/53—Liquid-solid separation, e.g. centrifugation, sedimentation or crystallization
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/50—Methods involving additional extraction steps
- A61K2236/55—Liquid-liquid separation; Phase separation
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- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- Epidemiology (AREA)
- Pharmacology & Pharmacy (AREA)
- Natural Medicines & Medicinal Plants (AREA)
- General Health & Medical Sciences (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Medicinal Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Mycology (AREA)
- General Chemical & Material Sciences (AREA)
- Dermatology (AREA)
- Oil, Petroleum & Natural Gas (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Alternative & Traditional Medicine (AREA)
- Biotechnology (AREA)
- Botany (AREA)
- Medical Informatics (AREA)
- Microbiology (AREA)
- Medicinal Preparation (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
Abstract
The invention relates to a poria cocos injection and a preparation method thereof. The poria cocos injection is prepared from the following raw materials in parts by weight: 80-120 parts of poria cocos, 0.5-30 parts of cosolvent, 0.1-0.5 part of antioxidant and 0.4-0.8 part of preservative. The poria cocos injection disclosed by the invention is simple in preparation process and easy to realize. The invention discloses an injection which is administrated by means of intramuscular injection, and capable of rapidly alleviating the pathological symptoms of an animal and improving the immunocompetence of the animal and the bioavailability of medicines in the body.
Description
Technical field
The invention belongs to technical field of medicine, be specifically related to a kind of Poria injection and preparation method thereof.
Background technology
Poria be On Polyporaceae Poria [
p oria COCOS (Schw .) W o1f] dry sclerotia.Its sweet in the mouth, light, flat, return lung, stomach, kidney channel.There is promoting diuresis to eliminate damp pathogen, effect of spleen invigorating mind calming.For edema oliguria, phlegm retention vertigo and palpitation, insufficiency of the spleen lack of appetite, have loose bowels in loose stool, irritability, palpitation with fear insomnia III.In Poria, pachyman (Pach ym ose) is main component, cuts off the effects such as the pachymaran of side chain has antitumor, improves immunity of organisms, protects the liver, hypnosis, defying age, antiinflammatory.The physiologically active of pachyman and health care have caused the great interest of medical circle and region of chemistry, therefore the health product of pachyman are on the field seen everywhere.The Indian bread polysaccharide formulation occurred in the market mostly is oral formulations, has no its ejection preparation.
Summary of the invention
The invention provides a kind of Poria injection, add the route of administration of pachyman, improve the bioavailability of pachyman, can be used for treatment animal viral disease, and improve the immunocompetence of animal.
In order to achieve the above object, the present invention is by the following technical solutions:
The invention provides a kind of Poria injection, be made up of the raw material of following weight portion: Poria 80 ~ 120, cosolvent 0.5 ~ 30, antioxidant 0.1 ~ 0.5 and antiseptic 0.4 ~ 0.8.
Described Poria injection, is made up of the raw material of following weight portion: Poria 90 ~ 110, cosolvent 15 ~ 25, antioxidant 0.2 ~ 0.4 and antiseptic 0.5 ~ 0.6.
Described Poria injection, is made up of the raw material of following weight portion: Poria 100, cosolvent 20, antioxidant 0.2 and antiseptic 0.5.
Described cosolvent is one or more in sodium salicylate, acetamide, tween 80, ethanol, propylene glycol.
Described antioxidant is sodium sulfite, sodium pyrosulfite or ascorbic acid.
Described antiseptic is sodium benzoate, potassium sorbate or benzyl alcohol.
The preparation method concrete steps of described Poria injection are as follows:
(1) Poria is dry, pulverizing, excessively 40 ~ 60 mesh sieves obtain Indian Bread;
(2) purified water being added 1mol/L sodium hydroxide solution regulates purified water pH value to be 9 ~ 11 obtain alkaline purified water;
(3) step (1) formula ratio Indian Bread is taken, add 8 ~ 10 times of alkaline purified water of step (2), extract 3 times, each 1 ~ 2h, merge three extracting solution, filtering and concentrating obtains mixture a to Poria formula ratio 0.5 ~ 1.5 times, dehydrated alcohol is added to alcohol content 55%(V/V) in mixture a, leave standstill, elimination supernatant, obtains mixture b by precipitate purified water standardize solution;
(4) in step (3) mixture b, adding formula ratio cosolvent, antioxidant and antiseptic, boil dissolving, then cool, is 5.5 ~ 7.0 with 0.1mol/L hydrochloric acid solution adjust ph after cooling, standardize solution, filtration, fill, sterilizing and get final product.
compared with prior art, the present invention has following technique effect:
Pachyman is the principle active component of Chinese herbal medicine Poria, energy invigorating the spleen and benefiting QI, and immunity moderation function regulating YIN and YANG balances, and maintains the good body constitution of body.Pachyman product commercially available at present mainly contains oral liquid, pulvis and granule, is oral formulations, and wherein pulvis and granule are mainly based on compound product, and the pachyman injection product of folk prescription not yet occurs.The present invention adopts single prescription Poria to prepare Poria injection, and preparation technology is simple, is easy to realize.The present invention is injection, intramuscular administration, and the pathological symptom of energy rapid recovery animal, improves immunocompetence and the bioavailability of medicine in body of animal.
Usage and dosage: intramuscular injection, dosage is 0.1 ml/kg, 1 ~ 3 time/d, within 3rd ~ 7, is a course for the treatment of.
Detailed description of the invention
Below in conjunction with embodiment, the present invention is further described, but do not limit content of the present invention.
embodiment 1
A kind of Poria injection, is made up of the raw material of following weight portion: Poria 80 ~ 120, cosolvent 0.5 ~ 30, antioxidant 0.1 ~ 0.5 and antiseptic 0.4 ~ 0.8.
Described cosolvent is one or more in sodium salicylate, acetamide, tween 80, ethanol, propylene glycol.
Described antioxidant is sodium sulfite, sodium pyrosulfite or ascorbic acid.
Described antiseptic is sodium benzoate, potassium sorbate or benzyl alcohol.
The preparation method concrete steps of described Poria injection are as follows:
(1) Poria is dry, pulverizing, excessively 40 ~ 60 mesh sieves obtain Indian Bread;
(2) purified water being added 1mol/L sodium hydroxide solution regulates purified water pH value to be 9 ~ 11 obtain alkaline purified water;
(3) step (1) formula ratio Indian Bread is taken, add 8 ~ 10 times of alkaline purified water of step (2), extract 3 times, each 1 ~ 2h, merge three extracting solution, filtering and concentrating obtains mixture a to Poria formula ratio 0.5 ~ 1.5 times, dehydrated alcohol is added to alcohol content 55%(V/V) in mixture a, leave standstill, elimination supernatant, obtains mixture b by precipitate purified water standardize solution;
(4) in step (3) mixture b, adding formula ratio cosolvent, antioxidant and antiseptic, boil dissolving, then cool, is 5.5 ~ 7.0 with 0.1mol/L hydrochloric acid solution adjust ph after cooling, standardize solution, filtration, fill, sterilizing and get final product.
embodiment 2
A kind of Poria injection, is made up of the raw material of following weight portion: Poria 100g, sodium salicylate 5g, sulfurous acid acid sodium 0.1g and benzyl alcohol 0.4g.
The preparation method of the present embodiment is identical with the preparation method of embodiment 1.
embodiment 3
A kind of Poria injection, is made up of the raw material of following weight portion: Poria 80g, sodium salicylate 5g, tween 80 5g, sodium pyrosulfite 0.1g and benzyl alcohol 0.7g.
The preparation method of the present embodiment is identical with the preparation method of embodiment 1.
embodiment 4
A kind of Poria injection, is made up of the raw material of following weight portion: Poria 90g, acetamide 6g, propylene glycol 10g, sodium sulfite 0.15g and potassium sorbate 0.6g.
The preparation method of the present embodiment is identical with the preparation method of embodiment 1.
embodiment 5
A kind of Poria injection, is made up of the raw material of following weight portion: Poria 110g, acetamide 2g, propylene glycol 15g, sodium pyrosulfite 0.2g and sodium benzoate 0.5g.
The preparation method of the present embodiment is identical with the preparation method of embodiment 1.
embodiment 6
A kind of Poria injection, is made up of the raw material of following weight portion: Poria 120g, ethanol 5g, propylene glycol 15g, ascorbic acid 0.4g and benzyl alcohol 0.5g.
The preparation method of the present embodiment is identical with the preparation method of embodiment 1.
experimental example
Choose health, ablactational baby pig 42 (25 age in days) that body weight is consistent, be divided into 6 groups at random, often organize 7, public, ratio is consistent, weaning pig feeding standard of feeding daily ration, separated raising, each feedstuff addition with in pit of having enough slightly clout for degree, freely drink water.Matched group: conventional raising; Test 1 group: Poria injection prepared by intramuscular injection embodiment 2, dosage is 0.1 ml/kg, 1 time/d; Test 2 groups: Poria injection prepared by intramuscular injection embodiment 3, dosage is 0.1 ml/kg, 1 time/d; Test 3 groups: Poria injection prepared by intramuscular injection embodiment 4, dosage is 0.1 ml/kg, 1 time/d; Test 4 groups: Poria injection prepared by intramuscular injection embodiment 5, dosage is 0.1 ml/kg, 1 time/d; Test 5 groups: Poria injection prepared by intramuscular injection embodiment 6, dosage is 0.1 ml/kg, 1 time/d.
Raise after 7 days, test pig adopts vena cava anterior blood sampling, blood serum sample is obtained from 15m in through 3000r/m in,-20 DEG C save backup, for detecting the content (immunoturbidimetry of serum immunoglobulin IgG and IgM, measuring test kit is Beijing Leadman Biochemistry Technology Co., Ltd.'s product), the results are shown in Table 1.
As shown in Table 1, ablactational baby pig injection Poria injection is after 1 week, and the content of the immunoglobulin in its blood is significantly higher than the test pig not using pachyman injection.Visible, the present invention prepares the immunity that pachyman injection can improve animal, reduces Animal diseases and occurs.
Claims (7)
1. a Poria injection, is characterized in that: be made up of the raw material of following weight portion: Poria 80 ~ 120, cosolvent 0.5 ~ 30, antioxidant 0.1 ~ 0.5 and antiseptic 0.4 ~ 0.8.
2. Poria injection according to claim 1, is characterized in that: be made up of the raw material of following weight portion: Poria 90 ~ 110, cosolvent 15 ~ 25, antioxidant 0.2 ~ 0.4 and antiseptic 0.5 ~ 0.6.
3. Poria injection according to claim 1, is characterized in that: be made up of the raw material of following weight portion: Poria 100, cosolvent 20, antioxidant 0.2 and antiseptic 0.5.
4. Poria injection according to any one of claims 1 to 3, is characterized in that: described cosolvent is one or more in sodium salicylate, acetamide, tween 80, ethanol, propylene glycol.
5. Poria injection according to any one of claims 1 to 3, is characterized in that: described antioxidant is sodium sulfite, sodium pyrosulfite or ascorbic acid.
6. Poria injection according to any one of claims 1 to 3, is characterized in that: described antiseptic is sodium benzoate, potassium sorbate or benzyl alcohol.
7. the preparation method of Poria injection according to any one of claims 1 to 3, is characterized in that: concrete steps are as follows:
(1) Poria is dry, pulverizing, excessively 40 ~ 60 mesh sieves obtain Indian Bread;
(2) purified water being added 1mol/L sodium hydroxide solution regulates purified water pH value to be 9 ~ 11 obtain alkaline purified water;
(3) step (1) formula ratio Indian Bread is taken, add 8 ~ 10 times of alkaline purified water of step (2), extract 3 times, each 1 ~ 2h, merge three extracting solution, filtering and concentrating obtains mixture a to Poria formula ratio 0.5 ~ 1.5 times, dehydrated alcohol is added to alcohol content 55%(V/V) in mixture a, leave standstill, elimination supernatant, obtains mixture b by precipitate purified water standardize solution;
(4) in step (3) mixture b, adding formula ratio cosolvent, antioxidant and antiseptic, boil dissolving, then cool, is 5.5 ~ 7.0 with 0.1mol/L hydrochloric acid solution adjust ph after cooling, standardize solution, filtration, fill, sterilizing and get final product.
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Cited By (1)
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CN111419886A (en) * | 2020-04-20 | 2020-07-17 | 劲牌持正堂药业有限公司 | Preparation method of poria cocos formula granules |
Citations (4)
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CN111419886A (en) * | 2020-04-20 | 2020-07-17 | 劲牌持正堂药业有限公司 | Preparation method of poria cocos formula granules |
CN111419886B (en) * | 2020-04-20 | 2022-09-30 | 劲牌持正堂药业有限公司 | Preparation method of poria cocos formula granules |
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