CN104488404A - Priming method for promoting seeds of marsdenia tenacissima to germinate under salt stress - Google Patents
Priming method for promoting seeds of marsdenia tenacissima to germinate under salt stress Download PDFInfo
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- CN104488404A CN104488404A CN201410815059.6A CN201410815059A CN104488404A CN 104488404 A CN104488404 A CN 104488404A CN 201410815059 A CN201410815059 A CN 201410815059A CN 104488404 A CN104488404 A CN 104488404A
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- salt stress
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Abstract
The invention relates to a priming method for promoting seeds of marsdenia tenacissima to germinate under salt stress. The germination rate, the germination potential and the germination index of the seeds of the marsdenia tenacissima under the salt stress can be remarkably increased once the seeds of marsdenia tenacissima are primed for 12 hours by using a 300mg/L GA3 solution, wherein the germination rate is increased from 52.67% to 80.67%, the germination potential is increased from 24.00% to 36.67% and the germination index is increased from 7.43 to 10.95. The anti-oxide enzyme activity is remarkably enhanced and the stress resistance of the seeds of the marsdenia tenacissima under the salt stress is remarkably enhanced. In addition, the method is simple, easy to operate, low in cost, free of environment pollution and suitable for actual production and application.
Description
Technical field
The present invention relates to the initiating method sprouted under promoting CAULIS MARSDENIAE TENACISSIMAE seed salt stress, belong to technical field of Chinese herbal medicine cultivation.
Background technology
CAULIS MARSDENIAE TENACISSIMAE Marsdenia tenocissima (Roxb) Wight et Arn. is Asclepiadaceae Marsdenia plant, and be used as medicine with rattan, medicinal material is called CAULIS MARSDENIAE TENACISSIMAE, have clearing heat and detoxicating, protect the liver diuresis, the effects such as eliminating stagnation to stop pain.CAULIS MARSDENIAE TENACISSIMAE is used to treat clinical various cancer widely in recent years, and annual medicinal material demand is comparatively large, and only can not meet supply by wild purchase, large area artificial cultivation will become a development trend of CAULIS MARSDENIAE TENACISSIMAE.
China is that soil endangers one of heavier country by salinization of soil, one of the major stress factors existing for restriction crop growth in a large amount of saline land.Plant seed is responsive to salinity in the sprouting stage, under condition of salt stress, and Seed germination suffers.Can seed healthy seedling be the prerequisite of vine growth and development under condition of salt stress.GA
3be a kind of biological regulation material, the generation of energy inducing plant body endoenzyme, the degraded of catalysis reserve substance, thus the metabolism promoting seed germination, especially have certain facilitation for the seed germination under salt adverse circumstance.GA
3vegetable crop, cereal crops and rare medicinal plant are mainly concentrated on to higher plant Effect study.Because CAULIS MARSDENIAE TENACISSIMAE primary growth is in karst landform district, it is more responsive to soil salt, and does not almost have the research of CAULIS MARSDENIAE TENACISSIMAE seed at present, and the application of seed priming has no report especially.
The invention provides a kind of promote CAULIS MARSDENIAE TENACISSIMAE seed salt stress under the initiating method sprouted, germination rate and the germination index of seed can be improved, and increase its salt resistance.For improving the service efficiency of CAULIS MARSDENIAE TENACISSIMAE seed, the standardized cultivation realizing CAULIS MARSDENIAE TENACISSIMAE is significant, is GA simultaneously
3application on medicinal plant and the sprouting of promotion seed under condition of salt stress provide certain technical support.
Summary of the invention
The object of the present invention is to provide a kind of initiating method that CAULIS MARSDENIAE TENACISSIMAE Marsdenia tenocissima (Roxb) Wight et Arn. seed is sprouted under salt stress that promotes, the germination rate of seed is made to be increased to 80.67% from 52.67%, germination index is increased to 10.95 from 7.43, activities of antioxidant enzymes significantly raises, and salt resistance significantly strengthens.
Provided by the present invention a kind of promote CAULIS MARSDENIAE TENACISSIMAE seed salt stress under the initiating method sprouted, comprise:
A: seed collection and process: for subsequent use in 4 ± 0.5 DEG C of Refrigerator stores after CAULIS MARSDENIAE TENACISSIMAE seed being removed impurity and flat grain;
B: seed pre-treatment: choose full seed clean water 2h, get the seed of full sinking, pull out, dry the surface of the seed moisture on blotting paper;
C: gibberellin causes: under 30 DEG C of constant temperature dark conditions, seed is placed in 300mgL
-1gA
3cause 12h in solution, take out, running water running water 5min, blotting paper blots surface moisture, is evenly spread out on clean filter paper and dries to original dry seeds weight;
D: seed germination: the seed after causing Hui Gan is placed in the thick moistening fine sand of 4cm (sand and salting liquid volume ratio are 4: 1), in every germinating bed 100, cultivation temperature 30 DEG C, light intensity 3300lx, light dark period 16h/8h, duration of germination, sprays appropriate running water at regular time and quantity, and seed is sprouted through 2 ~ 10d.
The invention has the advantages that:
Pass through GA
3priming Seeds, germination rate and germination vigor and the germination index etc. of CAULIS MARSDENIAE TENACISSIMAE seed under salt stress can be significantly improved, the germination rate of its seed is made to be increased to 80.67% from 52.67%, germination index is increased to 10.95 from 7.43, activities of antioxidant enzymes significantly raises, and the salt resistance of CAULIS MARSDENIAE TENACISSIMAE seed is significantly strengthened.In addition, the method is simple, and be easy to operation, cost is low, environmentally safe, is suitable for production application.
Accompanying drawing explanation
Fig. 1 GA
3solution causes and does not process CAULIS MARSDENIAE TENACISSIMAE seedling situation with causing
Embodiment
Test example 1
A: seed collection and process: for subsequent use in 4 ± 0.5 DEG C of Refrigerator stores after CAULIS MARSDENIAE TENACISSIMAE seed being removed impurity and flat grain;
B: seed pre-treatment: choose full seed clean water 2h, get the seed of full sinking, pull out, dry the surface of the seed moisture on blotting paper;
C: gibberellin causes: seed is placed in 100 under 30 DEG C of constant temperature dark conditions, 200,300,400,500mgL
-1gA
3cause 12h in solution, take out, running water running water 5min, blotting paper blots surface moisture, is evenly spread out on clean filter paper and dries to original dry seeds weight;
D: seed germination: the seed after causing Hui Gan is placed in the thick moistening fine sand of 4cm (sand and 0.8% salting liquid volume ratio are 4: 1), in every germinating bed 100, cultivation temperature 30 DEG C, light intensity 3300lx, light dark period 16h/8h, duration of germination, sprays appropriate running water at regular time and quantity, and seed is sprouted through 2 ~ 10d.
A. percentage of seedgermination statistics: number of days when stretching out 2mm using seed rhizome place plumule is as first gate time, and reach the highest with seed germination number, number of days when later occurring without germination seed is again as last gate time.Add up germination vigor during 4d, add up germination rate, germination index during 10d, rhizome is long and divide radical.
B. seedling antioxidation activity enzymatic determination: seed sprouting 10d, random selecting respectively processes the consistent fresh measuring blade of growth site.Superoxide dismutase (SOD) activity adopts nitroblue tetrazolium (NBT) method to measure; Catalase (CAT) activity adopts ultraviolet absorption method to measure; Malonaldehyde (MDA) content adopts thiobarbituricacidα-(TBA) method to measure; Soluble protein content adopts Coomassie brilliant G-250 method to measure.
Result shows, the GA of debita spissitudo
3after solution causes process, germination rate, the germination vigor and germination index etc. of CAULIS MARSDENIAE TENACISSIMAE seed significantly improve (table 1), illustrate that causing process significantly promotes the growth of CAULIS MARSDENIAE TENACISSIMAE seedling under condition of salt stress, significantly can alleviate the injury of salt stress to CAULIS MARSDENIAE TENACISSIMAE seedling.GA
3activities of antioxidant enzymes, the soluble protein content of the rear CAULIS MARSDENIAE TENACISSIMAE seedling of solution initiation process are significantly increased; mda content significantly reduces (table 2); illustrate that causing process significantly improves CAULIS MARSDENIAE TENACISSIMAE seedling defence enzyme activity; reduce the degree of damaged membrane wound, thus CAULIS MARSDENIAE TENACISSIMAE salt resistance is significantly strengthened.
Table 1 GA
3cAULIS MARSDENIAE TENACISSIMAE seed germination situation under solution-treated
Table 1 Seed germination of M.tenocissima by GA
3priming
Note: the different lowercase alphabet of same column shows significant difference, P < 0.05.Lower same.
Table 2 GA
3solution-treated is on the impact of activities of antioxidant enzymes and MDA and soluble protein content in CAULIS MARSDENIAE TENACISSIMAE seedling leaves
Table 2 Effects of GA
3priming on activity of SOD，CAT and content of MDA and soluble protein
Analyzed from table one, work as GA
3concentration is 300mgL
-1time, the germination vigor of CAULIS MARSDENIAE TENACISSIMAE seed is peak 36.67%, and germination rate is 80.67%, and germination index is 10.95, and rhizome length and a point radical all compare and significantly improve according to CK.As shown in Table 2, GA is worked as
3concentration is 300mgL
-1time, SOD activity is that 444.61, CAT and soluble protein content also significantly increase.
Specific embodiment 1
A: seed collection and process: for subsequent use in 4 ± 0.5 DEG C of Refrigerator stores after CAULIS MARSDENIAE TENACISSIMAE Marsdenia tenacissima (Roxb.) Wight et Arn. seed is removed impurity and flat grain;
B: seed pre-treatment: choose full seed clean water 2h, get the seed of full sinking, pull out, dry the surface of the seed moisture on blotting paper;
C: gibberellin causes: under 30 DEG C of constant temperature dark conditions, seed is placed in 300mgL
-1gA
3cause 12h in solution, take out, running water running water 5min, blotting paper blots surface moisture, is evenly spread out on clean filter paper and dries to original dry seeds weight;
D: seed germination: the seed after causing Hui Gan is placed in the thick moistening fine sand of 4cm (sand and 0.8% salting liquid volume ratio are 4: 1), in every germinating bed 100, cultivation temperature 30 DEG C, light intensity 3300lx, light dark period 16h/8h, duration of germination, spray appropriate running water at regular time and quantity, seed is sprouted through 2 ~ 10d, and germination rate can reach 80.67%, and germination index reaches 10.95.
Claims (2)
1. the initiating method sprouted under promoting CAULIS MARSDENIAE TENACISSIMAE Marsdenia tenacissima Roxb.Wight et Arn. seed salt stress, comprises the following step:
A: seed collection and process: for subsequent use in 4 ± 0.5 DEG C of Refrigerator stores after CAULIS MARSDENIAE TENACISSIMAE seed being removed impurity and flat grain;
B: seed pre-treatment: choose full seed clean water 2h, get the seed of full sinking, pull out, dry the surface of the seed moisture on blotting paper;
C: gibberellin causes: under 30 DEG C of constant temperature dark conditions, seed is placed in 300mgL
-1gA
3cause 12h in solution, take out, running water running water 5min, blotting paper blots surface moisture, is evenly spread out on clean filter paper and dries to original dry seeds weight;
D: seed germination: by cause the seed after Hui Gan be placed in 4cm thick, sand and 0.8%NaCl salting liquid volume ratio be 4: 1 moistening fine sand, in every germinating bed 100, cultivation temperature 30 DEG C, light intensity 33001x, light dark period 16h/8h, duration of germination, sprays appropriate running water at regular time and quantity, and seed is sprouted through 2 ~ 10d.
2. according to claim 1 a kind of promote CAULIS MARSDENIAE TENACISSIMAE seed salt stress under the method sprouted, it is characterized in that: under salt stress, germination rate is that the CAULIS MARSDENIAE TENACISSIMAE seed of 52.67% is through 300mgL
-1gA
3after causing 12h in solution, in 10d, germination rate rises to 80.67%, and germination index is increased to 10.95 from 7.43, and activities of antioxidant enzymes significantly raises, and germination rate and salt resistance significantly strengthen.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107333550A (en) * | 2017-08-03 | 2017-11-10 | 云南信通植物药业有限公司 | A kind of CAULIS MARSDENIAE TENACISSIMAE seed seedling-raising method |
Citations (5)
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|---|---|---|---|---|
| WO2003045132A1 (en) * | 2001-11-26 | 2003-06-05 | Council Of Scientific And Industrial Research | Media compositions for faster propagation of swertia chirata |
| CN101385412A (en) * | 2008-09-26 | 2009-03-18 | 云南省烟草科学研究所 | Method for reducing germinating time of tobacco coating pilled seeds |
| CN102550165A (en) * | 2012-03-05 | 2012-07-11 | 南京农业大学 | Induction method for promoting germination of Oldenlandia diffusa seeds |
| CN103109620A (en) * | 2012-11-16 | 2013-05-22 | 高润宝 | Osmoregulation initiation method of corn seeds under salt stress |
| CN103109847A (en) * | 2012-11-16 | 2013-05-22 | 高润宝 | Sorghum seed initiator under saline-alkali stress |
-
2014
- 2014-12-22 CN CN201410815059.6A patent/CN104488404A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2003045132A1 (en) * | 2001-11-26 | 2003-06-05 | Council Of Scientific And Industrial Research | Media compositions for faster propagation of swertia chirata |
| CN101385412A (en) * | 2008-09-26 | 2009-03-18 | 云南省烟草科学研究所 | Method for reducing germinating time of tobacco coating pilled seeds |
| CN102550165A (en) * | 2012-03-05 | 2012-07-11 | 南京农业大学 | Induction method for promoting germination of Oldenlandia diffusa seeds |
| CN103109620A (en) * | 2012-11-16 | 2013-05-22 | 高润宝 | Osmoregulation initiation method of corn seeds under salt stress |
| CN103109847A (en) * | 2012-11-16 | 2013-05-22 | 高润宝 | Sorghum seed initiator under saline-alkali stress |
Non-Patent Citations (1)
| Title |
|---|
| 孟衡玲 等: "药用植物通关藤种子的生物学特性研究", 《种子》, vol. 33, no. 7, 31 July 2014 (2014-07-31) * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107333550A (en) * | 2017-08-03 | 2017-11-10 | 云南信通植物药业有限公司 | A kind of CAULIS MARSDENIAE TENACISSIMAE seed seedling-raising method |
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