CN104483253A - Micro device used for studying egg cells and microscope system - Google Patents
Micro device used for studying egg cells and microscope system Download PDFInfo
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- CN104483253A CN104483253A CN201510016969.2A CN201510016969A CN104483253A CN 104483253 A CN104483253 A CN 104483253A CN 201510016969 A CN201510016969 A CN 201510016969A CN 104483253 A CN104483253 A CN 104483253A
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Abstract
The invention provides a micro device used for studying egg cells. The micro device used for studying egg cells comprises an egg cell micro-irrigation unit and a circulation refrigeration unit. The egg cell micro-irrigation unit comprises a first covering plate and a second covering plate which are used for fixing the egg cell micro-irrigation unit. The circulation refrigeration unit is tightly close to the first covering plate, a first film and a second film are sequentially arranged between the first covering plate and the second covering plate, and a micro-irrigation cavity is formed by a first curved groove in the first film and a second curved groove in the second film; the micro-irrigation cavity is provided with an inlet guide pipe and an outlet guide pipe, and temperature sensing equipment is arranged in the cavity; a fixing baffle which is used for fixing the positions of egg cells and permits flowing of a solution is arranged at the position, between the inlet guide pipe and the outlet guide pipe, in the cavity. The invention further provides a microscope system, after the micro device is connected with an irrigation microscope device, the formed microscope system can be used for measuring the size response of egg cells subjected to changes of the concentration at a specific temperature, and then the permeability parameter of the cell membrane is determined.
Description
Technical field
The application belongs to Cryomedicine technical field, is specifically related to a kind of microdevice for studying egg cell and microscopic system.
Background technology
In recent years, the incidence of infertility improves increasingly, and traditional medicine and therapeutic method of surgery far can not meet the demand of patient to fertility.Issues of Human Assisted Reproductive Technologies is that the healing of infertility provides new way, due to the characteristic of auxiliary procreation technology, unavoidably will relate to the freezen protective of ovum in the process implementing this technology.The birth of the freezen protective technology of egg mother cell is an important breakthrough of biomedicine field, by freezen protective egg mother cell, the women of infertile or several first ovarian resection can be helped to obtain offspring.Therefore, freezen protective egg cell has development prospect widely.
Typical Cord blood process mainly comprises: before (1) Cord blood, the interpolation of cryoprotective agent; (2) in refrigerating process, the Programmed control of rate of temperature fall; After rewarming (3), before Clinical practice, the removal of cryoprotective agent.Carry out the optimal design of these three important steps of Cord blood process, need to measure the important biomolecule physics parameter of cell, volume as isotonic in cell, cell divide permeability parameter to cryoprotective agent and water of perviousness volume, cell membrane and energy of activation thereof etc.
In order to obtain better preservation effect, need to understand the permeability parameter of egg cell in low temperature and solution environmental, but existing perfusion microscope is difficult to the permeability parameter measuring egg cell.
Summary of the invention
In view of this, the application provides a kind of microdevice for studying egg cell and microscopic system, and microdevice described in the application can be the test of egg cell permeability experiment and provides reliable and controlled test environment, thus for studying egg cell perviousness.
The application provides a kind of microdevice for studying egg cell, comprises egg cell micro perfusion unit and circularly cooling unit; Described egg cell micro perfusion unit comprises:
For the first cover plate and second cover plate of fixing micro perfusion unit; Described circularly cooling unit is close to the first cover plate;
The first film and the second film is disposed with between described first cover plate and the second cover plate; Described the first film is provided with the first cutting, described second film is provided with the second cutting, described second cutting and the first cutting form micro perfusion chamber; Described micro perfusion chamber is provided with entry conductor and delivery channel, for perfusion egg cell solution; Temperature sensing device is provided with in described micro perfusion chamber;
Between described entry conductor and delivery channel, in described micro perfusion chamber, be provided with fixed dam; Described fixed dam is used for fixing egg cell position, and allows solution to pass through.
Preferably, the making material of described first cover plate and the second cover plate is independently selected from silex glass.
Preferably, the making material of described the first film and the second film is silica gel.
Preferably, the thickness of described the first film is 10 microns ~ 400 microns; The thickness of described second film is 5 microns ~ 140 microns;
The quantity of described fixed dam is 1; Described fixed dam is arranged on the first film, and has gap between its lower end and the second film.
Preferably, the width in described micro perfusion chamber is 110 microns ~ 5 millimeters; The height that fixed dam place is removed in described micro perfusion chamber is 100 microns ~ 1 millimeter.
Preferably, the making material of described fixed dam is silica gel, and itself and the first film are one-body molded.
Preferably, described entry conductor and delivery channel are the kapillary that internal diameter is 0.3 millimeter ~ 1 millimeter.
Preferably, described circularly cooling unit comprises:
There is the cavity of refrigerating fluid torus; Described cavity two ends are respectively arranged with refrigerating fluid import and refrigerating fluid outlet;
Described cavity is provided with entry conductor through hole and delivery channel through hole, is respectively used to place described entry conductor and delivery channel.
Preferably, the making material of described cavity is dimethyl silicone polymer.
The application also provides a kind of microscopic system, for measuring the permeability parameter of egg cell, comprising:
Microdevice mentioned above;
And the perfusion microscope device to be connected with described microdevice.
Compared with prior art, what the application provided comprises for the microdevice studying egg cell: the egg cell micro perfusion unit be made up of the first cover plate, the second cover plate, the first film, the second film, entry conductor, delivery channel and temperature sensing device etc., and is close to the circularly cooling unit of micro perfusion unit.In the embodiment of the present application, described first cover plate and the second cover plate grip micro perfusion unit; Described the first film and the second film form micro perfusion chamber by cutting.The application loads egg cell to micro perfusion chamber from entry conductor, then injects cryoprotective agent to micro perfusion chamber from entry conductor; Extract cryoprotective agent out from delivery channel, egg cell will be stuck in fixed dam place and be fixed, and due to the protection of egg cell oolemma, fixed dam can not have an impact to the form of egg cell, and solution can continue flowing.During unloading egg cell, inject cryoprotective agent from delivery channel, extract cryoprotective agent from entry conductor, egg cell will be drawn out of along with cryoprotective agent.The application utilizes egg cell oolemma egg cell to be stuck in the fixed position in micro perfusion chamber, and can not affect the morphological feature of egg cell, thus provides stable environment for studying the character of egg cell.Further, the application's circularly cooling unit is used for making liquid in micro perfusion chamber reach specified temp; The temperature of solution residing for egg cell is directly measured by the temperature sensing device being built in micro perfusion chamber.Therefore, the microdevice of the micro perfusion unit and circularly cooling unit formation with micro perfusion chamber can provide controlled, reliable test environment for nature parameters such as the perviousness of egg cell.
After microdevice described in the application is connected with perfusion microscope device, the microscopic system of formation can be used for measuring egg cell at a certain temperature, the volume response after experience concentration change, and then determines the permeability parameter of cell membrane.
Accompanying drawing explanation
In order to be illustrated more clearly in the embodiment of the present invention or technical scheme of the prior art, be briefly described to the accompanying drawing used required in embodiment or description of the prior art below, apparently, accompanying drawing in the following describes is only embodiments of the invention, for those of ordinary skill in the art, under the prerequisite not paying creative work, other accompanying drawing can also be obtained according to the accompanying drawing provided.
The structural representation of the microdevice for studying egg cell that Fig. 1 provides for the embodiment of the present application;
The structural representation of the egg cell micro perfusion unit that Fig. 2 provides for the embodiment of the present application;
The structural representation of the first film and the second film in the egg cell micro perfusion unit that Fig. 3 provides for the embodiment of the present application;
The structural representation of the circularly cooling unit that Fig. 4 provides for the embodiment of the present application;
The diagrammatic cross-section of the circularly cooling unit that Fig. 5 provides for the embodiment of the present application;
Egg cell volume continually varying photo after interpolation 1mol/L ethylene glycol that Fig. 6 provides for the embodiment of the present application.
Embodiment
Below in conjunction with the accompanying drawing in the embodiment of the present invention, be clearly and completely described the technical scheme in the embodiment of the present invention, obviously, described embodiment is only the present invention's part embodiment, instead of whole embodiments.Based on the embodiment in the present invention, those of ordinary skill in the art, not making the every other embodiment obtained under creative work prerequisite, belong to the scope of protection of the invention.
This application provides a kind of microdevice for studying egg cell, comprising egg cell micro perfusion unit and circularly cooling unit; Described egg cell micro perfusion unit comprises:
For the first cover plate and second cover plate of fixing micro perfusion unit; Described circularly cooling unit is close to the first cover plate;
The first film and the second film is disposed with between described first cover plate and the second cover plate; Described the first film is provided with the first cutting, described second film is provided with the second cutting, described second cutting and the first cutting form micro perfusion chamber; Described micro perfusion chamber is provided with entry conductor and delivery channel, for perfusion egg cell solution; Temperature sensing device is provided with in described micro perfusion chamber;
Between described entry conductor and delivery channel, in described micro perfusion chamber, be provided with fixed dam; Described fixed dam is used for fixing egg cell position, and allows solution to pass through.
The invention provides one and be used for research egg cell character specially as infiltrative microdevice, egg cell position can be fixed easily, and control solution environmental residing for egg cell and temperature.
See the structural representation of the microdevice for studying egg cell that Fig. 1, Fig. 1 provide for the embodiment of the present application.In Fig. 1,1 is egg cell micro perfusion unit, and 11 is the first cover plate, and 12 is the second cover plate, and 13 is the first film, and 14 is the second film, and 15 is entry conductor, and 16 is delivery channel, and 17 is thermopair; 2 is circularly cooling unit, and 21 is cavity, and 22 is refrigerating fluid import, and 23 is refrigerating fluid outlet.
The infiltrative device of research egg cell that can be used for disclosed by the invention comprises: egg cell micro perfusion unit 1.Ovum is spherical, and people's mature egg cell dia is approximately 120 microns, and its size (100 microns ~ 150 microns) is much larger than general cell size (10 microns ~ 20 microns); Further, whole egg cell outermost layer is enclosed with layer of transparent ribbon (abbreviation oolemma)---and glycoprotein, this material had both had the effect of protection egg cell, can catch again and limit sperm, also making egg cell not adherent.Therefore, egg cell micro perfusion unit 1 of the present invention can hold egg cell dimensionally, also can fix egg cell in flow process, thus measure its permeability parameter etc.
In this application, egg cell micro perfusion unit 1 comprises the first cover plate 11 and the second cover plate 12, for fixing micro perfusion unit; Also upper wall 11 and lower wall 12 can be called.In the embodiment of the present application, described first cover plate and the second cover plate be arranged in parallel, and two ends can be closed, and also can not close; Size is preferably identical, and the application does not have other particular restriction.The making material of described first cover plate and the second cover plate is preferably independently selected from silex glass, and heat conduction is good, is easy to observe.In an embodiment of the application, described first cover plate and the second cover plate are parallel two-layer silex glass, by gripping double-layer films.
Between the first cover plate 11 and the second cover plate 12, the present invention sets gradually the first film 13 and the second film 14.As preferably, the making material of described the first film and the second film is silica gel.In the present invention, described film is two notched films, coordinates formation enclosed cavity, namely micro perfusion chamber by the cutting on it.Namely described the first film is provided with the first cutting, described second film is provided with the second cutting, described second cutting and the first cutting form micro perfusion chamber.
In the present invention, described micro perfusion chamber is provided with entry conductor 15 and delivery channel 16, and the kapillary that this area can be adopted conventional is as conduit.As preferably, described entry conductor and delivery channel are the kapillary that internal diameter is 0.3 millimeter ~ 1 millimeter.The setting of the present invention to described conduit is not particularly limited, can be used for load, unloading egg cell and injection, extraction solution, realize perfusion egg cell solution.In an embodiment of the application, entry conductor 15 is used for loading, unloading egg cell and inject solution; Delivery channel 16 is used for extracting out, injecting solution.During the embodiment of the present application perfusion egg cell, load egg cell to micro perfusion chamber from entry conductor 15, then inject the conventional cryoprotective agent in this area to micro perfusion chamber from entry conductor 15, extract cryoprotective agent out from delivery channel 16; During unloading egg cell, inject cryoprotective agent from delivery channel 16, extract cryoprotective agent from entry conductor 15, egg cell will be drawn out of along with cryoprotective agent.
Temperature sensing device is provided with, thermopair 17 as shown in Figure 1, to monitor the temperature in micro perfusion chamber in described micro perfusion chamber.The present invention is not particularly limited described temperature sensing device, and as in one embodiment of the invention, delivery channel 16 pressed close to by thermopair 17; Thermopair 17 is inserted in micro perfusion chamber by the form of bending completely, in real time, directly can monitor the temperature of solution environmental residing for egg cell.
See the structural representation of the egg cell micro perfusion unit that Fig. 2, Fig. 2 provide for the embodiment of the present application.In Fig. 2,11 is the first cover plate, and 12 is the second cover plate, and 13 is the first film, and 14 is the second film, and 15 is entry conductor, and 16 is delivery channel, and 17 is thermopair, and 18 is micro perfusion chamber, and 19 is fixed dam.
Between the entry conductor 15 and delivery channel 16 of the embodiment of the present application, in micro perfusion chamber 18, be provided with fixed dam 19.Described fixed dam is used for fixing egg cell position, and allows solution to pass through.
The embodiment of the present application loads egg cell to micro perfusion chamber 18 from entry conductor 15, then injects cryoprotective agent to micro perfusion chamber 18 from entry conductor 15; Extract cryoprotective agent out from delivery channel 16, egg cell will be stuck in fixed dam 19 place and be fixed, and due to the protection of egg cell oolemma, fixed dam 19 can not have an impact to the form of egg cell, and solution can continue flowing.During unloading egg cell, inject cryoprotective agent from delivery channel 16, extract cryoprotective agent from entry conductor 15, egg cell will be drawn out of along with cryoprotective agent.The application utilizes egg cell oolemma egg cell to be stuck in the fixed position in micro perfusion chamber, and can not affect the morphological feature of egg cell, thus provides stable environment for studying the character of egg cell.
In one embodiment of the invention, the quantity of described fixed dam is 1, can be arranged on arbitrary film.As shown in Figure 2, in micro perfusion chamber 18, fixed dam 19 is arranged on the first film 13, and has gap between its lower end and the second film 14.Accordingly, described fixed dam also can be arranged on the second film, and has gap between its lower end and the first film.In addition, the quantity of described fixed dam can also be 2, is separately positioned on the same position of the first film and the second film, and has gap between two fixed dams.The size of the embodiment of the present invention to described gap is not particularly limited, and solution can be allowed to pass through and do not allow egg cell to pass through, and it is preferably dimensioned to be 1/2 ~ 1/3 of egg cell diameter.
In this application, described fixed dam can be arranged perpendicular to film, also can be obliquely installed; The shape of the application to described fixed dam is also not particularly limited.The making material of described fixed dam is preferably silica gel; Further, fixed dam described in the embodiment of the present application can be one-body molded with the first film.
The structural representation of the first film and the second film in the egg cell micro perfusion unit provided for the embodiment of the present application see Fig. 3, Fig. 3.In Fig. 3,13 is the first film, and 14 is the second film, and 19 is fixed dam; 131 is the first short groove, and 132 is the second short groove, and 141 is the second cutting.
In an embodiment of the application, fixed dam 19 is arranged on the first film 13, and the first cutting on the first film 13 is divided into two short grooves, i.e. the first short groove 131 and the second short groove 132; Part in other words conj.or perhaps between the first short groove 131 and the second short groove 132 forms fixed dam 19.And the second film 14 is carved with elongated slot, i.e. the second cutting 141; The outer ledge alignment of elongated slot and short groove, width is identical, and position overlaps, and forms micro perfusion chamber.In the embodiment of the present application, fixed dam 19 is arranged on the first film 13, fixing have gap between plate washer 19 lower end and the second film 14, also micro perfusion chamber is divided into two parts: the first short groove 131 at entry conductor place and the second cutting 141 form the materials chamber of loading egg cell; Laying temperature sensing equipment is convenient in the chamber that the second short groove 132 at delivery channel place and the second cutting 141 are formed, can directly monitor temperature in real time.
Consider that egg cell diameter is the factor such as to arrange of 100 microns ~ 150 microns and fixed dam, in this application, described the first film can be the film being carved with two short grooves, and its thickness is preferably 10 microns ~ 400 microns, is more preferably 20 microns ~ 300 microns; Described second film can be the film being carved with elongated slot, and its thickness is preferably 5 microns ~ 140 microns, is more preferably 8 microns ~ 120 microns.The width in described micro perfusion chamber is preferably 110 microns ~ 5 millimeters, is more preferably 120 microns ~ 4 millimeters; The height that fixed dam place is removed in described micro perfusion chamber is preferably 100 microns ~ 1 millimeter.In an embodiment of the application, the length of the first short groove 131 can be 110 microns ~ 20 millimeters; The length of the second short groove 132 can be greater than 0 and be less than 20 millimeters; The length of the second cutting 141 can be 1 millimeter ~ 60 millimeters, is preferably 5 millimeters ~ 50 millimeters.
As shown in Figure 1, except egg cell micro perfusion unit 1, the infiltrative device of research egg cell that can be used for disclosed by the invention comprises: circularly cooling unit 2.Described circularly cooling unit is close to the first cover plate, is used for making liquid in micro perfusion chamber reach specified temp.
Concrete, the present invention is not particularly limited described circularly cooling unit, preferably includes:
There is the cavity 21 of refrigerating fluid torus; Described cavity two ends are respectively arranged with refrigerating fluid import and refrigerating fluid outlet, refrigerating fluid import 22 as shown in Figure 1 and refrigerating fluid outlet 23;
Described cavity is provided with entry conductor through hole and delivery channel through hole, is respectively used to place described entry conductor and delivery channel.
See the structural representation of the circularly cooling unit that Fig. 4 and Fig. 5, Fig. 4 provide for the embodiment of the present application, the diagrammatic cross-section of the circularly cooling unit that Fig. 5 provides for the embodiment of the present application.In Fig. 4 and Fig. 5,21 is cavity, and 22 is refrigerating fluid import, and 23 is refrigerating fluid outlet, and 24 is entry conductor through hole, and 25 is delivery channel through hole, and 26 is refrigerating fluid torus.
The circularly cooling unit that the embodiment of the present application provides comprises cavity 21, and it has refrigerating fluid torus 26, and two ends are respectively arranged with refrigerating fluid import 22 and refrigerating fluid outlet 23.In the embodiment of the present application, cavity 21 is provided with entry conductor through hole 24 and delivery channel through hole 25, is respectively used to place micro perfusion chamber entry conductor 15 and delivery channel 16.
Described circularly cooling unit can adopt equipment well known to those skilled in the art; As preferably, the making material of described cavity is dimethyl silicone polymer.In the embodiment of the present application, refrigerating fluid import 22 and refrigerating fluid outlet 23 can be connected respectively to the entrance and exit of the liquid refrigerating temperature controlling instrumentss such as low temperature bath, and by the feedback temperature of thermopair 17, the liquid controlling micro perfusion chamber reaches specified temp.Therefore, the microdevice of the micro perfusion unit and circularly cooling unit formation with micro perfusion chamber can provide controlled, reliable test environment for nature parameters such as the perviousness of egg cell.
Present invention also provides a kind of microscopic system, for measuring the permeability parameter of egg cell, comprising:
Microdevice mentioned above;
And the perfusion microscope device to be connected with described microdevice.
After microdevice described in the application is connected with perfusion microscope device, the microscopic system of formation can be used for measuring egg cell at a certain temperature, the volume response after experience concentration change, and then determines the permeability parameter of cell membrane.
Wherein, the content of described microdevice, with consistent above, does not repeat them here.Described perfusion microscope device is the equipment that this area is commonly used, and can be used for the perviousness character measuring egg cell.
According to method assembling microdevice well known to those skilled in the art and microscopic system, the course of work of the embodiment of the present application microscopic system is as follows:
Load egg cell to micro perfusion chamber from entry conductor, then inject cryoprotective agent to micro perfusion chamber from entry conductor; Extract cryoprotective agent out from delivery channel, egg cell will be stuck in fixed dam place and be fixed, and due to the protection of egg cell oolemma, fixed dam can not have an impact to the form of egg cell, and solution can continue flowing.Meanwhile, the application's circularly cooling unit is used for making liquid in micro perfusion chamber reach specified temp; The temperature of solution residing for egg cell is directly measured by the temperature sensing device being built in micro perfusion chamber.Perfusion microscope device makes egg cell at a certain temperature, and experience concentration change also measures its volume response situation, and then determine the permeability parameter of cell membrane.
During unloading egg cell, inject cryoprotective agent from delivery channel, extract cryoprotective agent from entry conductor, egg cell will be drawn out of along with cryoprotective agent.
In order to further illustrate the application, below in conjunction with embodiment, a kind of microdevice and microscopic system for studying egg cell that the application provides being described particularly, but they can not be interpreted as the restriction to the application's protection domain.
Embodiment
For studying the structure of the microdevice of egg cell see Fig. 1 to Fig. 5, wherein, upper wall (the first cover plate) 11 and lower wall (the second cover plate) 12 are silex glass; The first film 13 and the second film 14 are silica gel thin film, and thickness is followed successively by 100 microns, 50 microns.The first film 13 is carved with two short groove 131 and 132, second films 14 and is carved with elongated slot (the second cutting) 141.The width of elongated slot and two short grooves is 200 microns, and length is respectively 44 millimeters, 30 millimeters, 15 millimeters, and outer ledge aligns.First short groove 131 and elongated slot 141 formation are highly the materials chamber of 150 microns, and the part between two short grooves forms baffle plate 19, and it is highly 100 microns.Entry conductor 15 is near materials chamber edge, and delivery channel 16 is near another edge in micro perfusion chamber; Entry conductor 15 and delivery channel 16 are kapillary, and internal diameter is 0.65 millimeter.Delivery channel 16 pressed close to by thermopair 17, comes to insert micro perfusion chamber completely, directly monitor the temperature of solution environmental residing for egg cell in real time in the cavity place bending be made up of short groove 132 and elongated slot 141.
Circularly cooling unit 2 is close to upper wall 11, comprises cavity 21, and it has refrigerating fluid torus 26, and two ends are respectively arranged with refrigerating fluid import 22 and refrigerating fluid outlet 23.Cavity 21 is provided with entry conductor through hole 24 and delivery channel through hole 25, is respectively used to place micro perfusion chamber entry conductor 15 and delivery channel 16.The making material of cavity 21 is dimethyl silicone polymer; Refrigerating fluid import 22 and refrigerating fluid outlet 23 are connected respectively to the entrance and exit of low temperature bath, and by the feedback temperature of thermopair 17, the liquid controlling micro perfusion chamber reaches specified temp.
Load egg cell to micro perfusion chamber from entry conductor, then inject egg cell nutrient solution to micro perfusion chamber from entry conductor; Extract egg cell nutrient solution out from delivery channel, egg cell is stuck in fixed dam place and is fixed, and due to the protection of egg cell oolemma, fixed dam can not have an impact to the form of egg cell, and solution continues flowing.Meanwhile, the application's circularly cooling unit is used for making liquid in micro perfusion chamber reach 5 DEG C, 10 DEG C and 15 DEG C respectively; The temperature of solution residing for egg cell is directly measured by the temperature sensing device being built in micro perfusion chamber.
Be under the condition of 5 DEG C in temperature, first continue to inject egg cell nutrient solution, then stop injecting, then change the egg cell nutrient solution injected containing 1mol/L ethylene glycol into.Perfusion microscope device makes egg cell at such a temperature, experiences above-mentioned change procedure and measures its volume response situation, and then determining the permeability parameter of cell membrane.Egg cell volume continually varying photo after interpolation 1mol/L ethylene glycol that measurement result provides for the embodiment of the present application see Fig. 6, Fig. 6.As shown in Figure 6, under the protection of oolemma, the form of fixed dam on egg cell does not affect, and volumetric contraction and recovery occur egg cell in oolemma.
During unloading egg cell, inject solution from delivery channel, extract solution from entry conductor, egg cell is drawn out of along with solution.
As seen from the above embodiment, microdevice described in the application can be the test of egg cell permeability experiment and provides reliable and controlled test environment, thus for studying egg cell perviousness.
Claims (10)
1., for studying a microdevice for egg cell, comprise egg cell micro perfusion unit and circularly cooling unit; Described egg cell micro perfusion unit comprises:
For the first cover plate and second cover plate of fixing micro perfusion unit; Described circularly cooling unit is close to the first cover plate;
The first film and the second film is disposed with between described first cover plate and the second cover plate; Described the first film is provided with the first cutting, described second film is provided with the second cutting, described second cutting and the first cutting form micro perfusion chamber; Described micro perfusion chamber is provided with entry conductor and delivery channel, for perfusion egg cell solution; Temperature sensing device is provided with in described micro perfusion chamber;
Between described entry conductor and delivery channel, in described micro perfusion chamber, be provided with fixed dam; Described fixed dam is used for fixing egg cell position, and allows solution to pass through.
2. microdevice according to claim 1, is characterized in that, the making material of described first cover plate and the second cover plate is independently selected from silex glass.
3. microdevice according to claim 1 and 2, is characterized in that, the making material of described the first film and the second film is silica gel.
4. microdevice according to claim 3, is characterized in that, the thickness of described the first film is 10 microns ~ 400 microns; The thickness of described second film is 5 microns ~ 140 microns;
The quantity of described fixed dam is 1; Described fixed dam is arranged on the first film, and has gap between its lower end and the second film.
5. microdevice according to claim 4, is characterized in that, the width in described micro perfusion chamber is 110 microns ~ 5 millimeters; The height that fixed dam place is removed in described micro perfusion chamber is 100 microns ~ 1 millimeter.
6. microdevice according to claim 5, is characterized in that, the making material of described fixed dam is silica gel, and itself and the first film are one-body molded.
7. microdevice according to claim 1, is characterized in that, described entry conductor and delivery channel are the kapillary that internal diameter is 0.3 millimeter ~ 1 millimeter.
8. microdevice according to claim 1, is characterized in that, described circularly cooling unit comprises:
There is the cavity of refrigerating fluid torus; Described cavity two ends are respectively arranged with refrigerating fluid import and refrigerating fluid outlet;
Described cavity is provided with entry conductor through hole and delivery channel through hole, is respectively used to place described entry conductor and delivery channel.
9. microdevice according to claim 8, is characterized in that, the making material of described cavity is dimethyl silicone polymer.
10. a microscopic system, for measuring the permeability parameter of egg cell, comprising:
Microdevice in claim 1 ~ 9 described in any one;
And the perfusion microscope device to be connected with described microdevice.
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| CN201510016969.2A CN104483253B (en) | 2015-01-13 | 2015-01-13 | A kind of microdevice and microscopic system for studying egg cell |
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