CN104480690A - Desizing composite enzyme containing fungus alpha-amylase and preparation method of desizing composite enzyme - Google Patents
Desizing composite enzyme containing fungus alpha-amylase and preparation method of desizing composite enzyme Download PDFInfo
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Abstract
The invention discloses a desizing composite enzyme containing fungus alpha-amylase and a preparation method of the desizing composite enzyme. A desizing composite enzyme for textile use, which is high in storage stability, high in enzyme activity and efficacy, comprehensive in enzyme system, excellent in desizing effect, and suitable for the modern printing and dyeing technology is prepared from heat-resistant fungus alpha-amylase as a main raw material and mould culture, a malt extract, a nonionic surfactant, lipase, an activator, a protective agent, an antioxidant and the like which are scientifically compounded. The desizing percentage, the softening property (hand feeling) and the physical and mechanical properties of desized textile can be obviously improved. Compared with a control group, the desizing composite enzyme has the advantages that two hand feeling grades are improved; the desizing percentage is improved by 4.88%; the capillary effect is improved by 48.48%; the white index is improved by 33.11%; the strength is improved by 35.82%; the desizing effect is excellent; the cottonseed hull can be effectively removed; the use amount of chemicals is reduced; and the environment is protected.
Description
Technical field
The present invention relates to weaving destarch enzyme, specifically a kind of destarch complex enzyme containing fungal alpha-amylase and preparation method thereof.
Background technology
Fabric is in weaving process, and fiber needs starching, increases fastness.Knit that base carries out dyeing, bleaches, stamp time need slurry to remove, also need the slurry of stamp to remove after stamp.The quality of destarch, directly affects the quality of finished product, as feel, whiteness, fineness, tinctorial yield, white core and intensity etc.Mostly adopt starch size starching at present, and the method for destarch is a lot, can use caustic soda, sulfuric acid, hydrogen peroxide etc., but these chemical products not only damages fabric, troublesome poeration, and pollutes the environment.Utilize enzyme preparation α-amylase under certain condition, starch slurry can be become dextrin rapidly, the soluble dextrins of post liquefaction is cleaned with washing, reaches the object of destarch.Low form α-amylase, is best suited for the discontinuous technique of banking up, and General Requirements water temperature 50 ~ 70 DEG C, pH value is about 6 ~ 7.The biology enzyme of high temperature destarch is through gene-modified heat resistant type α-amylase, serviceability temperature can reach 90 ~ 115 DEG C, in 1 ~ 3min, starch size can be stripped fast under Steaming conditions, be applicable to continuous process, greatly improve destarch efficiency, and the chemical substances (PVA also can use the destarch of PVA catabolic enzyme) such as PVA in mixed slurry can be removed simultaneously.Biology enzyme has selectivity, and amylase only has decomposition to starch, to fibrous zero damage; After process, fabric feeling ratio naoh treatment is significantly improved; Can simplification of flowsheet, reduce sewage discharge, enzyme liquid after discharge on environment without impact, the requirement of environmental protection can be met.Biology enzyme destarch is widely used, and can be used for the slurry that goes after yarn-dyed fabric, cotton, corduroy, canvas, burlap, artificial cotton, Fu Chunfang, jean, leisure wear, linen-cotton shirt, towel, knitted cotton underwear and starching stamp and processes.
Its superiority of enzyme process destarch is a lot, adopt by more and more multiplex (MUX) factory, particularly some fine fabrics enzyme desizings.Abroad, enzyme process desizing accounts for significant proportion.Be characterized in: efficient high-speed, be applicable to high temperature destarch, the time is short, and destarch rate can reach 90% one 95%; Acidity of Aikalinity is moderate, and fabric is injury-free, fabrics feel soft after destarch, plentiful, and fineness is strong, bright; Destarch and fixation with bathing process, shortened process, can be raised labour productivity; White for refining, capillary effect more than 40% can be improved, emplastic easy cleaning, environmentally safe.And can improve work working condition, save fuel, reduce costs, can continuous mass production be used for.
At present, commercially available destarch enzyme mainly contains low temperature destarch enzyme, wide temperature destarch enzyme and small part high temperature resistant destarch enzyme (resistance to 100-115 DEG C high temperature, be applied to continuous decatizing desizing), along with the demand of energy-saving and emission-reduction, low cost, wide temperature destarch enzyme, especially the destarch enzyme of low-temperature-resistant is the Main way of demand.The pre-treatment process of a Chinese patent CN 103437140 A combing polyester-cotton blend bleached cotton fabric, it comprise singe, destarch, bleaching, mercerising, heat-setting process, amylase 2 000L 2-5g is added in every premium on currency in described destarch operation, NaCl 3-4g, bleeding agent 3-4g, material groove temperature 50-55 DEG C, bank up 12-14 hour.Chinese patent CN 101880970 A discloses the enzyme auxiliary agent used in a kind of cotton linen, linen rayon gray fabric cold batching process, is prepared from by the raw material of following weight percents: destarch enzyme 3-5%; Flax degumming enzyme 10-15%; Neutral cellulase 0.5-2%; High-efficient penetrant 3-5%; Distilled water 73-83.5% and preparation method thereof, described destarch enzyme is purchased from Suzhou Bai Sheng Chemical Co., Ltd., and model is the destarch enzyme of TC-2000.But above-mentioned destarch enzyme exists mainly with single enzyme form greatly, growing starching raw material and the demand of technique can not be met, such as: 1) starching raw material is except using natural or modified starch, sometimes also with other mixed with polymers, such as PVAC polyvinylalcohol, polyacrylic acid PAA or carboxyl methyl cellulose; 2) a small amount of fat or oily substance etc. may also be added in order to lubricate warp yam surface in slurry; 3) amylase molecules amount is comparatively large simultaneously, is difficult to rapid osmotic and carries out catalysis to fabric face and nexine contact starch molecule; 4) diastatic enzyme activity plays also main dependence calcium ion and sodium ion activation, otherwise is difficult to play; 5) storage stability of destarch enzyme and vigor poor.The existence of these factors seriously restricts and affects the effect of destarch.
Therefore, prepare that a kind of storage stability is high, the weaving destarch complex enzyme of enzyme activity and effect is high, enzyme system is comprehensive, destarch is effective applicable modern dyeing and printing process is responsibility and the pursuit of those skilled in the art.
Summary of the invention
Technical problem solved by the invention is the defect overcoming existing destarch enzyme, with high temperature resistant fungal alpha-amylase for main raw material, the composite mycotic culture thing containing high temperature resistant fungal alpha-amylase and mould viable bacteria body of science, containing the malt extract enriching amylase system, there is diffusion and chemosmotic non-ionic surface active agent, there is the lipase of degraded fat effect, there is the activator promoting that enzyme activity gives full play to, there is the protective agent and antioxidant etc. that prevent complex enzyme inactivation because of change of external conditions, prepare a kind of storage stability high, enzyme activity and effect high, enzyme system is comprehensive, the weaving destarch complex enzyme of the applicable modern dyeing and printing process that destarch effect is excellent.
In order to achieve the above object, the present invention is by the following technical solutions:
Containing a destarch complex enzyme for fungal alpha-amylase, prepared by the raw material of following components by weight percent:
Fungal alpha-amylase 40-60 part, mycotic culture thing 20-30 part, malt extract 15-25 part, lipase 3-10 part, non-ionic surface active agent 2-8 part, activator 2-6 part, protective agent 1-3 part, antioxidant 0.3-0.5 part;
Described fungal alpha-amylase and mycotic culture thing are prepared through culture medium and fermentation technology optimization liquid deep layer fermenting by trichoderma reesei (Trichoderma reesei) 901-18, this bacterial strain is preserved in China typical culture collection center on November 24th, 2013, address is Wuhan City, Hubei Province Wuhan University, preserving number is CCTCC NO:M 2013602, and Classification And Nomenclature is: trichoderma reesei 901-18 Trichoderma reesei901-18;
Described trichoderma reesei 901-18 is separated by a strain to obtain through UV-LiCl-dithyl sulfate Mutation screening from the Li's Trichoderma strains of Jinshi City river levee domestic fungus cultivating base, Hunan Province soil sample, described bacterial strain feature is as follows: this bacterial strain is at PDA cultured on solid medium, the colony characteristics formed is bacterium colony is flocculence, bacterium colony is light green, bacterium colony is flat, high 0.2-0.8mm, colony edge white, neatly; Fast growth, 48h colony diameter reaches 1.5-8.5mm, and 72h reaches 30-55mm; White mycelium, has barrier film, and mycelia wall is smooth, and diameter is at 2-5 μm.Conidiophore occurs, to life on side shoot from the short lateral branch of mycelia.The optimal pH 3.0-6.5 of this bacterial strain α-amylase Producer; Optimum temperature is 27-30 DEG C;
Preferably, the preparation method of described fungal alpha-amylase comprises the following steps: the bacterial classification of intact trichoderma reesei CCTCC NO:M 2013602 is cultured to seeding tank through slant strains activation, one-level, secondary, three grades of liquid seeds expansions, by seeding tank liquid seeds with 6% inoculum concentration access fermentation tank culture medium, cultivation temperature 27-30 DEG C, mixing speed 120-180r/m, ventilation 1-3vvm, incubation time 10-15h; Then with 1-2 DEG C/h rate of temperature fall slow cooling to 10-15 DEG C, mixing speed 250-300r/m, ventilation 1-2vvm, constant temperature culture 15-20h; Continue with 1-2 DEG C/h rate of temperature fall slow cooling to 2-5 DEG C, now, seeding tank liquid seeds is added access fermentation tank with 4% inoculum concentration, constant temperature culture 20-30h; Finally slowly be warming up to 10-15 DEG C, mixing speed 200-400r/m, ventilation 1-2vvm, constant temperature culture 15-20h with 1-2 DEG C/h heating rate; Continue slowly to be warming up to 27-30 DEG C, constant temperature culture 15-20h with 1-2 DEG C/h heating rate; Fermentation liquor is filtered, concentrated, allotment, essence filter, dry solid fungal alpha-amylase;
In described fungal alpha-amylase preparation process, fermentation broth enzyme vigor is up to 12000-15000u/ml; Enzyme Acclimation temperature wider range, between 50-75 DEG C, optimum temperature, at 65 DEG C, is preserved 3h and is still had more than 80% enzyme work, have good heat endurance and preserve active at 70 DEG C; This enzyme optimal reaction pH value is 5.5, and living at the glucose-6-phosphate dehydrogenase of pH value 4.5-7.0 remains on more than 70%, higher than existing fungal alpha-amylase enzyme activity, enzyme effect optimum pH wide scope, and resistance to temperature is high;
Described slant medium consists of: glucose 20g, agar 20g, Chinese herbal medicine extract 5-10g, yeast extract 4g, dipotassium hydrogen phosphate 2g, magnesium chloride 0.6g, potassium chloride 0.8g, distilled water l000mL, pH value 5.8,121 DEG C of sterilizing 20min.
Described one-level, secondary, three grades of seed culture mediums consist of: wheat bran 60-80g, corn flour 50-60g, beancake powder 35-40g, trehalose 10-15g, fish meal 6-10g, ammonium chloride 10-12g, calcium chloride 5-10g, Chinese herbal medicine extract 5-10g, magnesium sulfate 2-4g, dipotassium hydrogen phosphate 1-3g, pure water l000mL, pH value 5-7,121 DEG C of sterilizing 20min;
Described seed tank culture base consists of: wheat bran 60-80g, corn flour 50-60g, beancake powder 35-40g, trehalose 10-15g, Chinese herbal medicine extract 10-15g, fish meal 6-10g, ammonium chloride 10-12g, calcium chloride 5-10g, magnesium sulfate 2-4g, dipotassium hydrogen phosphate 1-3g, pure water l000mL, pH value 5-7,121 DEG C of sterilizing 20min;
Described seeding tank zymotic fluid cell concentration is 7.0x 10
8-8.0x 10
8individual/ml;
Described fermentation tank culture medium consists of: wheat bran 60-80g, corn flour 50-60g, malt extract 40-60g, beancake powder 35-40g, Chinese herbal medicine extract 20-30g, trehalose 10-30g, fish meal 6-10g, ammonium chloride 10-12g, calcium chloride 5-10g, magnesium sulfate 2-4g, dipotassium hydrogen phosphate 1-3g, potassium nitrate 1-2g, zinc sulfate 0.1-0.2g, pure water l000mL, pH value 5-7,121 DEG C of sterilizing 20min;
The preparation method of described Chinese herbal medicine extract is as follows:
Count by weight, take Radix Astragali 50-60 part, Radix Angelicae Sinensis 40-50 part, Radix Codonopsis 35-45 part, Radix Glycyrrhizae 35-45 part, cordate houttuynia 25-35 part, Divine Comedy 20-30 part, radix bupleuri 10-15 part, root of large-flowered skullcap 10-15 part; Said herbal medicine being crushed to particle diameter is less than 2 millimeters, then Homogeneous phase mixing add the water of 3-6 times of weight in container, control temperature 70 DEG C-90 DEG C keeps 2-4h, then 45-60 DEG C is cooled to, the mixing enzyme preparation adding mixed material gross weight 5-10% carries out enzymolysis, be 5.5-6.8 by lactic acid adjust ph, enzymolysis 2-4h, finally add the mixture of mixed material 0.5-3 times of w ethanol and propyl alcohol, the mass ratio of ethanol and propyl alcohol mixing is 1:1.5, control temperature to 60 DEG C-78 DEG C of maintenance 3-4h, filter, obtain the first filtrate; Add the water of filter residue 1-3 times of weight, control temperature 85 DEG C-95 DEG C keeps 1-3h, is then cooled to 25-35 DEG C, filters, obtains the second filtrate; First filtrate and the second filtrate are merged according to mass ratio 2-4:1-3, filter vacuum concentrates postlyophilization, pulverizes and obtains Chinese herbal medicine extract;
Described mixed enzyme is dextranase, zytase, pentosanase, pectase 4:4:2:1 Homogeneous phase mixing in mass ratio.
Described mycotic culture thing has stronger microbial activity and anti-heat stress, high vigor can be produced on the one hand by fermentation in desizing processes, the fungal alpha-amylase that thermostability is strong, most importantly mycotic spore can be made even by the dynamic response of fermentation, penetrate into surface and the fabric fibre cytoplasm of fabric fully, abundant decomposition starch wherein, also can promote that other complex enzyme component permeates simultaneously, what give full play to enzyme component should have effect, effectively to improve fabrics feel soft after destarch rate and capillary effect and destarch, plentiful, fineness and dyeing, fixation intensity,
Preferably, fermentation liquor reduced pressure concentration when prepared by described mycotic culture thing above-mentioned fungal alpha-amylase, freeze drying, low-temperature grinding obtain;
Described mycotic culture thing viable bacteria content is 7x10
10-9x10
10cFU/g.
Containing abundant vegetable diastase in described malt extract, especially α-amylasecontent is the highest, also has a small amount of various plants enzyme such as protease, hemicellulase, esterase, oxidoreducing enzyme, gentle enzymolysis can be carried out to fabric, can not cause any harm to fabric fibre, improve the pliability after fabric desizing rate and destarch further;
Also containing the nutriment such as plant polyose and monose in described malt extract, not containing plant amylum, (phytoenzyme that the macromolecular substances such as plant amylum, vegetable protein has been dissolved enzymolysis in leaching process is the Small molecular such as low-molecular polysaccharide and monose hydrolysate to vegetable protein.), not only can be that destarch complex enzyme provides comprehensively, abundant phytoenzyme, also can be used as the composition of fermentation medium time prepared by fungal alpha-amylase and mycotic culture thing, improve fungal alpha-amylase vigor and microorganism enzymatic productivity; Act synergistically with non-ionic surface active agent simultaneously, advantageously in fungal alpha-amylase and the slow enzymolysis of lipase and rapid osmotic, gentle, appropriate destarch;
Described malt extract can be used as the carrier of destarch complex enzyme;
Described malt extract adopts the low temperature extractive technique such as the ultrasonic extraction of microwave radiation technology and high-pressure pulse electric extraction, ultrafiltration concentration, effectively improves raw material availability, phytoenzyme activity and productive rate;
The preparation method of described malt extract is: by fructus hordei germinatus and wheat malt 6-8:3-5 Homogeneous phase mixing in mass ratio, be crushed to granularity 0.5-1mm, obtain pulverizing Fructus Hordei Germinatus; Add and pulverize malt quality 1-3 water doubly, be 3-4 by lactic acid adjust ph, Microwave Extraction is carried out under power 150-300W, frequency 2000Hz condition, wherein, each microwave irradiation total time 60-80s, carry out compartment irradiation: irradiation 10s, interval 10s, control temperature 20-35 DEG C, irradiation like this 10 times, under power 200-300W, frequency 30-40KHz condition, carry out ultrasonic assistant extraction simultaneously; Insulation 1-3h, then, Microwave Extraction is carried out under power 200-400W, frequency 2000Hz condition, wherein, each microwave irradiation total time 90-105s, carry out compartment irradiation: irradiation 15s, interval 10s, control temperature 40-60 DEG C, irradiation like this 10 times, under power 300-500W, frequency 40-50KHz condition, carry out ultrasonic assistant extraction simultaneously; 55-65 DEG C of insulation 30-60min; Last Temperature fall is to room temperature, and in electric-field intensity 25-35kV/cm, burst length 400-600 μ s, carries out high-pressure pulse electric (PEF) and extract 15-20min under pulse frequency 200-300Hz condition; Extracting liquid filtering obtains the first filtrate, adds the water rinse of filter residue 2 times, filters to obtain the second filtrate, and by the first filtrate and the second filtrate 1:1-2 Homogeneous phase mixing in mass ratio, namely mixed liquor ultrafiltration concentration, freeze drying, low-temperature grinding obtain malt extract.
Described non-ionic surface active agent can help infiltration, degreasing and scale removal in desizing processes, promote and extend fungal alpha-amylase, mycotic spore, the contacting and the reaction time of lipase and fabric different parts substrate specificity (amylum body, fat granule cell), fully, effectively to degrade fat, help fungal alpha-amylase evenly to spread, permeate, improve flexibility and the destarch uniformity of fabric;
Preferably, described non-ionic surface active agent is environment-friendly type non-ionic surface active agent, easy biodegradation, and noresidue is without any side effects to human body, biology and environment;
More preferably, the quality component of described non-ionic surface active agent is: alkyl polyglycoside (APG) 25-35 part, methyl glucamine (AGA) 15-25 part, N-dodecyl ethylenediamine triacetic acid sodium (ED3A) 8-12 part, isomery alcohol ether carboxylate AEC-1107 8-12 part, Lauric Acid Monoethanolamide Ether Carboxylate (LAEC) 5-8 part, peregal c-125 3-5 part, JFC 3-5 part; Above-mentioned raw materials is commercial solid, powdery commodity.
Described activator is formed by the inorganic salts Homogeneous phase mixing of following quality component: calcium chloride 60-80 part, sodium chloride 30-50 part, zinc chloride 10-15 part, magnesium chloride 5-10 part.
Described protective agent is made up of the raw material of following parts by weight: GL-B 15-25 part, trehalose 15-25 part, (NH
4)
2sO
46-10 part, Cys2-4 parts.
Described antioxidant is any one or a few combination in grape pip procyanidin, Rosmarinus officinalis extract and apricot leaf extract;
Preferably, described antioxidant is grape pip procyanidin, Rosmarinus officinalis extract and apricot leaf extract 5-7:2-4:1-4 Homogeneous phase mixing in mass ratio;
Described grape pip procyanidin, Rosmarinus officinalis extract and apricot leaf extract are commercial commodity.
The preparation method of above-mentioned destarch complex enzyme, comprises the steps:
First by described protective agent, activator ultramicro grinding respectively; Homogeneous phase mixing; immediately add malt extract, mycotic culture thing Homogeneous phase mixing 20-30min successively; then fungal alpha-amylase, lipase, non-ionic surface active agent Homogeneous phase mixing is added successively; finally add antioxidant, after mixing, pack and obtain destarch complex enzyme.
Another object of the present invention is the application of described destarch complex enzyme in weaving desizing.
Using method:
1. complex enzyme action condition: adapt to pH value 4.5-7.0, optimum pH 5.5; Adaptive temperature 50-75 DEG C, optimal reactive temperature 60 DEG C.
2. using method: 1) during process, fabric open width after singing pads destarch complex enzyme liquid, bath raio 1:8-18 (with fabric over dry restatement after singing); 2) by complex enzyme and 60 DEG C of water 1:10 Homogeneous phase mixing in mass ratio, adjusted to ph is 5.5, and activation 30-50min, result of use is best.
3. use amount: often liter of complex enzyme liquid adds complex enzyme 1-2g.
Beneficial effect:
The present invention is containing the destarch complex enzyme of fungal alpha-amylase, with high temperature resistant fungal alpha-amylase for main raw material, the composite mycotic culture thing containing high temperature resistant fungal alpha-amylase and mould viable bacteria body of science, containing the malt extract enriching amylase system, there is diffusion and chemosmotic non-ionic surface active agent, there is the lipase of degraded fat effect, there is the activator promoting that enzyme activity gives full play to, there is the protective agent and antioxidant etc. that prevent complex enzyme inactivation because of change of external conditions, prepare a kind of storage stability high, enzyme activity and effect high, enzyme system is comprehensive, the weaving destarch complex enzyme of the applicable modern dyeing and printing process that destarch effect is excellent, the destarch rate of fabric after destarch can be significantly improved, softness (feel) and physical and mechanical properties, compared with control group, feel rank improves 2 grades, destarch rate improves 4.88%, capillary effect improves 48.48%, whitness index improves 33.11%: powerful raising 35.82%, destarch effect is excellent, effectively can remove cotton seed hulls, decreases the use amount of chemical article, protects environment.
Be in particular in:
1. fungal alpha-amylase of the present invention and mycotic culture thing are prepared through culture medium and fermentation technology optimization liquid deep layer fermenting by trichoderma reesei (Trichoderma reesei) 901-18, deposit number is CCTCC NO:M 2013602, and fungal alpha-amylase fermentation broth enzyme vigor is up to 12000-15000u/ml; Enzyme Acclimation temperature wider range, between 50-75 DEG C, optimum temperature, at 65 DEG C, is preserved 3h and is still had more than 80% enzyme work, have good heat endurance and preserve active at 70 DEG C; This enzyme optimal reaction pH value is 5.5, and living at the glucose-6-phosphate dehydrogenase of pH value 4.5-7.0 remains on more than 70%, higher than existing fungal alpha-amylase enzyme activity, enzyme effect optimum pH wide scope, and resistance to temperature is high, is more applicable to weaving desizing.
2. mycotic culture thing of the present invention has stronger microbial activity and anti-heat stress, by fermentation in desizing processes, high vigor can be produced on the one hand, the fungal alpha-amylase that thermostability is strong, most importantly mycotic spore can be made even by the dynamic response of fermentation, penetrate into surface and the fabric fibre cytoplasm of fabric fully, abundant decomposition starch wherein, also can promote that other complex enzyme component permeates simultaneously, what give full play to enzyme component should have effect, effectively to improve fabrics feel soft after destarch rate and capillary effect and destarch, plentiful, fineness and dyeing, fixation intensity.
3. contain abundant vegetable diastase in malt extract of the present invention, especially α-amylasecontent is the highest, also has a small amount of various plants enzyme such as protease, hemicellulase, esterase, oxidoreducing enzyme, gentle enzymolysis can be carried out to fabric, can not cause any harm to fabric fibre, improve the pliability after fabric desizing rate and destarch further; Also contain the nutriment such as plant polyose and monose in malt extract, containing plant amylum, (phytoenzyme that the macromolecular substances such as plant amylum, vegetable protein has been dissolved enzymolysis in leaching process is the Small molecular such as low-molecular polysaccharide and monose hydrolysate to vegetable protein simultaneously.), not only can be the phytoenzyme that destarch complex enzyme provides comprehensively, enriches, also can be used as the fermentation medium components of the mycotic spore in mycotic culture thing, improve fungal alpha-amylase vigor and microorganism enzymatic productivity; Act synergistically with non-ionic surface active agent simultaneously, advantageously in fungal alpha-amylase and the slow enzymolysis of lipase and rapid osmotic, gentle, appropriate destarch.Described malt extract adopts the low temperature extractive technique such as the ultrasonic extraction of microwave radiation technology and high-pressure pulse electric extraction, ultrafiltration concentration, effectively improves raw material availability, phytoenzyme activity and productive rate.
4. the composite environment-friendly type non-ionic surface active agent of the main science of non-ionic surface active agent of the present invention, easy biodegradation, noresidue, without any side effects to human body, biology and environment; Infiltration, degreasing and scale removal can be helped in desizing processes, promote and extend fungal alpha-amylase, mycotic spore, the contacting and the reaction time of lipase and fabric different parts substrate specificity (amylum body, fat granule cell), fully, effectively to degrade fat, help fungal alpha-amylase evenly to spread, permeate, improve flexibility and the destarch uniformity of fabric.
5. the science of destarch complex enzyme antioxidant of the present invention is composite, complex enzyme enzyme molecular structure effectively can be prevented to be oxidized and to cause loss of enzyme activity, improve enzyme activity stability.12 months are stored under 0 DEG C and 40 DEG C of conditions, in complex enzyme, the loss alive of single enzyme enzyme is respectively 0.40% and 0.53%, 20.0% and 66.9% is reduced respectively than comparative example, effectively to prevent from links such as packaging, storage, transport, uses, because environment change, method of operating are improper and cause the inactivation of enzyme, especially can preventing the enzyme deactivation that high temperature causes.
6. in destarch complex enzyme of the present invention, protective agent science is composite, effectively slow down the moisture regain of complex enzyme formulation; Can strengthen complex enzyme simultaneously resistance toly to freeze, heat resistance, keep identical enzyme activity, its heat resisting temperature can improve 20-30 DEG C, resistance to cryogenic temperature can reduce 10-15 degree Celsius, effectively prevent the loss of complex enzyme enzyme activity in transport, preservation and use procedure, extend the shelf-life of complex enzyme, reach same enzyme activity, can 2-3 be extended than the like product shelf-life.
7. destarch complex enzyme of the present invention adds inorganic salts as activator, especially science is composite, and α-amylase enzyme activity gives full play to necessary calcium ion, create the optimum condition of enzyme catalysis, given full play to the vigor of phytoenzyme and microbial enzyme, improve complex enzyme action potency.
Detailed description of the invention
The present invention is described below by specific embodiment.Unless stated otherwise, technological means used in the present invention is method known in those skilled in the art.In addition, embodiment is interpreted as illustrative, but not limits the scope of the invention, and the spirit and scope of the invention only limited by claims.To those skilled in the art, under the prerequisite not deviating from essence of the present invention and scope, the various change carry out the material component in these embodiments and consumption or change also belong to protection scope of the present invention.
Prepared by embodiment 1 raw material
1. the preparation of fungal alpha-amylase:
The preparation method of described fungal alpha-amylase comprises the steps:
(1) actication of culture
The slant strains of intact trichoderma reesei CCTCC NO:M 2013602 is inoculated in slant medium, cultivates 40h for 30 DEG C and carry out actication of culture, so activation 3 times;
(2) liquid seeds expands cultivation
1. first order seed is cultivated: by step (1) activation back bevel bacterial classification with spore under aseptic washing, access in 500 ml shake flasks, liquid seed culture medium loading amount 100 milliliters, 30 DEG C, 100rpm shaking table cultivation 40h;
2. secondary seed cultivate: by first order seed according to 10% inoculum concentration access in 500 milliliters of secondary seed shaking flasks, condition of culture is identical with first order seed;
3. three grades of seed culture: secondary seed is accessed in 5000 milliliters of three grades of seed flask with 8% inoculum concentration, fluid nutrient medium loading amount 1000 milliliters, 30 DEG C, 100rpm shaking table cultivation 40h;
4. seed tank culture: the first class seed pot being 150L with 8% inoculum concentration access total measurement (volume) by three grades of seeds, seed tank culture base loading amount 100L, control ph is 6, cultivation temperature 29 DEG C, mixing speed 300rpm, ventilation (V/V) 1:1, incubation time 40h;
Described seeding tank zymotic fluid cell concentration is 8.0x 10
8individual/ml;
(3) ferment tank
By seeding tank liquid seeds in step (2) with 6% inoculum concentration access fermentation tank culture medium, cultivation temperature 28 DEG C, mixing speed 150r/m, ventilation 2vvm, incubation time 12h; Then with 2 DEG C/h rate of temperature fall slow cooling to 12 DEG C, mixing speed 280r/m, ventilation 1.5vvm, constant temperature culture 18h; Continue with 2 DEG C/h rate of temperature fall slow cooling to 4 DEG C, now, first class seed pot zymotic fluid is added access fermentation tank with 4% inoculum concentration, constant temperature culture 25h; Finally slowly be warming up to 12 DEG C, mixing speed 300r/m, ventilation 1.5vvm, constant temperature culture 18h with 2 DEG C/h heating rate; Continue slowly to be warming up to 28 DEG C, constant temperature culture 18h with 2 DEG C/h heating rate;
(4) fermentation liquor filter, concentrated, allotment, essence filter, dry solid fungal alpha-amylase.
After the fungal alpha-amylase prepared through said method at room temperature preserves 12 months, enzyme loss of living is 1.6%, and zymotic fluid α-amylase enzyme activity can reach 15000U/mL.
Described slant medium consists of: glucose 20g, agar 20g, Chinese herbal medicine extract 7g, yeast extract 4g, dipotassium hydrogen phosphate 2g, magnesium chloride 0.6g, potassium chloride 0.8g, distilled water l000mL, pH value 5.8,121 DEG C of sterilizing 20min.
Described one-level, secondary, three grades of seed culture mediums consist of: wheat bran 70g, corn flour 55g, beancake powder 38g, trehalose 12g, fish meal 8g, ammonium chloride 11g, calcium chloride 8g, Chinese herbal medicine extract 8g, magnesium sulfate 3g, dipotassium hydrogen phosphate 2g, pure water l000mL, pH value 6,121 DEG C of sterilizing 20min;
Described seed tank culture base consists of: wheat bran 70g, corn flour 55g, beancake powder 38g, trehalose 12g, Chinese herbal medicine extract 12g, fish meal 8g, ammonium chloride 11g, calcium chloride 8g, magnesium sulfate 3g, dipotassium hydrogen phosphate 2g, pure water l000mL, pH value 6,121 DEG C of sterilizing 20min;
Described fermentation tank culture medium consists of: wheat bran 70g, corn flour 55g, malt extract 50g, beancake powder 38g, Chinese herbal medicine extract 25g, trehalose 20g, fish meal 8g, ammonium chloride 11g, calcium chloride 8g, magnesium sulfate 3g, dipotassium hydrogen phosphate 2g, potassium nitrate 2g, zinc sulfate 0.2g, pure water l000mL, pH value 6,121 DEG C of sterilizing 20min;
The preparation method of described Chinese herbal medicine extract is as follows: count by weight, takes the Radix Astragali 55 parts, Radix Angelicae Sinensis 45 parts, Radix Codonopsis 40 parts, 40 parts, Radix Glycyrrhizae, cordate houttuynia 30 parts, Divine Comedy 25 parts, radix bupleuri 12 parts, the root of large-flowered skullcap 12 parts; Said herbal medicine being crushed to particle diameter is less than 2 millimeters, then Homogeneous phase mixing add the water of 5 times of weight in container, control temperature 80 DEG C keeps 3h, is then cooled to 52 DEG C, the mixing enzyme preparation adding mixed material gross weight 8% carries out enzymolysis, be 6.2 by lactic acid adjust ph, enzymolysis 3h, finally adds the mixture of mixed material 2 times of w ethanol and propyl alcohol, the mass ratio of ethanol and propyl alcohol mixing is 1:1.5, control temperature to 70 DEG C keeps 4h, filters, obtains the first filtrate; Add the water of filter residue 2 times of weight, control temperature 90 DEG C keeps 2h, is then cooled to 30 DEG C, filters, obtains the second filtrate; First filtrate and the second filtrate are merged according to mass ratio 2.5:1.5, filter vacuum concentrates postlyophilization, pulverizes and obtains Chinese herbal medicine extract;
Described mixed enzyme is dextranase, zytase, pentosanase, pectase 4:4:2:1 Homogeneous phase mixing in mass ratio.
2. the preparation of mycotic culture thing: fermentation liquor reduced pressure concentration time prepared by above-mentioned fungal alpha-amylase, freeze drying, low-temperature grinding obtain mycotic culture thing; In described mycotic culture thing, viable bacteria content is 9x10
10cFU/g.
3. the preparation of malt extract:
The preparation method of described malt extract is: by fructus hordei germinatus and wheat malt 7:4 Homogeneous phase mixing in mass ratio, be crushed to granularity 0.8mm, obtain pulverizing Fructus Hordei Germinatus; Add the water pulverizing malt quality 2 times, be 3.5 by lactic acid adjust ph, Microwave Extraction is carried out under power 200W, frequency 2000Hz condition, wherein, each microwave irradiation total time 70s, carry out compartment irradiation: irradiation 10s, interval 10s, control temperature 30 DEG C, irradiation like this 10 times, under power 250W, frequency 35KHz condition, carry out ultrasonic assistant extraction simultaneously; Insulation 2h, then, Microwave Extraction is carried out under power 300W, frequency 2000Hz condition, wherein, each microwave irradiation total time 105s, carry out compartment irradiation: irradiation 15s, interval 10s, control temperature 50 DEG C, irradiation like this 10 times, under power 400W, frequency 45KHz condition, carry out ultrasonic assistant extraction simultaneously; 60 DEG C of insulation 45min; Last Temperature fall is to room temperature, and in electric-field intensity 30kV/cm, burst length 500 μ s, carries out high-pressure pulse electric (PEF) and extract 18min under pulse frequency 250Hz condition; Extracting liquid filtering obtains the first filtrate, adds the water rinse of filter residue 2 times, filters to obtain the second filtrate, and by the first filtrate and the second filtrate 1:1.5 Homogeneous phase mixing in mass ratio, namely mixed liquor ultrafiltration concentration, freeze drying, low-temperature grinding obtain malt extract.
Embodiment 2
Containing a destarch complex enzyme for fungal alpha-amylase, prepared by the raw material of following components by weight percent:
Fungal alpha-amylase 50 parts, mycotic culture thing 25 parts, malt extract 20 parts, 6 parts, lipase, non-ionic surface active agent 5 parts, activator 4 parts, protective agent 2 parts, antioxidant 0.4 part;
The quality component of described non-ionic surface active agent is: alkyl polyglycoside (APG) 30 parts, methyl glucamine (AGA) 20 parts, N-dodecyl ethylenediamine triacetic acid sodium (ED3A) 10 parts, isomery alcohol ether carboxylate AEC-1107 10 parts, Lauric Acid Monoethanolamide Ether Carboxylate (LAEC) 6 parts, peregal c-125 4 parts, JFC 4 parts;
Described activator is formed by the inorganic salts Homogeneous phase mixing of following quality component: 70 parts, calcium chloride, 40 parts, sodium chloride, zinc chloride 12 parts, 8 parts, magnesium chloride;
Described protective agent is made up of the raw material of following parts by weight: GL-B 20 parts, trehalose 20 parts, (NH
4)
2sO
48 parts, cysteine 3 parts;
Described antioxidant is grape pip procyanidin, Rosmarinus officinalis extract and apricot leaf extract 6:3:2 Homogeneous phase mixing in mass ratio;
Described fungal alpha-amylase, mycotic culture thing and malt extract are embodiment 1 to be prepared;
The preparation method of described destarch complex enzyme, comprises the steps:
First by described protective agent, activator ultramicro grinding respectively; Homogeneous phase mixing; immediately add malt extract, mycotic culture thing Homogeneous phase mixing 25min successively; then fungal alpha-amylase, lipase, non-ionic surface active agent Homogeneous phase mixing is added successively; finally add antioxidant, after mixing, pack and obtain destarch complex enzyme.
Embodiment 3
Containing a destarch complex enzyme for fungal alpha-amylase, prepared by the raw material of following components by weight percent:
Fungal alpha-amylase 40 parts, mycotic culture thing 20 parts, malt extract 15 parts, 3 parts, lipase, non-ionic surface active agent 2 parts, activator 2 parts, protective agent 1 part, antioxidant 0.3 part;
The quality component of described non-ionic surface active agent is: alkyl polyglycoside (APG) 25 parts, methyl glucamine (AGA) 15 parts, N-dodecyl ethylenediamine triacetic acid sodium (ED3A) 8 parts, isomery alcohol ether carboxylate AEC-1107 8 parts, Lauric Acid Monoethanolamide Ether Carboxylate (LAEC) 5 parts, peregal c-125 3 parts, JFC 3 parts;
Described activator is formed by the inorganic salts Homogeneous phase mixing of following quality component: 60 parts, calcium chloride, 30 parts, sodium chloride, zinc chloride 10 parts, 5 parts, magnesium chloride;
Described protective agent is made up of the raw material of following parts by weight: GL-B 15 parts, trehalose 15 parts, (NH
4)
2sO
46 parts, Cys2 part;
Described antioxidant is grape pip procyanidin, Rosmarinus officinalis extract and apricot leaf extract 5:2:1 Homogeneous phase mixing in mass ratio;
Described fungal alpha-amylase, mycotic culture thing and malt extract are embodiment 1 to be prepared;
The preparation method of described destarch complex enzyme is with embodiment 2.
Embodiment 4
Containing a destarch complex enzyme for fungal alpha-amylase, prepared by the raw material of following components by weight percent:
Fungal alpha-amylase 60 parts, mycotic culture thing 30 parts, malt extract 25 parts, 10 parts, lipase, non-ionic surface active agent 8 parts, activator 6 parts, protective agent 3 parts, antioxidant 0.5 part;
The quality component of described non-ionic surface active agent is: alkyl polyglycoside (APG) 35 parts, methyl glucamine (AGA) 25 parts, N-dodecyl ethylenediamine triacetic acid sodium (ED3A) 12 parts, isomery alcohol ether carboxylate AEC-1107 12 parts, Lauric Acid Monoethanolamide Ether Carboxylate (LAEC) 8 parts, peregal c-125 5 parts, JFC 5 parts;
Described activator is formed by the inorganic salts Homogeneous phase mixing of following quality component: 80 parts, calcium chloride, 50 parts, sodium chloride, zinc chloride 15 parts, 10 parts, magnesium chloride;
Described protective agent is made up of the raw material of following parts by weight: GL-B 25 parts, trehalose 25 parts, (NH
4)
2sO
410 parts, cysteine 4 parts;
Described antioxidant is grape pip procyanidin, Rosmarinus officinalis extract and apricot leaf extract 7:4:4 Homogeneous phase mixing in mass ratio;
Described fungal alpha-amylase, mycotic culture thing and malt extract are embodiment 1 to be prepared;
The preparation method of described destarch complex enzyme, with embodiment 2.
Embodiment 5
Containing a destarch complex enzyme for fungal alpha-amylase, prepared by the raw material of following components by weight percent:
Fungal alpha-amylase 60 parts, mycotic culture thing 20 parts, malt extract 25 parts, 10 parts, lipase, non-ionic surface active agent 8 parts, activator 2 parts, protective agent 3 parts, grape pip procyanidin 0.3 part;
The quality component of described non-ionic surface active agent is: alkyl polyglycoside (APG) 35 parts, methyl glucamine (AGA) 15 parts, N-dodecyl ethylenediamine triacetic acid sodium (ED3A) 12 parts, isomery alcohol ether carboxylate AEC-1107 12 parts, Lauric Acid Monoethanolamide Ether Carboxylate (LAEC) 5 parts, peregal c-125 3 parts, JFC 3 parts;
Described activator is formed by the inorganic salts Homogeneous phase mixing of following quality component: 80 parts, calcium chloride, 30 parts, sodium chloride, zinc chloride 10 parts, 10 parts, magnesium chloride;
Described protective agent is made up of the raw material of following parts by weight: GL-B 15 parts, trehalose 25 parts, (NH
4)
2sO
410 parts, Cys2 part;
Described fungal alpha-amylase, mycotic culture thing and malt extract are embodiment 1 to be prepared;
The preparation method of described destarch complex enzyme is with embodiment 2.
The mensuration of embodiment 6 fungal alpha-amylase zymologic property
(1) impact of temperature on enzymatic activity and the heat endurance of enzyme
Embodiment 1 fungal alpha-amylase fermentation crude enzyme liquid is measured this amylase enzyme activity respectively at 40-80 DEG C, and result shows that this enzyme has the enzymatic activity of more than 82% between 50-75 DEG C, and Acclimation temperature wider range of this enzyme is described.And thermally-stabilised experiment shows, this enzyme, after 70 DEG C of insulation 3h, in 60 DEG C, still has more than 80% enzyme and lives under pH value 5.5 condition, temperature is higher than 75 DEG C, and enzyme lives loss seriously.
(2) pH is on the impact of enzymatic activity
Embodiment 1 fungal alpha-amylase fermentation crude enzyme liquid is surveyed this enzyme enzyme respectively under pH value 3-8.5 live, result shows that this enzyme enzymatic activity when pH value 4.5-7 remains on more than 70%, and reaches maximum during pH value 5.5.
Embodiment 7 antioxidant is on the impact of destarch complex enzyme enzyme activity
Adopt the destarch complex enzyme containing fungal alpha-amylase prepared by the embodiment of the present invention 2, other raw material, material component, preparation method are identical, unique difference is that raw material components is not containing antioxidant, form comparative example, 12 months are stored respectively under 0 DEG C and 40 DEG C of conditions, detection method in " GB/T 23535-2009 lipase preparation " is adopted to measure the enzyme activity of lipase, calculate enzyme loss late alive, enzyme loss late alive refers to that the enzyme activity of actual detection and the difference of product annotation enzyme activity account for the percentage marking enzyme activity, and result is as table 1
Lipase activity power loss late in table 1 storage life complex enzyme
Above result shows, 12 months are stored under 0 DEG C and 40 DEG C of conditions, lipase in embodiment 2 reduces by 20% and 66.9% respectively than the lipase activity loss in comparative example, illustrate that the science of antioxidant is composite, significantly improve the vigor of each component enzymes in complex enzyme, enzyme is lived in losing and is significantly reduced, and effect is more remarkable especially under the high temperature conditions.
Embodiment 8 destarch complex enzyme of the present invention is used for the test of all-cotton fabric infusion process desizing
One, test site: Jinshi City woollen mill of Hunan Province pretreatment workshop.
Two, test period: on March 14 ,-2014 years on the 12nd January in 2014, last 60 days.
Three, plan design: 1. test as single-factor designs, test group and control group are set, the raw material of control group and test group, technique, equipment, operating personnel, environment and way to manage are all identical, difference is: the desizing processes of test group after singing adds destarch complex enzyme prepared by the embodiment of the present invention 2, and control group adds commercially available destarch specific enzyme.2. test group and control group are all by fabric over dry reprovision enzyme liquid after singing, bath raio 1:10, and often liter of enzyme liquid adds enzyme 1.5g.3. test group and control group respectively process 10 batches, calculating mean value.4. pair test group and control group all-cotton fabric after treatment correlated quality index detect: result is as table 2
4.1 capillary effects: semi-products are cut into 5cm × 35cm cloth along warp-wise, and one end is fixed, one end is vertically immersed in distilled water, measures the height that in 30min, water rises along warp-wise;
4.2 cotton seed hullss: range estimation is compared;
4.3 whiteness: adopt datacolourSF600 colour photometer to measure the whitness index of fabric;
4.4 destarch effects: iodo-drop method
4.4.1 the fabric sample after destarch drips several iodine liquid (0.005N), and destarch effect is divided into 4 grades by the colour generation according to test solution: excellent-brown color (primary colors), good-inner yellow outer partially blue, in-blue, poor-purplish blue or navy blue;
4.4.2 the compound method of test iodine liquid: get 18 grams of KIs and 13 grams of iodine are dissolved in water, makes total amount be 1 liter, then gets this storage liquid 5ml and be diluted to 100ml and be test fluid; Storage liquid and test fluid all will leave in dark bottles, each use before need on the former base of the fabric of starch starching drop to check its usefulness;
4.5 ultimate strengths: fabric is cut into 5cm × 35cm cloth along warp-wise or broadwise, adopt YG2026 type imported electronic fabric strength tester to measure;
4.6 feel tests: form a group by 5-10 people, touch fabric after arrangement, grading.Adopt subjective evaluation method, namely adopt the method for " pinch, touch, grab, see ", smooth, soft glutinous, plentiful, the elasticity of evaluation fabric, well-pressed, body bone and the hand feel characteristic such as active, in order to compare more intuitively, adopt 5 grades of representations, 1 grade the poorest, and 5 grades best;
Table 2 destarch complex enzyme is on the impact of all-cotton fabric quality
| Enzyme addition | Often liter of enzyme liquid adds enzyme 1.5g |
| Bath raio | 1:10 |
| Temperature | 60℃ |
| PH value | 5.5 |
| Time | 40min |
[0140]
| Test item | Test group | Control group | Difference |
| Feel (level) | 5 | 3 | 2 |
| Destarch rate (%) | 96.8 | 92.3 | 4.5(4.88％) |
| Capillary effect (cm/30min) | 19.6 | 13.2 | 6.4(48.48％) |
| Whitness index | 20.1 | 15.1 | 5(33.11％) |
| Powerful (N) | 455.8 | 335.6 | 120.2(35.82％) |
| Destarch effect | Excellent | Good | |
| Remove cotton seed hulls | Good | Generally |
Above result shows: adopt destarch complex enzyme of the present invention can significantly improve the destarch rate of fabric after destarch, softness (feel) and physical and mechanical properties, compared with control group, feel rank improves 2 grades; Destarch rate improves 4.88%; Capillary effect improves 48.48%; Whitness index improves 33.11%: powerful raising 35.82%; Destarch effect is excellent, effectively can remove cotton seed hulls.
Claims (10)
1. the destarch complex enzyme containing fungal alpha-amylase, prepared by the raw material of following components by weight percent: fungal alpha-amylase 40-60 part, mycotic culture thing 20-30 part, malt extract 15-25 part, lipase 3-10 part, non-ionic surface active agent 2-8 part, activator 2-6 part, protective agent 1-3 part, antioxidant 0.3-0.5 part;
Described fungal alpha-amylase and mycotic culture thing are prepared through liquid deep layer fermenting by trichoderma reesei (Trichoderma reesei) CCTCC NO:M2013602;
Described activator is formed by the inorganic salts Homogeneous phase mixing of following quality component: calcium chloride 60-80 part, sodium chloride 30-50 part, zinc chloride 10-15 part, magnesium chloride 5-10 part;
Described protective agent is made up of the raw material of following parts by weight: GL-B 15-25 part, trehalose 15-25 part, (NH
4)
2sO
46-10 part, Cys2-4 parts.
2. the destarch complex enzyme containing fungal alpha-amylase as claimed in claim 1, it is characterized in that, the preparation method of described fungal alpha-amylase comprises the following steps: the bacterial classification of intact trichoderma reesei CCTCC NO:M 2013602 is cultured to seeding tank through slant strains activation, one-level, secondary, three grades of liquid seeds expansions, by seeding tank liquid seeds with 6% inoculum concentration access fermentation tank culture medium, cultivation temperature 27-30 DEG C, mixing speed 120-180r/m, ventilation 1-3vvm, incubation time 10-15h; Then with 1-2 DEG C/h rate of temperature fall slow cooling to 10-15 DEG C, mixing speed 250-300r/m, ventilation 1-2vvm, constant temperature culture 15-20h; Continue with 1-2 DEG C/h rate of temperature fall slow cooling to 2-5 DEG C, now, seeding tank liquid seeds is added access fermentation tank with 4% inoculum concentration, constant temperature culture 20-30h; Finally slowly be warming up to 10-15 DEG C, mixing speed 200-400r/m, ventilation 1-2vvm, constant temperature culture 15-20h with 1-2 DEG C/h heating rate; Continue slowly to be warming up to 27-30 DEG C, constant temperature culture 15-20h with 1-2 DEG C/h heating rate; Fermentation liquor is filtered, concentrated, allotment, essence filter, dry solid fungal alpha-amylase.
3. the destarch complex enzyme containing fungal alpha-amylase as claimed in claim 1, it is characterized in that, described mycotic culture thing viable bacteria content is 7x10
10-9x10
10cFU/g.
4. the destarch complex enzyme containing fungal alpha-amylase as claimed in claim 1, it is characterized in that, the preparation method of described malt extract is: by fructus hordei germinatus and wheat malt 6-8:3-5 Homogeneous phase mixing in mass ratio, be crushed to granularity 0.5-1mm, obtain pulverizing Fructus Hordei Germinatus; Add and pulverize malt quality 1-3 water doubly, be 3-4 by lactic acid adjust ph, Microwave Extraction is carried out under power 150-300W, frequency 2000Hz condition, wherein, each microwave irradiation total time 60-80s, carry out compartment irradiation: irradiation 10s, interval 10s, control temperature 20-35 DEG C, irradiation like this 10 times, under power 200-300W, frequency 30-40KHz condition, carry out ultrasonic assistant extraction simultaneously; Insulation 1-3h, then, Microwave Extraction is carried out under power 200-400W, frequency 2000Hz condition, wherein, each microwave irradiation total time 90-105s, carry out compartment irradiation: irradiation 15s, interval 10s, control temperature 40-60 DEG C, irradiation like this 10 times, under power 300-500W, frequency 40-50KHz condition, carry out ultrasonic assistant extraction simultaneously; 55-65 DEG C of insulation 30-60min; Last Temperature fall is to room temperature, and in electric-field intensity 25-35kV/cm, burst length 400-600 μ s, carries out high-pressure pulse electric and extract 15-20min under pulse frequency 200-300Hz condition; Extracting liquid filtering obtains the first filtrate, adds the water rinse of filter residue 2 times, filters to obtain the second filtrate, and by the first filtrate and the second filtrate 1:1-2 Homogeneous phase mixing in mass ratio, namely mixed liquor ultrafiltration concentration, freeze drying, low-temperature grinding obtain malt extract.
5. the destarch complex enzyme containing fungal alpha-amylase as claimed in claim 1, it is characterized in that, the quality component of described non-ionic surface active agent is: alkyl polyglycoside 25-35 part, methyl glucamine 15-25 part, N-dodecyl ethylenediamine triacetic acid sodium 8-12 part, isomery alcohol ether carboxylate AEC-11078-12 part, Lauric Acid Monoethanolamide Ether Carboxylate 5-8 part, peregal c-1253-5 part, JFC 3-5 part.
6. the destarch complex enzyme containing fungal alpha-amylase as claimed in claim 1, is characterized in that, described antioxidant is any one or a few combination in grape pip procyanidin, Rosmarinus officinalis extract and apricot leaf extract.
7. prepare as claimed in claim 1 containing the method for the destarch complex enzyme of fungal alpha-amylase for one kind; it is characterized in that; comprise the steps: first by described protective agent, activator ultramicro grinding respectively; Homogeneous phase mixing; immediately add malt extract, mycotic culture thing Homogeneous phase mixing 20-30min successively; then add fungal alpha-amylase, lipase, non-ionic surface active agent Homogeneous phase mixing successively, finally add antioxidant, after mixing, pack and obtain destarch complex enzyme.
8. the preparation method of the destarch complex enzyme containing fungal alpha-amylase as claimed in claim 7, it is characterized in that, described non-ionic surface active agent is environment-friendly type non-ionic surface active agent.
9. the preparation method of the destarch complex enzyme containing fungal alpha-amylase as claimed in claim 7, it is characterized in that, described antioxidant is grape pip procyanidin, Rosmarinus officinalis extract and apricot leaf extract 5-7:2-4:1-4 Homogeneous phase mixing in mass ratio.
10. the application of destarch complex enzyme in weaving desizing containing fungal alpha-amylase as described in as arbitrary in claim 1-6.
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109452505A (en) * | 2018-12-29 | 2019-03-12 | 广西壮族自治区农业科学院农产品质量安全与检测技术研究所 | A kind of sugar-cane juice preparation method of high Xylose Content |
| CN112832031A (en) * | 2020-03-27 | 2021-05-25 | 中国科学院天津工业生物技术研究所 | Biological compound for improving fabric capillary effect and preparation method and application thereof |
| CN114293299A (en) * | 2021-12-31 | 2022-04-08 | 南通鹏翔织造有限公司 | Spinning process of polylactic acid filament polyester wheel blended double-core composite yarn |
| CN115726186A (en) * | 2021-08-31 | 2023-03-03 | 山东双圆生物科技有限公司 | Waste cloth decolorizing agent containing biological enzyme, preparation method, treating agent and application |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1337488A (en) * | 2000-08-11 | 2002-02-27 | 青岛大学 | Pretreating biological enzyme process for natural cellulosic fibre |
| CN101851614A (en) * | 2010-05-12 | 2010-10-06 | 江苏博立生物制品有限公司 | Process for improving fermentation conversion rate of enzyme preparation |
| US20100278970A1 (en) * | 2007-03-14 | 2010-11-04 | Danisco Us, Inc., Genencor Division | Trichoderma reesei alpha-amylase enhances saccharification of corn starch |
| CN102704258A (en) * | 2012-03-28 | 2012-10-03 | 太仓棨淂服装有限公司 | Water washing process for forming snowflake-like visual effect of clothes |
| CN103276581A (en) * | 2013-06-08 | 2013-09-04 | 太仓市芸芸化纤有限公司 | Composition used for high-density fabric desizing agent |
| CN103628297A (en) * | 2013-12-02 | 2014-03-12 | 湖州金塔地纺织厂 | Fabric desizing agent |
-
2014
- 2014-12-01 CN CN201410718815.3A patent/CN104480690B/en active Active
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1337488A (en) * | 2000-08-11 | 2002-02-27 | 青岛大学 | Pretreating biological enzyme process for natural cellulosic fibre |
| US20100278970A1 (en) * | 2007-03-14 | 2010-11-04 | Danisco Us, Inc., Genencor Division | Trichoderma reesei alpha-amylase enhances saccharification of corn starch |
| CN101851614A (en) * | 2010-05-12 | 2010-10-06 | 江苏博立生物制品有限公司 | Process for improving fermentation conversion rate of enzyme preparation |
| CN102704258A (en) * | 2012-03-28 | 2012-10-03 | 太仓棨淂服装有限公司 | Water washing process for forming snowflake-like visual effect of clothes |
| CN103276581A (en) * | 2013-06-08 | 2013-09-04 | 太仓市芸芸化纤有限公司 | Composition used for high-density fabric desizing agent |
| CN103628297A (en) * | 2013-12-02 | 2014-03-12 | 湖州金塔地纺织厂 | Fabric desizing agent |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109452505A (en) * | 2018-12-29 | 2019-03-12 | 广西壮族自治区农业科学院农产品质量安全与检测技术研究所 | A kind of sugar-cane juice preparation method of high Xylose Content |
| CN112832031A (en) * | 2020-03-27 | 2021-05-25 | 中国科学院天津工业生物技术研究所 | Biological compound for improving fabric capillary effect and preparation method and application thereof |
| CN112832031B (en) * | 2020-03-27 | 2023-02-28 | 中国科学院天津工业生物技术研究所 | Biological compound for improving fabric capillary effect and preparation method and application thereof |
| CN115726186A (en) * | 2021-08-31 | 2023-03-03 | 山东双圆生物科技有限公司 | Waste cloth decolorizing agent containing biological enzyme, preparation method, treating agent and application |
| CN114293299A (en) * | 2021-12-31 | 2022-04-08 | 南通鹏翔织造有限公司 | Spinning process of polylactic acid filament polyester wheel blended double-core composite yarn |
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