CN104480166A - Production of fructo-oligosaccharide by virtue of amylase method - Google Patents
Production of fructo-oligosaccharide by virtue of amylase method Download PDFInfo
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- CN104480166A CN104480166A CN201410726333.2A CN201410726333A CN104480166A CN 104480166 A CN104480166 A CN 104480166A CN 201410726333 A CN201410726333 A CN 201410726333A CN 104480166 A CN104480166 A CN 104480166A
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- 238000000034 method Methods 0.000 title claims abstract description 17
- 238000004519 manufacturing process Methods 0.000 title claims abstract description 15
- 239000004382 Amylase Substances 0.000 title claims abstract description 12
- FTSSQIKWUOOEGC-RULYVFMPSA-N fructooligosaccharide Chemical compound OC[C@H]1O[C@@](CO)(OC[C@@]2(OC[C@@]3(OC[C@@]4(OC[C@@]5(OC[C@@]6(OC[C@@]7(OC[C@@]8(OC[C@@]9(OC[C@@]%10(OC[C@@]%11(O[C@H]%12O[C@H](CO)[C@@H](O)[C@H](O)[C@H]%12O)O[C@H](CO)[C@@H](O)[C@@H]%11O)O[C@H](CO)[C@@H](O)[C@@H]%10O)O[C@H](CO)[C@@H](O)[C@@H]9O)O[C@H](CO)[C@@H](O)[C@@H]8O)O[C@H](CO)[C@@H](O)[C@@H]7O)O[C@H](CO)[C@@H](O)[C@@H]6O)O[C@H](CO)[C@@H](O)[C@@H]5O)O[C@H](CO)[C@@H](O)[C@@H]4O)O[C@H](CO)[C@@H](O)[C@@H]3O)O[C@H](CO)[C@@H](O)[C@@H]2O)[C@@H](O)[C@@H]1O FTSSQIKWUOOEGC-RULYVFMPSA-N 0.000 title abstract 7
- 229940107187 fructooligosaccharide Drugs 0.000 title abstract 7
- 102000013142 Amylases Human genes 0.000 title abstract 2
- 108010065511 Amylases Proteins 0.000 title abstract 2
- 235000019418 amylase Nutrition 0.000 title abstract 2
- 229920002472 Starch Polymers 0.000 claims abstract description 36
- 235000019698 starch Nutrition 0.000 claims abstract description 36
- 239000008107 starch Substances 0.000 claims abstract description 36
- 102000004190 Enzymes Human genes 0.000 claims abstract description 31
- 108090000790 Enzymes Proteins 0.000 claims abstract description 31
- 238000006243 chemical reaction Methods 0.000 claims abstract description 21
- 239000007788 liquid Substances 0.000 claims abstract description 21
- 108010042889 Inulosucrase Proteins 0.000 claims abstract description 16
- 108700040099 Xylose isomerases Proteins 0.000 claims abstract description 16
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 claims abstract description 14
- 229930006000 Sucrose Natural products 0.000 claims abstract description 14
- 239000005720 sucrose Substances 0.000 claims abstract description 14
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Chemical compound OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims description 34
- 239000000872 buffer Substances 0.000 claims description 20
- LOKCTEFSRHRXRJ-UHFFFAOYSA-I dipotassium trisodium dihydrogen phosphate hydrogen phosphate dichloride Chemical compound P(=O)(O)(O)[O-].[K+].P(=O)(O)([O-])[O-].[Na+].[Na+].[Cl-].[K+].[Cl-].[Na+] LOKCTEFSRHRXRJ-UHFFFAOYSA-I 0.000 claims description 20
- 239000002953 phosphate buffered saline Substances 0.000 claims description 20
- 235000000346 sugar Nutrition 0.000 claims description 12
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 claims description 10
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 claims description 10
- 239000012535 impurity Substances 0.000 claims description 10
- 239000000463 material Substances 0.000 claims description 6
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 6
- NWUYHJFMYQTDRP-UHFFFAOYSA-N 1,2-bis(ethenyl)benzene;1-ethenyl-2-ethylbenzene;styrene Chemical compound C=CC1=CC=CC=C1.CCC1=CC=CC=C1C=C.C=CC1=CC=CC=C1C=C NWUYHJFMYQTDRP-UHFFFAOYSA-N 0.000 claims description 5
- ZCYVEMRRCGMTRW-UHFFFAOYSA-N 7553-56-2 Chemical compound [I] ZCYVEMRRCGMTRW-UHFFFAOYSA-N 0.000 claims description 5
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 claims description 5
- 229910052799 carbon Inorganic materials 0.000 claims description 5
- 230000002478 diastatic effect Effects 0.000 claims description 5
- 230000000694 effects Effects 0.000 claims description 5
- 238000001914 filtration Methods 0.000 claims description 5
- 230000002641 glycemic effect Effects 0.000 claims description 5
- 229910017053 inorganic salt Inorganic materials 0.000 claims description 5
- 238000009413 insulation Methods 0.000 claims description 5
- 229910052740 iodine Inorganic materials 0.000 claims description 5
- 239000011630 iodine Substances 0.000 claims description 5
- 239000003456 ion exchange resin Substances 0.000 claims description 5
- 229920003303 ion-exchange polymer Polymers 0.000 claims description 5
- 235000015110 jellies Nutrition 0.000 claims description 5
- 239000008274 jelly Substances 0.000 claims description 5
- 235000013336 milk Nutrition 0.000 claims description 5
- 239000008267 milk Substances 0.000 claims description 5
- 210000004080 milk Anatomy 0.000 claims description 5
- 238000002156 mixing Methods 0.000 claims description 5
- 239000000843 powder Substances 0.000 claims description 5
- 238000005070 sampling Methods 0.000 claims description 5
- 238000002834 transmittance Methods 0.000 claims description 5
- 238000007738 vacuum evaporation Methods 0.000 claims description 5
- 238000010792 warming Methods 0.000 claims description 5
- 230000001105 regulatory effect Effects 0.000 abstract description 2
- 239000008363 phosphate buffer Substances 0.000 abstract 2
- UXVMQQNJUSDDNG-UHFFFAOYSA-L Calcium chloride Chemical compound [Cl-].[Cl-].[Ca+2] UXVMQQNJUSDDNG-UHFFFAOYSA-L 0.000 abstract 1
- 108090000637 alpha-Amylases Proteins 0.000 abstract 1
- 102000004139 alpha-Amylases Human genes 0.000 abstract 1
- 229940024171 alpha-amylase Drugs 0.000 abstract 1
- 239000001110 calcium chloride Substances 0.000 abstract 1
- 229910001628 calcium chloride Inorganic materials 0.000 abstract 1
- 235000011148 calcium chloride Nutrition 0.000 abstract 1
- 230000003247 decreasing effect Effects 0.000 abstract 1
- 229940088598 enzyme Drugs 0.000 abstract 1
- 238000004537 pulping Methods 0.000 abstract 1
- 239000002994 raw material Substances 0.000 abstract 1
- 238000007670 refining Methods 0.000 abstract 1
- RFSUNEUAIZKAJO-ARQDHWQXSA-N Fructose Chemical compound OC[C@H]1O[C@](O)(CO)[C@@H](O)[C@@H]1O RFSUNEUAIZKAJO-ARQDHWQXSA-N 0.000 description 5
- 239000011575 calcium Substances 0.000 description 5
- 239000008103 glucose Substances 0.000 description 4
- 229930091371 Fructose Natural products 0.000 description 3
- 239000005715 Fructose Substances 0.000 description 3
- WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 description 3
- 239000000243 solution Substances 0.000 description 3
- LPQOADBMXVRBNX-UHFFFAOYSA-N ac1ldcw0 Chemical compound Cl.C1CN(C)CCN1C1=C(F)C=C2C(=O)C(C(O)=O)=CN3CCSC1=C32 LPQOADBMXVRBNX-UHFFFAOYSA-N 0.000 description 2
- 230000007812 deficiency Effects 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 239000000203 mixture Substances 0.000 description 2
- 238000000926 separation method Methods 0.000 description 2
- ODEHMIGXGLNAKK-OESPXIITSA-N 6-kestotriose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)OC[C@@H]1[C@@H](O)[C@H](O)[C@](CO)(O[C@@H]2[C@@H]([C@@H](O)[C@H](O)[C@@H](CO)O2)O)O1 ODEHMIGXGLNAKK-OESPXIITSA-N 0.000 description 1
- 241000193830 Bacillus <bacterium> Species 0.000 description 1
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 description 1
- 235000007542 Cichorium intybus Nutrition 0.000 description 1
- 244000298479 Cichorium intybus Species 0.000 description 1
- RFSUNEUAIZKAJO-VRPWFDPXSA-N D-Fructose Natural products OC[C@H]1OC(O)(CO)[C@@H](O)[C@@H]1O RFSUNEUAIZKAJO-VRPWFDPXSA-N 0.000 description 1
- 206010020772 Hypertension Diseases 0.000 description 1
- 208000008589 Obesity Diseases 0.000 description 1
- 240000000111 Saccharum officinarum Species 0.000 description 1
- 235000007201 Saccharum officinarum Nutrition 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 239000000654 additive Substances 0.000 description 1
- 230000000996 additive effect Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
- 229910052791 calcium Inorganic materials 0.000 description 1
- 150000001720 carbohydrates Chemical class 0.000 description 1
- 235000014633 carbohydrates Nutrition 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- HVYWMOMLDIMFJA-DPAQBDIFSA-N cholesterol Chemical compound C1C=C2C[C@@H](O)CC[C@]2(C)[C@@H]2[C@@H]1[C@@H]1CC[C@H]([C@H](C)CCCC(C)C)[C@@]1(C)CC2 HVYWMOMLDIMFJA-DPAQBDIFSA-N 0.000 description 1
- 208000002925 dental caries Diseases 0.000 description 1
- 206010012601 diabetes mellitus Diseases 0.000 description 1
- 235000013399 edible fruits Nutrition 0.000 description 1
- 239000000284 extract Substances 0.000 description 1
- 239000000835 fiber Substances 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 235000003599 food sweetener Nutrition 0.000 description 1
- 125000002791 glucosyl group Chemical group C1([C@H](O)[C@@H](O)[C@H](O)[C@H](O1)CO)* 0.000 description 1
- 230000007062 hydrolysis Effects 0.000 description 1
- 238000006460 hydrolysis reaction Methods 0.000 description 1
- 230000002519 immonomodulatory effect Effects 0.000 description 1
- 230000000968 intestinal effect Effects 0.000 description 1
- 150000002632 lipids Chemical class 0.000 description 1
- 235000020824 obesity Nutrition 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 238000000746 purification Methods 0.000 description 1
- 108091008146 restriction endonucleases Proteins 0.000 description 1
- 239000003765 sweetening agent Substances 0.000 description 1
- UFTFJSFQGQCHQW-UHFFFAOYSA-N triformin Chemical compound O=COCC(OC=O)COC=O UFTFJSFQGQCHQW-UHFFFAOYSA-N 0.000 description 1
Abstract
The invention relates to production of fructo-oligosaccharide by virtue of an amylase method. The production comprises the following steps: (1) pulping and liquefying: adding 6-10U/g of alpha-amylase to each ton of starch raw materials, adding CaCl2, and continuing a liquefying reaction for 10min; (2) saccharifying: leading liquefied starch to a saccharifying tank, decreasing temperature to 60 DEG C and regulating pH to 4.5, and adding 250U/g of starch saccharifying enzyme; (3) refining saccharifying liquid; and (4) preparing fructo-oligosaccharide by virtue of an enzymic method: completely dissolving 20g of sucrose to 80.0ml of a phosphate buffer, adding fructosyltransferase and glucose isomerase, adding the phosphate buffer, and reacting at 50 DEG C so as to obtain the fructo-oligosaccharide. The production of the fructo-oligosaccharide disclosed by the invention is simple to operate, mild in action condition and easy to master; the fructo-oligosaccharide is high in purity which is more than 98%; the entire process can be completed within 100-160min; the production is a great innovation in high-purity fructo-oligosaccharide, and is worthy of popularizing.
Description
(1) technical field
The present invention relates to oligofructose, be specifically related to a kind of starch Production by Enzymes oligofructose.
(2) background technology
Oligofructose refers to that 2 ~ 5 fructosyls are chain link, the end group being chain with a glucosyl group, with fructosyl → fructose connecting key for main body framework links the carbohydrate formed.Namely refer to that 1 ~ 4 fructosyl is connected to the mixture of kestose (GF2), GF3 (GF3), GF4 (GF4) and sugarcane fruit six sugar (GF5) that the D-Fructose base of sucrose is formed with β-2,1 key.Commodity oligofructose is generally also containing a small amount of sucrose, fructose, glucose.Oligofructose is a kind of natural active matter, has regulating intestinal canal flora, propagation bifidus bacillus, promote the absorption of calcium, adjusting blood lipid, immunomodulatory, the novel sweetener of the nourishing functions such as anti-dental caries, is described as the additive of new generation of most potentiality after the microbiotic epoch---growth-promoting material; Oligofructose or a kind of water miscible food fibre, can reduce serum cholesterol and content of triglyceride, takes in oligofructose and glucose level can not be caused to fluctuate, can be used as the sweeting agent of hypertension, diabetes and obesity patient.
At present, the production method of oligofructose mainly contains two kinds: 1. sucrose inversion method, this method in 1982 at Japanese industry, with the sucrose solution of ferment treatment high density, obtain the solution of oligofructose contents on dry basis 55%, all the other 45% be fructose, glucose sugar and sucrose, obtain through separation and purification the oligofructose that purity is 95%.This method due to separating-purifying difficulty large, Chinese Enterprise can only produce the oligofructose product of 55% substantially.2. synanthrin hydrolysis method, this method is adopted in a large number by Europe at present.This method extracts synanthrin from witloof, be hydrolyzed through synanthrin restriction endonuclease, obtain the mixture of oligofructose, glucose, sucrose, oligofructose is obtained through separating-purifying, but because in enzymolysis product, the contents on dry basis of oligofructose also only has 55%, monose and sucrose content are too high, separation difficulty, can't produce the product that oligofructose content is greater than 95%.
There is same deficiency in above two kinds of technology, namely producing enzyme used only has few unit to produce, and as the then more difficult preparation of pure enzyme, use enzyme-squash techniqued oligofructose, cost is high, enzymolysis product be difficult to control, monose and sucrose content high, separating-purifying difficulty is large.
(3) summary of the invention
The present invention, in order to make up the deficiencies in the prior art, provides a kind of starch Production by Enzymes oligofructose, easy to operate, and process is easy to control, and facilitate large-scale industrial to produce, oligofructose purity is high, can reach more than 98%.
The present invention is achieved through the following technical solutions:
A kind of starch Production by Enzymes oligofructose, its special character is: comprise the following steps:
(1) size mixing and liquefaction: starch water is modulated into the starch milk of dry matter content 30%-35%, adjusts pH6.0-6.5 with hydrochloric acid, starch material per ton adds ɑ-amylase consumption 6-10U/g starch, adds CaCl
2regulate Ca
2+concentration reaches 0.01mol/l, and powder stock pump enters Jet liquefier, is instantaneously warming up to 105-110 DEG C, pipeline liquefaction reaction 10-15min, feed liquid is delivered to liquefied pot, at 95-97 DEG C of temperature, add ɑ-amylase at twice, continue liquefaction reaction 10min, iodine colour response is qualified;
(2) saccharification: liquefied starch introduces saccharifying tank, is cooled to 60 DEG C of adjustment pH to 4.5, adds 250U/g diastatic enzyme, under gap is stirred, 60 DEG C of insulation 30min, saccharification is to DE > 95, heat to 90 DEG C, saccharifying enzyme is destroyed, saccharification react is stopped;
(3) saccharified liquid is refined: adopt the continuous filtration of diatomite precoating rotary drum filter, remove impurity and the jelly of non-solubility in saccharified liquid, use activated carbon decolorizing subsequently, the inorganic salt in spent ion exchange resin removing liquid glucose and organic impurity, improve purity further, colourless or the faint yellow sugar-containing concentration 24% of liquid glucose, specific conductivity < 50MS/cm, pH4.5-5.0, vacuum evaporation is to transmittance more than 90%, DE value 96-97, sugar concentration is 40%-45%;
(4) enzyme process prepares oligofructose: first by 20 grams of sucrose, be dissolved in 80.0ml phosphate buffered saline buffer completely, then fructosyl transferase and glucose isomerase is added, fructosyl transferase and glucose isomerase liquid glucose: the weight ratio of liquid glucose is 1:7, and wherein the enzyme activity ratio of fructosyl transferase and glucose isomerase is 2:1, be added in liquid glucose, reaction 5min, then add phosphate buffered saline buffer, liquid glucose: the volume ratio 100:1 of phosphate buffered saline buffer, in phosphate buffered saline buffer, KH
2pO
4with K
2hPO
4volume ratio is 2:1, and wherein both concentration is 0.05mol/L; Add incitant MgSO
47H
2o, ZnSO
4, Na
2seO
3as incitant, every glycemic liquid 4gMgSO
47H
2o, 1g ZnSO
4, 4g Na
2seO
3, in 50 °, reaction 20min, after 2min is boiled in sampling, is cooled to rapidly less than 40 DEG C, in the centrifugal 5min of 10000r/min, obtains oligofructose.
Beneficial effect of the present invention: the present invention is simple to operate for the production of oligofructose, action condition is gentle, easily grasp, oligofructose purity is high, can reach more than 98%, and whole process 100-160min can complete, be innovation great on high-purity fructo oligosaccharides, be worthy to be popularized.
(4) embodiment
Embodiment 1
The starch Production by Enzymes oligofructose of the present embodiment the steps include:
(1) size mixing and liquefaction: starch water is modulated into the starch milk of dry matter content 30%, adjusts pH6.0 with hydrochloric acid, starch material per ton adds ɑ-amylase consumption 6U/g starch, adds CaCl
2regulate Ca
2+concentration reaches 0.01mol/l, and powder stock pump enters Jet liquefier, is instantaneously warming up to 105 DEG C, pipeline liquefaction reaction 10min, and feed liquid is delivered to liquefied pot, at 95 DEG C of temperature, adds ɑ-amylase at twice, and continue liquefaction reaction 10min, iodine colour response is qualified;
(2) saccharification: liquefied starch introduces saccharifying tank, is cooled to 60 DEG C of adjustment pH to 4.5, adds 250U/g diastatic enzyme, under gap is stirred, 60 DEG C of insulation 30min, saccharification is to DE > 95, heat to 90 DEG C, saccharifying enzyme is destroyed, saccharification react is stopped;
(3) saccharified liquid is refined: adopt the continuous filtration of diatomite precoating rotary drum filter, remove impurity and the jelly of non-solubility in saccharified liquid, use activated carbon decolorizing subsequently, the inorganic salt in spent ion exchange resin removing liquid glucose and organic impurity, improve purity further, colourless or the faint yellow sugar-containing concentration 24% of liquid glucose, specific conductivity < 50MS/cm, pH4.5-5.0, vacuum evaporation is to transmittance more than 90%, DE value 96, sugar concentration is 40%%;
(4) enzyme process prepares oligofructose: first by 20 grams of sucrose, be dissolved in 80.0ml phosphate buffered saline buffer completely, then fructosyl transferase and glucose isomerase is added, fructosyl transferase and glucose isomerase liquid glucose: the weight ratio of liquid glucose is 1:7, and wherein the enzyme activity ratio of fructosyl transferase and glucose isomerase is 2:1, be added in liquid glucose, reaction 5min, then add phosphate buffered saline buffer, liquid glucose: the volume ratio 100:1 of phosphate buffered saline buffer, in phosphate buffered saline buffer, KH
2pO
4with K
2hPO
4volume ratio is 2:1, and wherein both concentration is 0.05mol/L; Add incitant MgSO
47H
2o, ZnSO
4, Na
2seO
3as incitant, every glycemic liquid 4gMgSO
47H
2o, 1g ZnSO
4, 4g Na
2seO
3, in 50 °, reaction 20min, after 2min is boiled in sampling, is cooled to rapidly less than 40 DEG C, in the centrifugal 5min of 10000r/min, obtains oligofructose.
Embodiment 2
The starch Production by Enzymes oligofructose of the present embodiment the steps include:
(1) size mixing and liquefaction: starch water is modulated into the starch milk of dry matter content 35%, adjusts pH6.5 with hydrochloric acid, starch material per ton adds ɑ-amylase consumption 10U/g starch, adds CaCl
2regulate Ca
2+concentration reaches 0.01mol/l, and powder stock pump enters Jet liquefier, is instantaneously warming up to 110 DEG C, pipeline liquefaction reaction 15min, and feed liquid is delivered to liquefied pot, at 97 DEG C of temperature, adds ɑ-amylase at twice, and continue liquefaction reaction 10min, iodine colour response is qualified;
(2) saccharification: liquefied starch introduces saccharifying tank, is cooled to 60 DEG C of adjustment pH to 4.5, adds 250U/g diastatic enzyme, under gap is stirred, 60 DEG C of insulation 30min, saccharification is to DE > 95, heat to 90 DEG C, saccharifying enzyme is destroyed, saccharification react is stopped;
(3) saccharified liquid is refined: adopt the continuous filtration of diatomite precoating rotary drum filter, remove impurity and the jelly of non-solubility in saccharified liquid, use activated carbon decolorizing subsequently, the inorganic salt in spent ion exchange resin removing liquid glucose and organic impurity, improve purity further, colourless or the faint yellow sugar-containing concentration 24% of liquid glucose, specific conductivity < 50MS/cm, pH4.5-5.0, vacuum evaporation is to transmittance more than 90%, DE value 96-97, sugar concentration is 40%-45%;
(4) enzyme process prepares oligofructose: first by 20 grams of sucrose, be dissolved in 80.0ml phosphate buffered saline buffer completely, then fructosyl transferase and glucose isomerase is added, fructosyl transferase and glucose isomerase liquid glucose: the weight ratio of liquid glucose is 1:7, and wherein the enzyme activity ratio of fructosyl transferase and glucose isomerase is 2:1, be added in liquid glucose, reaction 5min, then add phosphate buffered saline buffer, liquid glucose: the volume ratio 100:1 of phosphate buffered saline buffer, in phosphate buffered saline buffer, KH
2pO
4with K
2hPO
4volume ratio is 2:1, and wherein both concentration is 0.05mol/L; Add incitant MgSO
47H
2o, ZnSO
4, Na
2seO
3as incitant, every glycemic liquid 4gMgSO
47H
2o, 1g ZnSO
4, 4g Na
2seO
3, in 50 °, reaction 20min, after 2min is boiled in sampling, is cooled to rapidly less than 40 DEG C, in the centrifugal 5min of 10000r/min, obtains oligofructose.
Embodiment 3
The starch Production by Enzymes oligofructose of the present embodiment the steps include:
(1) size mixing and liquefaction: starch water is modulated into the starch milk of dry matter content 33%, adjusts pH6.0-6.5 with hydrochloric acid, starch material per ton adds ɑ-amylase consumption 8U/g starch, adds CaCl
2regulate Ca
2+concentration reaches 0.01mol/l, and powder stock pump enters Jet liquefier, is instantaneously warming up to 105-110 DEG C, pipeline liquefaction reaction 13min, feed liquid is delivered to liquefied pot, at 95-97 DEG C of temperature, add ɑ-amylase at twice, continue liquefaction reaction 10min, iodine colour response is qualified;
(2) saccharification: liquefied starch introduces saccharifying tank, is cooled to 60 DEG C of adjustment pH to 4.5, adds 250U/g diastatic enzyme, under gap is stirred, 60 DEG C of insulation 30min, saccharification is to DE > 95, heat to 90 DEG C, saccharifying enzyme is destroyed, saccharification react is stopped;
(3) saccharified liquid is refined: adopt the continuous filtration of diatomite precoating rotary drum filter, remove impurity and the jelly of non-solubility in saccharified liquid, use activated carbon decolorizing subsequently, the inorganic salt in spent ion exchange resin removing liquid glucose and organic impurity, improve purity further, colourless or the faint yellow sugar-containing concentration 24% of liquid glucose, specific conductivity < 50MS/cm, pH4.5-5.0, vacuum evaporation is to transmittance more than 90%, DE value 96-97, sugar concentration is 40%-45%;
(4) enzyme process prepares oligofructose: first by 20 grams of sucrose, be dissolved in 80.0ml phosphate buffered saline buffer completely, then fructosyl transferase and glucose isomerase is added, fructosyl transferase and glucose isomerase liquid glucose: the weight ratio of liquid glucose is 1:7, and wherein the enzyme activity ratio of fructosyl transferase and glucose isomerase is 2:1, be added in liquid glucose, reaction 5min, then add phosphate buffered saline buffer, liquid glucose: the volume ratio 100:1 of phosphate buffered saline buffer, in phosphate buffered saline buffer, KH
2pO
4with K
2hPO
4volume ratio is 2:1, and wherein both concentration is 0.05mol/L; Add incitant MgSO
47H
2o, ZnSO
4, Na
2seO
3as incitant, every glycemic liquid 4gMgSO
47H
2o, 1g ZnSO
4, 4g Na
2seO
3, in 50 °, reaction 20min, after 2min is boiled in sampling, is cooled to rapidly less than 40 DEG C, in the centrifugal 5min of 10000r/min, obtains oligofructose.
Claims (1)
1. a starch Production by Enzymes oligofructose, is characterized in that: comprise the following steps:
(1) size mixing and liquefaction: starch water is modulated into the starch milk of dry matter content 30%-35%, adjusts pH6.0-6.5 with hydrochloric acid, starch material per ton adds ɑ-amylase consumption 6-10U/g starch, adds CaCl
2regulate Ca
2+concentration reaches 0.01mol/l, and powder stock pump enters Jet liquefier, is instantaneously warming up to 105-110 DEG C, pipeline liquefaction reaction 10-15min, feed liquid is delivered to liquefied pot, at 95-97 DEG C of temperature, add ɑ-amylase at twice, continue liquefaction reaction 10min, iodine colour response is qualified;
(2) saccharification: liquefied starch introduces saccharifying tank, is cooled to 60 DEG C of adjustment pH to 4.5, adds 250U/g diastatic enzyme, under gap is stirred, 60 DEG C of insulation 30min, saccharification is to DE > 95, heat to 90 DEG C, saccharifying enzyme is destroyed, saccharification react is stopped;
(3) saccharified liquid is refined: adopt the continuous filtration of diatomite precoating rotary drum filter, remove impurity and the jelly of non-solubility in saccharified liquid, use activated carbon decolorizing subsequently, the inorganic salt in spent ion exchange resin removing liquid glucose and organic impurity, improve purity further, colourless or the faint yellow sugar-containing concentration 24% of liquid glucose, specific conductivity < 50MS/cm, pH4.5-5.0, vacuum evaporation is to transmittance more than 90%, DE value 96-97, sugar concentration is 40%-45%;
(4) enzyme process prepares oligofructose: first by 20 grams of sucrose, be dissolved in 80.0ml phosphate buffered saline buffer completely, then fructosyl transferase and glucose isomerase is added, fructosyl transferase and glucose isomerase liquid glucose: the weight ratio of liquid glucose is 1:7, and wherein the enzyme activity ratio of fructosyl transferase and glucose isomerase is 2:1, be added in liquid glucose, reaction 5min, then add phosphate buffered saline buffer, liquid glucose: the volume ratio 100:1 of phosphate buffered saline buffer, in phosphate buffered saline buffer, KH
2pO
4with K
2hPO
4volume ratio is 2:1, and wherein both concentration is 0.05mol/L; Add incitant MgSO
47H
2o, ZnSO
4, Na
2seO
3as incitant, every glycemic liquid 4gMgSO
47H
2o, 1g ZnSO
4, 4g Na
2seO
3, in 50 °, reaction 20min, after 2min is boiled in sampling, is cooled to rapidly less than 40 DEG C, in the centrifugal 5min of 10000r/min, obtains oligofructose.
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| CN105106043A (en) * | 2015-07-16 | 2015-12-02 | 山东省分析测试中心 | Peony flower-containing anti-inflammation and deodorization fragrant mouthwash and preparation method thereof |
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| CN115109810A (en) * | 2022-07-22 | 2022-09-27 | 金建国 | Fructo-oligosaccharide and preparation method thereof |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN105106043A (en) * | 2015-07-16 | 2015-12-02 | 山东省分析测试中心 | Peony flower-containing anti-inflammation and deodorization fragrant mouthwash and preparation method thereof |
| CN105106043B (en) * | 2015-07-16 | 2017-10-20 | 山东省分析测试中心 | Fragrant mouthwash of a kind of peony anti-inflammatory deodorization and preparation method thereof |
| CN110106215A (en) * | 2019-05-10 | 2019-08-09 | 武汉友谊兴泰科技有限公司 | Saccharification, filtering technique and its equipment in a kind of fructose production process |
| CN115109810A (en) * | 2022-07-22 | 2022-09-27 | 金建国 | Fructo-oligosaccharide and preparation method thereof |
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