CN101724669A - Method for producing moderately inverted syrup by synergistic saccharification with multiple enzymes - Google Patents
Method for producing moderately inverted syrup by synergistic saccharification with multiple enzymes Download PDFInfo
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- CN101724669A CN101724669A CN200910230559A CN200910230559A CN101724669A CN 101724669 A CN101724669 A CN 101724669A CN 200910230559 A CN200910230559 A CN 200910230559A CN 200910230559 A CN200910230559 A CN 200910230559A CN 101724669 A CN101724669 A CN 101724669A
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Abstract
The invention relates to a method for producing moderately inverted syrup by synergistic saccharification with multiple enzymes, comprising the following steps: liquifying raw maerial of high-temperature corn starch with high-temperature amylase, saccharifying with saccharifying enzyme and barley Belta amylase, filtering, neutralizing, decolorizing, exchanging ions, evaporating and concentrating to obtain the moderately inverted syrup. The dextrose equivalent (DE) value of the liquified liquid is controlled in the high range, and the saccharifying temperature, the pH value and the enzyme dosage need to be controlled strictly in the saccharifying process to ensure the components of sugar in the liquid material. The method has mild production condition and low cost, is convenient to operate, generates greatly reduced pollutants and can fully replace the traditional method for producing the moderately inverted syrup with acid.
Description
Technical field
The invention belongs to a kind of preparation method of Dian Fentang, specifically, relate in particular to a kind of the utilization in the multienzyme synergism saccharification preparation and transform syrupy method.
Background technology
In transform syrupy DE value between 38～42%, be the widely used a kind of raw material of confection and other foodstuffs industry, also be the starch syrup production history at most, a kind of syrup of output maximum.China mainly adopts the production of acid system hydrolysis process.For a long time, the syrupy color and luster of middle conversion that uses the acid system hydrolysis process to prepare is dark, impurity is more, make with extra care difficulty and production process can cause environmental pollution.At present, also do not have a kind of can prepare quality preferably in invert syrup, and in preparation process, can not cause the preparation method of environmental pollution.
Summary of the invention
The object of the present invention is to provide a kind ofly, the defective that the invert syrup color and luster is dark in the preparation in the prior art, impurity is more to solve, refining difficulty and production process can cause environmental pollution to transform syrupy method in the multienzyme synergism saccharification preparation.
The invention provides a kind ofly to transform syrupy method in the multienzyme synergism saccharification preparation, this method comprises the steps:
(1) starch liquefacation: 1. the W-Gum powder slurry concentration is controlled at 17～20 degree Beaume, regulates the powder slurry with alkali and makes its pH value between 5.3～5.8, preferably uses Na
2CO
3Regulate; 2. adding alpha-amylase stirs, carry out steam ejection liquefaction then, liquefaction temperature is controlled at 90～110 ℃, keep for some time control DE value between 22～28%, carry out the high temperature enzyme that goes out, temperature is at 130～140 ℃, and this process obtains feed liquid A, the concentration of feed liquid A preferably is controlled at 35～40wt%, and sugared composition is: glucose 1～5%, maltose 4～8%, trisaccharide maltose 6～10%;
(2) saccharification: 1. the temperature of feed liquid A is dropped to 50～70 ℃ and add acid for adjusting pH value between 4.2～5.0, squeeze in the saccharifying tank with pump then, preferably regulate with sulfuric acid or hydrochloric acid; 2. add zymin and carry out saccharification, zymin is fungal enzyme or barley beta-amylase preferably, and its addition is: saccharifying enzyme 30～80ml/t dry starch, fungal enzyme 5～20ml/t dry starch, barley beta-amylase 10～50ml/t dry starch; 3. saccharification time is controlled between 35～45h, saccharification DE value is controlled between 38～42%, this moment, sugared composition was similar to acid system sugar, this process obtains feed liquid B, the pH best values of feed liquid B is 4.5～5.0, and sugared composition is: glucose 15～20%, maltose 12～16%, trisaccharide maltose 11～15%;
(3) filter: feed liquid B filter cleaner is obtained feed liquid C;
(4) neutralization decolouring: feed liquid C placed adds the gac decolouring that neutralizes in the neutralization tank and obtain feed liquid D, in and bleaching temperature be preferably 70～80 ℃, the gac addition is preferably 1.5～2.5kg/t dry, bleaching time is preferably 15～60min;
(5) ion-exchange: feed liquid D is carried out ion-exchange by resin obtain feed liquid E, the concentration of feed liquid D is preferably 38～42wt%, temperature is 40～45 ℃;
(6) evaporation concentration: feed liquid E is carried out evaporation concentration obtain feed liquid F, the concentration of feed liquid F is preferably 70～80wt%.
Technical characterstic of the present invention is: the DE value of a liquefier is 22～28%, the higher relatively zymin addition that reduces the later stage saccharification of DE value, thus reduce production costs; The b liquefier will be through the secondary high-temperature injection enzyme that goes out, and the amylase inactivation lays the foundation for the saccharification in step down; C adopts the method for plurality of enzymes cooperative saccharification, the reaction conditions gentleness, and the middle invert syrup of making can substitute the acid system syrup fully and be used for various food mfgs, and the pollutent of generation significantly reduces, and is easy and simple to handle, reduced cost; D the present invention is to be raw material with the W-Gum; through high temperature liquefaction, saccharification, refining, technology such as concentrate and make, this technological reaction mild condition, easy and simple to handle; limpid in sight and the sewage that produce of products obtained therefrom reduces greatly and helps environment protection, has great economic benefit and social benefit.
Embodiment
Can further be well understood to the present invention by the following specific embodiments of the invention that provide.But they are not limitation of the invention.
Except as otherwise noted, the percentage ratio that is adopted among the present invention is mass percent.
Embodiment 1
Concrete step is as follows:
(1) starch liquefacation: 1. the W-Gum powder slurry concentration is controlled at 17.5 degree Beaume, and regulating powder slurry pH value with alkali is 5.4; 2. add alpha-amylase and stir, carry out steam ejection liquefaction then, liquefaction temperature is at 95 ℃, and the DE value is 23.5%, liquefier sugar composition: glucose 1.4%, maltose 6.6%, trisaccharide maltose 8.5%; Carry out the high temperature enzyme that goes out then, 135 ℃ of enzyme-removal temperatures, this process obtains feed liquid A;
(2) saccharification: 1. the temperature of feed liquid A is dropped to 54 ℃ and to add acid for adjusting pH value be 4.6, squeeze in the saccharifying tank with pump then; 2. add saccharifying enzyme, fungal enzyme and barley beta-amylase and carry out saccharification; Its addition is: saccharifying enzyme 35ml/t dry starch, fungal enzyme 10ml/t dry starch, barley beta-amylase 15ml/t dry starch; 3. saccharification time is controlled at 37h, saccharification DE value 38.7%; This process obtains sugared composition: the feed liquid B of glucose 17.3%, maltose 13.2%, trisaccharide maltose 12.4%;
(3) filter: with the feed liquid B filter cleaner of step (2), this process obtains feed liquid C;
(4) neutralization decolouring: the feed liquid C in the step (3) is placed interpolation gac 1.7kg/t dry in the neutralization tank, be warming up to 73 ℃ of neutralization decolouring 20min, this process obtains feed liquid D;
(5) ion-exchange: the feed liquid D temperature in the step (4) is reduced to 39 ℃ carry out ion-exchange, obtain the feed liquid E that concentration is 41wt% by resin;
(6) evaporation concentration: with the feed liquid E evaporation concentration in the step (5) to concentration is 72.4wt%, and this process obtains feed liquid F, is middle invert syrup.
Embodiment 2
Concrete step is as follows:
(1) starch liquefacation: 1. the W-Gum powder slurry concentration is controlled at 18 degree Beaume, and regulating powder slurry pH value with alkali is 5.5; 2. add alpha-amylase and stir, carry out steam ejection liquefaction then, liquefaction temperature is at 100 ℃, and the DE value is 24.5%, liquefier sugar composition: glucose 2.2%, maltose 7.3%, trisaccharide maltose 8.9%; Carry out the high temperature enzyme that goes out then, enzyme-removal temperature is 137 ℃, and this process obtains feed liquid A;
(2) saccharification: 1. the temperature of feed liquid A is dropped to 56 ℃ and to add acid for adjusting pH value be 4.7, squeeze in the saccharifying tank with pump then; 2. add saccharifying enzyme, fungal enzyme and barley beta-amylase and carry out saccharification; Its addition is: saccharifying enzyme 40ml/t dry starch, fungal enzyme 15ml/t dry starch, barley beta-amylase 25ml/t dry starch; 3. saccharification time is controlled at 38h, and saccharification DE value is 39.3%; This process obtains sugared composition: the feed liquid B of glucose 17.9%, maltose 13.7%, trisaccharide maltose 12.8%;
(3) filter: the feed liquid B filter cleaner with in the step (2) obtains feed liquid C;
(4) neutralization decolouring: the feed liquid C in the step (3) is placed interpolation gac 2.0kg/t dry in the neutralization tank, be warming up to 75 ℃ of neutralization decolouring 35min, this process obtains feed liquid D;
(5) ion-exchange: the temperature of feed liquid D in the step (4) is reduced to 40 ℃ carry out ion-exchange, obtain the feed liquid E that concentration is 41.8wt% by resin;
(6) evaporation concentration: with the feed liquid E evaporation concentration in the step (5) to concentration is 75.3wt%, and this process obtains feed liquid F, is middle invert syrup.
Embodiment 3
Concrete step is as follows:
(1) starch liquefacation: 1. the W-Gum powder slurry concentration is controlled at 19.5 degree Beaume, and regulating powder slurry pH value with alkali is 5.7; 2. add alpha-amylase and stir, carry out steam ejection liquefaction then, liquefaction temperature is at 105 ℃, and the DE value is 25.7%, liquefier sugar composition: glucose 3.2%, maltose 8.0%, trisaccharide maltose 9.9%; Carry out the high temperature enzyme that goes out then, enzyme-removal temperature is 139 ℃, and this process obtains feed liquid A;
(2) saccharification: 1. the temperature of feed liquid A is dropped to 58 ℃ and to add acid for adjusting pH value be 4.9, squeeze in the saccharifying tank with pump then; 2. add saccharifying enzyme, fungal enzyme and barley beta-amylase and carry out saccharification; Its addition is: saccharifying enzyme 50ml/t dry starch, fungal enzyme 20ml/t dry starch, barley beta-amylase 40ml/t dry starch; 3. saccharification time is controlled at 40h, and saccharification DE value is 41.3%; This process obtains sugared composition: the feed liquid B of glucose 18.6%, maltose 14.5%, trisaccharide maltose 13.1%;
(3) filter: the feed liquid B filter cleaner with in the step (2) obtains feed liquid C;
(4) neutralization decolouring: the feed liquid C in the step (3) is placed interpolation gac 2.3kg/t dry in the neutralization tank, be warming up to 78 ℃ of neutralization decolouring 40min, this process obtains feed liquid D;
(5) ion-exchange: the temperature of feed liquid D in the step (4) is reduced to 42 ℃ carry out ion-exchange, obtain the feed liquid E that concentration is 43.8wt% by resin;
(6) evaporation concentration: with the feed liquid E evaporation concentration in the step (5) to concentration is 76.8wt%, and this process obtains feed liquid F, is middle invert syrup.
Claims (9)
1. one kind to transform syrupy method in the multienzyme synergism saccharification preparation, and this method comprises the steps:
(1) starch liquefacation: 1. the W-Gum powder slurry concentration is controlled at 17～20 degree Beaume, regulates the powder slurry with alkali and makes its pH value between 5.3～5.8; 2. add alpha-amylase and stir, carry out steam ejection liquefaction then, liquefaction temperature is controlled at 90～110 ℃, keeps for some time control DE value between 22～28%, carries out the high temperature enzyme that goes out, and temperature is at 130～140 ℃, and this process obtains feed liquid A;
(2) saccharification: 1. the temperature of feed liquid A is dropped to 50～70 ℃ and add acid for adjusting pH value between 4.2～5.0, squeeze in the saccharifying tank with pump then; 2. add zymin and carry out saccharification; 3. saccharification time is controlled between 35～45h, and saccharification DE value is controlled between 38～42%, and this moment, sugared composition was similar to acid system sugar, and this process obtains feed liquid B;
(3) filter: feed liquid B filter cleaner is obtained feed liquid C;
(4) neutralization decolouring: feed liquid C placed add the gac decolouring that neutralizes in the neutralization tank and obtain feed liquid D;
(5) ion-exchange: feed liquid D is carried out ion-exchange by resin obtain feed liquid E;
(6) evaporation concentration: feed liquid E is carried out evaporation concentration obtain feed liquid F, feed liquid F is middle invert syrup.
2. according to claim 1 to transform syrupy method in the multienzyme synergism saccharification preparation, it is characterized in that: the alkali in the described step (1) is Na
2CO
3
3. according to claim 1 to transform syrupy method in the multienzyme synergism saccharification preparation, it is characterized in that: the concentration of feed liquid A is controlled at 35～40wt% in the described step (1), and sugared composition is: glucose 1～5%, maltose 4～8%, trisaccharide maltose 6～10%.
4. according to claim 1 to transform syrupy method in the multienzyme synergism saccharification preparation, it is characterized in that: the acid in the described step (2) is sulfuric acid or hydrochloric acid.
5. according to claim 1 to transform syrupy method in the multienzyme synergism saccharification preparation, it is characterized in that: the zymin in the described step (2) is fungal enzyme or barley beta-amylase, its addition is: saccharifying enzyme 30～80ml/t dry starch, fungal enzyme 5～20ml/t dry starch, barley beta-amylase 10～50ml/t dry starch.
6. according to claim 1 to transform syrupy method in the multienzyme synergism saccharification preparation, it is characterized in that: the pH value of feed liquid B is 4.5～5.0 in the described step (2), and sugared composition is: glucose 15～20%, maltose 12～16%, trisaccharide maltose 11～15%.
7. according to claim 1 to transform syrupy method in the multienzyme synergism saccharification preparation, it is characterized in that: in the described step (4) and bleaching temperature be 70～80 ℃, the gac addition is 1.5～2.5kg/t dry, bleaching time is 15～60min.
8. according to claim 1 anti-with transforming syrupy method in the saccharification preparation with multienzyme, it is characterized in that: the concentration of feed liquid D is 38～42wt% in the described step (5), and temperature is 40～45 ℃.
9. according to claim 1 to transform syrupy method in the multienzyme synergism saccharification preparation, it is characterized in that: the concentration of feed liquid F is 70～80wt% in the described step (6).
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Cited By (10)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN102417918A (en) * | 2011-11-08 | 2012-04-18 | 中粮生物化学（安徽）股份有限公司 | Method for improving quality of sugar solution prepared with corn wet sugar preparation technology |
| CN102492682A (en) * | 2011-12-01 | 2012-06-13 | 甘肃昆仑生化有限责任公司 | Enzyme-killing method of liquefied starch milk during maltodextrin production process |
| CN102876758A (en) * | 2012-09-28 | 2013-01-16 | 浙江华康药业股份有限公司 | Method for preparing fructose syrup |
| CN103146780A (en) * | 2011-12-07 | 2013-06-12 | 长春大学 | Method of simultaneously liquefying and saccharifying cirn starch |
| CN103160551A (en) * | 2013-04-06 | 2013-06-19 | 肇庆焕发生物科技有限公司 | Method for preparing special syrup specially used for glutinous rice cake |
| CN105211463A (en) * | 2015-10-30 | 2016-01-06 | 武汉友谊兴泰淀粉工程有限公司 | A kind of particular components Fructus Hordei Germinatus maltose production method |
| CN105238885A (en) * | 2015-11-10 | 2016-01-13 | 苏州尚融生物技术有限公司 | Preparation method of low-conversion syrup of saccharose |
| CN109430488A (en) * | 2018-11-26 | 2019-03-08 | 广州双桥（重庆）有限公司 | A kind of candied cake of popped rice syrup processing method |
| CN110885723A (en) * | 2019-12-21 | 2020-03-17 | 双桥（厦门）有限公司 | Method for preparing beer syrup based on corn starch |
| CN112375793A (en) * | 2020-11-27 | 2021-02-19 | 广州双桥（重庆）有限公司 | Preparation method of starch syrup for brewing soy sauce |
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| CN102417918B (en) * | 2011-11-08 | 2014-10-22 | 中粮生物化学（安徽）股份有限公司 | Method for improving quality of sugar solution prepared with corn wet sugar preparation technology |
| CN102417918A (en) * | 2011-11-08 | 2012-04-18 | 中粮生物化学（安徽）股份有限公司 | Method for improving quality of sugar solution prepared with corn wet sugar preparation technology |
| CN102492682B (en) * | 2011-12-01 | 2014-07-16 | 甘肃昆仑生化有限责任公司 | Enzyme-killing method of liquefied starch milk during maltodextrin production process |
| CN102492682A (en) * | 2011-12-01 | 2012-06-13 | 甘肃昆仑生化有限责任公司 | Enzyme-killing method of liquefied starch milk during maltodextrin production process |
| CN103146780A (en) * | 2011-12-07 | 2013-06-12 | 长春大学 | Method of simultaneously liquefying and saccharifying cirn starch |
| CN102876758A (en) * | 2012-09-28 | 2013-01-16 | 浙江华康药业股份有限公司 | Method for preparing fructose syrup |
| CN103160551A (en) * | 2013-04-06 | 2013-06-19 | 肇庆焕发生物科技有限公司 | Method for preparing special syrup specially used for glutinous rice cake |
| CN103160551B (en) * | 2013-04-06 | 2015-06-24 | 肇庆焕发生物科技有限公司 | Method for preparing special syrup specially used for glutinous rice cake |
| CN105211463A (en) * | 2015-10-30 | 2016-01-06 | 武汉友谊兴泰淀粉工程有限公司 | A kind of particular components Fructus Hordei Germinatus maltose production method |
| CN105211463B (en) * | 2015-10-30 | 2019-01-01 | 武汉友谊兴泰淀粉工程有限公司 | A kind of malt maltose production method |
| CN105238885A (en) * | 2015-11-10 | 2016-01-13 | 苏州尚融生物技术有限公司 | Preparation method of low-conversion syrup of saccharose |
| CN109430488A (en) * | 2018-11-26 | 2019-03-08 | 广州双桥（重庆）有限公司 | A kind of candied cake of popped rice syrup processing method |
| CN110885723A (en) * | 2019-12-21 | 2020-03-17 | 双桥（厦门）有限公司 | Method for preparing beer syrup based on corn starch |
| CN112375793A (en) * | 2020-11-27 | 2021-02-19 | 广州双桥（重庆）有限公司 | Preparation method of starch syrup for brewing soy sauce |
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Open date: 20100609 |