CN104473065A

CN104473065A - Hibiscus esculentus-fungus-algae composition nutrient and preparation method thereof

Info

Publication number: CN104473065A
Application number: CN201410572137.4A
Authority: CN
Inventors: 庄济诚; 李祥麟; 周锦明; 钱丽华; 王岚; 石玉洁
Original assignee: SUZHOU YUANMENG NEW ENERGY ENVIRONMENTAL PROTECTION TECHNOLOGY Co Ltd
Current assignee: SUZHOU YUANMENG NEW ENERGY ENVIRONMENTAL PROTECTION TECHNOLOGY Co Ltd
Priority date: 2014-10-23
Filing date: 2014-10-23
Publication date: 2015-04-01

Abstract

The invention relates to a hibiacus esculengtus-fungus-algae composition nutrient and a preparation method thereof. The hibiscus esculentus -fungus-algae composition nutrient comprises 30-50 parts of hibiacus esculengtus, 15-35 parts of fungi and 35-50 parts of alga. Through a bioengineering technology, various food active ingredients are organically combined by an enzyme as a main link to form the composition nutrient. The composition nutrient has high beneficial and active substance content and good effects, can be used as a nutrition additive and be added into various foods, can be processed to form foods with special functions by different manufacturing methods (means), improves a sub-health state by daily diet and can prevent or treat or assistantly treat some diseases by food.

Description

A kind of okra, bacterium, algae composition fuel and preparation method thereof

Technical field

The present invention relates to formula and the preparation method of a kind of dietotherapy (function) fuel, particularly relate to a kind of okra, bacterium, algae composition fuel and preparation method thereof.

Background technology

Air, pollution of waterhead is in recent years day by day serious, and gas (elephant) is waited unusual; The change of the temperature difference, sleet is rapid greatly, and impel the variations such as pathogeny bacterium to strengthen, burst disease (SARS, bird flu etc.), makes human body decline to the adaptive capacity of environment; In addition illegal, make and sell of poor quality without good businessman, add poisonous/harmful substances (water-injected meat, waste oil, melamine etc.), people's resist the disease ability (immunity) is impelled to decline, be in sub-health state, accelerate disease (cancer) and development and old and feeble process occur.

For this reason, we are while striving for clean air and water source, and must strengthen the protection of self, except suitable exercise motion, food is absolutely necessary, and the food with food therapy function is one of kind of people's prioritizing selection.

The application of functional food is the problem paid much attention to both at home and abroad at present, its development trend is also huge and far-reaching, along with external culture extensively exchanges, " dietotherapy " of China also imports into all over the world, even some product becomes external patent, " rhubarb wine " as famous in Italy, this drinking utensils has whet the appetite ante cibum, help digestion after meal, the effect of defaecation next day, often drink gut purge stomach, the effect of promoting longevity, the character of this wine enters higher fatty acid with cattle and sheep meat for many masses and occidentals's group shot to NORTHWEST CHINA area, the crowd of the habits and customs such as high protein plays good dietary function." the bucket nurse wine " of " gin " of the U.S., France and " breathing out nurse wine " etc. are all relevant with " dietotherapy " medicine of China, as can be seen here " dietotherapy " generally applied by the attention of the people all over the world.According to incompletely statistics, Singaporean (2006) reaches 6,500 ten thousand dollars for the expense of dietotherapy.

Brainstrust is foretold: the important breakthrough master of health care aspect is from now on if it were not for the new invention from medicine, but from more rational dietary structure and life style, we think that brainstrust said " dietary structure " contains " functional food " with dietary function in fact.

" integration of drinking and medicinal herbs " and " medicine food is with using " is exactly that we know, and as red date, Semen sesami nigrum, white fungus, lotus seeds etc. that we often eat, being ticbit, is again the medicine of curing the disease.Their component contents have the effect regulating human body physiological equilibrium.Change (unbalance) when Human Physiology or suffer just to lose regulating power and ill when infecting (germ invasion).Now as given corresponding functional food, it just can be impelled to recover regulating power, the object reaching diseases prevention and cure the disease, as trouble anemofrigid cold, endure and order ginger Bulbus Allii Fistulosi soup and drink; Aphthae drinks sweet mung bean soup ... like this, too numerous to enumerate.

" dietotherapy " and " food therapy (disease) " is the medical legacy of China's preciousness, enterogastritis is controlled with regard to discovery distiller's yeast as far back as B.C. 579 years, the literature record that diet is cured the disease just is had before the Warring states, go into business thoughtfully bright, the clear epoch, the monograph that special record diet is cured the disease has kind more than 600, effect of sequitur dietotherapy thus.

Summary of the invention

In order to obtain there is the food of food therapy function or be made for the additive of other various functional food, the object of this invention is to provide a kind of okra, bacterium, algae composition fuel and preparation method thereof, solving the demand of integration of drinking and medicinal herbs and tonic.Plant used in this formula, the particular matter all containing needed by human body, the raising of prevention of disease, physique has obvious effect, adjustable human body yin-yang balancing, and nourish comprehensively and improve resistance, delaying senility, is tonic non-defective unit.

Each composition selection gist that the present invention is used and composition, function description:

1, okra

Formal name used at school Hibiacus esculengtus belongs to Malvaceae, annual herb plant.Okra has another name called gumbo, swordweed, plant vigour etc., originate in Africa, Egyptian existing cultivation before more than 2000 years, the ground such as later expanding species (introducing) U.S., Japan, Southeast Asia gradually, Ancient Times in China has the plantation of okra, ground expands and plants and moral education into native breed from China in last century Jiangsu, Shandong, Beijing etc., as: Beijing okra, Beijing Rodococcus sp J-5 etc. nearly more than 20 are planted in various places, as little Song dish-between sword bean-okra (cover) plant, output is a lot, (cover) kind between Shandong Jiang Yuan green onion and okra.

The fruit pod of okra, seed, tender leaf all can be used as vegetables and eat; Especially the stick-slip liquid containing compositions such as water-soluble fibre pectin, galactan, gum arabics in tender fruit pod, the fragrance that tool is special.Also containing the abundant element such as vitamin A, Vb, Vc and Fe, K, P, Zn, Cu, its protein content reaches 15% ~ 26%, carbohydrate is 6.4%, the every 100 grams of heats that can provide 150 kilojoules of fat 2%, cellulose 1%, and concrete composition is as following table 1

Tender okra pod nutrient component meter (every 100 grams of content) table 1

Components Name	Content	Components Name	Content	Components Name	Content
						Edible portion	88	Moisture (gram)	86.2	Energy (kilocalorie)	37
Energy (kilojoule)	155	Protein (gram)	2	Fat (gram)	0.1
						Carbohydrate (gram)	11	Dietary fiber (gram)	3.9	Cholesterol (milligram)	0
Ash (gram)	0.7	Vitamin A (milligram)	52	Carrotene (milligram)	310
						Retinol (milligram)	0	Thiamine (microgram)	0.05	Riboflavin (milligram)	0.09
Niacin (milligram)	1	Vitamin C (milligram)	4	Vitamin (T) (milligram)	1.03
						a-E	0.92	(β-γ)-E	0.11	δ-E	0
Calcium (milligram)	45	Phosphorus (milligram)	65	Potassium (milligram)	95
						Sodium (milligram)	3.9	Magnesium (milligram)	29	Iron (milligram)	0.1
Zinc (milligram)	0.23	Selenium (milligram)	0.51	Copper (milligram)	0.07
						Manganese (milligram)	0.28	Iodine (milligram)	0

Wherein the liquid of special (very) stick-slip obviously can strengthen the energy of human body, disperses fatigue, more can effectively stimulate male sex's nervous centralis, accelerate blood circulation, enhance metabolism, systemic organs's microcirculatory vascular activated, especially with corpora cavernosa penis ...; The mucus being mixed with other composition in addition can also help digest, treatment gastritis, gastric ulcer, heart disease, apoplexy, osteoporosis and senile dementia (Alzheimer disease) disease.Skin (especially with face) is also had to the effect of anti-wrinkle, smooth exquisiteness.

2, fungi plant

2.1, Cordyceps militaris

The composition function of Cordyceps militaris, the national patent got permission according to us:

A, Li Xianglin etc. " antisenescence medicinal composition and method for making thereof ", patent No. ZL02121396.8 international Patent classificating number: A61K35/78;

B, Li Xianglin etc. " improve phycomycete food of athlete's physique and preparation method thereof ", patent No. ZL03153155.5 international Patent classificating number: A23L1/28;

C, Yang Jie, Li Xianglin etc. " utilize Cordyceps militaris cocoon to prepare the method for health-care edible capsule ", the patent No. 201210394933.4;

D, Yang Jie, Li Xianglin etc. " prepare the method for the business alcoholic drink mixed with fruit juice of health food (treatment) " with Cordyceps militaris cocoon extract, patent No. 201210394729.X;

E, Yang Jie, Li Xianglin etc. " prepare the fuel beverage of health food and the method for electuary with Cordyceps militaris cocoon extract ", patent No. 201210393512.X;

Its composition function all illustrates in above-listed patent, repeats no more herein.

2.2, Hericium erinaceus

Hericium erinaceus protein content is higher, every hectogram is containing 26.3 grams, protein, containing more much higher sugar, minerals and vitamins, 18 seed amino acids (wherein having 8 kinds to be that human body is necessary) bran histidine content high especially (0.64mg/g), Hericium erinaceus, containing unrighted acid, can reduce cholesterolemia and content of triglyceride, adjusting blood lipid, being beneficial to blood circulation, is the ultimate food of cardiovascular patient; Polysaccharose substance isoreactivity material suppresses helicobacter pylori (cancer of the stomach), has raising body immunity, delays senility; Fatty 4.2 grams of every hectogram hedgehog hydnum, is genuine high protein, low-fat food, is also rich in various vitamin and inorganic salts in addition.Hericium erinaceus improves a poor appetite, and strengthens barrier of gastric mucosa function, improves lymphocyte transformation rate, promotes the effects such as leucocyte.Therefore the immunocompetence of human body raising to disease can be made; Containing several amino acids and abundant polysaccharide body in Hericium erinaceus, can be aid digestion, to illnesss such as the disease of digestive tracts such as gastritis, cancer of the stomach, cancer of the esophagus, gastric ulcer, duodenal ulcer, superficial gastritis, neurasthenia, dizzy, impotence, there is curative effect, and have booster action to treatment intestinal cancer; Elderly and infirm eats Hericium erinaceus, has the effect of nourishing and fit keeping function; The polysaccharide body that it contains, polypeptide class and fatty material, the synthesis of inhereditary material in energy inhibition cancer cell, thus prevention and therapy alimentary tract cancer and other malignant tumours; Hedgehog hydnum or good nourishing food, have good efficacy to neurasthenia, digestive tract ulcer.In screening anticancer medicine, find that it has obvious anticancer function to skin, muscle cancerous swelling.So often eat Hericium erinaceus, anosisly can strengthen resistance against diseases, ill can the effect of disease therapy; Carry according to " Chinese medicinal fungi ", Hericium erinaceus taste is sweet, property puts down, can sharp the five internal organs, aid digestion, nourishing, to disease of digestive tract--gastric and duodenal ulcer, neurasthenia has good curative effect.Numerous food and medicine are made at present.The traditional Chinese medical science is thought, the flat taste of Hericium erinaceus property is sweet, and favourable the five internal organs, aid digestion, the effect such as nourish the body.Since 20 century 70s, modern medicine proves that Hericium erinaceus has good medical value successively, and clinical practice shows, Hericium erinaceus can treat the diseases such as indigestion, gastric ulcer, antral gastritis, stomachache, gasteremphraxis and neurasthenia.And slight neurastheniac, edible Hericium erinaceus be can yet be regarded as good supplemental treatment.On clinical practice, Hericium erinaceus is adopted to treat, for slight or severe neurological is weak all can obtain comparatively significantly desirable curative effect.

2.3, Poria cocos

Containing pachymic acid, β-pachyman, triterpene compound ethene pachymic acid and natural gum, chitin, protein, fat, lecithin, glucose, adenine, histidine, choline, β-pachyman catabolic enzyme, lipase, protease etc., contained pachymic acid has develop immunitypty, antitumor and calm, hypoglycemic etc. effect; Main component in Poria cocos is pachyman, and content is very high.Inhibitory action is had to various bacteria; Can hydrochloric acid in gastric juice be fallen, have preventive effect to digestive tract ulcer; Significant protective effect is had to hepatic injury; There is antineoplastic action; Many-sided immunologic function can be regulated; Neuroleptic Leukocytopenia caused by chemotherapy can be made to accelerate; And have calm effect; The functions such as cure mainly eliminating dampness and diuresis, beneficial spleen and stomach, antitoxic heart-soothing and sedative, control urine and pay no attention to, oedema is swollen (expiring), and phlematic vomiting, has loose bowels, seminal emission, stranguria with turbid discharge, and palpitation with fear is forgetful, hypoglycemic; Anticancer, belong in the insufficiency of the spleen with overabundance of dampness, phlegm and retained fluid in the cancer knurls such as the clinical conventional treatment cancer of the esophagus, cancer of the stomach, liver cancer, nasopharyngeal carcinoma, tongue cancer, breast cancer, carcinoma of urinary bladder, lung cancer, exedens melanoma stop, damp and hot heap soil or fertilizer over and around the roots knot person etc.

2.4, glossy ganoderma

Glossy ganoderma includes K, P, Mg, Ca, Na, Fe, Cu, Mn and the multiple element such as germanium, selenium and ganodenic acid nucleotides.Amino acid polypeptide, enzyme, polysaccharide etc., from glossy ganoderma, isolate GL-B kind more than 200 at present, wherein major part is β-dextran, research confirms that this polysaccharide can suppress the generation of free radical, strengthen the phagocytic activity of macrophage, improve body hypoxia-bearing, the activity of enhancement of SOD Superoxide dismutase, oxygen radical is suppressed to produce and erythrocyte lipid peroxidation reaction, the ability of raising liver, marrow, blood synthetic DNA (DNA) and RNA (ribonucleic acid) protein; GL-B is one of principle active component of glossy ganoderma, has antitumor, immunological regulation, hypoglycemic, anti-oxidant, reducing blood lipid and anti-aging effects.Triterpenes contained by glossy ganoderma not lower over one hundred kind, wherein based on tetracyclic triterpenes, the bitter taste of glossy ganoderma is relevant with contained triterpenes, triterpenes is also one of active ingredient of glossy ganoderma, to human liver cancer cell, there is CDCC, also can suppress the release of histamine, there is hepatoprotective effect and there is anti-allergic effects etc.; Belong to traditional valuable ingredient of traditional Chinese medicine, possess very high medical value, the modern pharmacology research through scientific research institution's many decades confirms, glossy ganoderma, for enhancing body immunity, regulates blood sugar, controls blood pressure, adjuvant therapy chemicotherapy, liver protecting, promotes that the aspects such as sleep all have significant curative effect.

3 algae

3.1, the composition function of spirulina (arthrospira), according to the national patent that we are granted:

A, Zeng Zhaoqi, Li Xianglin etc. " joint (spiral shell) revolves algae health-care food composition and method for making thereof ", patent No. CN94112205.0;

B, Zeng Zhaoqi, Li Xianglin etc. " blunt epimerite (spiral shell) revolves algae albumen and the application thereof " patent No.: CN94109295.X;

" its composition function illustrates in above-listed patent, no longer repeats herein for the preparation method of the fuel that relieves the effect of alcohol (beverage), capsule of sobering up, the patent No.: ZL200410090707.2 for C, Li Xianglin etc.

3.2, laver

Its protein, iron, phosphorus, calcium, riboflavin, carrotene equal size occupy the hat of various vegetables, therefore laver has again the laudatory title of " nutrition treasure-house ".Every 100 grams of inner proteins 28.2 grams, 0.2 gram, fat, 48.5 grams, carbohydrate, Ca343mg, p457mg, Fe33.2mg, carrotene 1.23mg, VB11 .8mg, VB22.07mg, niacin 5.1mg, vitamin C 1.0mg, iodine 1.8mg, present research confirms that the content of VA in laver is 67 times of milk, particularly content of iodine is higher, this had not only prevented endemicgoiter greatly but also the cholesterol that can remove on blood in human body in tube wall, prevention heart and brain nephro-angiosclerosis prevention white hair and baldness; Effect that laver also has resolving phlegm and softening hard masses, clearing away heat and promoting diuresis, kidney tonifying are nourished heart; Nutritious, amount of iodine is very high, and can be used for " Thyroid Gland Swell " for the treatment of because iodine deficiency causes, laver has softening and resolving hard mass function, and amassing block to other pent-up also has purposes; Be rich in choline and calcium, iron, memory can be strengthened, treatment woman anemia, the growth promoting bone, tooth and health care; Containing a certain amount of sweet mellow wine, can be used as the accesary foods for the treatment of oedema; Polysaccharide contained by laver has obviously enhancing cellular immunity and humoral immune function, can promote lymphocyte transformation, improves the immunity of body; Significantly can reduce the total content into serum cholesterol; The active ingredient of laver, to the inhibiting rate 53.2% of ehrlich carcinoma, contributes to the control of the tumours such as brain tumor, breast cancer, thyroid cancer, malignant lymphoma.

Trophic analysis: the life active compound of (1) protein and amino acid laver, extremely important for plant vital activity.The general protein content 24%-28% of edible laver, far away higher than general vegetables, and the necessary amino acid content of human body is many.The composition amino acid of purple laver protein is as neutral in alanine, aspartic acid, glutamic acid, glycine, proline etc., acidic amino acid is more, and this is the unexistent features of all Lu Sheng vegetable plants.

(2) fat is compared with terrestrial plant, and the fat content of laver is low, and how below 1%, in the fat of laver, the palmitic acid of saturated fatty acid accounts for the overwhelming majority, is about 20%; The acid of Pork and beans green onion, stearic acid content is less, accounts for 2% respectively; Having the unsaturated fat-linoleic acid of fine health-care effect, leukotrienes or stearidonic acid content more to human body, is 10%---30%, and the highly unsaturated fatty acid of more than C20 also has certain content.More optimizedly likened to the Content of Eicosapentaenoic Acid of " docosapentaenoic acid " up to 30% by people in laver.

(3) vitamin laver contains multivitamin, and the content of B family vitamin is not a halfpenny the worse compared with vegetables.In laver, particularly in terrestrial plant, almost the content of non-existent vitamin B12 is very high for B family vitamin, and in dry, the content of vitamin B12 is close with the flesh of fish, and ascorbic content is also very high.Vitamin B12 enlivens cranial nerve, anti-aging and be losing one's memory in advance, improves effect of melancholia.

(4) inorganic composition that inanimate matter is very abundant because seawater contains, and laver has and absorbs and the function of inanimate matter in savings seawater, because the inanimate matter content in a little laver is very abundant.

3.3, sea-tangle

Sea-tangle contains vitamin B12 0.7ug%, B2810mg% carrotene 0.77% such as the multiple necessary protein of human body, amino acid, proline, isoleucine, alanine, glycine, water-soluble potassium chloride, iodine, calcium, bromine, phycocolloid acid, laminaran, sweet mellow wine, inorganic salts, organic acid, leukotrienes methyl alcohol, pohytol, palmitic acid, fucosterol, sky bridle terpene, inner wheat straw lactones, polysaccharide etc., pharmacology: 1, can correct thyroid function deficiency; 2, the absorption of pathological product (inflammation) exudate can be promoted; 3, hypotensive: laminine and sylvite, calcium constituent can reduce the absorption of human body to cholesterol, reduce blood pressure; 4, antiasthmatic effect; 5, replenish the calcium: sea-tangle contains abundant calcium, human body calcium deficiency can be prevented and treated; 6, improve looks: kelp juice can beautifying face and moistering lotion, and the soup juice be boiled into sea-tangle is bathed, and skin of can moistening, makes skin refreshing fine and smooth, bright and clean beauty; 7, lose weight: sea-tangle contains a large amount of dietary fibers, can increase the satiety of overweight people, and sea-tangle fat content is very low, and heat is little, is the food that overweight people loses weight; 8, delay senility: kelp Rich contains selenium, can effective scavenging free radicals, and the arch-criminal of free radical human senility just, so the effect that sea-tangle can delay senility.9, inhibition cancer cell; The strontium of the sodium alginate contained by sea-tangle and tool carcinogenesis, cadmium have very strong binding ability, and they are excreted; Sea-tangle alternative is killed or is suppressed can produce carcinogenic bacterium in enteron aisle, and contained fiber can also promote the discharge of bile acid and cholesterol; Thallus Laminariae (Thallus Eckloniae) extract has direct repression to various cancer cell; 10, radioresistance: the extract laminarin of sea-tangle has radiation resistance because of Immunosuppression Apoptosis; Modern medicine study confirms that its extract matrium alginicum has anastalsis, and pre-preventing leukemia, Itai-itai diseases, putting property of minimizing element are by intestinal absorption.

The technical solution used in the present invention is as follows:

A formula for okra, bacterium, algae composition fuel, with its component of weight parts is:

Okra 30-50 part, bacterium 15-35 part, algae 35-50 part;

The okra that the present invention selects adopts the okra of green organic plantation;

Bacterium that the present invention selects adopts any one or the combination of Cordyceps militaris, Hericium erinaceus, Poria cocos, glossy ganoderma;

Algae that the present invention selects adopts any one or the combination of spirulina (spirulina maxim or blunt top spirulina), laver, sea-tangle.

The present invention also disclosed a kind of method being prepared into fuel with above-mentioned formula, comprises the steps:

1) take okra 30-50 part, bacterium 15-35 part, algae 35-50 part, each raw material is pulverized, and (crossing 100 mesh sieves) is mixed to get compound;

2) get step 1 compound 50g, add 10 times of water, adjust pH value to 6.8-7.5 with NaHCO3, add neutral proteinase 0.2-0.3g, liquid temperature keeps 30-40 DEG C, effect 8-10 hour;

3) in the solution that step 2 obtains, add monascus and each 0.20-0.25g of amylase, adjust pH value 2-3 with HCL, be heated to 80 degree, collect precipitate, add 20-25% water, use 40%NaOH solution, adjust pH value to 7;

4) solution of step 3 is added equivalent 95% ethanol, stir evenly, adding HCL again adjusts pH value 2-3 to stir evenly, heating makes its inclusion separate out, filter, add in residue 50% ethanol stir refilter, merging filtrate vacuum microwave drying fuel, ethanol before it is dried or can be reclaimed in drying to utilize.

Advantage of the present invention is: applied bioengineering technology, with enzyme for main tie, the active ingredient of various food is organically combined and obtains fuel, this fuel makes the food of the function that differs from one another by different production methods (means), improve sub-health state by diet, prevent or treated by food or some disease of supplemental treatment.Formula of the present invention is simple and easy to get, and preparation technology's science, advanced person, can be mass, and manufactured goods active ingredient is high, both can be used as dietotherapy product and also can be used as invigorant and take, and has treatment and prophylactic function.

Accompanying drawing explanation

Accompanying drawing is preparation technology's flow chart of the present invention.

Below in conjunction with embodiment, further describing is done to the present invention.

Detailed description of the invention

Embodiment, with reference to accompanying drawing: the formula of a kind of okra, bacterium, algae composition fuel, with its component of weight parts is:

Okra 30-50 part, bacterium 15-35 part, algae 35-50 part;

The okra that the present embodiment is selected adopts the fresh okra of green organic plantation;

The bacterium that the present embodiment is selected is Poria cocos, also can adopt any one or the combination of Cordyceps militaris, Hericium erinaceus, glossy ganoderma;

The algae that the present embodiment is selected is spirulina maxim, also can adopt any one or the combination of blunt top spirulina, laver, sea-tangle.

The present embodiment also disclosed a kind of method being prepared into fuel with above-mentioned formula, comprises the steps:

The traditional Chinese medical science, when dialectical treating, pays much attention to consumption.To different diseases, patient that the even same disease of different patients is different, though use same medicine, its quantity is different, even has a long way to go; Quantity is crucial, and this is also that we explore the result obtained in the research functional food of tens of years.

The relevant research of the fuel that the present invention obtains and experiment: complete the clinical observation carrying out zoopery and volunteer under acute toxicity testing " 50LD " and Ames test the prerequisite had no side effect.Every test result is as follows:

1. method

" fuel " that extract, by putting into practice the quantity, proportioning example, technique etc. grasped, mixes and makes various forms of and have the food of function.

Zoopery

(1) antifatigue experiment

A: swimming test: the small white mouse 30 of getting body weight 20-25 gram, be divided into 2 groups at random, first group is fed general food (comparing): second group is fed containing 5% fuel (food, beverage), twice daily, every mouse feeding product 2 grams at every turn, 2 groups of small white mouses, after continuous 20 days, are dropped in water vat by beverage 3ML simultaneously respectively, their swimming time of observed and recorded, result is as shown in the table:

Small white mouse antifatigue contrast test table 2

To feed number of days	First group (average minute)	Second group (average minute)
			1	8.2	8.0
3	8.4	8.3
			5	8.1	8.5
7	8.0	8.8
			9	8.0	8.7
15	8.2	9.4
			20	8.2	10.6

As seen from Table 2, feed in 1-3 days, the swimming time of 2 groups of mouse is close, without much differences, during by the 7th day, many than control group 0.5 minute of the swimming time of the second group mouse of hello fuel (food, beverage), extends (increase) 2.4 minutes to when 20 days.The trend of their time expand strengthens gradually simultaneously, relatively steadily.This shows that fuel (food, beverage) has the effect of delay fatigue.

B: pole-climbing (ability) is tested: the first and second 2 groups of mouse are placed in rotary speed 16 revs/min, on the wooden round bar of diameter 2.5CM, observe the time that they creep on bar, result is as follows:

Small white mouse ability contrast test table 3

To feed number of days	First group (average minute)	Second group (average minute)
			1	28	25
3	29	28
			5	30	31
7	32	35
			9	33	38
11	44	54
			15	43	58
20	44	64

From upper table, feed at the beginning of beginning, 2 groups of Endurances are close, and from the 7th day, the pole-climbing ability of second group mouse was strengthened gradually, and organize prolongation 20 minutes to contrasting to when the 20th day, this shows that fuel can make the endurance of mouse strengthen.Meanwhile, zoopery is also pointed out, and fuel food, beverage need continuous longer-term to take, and short-term drinks DeGrain.

C: high-low temperature resistant is tested: get male 6 weeks healthy mices 30, every body weight 21 ± 2g, is divided into 2 groups at random, often organizing 15 is one group, feeds every day and is respectively:

1 group: 5ml/22g (drinking water) body weight of feeding every day+feed 2g (control group);

2 groups: feed every day containing the beverage 5ml/22g body weight+containing the food 2g (fuel group) of 10% fuel of 10% fuel;

Fuel affects table 4 to small white mouse high/low temperature swimming time

*P＜0.05,P＜0.01

Experimental result: fuel group in 43 degree of water went swimming prolonged survival period 199.22% (P < 0.01), in 8 degree of water went swimming time lengthening 73.50% (P < 0.01).This shows that fuel food can significantly improve the time-to-live of mouse passive swimming in 43 degree, 8 degree water and in dose-effect relationship, point out the obvious emergency capability of tool and dynamic endurance.

D, improve the effect of body functions of intestines and stomach: get male and healthy small white mouse 30, be divided into 2 groups at random, often organize 15, every day, feeding concentration was respectively:

1 group: physiological saline 0.5ML/21g+ normal diet (control group) of feeding every day

2 groups: feed every day with the food containing fuel 15% of amount; Feed continuously 8 days, fasting 24 hours in 9th day, within 10th day, to gavage after above-listed various beverage/food 25 minutes, gavage black carbon meal (5% active carbon again, 1.2% agar) 0.5ML/21g body weight, after 15 minutes, cervical dislocation puts to death mouse, careful taking-up Mouse Stomach orifice of the stomach, to rectum end one section of alimentary canal, without on tractive Horizon flooring, measure the distance (A) of this section of alimentary canal total length and the orifice of the stomach distance (B) to carbon dust end, statistics often organizes the percentage=B/A*100% of mouse carbon dust in alimentary canal movement, and result is as shown in the table:

Fuel affects table 5 to enterogastric peristalsis

Group	Carbon meal mobility (%)	Increase percentage (%)
			Physiological saline group	35.38±3.86	-------
Fuel group	59.84±4.47	69.13

Experimental result shows: fuel food obviously promotes enterogastric peristalsis, and carbon eats mobile increment rate up to 69.12%, P < 0.05, significant difference, and the obvious dose-effect relationship of tool.

E, immunization experiment: burn mouse leg belly with alcolhol burner, the gross area about 35%, the degree of depth is 5% II+wound, be fed with the functional food 4 of 20% fuel every day, every 0.25g and the beverage 6ml containing 20% fuel, and with beverage by functional food furnishing pasty state, be coated with abrasion, observe wound healing situation and detect hemolysin, the items such as lymphocyte transformation.Result: the mouse wound healing of feeding function and beverage is fast, and hemolysin, lymphocyte etc. are all improved.

F, the SOD value detected in blood: get (14 months) of the same age, body weight is the mouse totally 20 of 25-28 gram, be divided into 2 groups at random, A group is fed normal food, water, B group is fed containing the food of fuel 25% and beverage, gets their blood after continuous 90 days, detect wherein SOD active, in result B group blood, SOD specific activity A group on average increases by 8.4 active units.

G, the experiment of enhancing memory: A group (feeding normal food) B group (feeding above-mentioned containing fuel food) mouse is passed through the overhead lost experiment of cross, the experiment of shuttle box behavior is carried out system and is observed rear surface, and the memory of B group mouse is significantly improved than A group.

Experiment proves, the fuel made with okra, algae and mushroom medicine-food two-purpose food, has and significantly suppresses the generation of MDA and the effect of MAOA, can increase the activity of SOD, improves immunity and increases erythrocyte number; Promote gastrointestinal peristalsis; Improve resist oxygen lack power, strengthen muscular strength and endurance; Added body (power) energy and body fluid, strengthen the repair of wound.

2. practice/application test

Obtain on obvious basis at animal experiment, first through Jiangsu, Shanghai, 50 volunteers in ground such as Anhui interruption take on probation, their fitness has improvement in various degree, raising.Especially with sportsman fierceness (training) match in, under the state of fever, in metabolism accelerator, produce a kind of gene-free radical having strong oxidation.When intracellular free radicals exceedes some, human body is had an impact and damages.It makes cell membrane sex change, rotten, and hereditary information is made a mistake, and makes protein lose due biological function (fatigue) etc. and destroys the rule (in body lactic acid gathering etc.) of metabolism.

A, tired aching pain of muscles recover (disappearances) and test: each for men and women 10 sportsmen are divided at random experimental group-add containing 30% fuel beverage, control group-disobedience fuel, observe, compare them and train rear fatigue (aching pain of muscles) recover the degree of (disappearances) and athleticly to feel, employing accumulation scoring method.

Accumulate the standard of scoring:

A, after 24 hours, fatigue disappears completely, and muscle does not have obvious ache-5 point

B, after 24 hours, fatigue is recovered substantially, and ache alleviates a lot-4 points

C, after 24 hours, still some tired and ache, but not serious-3 point

D, after 24 hours, still feel tired, aching pain of muscles slightly extenuates-2 points

E, after 24 hours, tired, aching pain of muscles does not disappear, and maintains the original state-1 point

After observing, comparing 14 days, result is as following table: table 6

Improvement rate	﹥30％	﹥50％	﹥60％
				Fuel group (%)	38	22	88
Control group (%)	33	13	51

From upper table, disappearance tired after taking fuel and aching pain of muscles extenuate degree, all comparatively refuse to obey fuel obvious-improvement rate is higher.

The change of SOD content in B, blood: try clothes through 16 volunteers, every day three times, each beverage 20ML containing fuel 30%, containing the nutraceutical 50 grams of fuel 30%, after continuous 3 months, detecting their SOD content, being increased to 34.68 units by taking 18.84 units before product; The formal name used at school of SOD is superoxide dismutase, and it a kind ofly comes from the active material of life entity and anti-ageing enzyme, can the Cell protection not infringement of " free radical " in acceptor.When it and free radical meets time, impel free radical to be decomposed into harmless hydrone and oxygen molecule.According to Long-Term Scientific Study, find that the physiological function of SOD mainly contains: improve body immunity, delay senility; Effectively reduce that blood is glutinous, blood pressure and blood sugar, have obvious restitution to diabetes, have expert to claim: the content of the SOD in human body was directly proportional to the life-span of its people, SOD has become the health care gold of 21 century.

C, erythrocytic change: red blood cell be blood mainly contain formation/mono-, be made up of hemoglobin and water, its average life span is 90-120 days.Red blood cell is dead or after destroying, hemoglobin is engulfed by the places such as liver, spleen, marrow and is decomposed into globulin and iron content ferroheme.Iron is separated out by iron content ferroheme, becomes bilirubin, makes blood plasma be yellow.

Erythrocyte number: normal male is 400-500 ten thousand/MM

Normal female is 350--500 ten thousand/MM

Be engaged in intense physical activity or excited time, in body, red blood cell number there will be the raising of transience.Hemoglobin forms erythrocytic main component, and erythrocytic main Physiological Function is completed by hemoglobin.Taking continuously as above behind functional food 50 grams and fuel 150ML45 days through 14 volunteers (8 male 6 female), there is following change in their erythrocyte number:

Erythrocytic number change contrast table 7

Sex	Drink-service proerythrocyte (mean value)	Red blood cell (mean value) after drink-service
			Man	450	530
Female	435	520

Hemoglobin is fully combined with oxygen, forms oxygenated haemoglobin.This kind of hemoglobin is mainly in arterial blood.When arterial blood circulation is in histoorgan, hemoglobin and oxygen separating, histoorgan cell obtains oxygen and carries out metabolism.At this moment hemoglobin becomes reduced hemoglobin, carries CO2 and gets rid of external with breathing.Hemoglobin can also play cushioning effect to blood pH, keeps stablizing of human internal environment.Therefore, prompting fuel food has promotes erythrocytic effect, all has positive meaning to gas exchanges acid-base balance in body etc.

The physical demands of sportsman when training (containing match) has certain boundary, and this boundary is again by the restriction of two kinds of factors: one is the reduction of blood sugar concentration; Two is because perspire, the loss of body fluid and cause electrolytical unbalance.This is certainly relevant with environment temperature with muscle power (training) intensity of consumption again.But blood sugar concentration can be made during carbohydrate supplementation to raise, and insulin level improves, and makes the storage direction of glycogen adjust again, but the liquid supplemented is unabsorbable in stomach, can only by could being absorbed in pylorus to intestines.Stomach (pylorus) is normally closed, and its opening and closing is subject to the domination of food (instinct) in stomach.Generally in stomach, food osmotic pressure is lower, and the speed to bowel movement is faster, and water and the carbohydrate speed from stomach to intestines is the principal element of restriction supplemental amount.

Fuel is nutritious equilibrium not only, but also the inorganic salt and other material because of a large amount of loss of perspiring can be supplemented containing okra, algae and mushroom polysaccharide etc. (this kind of material is better than monosaccharide-glucose, fructose etc.) and appropriate inorganic salts and trace element, vitamin etc. uniquely, its osmotic pressure is lower simultaneously, intestines can be entered quickly through stomach, absorbed as soon as possible, thus supplemented physical consumption.

Experiment shows, sportsman is in " during geo-stationary (not training and competition) state; (average content is 4.2 ± 3mg/L to the blood sugar namely before motion, and from (activity) training, blood sugar just rises gradually; rise to 22 ± 2mg/L to blood sugar when 45 minutes; reach peak, gradually decline later, until at the end of (activity) training; blood sugar still remains between 9--11mg/L, all the time higher than the level before (activity) training.Can return to through (geo-stationary) level originally (not having activity, during training) after 2 hours again, its result is as shown in the table:

The change of blood sugar contrast table 8 of the motion person of seesawing

The present invention is applied bioengineering technology, with enzyme for main tie, the active ingredient of the various foods in formula is organically combined the fuel obtained, effective benefit materials content is high and make good use of, and be easily added in varieties of food items and make nourishing additive agent, made the food of the function that differs from one another by different production methods (means), improve sub-health state by diet, prevent or treated by food or some disease of supplemental treatment.The present invention's formula is simple and easy to get, and preparation technology's science, advanced person, can be mass, and manufactured goods active ingredient is high, namely can be used as dietotherapy product and also can be used as invigorant and take, can treat and prophylactic function.

Claims

1. a formula for okra, bacterium, algae composition fuel, with its component of weight parts is:

Okra 30-50 part,

Bacterium 15-35 part,

Algae 35-50 part;

Described okra adopts the okra of green organic plantation;

Described bacterium adopts any one or the combination of Cordyceps militaris, Hericium erinaceus, Poria cocos, glossy ganoderma;

Described algae adopts any one or the combination of spirulina (spirulina maxim or blunt top spirulina), laver, sea-tangle.

2. formula according to claim 1 is prepared into a method for fuel, comprises the steps:

1) each raw material taken in power 1 is pulverized, and (crossing 100 mesh sieves) is mixed to get compound;

3) in the solution that step 2 obtains, add monascus and each 0.20-0.25g of amylase, adjust pH value to 2-3 with HCL, be heated to 80 degree, collect precipitate, add 20-25% water, use 40%NaOH solution, adjust pH value to 7;

4) solution of step 3 is added equivalent 95% ethanol, stir evenly, then add HCL and adjust pH value 2-3 to stir evenly, heating makes its inclusion separate out, filters, and adds 50% ethanol stirring and refilter in residue, merging filtrate vacuum microwave drying fuel.