CN104459100B - 一种抗sm-rnp抗体试剂盒 - Google Patents

一种抗sm-rnp抗体试剂盒 Download PDF

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CN104459100B
CN104459100B CN201410685351.0A CN201410685351A CN104459100B CN 104459100 B CN104459100 B CN 104459100B CN 201410685351 A CN201410685351 A CN 201410685351A CN 104459100 B CN104459100 B CN 104459100B
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positive control
serum
control serum
rnp antibody
rnp
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CN104459100A (zh
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张志新
叶正义
何远立
杨秦
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CHENGDU VERO BIOTECHNOLOGY Co Ltd
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    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
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Abstract

本发明公开了一种抗SM-RNP抗体试剂盒，所述试剂盒包括用于表达抗SM-RNP抗体的细胞载体、阴性对照血清、阳性对照血清、酶底物、浓缩样本稀释液，所述阳性对照血清为病人的阳性对照血清筛选后的对照血清。本发明通过对所采集的阳性对照血清进行筛选，取分布比较密集的数值区域的阳性对照血清作为抗SM-RNP抗体试剂盒中的阳性对照血清，克服了现有试剂盒在检测人血清中抗SM-RNP抗体的含量时，因每个批次的阳性对照抗体不稳定而导致结果不稳定的问题。

Description

一种抗SM-RNP抗体试剂盒
技术领域
本发明涉及试剂盒领域，具体地，涉及一种抗SM-RNP抗体试剂盒。
背景技术
系统性红斑狼疮简称SLE是一种以大量自身抗体形成为特征，导致多系统、多器官损害的慢性系统性自身免疫疾病。系统性红斑狼疮是一种常见的风湿性疾病，经调查显示，该病的发病率为千分之一左右，好发于育龄女性，男女比列约为1:7～10，非生育年龄女性优势不明显。主要病因可能与遗传、环境因素和雌激素有关系，已有研究报道年龄是一大危险因素，尤其是青年女性发病最为常见。发病机制主要与免疫调节异常、大量的自身抗体以及免疫复合物的形成有关系，目前，具体的发病机制尚不清楚。
SLE血清检测是SLE普查和辅助诊断的重要方法。目前，医院实验室主要采用酶联免疫吸附实验及ELISA法对患者的血清进行初步的诊断，但是目前的诊断方法都是需要阳性对照液，而阳性对照液的来源一般都是来自病人的血清。目前，在检测人血清中抗SM-RNP抗体的检测试剂盒中，阳性对照抗体多数都来自病人的阳性对照血清，这样，每个批次的试剂盒，阳性对照或许不同，导致每个批次中阳性对照抗体不稳定的问题。
发明内容
本发明所要解决的技术问题是提供一种抗SM-RNP抗体试剂盒，以克服现有试剂盒在检测人血清中抗SM-RNP抗体的含量时，因每个批次的阳性对照抗体不稳定而导致结果不稳定的问题。
本发明解决上述问题所采用的技术方案是：一种抗SM-RNP抗体试剂盒，其特征在于，所述试剂盒包括用于表达抗SM-RNP抗体的细胞载体、阴性对照血清、阳性对照血清、酶底物、浓缩样本稀释液，所述阳性对照血清为病人的阳性对照血清筛选后的对照血清。
现有的抗SM-RNP抗体试剂盒中的阳性对照血清来自病人的阳性对照血清，这样，每个批次的试剂盒，阳性对照或许不同，导致每个批次中阳性对照抗体不稳定的问题，本发明通过对所采集的阳性对照血清进行筛选，取分布比较密集的数值区域的阳性对照血清作为抗SM-RNP抗体试剂盒中的阳性对照血清，克服了现有试剂盒在检测人血清中抗SM-RNP抗体的含量时，因每个批次的阳性对照抗体不稳定而导致结果不稳定的问题。
进一步地，细胞载体为哺乳动物细胞。哺乳动物细胞与人体细胞环境具有较高的相似度，且哺乳动物细胞比价容易获得，能够降低实验成本。
一种抗SM-RNP抗体试剂盒的制备方法，包括以下步骤：
首先，细胞载体的制备：将表达有抗SM-RNP抗体的细胞载体活化后，在4℃培育5小时，用PBS缓冲液洗涤、离心、弃上清液，加入一定浓度的血清蛋白封闭液，培育30分钟，完成细胞载体的制备；
其次，其它组分的制备：阴性对照血清是健康人的血清，阳性对照血清为已经确诊的抗SM-RNP抗体阳性血清，浓缩样本稀释液中含有PBS、哺乳动物血清蛋白。
综上，本发明的有益效果是：
1、本发明通过对所采集的阳性对照血清进行筛选，取分布比较密集的数值区域的阳性对照血清作为抗SM-RNP抗体试剂盒中的阳性对照血清，克服了现有试剂盒在检测人血清中抗SM-RNP抗体的含量时，因每个批次的阳性对照抗体不稳定而导致结果不稳定的问题。
2、本发明通过将载体设置为哺乳动物细胞，哺乳动物细胞与人体细胞环境具有较高的相似度，且哺乳动物细胞比价容易获得，能够降低实验成本。
具体实施方式
下面结合实施例，对发明作进一步地详细说明，但本发明的实施方式不限于此。
实施例：
一种抗SM-RNP抗体试剂盒，试剂盒包括用于表达抗SM-RNP抗体的细胞载体、阴性对照血清、阳性对照血清、酶底物、浓缩样本稀释液，所述阳性对照血清为病人的阳性对照血清筛选后的对照血清。细胞载体为哺乳动物细胞。
一种抗SM-RNP抗体试剂盒的制备方法，包括以下步骤：
首先，细胞载体的制备：将表达有抗SM-RNP抗体的细胞载体活化后，在4℃培育5小时，用PBS缓冲液洗涤、离心、弃上清液，加入一定浓度的血清蛋白封闭液，培育30分钟，完成细胞载体的制备；
其次，其它组分的制备：阴性对照血清是健康人的血清，阳性对照血清为已经确诊的抗SM-RNP抗体阳性血清，浓缩样本稀释液中含有PBS、哺乳动物血清蛋白。
如上所述，可较好的实现本发明。

Claims (3)

1.一种抗SM-RNP抗体试剂盒，其特征在于，所述试剂盒包括用于表达抗SM-RNP抗体的细胞载体、阴性对照血清、阳性对照血清、酶底物、浓缩样本稀释液，所述阳性对照血清为病人的阳性对照血清筛选后的对照血清。
2.根据权利要求1所述的一种抗SM-RNP抗体试剂盒，其特征在于，所述细胞载体为哺乳动物细胞。
3.一种抗SM-RNP抗体试剂盒的制备方法，其特征在于，包括以下步骤：
首先，细胞载体的制备：将表达有抗SM-RNP抗体的细胞载体活化后，在4℃培育5小时，用PBS缓冲液洗涤、离心、弃上清液，加入一定浓度的血清蛋白封闭液，培育30分钟，完成细胞载体的制备；
其次，其它组分的制备：阴性对照血清是健康人的血清，阳性对照血清为已经确诊的抗SM-RNP抗体阳性血清，浓缩样本稀释液中含有PBS、哺乳动物血清蛋白。
CN201410685351.0A 2014-11-25 2014-11-25 一种抗sm-rnp抗体试剂盒 Expired - Fee Related CN104459100B (zh)

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JPH0875745A (ja) * 1994-07-06 1996-03-22 Igaku Seibutsugaku Kenkyusho:Kk 可溶性Ｆａｓ抗原の免疫学的測定方法及びその測定用キット
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CN1588076A (zh) * 2004-09-28 2005-03-02 浙江大学 猪ⅱ型圆环病毒抗体间接elisa诊断试剂盒
JPWO2008001944A1 (ja) * 2006-06-29 2009-12-03 独立行政法人理化学研究所 自己抗体検出用試薬及び自己免疫疾患の診断キット
CN101329341B (zh) * 2008-07-09 2012-06-27 北京美康生物技术研究中心 检测自身免疫疾病相关抗核抗体谱的试剂盒及其制备方法
CN201293789Y (zh) * 2008-10-14 2009-08-19 上海裕隆生物科技有限公司 一种红斑狼疮检测蛋白芯片及其试剂盒
CN102360016A (zh) * 2011-07-21 2012-02-22 南京工业大学 流式微球法对SLE的Sm-RNP抗体检测试剂盒的制备
CN102937648B (zh) * 2012-11-14 2014-08-20 四川新健康成生物股份有限公司 一种检测自身免疫疾病相关抗核抗体谱的试剂盒
CN203232035U (zh) * 2013-01-23 2013-10-09 深圳市亚辉龙生物科技有限公司 检测自身免疫性疾病抗体的免疫印迹试剂盒

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