CN104419699A - Biological product containing aerobe combined bacteria and preparation method and application thereof - Google Patents
Biological product containing aerobe combined bacteria and preparation method and application thereof Download PDFInfo
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- CN104419699A CN104419699A CN201310369807.8A CN201310369807A CN104419699A CN 104419699 A CN104419699 A CN 104419699A CN 201310369807 A CN201310369807 A CN 201310369807A CN 104419699 A CN104419699 A CN 104419699A
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Abstract
The invention discloses a biological product containing aerobe combined bacteria and a preparation method and application thereof. The biological product includes aerobe combined bacteria loaded on a porous carrier; and the aerobe combined bacteria includes bacillus and pseudomonas putida. The preparation method includes the steps of: 1) soaking the porous carrier with acid or alkali and rinsing the porous carrier with water to a neutral state to obtain pretreated porous carrier; and 2) loading the aerobe combined bacteria liquid on the porous carrier. The biological product can be used in sewage treatment, and significantly improve the efficiency of biochemical treatment, efficiently remove COD and ammonia nitrogen, improve the outlet water quality and reduced the sludge yield by more than 80%.
Description
Technical field
The present invention relates to a kind of biological products and method for making thereof and application, particularly relate to a kind of biological products containing aerobic microbiological combination bacterium and method for making thereof and application.
Background technology
Environmental microorganism is prevalent in occurring in nature, has source wide, and reproduction speed is fast, to features such as environmental compatibility are strong, has been widely used in water treatment field.Many aerobic microbiologicals, under oxygen existence condition, are mineralized by katabolism, anabolism and material, oxidation operation are resolved into CO
2and H
2o, makes water quality be purified, and in the process, obtains C, N, P, S and energy, make self to be multiplied.Wherein, activated sludge process to have found and since application, through the development of last 100 years from British scientist Clark and Cage in 1912, technique becomes better and approaching perfection day by day, become applicating history in the biological treatment of sewage the longest, the most extensively, and develop the most perfect method in theory and practice.But activated sludge process exists many drawbacks, mainly active low, the surplus sludge volume of generation is large and shock resistance is poor.
Obviously, along with the requirement of country to discharge water water quality is more and more higher, traditional activated sludge process has been difficult to the requirement meeting sewage disposal.Extremely urgent to the transformation of activated sludge process.
Screening and research superior microorganism are the focuses that environment in recent years microorganism man and relevant environmental microorganism production firm pay close attention to.Dominant bacteria is the efficient degrading bacteria made by the method for artificial culture and mutagenesis.Its method is found the flora of certain pollutent of degraded, puts in contaminated environment again, make to play a role after enlarged culturing.Also existing efficient degrading bacteria can be made novel high efficiency engineering bacterium by mutagenesis or the technology such as gene recombination or protoplasm somatocyte fusion.
Black Liquor with Efficient Bacteria through acclimatization culture not only has very high activity and very strong capacity of decomposition, and has good specific aim.Such as, can high concentration organic sewage be processed with photosynthetic bacterium, cellulose sewage can be processed with white mould bacterium, high nitrogenous organic sewage can be processed with nitrobacteria, can Phenol-Containing Wastewater Treatment with candida tropicalis, can prussiate etc. be decomposed with sickle mycete and actinomycetes.The achievement in research industrialization in current many laboratories, commercially, some dominant bacterias are as commodity selling.These products are used as the promotor helping traditional active sludge to start fast a bit, some is used as renovation agent when sludge system goes wrong, the auxiliary being used as improving active sludge treatment efficiency had, particularly when existence active sludge in sewage is difficult to the organism of degrading, the effect that adds of these dominant microfloras is very remarkable.These commercial Black Liquor with Efficient Bacteria products are mostly used in combination with active sludge, i.e. adding portion dominant bacteria in traditional active sludge, to improve the activity of mud.But this Adding Way is difficult to maintain the dominant bacteria degree of depth in systems in which, this is because part microbes in use, discharges together with excess sludge from system.Therefore, in order to maintain the concentration of dominant bacteria, must regularly be supplemented, costly required, affect the popularization of dominant bacteria product.
Summary of the invention
The technical problem to be solved in the present invention is to provide a kind of containing the aerobic microbiological combination biological products of bacterium and method for making thereof and application.Biological products of the present invention can be used for sewage aerobic process, and replace the process that active sludge is used for municipal effluent, the application of these goods can prevent the loss of dominant bacteria, and significantly can improve the efficiency of biochemical treatment, improves effluent quality, reduce the output of excess sludge.
For solving the problems of the technologies described above, the biological products containing aerobic microbiological combination bacterium of the present invention, comprising: the aerobic microbiological combination bacterium being carried on porous support; Wherein, aerobic microbiological combination bacterium comprises: genus bacillus (Basillussubtilis) and pseudomonas putida (Pseudomonas putida strain).
Described porous support need have through hole structure, to ensure the gentle circulation in porous support of water.This porous support comprises: inorganic materials or macromolecular material; Wherein, inorganic materials comprises: one or more in gac, porous zeolite, coke, cinder etc.; Macromolecular material is porous plastics; This porous plastics comprises: one or more in urethane foam, polyvinyl chloride foam (PVC porous plastics), polyvinyl alcohol foam plastics (PVA porous plastics), ethylene vinyl acetate foam plastics (eva foam plastics), polymeric amide porous plastics, polyvinyl chloride foam etc.Be preferably urethane foam and cinder.Described porous support is of a size of 1cm
3~ 400cm
3, be preferably 4cm
3~ 100cm
3.The aperture of porous support is generally 0.1mm ~ 10mm, is preferably 0.1mm ~ 5mm.
The kind of described genus bacillus is more than one.Due to sewage disposal faced by handling object very complicated, therefore, in actual use, not high to the purity requirement of bacterium.Described genus bacillus can comprise: one or more in subtilis (Basillussubtilis), bacstearothermophilus (Bacillus stearothermophilus), bacillus pumilus (Bacilluspumilus), bacillus megaterium (Bacillus megaterium), bacillus cereus (Basillus cereus), Bacillus circulans etc., specifically can select according to the actual needs of sewage disposal.
Genus bacillus of the present invention can be obtained by enrichment, separation from the mud of food processing plant.Wherein, better performances be subtilis, therefore, subtilis (as be numbered the mushroom of DF7658, JD32 and 5114 etc., wherein, this is numbered and obtains through different experimentai batches) is preferred genus bacillus.This subtilis has very strong cleaning action to the starch in municipal effluent and protein component.
In addition, in the present invention, when preparing oxygen animalcule combination bacterium, when adding the genus bacillus except subtilis, the existence of these bacillus can not affect the activity of combination bacterium, therefore, the removal to COD in sewage (Chemical Oxygen Demand) and ammonia nitrogen can not be affected.
A pseudomonas of the present invention, by being separated, screening and obtaining after enrichment from insecticide factory's mud.Through DNA analysis, prove pseudomonas putida (Pseudomonas putida strain CDd-9).This strain flora not only has and very strong goes COD ability, and has very strong deammoniation nitrogen and the ability of degrease.After tested, this bacterial strain is at ammonia nitrogen concentration higher than 1000mg/L, and salt concn reaches in the environment of 3wt% (concentration expressed in percentage by weight) and still can survive.Its ability removing ammonia nitrogen is higher than 90%.
The present invention, by above-mentioned flora Fast-propagation in general nutrient solution, makes bacterial concentration reach 10
8individual/more than ml.Then, these bacterium liquid are mixed with composite bacterial solution in proportion.And under aerobic condition, the sewage being placed in preparation is tamed.
In the present invention, in order to ensure the ammonia nitrogen removal effect of these biological products to municipal effluent etc., in combination bacterium, the content of pseudomonas putida should be not less than 50%.As described in the proportioning of genus bacillus and pseudomonas putida be 1: 1 ~ 1: 5, be preferably 1: 1 ~ 1: 2.
The charge capacity of described aerobic microbiological combination bacterium be on the porous support of every cubic centimetre the bacterium liquid of load 0.1ml ~ 0.5ml aerobic microbiological combination bacterium (namely 0.1ml ~ 0.5ml aerobic microbiological combines the bacterium liquid/cm of bacterium
3porous support), the preferentially bacterium liquid of load 0.1ml ~ 0.2ml aerobic microbiological combination bacterium on the porous support of every cubic centimetre.Wherein, aerobic microbiological combines the total cell concentration in the bacterium liquid of bacterium is 10
7individual/more than ml.
In addition, the invention also discloses a kind of preparation method of the biological products containing aerobic microbiological combination bacterium, comprise step:
1) clean (pre-treatment) of porous support
After soaking porous support with acid or alkali, then with water rinse to neutral, obtain pretreated porous support, to remove the material poisonous to microorganism growth that may exist in porous support;
2) the bacterium liquid of above-mentioned aerobic microbiological combination bacterium is carried on through step 1) on the porous support that processes.Wherein, charge capacity can be described above.
Described step 1) in, acid comprises: mineral acid; Wherein, mineral acid comprises: the one in hydrochloric acid, sulfuric acid or its combination, preferred HCl; The working concentration of acid is 1vol% ~ 5vol% (concentration expressed in percentage by volume); Alkali comprises: NaOH; Working concentration 1wt% ~ the 5wt% (concentration expressed in percentage by weight) of alkali; The temperature of soaking is room temperature, and the time of immersion according to the concentration of harmful substances contained in used carrier, can be generally 1h ~ 12h.
Described step 2) in, the method for load, comprising: dipping or sprinkling.
Described step 2) in, in the bacterium liquid of aerobic microbiological combination bacterium, also need to add thickening material to increase the viscosity of bacterium liquid, flow out from porous support with the bacterium liquid preventing aerobic microbiological from combining bacterium and cause the loss of bacterium liquid, and being beneficial to storage and transport; Wherein, this thickening material can be conventional food thickener, as comprised: one or more in gelatin, agar, sodium alginate, carboxymethyl cellulose etc.The consumption of thickening material is the 1wt% ~ 10wt% (concentration expressed in percentage by weight) of the bacterium liquid measure of aerobic microbiological combination bacterium, is preferably 1wt% ~ 5wt% (concentration expressed in percentage by weight).
Moreover, the invention also discloses a kind of application of biological products containing aerobic microbiological combination bacterium, as described in the application of biological products in sewage disposal.Wherein, described sewage comprises: municipal effluent and aquaculture wastewater (as aquiculture sewerage).
The step of described application in sewage disposal comprises: by the biological products containing aerobic microbiological combination bacterium with the form of fixed bed (as fixed with support) or fluidized-bed (as biological products being divided in floating use in hollow plastic ball), drop into and wait to need to carry out in the aerobic reaction pond of sewage disposal, thus carry out the aerobic treatment of sewage; Wherein, the input amount of biological products is generally 10vol% (the volume percent) ~ 50vol% of aerobic reaction pool volume, is preferably 20vol% ~ 30vol%.
The step of described application in sewage disposal, also can comprise: be also provided with ultraviolet lamp sterilising plant using the sewage outlet end of the biological products process aquaculture wastewater containing aerobic microbiological combination bacterium, namely after using the indices of above-mentioned biological products process aquaculture wastewater all to reach the standard of water used for aquiculture, also need ultraviolet lamp sterilising plant is installed at the sewage outlet end through aerobic treatment, to prevent in the water after processing containing growing disadvantageous microorganism or germ to fishery products.
The present invention is according to the feature of municipal effluent, by the method for enrichment, separation, domestication from the mud that nature exists, filter out the COD that efficiently can reduce sewage pointedly, the aerobic microbiological flora of ammonia nitrogen can be reduced again, and by these dominant microflora reasonable compatibilities together, appendix on porous support, is made and a kind ofly can, efficient for the biological products of sewage aerobic process, be prevented highly effective aerobic flora in use to run off.The concrete beneficial effect of these biological products is as follows:
1) namely biological products of the present invention can make i.e. use, as transported for long-distance or preserve, also can pack with after the plastics bag oxygenation of inflation, to extend the survival time of bacterium, keep the activity of flora.
2) biological products energy quick start of the present invention, greatly reduces domestication and initial failure period.
3) biological products of the present invention can replace active sludge, for the process of municipal effluent, and significantly can improve the efficiency of biochemical treatment, and efficient removal COD and ammonia nitrogen, improve effluent quality.
4), after adopting biological products of the present invention, the output of excess sludge can be made to reduce more than 80%.
Embodiment
In following examples, the biological products containing aerobic microbiological combination bacterium are prepared by the following method:
1) pseudomonas putida adopted is separated by method of scoring from insecticide factory's mud, then by its Fast-propagation in LB nutrient solution, makes bacterial concentration reach 10
8individual/more than ml.Through DNA analysis, confirm as pseudomonas putida (Pseudomonas putida strain CDd-9).
2) subtilis is separated to obtain from the mud of food processing plant.Sample kills genus bacillus nourishing body and other microorganism cells through heating, is then separated by scribble method.Flora cultivates about 20h in the nutrient solution prepared with glucose, peptone, sodium-chlor, extractum carnis and agar, and culture temperature is 3 DEG C.After phosphoric acid buffer centrifuge washing, record bacterial concentration and reach 10
8individual/more than mt, and through DNA analysis, confirm as subtilis.
3) above-mentioned two kinds of bacterium liquid are mixed in proportion, are mixed with mixed bacteria liquid, then, then be carried on porous support, obtain the biological products containing aerobic microbiological combination bacterium.
In following examples, agents useful for same if not otherwise specified, is commercialization reagent.
Now be described in detail by specific embodiment more.
Embodiment 1
The present embodiment porous support used is urethane foam, and its mean pore size about 0.5 ~ 1mm, average-volume is 20cm
3.First before using is soak 3h in the HCl solution of 1% (concentration expressed in percentage by weight), to remove residual isocyanic ester in concentration.Then, be washed till neutrality with clear water, extract for subsequent use.
Be l0 by the bacteria concentration prepared
8in the aerobic microbiological composite bacterial solution (subtilis and Pseudomonas putida bacterial content are 1:2) of individual/ml, add municipal effluent 10% (volume percent) every day, cultivate at normal temperatures, tame 5 days.Then take out 200mL bacterium liquid, add 3wt% Na-alginate, be uniformly dissolved, to increase the viscosity of mixed bacteria liquid, then, add volume be 2L through above-mentioned clean carrier, appendix is in the carrier (pickling process load) as far as possible equably to make bacterium liquid.The average appendix amount of total thalline of the bacterium liquid on every block carrier is about 10
7individual/cm
3.Thus the biological products obtained containing aerobic microbiological combination bacterium.
The test of the biological products process city domestic sewage containing aerobic microbiological combination bacterium of the present invention is used to carry out in homemade small-scale sewage handler (small testing device).Adopt A/O technique, i.e. anoxic+Aerobic Pond.Anoxic pond volume is 10L (100*200*500mm), and Aerobic Pond volume is 20L (200*200*500mm).With fixed bed (as fixed with support), the biological products about 20% to 50% (volume) containing aerobic microbiological combination bacterium are installed respectively in pond.Anoxic pond installs a pony mixer, and controlling rotating speed is 100 turns/min.Aerobic Pond adopts micro-pore aeration, controls dissolved oxygen concentration and is not less than 4mg/L.Lab scale sewage used takes from municipal wastewater treatment plant.The average COD of water inlet is about 250mg/L, and ammonia nitrogen is about 30mg/L.
During test, biological products are first allowed to tame in the city domestic sewage taking from Sewage Plant.Under the condition keeping effluent quality, progressively increase the water yield, after reaching predetermined volumetric loading, namely every day every M
3the clearance of volume COD is 0.6kg, hydraulic detention time HRT=8h.Constant amount of inlet water, and timing measures water-in and water-out water quality.
Test shows, applies the biological products Treating Municipal Sewage containing aerobic microbiological combination bacterium of the present invention, substantially can reach the index of integrated wastewater discharge standard (GB8978-1996) 1 grade.Its distinguishing feature is, stable, essentially no mud.
Embodiment 2
The present embodiment porous support used is urethane foam, and aperture is about 0.5mm, and every block amasss about 25cm
3.First before using is soak 2h in the HCl solution of 1% (concentration expressed in percentage by weight) in concentration, to remove residual S compound etc.Then, be washed till neutrality with clear water, dry for subsequent use.
Be 10 by the often kind of bacteria concentration prepared
85g Na-alginate is added in the aerobic microbiological composite bacterial solution 200ml (subtilis and Pseudomonas putida bacterial content are 1:2) of individual/ml, after being uniformly dissolved, add volume be 2L through above-mentioned clean carrier, appendix is in the carrier as far as possible equably to make bacterium liquid.The average appendix amount of total thalline of the bacterium liquid on every block carrier is about 10
7individual/cm
3, thus obtain the biological products containing aerobic microbiological combination bacterium.
Said products is arranged in the small testing device of embodiment 1, tests after running one week.The testing method of COD is with reference to GB GB11914-89, and Ammonia-nitrogen analysis method is with reference to GB GB7479-87.
Result is as follows:
Water yield 180L/ days, hydraulic detention time HRT=8h.
Water inlet: COD=150 ~ 500mg/L, average 250mg/L; Ammonia-nitrogen content=30 ~ 50mg/L; Average 40mg/L
Water outlet: COD=30 ~ 55mg/L, average 45mg/L; Ammonia-nitrogen content=0.14 ~ 1.5mg/L, average < 1.0mg/L
Second pond detects without mud.
Embodiment 3
This experimental example porous support used is cinder, and particle volume is 4 ~ 10cm about
3not etc.Aperture be 0.1 ~ 4mm not etc.The NaOH solution of front 1vol% is used to soak 12 hours, then with clear water rinsing to neutral and dry.Then, by the biological products of the method preparation described in embodiment 2 containing aerobic microbiological combination bacterium, and the technology and condition identical with embodiment 1 is adopted to carry out waste water dam.Result shows, the effect using these two kinds of vehicle treated sewage to obtain is similar.
Water yield 180L/ days, hydraulic detention time HRT=8h.
Water inlet: COD=150 ~ 500mg/L, average 250mg/L; Ammonia-nitrogen content=30 ~ 50mg/L; Average 40mg/L
Water outlet: COD=30 ~ 55mg/L, average 45mg/L; Ammonia-nitrogen content < 1.5mg/L.
Second pond detects without mud.
Embodiment 4
The biological products containing aerobic microbiological combination bacterium (namely containing the carrier of the aerobic microbiological combination bacterium) 1M that this experiment adopts embodiment 2 used
3, but subtilis and Pseudomonas putida bacterial content are 1: 1.Be arranged on 10M
3aerobic treatment pond in.The sewage of process is aquiculture sewerage.Influent COD is about 30mg/L, and ammonia-nitrogen content is about 1mg/L.Flooding quantity is 10M
3/ h, hydraulic detention time HRT=1h.After aerobic treatment, water outlet COD < 5mg/L, ammonia-nitrogen content is lower than 0.1mg/L.Water outlet uses through ultraviolet lamp disinfection Posterior circle.
Claims (10)
1. containing biological products for aerobic microbiological combination bacterium, it is characterized in that, comprising: the aerobic microbiological combination bacterium being carried on porous support; Wherein, aerobic microbiological combination bacterium comprises: genus bacillus and pseudomonas putida.
2. biological products as claimed in claim 1, is characterized in that: described porous support comprises: inorganic materials or macromolecular material; Wherein, inorganic materials comprises: one or more in gac, porous zeolite, coke, cinder; Macromolecular material comprises: porous plastics;
Porous support is of a size of 1cm
3~ 400cm
3, aperture is 0.1mm ~ 10mm.
3. biological products as claimed in claim 2, is characterized in that: described porous plastics comprises: one or more in urethane foam, polyvinyl chloride foam, polyvinyl alcohol foam plastics, ethylene vinyl acetate foam plastics, polymeric amide porous plastics, polyvinyl chloride foam; Porous support is of a size of 4cm
3~ 100cm
3, the aperture of porous support is 0.1mm ~ 5mm.
4. biological products as claimed in claim 1, is characterized in that: described porous support is urethane foam or cinder;
The proportioning of described genus bacillus and pseudomonas putida is 1: 1 ~ 1: 5;
Wherein, genus bacillus comprises: one or more in subtilis, bacstearothermophilus, bacillus pumilus, bacillus megaterium, bacillus cereus, Bacillus circulans;
The charge capacity of described aerobic microbiological combination bacterium is the bacterium liquid of load 0.1ml ~ 0.5ml aerobic microbiological combination bacterium on the porous support of every cubic centimetre; Wherein, aerobic microbiological combines the total cell concentration in the bacterium liquid of bacterium is 10
7individual/more than ml.
5. biological products as claimed in claim 4, is characterized in that: the proportioning of described genus bacillus and pseudomonas putida is 1: 1 ~ 1: 2;
Described genus bacillus is subtilis;
The charge capacity of described aerobic microbiological combination bacterium is the bacterium liquid of load 0.1ml ~ 0.2ml aerobic microbiological combination bacterium on the porous support of every cubic centimetre.
6. a preparation method for the biological products as described in any one of Claims 1 to 5, is characterized in that, comprises step:
1) after soaking porous support with acid or alkali, then with water rinse to neutral, pretreated porous support is obtained;
2) the bacterium liquid of aerobic microbiological as claimed in claim 1 combination bacterium is carried on through step 1) on the porous support that processes.
7. method as claimed in claim 6, is characterized in that: described step 1) in, acid comprises: mineral acid; Wherein, mineral acid comprises: the one in hydrochloric acid, sulfuric acid or its combination; The working concentration of acid is 1vol% ~ 5vol%;
Alkali comprises: NaOH; Working concentration 1wt% ~ the 5wt% of alkali;
The temperature of soaking is room temperature, and the time of immersion is 1h ~ 12h;
Step 2) in, in the bacterium liquid of aerobic microbiological combination bacterium, also add thickening material; Wherein, this thickening material comprises: one or more in gelatin, agar, sodium alginate, carboxymethyl cellulose; The consumption of thickening material is the 1wt% ~ 10wt% of the bacterium liquid measure of aerobic microbiological combination bacterium; The method of load, comprising: dipping or sprinkling.
8. method as claimed in claim 7, is characterized in that: the consumption of described thickening material is the 1wt% ~ 5wt% of the bacterium liquid measure of aerobic microbiological combination bacterium.
9. an application for the biological products as described in any one of Claims 1 to 5, is characterized in that: the application of described biological products in sewage disposal;
Wherein, described sewage comprises: municipal effluent and aquaculture wastewater;
The step of described application in sewage disposal comprises: by the biological products containing aerobic microbiological combination bacterium with the form of fixed bed or fluidized-bed, drop into and wait to need to carry out in the aerobic reaction pond of sewage disposal, thus carry out the aerobic treatment of sewage; Wherein, the input amount combining the biological products of bacterium containing aerobic microbiological is the 10vol% ~ 50vol% of aerobic reaction tank volume.
10. applying as claimed in claim 9, it is characterized in that: the step of described application in sewage disposal, also comprises: being also provided with ultraviolet lamp sterilising plant using the sewage outlet end of the biological products process aquaculture wastewater containing aerobic microbiological combination bacterium;
The input amount of the described biological products containing aerobic microbiological combination bacterium is the 20vol% ~ 30vol% of aerobic reaction tank volume.
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| CN106000314A (en) * | 2016-07-13 | 2016-10-12 | 浙江弘哲生物技术有限公司 | Microorganism-loaded activated carbon and preparation method thereof |
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| CN104974956A (en) * | 2015-06-25 | 2015-10-14 | 北京致清源环保科技有限公司 | Compound microorganism water-purification agent, preparation method and application |
| CN106000314A (en) * | 2016-07-13 | 2016-10-12 | 浙江弘哲生物技术有限公司 | Microorganism-loaded activated carbon and preparation method thereof |
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| CN106047854A (en) * | 2016-07-20 | 2016-10-26 | 申昱环保科技股份有限公司 | Preparation method of sewage treatment bactericide with rod-shaped activated carbon |
| CN106277351A (en) * | 2016-08-15 | 2017-01-04 | 卢生华 | A kind of sulphur bacteria is the method for main making microorganism hanging bag |
| CN107151058A (en) * | 2016-11-18 | 2017-09-12 | 上海果联国际贸易有限责任公司 | Microorganism adheres to porous body |
| CN107099483A (en) * | 2017-06-26 | 2017-08-29 | 金文忠 | A kind of composite biological agent and its application in processing mercury-containing waste water |
| CN107099483B (en) * | 2017-06-26 | 2020-09-18 | 杭州富阳佳畅机械有限公司 | Composite biological agent and application thereof in treatment of mercury-containing wastewater |
| CN111875423A (en) * | 2020-08-12 | 2020-11-03 | 莆田碧牧源农业开发有限公司 | Sludge aerobic fermentation organic fertilizer and preparation method thereof |
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Application publication date: 20150318 |