CN104403964A - Cultural method used for kitchen waste anaerobic digestion inoculation sludge - Google Patents

Cultural method used for kitchen waste anaerobic digestion inoculation sludge Download PDF

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Publication number
CN104403964A
CN104403964A CN201410608551.6A CN201410608551A CN104403964A CN 104403964 A CN104403964 A CN 104403964A CN 201410608551 A CN201410608551 A CN 201410608551A CN 104403964 A CN104403964 A CN 104403964A
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fermentation
culture
kitchen waste
anaerobic digestion
raw material
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徐锐
袁亚阁
段良飞
王秋侠
段欢耘
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Yunnan Normal University
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Yunnan Normal University
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/14—Fungi; Culture media therefor

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  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
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  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Virology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Botany (AREA)
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  • Processing Of Solid Wastes (AREA)

Abstract

The invention aims to provide a culture method used for kitchen waste anaerobic digestion inoculation sludge. The culture method is as follows: kitchen waste is collected to be used as a culture material, and bones, chopsticks, plastic bags and other debris are removed, and then the kitchen waste is crushed by a crusher; an original inoculum is taken from pond, sewer sediment or normal operation biogas digester fermentation residues; the appropriate fermentation concentration is selected, and the fermentation material and the inoculum are evenly mixed; the raw material is fed in batches, the daily feeding amount maintains the same, and the total raw material is fed in 10 to 15 days; before the start of culture, appropriate amount of additives are added into the fermentation liquor, the additives are activated carbon with the addition amount of 1g / L and aspergillus niger with the addition amount of 1g / L; before the start of culture, appropriate amount of nutritive salts (rich in K, P, Mg and other elements) are added into the fermentation liquor, the nutritive salts comprises KH2PO4 with the dosage of about 1360mg / L, CaCl2 with the dosage of 444mg / L and MgSO4 with the dosage of 500mg / L; after the start of the fermentation, stirring is kept on time every day, the pH value of the fermentation liquor is regulated by use of 0.2mol / L NaOH, and kept in about 7; and gas production end is regarded as the end of the culture, and the fermentation liquor in the tank is cultured mature anaerobic sludge.

Description

A kind of cultural method of the seed sludge for anaerobic digestion of kitchen wastes
Technical field
The present invention relates to a kind of cultural method being specifically designed to the high reactivity seed sludge of anaerobic digestion of kitchen wastes, belong to anaerobically digested sludge enrichment, cultivation and domestication field.
Background technology
Along with the increase of world population and the raising day by day of people's living standard, the output of changing food waste presents the trend rised appreciably in recent years.It is estimated, China only Domestic kitchen waste just has 1,610,000 tons of generations every year.Due to changing food waste moisture and organic content higher, so containing pathogenic microorganisms such as a large amount of Salmonellas, streptococcus aureuses, the insect such as fly, mosquito also can be grown in addition, if process not in time, also the hazardous and noxious substances such as dioxin may be produced, the life of harm people and environment.Existing changing food waste treatment technology has usually: pulverize in line, burning and burying, fodder, compost, anaerobic digestion techniques etc.Adopt anaerobic digestion techniques process changing food waste, be not only of value to environment protection, the recycling of changing food waste can also be improved.
Owing to having the feature such as environmental protection, energy, in recent years, the investigation and application of Anaerobic Digestion changing food waste more and more comes into one's own.From anaerobic digestion theory, the methanogenic process of organic anaerobic digestion has been come by multiple biogas microorganism, in anaerobic digestion process, the desired microorganisms adding q.s is very important as inoculum (also known as bacterial classification or anaerobic activated sludge).Effective constituent in inoculum is the biogas microbial population of living, so its activity of the inoculum of different sources is not identical, and effective constituent in inoculum and active height are directly connected to the quality that fermenting process runs.In engineering, if the inoculum activity added is too low, after will causing feeding intake start time long even at all can not normal fermentation.Particularly for the anaerobic digestion process taking changing food waste as raw material, if selected inoculum activity is not high, the very easily acidifying of its start-up course, thus the normal operation affecting anaerobic digestion.
But, the inoculum cultural method related in existing patent and document, the cultivation for mud required in a certain special process or reactor mostly, research for anaerobic digestion of kitchen wastes inoculum enrichment culture also rarely has report, so prior art can not meet the engineering demand of changing food waste high efficiency anaerobically fermenting completely.Therefore, the present invention aims to provide a kind of cultural method of the high reactivity seed sludge for anaerobic digestion of kitchen wastes.
Summary of the invention
Collect changing food waste as cultivation raw material, and reject the foreign material such as chopsticks, bone, plastics bag, pulverize through pulverizer.
Initial inoculum takes from pond, cloaca bed mud or normal operation methane-generating pit fermentation residues.
Choose concentration of suitably fermenting, fermentation raw material is mixed with inoculum.
The feeding mode of raw material adopts to be thrown in batches, and maintenance charging capacity every day is identical, total raw material is divided into input in 10 ~ 15 days.
Before cultivation starts, in fermented liquid, add appropriate additive, namely gac addition is 1g/L, and aspergillus niger addition is 1g/L;
Before cultivation starts, in fermented liquid, add adequate nutrition salt (being rich in the elements such as K, P, Mg), i.e. KH 2pO 4addition is 1360mg/L, CaCl 2addition is 444mg/L, MgSO 4addition is 500mg/L.
After fermentation starts, keep timing agitation every day, and adopt the NaOH of 0.2mol/L to regulate fermented liquid pH value, make it to remain on about 7.
Aerogenesis terminates namely to can be considered to cultivate and terminates, and in tank, fermented feed liquid is and cultivates ripe anaerobic sludge.
the present invention implements according to the following steps:
Collect changing food waste, reject the foreign material such as chopsticks, bone, plastics bag, pulverize through pulverizer.
Get pond, cloaca bed mud or the normal methane-generating pit fermentation residues that runs as inoculum.
Choose concentration of suitably fermenting, fermentation raw material is mixed with inoculum.
Fermentation raw material drops in fermentor tank in batches, and maintenance charging capacity every day is identical, continues to feed intake 10 ~ 15 days.
Before cultivation starts, in fermented liquid, add gac and aspergillus niger, addition is 1g/L; Before cultivation starts, in fermented liquid, add adequate nutrition salt, i.e. KH 2pO 4(1360mg/L), CaCl 2(444mg/L) and MgSO 4(500mg/L).
After fermentation starts, keep timing agitation every day, and adopt the NaOH of 0.2mol/L to regulate fermented liquid pH value, make pH remain on about 7.
Aerogenesis terminates namely to can be considered to cultivate and terminates, and in tank, fermented feed liquid is and cultivates ripe anaerobic sludge.
Accompanying drawing explanation
Fig. 1 is the culture apparatus of the anaerobic digestion of kitchen wastes mud that the present embodiment adopts, and this device is made up of 1-temperature controller, 2-alternating current contactor, 3-tank, 4-electrothermal tube, 5-thermopair, 6-water circulating pump, 7-marsh-gas fermentation tank, 8-opening for feed, 9-T-valve, 10-discharge port.
Fig. 2 is the fermentation gas effectiveness comparison before and after the present embodiment culturing sludge.
Fig. 3 is the statistical study of the fermentation results before and after the present embodiment culturing sludge.
embodiment:
Embodiment 1
Experiment in the present invention have employed the culture apparatus of anaerobically digested sludge.As shown in Fig. 1, this device by:
1-temperature controller, 2-alternating current contactor, 3-tank, 4-electrothermal tube, 5-thermopair, 6-water circulating pump, 7-
Marsh-gas fermentation tank, 8-opening for feed, 9-T-valve, 10-discharge port formed.
In the present embodiment, with certain sediment of pond for initial inoculum, the culture parameters adopted is as follows: have employed certain university's dining room changing food waste is raw material, the temperature of Water Tank with Temp.-controlled is 35 DEG C, fermentor tank effective volume 1.3L, raw material divides to be thrown in and keeps every day charging capacity identical for 15 days, and starting point concentration is feed liquid VS(volatile solid content) concentration 3 ~ 5%, cultivate and start to add gac (1g/L), aspergillus niger (1g/L), KH in forward direction feed liquid 2pO 4(1360mg/L), CaCl 2(444mg/L) and MgSO 4(500mg/L), every day at least timing agitation 1 time.
Aerogenesis terminates namely to be considered as cultivating to terminate, and can obtain and cultivate ripe anaerobic sludge.
Under identical fermentation condition, front to mud after cultivation and cultivation mud is carried out changing food waste biogas fermentation simultaneous test, compares the change to anaerobic digestion of kitchen wastes effect before and after culturing sludge.The aerogenesis situation of test as shown in Figure 2.
Fermentation results is as follows:
After cultivating, the maximum daily output tolerance of mud group fermentation is 908mL, cumulative gas production is 13361mL, raw material TS(total solids level) factor of created gase is 863.67mL/g, fermentation period is 57 days, and the 12nd day cumulative gas production accounts for total cumulative gas production about 50%, and (fermentation time is now expressed as T 50%), the 36th day cumulative gas production account for total cumulative gas production about 80% (fermentation time is now expressed as T 80%), illustrate that anaerobic digestion mainly occurs in first 36 days.Cultivate before mud group fermentation maximum daily output tolerance be 626mL, cumulative gas production is 12394mL, and raw material TS factor of created gase is 801.16mL/g, and fermentation period is 59 days, T 50%be 19 days, T 80%be 40 days, illustrate that anaerobic digestion mainly occurs in first 40 days.Statistical analysis, two groups of test-results othernesses as shown in Figure 3: the maximum daily output tolerance of sludge anaerobic fermentation after cultivating, cumulative gas production and raw material TS aerogenesis all apparently higher than group before cultivation, fermentation period be less than cultivate before group (difference is not remarkable), and T 50%and T 80%be starkly lower than group before cultivating, illustrate that the mud after aforesaid method is cultivated can promote the degraded of raw material and the carrying out of fermentation, improve raw material factor of created gase, relatively shorten fermentation time, namely mud is significantly improved for the fermentation activity of changing food waste.

Claims (1)

1. raw material is divided into input in 10 ~ 15 days, and gac addition is 1g/L, and aspergillus niger addition is 1g/L; Before cultivation starts, in fermented liquid, add adequate nutrition salt (being rich in the elements such as K, P, Mg), i.e. KH 2pO 4addition is 1360mg/L, CaCl 2addition is 444mg/L; MgSO 4addition is 500mg/L.
CN201410608551.6A 2014-11-04 2014-11-04 Cultural method used for kitchen waste anaerobic digestion inoculation sludge Pending CN104403964A (en)

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Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN108745294A (en) * 2018-06-01 2018-11-06 太原理工大学 A kind of method that fungi-kitchen garbage mixing solid phase fermentation prepares heavy metal absorbent
CN117660203A (en) * 2023-12-18 2024-03-08 河北省科学院生物研究所 Anaerobic fermentation promoter and preparation and application thereof

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103710158A (en) * 2014-01-02 2014-04-09 华南农业大学 Method for producing biodiesel by using kitchen wastes

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103710158A (en) * 2014-01-02 2014-04-09 华南农业大学 Method for producing biodiesel by using kitchen wastes

Non-Patent Citations (4)

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Title
冯磊等: "餐厨垃圾中温厌氧发酵接种污泥的驯化研究", 《可再生能源》 *
潘云霞等: "接种物浓度对厌氧发酵产气特性影响的研究", 《农机化研究》 *
胡晓明等: "黑曲霉处理稻草秸秆提高沼气发酵产气率的研究", 《云南师范大学学报》 *
陈佳一: "农作物秸秆厌氧发酵的接种物驯化特性研究", 《中国优秀硕士学位论文全文数据库工程科技I辑（电子期刊）》 *

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN108745294A (en) * 2018-06-01 2018-11-06 太原理工大学 A kind of method that fungi-kitchen garbage mixing solid phase fermentation prepares heavy metal absorbent
CN117660203A (en) * 2023-12-18 2024-03-08 河北省科学院生物研究所 Anaerobic fermentation promoter and preparation and application thereof

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Application publication date: 20150311